Product Catalog

Life Science Research

2022

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 Overview
Life Science Research 2021

Personalized Workflows for Drug Discovery and Development ii

COVID-19 Solutions xvi

New Products xviii

Sample Preparation 1

Sample Quantitation 19

Cell Counting 27

Flow Cytometry 31

Cell Imaging 75

Chromatography: Laboratory and Process Separations 79

Electrophoresis and Blotting 181

Antibodies 285

Imaging Instruments, Analysis, and Software 297

Bio-Plex Multiplex System 305

ELISA Reagents and Accessories 335

Transfection 341

DNA Amplification/PCR 355

Plastic Consumables and General Laboratory Equipment 425

Bio-Rad Explorer Educational Products 441

Appendices and Index 453

Note: Due to varying product timelines, some new items in this catalog may not be available for immediate purchase. Some older products have been discontinued since this catalog’s
creation. For more information, contact your local Bio-Rad sales representative.
 Personalized Workflows for
Translational Research
The journey of every therapeutic is unique. Bio-Rad offers a suite of customizable products
and services that ensure efficiency, reproducibility, and reliability at each step of discovery,
development, and manufacturing. With more than 60 years of experience as a global
leader in life science research and clinical diagnostics, Bio-Rad provides the highest-quality
integrated platforms and solutions so that you can focus on your science. Products are
manufactured in ISO 13485–certified production facilities and an experienced global support
team is available to partner with you to provide technical and regulatory audit support.

More than products: A trusted global partner

Bio-Rad’s commitment to your success does not end with your purchase. Our global
support and service team is dedicated to limiting downtime and maximizing your productivity.

Bio-Rad headquarters

Bio-Rad workflows — Bio-Rad understands the power of an optimized workflow: systems,

reagents, and software that work better together to enhance productivity and promote
confidence in your science. Over the next few pages, explore examples of the various
workflows Bio-Rad has to offer.

ii | Personalized Workflows for Translational Research

Customizable products and services from
discovery to approval and release

Target Lead Preclinical Clinical Process Manufacturing

Discovery Discovery Research Research Development

Biomarker
Discovery
and Validation

Protein-Based Biosimilar
Immunotherapy Development
Development and
Manufacturing

Cell and
Gene Therapy
Discovery and
Manufacturing

Personalized Workflows for Translational Research | iii

 Gene Editing Workflow

1
TRANSFECTION ENRICHMENT AND
2
SINGLE-CELL ISOLATION
Successful gene editing starts with delivering the Speed up your workflow, reduce passaging,
clustered regularly interspaced short palindromic and minimize unnecessary spending on
repeats with associated protein 9 (CRISPR-Cas9) reagents and consumables by enriching
system to your cells of interest, whether animal transfected populations for your cells of interest.
or human, tumor-derived or wild-type. From With the S3e cell sorter and ZOE fluorescent
the Gene Pulser Xcell electroporation system to cell imager, enrichment is easily performed at
TransFectin lipid reagent, Bio-Rad offers industry- the bench without the need for a core facility,
leading solutions for transfection of even the shortening your workflow by up to 30 days.
most sensitive cell lines. Automate cell counting
with the TC20 automated cell counter and use
the ZOE fluorescent cell imager to monitor cell
health right on your bench.

Gene Pulser Xcell TC20 automated cell counter, S3e cell sorter, ZOE fluorescent cell imager,
electroporation system, p. 28 p. 32 p. 76
p. 346

Related Products Related Products

Helios gene gun system, Gene Pulser electroporation TC20 automated cell counter, Flow cytometry antibodies,
p. 350 buffer, p. 345 p. 28 pp. 286, 294
Gene Pulser/MicroPulser ZOE fluorescent cell imager, Cell counting slides for TC20 ReadiLink antibody labeling
electroporation cuvettes, p. 76 cell counters, p. 29 kits, p. 48
p. 348 TransFectin lipid reagent, S3e biosafety system
p. 344 class I, p. 35

iv | Personalized Workflows for Translational Research bio-rad.com

 Gene Editing Workflow

3
CONFIRMATION OF EDITS DOWNSTREAM ANALYSIS
4
Confirmation of successful gene editing is Bio-Rad provides tools to study your newly
a key step in any CRISPR-Cas9 workflow. generated cell lines at the cellular, genomic, and
Bio-Rad offers a spectrum of solutions for direct proteomic levels. The flexible ZE5 cell analyzer
detection of edits by high-fidelity PCR, real-time can be run by novices and experts alike to study
PCR, and high resolution melt analysis. For the effects of your edits at the cellular level.
unmatched precision and sensitivity, leverage Save time by analyzing up to 100 biomolecules
Droplet Digital PCR (ddPCR) to identify mutations in a single sample with the Bio-Plex 200 system.
present even at frequencies ≤0.5%. For
confirmation at the protein level, the Stain-Free
western blotting workflow offers fast and
accurate quantitation.

Stain-Free western blotting QX ONE Droplet Digital Bio-Plex assays, ZE5 cell analyzer,
workflow, PCR system, p. 313 p. 41
p. 238 p. 356

Related Products Related Products

ddPCR genome edit Precision melt supermix, CFX Opus real-time PCR ReadiLink antibody labeling
detection assays, p. 363 p. 400 system, p. 376 kits, p. 48
CFX Opus real-time PCR PrecisionAb validated QX ONE Droplet Digital PCR PrecisionAb validated
system, p. 376 western blotting antibodies, system, p. 356 western blotting antibodies,
CFX Maestro software, p. 287 PX1 PCR plate sealer, p. 287
p. 383 Antibodies, p. 286 p. 407 StarBright antibodies, p. 289
PX1 PCR plate sealer, C1000 Touch thermal cycler, PrimePCR assays, p. 386 Antibodies, p. 286
p. 407 p. 371 Aurum total RNA kits, p. 10 Flow cytometry antibodies,
PrimePCR assays, p. 386 iTaq universal supermixes, PureZOL RNA isolation pp. 286, 294
SsoAdvanced universal p. 393 reagent, p. 11 HuCAL® antibodies, p. 286
probes supermix, p. 391 iProof HF master mix, p. 405 Reliance one-step multiplex Bio-Plex 200 system, p. 307
EveryBlot blocking buffer, GelDoc systems, p. 298 RT-qPCR supermix, p. 395
p. 252 Stain-Free western blotting
workflow, p. 238

bio-rad.com Personalized Workflows for Translational Research | v

 Vaccine Development Workflow

1
DISCOVERY PROCESS DEVELOPMENT
2
Evaluating the efficacy of a candidate vaccine Method development is required to create
requires analysis of diverse proteins and cell and optimize a purification process that
types over time. With Bio-Plex assays, you produces a vaccine candidate with sufficient
can easily monitor multiple proteins at once purity and yield. Bio-Rad offers a variety
to determine whether the immune system of chromatography resins scalable from
is responding appropriately. For cell-based discovery to production. These resins provide
analysis, Bio-Rad offers the ZE5 cell analyzer, purification benefits spanning a range of
which can measure up to 30 parameters at biomolecules used in vaccine development,
once and detect rare events and cell types. including new selectivity and high capacity to
The ZE5 cell analyzer is also automation-friendly improve recovery and purity. Additionally, the
for screening applications. NGC chromatography platform can be used
to automate purifications from discovery to
process development.

Bio-Plex assays, ZE5 cell analyzer, Resins, NGC chromatography

p. 313 p. 41 pp. 81, 176 systems,
p. 148

Related Products Related Products

Antibodies, p. 286 Flow cytometry antibodies, BioLogic LP systems, Process chromatography
Bio-Plex cytokine assays, pp. 286, 294 p. 170 resins, pp. 176, 177
p. 314 ReadiLink antibody labeling Stain-Free western blotting Foresight Pro columns,
kits, p. 48 workflow, p. 238 p. xix
ChemiDoc imaging systems, Stain-Free gels,
p. 299 pp. 198, 205
Foresight chromatography
resin screening tools,
p. 177

vi | Personalized Workflows for Translational Research bio-rad.com

 Vaccine Development Workflow

3
PURITY ANALYSIS QUALITY CONTROL
4
As you develop a purification scheme, you Host cell contamination is an area of primary
will need to assess the purity of the vaccine. concern in vaccine manufacturing. The QX200
The GS-900 calibrated densitometer is designed and QX ONE Droplet Digital PCR systems
for use in regulated environments and has provide semi-automated and automated
IQ/OQ and U.S. FDA 21 CFR Part 11 compliant methods for easy quantification of host cell
software tools. To ensure that the correct proteins DNA. Unlike quantitative PCR (qPCR), ddPCR
are expressed at the desired levels, Bio-Rad does not require a standard curve and provides
offers premade conventional and custom human an absolute readout, which is preferred for
recombinant antibodies for quantitative enzyme- reduction of interanalyst variability in quality
linked immunosorbent assays (ELISAs). control (QC). The Stain-Free western blotting
workflow can be used to confirm virus identity
and expression of surface proteins.

GS-900 calibrated HuCAL custom antibodies, QX ONE Droplet Digital Stain-Free western blotting
densitometer, p. 286 PCR system, workflow,
p. 302 p. 356 p. 238

Related Products Related Products

Stain-Free western blotting TMB peroxidase substrate ddPCR residual DNA TidyBlot reagent, p. 293
workflow, p. 238 kits, p. 338 quantification kits, p. 365 Clarity and Clarity Max ECL
Stain-Free gels, CFX Opus real-time PCR western blotting substrates,
pp.198, 205 system, p. 376 p. 255
CFX Maestro software, QX200 Droplet Digital PCR
p. 383 system, p. 357
PrecisionAb validated EveryBlot blocking buffer,
western blotting antibodies, p. 252
p. 287

bio-rad.com Personalized Workflows for Translational Research | vii

 Cell and Gene Therapy Workflow

1
FOUNDATIONAL BIOLOGY
2
PRECLINICAL/CLINICAL

Developing new cell or gene therapies starts Assessing the safety and efficacy of new
at the bench. The automation-friendly ZE5 therapies requires analyses at the cellular, nucleic
cell analyzer is capable of measuring up to acid, and protein levels. Leverage the power
30 parameters at once. For protein-based of the QX ONE Droplet Digital PCR system
assays, Bio-Rad offers premade antibodies and for absolute vector quantitation in prepared
antibody sets. When off-the-shelf antibodies therapies, treated cells, or treated patients. At
don’t meet your needs, try Bio-Rad’s custom, the cellular level, the ZE5 cell analyzer can be
fully human monoclonal antibodies from the used to monitor changes in specific immune cell
Human Combinatorial Antibody Libraries populations over time. Detect adverse effects
(HuCAL). HuCAL antibodies are highly specific with Bio-Plex assays, including predesigned
and can be generated in as little as 8 weeks, panels for simultaneous quantification of
saving valuable time. cytokines and other immune-related proteins.

ZE5 cell analyzer, HuCAL antibodies, QX ONE Droplet Digital Bio-Plex 200 system,
p. 41 p. 286 PCR system, p. 307
p. 356

Related Products Related Products

S3e cell sorter, p. 32 CFX Opus real-time PCR QX200 Droplet Digital PCR Reliance one-step multiplex
Flow cytometry antibodies, system, p. 376 system, p. 357 RT-qPCR supermix, p. 395
pp. 286, 294 PrimePCR assays, p. 386 CFX Opus real-time PCR Reliance Select cDNA
ReadiLink antibody labeling Aurum total RNA kits, p. 10 system, p. 376 synthesis kit, p. xxvi
kits, p. 48 PureZOL RNA isolation ZE5 cell analyzer, p. 41 Bio-Plex assays, p. 313
ReadyPrep protein extraction reagent, p. 11 PX1 plate sealer, p. 407 HuCAL antibodies, p. 286
kit, p. 3 Reliance Select cDNA PrimePCR assays, p. 386 Flow cytometry antibodies,
Stain-Free western blotting synthesis kit, p. xxvi SsoAdvanced universal pp. 286, 294
workflow, p. 238 Reliance one-step multiplex supermixes, pp. 390, 391
QX ONE Droplet Digital PCR RT-qPCR supermix, p. 395
system, p. 356

viii | Personalized Workflows for Translational Research bio-rad.com

 Cell and Gene Therapy Workflow

3A
MANUFACTURING/QC —
3B
MANUFACTURING/QC —
GENOMIC ASSAYS ANTIBODY-MEDIATED ASSAYS
As a new therapy moves toward regulatory High-quality antibody reagents are vital for
approval, manufacturing processes must be determining the percentage of transfected
developed and validated for reproducibility T cells prior to therapy administration and for
while maintaining product safety and efficacy. differentiating CAR T cells from other cells in
Measuring viral titers for gene therapy or vector patient samples using flow cytometry. Bio-Rad's
copy number for gene-modified cell therapy with custom antibody services use in vitro phage
high accuracy and precision is a challenge with display to generate highly specific antibodies
real-time PCR. Use the QX ONE Droplet Digital for these critical assays. These reagents can
PCR system, which provides absolute be reproduced synthetically for batch-to-batch
quantification, to deliver a reliable count of uniformity and long-term security of supply.
vector genome copies. In addition, Bio-Rad Additionally, Bio-Rad's incorporation of SpyTag
offers ddPCR residual DNA quantification kits technology to make TrailBlazer Antibodies
for checking purity with maximum sensitivity. enables production of multiple formats in less
than 3 months for use in multiple applications.

QX ONE Droplet Digital Vericheck ddPCR TrailBlazer Antibody HuCAL antibodies,

PCR system, Mycoplasma detection kit, Custom Service, p xx p. 286
p. 356 pp. xxvi, 367

Related Products Related Products

QX200 Droplet Digital PCR ddPCR Enhancer Reagent A, PrecisionAb validated Flow cytometry antibodies,
system, p. 357 p. xxiv western blotting antibodies, pp. 286, 294
ddPCR residual DNA Vericheck ddPCR HEK293 p. 287 StarBright antibodies, p. 289
quantification kits, p. 365 Res DNA Quant kit, p. xxvi Antibodies, p. 286
ddPCR supermix for residual C1000 Touch thermal cycler, Process-scale
DNA quantification, p. 361 p. 371 chromatography resins,
ddPCR cell and gene therapy pp. 176, 177
assays, p. xxiii

bio-rad.com Personalized Workflows for Translational Research | ix

 Protein Immunotherapy Workflow

1
IMMUNE CELL ANALYSIS IMMUNE PROTEIN MONITORING
2
To fully understand the effects of an Gaining insight into the roles proteins, like
immunotherapy, researchers must often monitor cytokines, play in the immune system drives the
multiple pathways and cell types, which requires development of the most effective treatments.
analytical tools that support multiparameter Monitoring these proteins with tools that offer
experiments. The fast and versatile ZE5 cell multiplexing, accuracy, and specificity allows you
analyzer can measure up to 30 parameters per to pursue complete answers. Bio-Plex assays
sample and can process a full 96-well plate in and systems can analyze many cytokines and
less than 15 minutes. You can easily assess the other critical proteins in a single experiment.
full range of effects of your therapeutic using Design a custom Bio-Plex assay panel for
Bio-Rad's extensive catalog of premade your unique needs, or save time with premade
conventional and custom human recombinant panels. For even more flexibility, browse Bio-Rad’s
antibodies. antibody library, including custom human
recombinant antibodies.

ZE5 cell analyzer, Antibodies, Bio-Plex 200 system, Antibodies,

p. 41 p. 286 p. 307 p. 286

Related Products Related Products

S3e cell sorter, p. 32 StarBright antibodies, p. 289 Bio-Plex assays, p. 313 ReadyPrep protein extraction
S3e biosafety system class I, Flow cytometry antibodies, Stain-Free western blotting kit, p. 3
p. 35 pp. 286, 294 workflow, p. 238 TidyBlot reagent, p. 293
HuCAL antibodies, p. 286 ReadiLink antibody labeling HuCAL antibodies, p. 286 Clarity and Clarity Max ECL
Cell health products, kits, p. 48 StarBright antibodies, p. 289 western blotting substrates,
pp. 52, 65 p. 255
PrecisionAb validated
western blotting antibodies, EveryBlot blocking buffer,
p. 287 p. 252

x | Personalized Workflows for Translational Research bio-rad.com

 Protein Immunotherapy Workflow

3
GENOMIC ANALYSIS AND MANUFACTURING AND
4
BIOMARKER DISCOVERY QUALITY CONTROL
Genomics plays an important role in development, For therapeutics built using novel scaffolds or
testing, and monitoring of immunotherapeutics. with modifications, identifying the best resin may
The CFX Opus real-time PCR system can be be complex. Bio-Rad’s chromatography resins
used with custom-built or predesigned PrimePCR are designed for a wide range of applications
assays to measure multiple genes from many and are available in multiple formats to take you
samples at once. If absolute quantification is from development to commercial production.
desired, Droplet Digital PCR provides a solution Testing purity as manufacturing is scaled up
without the need for a standard curve. requires trusted, compliance-friendly tools.
The GS-900 calibrated densitometer delivers
protein-based purification electrophoresis data
within a regulatory framework with IQ/OQ and
U.S. FDA 21 CFR Part 11 compliant software.

CFX Opus real-time QX ONE Droplet Digital Resins, GS-900 calibrated

PCR system, PCR system, pp. 81, 175 densitometer,
p. 376 p. 356 p. 302

Related Products Related Products

PrimePCR assays, p. 386 SingleShot cell lysis RT-qPCR Foresight chromatography QX200 Droplet Digital PCR
ddPCR multiplex mutation kits, p. 399 resin screening tools, p. 177 system, p. 357
screening kits, p. 366 Reliance Select cDNA Process chromatography ddPCR residual DNA
ddPCR copy number synthesis kit, p. xxvi resins, pp. 176, 177 quantification kits, p. 365
determination assays, Reliance one-step multiplex Foresight Pro columns Image Lab software, p. 303
p. 363 RT-qPCR supermix, p. 395 p. xix
PX1 plate sealer, p. 407 SsoAdvanced universal QX ONE Droplet Digital PCR
supermixes, pp. 390, 391 system, p. 356

bio-rad.com Personalized Workflows for Translational Research | xi

 Biosimilar Development Workflow

1
PURIFICATION IMPURITIES
2
Biosimilar developers are challenged with To ensure the safety and efficacy of a
producing a product at a cost lower than that biopharmaceutical, contaminants must be
of the original biologic while sourcing a reliable identified and removed. Bio-Rad offers market-
stream of workflow components. Bio-Rad's leading technologies for sensitive and robust
NGC chromatography systems and selection detection of host cell DNA and proteins. The
of chromatography resins enable fast and easy QX ONE Droplet Digital PCR system provides
screening of methods and components when maximum sensitivity and absolute quantification
designing a purification strategy for biosimilars. of host cell DNA without the need for a DNA
NGC chromatography systems allow addition of extraction step, all in an automated system. For
new capabilities as you move from discovery to the detection of protein contaminants, western
process development. Expedite your analyses blotting is faster and more flexible than ever
using Stain-Free technology to visualize before with the Stain-Free western blotting
biomolecules in your gels and confirm protein workflow solutions.
transfer during western blotting.

NGC chromatography Chromatography resins QX ONE Droplet Digital ddPCR residual DNA
systems, and columns, PCR system, quantification kits,
p. 148 pp. 81, 176, 177 p. 356 p. 365

Related Products Related Products

Foresight chromatography PrecisionAb validated ddPCR supermix for residual HuCAL antibodies, p. 286
resin screening tools, p. 177 western blotting antibodies, DNA quantification, p. 361 Stain-Free western blotting
Process chromatography p. 287 Vericheck ddPCR workflow, p. 238
resins, pp. 176, 177 StarBright antibodies, p. 289 Mycoplasma detection kit, ChemiDoc imaging systems,
Foresight Pro columns ChromLab software, p. 156 pp. xxvi, 367 p. 299
p. xix Image Lab software, p. 303 Vericheck ddPCR HEK293 Trans-Blot Turbo transfer
ChemiDoc imaging systems, Res DNA Quant kit, p. xxvi system, p. 241
Stain-Free gels,
p. 299 pp.198, 205 Antibodies, p. 286 Membranes, p. 248
Antibodies, p. 286 StarBright antibodies, p. 289
PrecisionAb validated
western blotting antibodies,
p. 287

xii | Personalized Workflows for Translational Research bio-rad.com

 Biosimilar Development Workflow

COMPARABILITY
3 CHARACTERIZATION
4
Biosimilars must show comparability to their The biosimilar must be compared to the
originator biologic across multiple factors, reference product to ensure that there are no
including structure, function, and efficacy. clinically meaningful differences in terms of
Designing a cell-based assay to measure safety, efficacy, and immunogenicity. Reagent
comparability requires an understanding of the quality in this testing is key, because results
underlying mechanism of action of the originator form part of the totality of evidence required by
and the biosimilar. The ZE5 cell analyzer enables regulatory agencies. Bio-Rad’s antibiotherapeutic
analysis of up to 30 parameters from a single antibodies are developed using a guided
sample so that you can monitor multiple cell selection strategy enabling the generation
types and lineages at once. Isolate cell types of fully human, highly specific, inhibitory and
needed for your assays using the S3e cell sorter, noninhibitory anti-idiotypic antibodies and
then easily image cells right at your bench with specialized drug-target complex binders.
the ZOE fluorescent cell imager.

ZE5 cell analyzer, ZOE fluorescent cell imager, HuCAL antibodies, Anti-idiotypic antibodies,
p. 41 p. 76 p. 286 p. 286

Related Products Related Products

Bio-Plex 200 system, p. 307 Flow cytometry antibodies, Stain-Free western blotting Bio-Plex 200 system, p. 307
Bio-Plex assays, p. 313 pp. 286, 294 workflow, p. 238 Bio-Plex assays, p. 313
CFX Opus real-time PCR S3e biosafety system
system, p. 376 class I, p. 35

PrimePCR assays, p. 386 Antibodies, p. 286

PX1 plate sealer, p. 407 S3e cell sorter, p. 32

ReadiLink antibody labeling

kits, p. 48

bio-rad.com Personalized Workflows for Translational Research | xiii

 Biomarker Discovery Workflow

1
CELLULAR PROTEOMIC
2
The ability to uncover novel and rare cell Interrogating the proteomic profile of samples
types through multiparameter analysis allows can identify a myriad of potential biomarkers.
researchers to characterize cell populations The Stain-Free western blotting workflow
in greater detail and identify subpopulations is a validated and streamlined process that
that can serve as markers for disease. With allows fast and accurate quantitation of protein
the ZE5 cell analyzer, up to 30 parameters expression. Antibody selection and specificity are
can be analyzed simultaneously to distinguish also crucial to the performance of your proteomic
subtly different cell populations. Reliably assays. Bio-Rad’s HuCAL custom antibody
isolate single cells, even for rare cell types, technology can generate highly specific custom
with the S3e cell sorter, and perform traditional human antibodies in only 8 weeks. For plate-
immunocytochemistry and histochemistry based analysis, the Bio-Plex 200 system can be
studies for biomarkers in live or fixed cells with used with predesigned or customized biomarker
the benchtop ZOE fluorescent cell imager. assay panels for multiplexed protein analysis.

ZE5 cell analyzer, S3e cell sorter, Stain-Free western blotting Bio-Plex 200 system,
p. 41 p. 32 workflow, p. 238 p. 307

Related Products Related Products

Antibodies, p. 286 ZOE fluorescent cell imager, Bio-Plex assays, p. 313 ReadyPrep protein extraction
HuCAL antibodies, p. 286 p. 76 Antibodies, p. 286 kits, p. 3

ReadiLink antibody labeling StarBright antibodies, p. 289 TidyBlot reagent, p. 293

kits, p. 48 PrecisionAb validated Clarity and Clarity Max ECL
western blotting antibodies, western blotting substrates,
p. 287 p. 255
HuCAL antibodies, p. 286

xiv | Personalized Workflows for Translational Research bio-rad.com

 Biomarker Discovery Workflow

3
GENOMIC
4
SOLUTIONS BEYOND DISCOVERY

Gene expression levels and the presence of Each biomarker’s proposed downstream
specific genetic sequences are used as biomarkers application defines the extent to which it must be
for many diseases. Bio-Rad provides powerful validated before it can move forward. Whatever
tools for analyzing gene expression, including your validation pathway or downstream need,
the QX ONE Droplet Digital PCR system and the you must have access to a reliable stream of
CFX Opus real-time PCR system. CFX real-time assay components and quality controls. Bio-Rad
systems provide top performance, usability, and partners with you on your biomarker journey,
ease of data acquisition and analysis. Reduce providing solutions from early discovery to the
hands-on time by pairing predesigned PrimePCR clinic and beyond and meeting your needs for
panels available for over 100 pathways. For security of supply for critical materials.
applications challenged with low expression levels
or small sample sizes, ddPCR provides a powerful
solution with accuracy and precision and makes
advanced multiplexing simple and robust.

QX ONE Droplet Digital CFX Opus real-time Bio-Plex 200 system, HuCAL antibodies,
PCR system, PCR system, p. 307 p. 286
p. 356 p. 376

Related Products Related Products

QX200 Droplet Digital PCR SsoAdvanced universal Bio-Plex assays, p. 313
system, p. 357 supermixes, pp. 390, 391 ddPCR multiplex mutation
Droplet Digital PCR assays, SEQuoia complete stranded screening kits, p. 366
p. 363 RNA library prep kit, p. xviii Quality control products,
PrimePCR panels, p. 386 SEQuoia dual-indexed primer, bio-rad.com/translationalQC
SingleShot cell lysis RT-qPCR p. xviii Diagnostic antibodies,
kits, p. 399 SEQuoia RiboDepletion kit, bio-rad.com/translationalDA
Reliance Select cDNA p. xviii
synthesis kit, p. xxvi
Reliance one-step multiplex
RT-qPCR supermix, p. 395

bio-rad.com Personalized Workflows for Translational Research | xv

 COVID-19 Solutions
Bio-Rad continues to support the worldwide effort to fight severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2), the virus associated with coronavirus disease 2019 (COVID-19),
by developing the needed tools for diagnosis, surveillance, and vaccine efficacy.

IgM
RNA
IgG
IgA

Active Infection Postinfection

Molecular Test Antigen Test Serology Test

Diagnosis and Surveillance

Bio-Rad testing solutions for SARS-CoV-2 span the full course of the viral infection stages,
with options from active infection testing to postinfection antibody testing. Some products
have limited regional availability.

In addition, Bio-Rad provides researchers the tools needed to detect SARS-CoV-2 in

wastewater, study population seroprevalence, and characterize how new SARS-CoV-2
variants spread over time. Simultaneous profiling of antibody response against SARS-CoV-2
nucleocapsid, receptor binding domain, spike 1, and spike 2 viral antigens can be useful in
seroprevalence studies.

Vaccine Efficacy
With SARS-CoV-2 infections still occurring, the virus continues to evolve into more infectious
and transmissible variants, such as the Delta variant. Therefore, researchers are consistently
assessing the efficacy of COVID-19 vaccines and therapeutics against emerging strains.

Measuring the ability of neutralizing antibodies to inhibit infection by the arising variants of
concern is crucial in assessing the efficacy of COVID-19 vaccines and comparing effectiveness
of naturally acquired or vaccine-induced antibody response in postmarket surveillance studies.
The multiplex neutralization antibody assays measure SARS-CoV-2 neutralizing antibodies
against two wild types and 11 variants of receptor binding domain and spike 1 protein subunits.

xvi | Covid Solutions

Bio-Rad solutions for COVID-19 diagnosis, surveillance, and vaccine efficacy. Bio-Rad has launched a series of products, such as the Reliance
SARS-CoV-2 RT-PCR Assay Kits, the PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit, and the Bio-Plex Pro Human SARS-CoV-2 Variant
Neutralization Antibody 11-Plex Panel, to assist researchers by providing the needed tools to investigate and combat the COVID-19 pandemic.

We'll solve it. Together.

Bio-Rad has joined countless healthcare professionals, diagnostic laboratories, and
researchers to help combat the COVID-19 global pandemic. We are proud to be your partner in
delivering the tools needed to identify individuals exposed to SARS-CoV-2 while also providing
resources for research and surveillance.
Vaccine
Bio-Rad Solution Diagnosis Surveillance Efficacy

Reliance SARS-CoV-2/FluA/FluB RT-PCR Assay Kits, p. xxv ✓

Reliance SARS-CoV-2 RT-PCR Assay Kits, p. xxv ✓
SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit, p. xxvi ✓
PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit, p. xxiv ✓
PrimePCR SARS-CoV-2 Variant Assays for RT-qPCR, p. xxiv ✓ ✓
Bio-Plex Pro Human IgA SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, p. xxii ✓ ✓
Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, p. xxii ✓ ✓
Bio-Plex Pro Human IgM SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel, p. xxii ✓ ✓
Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody
11-Plex Panel, p. xxi ✓ ✓
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel,
p. xxi ✓ ✓
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay
Developer Kit, p. xxi ✓ ✓
Visit bio-rad.com/COVID19 for more information.

Covid Solutions | xvii

 bio-rad.com

New Products
Sample Preparation
SEQuoia RNA Library Prep Solutions
The latest advancement in RNA-Seq library preparation chemistry, SEQuoia RNA library prep solutions profile the whole
transcriptome with an easy and streamlined workflow. The unique patented enzymatic technology captures both long and
short RNAs. This superior performance is ideal for use with low-input and low-quality samples.
SEQuoia Complete Stranded RNA Library Prep Kit SEQuoia RiboDepletion Kit
A high-performance RNA-Seq library prep kit that A stand-alone, post-library prep ribosomal RNA depletion
efficiently captures all RNA subtypes in a single library, kit that minimizes the number of library constructs mapping
even from limited and low-quality formalin-fixed paraffin- to ribosomal RNA and retains more of the transcriptome.
embedded (FFPE) samples. It is compatible with all It is compatible with most RNA library prep kits, and the
Illumina® sequencing platforms and is integrated with flexible protocol enables depletion of multiple libraries in a
bioinformatics software for secondary data analysis. The single depletion reaction, saving significant time and cost.
streamlined workflow is less than 4 hours. For More Information
For More Information Web: bio-rad.com/SEQuoiaRiboDepletion
Web: bio-rad.com/SEQuoiaComplete

SEQuoia Dual Indexed Primers

High-quality, unique dual indexed (UDI) primer pairs
designed for use with SEQuoia complete stranded RNA
library prep kits. They are balanced for optimal multiplexed
sequencing results and available in 12 UDI or 96 UDI
configurations.
For More Information
Web: bio-rad.com/SEQuoiaComplete

Flow Cytometry
ProFlow Cell Filters
ProFlow cell filters are used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant
material, nuclei, etc. These cup-fitting, disposable filters are available in 30, 50, and 70 µm mesh sizes to suit different cell
types. One package contains 100 filters.
For More Information
Web: bio-rad.com/ZE5
Web: bio-rad.com/S3e

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Chromatography: Laboratory and Process Separations

Econo Alpha Empty Glass Chromatography Columns
Econo Alpha columns are our new family of empty column hardware for low- to medium-pressure chromatography. These
columns are convenient, easy-to-pack, and compatible with NGC systems and other chromatography systems. The fully
assembled columns are available in a range of dimensions to fit different applications.
For More Information
Web: bio-rad.com/econo-alpha

Foresight Pro Columns

Foresight Pro columns are prepacked chromatography columns used for production-scale manufacturing of biologics.
The columns are good manufacturing practice (GMP) ready and validated to maintain column performance after shipping.
The columns are available with inner diameters from 5 to 33 cm and column bed heights of 10 or 20 cm for process
development to manufacturing. The column connectors use standard ½–3/4" Tri-Clamp connectors. These columns come
prepacked with Bio-Rad process chromatography resins.
For More Information
Web: bio-rad.com/foresightprocolumn

NGC Multi-Wavelength Detector II Module

The multi-wavelength UV/Vis detector adds flexibility to the NGC chromatography system, enabling the simultaneous
monitoring of four wavelengths (UV and Vis) from 190 to 800 nm for greater sensitivity and detection of proteins, peptides,
nucleic acids, and chromophores.
2 mm NGC Multi-Wavelength Detector II UV/Vis and 10 mm NGC Multi-Wavelength Detector II UV/Vis and
Conductivity Flow Cell Conductivity Flow Cell
Combined 2 mm UV/Vis and conductivity flow cell for the Combined 10 mm UV/Vis and conductivity flow cell for
NGC multi-wavelength detector II, compatible with F100 the NGC multi-wavelength detector II, compatible with F10
systems and ideal for use at high flow rates systems and ideal for use at lower flow rates
5 mm NGC Multi-Wavelength Detector II UV/Vis and
Conductivity Flow Cell
Combined 5 mm UV/Vis and conductivity flow cell for the
NGC multi-wavelength detector II, compatible with F10 and
F100 systems
For More Information
Web: bio-rad.com/MWDII

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Antibodies
Anti-BrdU Antibodies
These extensively validated antibodies for your proliferation studies show excellent performance in relevant biological
samples with appropriate controls. They have also been tested for cross-reactivity with thymidine and other thymidine
analogs.
For More Information
Web: bio-rad.com/brduabs

Rabbit Monoclonal Antibodies for Cancer Research

Bio-Rad offers a premium range of recombinant rabbit monoclonal antibodies against targets widely investigated in cancer
research. These include proteins involved in the AKT, EGF receptor, and NF- k B signaling pathways.
For More Information
Web: bio-rad.com/rabbitmono

Ready-Made Anti-Biotherapeutic Antibodies

Bio-Rad offers more than 100 antibodies against marketed biologic and biosimilar drugs. These antibodies are suitable for
the development of preclinical, clinical pharmacokinetic (PK), and anti-drug antibody (ADA) assays, and for therapeutic drug
monitoring. Our team of antibody experts has been making recombinant monoclonal antibodies targeting large molecule
biologics for more than 10 years, with 100% success for inhibitory anti-idiotypic antibodies. We are continually adding new
recombinant anti-idiotypic antibodies to help you increase productivity and deliver results.
For More Information
Web: bio-rad-antibodies.com/biotherapeutics

StarBright Dyes for Flow Cytometry

StarBright dyes are unique, proprietary, fluorescent nanoparticles conjugated to Bio-Rad’s highly validated antibodies.
They have been specially developed for flow cytometry to give exceptional brightness and are for use in multicolor panels in
immunophenotyping. The brightness allows easy resolution of rare populations and low-density antigens. In addition, these
dyes have the flexibility to fit into any multicolor panel without the need for special buffers. We will be introducing new dyes
throughout the year.
For More Information
Web: bio-rad-antibodies.com/starbright

TrailBlazer Antibody Custom Services

We have incorporated SpyTag technology into the Human Combinatorial Antibody Libraries (HuCAL®) antibody phage
display platform to offer TrailBlazer antibody custom services. This innovative combination enables site-directed conjugation
of antibodies with a controlled degree of labeling, which eliminates the risk of the label interfering with the binding site and
thereby improves assay reproducibility. All antibodies from a project can be supplied for testing in monovalent and bivalent
Fab formats, as well as in IgG-like formats, providing unprecedented flexibility in assay design.
For More Information
Web: bio-rad-antibodies.com/trailblazer

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Bio-Plex Multiplex System

Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays
Bio-Plex Pro Human SARS-CoV-2 neutralization antibody assays allow researchers to assess efficacy of COVID-19 vaccines
or compare the effectiveness of naturally acquired and vaccine-induced antibody response to arising variants of concern. The
highly precise assays quantitatively measure SARS-CoV-2 neutralizing antibodies against two wild-type and 11 variants (Alpha
S1, Beta S1, Delta RBD, Delta Spike Trimer, Gamma RBD, Epsilon RBD, Kappa RBD, D614G S1, E484K RBD, K417N RBD,
and N501Y RBD) of spike 1 (S1) and receptor binding domain (RBD) protein subunits. SARS-CoV-2 antigens are coated on
magnetic beads that bind neutralizing antibodies in competition with an ACE2 receptor that has been biotinylated for detection.
Bio-Plex Pro Human SARS-CoV-2 Variant Bio-Plex Pro SARS-CoV-2 Beta S1 Coupled Beads
Neutralization Antibody 11-Plex Panel Coupled magnetic beads for capturing neutralization
1 x 96-well, includes SARS-CoV-2 wild-type and variant antibodies that bind to SARS-CoV-2 Beta S1 viral antigen
coupled magnetic beads, biotinylated detection ACE2
Bio-Plex Pro SARS-CoV-2 Gamma RBD Coupled Beads
receptor, standard, ready-to-use positive control, assay
Coupled magnetic beads for capturing neutralization
buffer, wash buffer, serology sample diluent, streptavidin-
antibodies that bind to SARS-CoV-2 Gamma RBD viral antigen
phycoerythrin (SA-PE), 96-well flat bottom plate, and sealing
tape. For the detection of human neutralization antibodies Bio-Plex Pro SARS-CoV-2 Delta RBD and Spike Trimer
against SARS-CoV-2 RBD, S1, Alpha S1, Beta S1, 2-Plex Coupled Beads
Gamma RBD, D614G S1, E484K RBD, Epsilon RBD, Coupled magnetic beads for capturing neutralization
K417N RBD, Kappa RBD, and N501Y RBD antigens antibodies that bind to SARS-CoV-2 Delta RBD and spike
trimer viral antigens. Ready-to-use positive control is
Bio-Plex Pro Human SARS-CoV-2 Neutralization
included with the Delta 2-plex coupled beads
Antibody 2-Plex Panel
1 x 96-well, includes SARS-CoV-2 RBD and S1 coupled Bio-Plex Pro SARS-CoV-2 Epsilon RBD Coupled Beads
magnetic beads, biotinylated detection ACE2 receptor, Coupled magnetic beads for capturing neutralization
standard, ready-to-use positive control, assay buffer, antibodies that bind to SARS-CoV-2 Epsilon RBD viral antigen
wash buffer, serology sample diluent, SA-PE, 96-well flat Bio-Plex Pro SARS-CoV-2 Kappa RBD Coupled Beads
bottom plate, and sealing tape. For the detection of human Coupled magnetic beads for capturing neutralization
neutralization antibodies against SARS-CoV-2 wild-type antibodies that bind to SARS-CoV-2 Kappa RBD viral antigen
RBD and S1 antigens
Bio-Plex Pro SARS-CoV-2 D614G S1 Coupled Beads
Bio-Plex Pro Human SARS-CoV-2 Neutralization Coupled magnetic beads for capturing neutralization
Antibody Custom Assay Developer Kit antibodies that bind to SARS-CoV-2 D614G S1 viral antigen
Includes the Bio-Plex Pro human SARS-CoV-2 neutralization
antibody 2-plex panel, Bio-Plex amine coupling kit for Bio-Plex Pro SARS-CoV-2 E484K RBD Coupled Beads
coupling proteins to magnetic beads (includes bead wash Coupled magnetic beads for capturing neutralization
and activation buffers, storage and staining buffers, and antibodies that bind SARS-CoV-2 E484K RBD viral antigen
tubes), Bio-Plex Pro magnetic COOH beads (27), and Bio-Plex Pro SARS-CoV-2 K417N RBD Coupled Beads
Bio-Plex Pro serology beads storage buffer Coupled magnetic beads for capturing neutralization
Bio-Plex Pro Biotinylated Detection ACE2 Receptor antibodies that bind SARS-CoV-2 K417N RBD viral antigen
The biotinylated detection ACE2 receptor competes with Bio-Plex Pro SARS-CoV-2 N501Y RBD Coupled Beads
neutralizing antibodies in samples to bind the SARS-CoV-2 Coupled magnetic beads for capturing neutralization
RBD and S1 antigen coupled beads antibodies that bind to SARS-CoV-2 N501Y RBD viral antigen
Bio-Plex Pro SARS-CoV-2 Receptor Binding Domain Bio-Plex Pro SARS-CoV-2 Neutralization Antibody
Coupled Beads Standard
Coupled magnetic beads for capturing neutralization antibodies Neutralization antibody standard binds to SARS-CoV-2 viral
that bind to SARS-CoV-2 wild-type RBD viral antigen antigen, enough to generate a standard curve in duplicate
Bio-Plex Pro SARS-CoV-2 Spike 1 Coupled Beads for 1 x 96-well plate
Coupled magnetic beads for capturing neutralization Bio-Plex Pro SARS-CoV-2 Neutralization Antibody
antibodies that bind to SARS-CoV-2 wild-type S1 viral antigen Reagent Kit
Bio-Plex Pro SARS-CoV-2 Alpha S1 Coupled Beads 1 x 96-well, includes assay buffer, wash buffer, serology
Coupled magnetic beads for capturing neutralization sample diluent, SA-PE, 96-well flat bottom plate, and
antibodies that bind to SARS-CoV-2 Alpha S1 viral antigen sealing tape
For More Information
Web: bio-rad.com/bio-plexSARS-CoV-2NAb

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Bio-Plex Multiplex System (continued)

Bio-Plex Pro SARS-CoV-2 Serology Assays
Bio-Plex Pro SARS-CoV-2 serology assays provide COVID-19 researchers sensitive and specific assays to monitor the
antibody immune response profile against four viral protein subunits. There are three isotype-specific 4-plex panels
that measure antibodies against SARS-CoV-2 nucleocapsid, receptor binding domain, spike 1, and spike 2 viral protein
subunits. The isotype-specific antibodies detected are IgA, IgG, and IgM, giving the spectrum of antibody response after
COVID-19 vaccine administration or natural exposure to SARS-CoV-2.
Stand-alone components are available, allowing the flexibility to assay only the antibodies of interest. The viral protein
subunit–coated beads can be used by researchers studying animal models to assay antibodies in multiple species and
need only be paired with a species-specific biotinylated antibody for detection. Bio-Plex Pro reagent kits can be used to
further streamline assay configuration.
Bio-Plex Pro Human IgA SARS-CoV-2 Bio-Plex Pro Human IgM Detection Antibody
N/RBD/S1/S2 4-Plex Panel Biotinylated detection antibodies against IgM isotype
1 x 96-well, includes coupled magnetic beads, detection
antibody, positive control, negative control, assay buffer, Bio-Plex Pro SARS-CoV-2 Nucleocapsid
wash buffer, detection antibody diluent, SA-PE, flat bottom Coupled Beads
plate, sealing tape, and serology sample diluent. For the Coupled magnetic beads against SARS-CoV-2
detection of human IgA antibodies against SARS-CoV-2 N, N antibodies
RBD, S1, and S2 antigens Bio-Plex Pro SARS-CoV-2 Receptor Binding Domain
Bio-Plex Pro Human IgG SARS-CoV-2 Coupled Beads
N/RBD/S1/S2 4-Plex Panel Coupled magnetic beads against SARS-CoV-2
1 x 96-well, includes coupled magnetic beads, detection RBD antibodies
antibody, positive control, negative control, assay buffer, Bio-Plex Pro SARS-CoV-2 Spike 1 Coupled Beads
wash buffer, detection antibody diluent, SA-PE, flat bottom Coupled magnetic beads against SARS-CoV-2
plate, sealing tape, and serology sample diluent. For the S1 antibodies
detection of human IgG antibodies against SARS-CoV-2
N, RBD, S1, and S2 antigens Bio-Plex Pro SARS-CoV-2 Spike 2 Coupled Beads
Coupled magnetic beads against SARS-CoV-2
Bio-Plex Pro Human IgM SARS-CoV-2 S2 antibodies
N/RBD/S1/S2 4-Plex Panel
1 x 96-well, includes coupled magnetic beads, detection Bio-Plex Pro Human IgA SARS-CoV-2 Controls
antibody, positive control, negative control, assay buffer, IgA positive control and negative control
wash buffer, detection antibody diluent, SA-PE, flat bottom
Bio-Plex Pro Human IgG SARS-CoV-2 Controls
plate, sealing tape, and serology sample diluent. For the
IgG positive control and negative control
detection of human IgM antibodies against SARS-CoV-2 N,
RBD, S1, and S2 antigens Bio-Plex Pro Human IgM SARS-CoV-2 Controls
IgM positive control and negative control
Bio-Plex Pro SARS-CoV-2 N/RBD/S1/S2 4-Plex
Coupled Beads Bio-Plex Pro Human Serology Reagent Kit
Coupled magnetic beads against SARS-CoV-2 1 x 96-well, includes assay buffer, wash buffer, detection
N, RBD, S1, and S2 antibodies antibody diluent, SA-PE, flat bottom plate, sealing tape,
and serology sample diluent
Bio-Plex Pro Human IgA Detection Antibody
Biotinylated detection antibodies against IgA isotype
Bio-Plex Pro Human IgG Detection Antibody
Biotinylated detection antibodies against IgG isotype
For More Information
Web: bio-rad.com/bioplexsarscov2products

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DNA Amplification/PCR
CFX Duet Real-Time PCR System
The CFX Duet system is the newest entry point into our selection of CFX Real-Time PCR instruments. This new platform
offers the time-tested excellence of our CFX line with just the essentials: robust cycling and precision optics for experiments
with one or two colors.
For More Information
Web: bio-rad.com/cfxduet

CFX Opus 96 Dx and CFX Opus 384 Dx Real-Time PCR System

The CFX Opus 96 Dx and CFX Opus 384 Dx systems are open systems offering the ultimate flexibility in commercial assay
selection or rapid assay development in a clinical laboratory setting. The CFX Opus 96 Dx system can multiplex up to five
targets per well and the CFX Opus 384 Dx system can multiplex up to four targets per well. The instruments offer precise
quantification, accurate thermal performance, and CFX Maestro Dx, SE software, a powerful, easy-to-use software platform
that assists with U.S. FDA 21 CFR Part 11 compliance.
For More Information
Web: bio-rad.com/cfxopusdx

CFX Opus Deepwell Real-Time PCR System

The CFX Opus Deepwell system is the latest addition to our CFX Opus portfolio of real-time PCR systems. With all the
modern connectivity, multiplexing, and thermal performance of the existing CFX Opus systems, the CFX Opus Deepwell
system includes up to 125 µl reaction volume, making it the perfect choice for qPCR detection workflows requiring greater
reaction volumes.
For More Information
Web: bio-rad.com/cfxOpus

CFX Maestro Software 2.3

CFX Maestro software is an easy-to-learn and easy-to-use comprehensive suite of tools for collection and analysis of
real-time PCR data from the CFX series of real-time PCR systems. CFX Maestro software streamlines the process of plate
setup, data collection, data analysis, and data visualization of real-time PCR data.
CFX Maestro Software 2.3, Chinese Edition CFX Maestro Software 2.3, Security Edition,
Chinese version of CFX Maestro software, for real-time 1 License
PCR plate setup, data collection, statistics, and graphing Software for real-time PCR plate setup, data collection,
of results statistics, and graphing of results in a regulated environment
For More Information For More Information
Web: bio-rad.com/CFX-maestro-software-chinese-edition Web: bio-rad.com/CFX-maestro-software-security-edition

CFX Maestro Software 2.3, Russian Edition CFX Maestro Software 2.3, Security Edition,
Russian version of CFX Maestro software, for real-time 5 Licenses
PCR plate setup, data collection, statistics, and graphing Software for real-time PCR plate setup, data collection,
of results statistics, and graphing of results in a regulated environment
For More Information For More Information
Web: bio-rad.com/CFX-maestro-software-russian-edition Web: bio-rad.com/CFX-maestro-software-security-edition-5

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DNA Amplification/PCR (continued)

ddPCR Cell and Gene Therapy Assays
ddPCR cell and gene therapy assays include two sets of assays — vector backbone assays and gene of interest assays —
that enable accurate, precise, and robust viral titer measurements in a sample. They are orderable through the Cell and
Gene Therapy Design Engine (bio-rad.com/digital-assays) in multiple reaction sizes and fluorophore choices. The design
engine also offers in silico multiplex compatibility checks between a selected vector backbone assay and custom-designed
gene of interest assays for simultaneous titer measurements of two or more genes. Validation is recommended prior to use.
For More Information
Web: bio-rad.com/ddPCR-CellAndGeneTherapy

Droplet Digital PCR (ddPCR) Enhancer Reagent A

Enhancer Reagent A is used in conjunction with standard ddPCR materials in the ddPCR Whole Cell DNA Workflow. The
workflow enables accurate counting of cells containing genes of interest, edits, or knockouts by encapsulating whole cells
in droplets and subsequently performing Droplet Digital PCR amplification and analysis.
For More Information
Web: bio-rad.com/ddPCR-wholecell

PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit

The PREvalence ddPCR SARS-CoV-2 wastewater quantification kit provides reproducible, quantitative results in wastewater
testing for SARS-CoV-2. The PREvalence ddPCR SARS-CoV-2 wastewater quantification kit, based on the reverse
transcription PCR (RT-PCR) primers used by the Centers for Disease Control and Prevention (CDC) and World Health
Organization (WHO) for SARS-CoV-2 detection, allows for the detection of N2 and E genes in SARS-CoV-2. It also includes
an assay for the murine coronavirus, or mouse hepatitis virus (MHV), an enveloped, single-stranded RNA virus. MHV serves
as a matrix recovery control, enabling estimation of virus lost during sample processing. The kit allows for all three targets to
be detected in a single well. The kit contains reagents sufficient for 200 reactions.
For More Information
Web: bio-rad.com/PREvalence

PrimePCR Assays for SARS-CoV-2 Variant Surveillance Using Reverse Transcription Quantitative
PCR (RT-qPCR)
PrimePCR SARS-CoV-2 Multiple Mutation PrimePCR SARS-CoV-2 Variant Assays*
RT-qPCR Assays* Profile viral mutations associated with SARS-CoV-2
The SARS-CoV-2 multiple mutation assays offer the variants using new single and multiple mutation assays
ability to simultaneously detect five targets in one well. designed for RT-qPCR. To enable researchers to perform
Each assay includes primer/probe sets targeting four key rapid surveillance, these assays can be used in a one-step
mutations found in current variants of concern. Its unique RT-qPCR workflow in conjunction with the Reliance
multiplex control contains a primer/probe set designed to One-Step Supermix and positive and negative assay
specifically target a rarely mutated region of the SARS- controls from Bio-Rad. Validation is recommended prior
CoV-2 genome. to use.
PrimePCR SARS-CoV-2 Single Mutation Assays* SARS-CoV-2 variant-specific and SARS-CoV-2 standard
The SARS-CoV-2 single mutation assays contain primers (wild-type) positive controls, as well as a negative control,
and probe to simultaneously detect and differentiate a are available from Exact Diagnostics.
single mutation and one reference allele. Positive and * For research use only. Not for use in diagnostic procedures.
negative controls from Exact Diagnostics, a Bio-Rad
company, are available and recommended for assays that
target SARS-CoV-2 spike gene variant mutations.
For More Information
Web: bio-rad.com/SC2Variants

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DNA Amplification/PCR (continued)

The QX600 Droplet Digital PCR System
The QX600 Droplet Digital PCR system is a new six-color Droplet Digital PCR reader that delivers the same best-in-class
performance you’ve come to expect from Droplet Digital PCR systems, plus the addition of more channels for multiplexing
and the option of more partitions for better dynamic range and sensitivity. The QX600 system offers increased flexibility,
with the new capability of reading 100,000 droplets per well in a 96-well format. The QX600 system also supports all current
QX200 reagents and consumables designed for reading approximately 20,000 droplets per well in a 96-well format.
For More Information
Web: bio-rad.com/qx600

Reliance SARS-CoV-2 Testing Solutions

Reliance SARS-CoV-2 RT-PCR kits provide sensitive and robust detection of SARS-CoV-2 nucleic acid in diagnostic
testing laboratories. Choose from SARS-CoV-2–only formats, or respiratory panels targeting SARS-CoV-2, influenza A, and
influenza B. Each kit includes reagents, an internal amplification control, and assay controls in a convenient single package,
which can be stored at –20°C for 12 months (from date of shipment). The SARS-CoV-2 assay in both kits is designed to
target the nucleocapsid gene of SARS-CoV-2, and the performance of these assays is not affected by the Alpha, Beta,
Kappa, and Epsilon variants.
Reliance SARS-CoV-2 RT-PCR Assay Kit (EUA)* Reliance SARS-CoV-2/FluA/FluB RT-PCR Assay Kit
Reliable detection of SARS-CoV-2 nucleic acid is enabled (EUA)*
by targeting two regions, N1 and N2, of the nucleocapsid Sensitively detect and differentiate SARS-CoV-2, influenza
gene with the Reliance SARS-CoV-2 RT-PCR kit (EUA). A, and influenza B in a single sample with the Reliance
This kit uses the same primers/probe set as the Centers SARS-CoV-2/FluA/FluB RT-PCR Kit (EUA). The respiratory
for Disease Control and Prevention (CDC) kit, and can panel uses the same primers/probe set as the CDC
be run on both research use only (RUO) and in vitro Influenza SARS-CoV-2 (Flu SC2) multiplex assay. The
diagnostic (IVD) systems, including the CFX Opus 96, Reliance kit’s workflow enables labs to efficiently test
CFX Opus 384, CFX96 Dx, CFX96 Touch, and CFX384 multiple respiratory pathogens in a single sample, and
Touch Real-Time PCR Systems. can be run on RUO and IVD systems including the
For More Information
CFX Opus 96, CFX Opus 384, CFX96 Dx, CFX96 Touch,
Web: bio-rad.com/SARS-CoV-2-RTPCR-IVD and CFX384 Touch Real-Time PCR Systems.
For More Information
Reliance SARS-CoV-2 RT-PCR Assay Kit (IVD)**
Web: bio-rad.com/SARS-CoV-2FluAFluB-IVD
Reliable detection of SARS-CoV-2 nucleic acid is enabled
by targeting two regions, N1 and N2, of the nucleocapsid Reliance SARS-CoV-2/FluA/FluB RT-PCR Kit (IVD)**
gene with the Reliance SARS-CoV-2 RT-PCR assay kit (IVD). Sensitively detect and differentiate SARS-CoV-2, influenza
This product uses the same primers/probe set as the CDC A, and influenza B in a single sample with the Reliance
kit, and can be run on the CFX Opus 96 Dx, CFX96 Dx, and SARS-CoV-2/FluA/Flu B RT-PCR Kit (IVD). The respiratory
other IVD PCR systems. panel uses the same primers/probe set as the CDC
For More Information
Influenza SARS-CoV-2 (Flu SC2) multiplex assay kit. The
Web: bio-rad.com/SC2CE-IVD Reliance kit’s workflow enables labs to efficiently test
multiple respiratory pathogens in a single sample, and can
be run on the CFX Opus 96 Dx, CFX96 Dx, and other
IVD PCR systems.
For More Information
Web: bio-rad.com/SC2Flu-CE-IVD

* The U.S. FDA has granted emergency use authorization (EUA) for these kits in the U.S.
** These products are CE marked for sale outside the U.S., with limited regional availability. Please check with your local sales office.

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DNA Amplification/PCR (continued)

Reliance Select cDNA Synthesis Kit
This reverse transcription kit contains a novel chimeric enzyme that offers superior performance with degraded samples
such as formalin-fixed paraffin-embedded (FFPE) samples, inhibitors, and targets with high GC content. The 10 min reaction
protocol quickly and easily completes cDNA synthesis. The kit’s tolerance to common PCR inhibitors allows consistent RNA
quantification, even with inhibitor carryover, resulting in higher reproducibility. The kit is thermostable at 55ºC, so secondary
structures can be synthesized.
With its high versatility, you can stock your laboratory with this single reverse transcription kit.
For More Information
Web: bio-rad.com/rtreagents

SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit

The Bio-Rad SARS-CoV-2 ddPCR kit is a powerful diagnostic tool in the battle against COVID-19. This partition-based endpoint
real-time PCR test is intended for the qualitative detection of nucleic acids from SARS-CoV-2 in nasopharyngeal anterior nasal
and mid-turbinate nasal swab specimens, as well as nasopharyngeal wash/aspirate and nasal aspirate specimens from patients
suspected of having COVID-19 by their healthcare providers. The U.S. Food and Drug Administration (FDA) granted emergency
use authorization (EUA) for the Bio-Rad SARS-CoV-2 ddPCR kit in the U.S. on May 1, 2020.
For More Information
Web: bio-rad.com/SARS-CoV2ddPCRKit

Vericheck ddPCR HEK293 Res DNA Quant Kit

The Vericheck ddPCR HEK293 residual DNA quantification kit provides an extraction-free, reproducible, and host
cell–specific method to measure residual HEK293 DNA quantity. This sensitive method is designed and validated to
provide results that meet the current regulatory document requirements. The kit includes assay, supermix, positive
control, negative control, and an internal control.
For More Information
Web: bio-rad.com/ddPCR-Vericheck-HEK293Res

Vericheck ddPCR HEK293 Res DNA Size Kit

The Vericheck ddPCR HEK293 residual DNA sizing kit provides an extraction-free, reproducible, and host cell–specific
method to estimate the median size of residual HEK293 DNA fragments. It is designed and validated to meet the
requirements of regulatory documents. The kit includes assay, supermix, positive control, and negative control.
For More Information
Web: bio-rad.com/ddPCR-Vericheck-HEK293Res

Vericheck ddPCR Mycoplasma Detection Kit

The Vericheck ddPCR Mycoplasma detection kit is a probe-based, sensitive, and specific Mycoplasma detection
solution designed and validated to meet European, U.S., and Japanese pharmacopoeia requirements. It accurately
detects up to 112 Mycoplasma species using Droplet Digital PCR and utilizes the positive control–based, auto-
thresholding, U.S. FDA 21 CFR Part 11 compliant software for easy, turnkey data analysis.
For More Information
Web: bio-rad.com/ddPCR-vericheck-mycoplasma

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Bio-Rad Explorer Educational Products

DNA Barcoding Kits
Scientists use DNA barcoding to catalog the vast number of species on the planet. With these kits, students can identify
mammalian, insect, fungal, and fish species found in their own backyards. Your students will first extract DNA from tissue
they collect and then use PCR and electrophoresis to amplify and examine a genomic barcode region. After receiving DNA
sequence data from a third-party sequencing service (not included), they use bioinformatics to analyze the data and identify
the species. Students can also use samples from local shops to see if marketplace substitution is occurring!
For More Information
Web: bio-rad.com/DNAbarcoding

Out of the Blue CRISPR and Genotyping Extension Kits

Perform true CRISPR-Cas9 gene editing in your laboratory class! With the Out of the Blue CRISPR kit, students apply
CRISPR technology to disrupt the lacZ gene in E. coli and produce a visible phenotype upon blue-white screening. The Out
of the Blue CRISPR genotyping extension allows them to confirm the edit at the molecular level using PCR and agarose
gel electrophoresis. These kits use nonpathogenic, non–biofilm-forming bacteria and do not require specialized reagents
or equipment. The comprehensive curriculum includes background about the components of a CRISPR-Cas9 system, the
math behind its accuracy, and a guided bioinformatic analysis of CRISPR targets students design themselves.
For More Information
Web: bio-rad.com/outoftheblue

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 Sample Preparation
Protein Sample Preparation 2
Magnetic Beads and Racks DISCONTINUED
for Immunoprecipitation 2
Protein Extraction 3
Protein Sample Cleanup 4
Protein Fractionation 5
Protein Depletion 7

Nucleic Acid Sample Preparation 9

RNA Isolation 9
DNA Isolation 13
DNA Cleanup 15

Discontinued products are no longer available.

 Protein Sample Preparation
Magnetic Beads and Racks for Immunoprecipitation bio-rad.com/immunoprecipitation

Protein Sample Preparation

Magnetic Beads and Racks for Immunoprecipitation

See Also SureBeads Magnetic Beads System

Antibodies:
page 286.
Western blotting:
SureBeads are designed for bioseparation techniques
page 238. like immunoprecipitation (IP), co-immunoprecipitation
Chemiluminescence (co-IP), and protein pull-down assays. SureBeads
detection: page 255.
are superparamagnetic beads with surface activated
hydrophilic polymers and chemically conjugated
Protein A or Protein G that specifically bind to the Fc
region of immunoglobulins. Proprietary chemistry enables
high IgG binding and low nonspecific binding from a
variety of biological samples.
Product features include: For More Information
Web: bio-rad.com/immunoprecipitation
■
Faster IP — no centrifugation
Request or download bulletin: 6560
■
 asier IP — ergonomically designed magnetic rack
E
magnetizes beads in seconds, making washes easy Try TidyBlot western blot detection reagent to avoid
and preventing loss of beads during pipetting interference from IgG heavy or light chains when
■
 ess antibody needed — patented* surface
L detecting about 25 or 50 kD immunoprecipitated protein
chemistry enables proper antibody orientation for in western blots.
optimal antigen binding
■
M DISCONTINUED
 ore concentrated sample elution — small elution
volumes allow recovery of more concentrated sample
Tid Blot
■
Reproducible — consistent IgG binding capacity
ensures accurate, reproducible results
■
 ow cost to go magnetic — priced similarly to leading
L
agarose beads with the performance of magnetic beads
* U.S. patent 20100105879A1.

Ordering Information
Catalog # Description Price, U.S. $
1614011 SureBeads Protein A Magnetic Beads, 1 x 1 ml vial conjugated magnetic immunoprecipitation beads 83.00
(10 mg beads/ml suspension)
1614013 SureBeads Protein A Magnetic Beads, 1 x 3 ml vial conjugated magnetic immunoprecipitation beads 160.00
(10 mg beads/ml suspension)
1614021 SureBeads Protein G Magnetic Beads, 1 x 1 ml vial conjugated magnetic immunoprecipitation beads 83.00
(10 mg beads/ml suspension)
1614023 SureBeads Protein G Magnetic Beads, 1 x 3 ml vial conjugated magnetic immunoprecipitation beads 160.00
(10 mg beads/ml suspension)
1614916 16-Tube SureBeads Magnetic Rack, with separable magnets 345.00

2 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

Discontinued products are no longer available.
 Protein Sample Preparation
bio-rad.com/proteinsampleprep Protein Extraction

Protein Extraction
Protein extraction tools such as cell lysis and extraction kits, as well as mini grinders, are available for extracting proteins
from cultured cells and tissues.

ReadyPrep Protein Extraction Kit

The ReadyPrep protein extraction kit (total protein)

provides a simple, rapid, and reproducible method for
preparation of total cellular protein extracts from a wide
variety of biological samples. Use of this kit generates
protein samples that can be applied directly to a variety
of methods, including IEF and 2-D gel electrophoresis.
For More Information
Web: bio-rad.com/proteinextraction

Ordering Information
Catalog # Description Price, U.S. $
1632086 ReadyPrep Protein Extraction Kit (Total Protein), 20 preps 190.00

Kits for Cell Lysis See Also

Protein assay kits:
page 20.
Bio-Plex Cell Lysis Kit
This cell lysis kit is used to prepare cell lysates for western
blot analysis. Its protein extraction procedure yields western
blotting results similar to those generated by routine cell
lysis and protein extraction protocols.
For More Information
Web: bio-rad.com/proteinextraction and /bioplexcelllysis
Download bulletins: 3033 and 3034

ReadyPrep Mini Grinders

For grinding small biological samples for high recovery of
proteins (and nucleic acids), each mini grinder includes a
1.5 ml grinding tube containing a grinding resin and a
matching pestle. The grinding resin is a neutral abrasive
material made of high tensile strength microparticles that
do not bind to proteins or nucleic acids. ReadyPrep mini
grinders are disposable and are nuclease- and protease-
free. The mini grinder tubes fit conveniently in most
benchtop centrifuges.
For More Information
Web: bio-rad.com/proteinextraction

Ordering Information
Catalog # Description Price, U.S. $
1632146 ReadyPrep Mini Grinders, includes 20 mini grinders, sufficient for twenty 100 mg extractions 125.00
Bio-Plex Cell Lysis Kits
171304011 Bio-Plex Cell Lysis Kit, 1 x 96-well, includes cell lysis and wash buffers, factor 1 and factor 2 180.00
171304012 Bio-Plex Cell Lysis Kit, 10 x 96-well, includes cell lysis and wash buffers, factor 1 and factor 2 1,510.00

In the U.S., call toll free at 1-800-424-6723 Sample Preparation | 3

 Protein Sample Preparation
Protein Sample Cleanup bio-rad.com/proteinsampleprep

Protein Sample Cleanup

See Also General-purpose cleanup kits and columns are available for the removal of salts and other contaminants.
Bio-Spin gel
filtration columns: Protein Sample Cleanup Kit Selection Guide
page 16. Kit Type and Preparation Number of
Micro Bio-Spin Catalog Number Applications Procedure Time Preps
gel filtration
columns: page 16. Salt Removal
ReadyPrep 2-D cleanup Cleanup of protein samples for TCA-like precipitation to remove salts, <1 hr 50
kits (#1632130, #1632140) 1-D and 2-D applications detergents, lipids, phenolic compounds
Reduction of streaking on 2-D gels
Concentration of dilute samples
Micro Bio-Spin P-6 Removal of salts and other Size exclusion chromatography 5 min 25, 100
columns (see pages 16–17) contaminants
Removal of Other Contaminants
ReadyPrep 2-D cleanup Reduction of streaking on 2-D gels TCA-like precipitation to remove <1 hr 50
kits (#1632130, #1632140) Concentration of dilute samples salts, detergents, lipids, phenolic
compounds
Reduction and Alkylation
ReadyPrep reduction- Reduction of streaking on 2-D gels Reduction, then alkylation of sample <2 hr 100
alkylation kit (#1632090) Improved resolution of basic proteins to remove disulfide bonds and prevent
their re-formation

For More Information

Web: bio-rad.com/proteincleanup
Download bulletins: 2934 and 2961

Ordering Information
Catalog # Description Price, U.S. $
ReadyPrep Kits
1632130 ReadyPrep 2-D Cleanup Kit, 50 preps 315.00
1632140 ReadyPrep 2-D Cleanup Kit, 5 preps 125.00
1632105 ReadyPrep 2-D Starter Kit, includes protein sample and reagents sufficient to rehydrate, focus, and transfer 260.00
to second-dimension gels; six 17 cm, ten 11 cm, or sixteen 7 cm ReadyStrip IPG strips (ReadyStrip IPG
strips,* precast SDS-PAGE gels, and gel stains not included in kit)
1632090 ReadyPrep Reduction-Alkylation Kit, 100 preps 215.00
ReadyPrep Kit Components and Related Products
1632091 ReadyPrep Proteomics Grade Water, 500 ml 40.00
1632083 ReadyPrep 2-D Rehydration/Sample Buffer 1, 10 ml, 7 M urea, 2 M thiourea, 1% ASB-14, 40 mM Tris, 38.00
0.001% bromophenol blue
1632106 ReadyPrep 2-D Starter Kit Rehydration/Sample Buffer, 10 ml, 8 M urea, 2% CHAPS, 50 mM DTT, 44.00
0.2% Bio-Lyte 3/10 ampholyte, 0.001% bromophenol blue
Reducing and Alkylating Agents
1610611 Dithiothreitol (DTT), 5 g 81.00
1632101 Tributylphosphine (TBP), 200 mM, 0.6 ml 44.00
1632109 Iodoacetamide, 30 g 145.00
* See the prepacked Bio-Spin and Micro Bio-Spin gel filtration columns on pages 16–17.

4 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

 Protein Sample Preparation
bio-rad.com/proteinsampleprep Protein Fractionation

Protein Fractionation
Fractionation kits reduce sample complexity, helping to identify low-abundance proteins. Fractionation kits can be
subdivided into three groups that fractionate based on differential solubility, cellular location of the proteins of interest,
and protein charge.

Fractionation by Solubility

Fractionation Using Differential Protein Solubility visualization of proteins that might not otherwise be seen.
The ReadyPrep sequential extraction kit and the ReadyPrep Increasing solubilization strength is provided through the
protein extraction kit (soluble/insoluble) both reduce sample use of stronger detergents for each subsequent fraction
complexity using differential solubilization. The two kits ■
 eadyPrep protein extraction kit (soluble/insoluble) —
R
can be used independently, or the rehydration/sample uses a single fractionation step
buffer from the soluble/insoluble kit can be used with the
sequential extraction kit to create a fourth fraction for even ReadyPrep Kit Components and Related Products
better resolution. Individual ReadyPrep kit components and related products
such as reducing agents are also available.
■
 eadyPrep sequential extraction kit — enables the
R
isolation of 3 different fractions of increasing solubility. For More Information
Web: bio-rad.com/proteinfractionation
These fractions are isolated sequentially, allowing the
Download bulletins: 2934 and 2961

Ordering Information
Catalog # Description Price, U.S. $
ReadyPrep Kits
1632100 ReadyPrep Sequential Extraction Kit, 15 preps 215.00
1632085 ReadyPrep Protein Extraction Kit (Soluble/Insoluble), 20 preps 235.00
ReadyPrep Kit Components and Related Products
1632103 ReadyPrep Sequential Extraction Kit Reagent 2, 10 ml, 8 M urea, 4% CHAPS, 40 mM Tris 36.00
base, 0.2% Bio-Lyte 3/10 ampholyte
1632104 ReadyPrep Sequential Extraction Kit Reagent 3, 10 ml, 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3–10, 36.00
40 mM Tris base, 0.2% Bio-Lyte 3/10 ampholyte
1632083 ReadyPrep 2-D Rehydration/Sample Buffer 1 38.00

In the U.S., call toll free at 1-800-424-6723 Sample Preparation | 5

 Protein Sample Preparation
Protein Fractionation bio-rad.com/proteinsampleprep

Fractionation by Cellular Location

Fractionation by Cellular Location ■

 eadyPrep protein extraction kit (membrane II) —
R
ReadyPrep protein extraction kits facilitate fractionation offers a protocol for isolating more complex
of proteins from different cellular locations such as the membrane proteins
membrane, nucleus, or cytoplasm. ■
 eadyPrep protein extraction kit (signal) —
R
■
 eadyPrep protein extraction kit (cytoplasmic/
R for isolating proteins involved in intracellular membrane
nuclear) — prepares fractions enriched in cytoplasmic or trafficking and signaling pathways. These include GPI-
nuclear proteins from eukaryotic samples anchored proteins, caveolin and associated proteins,
acylated tyrosine kinases, and G proteins
■
 eadyPrep protein extraction kit (membrane I) —
R
offers a quick and effective protocol for isolating most For More Information
Web: bio-rad.com/proteinfractionation
membrane proteins. It does not require ultracentrifugation
Download bulletins: 2934 and 2961
or preparation of density gradients

Ordering Information
Catalog # Description Price, U.S. $
ReadyPrep Kits
1632089 ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps 450.00
1632088 ReadyPrep Protein Extraction Kit (Membrane I), 50 preps 450.00
1632084 ReadyPrep Protein Extraction Kit (Membrane II), 10 preps 270.00
1632087 ReadyPrep Protein Extraction Kit (Signal), 50 preps 230.00
Related Products
1632083 ReadyPrep 2-D Rehydration/Sample Buffer 1 38.00
1632085 ReadyPrep Protein Extraction Kit (Soluble/Insoluble), 20 preps 235.00

6 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

 Protein Sample Preparation
bio-rad.com/proteinsampleprep Protein Depletion

Protein Depletion
Complex samples often require depletion of high-abundance proteins to allow the detection of the low-abundance ones.
Bio-Rad offers two different methodologies for protein depletion — the ProteoMiner protein enrichment system
(which utilizes hexapeptide libraries) and Aurum serum and Affi-Gel Blue products (which utilize resins).

ProteoMiner Protein Enrichment System

The ProteoMiner protein enrichment system is a novel ProteoMiner Protein Enrichment Kits — these kits
sample preparation tool for reducing the dynamic range can be used with a variety of biological samples and
of protein concentrations in complex biological samples. are compatible with all major downstream proteomics
The ProteoMiner system: applications. Small- and large-capacity kits are available
■
Enriches and concentrates low-abundance proteins that for processing two or ten samples.
cannot be detected through traditional methods ProteoMiner Sequential Elution Kits — these kits utilize
■
 orks with a variety of sample types (serum, plasma, urine,
W multiple elution reagents to sequentially elute proteins
bile, cell lysates, tissues, and platelets) and is not limited by based on different properties.
the species’ origin
■
 tilizes a combinatorial library of hexapeptides rather
U
than immunodepletion, minimizing dependence on
available antibodies and preventing the codepletion of
low-abundance proteins

Apply sample Wash Elute

Proteins

ProteoMiner
beads

Flowthrough Wash Eluate

ProteoMiner technology is based on the interaction of complex protein samples with a large, highly diverse library of hexapeptides bound
to chromatographic supports. In theory, each unique hexapeptide binds to a unique protein sequence. Because the bead capacity limits binding
capacity, high-abundance proteins quickly saturate their ligands (red and yellow beads) and excess protein is washed out during the procedure. In
contrast, low-abundance proteins are concentrated on their specific ligands (pink and teal beads), thereby decreasing the dynamic range of proteins in
the sample. When analyzed in downstream applications, the number of low-abundance proteins detected is dramatically increased.

In the U.S., call toll free at 1-800-424-6723 Sample Preparation | 7

 Protein Sample Preparation
Protein Depletion bio-rad.com/proteinsampleprep

ProteoMiner Small- and Large-Capacity Kits ■

Small-capacity kits — optimized for use with
ProteoMiner kits for protein enrichment are now offered in limited sample material (minimum 10 mg of protein
formats optimized for varying starting amounts of is recommended)
sample protein. ■
Large-capacity kits — optimized for use with
samples in which at least 50 mg of protein is available
For More Information
Web: bio-rad.com/proteominer
Download bulletins: 5632, 5635, and 5841

Ordering Information
Catalog # Description Price, U.S. $
1633006 ProteoMiner Protein Enrichment Small-Capacity Kit, 10 preps, for processing 10 mg of total protein, 665.00
includes 10 spin columns, wash buffer, elution reagents, collection tubes
1633007 ProteoMiner Protein Enrichment Large-Capacity Kit, 10 preps, for processing 50 mg of total protein, 910.00
includes 10 spin columns, wash buffer, elution reagents, collection tubes
1633008 ProteoMiner Protein Enrichment Introductory Small-Capacity Kit, 2 preps, for processing 10 mg of total 215.00
protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes
1633009 ProteoMiner Protein Enrichment Introductory Large-Capacity Kit, 2 preps, for processing 50 mg of total 245.00
protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes
ProteoMiner Sequential Elution Kits
1633010 ProteoMiner Sequential Elution Small-Capacity Kit, 10 preps, for processing 10 mg of total protein, 805.00
includes 10 spin columns, wash buffer, 4 sequential elution reagents, collection tubes
1633011 ProteoMiner Sequential Elution Large-Capacity Kit, 10 preps, for processing 50 mg of total protein, 1,050.00
includes 10 spin columns, wash buffer, 4 sequential elution reagents, collection tubes
ProteoMiner Kit Accessories
1633003 ProteoMiner Sequential Elution Reagents, 10 preps, includes reagents only (columns not included), 300.00
to be used with #1633006 or #1633007

Aurum Kits and Columns

The Aurum Affi-Gel Blue and Aurum serum kits and Before After
columns use affinity chromatography to reduce albumin and
IgG, which improves analysis of lower abundance proteins.
Heavy- Heavy-
These products utilize a quick and easy spin-column format chain chain
Albumin Albumin
and provide eluted proteins ready for analysis. IgG IgG
Light- Light-
For More Information chain chain
Web: bio-rad.com/proteindepletion IgG IgG
Download bulletin: 2823
Removal of albumin and IgG from serum using the Aurum serum
protein mini kit. Total protein (1.32 mg) was purified on an Aurum
serum protein mini column. Left, untreated serum; right, serum treated
with the Aurum kit.

Ordering Information
Catalog # Description Price, U.S. $
7326701 Aurum Serum Protein Mini Kit, 10 pack, includes columns and buffers 525.00
7326708 Aurum Affi-Gel Blue Mini Columns, 25 pack 390.00

8 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

 Nucleic Acid Sample Preparation
bio-rad.com/nasampleprep RNA Isolation

Nucleic Acid Sample Preparation

RNA Isolation
Bio-Rad offers a broad range of sample preparation products for RNA isolation. Scalable and high-throughput products See Also
are available. PCR reagents:
page 388.
RNA Isolation Product Selection Guide Real-time PCR
systems: page 375.
Aurum Total RNA Kits PureZOL RNA
Experion automated
Mini Kit Fatty and Fibrous Tissue Kit 96 Kit Isolation Reagent
electrophoresis
Format Mini column Mini column 96-well plate Single solution organic system: page 277.
filtration (vacuum or spin) filtration (vacuum or spin) filtration (vacuum or spin) extraction DNA amplification/PCR:
page 355.
Maximum starting
material amounts
Cultured cells 2 x 106 1 x 107 1 x 106 1 x 107
Bacterial cells 2.4 x 109 2.4 x 109 8 x 108 2.4 x 109
Yeast cells 3 x 107 3 x 107 2 x 107 3 x 107
Hard animal tissue 20 mg 100 mg — 100 mg
Soft to moderately 40 mg 100 mg — 100 mg
hard animal tissue
Plant tissue 40 mg 100 mg — 100 mg
Isolation method Silica membrane Lysis with PureZOL reagent, Silica membrane Organic extraction
purification on silica membrane
Number of preps 50 50 192 (2 x 96-well plate) 50 or 100 (1 ml/prep)
Number of washes 3 3 3 —
DNase I included* Yes Yes Yes No
DNase I digest time 15 min (animal tissue, 25 min) 15 min 10 min —
Total preparation time** <50–80 min (with DNase I digest) <50–80 min (with DNase I digest) <60 min (with DNase I digest) <60 min
Binding capacity >100 µg >100 µg >40 µg —
Maximum elution
volume 80 µl (2 x 40 µl elutions) 2 x 40 µl 80 µl 30–100 µl
Yield*** 20–30 µg/2 x 106 NIH 3T3 cells 140–150 µg/1 x 107 293H cells 10 µg/1 x 106 NIH 3T3 cells 1.37 µg/1 x 105 293H cells
20–40 µg/2 x 106 HeLa cells 90–100 µg/100 mg brain 17 µg/1 x 106 HeLa cells 1.15 µg/mg brain
15–20 µg/40 mg brain 50–60 µg/100 mg breast 11 µg/2 x 107 S. cerevisiae cells 1.37 µg/mg fat
30–55 µg/20 mg thymus 80–90 µg/100 mg heart 5 µg/8 x 108 E. coli cells 1.2 µg/mg heart
15–30 µg/40 mg spleen 60–70 µg/100 mg skin 5 µg/8 x 108 B. cereus cells 0.66 µg/mg cartilage
8–15 µg/40 mg liver 50–60 µg/100 mg cartilage 1.2 µg/mg skin
15–20 µg/40 mg kidney 20–30 µg/2.4 x 109 E. coli cells 1.0 µg/mg potato
8–15 µg/40 mg lung 60–65 µg/3 x 107 S. cerevisiae cells 0.1 µg/mg Arabidopsis
15–30 µg/40 mg potato 90–100 µg/100 mg potato 0.38 µg/mg A. niger
20–30 µg/3 x 107 S. cerevisiae cells 5–10 µg/100 mg Arabidopsis 0.63 µg/2.4 x 107 E. coli cells
35–55 µg/2.4 x 109 E. coli cells 10–15 µg/50 mg A. niger 0.31 µg/3 x 105
35–55 µg/2.4 x 109 B. cereus cells S. cerevisiae cells

Purity A260 /A280 of 1.9–2.1 A260 /A280 of 1.9–2.1 A 260 /A 280 of 1.9–2.1 A 260 /A 280 of 1.9–2.1

* Removal not required.

** Total preparation time will vary depending on the tissue or cell type and on which format is used (vacuum or spin).
*** Yields will vary depending on the developmental stage, tissue type or cell line, and growth conditions used.

In the U.S., call toll free at 1-800-424-6723 Sample Preparation | 9

 Nucleic Acid Sample Preparation
RNA Isolation bio-rad.com/nasampleprep

See Also Aurum Total RNA Kits

PureZOL reagent:
page 11.
Lipid transfection
Aurum total RNA kits are designed and formulated to
reagents: assist in the isolation of highly pure and intact RNA from a
page 343. variety of starting materials. Isolated RNA is compatible with
DNA amplification/PCR:
page 355.
downstream applications including real-time qPCR, northern
blotting, microarray analysis, and cDNA library construction.
■
Isolate high yields of RNA from a wide range of sample
types in less than 60 min
■
DNase treatment ensures genomic DNA removal
■
Produce ready-to-use RNA suitable for the most
demanding downstream applications
Aurum total RNA kits offer streamlined processing using a
choice of vacuum- or spin-mediated protocols. If vacuum-
mediated purification is preferred, the protocol may be
performed with a vacuum manifold.*
For More Information Aurum Total RNA Fatty and Fibrous Tissue Kit
Web: bio-rad.com/rna-isolation
Download bulletins: 2919, 2920, and 5282

Aurum Total RNA Mini Kit Aurum Total RNA 96 Kit

The Aurum total RNA mini kit produces DNA-free total The Aurum total RNA 96 kit reproducibly isolates DNA-free
RNA from a wide range of starting materials, including total RNA from cultured cells, bacteria, and yeast in less
cultured cells, bacteria, and yeast, as well as animal and than 60 minutes in a 96-well format for high-throughput
plant tissues. The kit utilizes a stringent reagent composed total RNA isolation. The total RNA binding plate is designed
of guanidine isothiocyanate and b-mercaptoethanol for use on the Aurum vacuum manifold.*
for efficient sample lysis and quick RNase inactivation,
Consumables and Accessories
followed by purification on silica membrane in a spin-
The reagents and plasticware used in the Aurum RNA
column format using a spin- or vacuum-mediated protocol.
kits are available as refill orders. These products can be
The kit can also be used for RNA cleanup and desalting.
ordered separately from the Aurum total RNA kits.
Aurum Total RNA Fatty and Fibrous Tissue Kit
The Aurum total RNA fatty and fibrous tissue kit isolates
total RNA from samples that are difficult to disrupt. The
kit is effective for purifications involving fatty and fibrous
tissues or samples, such as fungi, that are rich in RNases.
The kit utilizes PureZOL RNA reagent, a potent phenol-
based reagent, which effectively lyses tissues and cells.
The kit includes spin columns that can be processed using
a choice of vacuum- or spin-mediated protocols.

* The Aurum vacuum manifold is no longer available. Please contact

Bio-Rad Technical Support for information on alternative products.

10 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

 Nucleic Acid Sample Preparation
bio-rad.com/nasampleprep RNA Isolation

Ordering Information
Catalog # Description Price, U.S. $
7326820 Aurum Total RNA Mini Kit, 50 preps, includes 50 RNA binding columns, 50 capless collection tubes 230.00
(2.0 ml), 100 capped sample tubes (2.0 ml), 50 capped sample tubes (1.5 ml), 1 vial lyophilized DNase I,
RNase-free reagents
7326830 Aurum Total RNA Fatty and Fibrous Tissue Kit, 50 preps, includes #7326870, 50 ml PureZOL RNA 355.00
isolation reagent
7326870* Aurum Total RNA Fatty and Fibrous Tissue Module, 50 preps, includes 50 RNA binding columns, 250.00
50 capless collection tubes (2.0 ml), 100 capped sample tubes (2.0 ml), 50 capped sample tubes (1.5 ml),
1 vial lyophilized DNase I, RNase-free reagents, and plasticware
7326800 Aurum Total RNA 96 Kit, 2 x 96-well preps, includes 2 grow blocks, sealing tape, 2 RNA binding plates, 580.00
2 collection microplates, 2 vials lyophilized DNase I, RNase-free reagents
Aurum Consumables and Accessories
7326828 DNase I, RNase-free, lyophilized, 1 vial 57.00
7326802 Aurum Total RNA Lysis Solution, RNase-free, 85 ml 52.00
7326803 Aurum Total RNA Wash High-Stringency Solution, RNase-free, 150 ml 40.00
7326804 Aurum Total RNA Wash Low-Stringency Solution, 60 ml 31.00
7326826 Aurum RNA Binding Mini Columns, 50 30.00
* Does not include PureZOL RNA isolation reagent (see catalog #7326880 or #7326890 to order separately).

PureZOL RNA Isolation Reagent See Also

Aurum total RNA kits:
page 10.
The PureZOL RNA isolation reagent protocol is an
improvement over the rapid, widely used, and proven
method of RNA isolation developed by Chomczynski and
Sacchi (1987). PureZOL RNA isolation reagent is a potent
monophasic combination of phenol and the chaotropic agent
guanidine isothiocyanate, which effectively lyses cells and
tissues, deproteinates RNA, and inactivates endogenous
nucleases in a single step. DNA and protein are efficiently
removed from the RNA following phase separation.
The ready-to-use PureZOL RNA isolation reagent is a versatile
and efficient means of isolating high yields of RNA from a DNA and protein and can be used for northern blot analysis,
variety of sources including cultured cells, animal and plant in vitro translation, poly(A)+ selection, RNase protection
tissue, yeast, virus, and bacteria samples. The single-solution assays, RT-PCR, and molecular cloning. Since no spin
format permits recovery of RNA from small quantities of columns are used in the protocol, the protocol is scalable to
tissues or cells, making it ideally suited for gene expression accommodate a wide range of sample sizes.
analysis or whenever sample quantities are limited. Total For More Information
RNA isolated using PureZOL RNA isolation reagent is free of Web: bio-rad.com/purezol

Ordering Information
Catalog # Description Price, U.S. $
7326880 PureZOL RNA Isolation Reagent, 50 ml 110.00
7326890 PureZOL RNA Isolation Reagent, 100 ml 190.00

In the U.S., call toll free at 1-800-424-6723 Sample Preparation | 11

 Nucleic Acid Sample Preparation
RNA Isolation bio-rad.com/nasampleprep

iScript RT-qPCR Sample Preparation Reagent

iScript RT-qPCR sample preparation reagent enables 104

efficient cell lysis and RNA stabilization for sensitive
quantitative gene expression analysis without RNA
purification. This unique reagent accelerates and streamlines 103
RT-qPCR analysis of cultured cells by eliminating the need to

RFU
purify RNA. Reverse transcription and real-time PCR (qPCR)
can be performed directly from cell lysates. This reagent is 102
ideal for rapid, high-throughput gene expression analysis
such as validation of siRNA-mediated gene knockdown.
101
■
Rapid protocol (5–10 min) efficiently stabilizes RNA
0 10 20 30 40
■
 ensitive detection of high-, medium-, and low-copy
S Cycles
number gene targets directly from cell lysates iScript RT-qPCR sample preparation reagent provides rapid
■
 eagent enables multiplex qPCR detection of up to 4
R yet sensitive gene expression results for high-, medium-, and
low-copy number gene targets. HeLa cells were treated with
targets from as few as 10 cells iScript RT-qPCR sample preparation reagent at 125 cells/μl and the
For More Information expression levels of three different genes were assessed. 18S rRNA
Web: bio-rad.com/iscript-sampleprep (—), b-tubulin (—), and CMYC (—) expression levels were determined
Request or download bulletin: 5736 by performing reverse transcription (cDNA synthesis kit) and qPCR
(iQ SYBR® Green supermix) directly from cell lysate preparations.
RFU, relative fluorescence units.

iScript RT-qPCR sample

Collect for 10 min preparation reagent method:
Spin
RT-qPCR 10 min

Cells Lysis

Bind to
DNase Elute for Standard method:
column >30 min
treatment RT-qPCR >30 min
matrix

Ordering Information
Catalog # Description Price, U.S. $
1708898 iScript RT-qPCR Sample Preparation Reagent, 100 reactions, 10 ml, contains RNase inhibitors and 150.00
RNA stabilizers
1708899 iScript RT-qPCR Sample Preparation Reagent, 500 reactions, 5 x 10 ml, contains RNase inhibitors and 530.00
RNA stabilizers

12 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

 Nucleic Acid Sample Preparation
bio-rad.com/nasampleprep DNA Isolation

DNA Isolation
DNA Isolation Kit Selection Guide
DNA Preparation Bacterial Culture Purification Binding Growth
Kit Yield Time Volume Format Matrix Media
Quantum Prep ≤40 µg <15 min 1–10 ml Spin Diatomaceous Enriched
plasmid miniprep earth or standard
Quantum Prep ≤400 µg 45 min 20–40 ml Spin Diatomaceous Enriched
plasmid midiprep earth or standard
Aurum ≤20 µg <10 min 1–5 ml Vacuum Silica Standard
plasmid mini and spin membrane

Quantum Prep Plasmid Kits

Quantum Prep Plasmid Miniprep Kit

The Quantum Prep miniprep kit offers high quality and Quantum Prep Plasmid Miniprep Kit

yield in an easy spin-based procedure that takes less than

15 minutes from cell culture to purified plasmid. Plasmid
DNA is recovered in water or TE for immediate use in all
downstream molecular biology applications.
Quantum Prep Plasmid Midiprep Kit
The Quantum Prep plasmid midiprep kit uses a simple
spin-column procedure that significantly reduces the time
required to generate the large quantities of plasmid DNA
needed to support applications such as transfection,
subcloning, and other enzymatic manipulations.
Quantum Prep Plasmid Midiprep Kit
For More Information
Web: bio-rad.com/quantum-prep
Download bulletin: 2325

Ordering Information
Catalog # Description Price, U.S. $
7326100  uantum Prep Plasmid Miniprep Kit, 100 preps, includes 25 ml cell resuspension solution, 25 ml cell lysis
Q 195.00
solution, 25 ml neutralization solution, 20 ml Quantum Prep matrix, 63 ml wash solution, 100 spin columns
7326120 Quantum Prep Plasmid Midiprep Kit, 20 preps, includes 110 ml cell resuspension solution, 110 ml cell lysis 190.00
solution, 110 ml neutralization solution, 20 ml Quantum Prep matrix, 125 ml wash solution, 20 midi spin columns

In the U.S., call toll free at 1-800-424-6723 Sample Preparation | 13

 Nucleic Acid Sample Preparation
DNA Isolation bio-rad.com/nasampleprep

See Also Aurum Plasmid Mini Kit

Lipid transfection
reagents:
page 343. The easy-to-use Aurum plasmid mini kit improves the
DNA amplification/PCR: efficiency and throughput of plasmid purifications with a simple
page 355.
bind-wash-elute protocol using silica membranes, all in less
than 10 minutes. Spin- and vacuum-mediated protocols are
available. The purified plasmid DNA can be immediately used
in any downstream molecular biology application.
The Aurum plasmid mini kit delivers high yields of
reproducible plasmid DNA preparations for:
■
Automated fluorescence-based sequencing
■
Restriction digestion
■
Ligation and transformation
■
Transfection
■
PCR
For More Information
Web: bio-rad.com/aurum-plasmid
Download bulletin: 2664

Ordering Information
Catalog # Description Price, U.S. $
7326400 Aurum Plasmid Mini Kit, 100 preps, includes plasmid-binding mini columns, 100 capless collection tubes, 217.40
reagents, protocol overview

See Also InstaGene Matrix

Molecular biology
and biotechnology
grade resins: InstaGene matrix is designed for rapid isolation of small
page 94. amounts of genomic DNA of sufficient purity for PCR in
less than an hour. The specially formulated 6% w/v Chelex
resin adsorbs cell lysis products that interfere with PCR,
leaving genomic DNA template in the supernatant where it
is immediately available for PCR reactions.
For More Information
Web: bio-rad.com/instagene-matrix
Download bulletin: 2074

InstaGene Protocol

PCR-ready
Add bacterial colony to water. Spin and remove supernatant. Add InstaGene matrix and incubate at 56°C for 30 min. Spin; use supernatant for PCR.
Vortex and incubate at 100°C for 8 min.

Ordering Information
Catalog # Description Price, U.S. $
7326030 InstaGene Matrix, 20 ml, sufficient for 100 extractions 54.00

14 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

 Nucleic Acid Sample Preparation
bio-rad.com/nasampleprep DNA Cleanup

Chelex 100 Molecular Biology Grade Resin See Also

Molecular biology
and biotechnology
Nuclease and ligase inhibitor-free, this pipettable, small- For More Information grade resins:
scale resin is certified not to inhibit PCR and ensures Web: bio-rad.com/dna-isolation page 94.
Download bulletin: 2074
complete removal of PCR inhibitors and metal ions.

Ordering Information
Catalog # Description Price, U.S. $
1421253 Chelex 100 Resin, molecular biology grade, 50 g, sodium, 200–400 dry mesh, 75–150 µm wet bead 195.00

DNA Cleanup
Bio-Rad offers a variety of sample preparation and cleanup columns; many are also suitable for DNA fragment purification.

Freeze ’N Squeeze DNA Gel Extraction Spin Columns See Also

ReadyAgarose
precast gel system:
Freeze ’N Squeeze spin columns offer a filtration-based page 267.
1 2 3 4 5 6 7
purification method, which provides a quick and effective DNA ladders:
page 275.
alternative to chemical extraction and electroelution methods.
The Freeze ’N Squeeze method uses centrifugation to draw
Recovery of DNA
50–23,000 bp DNA out of agarose gel slices that have been fragments using
quickly frozen and thawed. Features include: Freeze ’N Squeeze
1,000 bp spin columns.
■
No solutions to prepare — save time and avoid using Extracted fragments
700
toxic chaotropic materials 600 were rerun on a
500
■
DNA immediately available for PCR, subcloning, ligations, 400 ReadyAgarose 1% TAE
300 gel. Lane 1, precision
and sequencing reactions 200
molecular mass ruler;
100
■
Less than 1 min of hands-on time lane 7, EZ Load 100 bp
PCR molecular ruler.
For More Information
Web: bio-rad.com/freeze-n-squeeze

Freeze ’N Squeeze Method

–20ºC for 5 min

Purified DNA

Cut out gel slice. Chop into pieces. Transfer to spin column Spin for 3 min. Recover purified DNA.
and freeze for 5 min.

Ordering Information
Catalog # Description Price, U.S. $
7326165  reeze ’N Squeeze DNA Gel Extraction Spin Columns, 25 columns
F 60.00
7326166 Freeze ’N Squeeze DNA Gel Extraction Spin Columns, 100 columns 185.00

In the U.S., call toll free at 1-800-424-6723 Sample Preparation | 15

 Nucleic Acid Sample Preparation
DNA Cleanup bio-rad.com/nasampleprep

Prepacked Spin Columns

Prepacked size exclusion spin columns allow easy These columns are available in multiple sizes and offer
cleanup and purification of DNA and proteins from lower multiple MW exclusion limits to accommodate a variety
MW contaminants. Bio-Spin, Micro Bio-Spin, and of needs. Use the chart below to choose the column that
PCR Kleen columns clean up DNA or protein samples best meets your needs.
quickly and easily using size exclusion chromatography.

Prepacked Spin Column Selection Guide

Bio-Spin P-6 Micro Bio-Spin P-6 Bio-Spin P-30 Micro Bio-Spin P-30 PCR Kleen
Packed support Special grade Special grade Special grade Special grade Special grade
Bio-Gel P-6 gel Bio-Gel P-6 gel Bio-Gel P-30 gel Bio-Gel P-30 gel size exclusion gel
Equilibration buffer 10 mM Tris, pH 7.4, 10 mM Tris, pH 7.4, 10 mM Tris, pH 7.4, 10 mM Tris, pH 7.4, 10 mM Tris, 1 mM
or SSC buffer* or SSC buffer* or SSC buffer* or SSC buffer* EDTA, pH 7.0
Applications
Desalting of • • — — —
oligonucleotides
>20 bases
Labeling reactions: — — • • —
removal of unincorporated
nucleotides >20 bases
or bp from DNA
Removal of primers and — — — — •
primer-dimers from
PCR products >200 bp
Buffer exchange • • — • —
(restriction fragments,
PCR products, enzyme
reactions, sequencing
templates)
DNA sequencing — — • • —
reaction mixture cleanup**
Riboprobe cleanup*** — — — • —
Desalting of antibody, • • — • —
enzyme, and protein
solutions
Purification of proteins • • — — —
of molecular weight
>6,000
Purification of proteins — — • • —
of molecular weight
>40,000
Bed volume 1.1 ml 0.7 ml 1.1 ml 0.7 ml 0.6 ml
Retention and recovery 90% recovery of 90% recovery of 95% recovery of 95% recovery of 85% recovery of
20 bases or bp, 20 bases or bp, 22 bases or bp, 22 bases or bp, ≥700 bp, 95%
99% retention 99% retention 98% retention 98% retention retention of primers
of salts of salts of ddNTPs of ddNTPs and primer-dimers
Molecular weight 6,000 6,000 40,000 40,000 8,000,000
exclusion limit,
globular proteins
Sample volume 50–100 µl 10–75 µl 50–100 µl 10–75 µl 25–100 µl
Centrifuge type Swinging bucket Microcentrifuge Swinging bucket Microcentrifuge Microcentrifuge
Autoclavability Yes Yes Yes Yes Yes
* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0.
** In Tris buffer.
*** In RNase-free Tris buffer.

16 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

 Nucleic Acid Sample Preparation
bio-rad.com/nasampleprep DNA Cleanup

Prepacked Bio-Spin and Micro Bio-Spin Columns See Also

End cap End cap
Bio-Spin and Micro Bio-Spin columns clean up and remove Empty columns:
salts, nucleotides, dye terminators, and small molecules from Reservoir Reservoir page 140.
DNA, RNA, and protein samples in 10 minutes. Filled with Bio-Gel P resin:
3 cm page 116.
specially sized Bio-Gel P gels, these columns are shipped
Bio-Spin,
fully hydrated in Tris or SSC buffer. They yield 95% recovery 5 cm 2 cm working bed height
Micro Bio-Spin, and
of DNA >22 bp and allow sample loads of 10–100 µl. For safe 0.8 ml bed
Mini Bio-Spin
3.7 cm working bed height empty columns:
riboprobe preparation use RNase-free Micro Bio-Spin P-30 volume page 140.
Tris spin columns. 1.2 ml bed volume
For More Information Porous 30 µm
Web: bio-rad.com/dna-cleanup and /protein-cleanup polyethylene bed Luer end
support retains fitting with
snap-off tip
PCR Kleen Spin Columns fine particles

PCR Kleen columns are prepacked spin columns for Luer end fitting
purifying PCR products and other DNA molecules >200 bp with snap-off tip

directly from reaction mixtures. A simple 4-minute spin Prepacked Bio-Spin and Micro Bio-Spin Columns
effectively removes salts, nucleotides, enzymes, primers,
and primer-dimers. Purified DNA fragments are immediately
available for secondary PCR, subcloning, restriction digests,
ligations, sequencing, and other enzymatic manipulations.
For More Information
Web: bio-rad.com/dna-cleanup PCR Kleen Spin Columns
Download bulletin: 2311

Ordering Information
Catalog # Description Price, U.S. $
Micro Bio-Spin Columns with Bio-Gel P-6 in Tris Buffer
7326221 Micro Bio-Spin P-6 Columns, includes 25 columns in Tris buffer, 50 collection tubes 110.00
7326222 Micro Bio-Spin P-6 Columns, includes 100 columns in Tris buffer, 200 collection tubes 365.00
Micro Bio-Spin Columns with Bio-Gel P-30 in Tris Buffer
7326223 Micro Bio-Spin P-30 Columns, includes 25 columns in Tris buffer, 50 collection tubes 110.00
7326224 Micro Bio-Spin P-30 Columns, includes 100 columns in Tris buffer, 200 collection tubes 365.00
7326250 Micro Bio-Spin P-30 Columns, includes 25 columns in Tris buffer, 50 collection tubes, RNase-free 125.00
7326251 Micro Bio-Spin P-30 Columns, includes 100 columns in Tris buffer, 200 collection tubes, RNase-free 440.00
Micro Bio-Spin Columns with Bio-Gel P-6 in SSC Buffer
7326200 Micro Bio-Spin P-6 Columns, includes 25 columns in SSC buffer, 50 collection tubes 110.00
7326201 Micro Bio-Spin P-6 Columns, includes 100 columns in SSC buffer, 200 collection tubes 365.00
Micro Bio-Spin Columns with Bio-Gel P-30 in SSC Buffer
7326202 Micro Bio-Spin P-30 Columns, includes 25 columns in SSC buffer, 50 collection tubes 110.00
7326203 Micro Bio-Spin P-30 Columns, includes DISCONTINUED
100 columns in SSC buffer, 200 collection tubes 365.00
Bio-Spin Columns with Bio-Gel P-6 in Tris Buffer
7326227 Bio-Spin P-6 Columns, includes 25 columns in Tris buffer, 50 collection tubes 135.00
7326228 Bio-Spin P-6 Columns, includes 100 columns in Tris buffer, 200 collection tubes 455.00
Bio-Spin Columns with Bio-Gel P-30 in Tris Buffer
7326231 Bio-Spin P-30 Columns, includes 25 columns in Tris buffer, 50 collection tubes 135.00
7326232 Bio-Spin P-30 Columns, includes 100 columns in Tris buffer, 200 collection tubes 455.00
Bio-Spin Columns with Bio-Gel P-6 in SSC Buffer
7326002 Bio-Spin P-6 Columns, includes 25 columns in SSC buffer, 50 collection tubes 155.00
Bio-Spin Columns with Bio-Gel P-30 in SSC Buffer
7326006 Bio-Spin P-30 Columns, includes 25 columns in SSC buffer, 50 collection tubes 155.00
PCR Kleen Spin Columns
7326300 PCR Kleen Spin Columns, 25 78.75

In the U.S., call toll free at 1-800-424-6723 Sample Preparation | 17

Discontinued products are no longer available.
 Sample Preparation
Subject Index

Subject Index
Aurum Kits and Columns, 8
Aurum Plasmid Mini Kit, 14
Aurum Total RNA Kits, 10
Chelex 100 Molecular Biology Grade Resin, 15
Fractionation by Cellular Location, 6
Fractionation by Solubility, 5
Freeze ’N Squeeze DNA Gel Extraction Spin Columns, 15
InstaGene Matrix, 14
iScript RT-qPCR Sample Preparation Reagent, 12
Kits for Cell Lysis, 3
Prepacked Spin Columns, 16
ProteoMiner Protein Enrichment System, 7
PureZOL RNA Isolation Reagent, 11
Quantum Prep Plasmid Kits, 13
ReadyPrep Protein Extraction Kit, 3
SureBeads Magnetic Beads System, 2

18 | Sample Preparation In the U.S., call toll free at 1-800-424-6723

Crossed out products are discontinued and no longer available.
Sample Quantitation
Protein Assay Kits 20

Cuvettes 23
 Protein Assay Kits
bio-rad.com/proteinassays

Protein Assay Kits

See Also Determining the concentration of protein samples is critical for many experiments, and most protein samples can be
quantitated by a colorimetric assay. In a typical protein assay, a chemical reagent is added to a protein sample solution,
Cuvettes: page 23­.
producing a color change that is measured with a spectrophotometer or microplate reader and compared to a standard
curve of known protein concentrations. Bio-Rad offers four protein assays, each with a unique set of advantages.
All assays can be easily automated for large numbers of samples.
Bio-Rad offers four protein assays:
■
Quick Start Bradford protein assay — the single-step ■
DC protein assay — for use with samples
method for determining protein concentration in solution containing detergent
■
 io-Rad protein assay — for quantitating most proteins
B ■
RC DC protein assay — for complex sample solutions
and polypeptides with MWs >3,000 containing reducing agents and detergents
For More Information
Web: bio-rad.com/proteinassays
Download bulletins: 1069, 6835, 6836, 6837, 6838, and 6852

Protein Assay Selection Guide*

Quick Start Bradford Bio-Rad DC RC DC
Application Single-step method Quantitates most For samples with For complex samples
proteins detergent with reductants and detergents
Method adapted from Bradford (1976) Bradford (1976) Lowry et al. (1951) Lowry et al. (1951)
Standard-concentration assay
Sample volume 100 µl 100 µl 100 µl 100 µl
Linear range 0.125–1.5 mg/ml 0.125–1.5 mg/ml 0.125–1.5 mg/ml 0.2–1.5 mg/ml
Low-concentration assay
Sample volume 1 ml 800 µl 200 µl 200 µl
Linear range 1.25–25 µg/ml 1.25–25 µg/ml 5–250 µg/ml 5–250 µg/ml
Microplate assay sample volume 5 µl 10 µl 5 µl **
Minimum incubation time 5 min 5 min 15 min 15 min
Assay wavelength 595 nm 595 nm 650–750 nm 650–750 nm
* Standard-concentration assay is designed for smaller sample volumes of higher protein concentration, while the low-concentration assay is
designed for larger sample volumes of lower protein concentration.
** To adapt the RC DC assay to a microplate format, follow the micro test tube (microfuge tube) assay protocol in the RC DC protein assay instruction
manual up to the centrifugation step where the supernatant is discarded. The pellet can then be transferred to the microplate, and the microplate
assay protocol in the DC protein assay instruction manual can be followed.

Quick Start Bradford Protein Assay

The Quick Start Bradford protein assay is a streamlined,

accurate procedure for determining the concentration
of protein in solution. Ready-to-use dye reagent and
prediluted protein standards allow one-step determination
of protein concentration.
Quick Start Bradford kits offer either bovine serum albumin
or bovine g-globulin standard sets. Each kit contains two
aliquots each of seven concentrations (0.125, 0.25, 0.5, 0.75,
1.0, 1.5, and 2.0 mg/ml) conveniently packaged in screwcap
vials, eliminating ampoules and ensuring protein stability for
one year when stored at 4°C. Standards are also available in
five 2 mg/ml aliquots for creating your own dilutions.
For More Information
The 1x dye reagent can be used for performing 1 and 5 ml Web: bio-rad.com/quickstart
standard assays, microplate assays, or microassays. Download bulletins: 1069, 2969, 6835, and 6852

20 | Sample Quantitation In the U.S., call toll free at 1-800-424-6723

 Protein Assay Kits
bio-rad.com/proteinassays

Ordering Information
Catalog # Description Price, U.S. $
5000201  uick Start Bradford Protein Assay Kit 1, includes 1x dye reagent (1 L), bovine serum albumin standard
Q 150.00
(5 x 2 mg/ml); sufficient for 200 standard assays or 4,000 microplate assays
5000202 Quick Start Bradford Protein Assay Kit 2, includes 1x dye reagent (1 L), bovine serum albumin standard set 190.00
(2 sets of 7 concentration standards, 0.125–2.0 mg/ml, 2 ml)
5000204 Quick Start Bradford Protein Assay Kit 4, includes 1x dye reagent (1 L), bovine g-globulin standard set 195.00
(2 sets of 7 concentration standards, 0.125–2.0 mg/ml, 2 ml)
Reagents
5000205 Quick Start Bradford 1x Dye Reagent, 1 L 115.00
Accessories
5000206 Quick Start Bovine Serum Albumin Standard, 5 x 2 ml vials of 2 mg/ml 79.00
5000207 Quick Start Bovine Serum Albumin Standard Set, 2 sets of 7 concentration standards, 0.125–2.0 mg/ml 140.00
5000208 Quick Start Bovine g-Globulin Standard, 5 x 2 ml vials of 2 mg/ml 81.00
5000209 Quick Start Bovine g-Globulin Standard Set, 2 sets of 7 concentration standards, 0.125–2.0 mg/ml 140.00

Bio-Rad Protein Assay

The Bio-Rad protein assay is a simple colorimetric assay

for determining total protein concentration. It is easy to
adapt the assay from the standard-concentration range
to a low-concentration microassay or for use in 96-well
microplates for rapid determinations.
The Bio-Rad protein assay is based on the Bradford dye-
binding procedure (Bradford 1976), which measures the
color change of Coomassie Brilliant Blue G-250 dye when
it binds to protein (primarily to basic and aromatic amino
acid residues). The assay quantitates most proteins and
polypeptides with MWs >3,000. Some detergents and
basic buffers interfere with the assay.
For More Information
Web: bio-rad.com/BRprotein
Download bulletins: 1069, 1123, 6836, and 6852

Ordering Information
Catalog # Description Price, U.S. $
5000001  io-Rad Protein Assay Kit I, includes 450 ml dye reagent concentrate, bovine g-globulin standard;
B 150.00
sufficient for 440 standard assays or 2,200 microplate assays
5000002 Bio-Rad Protein Assay Kit II, includes 450 ml dye reagent concentrate, bovine serum albumin standard; 150.00
sufficient for 440 standard assays or 2,200 microplate assays
Reagents
5000006 Bio-Rad Protein Assay Dye Reagent Concentrate, 450 ml 120.00
Accessories
5000005 Protein Standard I, bovine g-globulin, reconstituted volume 20 ml 74.00
5000007 Protein Standard II, bovine serum albumin, reconstituted volume 20 ml 74.00

In the U.S., call toll free at 1-800-424-6723 Sample Quantitation | 21

 Protein Assay Kits
bio-rad.com/proteinassays

DC Protein Assay

The DC (detergent compatible) protein assay is a colorimetric

assay for protein determination of samples that contain
detergents. The reaction is similar to the well-documented
Lowry assay (Lowry et al. 1951), but has been modified
to save time. The DC protein assay requires only a single
15 minute incubation, and the absorbance readings are
stable for at least 2 hours.
For More Information
Web: bio-rad.com/DCprotein
Download bulletins: 1069, 1731, 1770, 1909, 6837, and 6852

Ordering Information
Catalog # Description Price, U.S. $
5000111  C Protein Assay Kit I, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin reagent,
D 190.00
5 ml surfactant solution, bovine g-globulin standard; sufficient for 450 standard assays
5000112 DC Protein Assay Kit II, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin reagent, 175.00
5 ml surfactant solution, bovine serum albumin standard; sufficient for 450 standard assays
Reagents
5000113 Protein Assay Reagent A, 250 ml, alkaline copper tartrate solution 55.00
5000114 Protein Assay Reagent B, 1 L, dilute Folin reagent 55.00
5000115 Protein Assay Reagent S, 5 ml, surfactant solution 37.00
5000116 DC Protein Assay Reagents Package, includes 250 ml alkaline copper tartrate solution, 180.00
2 L dilute Folin reagent, 5 ml surfactant solution; sufficient for 450 standard assays
Accessories
5000005 Protein Standard I, bovine g-globulin, lyophilized (reconstituted volume 20 ml) 74.00
5000007 Protein Standard II, bovine serum albumin, lyophilized (reconstituted volume 20 ml) 74.00

See Also RC DC Protein Assay

Protein sample
preparation:
page 2. The RC DC (reducing agent and detergent compatible)
Cuvettes: page 23. protein assay is a colorimetric assay for protein
determination in the presence of reducing agents and
detergents. The RC DC protein assay, based on the
Lowry protocol (Lowry et al. 1951), includes the features
of the original DC protein assay. Its compatibility with
a broader range of reagents allows simplified protein
quantitation directly in complex sample solutions.
For More Information
Web: bio-rad.com/RCDCprotein
Download bulletins: 1069, 2610, 6838, and 6852

22 | Sample Quantitation In the U.S., call toll free at 1-800-424-6723

 Cuvettes
bio-rad.com/assaycuvettes

Ordering Information
Catalog # Description Price, U.S. $
5000121  C DC Protein Assay Kit I, includes RC reagents package, DC protein assay reagents package,
R 295.00
bovine g-globulin standard; sufficient for 450 standard assays
5000122  C DC Protein Assay Kit II, includes RC reagents package, DC protein assay reagents package,
R 295.00
bovine serum albumin standard; sufficient for 450 standard assays
Reagents
5000113 Protein Assay Reagent A, 250 ml, alkaline copper tartrate solution 55.00
5000114 Protein Assay Reagent B, 1 L, dilute Folin reagent 55.00
5000115 Protein Assay Reagent S, 5 ml, surfactant solution 37.00
5000116 DC Protein Assay Reagents Package, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin 180.00
reagent, 5 ml surfactant solution; sufficient for 450 standard assays
5000120 RC DC Protein Assay Reagents Package, includes RC reagents package and DC protein assay reagents 290.00
package; sufficient for 450 standard assays
5000119 RC Reagents Package, includes RC reagent I (250 ml) and RC reagent II (250 ml); sufficient for 115.00
500 standard assays
Accessories
5000005 Protein Standard I, bovine g-globulin, lyophilized (reconstituted volume 20 ml) 74.00
5000007 Protein Standard II, bovine serum albumin, lyophilized (reconstituted volume 20 ml) 74.00

Cuvettes
Bio-Rad offers quartz and plastic cuvettes in a range of working volumes. See Also
For More Information Protein assay
Web: bio-rad.com/assaycuvettes kits: page 20.

Standard and Semimicrovolume Cuvettes

Polystyrene cuvettes are ideal for use with protein assays

(pages 20–22). They adsorb less Coomassie Blue
dye than glass or quartz cuvettes. Assays can be mixed
directly in the standard 3.5 ml cuvettes, and the volume
of reagents can be reduced, yielding more assays per kit.
Semimicrovolume 1.5 ml cuvettes are ideal for precise
quantitation of small-volume samples. Both standard
and semimicrovolume cuvettes are sized to fit most
spectrophotometers. Bio-Rad cuvettes offer smooth
optical surfaces for consistent and accurate readings.
Bio-Rad also offers other cuvettes for specialized
applications, including quartz and disposable cuvettes.
For More Information
Web: bio-rad.com/assaycuvettes

Cuvette Racks

In the U.S., call toll free at 1-800-424-6723 Sample Quantitation | 23

 Cuvettes
bio-rad.com/assaycuvettes

Ordering Information
Catalog # Description Price, U.S. $
Disposable Polystyrene Cuvettes
2239950 Standard Disposable Polystyrene Cuvettes, 3.5 ml, 100 12.50
2239955 Semimicrovolume Disposable Polystyrene Cuvettes, 1.5 ml, 100 12.50
Quartz Cuvettes
1702502 Standard Cuvette, 1–3.5 ml, 10 mm path length, quartz 177.50
1702503 Semimicrovolume Cuvette, 0.5–1.4 ml, 10DISCONTINUED
mm path length, quartz 266.25
1702504 Microvolume Cuvette, 200–700 µl, 10 mm path length, quartz 273.75
1702505 Submicrovolume Cuvette, 80–100 µl, 10 mm path length, quartz 548.75
VersaFluor Disposable Cuvettes and Cuvette Racks
1702415 Standard Cuvettes, 12.5 x 12.5 mm (outside dimensions), 4-sided, optically clear polystyrene, 3.5 ml, 100 25.75
1702416
DISCONTINUED
Microcuvettes, 3.0 x 3.0 mm (outside dimensions), 4-sided, optically clear polystyrene, 150–350 µl, 100 46.35
1660485 Cuvette Racks, hold 12 cuvettes each, set of 5 73.75

24 | Sample Quantitation In the U.S., call toll free at 1-800-424-6723

Discontinued products are no longer available.
 Sample Quantitation
Subject Index

Subject Index
Bio-Rad Protein Assay, 21
DC Protein Assay, 22
Quick Start Bradford Protein Assay, 20
RC DC Protein Assay, 22
Standard and Semimicrovolume Cuvettes, 23

In the U.S., call toll free at 1-800-424-6723 Sample Quantitation | 25

 This page intentionally left blank.

26 | Sample Quantitation In the U.S., call toll free at 1-800-424-6723

Cell Counting
Automated Cell Counting System 28
 Automated Cell Counting System
bio-rad.com/cellcount

Automated Cell Counting System

Video TC20 Automated Cell Counter
Product
Demonstration
With its innovative auto-focus and sophisticated cell
counting algorithm, the TC20 automated cell counter
eliminates subjectivity while delivering reliable counts
of mammalian cells in 30 seconds.
bio-rad.com/ ■
 ompatible with a broad range of cell sizes and types —
C
TC20start
counts cell lines, primary cells (from tissue or blood),
and stem cells
■
Innovative auto-focus technology — removes the
variation associated with manual focusing and leads to
precise cell counts in 30 sec
■
 ell size gates — user selects a population of interest in
C
complex samples, such as primary cells, or lets the cell
counting algorithm do all the work
■
 ell viability — analyzes cells accurately using multifocal
C
plane analysis
■
 asy to archive and analyze — stores up to 100 counts
E
in the onboard memory for access any time, or use the
optional TC20 data analyzer software on your PC to
further analyze exported cell images
Upon insertion of a counting slide, the TC20 provides
a total cell count with or without staining and assesses
cell viability via trypan blue exclusion. To determine if a cell
is viable, the TC20 counter analyzes each cell on images
acquired from multiple focal planes during the focusing
step. Auto-focus and multifocal plane analysis reduce
counting bias associated with manual focusing.
Accuracy is comparable to results obtained with a
hemocytometer. The TC20 counter can count cells with
a 6–50 μm cell diameter and within a broad concentration
range of 5 x 104–1 x 107 cells/ml, which eliminates the
need to dilute cells, thus reducing the errors associated TC20 Automated Cell Counter, Counting Slides, and Trypan Blue

with sample dilutions prior to counting.

For complex samples composed of multiple cell
populations, such as primary cells, the position of cell size
gates can be adjusted to define the population of interest.
Results from 100 previous counts are stored in the TC20
cell counter and can be exported via the USB port.
Images of cells can be further analyzed on a computer
using the TC20 data analyzer software.
For More Information
Web: bio-rad.com/TC20
Request or download bulletins: 6282 and 6283

Learn More about the Technology

Web: bio-rad.com/tech/cellcounting

28 | Cell Counting In the U.S., call toll free at 1-800-424-6723

 Automated Cell Counting System
bio-rad.com/cellcount

Ordering Information
Catalog # Description Price, U.S. $
TC20 Automated Cell Counter
1450102 TC20 Automated Cell Counter, 120–240 V, includes instrument, power supply, USB flash drive, 4,220.00
30 dual-chamber counting slides (60 counts), 1.5 ml trypan blue
1450103 TC20 Automated Cell Counter with Thermal Label Printer, 120–240 V, includes instrument, 4,750.00
power supply, USB flash drive, USB cable, thermal label printer, 1 roll of 500 labels, 30 dual-chamber
counting slides (60 counts), 1.5 ml trypan blue
Kits and Reagents*
1450003 Counting Kit, includes 30 dual-chamber counting slides (60 counts), 1.5 ml trypan blue 53.00
1450013 Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution, 9.00
sterile filtered, sufficient for 150 counts (10 µl/count), 1 x 1.5 ml
1450021 Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution, 44.00
sterile filtered, sufficient for 750 counts (10 µl/count), 5 x 1.5 ml
1450022 Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution, 82.00
sterile filtered, sufficient for 1,500 counts (10 µl/count), 10 x 1.5 ml
1450015 Counting Slides, 150 dual-chamber counting slides (300 counts) 270.00
1450016 Counting Slides, 300 dual-chamber counting slides (600 counts) 525.00
1450017 Counting Slides, 600 dual-chamber counting slides (1,200 counts) 1,000.00
1450018 Counting Slides, 900 dual-chamber counting slides (1,800 counts) 1,420.00
1450019 Counting Slides, 1,200 dual-chamber counting slides (2,400 counts) 1,760.00
1450020 Counting Slides, 2,400 dual-chamber counting slides (4,800 counts) 3,300.00
1450014 System Test Kit, includes verification slide, instructions 930.00
Accessories*
1450005 Thermal Label Printer, 120–240 V, includes thermal label printer, USB cable, 1 roll of 500 labels 700.00
1450007 Thermal Printer Labels, 1 roll of 500 labels, for thermal label printer 35.00
* Compatible with TC20 and TC10 automated cell counters.

In the U.S., call toll free at 1-800-424-6723 Cell Counting | 29

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30 | Cell Counting In the U.S., call toll free at 1-800-424-6723

Flow Cytometry
Cell Sorting 32
Instruments 32
Consumables 37
Reagents 40

Cell Analysis 41
Instruments 41
Instrument Upgrades 44
Reagents 47

Cell Biology Reagents 48

Antibody Labeling Kits 48
Cell Proliferation Assays 52
Cell Viability Assays 56
Flow Cytometry Calibration and Validation Reagents 59
Flow Cytometry General Reagents 61
Nuclear Staining Dyes 63

Regulated Cell Death Reagents 65

Apoptosis Reagents 65
Autophagy Reagents 71
Pyroptosis Reagents 73
 Cell Sorting
Instruments bio-rad.com/cellsorting

Cell Sorting
Cell sorting provides a method, typically using fluorescence, to sort or isolate a homogenous population of cells from a
heterogeneous mixture of cells based on intracellular and/or extracellular properties. Expressed fluorescent proteins, such
as green fluorescent protein (GFP), or fluorophores conjugated to antibodies bind to markers of interest, which allows for a
starting population to be sorted to greater than 99% purity. While cell sorting is commonly used in fields such as immunology,
cancer biology, and stem cell biology, it is quickly becoming a useful tool in broader research studies from microbiology to
neuroscience. Cell sorting can also be used as an upstream tool for proteomic or genomic experiments to achieve more
specific results by reducing background noise or false positives.

Instruments

See Also S3e Cell Sorter

S3e Biosafety
System Class I:
page 35. The S3e cell sorter is the first truly walk-away automated
Cell sorting cell sorter available to scientists. The S3e is a compact
consumables: cell sorter equipped with one, two, or three lasers and
page 37.
Cell biology
up to four fluorescence detectors plus forward and side
reagents: scatter detection. The cell sorter utilizes established jet-in-
page 48.
air technology in which cells are analyzed directly within
Cell counting:
page 27.
the stream before being sorted. Samples can be sorted at
high speeds while sensitivity and purity are maintained.

Videos With its unique feature set and technical ease of use, the
automated S3e cell sorter allows you to perform one to
Product
Technology four color sorts in less than 30 minutes in your laboratory
and greatly enhances your experimental success.
■
 tilizes stream-in-air fluid dynamics for a more gentle
U
high-speed sort
bio-rad.com/
■
 eatures Lossless Cell Sorting mode for maximum
F
S3eDevelopment recovery of target cell populations from your precious
Customer samples
Testimonial
■
 as simplified instrument setup and the intuitive software
H
ensures user success, regardless of experience level
■
 eatures a compact design (W x D x H: 70 x 65 x 65 cm)
F
that includes onboard fluidics and temperature control
bio-rad.com/ systems
S3eCancerResearch
Product
■
 rovides sort status email and text notifications, which
P
Demonstration allow the user to multitask during a sort
For More Information
Web: bio-rad.com/cellsorter
Download bulletin: #10032007
Request or download bulletin: 6614
bio-rad.com/
SimplifiedSorting

32 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Sorting
bio-rad.com/cellsorting Instruments

S3e Cell Sorter System See Also

Features ProLine rainbow
Performance Droplet frequency 37–43 kHz beads: page 37.
Sorting type True jet-in-air for high-performance sorting ProFlow sort grade
8x sheath fluid:
Sorting rate No hardware limitations for sort rate, limited only by droplet frequency and application page 38.
Sorting purity >99% pure S3e fluidic container:
Sensitivity <125 MESF for FITC and PE page 39.
Temperature control Sample and collection temperature control from 4–37°C using Peltier solid-state system
Lasers/Optics One-laser system Primary: 488 nm 100 mW
Two-laser system Primary: 488 nm 100 mW
Secondary: 405 nm 100 mW, 561 nm 100 mW, or 640 nm 100 mW
Three-laser system* Primary: 405 nm 100 mW or 488 nm 100 mW
Secondary: 561 nm 100 mW or 640 nm 100 mW
Tertiary: 561 nm 100 mW or 640 nm 100 mW
Detectors Forward scatter (FSC) with photomultiplier tube (PMT)
Side scatter (SSC) with PMT
2 fluorescence detectors with PMT (one laser)
4 fluorescence detectors with PMT (two lasers)
Filters (488 laser) Long pass: 495DLP, 560DLP, 540LP
Band pass: 488/6, 525/30
Filters (488/561 lasers) Long pass: 495DLP, 560DLP, 593DLP, 655LP
Band pass: 488/6, 525/30, 615/25, 586/25
Filters (488/640 lasers) Long pass: 495DLP, 560DLP, 640DLP, 700LP
Band pass: 488/6, 526/48, 593/40, 670/30, 700LP
Filters (405/488/561 lasers) Long pass: 470DSP, 495DLP, 560LP, 593LP, 600LP
Band pass: 447/60, 488/6, 525/30, 586/25
Filters (405/488/640 lasers) Long pass: 475DSP, 495DLP, 560DLP, 640LP, 650LP
Band pass: 447/60, 488/6, 526/48, 593/40
Filters (488/561/640 lasers) Long pass: 495DLP, 560DLP, 593DLP, 640DLP, 655LP
Band pass: 488/6, 525/30, 586/25, 615/25
Fluidics Nozzle 100 μm fixed
Pressure 30 psi fixed
Sheath Onboard dilution of 8x sheath fluid or direct use of 1x sheath fluid
Minimum sample loading 200 μl
Sorting Directions Two way
Mode Single, purity, enrichment
Collection Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Physical Dimensions (W x D x H) 70 x 65 x 65 cm (instrument only)
Specifications Weight 90 kg (instrument only)
* Note that 405/561/640 nm option is not available.

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 33

 Cell Sorting
Instruments bio-rad.com/cellsorting

Ordering Information
Catalog # Description Price, U.S. $
S3e Cell Sorter
1451005 S3e Cell Sorter (488 nm), 488 nm 100 mW laser, includes 2 fluorescence detectors with filters, Inquire
AutoGimbal system, ProSort software
1451006 S3e Cell Sorter (488/561 nm), 488 and 561 nm 100 mW lasers, includes 4 fluorescence detectors with Inquire
filters, AutoGimbal system, ProSort software
1451008 S3e Cell Sorter (488/640 nm), 488 and 640 nm 100 mW lasers, includes 4 fluorescence Inquire
detectors with filters, AutoGimbal system, ProSort software
12007058 S3e Cell Sorter (405/488/561 nm), 405, 488, and 561 nm 100 mW lasers, includes 4 fluorescence
detectors with filters, AutoGimbal system, ProSort software
12007059 S3e Cell Sorter (405/488/640 nm), 405, 488, and 640 nm 100 mW lasers, includes 4 fluorescence
detectors with filters, AutoGimbal system, ProSort software
12007052 S3e Cell Sorter (488/561/640 nm), 488, 561, and 640 nm 100 mW lasers, includes 4 fluorescence
detectors with filters, AutoGimbal system, ProSort software
1451078 S3e Biosafety System Class I Inquire
1451029 S3e Cell Sorter (488 nm) with S3e Biosafety System Class I Inquire
1451030 S3e Cell Sorter (488/561 nm) with S3e Biosafety System Class I Inquire
1451032 S3e Cell Sorter (488/640 nm) with S3e Biosafety System Class I
17003806 S3e Cell Sorter (405/488/561 nm) with S3e Biosafety System Class I
17003807 S3e Cell Sorter (405/488/640 nm) with S3e Biosafety System Class I
17003808 S3e Cell Sorter (488/561/640 nm) with S3e Biosafety System Class I
S3e Cell Sorter Laser Upgrade Package
12007053 405 nm Laser Upgrade for S3e Cell Sorter (488/561 nm) 250.00
12007054 640 nm Laser Upgrade for S3e Cell Sorter (488/561 nm) 250.00
12007055 405 nm Laser Upgrade for S3e Cell Sorter (488/640 nm) 250.00
12007056 561 nm Laser Upgrade for S3e Cell Sorter (488/640 nm) 850.00
Accessories
1441001 S3e Sheath Cap Assembly, white 250.00
1441002 S3e DI Water Cap Assembly, blue 250.00
1441003 S3e Waste Cap Assembly, red 250.00
1441005 S3e Filter Block A, empty with 2 dichroic filter holders 850.00
1441006 S3e Filter Block B, empty with 2 dichroic filter holders 850.00
1441007 S3e Individual Filter Holder, black, empty 100.00
1441008 Neutral Density Filter, optical density 1.0 80.00
1441009 S3e Nozzle Tip, 100 µm 600.00
1441010 S3e Nozzle O-Ring with Alignment Disk, pkg of 2 58.00
1441011 S3e Collection Adaptor Set 290.00
1441012 Hex Driver, 2 mm 9.00
1441013 S3e Individual Dichroic Filter Holder, empty 100.00
1451076 S3e Power Conditioner, international 4,000.00
1451077 S3e Power Conditioner, U.S. 3,800.00
1451065 S3e Cell Sorter Accessory Kit, includes 100 μm nozzle tip, 2 nozzle O-rings, 2 nozzle alignment 1,000.00
disks, 1 ml syringe, 2 neutral density filters (1.0), 2 mm hex driver, spanner wrench
1451086 ProLine Universal Calibration Beads, includes 3 x 5 ml bottles of ready-to-use beads for alignment 490.00
verification and drop delay determination on the S3e cell sorter
12012932 ProFlow Sort Grade 8x Sheath Fluid, includes 5 x 4 L ready-to-use, sterile, preservative-free 490.00
phosphate buffered saline (PBS) solutions in gamma-irradiated S3e fluidic containers
1451083  roFlow Sort Grade Water, includes 5 x 4 L sterile, endotoxin-free water in gamma-irradiated
P 390.00
S3e fluidic containers
1451084 S3e Fluidic Container, 3 x 4 L gamma-irradiated sterile containers for use on the S3 or 78.00
S3e cell sorter
1451085 ProLine Rainbow Beads, includes a 5 ml bottle containing a mixture of beads dyed with 8 different 460.00
fluorescence intensities for excitation at wavelengths between 365–650 nm

34 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Sorting
bio-rad.com/cellsorting Instruments

S3e Biosafety System Class I

The S3e Biosafety System Class I is an aerosol containment

hood custom designed for the S3 or S3e cell sorter, which
provides users and the environment protection from
aerosols created during the cell sorting process. It is an
affordable alternative to traditional large and expensive
biosafety hoods commonly offered for cell sorters.
The S3e Biosafety System Class I sits directly on the
benchtop and contains the compact S3 or S3e cell sorter.
Its easy-to-open, magnetically attached vinyl walls provide
ready access to all sides of the cell sorter during operation,
cleaning and maintenance, or service.
Fully integrated with and monitored by the S3 or S3e cell
sorter’s ProSort software, users have real-time information
about HEPA filter life and the temperature of the system
inside. Fan speed is determined based on the cell sorter
■
 oftware controlled — full integration with ProSort
S
operation mode — sorting or idle. The HEPA filter provides software for fan speed regulation and temperature
protection at 99.997% for 0.3 µm particles. The system is monitoring in real time
able to perform a 100% air exchange six to eight times per ■
 ully accessible — easily opened vinyl walls are
F
minute around the S3 or S3e cell sorter. magnetically attached, allowing access to all 4 sides
of the cell sorter for cleaning and maintenance
The system adheres to the biosafety requirements of the
International Society for Advancement of Cytometry (ISAC)
■
 uiet and energy efficient — generates low
Q
for aerosol containment. vibration and sound pressure levels; specialized fans
and low backpressure from the HEPA filter reduce
Product features include: energy consumption
■
 ompact and affordable — small footprint for an
C For More Information
economical alternative to large and expensive traditional Web: bio-rad.com/S3eBiosafetyMore
biosafety hoods Download bulletin: 6404
Request or download bulletin: 6503

Ordering Information
Catalog # Description Price, U.S. $
1451005 S3e Cell Sorter (488 nm), 488 nm 100 mW laser, includes 2 fluorescence detectors with filters, Inquire
AutoGimbal system, ProSort software
1451006 S3e Cell Sorter (488/561 nm), 488 and 561 nm 100 mW lasers, includes 4 fluorescence detectors with Inquire
filters, AutoGimbal system, ProSort software
1451008 S3e Cell Sorter (488/640 nm), 488 and 640 nm 100 mW lasers, includes 4 fluorescence Inquire
detectors with filters, AutoGimbal system, ProSort software
12007058 S3e Cell Sorter (405/488/561 nm), 405, 488, and 561 nm 100 mW lasers, includes 4
fluorescence detectors with filters, AutoGimbal system, ProSort software
12007059 S3e Cell Sorter (405/488/640 nm), 405, 488, and 640 nm 100 mW lasers, includes 4
fluorescence detectors with filters, AutoGimbal system, ProSort software
12007052 S3e Cell Sorter (488/561/640 nm), 488, 561, and 640 nm 100 mW lasers, includes 4
fluorescence detectors with filters, AutoGimbal system, ProSort software
1451078 S3e Biosafety System Class I Inquire
1451029 S3e Cell Sorter (488 nm) with S3e Biosafety System Class I Inquire
1451030 S3e Cell Sorter (488/561 nm) with S3e Biosafety System Class I Inquire
1451032 S3e Cell Sorter (488/640 nm) with S3e Biosafety System Class I Inquire
17003806 S3e Cell Sorter (405/488/561 nm) with S3e Biosafety System Class I
17003807 S3e Cell Sorter (405/488/640 nm) with S3e Biosafety System Class I
17003808 S3e Cell Sorter (488/561/640 nm) with S3e Biosafety System Class I
1451076 S3e Power Conditioner, international 4,000.00
1451077 S3e Power Conditioner, U.S. 3,800.00

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 35

 Cell Sorting
Instruments bio-rad.com/cellsorting

S3e Cell Sorter IQ/OQ Services

Installation qualification (IQ) and operational qualification (OQ) services for the S3e cell sorter ensure that your system
functions properly and aids in regulatory compliance. Both services are completed by a certified service engineer.
IQ services include a careful inspection and documentation of instrument installation. The engineer ensures that the
instrument is properly installed, inspects vital system components, and documents details about the computer software
and vital system components.
OQ services verify and document that the instrument is performing according to the manufacturer’s specifications. The
thorough evaluation includes documentation of essential system information, evaluation of all key system components,
complete instrument verification, service system testing, manual and bead-based testing, and signed documentation of
the completion of the IQ/OQ services.
■
 aximize instrument uptime — planned instrument qualification services ensure your instrument is performing
M
according to specifications
■
Increase your confidence — frequent instrument validation helps minimize loss of precious samples and valuable time
associated with a malfunctioning instrument
■
Remain compliant — official documentation can aid in meeting regulatory requirements
For More Information
Web: bio-rad.com/flowiqoq

Ordering Information
Catalog # Description Price, U.S. $
12005873 S3e Cell Sorter IQ/OQ Kit, includes IQ and OQ protocols, test reagents, and accessories 390.00

36 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Sorting
bio-rad.com/cellsorting Consumables

Consumables

ProLine Universal Calibration Beads

ProLine universal calibration beads are designed to verify

the alignment performance and determine the drop delay
on any S3 or S3e cell sorter. Each bottle contains a single
population of ready-to-use fluorescent beads for a total of
30 quality control (QC) tests.
Product features include:
■
Ready-to-use fluorescent beads do not require dilution
■
 lignment and drop delay can be performed with
A
1 reagent in less than 7 min
■
Pass/fail performance result with ProSort software
■
 rending QC information in ProSort software for tracking
T
the performance of your S3 or S3e cell sorter
For More Information
Web: bio-rad.com/prolineuniversal
Download bulletin: #10041517
Request or download bulletin: 6614

Ordering Information
Catalog # Description Price, U.S. $
1451086 ProLine Universal Calibration Beads, 15 ml (3 x 5 ml) 490.00

ProLine Rainbow Beads See Also

ProFlow sort grade
8x sheath fluid:
ProLine rainbow beads are designed for instrument page 38.
performance monitoring on a flow cytometer system. ProFlow sort
Each bottle contains 3.0–3.4 µm beads dyed with eight grade water:
page 39.
different fluorescent intensities for excitation by lasers
S3e fluidic container:
between 365 and 650 nm. page 39.

■
 asily check the sensitivity and linearity of your flow
E
cytometer with one set of beads
■
 rack fluorescent fluctuations with validated bead lots
T
to ensure accurate baselines
■
 se with either the S3e cell sorter or the ZE5
U
cell analyzer
For More Information
Web: bio-rad.com/prolinerainbowbeads
Download bulletin: #10033040

Ordering Information
Catalog # Description Price, U.S. $
1451085 ProLine Rainbow Beads, includes a 5 ml bottle containing a mixture of beads dyed with 460.00
8 different fluorescent intensities for excitation at wavelengths 365–650 nm

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 37

 Cell Sorting
Consumables bio-rad.com/cellsorting

See Also ProFlow Sort Grade 8x Sheath Fluid

ProLine universal
calibration beads:
page 37. ProFlow sort grade 8x sheath fluid is a ready-to-use,
ProLine rainbow sterile, endotoxin- and preservative-free phosphate
beads: page 37. buffered saline solution. Each lot is tested for cytotoxicity
ProFlow sort
grade water:
and particulates and is bottled in gamma-irradiated
page 39. containers to ensure sterility. Up to 5 weeks of run time
S3e fluidic container: is available per case when it is used on the S3 or S3e
page 39.
cell sorter. The S3 or S3e cell sorter employs its internal
fluidics system to automatically dilute ProFlow sort grade
8x sheath fluid and creates 32 L of 1x sheath solution from
each container, generating 160 L per case.
Product features include:
■
 o manual dilution required — the S3 and S3e cell
N
sorters’ unique internal fluidics chamber automatically
generates a 1x solution, ensuring accurate dilution and
minimizing potential contamination
■
 asy container swapping — prepackaged, sterile S3e
E
fluidic container bypasses the need to fill a new container
during a sort, minimizing the risk of contamination
■
 inimum storage requirements — one 20 L case
M
creates 160 L of 1x sheath solution, freeing your storage
space for other purposes
For More Information
Web: bio-rad.com/sheath
Download bulletin: #10030825
Request or download bulletin: 6614

Ordering Information
Catalog # Description Price, U.S. $
12012932 ProFlow Sort Grade 8x Sheath Fluid, 20 L (5 x 4 L) 490.00

38 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Sorting
bio-rad.com/cellsorting Consumables

ProFlow Sort Grade Water See Also

ProLine universal
calibration beads:
This sterile, endotoxin-free solution is ideal for flow page 37.
cytometry instrumentation. ProFlow sort grade water is ProLine rainbow
beads: page 37.
designed to provide the best sort results when combined
ProFlow sort grade
with preservative-free ProFlow sort grade 8x sheath fluid 8x sheath fluid:
on any S3 or S3e cell sorter. It may also be used on the page 38.
ZE5 cell analyzer. Each lot is prepared by reverse osmosis,
passed through fine carbon, deionized through two resin
beds, serially filtered twice through 0.1 μm positively
charged membranes, and poured into gamma-irradiated
S3e fluidic containers.
For More Information
Web: bio-rad.com/proflowsortgradewater
Download bulletin: #10032248

Ordering Information
Catalog # Description Price, U.S. $
1451083  roFlow Sort Grade Water, includes 5 x 4 L sterile, endotoxin-free water in gamma-irradiated
P 390.00
S3e fluidic containers

S3e Fluidic Container

S3e fluidic containers are specifically designed for use

on the S3 or S3e cell sorter. Easily store your solutions
in the S3e fluidic containers for quick container swaps
during a run. Each 4 L container is gamma irradiated
to ensure sterility.
For More Information
Web: bio-rad.com/s3emore
Request or download bulletin: 6614

Ordering Information
Catalog # Description Price, U.S. $
1451084 S3e Fluidic Container, 3 x 4 L gamma-irradiated sterile containers for use on the 78.00
S3 or S3e cell sorter

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 39

 Cell Sorting
Reagents bio-rad.com/cellsorting

Reagents

See Also Kits and Reagents for Cell Sorting

Cell biology reagents:
page 48.
Bio-Rad offers a variety of easy-to-use kits and reagents suitable for use with the S3e cell sorter.

Ordering Information
Maximum Maximum Optimal
Excitation, Emission, Excitation S3e Cell Sorter
Catalog # Description nm nm Laser, nm Configuration Price, U.S. $
Antibody Labeling Kits
1351011 ReadiLink 405/454 403 454 405 405 laser (447/60)
1351002 ReadiLink 492/516 492 516 488 488 laser FL1 (526/48) 405.00
488 laser FL1 (525/30)
1351003 ReadiLink 555/570 555 570 561 561 laser FL2 (586/25) 405.00
1351004 ReadiLink 594/610 594 610 561 561 laser FL3 (615/25) 405.00
1351005 ReadiLink 633/655 633 655 640 640 laser FL3 (670/30) 405.00
1351006 ReadiLink 647/674 647 674 640 640 laser FL3 (670/30)
1351007 ReadiLink 680/701 680 701 640 640 laser FL4 (700/LP) 405.00
1351008 ReadiLink 700/713 700 713 640 640 laser FL4 (700/LP) 405.00
Cell Proliferation
1351202 CytoTrack Blue 403/454 403 454 405 405 laser (447/60) 38.00
1351203 CytoTrack Green 511/525 511 525 488 488 laser FL1 (525/30) 38.00
488 laser FL1 (526/48)
1351204 CytoTrack Yellow 542/556 542 556 532 488 laser FL2 (593/40) 38.00
1351205 CytoTrack Red 628/643 628 643 633 or 640 640 laser FL3 (640/30)
1351201 CFDA-SE Cell Proliferation 492 517 488 488 laser FL1 (525/30) 38.00
Assay 488 laser FL1 (525/30)
Cell Viability
1351112 VivaFix 410/450 410 450 405 405 laser (447/60)
1351113 VivaFix 408/512 408 512 405 405 laser (495 DLP, 525/30, 526/48)
1351114 VivaFix 398/550 398 550 405 405 laser (495 DLP)
1351115 VivaFix 498/521 498 521 488 488 laser FL1 (525/30)
1351116 VivaFix 547/573 547 573 561 561 laser FL2 (586/25)
1351117 VivaFix 583/603 583 603 561 561 laser FL3 (615/25)
1351118 VivaFix 649/660 649 660 640 640 laser FL3 (670/30)
1351101 Propidium Iodide 538 617 561 or 488 488 laser FL3 (615/25)
488 laser FL2 (540/LP)
488 laser FL2 (593/40)
561 laser FL3 (615/25)
1351102 7-AAD 548 648 561 or 488 488 laser FL4 (655/LP)
488 laser FL2 (540/LP)
488 laser FL3 (670/30)
561 laser FL4 (655/LP)

40 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Analysis
bio-rad.com/ZE5 Instruments

Cell Analysis
Cell analysis via flow cytometry examines the physical and chemical characteristics of particles in a fluid as it passes See Also
through at least one laser. Cell components are usually fluorescently labeled and then excited by the laser to emit light at Cell biology
varying wavelengths. Flow cytometry allows fast analysis at the single-cell level. Typical parameters include cell volume reagents:
page 48.
(forward scatter), morphological complexity (side scatter), pigments, DNA content, protein expression, cell surface and
intracellular antigens, cytokines, apoptosis, cell viability, cell monitoring, and fluorescent proteins. Life science disciplines
that utilize flow cytometry include immunology, cell biology, cancer research, plant biology, microbiology, pathology,
marine biology, and medicine.

Instruments

ZE5 Cell Analyzer

The ZE5 cell analyzer was created by gathering input

from some of the leading flow cytometer users across the
world and was designed to address the gaps in currently
available systems.
It is an innovative flow cytometer with flexible configurations
designed to meet a broad range of experimental
complexities and throughput needs. With up to 30
detectors (27 colors), the ZE5 cell analyzer is a truly
expandable flow cytometer. The fully integrated sample
loader, with the ability to handle tubes or plates without
changing instrument hardware, enables true walk-away
functionality. Features such as the stat tube station, the fluid
and reagent hot swap capability, and the automated startup
and QC allow for continuous operation and maximize
instrument running time for any busy core lab. With its API,
the ZE5 cell analyzer can be integrated into any workcell for
automated analysis. Its innovative sample delivery system
ensures quick processing, reduced carryover, and minimal
sample loss by returning unused precious cells to the
sample plate or tube for later use.
Features include:
■
Flexible loading options
■
Best-in-class and scalable optics
■
Integrated and easy-to-use fluidics
■
Innovative electronics providing highest quality data
■
High-throughput production
■
Automation/robotics capability

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 41

 Cell Analysis
Instruments bio-rad.com/ZE5

Specifications
System Fluorescence sensitivity <100 MESF for FITC, PE, APC

Scatter sensitivity <0.3 µm FSC resolution with small particle detection module

Loader Integrated sample loader with agitation and temperature control. Up to 384-well plates;
40-tube rack for 5 ml 12 x 75 mm tube loading. Stat tube position for single tube loading
Optics Excitation Up to five spatially separated lasers per instrument. Standard options include:
355 nm, 50 mW 405 nm, 100 mW 561 nm, 50 mW
488 nm, 100 mW 640 nm, 100 mW

Detection Up to 30 detectors including FSC and SSC; optional second FSC detector

Cuvette Fused silica with 145 x 265 µm channel

Electronics Speed >100,000 events per second with all parameters enabled

Data processing Simultaneous measured peak, area, and width for every channel. 24-bit data for peak and
area. 17-bit data for width with high-resolution linear interpolation at the half height.
Fluidics Sample flow rates 0.0025–2.5 µl/sec

Bulk fluids 4 x 4 L bulk fluid tanks on board for sheath and waste. On board additive concentrate
and cleaner.
Installation Power 95–240 VAC, 50–60 Hz, <500 W

Dimensions (W x D x H) 29 x 27 x 26 in.; 74 x 69 x 66 cm

Weight <240 lb; <110 kg

Temperature 18–25°C; 20–60% RH

and humidity
Air and vacuum supply Included, on-board

Software Operating system Windows 7 Pro

FCS format FCS 3.1

QC Automated quality control with on-board calibration beads

Workstation Intel Core i7-7700 Quad Core; 3.3 GHz

Regulatory CE, Class I (1) laser product

Research use only

For More Information

Web: bio-rad.com/ZE5
Request or download bulletin: 6814

Ordering Information
Catalog # Description Price, U.S. $
ZE5 Cell Analyzer
12004276 ZE5 Cell Analyzer, 3 laser 17 color (405/488/640 nm) 78.00
12004277 ZE5 Cell Analyzer, 3 laser 17 color (488/561/640 nm) 78.00
12004278 ZE5 Cell Analyzer, 4 laser 24 color (405/488/561/640 nm) 78.00
12004279 ZE5 Cell Analyzer, 5 laser 27 color (355/405/488/561/640 nm) 78.00
12014135 ZE5 Cell Analyzer, 5 laser 27 color (355/405/488/561/640 nm) with 7 off UV option A
12014136 ZE5 Cell Analyzer, 5 laser 27 color (355/405/488/561/640 nm) with 7 off UV option B
12014138 ZE5 Cell Analyzer, 4 laser 24 color (405/488/561/640 nm) with SPD
12014141 ZE5 Cell Analyzer, 3 laser 17 color (405/488/640 nm) with SPD
12014139 ZE5 Cell Analyzer, 3 laser 17 color (405/488/561 nm)
12014140 ZE5 Cell Analyzer, 3 laser 20 colors (405/488/561 nm) with SPD
continues

42 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Analysis
bio-rad.com/ZE5 Instruments

Ordering Information
Catalog # Description Price, U.S. $
Accessories
12004403 ZE-Series QC Beads, 5 ml, pack of 3 78.00
1451085 ProLine Rainbow Beads, includes a 5 ml bottle containing a mixture of beads dyed with 8 78.00
different fluorescent intensities for excitation at wavelengths between 365–650 nm
12004271 ZE5 Additive, 4 x 300 ml
12004404 ZE5 Additive Bottle with Tubing, white, includes 450 ml bottle, cap/tubing assembly with filter
and quick connector
12004272 Cytometer Cleaner, 1 L
12004395 ZE5 Cleaner Bottle with Tubing, blue, includes 450 ml bottle, cap/tubing assembly with filter
and quick connector
12004444 ZE5 Cleaner/Additive Bottle, includes 2 x 450 ml bottles
1451083 ProFlow Sort Grade Water, includes 5 x 4 L sterile, endotoxin-free water in gamma-irradiated
fluidic containers
12004397 ZE5 DI Water Container with Tubing, includes 4 L container, cap/tubing assembly with filters
and quick connectors
12004396 ZE5 Waste Container with Tubing, includes 4 L container, cap/tubing assembly with filters and
quick connectors
12004418 ZE5 DI Water Container, 4 L, pkg of 2 78.00
12004402 ZE5 Waste Container, 4 L, pkg of 2 78.00
12004274 5 ml Tubes, 12 x 75 mm with cap, sterile polypropylene, pack of 25
12004445 ZE5 Tube Rack, 5 ml size, 40 spots
12004446 ZE5 Tube Rack, 1.5 ml size, 24 spots

ZE5 Cell Analyzer IQ/OQ Services

Installation qualification (IQ) and operational qualification ■

 aximize instrument uptime — planned instrument
M
(OQ) services for the ZE5 cell analyzer ensure that qualification services ensure your instrument is
your system functions properly and aids in regulatory performing according to specifications
compliance. Both services are completed by a certified ■
Increase your confidence — frequent instrument
service engineer. validation helps minimize loss of precious samples
IQ services include a careful inspection and documentation and valuable time associated with a malfunctioning
of instrument installation. The engineer ensures that the instrument
instrument is properly installed, inspects vital system ■
 emain compliant — official documentation can aid in
R
components, and documents details about the computer meeting regulatory requirements
software and vital system components. For More Information
Web: bio-rad.com/flowiqoq
OQ services verify and document that the instrument is
performing according to the manufacturer’s specifications.
The thorough evaluation includes documentation of
essential system information, evaluation of all key system
components, complete instrument verification, service
system testing, manual and bead-based testing, and signed
documentation of the completion of the IQ/OQ services.

Ordering Information
Catalog # Description Price, U.S. $
12005874 ZE5 Cell Analyzer IQ/OQ Kit, includes IQ and OQ protocols, test reagents, and accessories 78.00

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 43

 Cell Analysis
Instrument Upgrades bio-rad.com/ZE5

Instrument Upgrades

7 Detectors off 355 nm Laser Upgrade Kit

This upgrade kit expands experimental options by Compatible systems: ZE5 systems with 5 lasers
increasing the number of detectors off the 355 nm laser For More Information
from five to seven. Web: bio-rad.com/ZE5

Option A Seven Detectors Fluorophores Option B Seven Detectors Fluorophores

355 Laser 387/11 BUV395 355 Laser 387/11 BUV395
439 DLP (changeable) 439 DLP (changeable)
509/24 BUV496, DAPI 460/22 DAPI
560 DLP 493 DLP
577/15 BUV563 509/24 BUV496
600 DLP (changeable) 530 DLP (changeable)
615/24 BUV615 577/15 BUV563
640 DLP 640 DLP
670/30 BUV661 670/30 BUV661
690 DLP 690 DLP
747/33 BUV737 747/33 BUV737
776 DLP (changeable) 776 DLP (changeable)
780/LP BUV805 780/LP BUV805

Ordering Information
Catalog # Description Price, U.S. $
12009028 7 Detectors off 355 nm Laser Upgrade Kit, Option A 78.00
12009029 7 Detectors off 355 nm Laser Upgrade Kit, Option B 78.00

355 nm Laser Upgrade Kit for ZE5 Cell Analyzer

The UV (355 nm) laser addition is suitable for any ZE5 Compatible systems: any ZE5 system without a UV laser
system without UV laser. It adds the 355 nm laser and bank For More Information
with five detectors. Use this laser upgrade kit to expand Web: bio-rad.com/ZE5
your experimental options in research applications.

Ordering Information
Catalog # Description Price, U.S. $
12009185 355 nm Laser Upgrade Kit for ZE5 Cell Analyzer 78.00

44 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Analysis
bio-rad.com/ZE5 Instrument Upgrades

Everest Software Developer Kit (ZE5 Automation)

The Everest application program interface (API) and ■

It includes a fully functional API client test application,
software development kit (SDK) allow the ZE5 cell analyzer which demonstrates how automation scheduling clients
to interface with existing automation systems. can be implemented using status polling with a minimal
amount of coding effort. Source code is included
■
 he ZE5 API is an industry-standard HTTP-based
T
RESTful web service that is platform- and language- For More Information
Web: bio-rad.com/ZE5
independent
■
 he ZE5 API solution provides all basic ZE5 functionality
T
and many advanced features designed for automated
environments

Ordering Information
Catalog # Description Price, U.S. $
12009761 Everest Software Developer Kit (ZE5 Automation) 78.00

Small Particle Detector (SPD) Upgrade Kit (on 405 nm Laser)

This upgrade kit makes an SPD (secondary FSC detector For More Information
off the 405 nm laser) available for 3 and 4 laser ZE5 Web: bio-rad.com/ZE5
systems with a 405 nm laser. Note: The SPD comes
standard on the 5 laser ZE5 cell analyzer.

Ordering Information
Catalog # Description Price, U.S. $
12009372 Small Particle Detector (SPD) Upgrade Kit (on 405 nm Laser) 78.00

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 45

 Cell Analysis
Instrument Upgrades bio-rad.com/ZE5

Large Capacity Fluidics Options

External fluidics connections reduce day-to-day fluidics Laboratories without pressurized house DI water can
maintenance and enable uninterrupted overnight runs in use the the external DI water carboy and fluidics cart
high-throughput and automation environments. upgrade kit to increase the time the ZE5 cell analyzer can
run unattended. It enables approximately 22 hours of
The following options are available:
uninterrupted run time. Liquids are hot swappable, and
■
External house DI upgrade kit fluidics level warnings from Everest software inform users
that fluidics need attention starting at 2 hours of remaining
■
External DI water carboy and fluidics cart upgrade kit run time.
■
External waste carboy and fluidics cart The external waste carboy and fluidics cart upgrade kit
The external house DI water upgrade kit is suitable for enables approximately 22 hours of unattended waste
laboratories with access to pressurized house DI water. It collection. Liquids are hot swappable.
enables a connection between the ZE5 cell analyzer and For More Information
the laboratory’s source of pressurized house DI water. Web: bio-rad.com/ZE5

Ordering Information
Catalog # Description Price, U.S. $
12009651 External House DI Water Upgrade Kit
12009707 ZE5 External DI Water Carboy and Fluidics Cart Upgrade Kit, includes external DI water 78.00
fluidics cart, power supply, 20 L DI water carboy, and DI water carboy cap vented with integrated
replaceable filter and leak-ree quick disconnect
12009734 ZE5 External Waste Carboy and Fluidics Cart Upgrade Kit, includes external waste fluidics
cart with power supply, waste carboy, and cap for ZE5 waste carboy with overflow sensor, leak-
free quick disconnect, and filtered vent

46 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Analysis
bio-rad.com/ZE5 Reagents

Reagents

Kits and Reagents for Cell Analysis

Bio-Rad offers a variety of easy-to-use kits and reagents suitable for use with the ZE5 cell analyzer.

Ordering Information
Maximum Maximum Optimal
Excitation, Emission, Excitation ZE5 Cell Analyzer
Catalog # Description nm nm Laser, nm Configuration Price, U.S. $
Antibody Labeling Kits
1351011 ReadiLink 405/454 403 454 405 405 laser 460/22 405.00
1351002 ReadiLink 492/516 492 516 488 488 laser 525/35
1351003 ReadiLink 555/570 555 570 561 561 laser 577/15 405.00
1351004 ReadiLink 594/610 594 610 561 561 laser 615/24 405.00
1351005 ReadiLink 633/655 633 655 640 640 laser 670/30
1351006 ReadiLink 647/674 647 674 640 640 laser 670/30
1351007 ReadiLink 680/701 680 701 640 640 laser 720/60
1351008 ReadiLink 700/713 700 713 640 640 laser 720/60
Cell Proliferation
1351202 CytoTrack Blue 403/454 403 454 405 405 laser 460/22 38.00
1351203 CytoTrack Green 511/525 511 525 488 488 laser 525/35 38.00
1351204 CytoTrack Yellow 542/556 542 556 532 561 laser 577/15 38.00
1351205 CytoTrack Red 628/643 628 643 633 or 640 640 laser 670/30
1351201 CFDA-SE Cell Proliferation 492 517 488 488 laser 525/30 38.00
Assay
Cell Viability
1351111 VivaFix 353/442 353 442 355 355 laser 447/60 38.00
1351112 VivaFix 410/450 410 450 405 405 laser 460/22 38.00
1351113 VivaFix 408/512 408 512 405 405 laser 525/50 38.00
1351114 VivaFix 398/550 398 550 405 405 laser 525/50 38.00
1351115 VivaFix 498/521 498 521 488 488 laser 525/35
1351116 VivaFix 547/573 547 573 561 561 laser 577/15
1351117 VivaFix 583/603 583 603 561 561 laser 615/24
1351118 VivaFix 649/660 649 660 640 640 laser 670/30
1351101 Propidium Iodide 538 617 355, 488, or 561 355 laser 670/30
488 laser 593/52
561 laser 615/24
1351102 7-AAD 548 648 488 or 561 488 laser 692/80
561 laser 640/20
Nuclear Dyes
1351303 DAPI 359 461 355 or 405 355 laser 447/60 38.00
or 405 laser 460/22
1351304 Hoechst 33342 350 461 355 355 laser 447/60 or 670/30 38.00

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 47

 Cell Biology Reagents
Antibody Labeling Kits bio-rad.com/readilink1

Cell Biology Reagents

In addition to cell biology reagents on bio-rad.com, Bio-Rad offers a dedicated antibody website —
bio-rad-antibodies.com — specifically designed to meet the unique needs of researchers who use antibodies.
This website showcases the range of our more than 10,000 antibodies and related reagents suitable for flow cytometry,
immunohistochemistry, immunofluorescence, western blotting, immunoprecipitation, and ELISA. The antibody range also
includes the highly validated PrecisionAb antibodies for western blotting and covers key research areas such as immunology,
cancer, veterinary immunology research, and cell biology.

Antibody Labeling Kits

See Also ReadiLink Antibody Labeling Kits

Cell sorting
consumables:
page 37. The following products are available on bio-rad.com.
Cell counting: Antibod
page 27. ReadiLink antibody labeling kits offer easy fluorescence
Dye
conjugation in microscale volumes. Each kit provides
all the essential components to perform two 50 μg Labeled
conjugation reactions with primary antibodies. Available
in a variety of excitation and emission wavelength Quench

combinations, the ReadiLink kits can be used to rapidly

conjugate any antibody of interest for use in multicolor flow Mix and Quench
incubate and
cytometry experiments. incubate
■
Efficient two-step microgram antibody labeling
■
Stable carboxamide bond with fluorescent dye Antibody
■
Quench buffer eliminates background fluorescence Quickly label your antibody of Dye
interest in two easy steps with
For More Information ReadiLink antibody labeling kits. Labeled antibody
Web: bio-rad.com/readilink1
Download bulletin: #10033055 Quenched dye
Request or download bulletin: 6523
Mix and Quench
incubate and
100 100 incubate
Normalized intensity

Normalized intensity

80 80

60 60

40 40

20 20

0 0
250 300 350 400 450 500 550 600 650 700 750 800 850 900 250 300 350 400 450 500 550 600 650 700 750 800 850 900

Wavelength, nm
Wavelength, nm

Excitation wavelength spectrum. ReadiLink dyes 405/454 (–); Emission wavelength spectrum. ReadiLink dyes 405/454 (–);
492/516 (–); 555/570 (–); 594/610 (–); 647/674 (–); 680/701 (–); 492/516 (–); 555/570 (–); 594/610 (–); 647/674 (–); 680/701 (–);
750/780 (–); 790/811 (–). 750/780 (–); 790/811 (–).

48 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Biology Reagents
bio-rad.com/readilink1 Antibody Labeling Kits

Ordering Information
Maximum Maximum Optimal S3e ZE5 ZOE
Fluorophore Excitation, Emission, Excitation Cell Cell Cell
Catalog # Description Equivalent nm nm Laser, nm Sorter Analyzer Imager Price, U.S. $
1351011 ReadiLink 405/454 Pacific Blue 403 454 405 • • 405.00
FITC
1351002 ReadiLink 492/516 Alexa Fluor 488 492 516 488 • • •
DyLight 488
Cy3 405.00
1351003 ReadiLink 555/570 Alexa Fluor 555 555 570 561 • • •
DyLight 550
Texas Red 405.00
1351004 ReadiLink 594/610 Alexa Fluor 594 594 610 561 • • •
DyLight 594
Cy5
1351005 ReadiLink 633/655 Alexa Fluor 633 633 655 640 • •
DyLight 633
Cy5
1351006 ReadiLink 647/674 Alexa Fluor 647 647 674 640 • •
DyLight 650
Cy5.5
IRDye 700
1351007 ReadiLink 680/701 680 701 640 • •
Alexa Fluor 680
DyLight 680
1351008 ReadiLink 700/713 Alexa Fluor 700 700 713 640 • •
Alexa Fluor 750
1351009 ReadiLink 750/780 750 780 640 •
DyLight 755
IRDye 800
1351010 ReadiLink 790/811 Alexa Fluor 790 790 811
DyLight 800

Each ReadiLink kit includes 2 vials of labeling dye, reaction buffer, and dye quench for labeling 2 x 50 µg of antibodies.

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 49

 Cell Biology Reagents
Antibody Labeling Kits bio-rad.com/lynxkits

LYNX Conjugation Kits

5
The following products are available on 10

bio-rad-antibodies.com. 10
4

15 min* or 3 hr Ready to use

LYNX Rapid Conjugation Kit and LYNX Rapid Plus

CD8 A647
3
10

conjugation kits are available in a wide range of options,

including common fluorophores, enzymes, and biotin/ 10
2

streptavidin conjugations. These kits are suitable for use 1

in flow cytometry, enzyme-linked immunosorbent assay Add LYNX modifier to
Add the antibody10modifier
mixture to LYNX mix and
Add LYNX quencher and
purified antibodies incubate >30 min
(ELISA), western blotting, immunohistochemistry, and incubate for 15 min*
10 or 3 hr
0

immunofluorescence applications. Identical protocols for 10

0
10
1
10
2
10
3
10
4
10
5

small-scale and large-scale conjugations allow conjugation CD4 PE

of up to milligram quantities of antibody. Conjugate your 10

10
55 10
10
55

antibodies in three easy steps with only 30 seconds

hands-on time and no purification required. 10
10
44 10
10
44

CD8 DyLight 650

CD8 DyLight650
 ast — 1-step labeling procedure; requires 30 sec
F CD8 A647
CD8 A647
■ 1033 1033
10 10

hands-on time, with an incubation time of either 15 min 22 22

10
10 10
with the LYNX Rapid Plus kits or 3 hr with the LYNX
10

Rapid kits to finish the conjugation 1

10 1
10 10
10
11

■
Simple — can be used in the presence of up to 0
10 0
0
10 0
10 10
0.1% sodium azide and 0.5% bovine serum albumin 0 0
1010 11
1010 2
10102 10 33 4 4
10 1010 101055 00 1
10101 10
1010 22
10 10
33 4 4
10 1010 101055
CD4 PE CD4 PE
■
Instantly ready to use — no additional columns or CD4 PE CD4 PE

dialysis are required after the conjugation process is Comparison of LYNX kits with directly conjugated antibodies.
5
Purified
10
CD4 and CD8 T cells were labeled with LYNX Rapid RPE
finished; the stable, high-quality labeled antibodies are antibody conjugation kit (catalog #LNK021RPE) and LYNX Rapid Plus
ready to use 4
DyLight
10
650 antibody conjugation kit (#LNK241D650), respectively, and
used to stain human peripheral blood. Alternatively, directly labeled
CD8 DyLight650

■
 00% recovery — no postconjugation cleanup required,
1 3
antibodies
10
were used. The staining shown is lymphocytes gated on
which enables 100% yields of labeled antibodies. LYNX the CD3 positive population. CD4 and CD8 positive T cells can be
kits save time and eliminate batch-to-batch variation and 2
identified
10
in both plots. Data acquired on the ZE5 cell analyzer.
loss of antibodies compared to traditional labeling methods PE, phycoerythrin.
1
10
■
Easy scale-up — same procedure is used for labeling * LYNX Rapid Plus kits only.
0
small and large quantities of antibodies; users can initially 10
0 1 2 3 4 5
10 10 10 10 10 10
conjugate micrograms of antibody for trial, then scale up
CD4 PE
to milligram quantities
For More Information
Web: bio-rad.com/lynxkits

50 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Biology Reagents
bio-rad.com/lynxkits Antibody Labeling Kits

Ordering Information
Catalog # Description Price, U.S. $
LYNX Rapid Conjugation Kits
LNK061F LYNX Rapid Fluorescein Antibody Conjugation Kit, 3 conjugations for 200 µg antibody 78.00
LNK062F LYNX Rapid Fluorescein Antibody Conjugation Kit, 1 conjugation for 2 mg antibody 78.00
LNK063F LYNX Rapid Fluorescein Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK021RPE LYNX Rapid RPE Antibody Conjugation Kit, 1 conjugation for 60 µg antibody
LNK022RPE LYNX Rapid RPE Antibody Conjugation Kit, 3 conjugations for 60 µg antibody
LNK023RPE LYNX Rapid RPE Antibody Conjugation Kit, 1 conjugation for 600 µg antibody
LNK024RPE LYNX Rapid RPE Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LNK171PETR LYNX Rapid RPE–Texas Red Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LNK172PETR LYNX Rapid RPE–Texas Red Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LNK173PETR LYNX Rapid RPE–Texas Red Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LNK174PETR LYNX Rapid RPE–Texas Red Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LNK031APC LYNX Rapid APC Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LNK032APC LYNX Rapid APC Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LNK033APC LYNX Rapid APC Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LNK034APC LYNX Rapid APC Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LNK082C LYNX Rapid RPE-Cy5 Antibody Conjugation Kit, 3 conjugations for 60 µg antibody
LNK084C LYNX Rapid RPE-Cy5 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LNK071PERCP LYNX Rapid PerCP Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LNK072PERCP LYNX Rapid PerCP Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LNK073PERCP LYNX Rapid PerCP Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LNK141PERCPCY5.5 LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit, 1 conjugation for 100 µg antibody
LNK142PERCPCY5.5 LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LNK143PERCPCY5.5 LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LNK144PERCPCY5.5 LYNX Rapid PerCP-Cy5.5 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LNK092PECY5.5 LYNX Rapid RPE-Cy5.5 Antibody Conjugation Kit, 3 conjugations for 60 µg antibody
LNK094PECY5.5 LYNX Rapid RPE-Cy5.5 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody 78.00
LNK131APCCY7 LYNX Rapid APC-Cy7 Antibody Conjugation Kit, 1 conjugation for 100 µg antibody 78.00
LNK132APCCY7 LYNX Rapid APC-Cy7 Antibody Conjugation Kit, 3 conjugations for 100 µg antibody
LNK133APCCY7 LYNX Rapid APC-Cy7 Antibody Conjugation Kit, 1 conjugation for 1 mg antibody
LNK134APCCY7 LYNX Rapid APC-Cy7 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LNK111PECY7 LYNX Rapid RPE-Cy7 Antibody Conjugation Kit, 1 conjugation for 60 µg antibody
LNK112PECY7 LYNX Rapid RPE-Cy7 Antibody Conjugation Kit, 3 conjugations for 60 µg antibody
LNK113PECY7 LYNX Rapid RPE-Cy7 Antibody Conjugation Kit, 1 conjugation for 600 µg antibody
LNK114PECY7 LYNX Rapid RPE-Cy7 Antibody Conjugation Kit, 3 conjugations for 10 µg antibody
LYNX Rapid Plus Conjugation Kits
LNK221D488 LYNX Rapid Plus DyLight 488 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK222D488 LYNX Rapid Plus DyLight 488 Antibody Conjugation Kit, 1 conjugation for 2 mg antibody
LNK223D488 LYNX Rapid Plus DyLight 488 Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK201CY3 LYNX Rapid Plus Cy3 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK203CY3 LYNX Rapid Plus Cy3 Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK251RHO LYNX Rapid Plus Rhodamine Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK253RHO LYNX Rapid Plus Rhodamine Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK231D550 LYNX Rapid Plus DyLight 550 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK233D550 LYNX Rapid Plus DyLight 550 Antibody Conjugation Kit, 3 conjugations for 20 µg antibody
LNK211CY5 LYNX Rapid Plus Cy5 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK212CY5 LYNX Rapid Plus Cy5 Antibody Conjugation Kit, 1 conjugation for 2 mg antibody
LNK241D650 LYNX Rapid Plus DyLight 650 Antibody Conjugation Kit, 3 conjugations for 200 µg antibody
LNK243D650 LYNX Rapid Plus DyLight 650 Antibody Conjugation Kit, 3 conjugations for 20 µg antibody

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 51

 Cell Biology Reagents
Cell Proliferation Assays bio-rad.com/cellproliferationassay

Cell Proliferation Assays

An increase or decrease in cell growth and division can be a key result in cytotoxicity and apoptosis assays and can be used
to determine the effect of experimental treatments on cellular replication. We offer a broad range of antibodies, kits, and dyes
to monitor proliferation. Proliferation can be measured through dye incorporation into live cells, by quantifying key proliferation
markers, or by assessing incorporation of the thymidine analog BrdU into DNA.

Cell Proliferation Assay Reagents

■
 imple — water soluble, just add and measure the
S
formula in cell cultures
■
 lexible — colorimetric and fluorometric detection for
F
The following products are available on both suspension and adherent cell lines
bio-rad-antibodies.com. ■
 ontoxic — alamarBlue does not kill cells and is
N
safe to the user and the environment
alamarBlue is a cell proliferation assay that provides a rapid,
sensitive, and economical way to quantitatively measure ■
Reliable — highly referenced for cytotoxicity and
cell proliferation and cytotoxicity in various human and viability assays
animal cell lines. It can also be used to study mycobacteria, ■
High sensitivity — detects as few as 50 cells
bacteria, and fungi. It is a preferred alternative reagent to ■
 table — proprietary buffering agents ensure alamarBlue
S
similar cell proliferation assays, including MTT, XTT, 3H
is suitable for time-course studies
thymidine, and neutral red.
■
 conomical — lack of cell lysis allows cells to be
E
For More Information cultured for subsequent assays
Web: bio-rad.com/cellproliferationassay

Ordering Information
Catalog # Description Price, U.S. $
BUF012A alamarBlue Reagent, 25 ml 305.00
BUF012B alamarBlue Reagent, 100 ml 305.00

52 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Biology Reagents
bio-rad.com/cytotrack Cell Proliferation Assays

CytoTrack Cell Proliferation Assay

The following products are available on bio-rad.com. CytoTrack Blue 403/454 (DPBS) CytoTrack Green 511/525 (DPBS)
100 Days 5 and 6 100 Days 5 and 6
CytoTrack cell proliferation assays efficiently stain live not collected not collected

cells for excellent resolution of up to 10 generations in a 80 80

Normalized to mode

Normalized to mode
population of dividing cells. Using a proprietary chemistry,
60 60
the CytoTrack dye reacts with intracellular proteins and is
effectively retained in the cell without efflux. As cell division 40 40
occurs, the dye is successively halved and the different
generations can be determined based on the decrease in 20 20

fluorescence intensity.
0 0
■
Dyes are directly added to cell culture media or buffer 0 103 104 105
405 nm laser, 460/22 filter
0 103 104 105
488 nm laser, 525/35 filter
■
 ompatible with intracellular analysis using standard
C CytoTrack Yellow 542/556 (media) CytoTrack Red 628/643 (DPBS)
formaldehyde-containing fixatives and saponin-based 100 Days 5 and 6 100 Days 5 and 6
not collected
permeabilization buffers not collected

80 80
 asily combine CytoTrack dyes with fluorescent proteins
E

Normalized to mode
Normalized to mode
■

or antibody-labeled fluorophores for multicolor cell analysis 60 60

40 40
Cell-retaining
group
Esterase 20 20
cleavage

Incubate
Fluorescence with cells 0 0
blocker
0 103 104 105 0 103 104 105
561 nm laser, 577/15 filter 640 nm laser, 670/30 filter
Masked
fluorophore CFDA-SE (DPBS)
100 Days 5 and 6 Gap between
Day 0: Far right peak
not collected days 0 and 1 Days 1–10: Peaks in between
The nonfluorescent dye is cleared upon cell entry with the cell- Negative: Far left peak
retaining group and then covalently binds to intracellular protein, 80
Normalized to mode

allowing it to remain in the cell during division.

60
For More Information
Web: bio-rad.com/cytotrack 40
Download bulletin: #10033110
Request or download bulletin: 6524 20

0
0 103 104 105
488 nm laser, 525/35 filter

CytoTrack and CFDA-SE performance on the ZE5 cell analyzer.

1x CytoTrack solution in either cell culture media or buffer was added
to 1 x 106 Jurkat cells/ml. Cells were incubated in the dark at room
temperature for 15 min, then washed and resuspended with cell culture
media or buffer. Half were run on the ZE5 cell analyzer (day 0) and the
other half left to grow. Each day, half the sample was removed to run
on the ZE5 cell analyzer and the cell culture was replenished. DPBS,
Dulbecco’s phosphate buffered saline.

Ordering Information
Maximum Maximum Optimal S3e ZE5 ZOE Price, U.S. $
Excitation, Emission, Excitation Laser, Cell Cell Cell
Catalog # Description nm nm nm Sorter Analyzer Imager
1351202 CytoTrack Blue 403/454 403 454 405 • • 305.00
1351203 CytoTrack Green 511/525 511 525 488 • • 305.00
1351204 CytoTrack Yellow 542/556 542 556 532 • • 305.00
1351205 CytoTrack Red 628/643 628 643 633 or 640 • • 305.00

Each kit includes 4 vials of dye and 1 vial DMSO for 200 cell proliferation tests.

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 53

 Cell Biology Reagents
Cell Proliferation Assays bio-rad.com/cellproliferationassay

CFDA-SE Cell Proliferation Assay

100
The following products are available on bio-rad.com.
80
CFDA-SE cell proliferation assay provides carboxyfluorescein
diacetate succinimidyl ester (CFDA-SE) in a convenient and

Intensity, AU
60
CFDA-SE excitation/
easy-to-use format. CFDA-SE is a cell-permeable dye that emission is 492/517 nm
40
fluoresces after the acetate groups are cleaved by intracellular in 0.1 M NaOH. Excitation
esterases, creating carboxyfluorescein succinimidyl ester 20 wavelength (—); emission
(CFSE). As the succinimidyl ester group reacts with primary wavelength (—). AU,
absorbance unit.
amines of intracellular proteins, CFSE is retained in the cell 450 500 550 600 650
and is successively halved during cell divisions. Wavelength, nm

■
Visualize up to 8 cell divisions Staining buffer Culture media
100 100
■
 inimize contamination and reduce dilution steps
M
with small vial size 80 80

Normalized to mode

Normalized to mode
60 60
For More Information
Web: bio-rad.com/cytotrack 40 40

20 20

0 0
102 103 104 105 10 6 102 103 104 105 10 6
CytoTrack Green 511/525 CFDA-SE

CytoTrack resolves up to ten cell divisions compared to eight for

CFDA-SE.

Ordering Information
Maximum Maximum Optimal S3e ZE5 ZOE Price, U.S. $
Excitation, Emission, Excitation Laser, Cell Cell Cell
Catalog # Description nm nm nm Sorter Analyzer Imager
1351201 CFDA-SE Cell Proliferation 492 517 488 • • 305.00
Assay

Includes 5 x 100 µg vials 5(6)-carboxyfluorescein diacetate succinimidyl ester, CFDA-SE.

54 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Biology Reagents
bio-rad.com/cellproliferationassay Cell Proliferation Assays

Cell Proliferation Detection Antibodies

The following products are available on

bio-rad-antibodies.com. 105

The thymidine analog 5'-bromo-2'-deoxyuridine (BrdU) is a

104
popular reagent in various scientific disciplines ranging from S phase

BrdU FITC
cancer to neuroscience research. The chemical is
frequently added to samples, for example mammalian cell 10 3

lines, and subsequently incorporated into newly synthesized

DNA during replication. 102
G1 G2
0

To measure cell proliferation, the incorporated BrdU is  –102

detected with anti-BrdU antibodies, for example the mouse

0
0

00

00

00

00
,0

0,

0,

0,

0,
50
anti-BrdU antibody, clone Bu20a. However, to ensure that

15

25
10

20
Propidium iodide
the antibody is able to bind to BrdU, DNA treatment prior
to antibody staining is required (Liboska et al. 2012). In the Staining profile of anti-BrdU antibody. BrdU-labeled human
literature, endonucleases such as deoxyribonuclease I lymphoma cells were stained with mouse anti-BrdU antibody, clone
(DNase I) and acids such as hydrochloric acid are frequently BU20a, at a 1/100 dilution overnight at 4°C. A secondary antibody,
FITC-conjugated goat anti-mouse IgG (H/L) (#STAR117F) was used
used to cleave or denature the DNA to make incorporated at a 1/50 dilution. Total DNA staining was performed with ReadiDrop
BrdU accessible (Liboska et al. 2012). propidium iodide (#1351101). FITC, fluorescein isothiocyanate.

Our mouse anti-BrdU antibody, clone Bu20a (catalog

Detection of BrdU is commonly performed in combination
#MCA2483), has been tested in flow cytometry and
with total DNA staining. When performing cell cycle analysis,
immunocytochemistry. The product has been referenced in
propidium iodide tends to be the DNA stain of choice.
12 publications. It is available in three different sizes (0.2 mg,
0.1 mg, and 20 µg) and is conjugated to FITC. In addition to Ki67, proliferating cell nuclear antigen (PCNA), and
flow cytometry and immunocytochemistry, the Bu20a clone minichromosome maintenance protein 2 (MCM2) are key
is recommended for immunohistochemistry. proteins for regulating cellular growth and proliferation.

Ordering Information
Species Price, U.S. $
Catalog # Description Clone Reactivity Applications Host Formats
MCA2483 BrdU Bu20a − FC,* IF/ICC, Mouse Pur., FITC 305.00
IHC-F, IHC-P*
AHP2405 BrdU − − F,* IF/ICC, IF,* P Rabbit Pur.
HCA117 Ki67 2815 DISCONTINUED
Human E, IHC-P* HuCAL Pur. 305.00
HCA053 Ki67 2531 Bovine, dog, E, IHC-F, HuCAL® Pur. 305.00
human IHC-P,* WB
HCA006 Ki67 AbD2531 Bovine, dog, E, IF/ICC, IHC-F, HuCAL Pur. 305.00
(Fab) human IHC-P,* WB
VMA00259 MCM2 PrecisionAb OT13B6 Human, mouse, WB Mouse Pur. 305.00
Antibody rat
MCA1859 MCM2 CRCT2.1 Human IHC-P,* WB Mouse Pur. 305.00
VMA00018 PCNA PrecisionAb − Human, mouse, WB Mouse Pur. 305.00
rat
MCA1558 PCNA PC10 Broad FC,* IHC-F, Mouse Pur., FITC, 305.00
IHC-P, IP, WB HRP
AHP1419 PCNA Polyclonal − Bovine, dog, E, IHC-P, WB Rabbit Pur. 305.00
human, mouse, rat
Applications: E, ELISA; FC, flow cytometry; IF/ICC, immunofluorescence/immunocytochemistry; IHC-F, immunohistology-frozen;
IHC-P, immunohistology-paraffin; IP, immunoprecipitation; WB, western blotting.
Formats: FITC, fluorescein isothiocyanate; HRP, horseradish peroxidase; Pur., purified.
* Requires special treatment. Refer to product datasheet.

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 55

Discontinued products are no longer available.
 Cell Biology Reagents
Cell Viability Assays bio-rad.com/vivafix

Cell Viability Assays

VivaFix Cell Viability Assay

The following products are available on bio-rad.com. A B

VivaFix cell viability assays provide a sensitive method for

determining the viability of live or fixed mammalian cells
by flow cytometry, cell sorting, and microscopy.
Using proprietary dyes that covalently bind to primary
amines, VivaFix cell viability assays provide at least a
100-fold difference in fluorescence intensity between live
and dead cells. The brightness of VivaFix dyes is
preserved upon treatment with fixative agents. VivaFix cell viability assay chemistry. A, VivaFix dyes bind to the cell
surface primary amines of viable cells; B, in dead cells, where the plasma
■
100-fold discrimination between dead and live cells membrane is compromised, VivaFix dyes are able to permeate the cell
and also react with intracellular primary amines. As a result, a greater
■
Compatible with fixed and unfixed cells
number of fluorophores is associated with dead cells and at least a
■
8 different excitation/emission wavelength combinations 100-fold difference in fluorescence intensity is measured between the live
and the dead cells, thereby allowing an easier discrimination between the
two populations.

A A
256
1,000,000

Dead
800,000 cells Dead
192
cells

Live
600,000
SSC area

SSC area

cells Live
128 cells

400,000

64
200,000

0 0
10 0 101 102 103 104 10 0 101 102 103 104 105
VivaFix 498/521 VivaFix 353/442
B B
256
1,000,000

Dead
800,000 cells 192 Dead
cells

Live
SSC area

SSC area

600,000 cells Live

128 cells

400,000

64
200,000

0 0
10 0 101 102 103 104 10 0 101 102 103 104 105
VivaFix 498/521 VivaFix 353/442

Assessment of cell viability using VivaFix and the S3e cell sorter. Assessment of cell viability using VivaFix and the ZE5 cell analyzer.
Jurkat cells were stained with the VivaFix 498/521 dye, fixed with Jurkat cells were heat shocked at 65°C for 5 min, then washed and
3.7% formaldehyde (A) or not fixed (B), and analyzed with the S3e cell suspended at 2 x 106 cells/ml in phosphate buffered saline. Cells were
sorter. SSC, side scatter. incubated with VivaFix 353/442 at 37°C for 30 min in the dark. A, half
of the cells fixed in 1% paraformaldehyde; B, half of the cells not fixed.
Identical gates for all samples were used during analysis on the ZE5
For More Information cell analyzer. SSC, side scatter.
Web: bio-rad.com/vivafix

56 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Biology Reagents
bio-rad.com/readidrop1 Cell Viability Assays

Ordering Information
Maximum Maximum Optimal S3e ZE5 ZOE Price, U.S. $
Excitation, Emission, Excitation Laser, Cell Cell Cell
Catalog # Description nm nm nm Sorter Analyzer Imager
1351111 VivaFix 353/442 353 442 355 • • 305.00
1351112 VivaFix 410/450 410 450 405 • •
1351113 VivaFix 408/512 408 512 405 • •
1351114 VivaFix 398/550 398 550 405 • •
1351115 VivaFix 498/521 498 521 488 • • •
1351116 VivaFix 547/573 547 573 561 • • • 305.00
1351117 VivaFix 583/603 583 603 561 • • • 305.00
1351118 VivaFix 649/660 649 660 640 • • 305.00

Each VivaFix assay includes 4 x 50 test vials and 250 µl of DMSO.

ReadiDrop Cell Viability Assays

The following products are available on bio-rad.com.

Designed for ease of use in cell sorting and flow cytometry applications, ReadiDrop cell viability assays remove the
traditional manual weighing, pipetting, and dilution steps required with commonly available cell viability dyes. Simply add
one or two drops to assess the health of your cells.

ReadiDrop Propidium Iodide ReadiDrop 7-AAD

Cell impermeant nucleic acid–binding dye that binds to
■
Cell impermeant DNA binding dye with a high affinity
■

double-stranded DNA/RNA for GC-rich regions of double-stranded DNA

■
Ready-to-use formulation in PBS without preservatives ■
Ideal for cell sorting and flow cytometry
■
Stable at room temperature ■
Stable at room temperature
120 120
Excitation/emission = 538/617 nm Excitation/emission = 548/648 nm
+ DNA in pH 7.0 buffer + DNA in pH 7.0 buffer
100 100
Intensity, absorbance unit (AU)

Intensity, absorbance unit (AU)

80 80

60 60

40 40

20 20

0 0
400 500 600 700 400 500 600 700 800
Wavelength, nm Wavelength, nm

Excitation and emission curves for propidium iodide and 7-AAD.

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 57

 Cell Biology Reagents
Cell Viability Assays bio-rad.com/readidrop1

ReadiDrop propidium iodide ReadiDrop 7-AAD

Live Dead Live Dead

cells cells cells cells
105 84
Count

Count
70 56

35 28

0 0
10 0 101 102 103 104 10 0 101 102 103 104
488 nm laser, 593/52 filter 488 nm laser, 692/80 filter

Live Dead Live Dead

cells cells cells cells

123 66

Count
Count

82 44

41 22

0 0
10 0 101 102 103 104 10 0 101 102 103 104
561 nm laser, 615/24 filter 561 nm laser, 640/20 filter

Assessment of cell viability using ReadiDrop reagents and the ZE5 cell analyzer. Jurkat cells were heat shocked at 65°C for 5 min. Cells were
washed and resuspended at 2 x 106 cells/ml in phosphate buffered saline (PBS). ReadiDrop propidium iodide or 7-AAD (20 μl) was added to
0.5 ml cells and incubated for 15 min at room temperature in the dark. Cells were washed once in PBS prior to acquisition on the ZE5 cell analyzer.

For More Information

Web: bio-rad.com/readidrop1
Download bulletin: #10049240
Request or download bulletin: 6522

Ordering Information
Maximum Maximum Optimal S3e ZE5 ZOE Price, U.S. $
Excitation, Emission, Excitation Laser, Cell Cell Cell
Catalog # Description nm nm nm Sorter Analyzer Imager
1351101 Propidium Iodide 538 617 561 or 488 • • 305.00
1351102 7-AAD 548 648 561 or 488 • • 305.00

Each assay includes 3 x 3 ml bottles of ready-to-use reagent suspended in PBS for dead cell exclusion in cell sorting
and flow cytometry applications.

58 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Biology Reagents
bio-rad.com/calibration Flow Cytometry Calibration and Validation Reagents

Flow Cytometry Calibration and Validation Reagents

Standards and Stabilizers

RPE Reference Standard

RPE reference standard consists of one vial of
microspheres that are uniform in both size and
The following products are available on fluorescence and are approximately the size of human
bio-rad-antibodies.com. lymphocytes (7–9 µm). The excitation and emission spectra
of the microspheres match those of cell samples labeled
Flow Cytometry Absolute Count Standard with RPE. It is used to provide qualitative data when
Flow Cytometry Absolute Count Standard is a suspension compared to a sample but can also be used to calibrate
of microspheres that are approximately the size of human the flow cytometer.
lymphocytes (7–9 µm) and are presented at a known
concentration. They are internally labeled with multiple Protein Stabilizer
fluorochromes and the combination of dyes allows the Protein stabilizer stabilizes antibodies and other proteins
beads to be excited by a common argon laser (488 nm) or biomolecules in solution. It may be used to stabilize
and emit in the three standard channels of a flow cytometer ready-to-use prediluted standards and controls used in
(FL1, FL2, and FL3). immunoassays.
For More Information
Web: bio-rad.com/calibration

Ordering Information
Catalog # Description Price, U.S. $
FCSC580 Flow Cytometry Absolute Count Standard, 1 bottle containing 10 ml of microspheres, 20 tests 78.00
FCSC899B RPE Reference Standard, 1 x 5 ml bottle of labeled microbeads for 100 tests
BUF069A Protein Stabilizer, 100 ml of ready-to-use liquid

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 59

 Cell Biology Reagents
Flow Cytometry Calibration and Validation Reagents bio-rad.com/calibration

Calibration Reagents

Quantum FITC-5 MESF is composed of five calibrated

fluorescent populations in one tube, each with different levels
of FITC fluorescence intensity, and one blank population
The following products are available on of uniform microspheres that are approximately the size
bio-rad-antibodies.com. of human lymphocytes (7–9 µm). Quantum R-PE MESF is
composed of four calibrated fluorescent populations, with
Quantum kits are used for the quantitation of fluorescence different levels of RPE fluorescence intensity.
intensity in molecules of equivalent soluble fluorochrome
(MESF) units. When used in conjunction with Simply Quantum Simply Cellular mouse IgG is composed of four
Cellular microspheres, this kit also allows quantitation of coated populations and one blank population of uniform
antibody binding capacity (ABC). The fluorescence intensity microspheres that are approximately the size of human
of each of the populations in the kit has been calibrated lymphocytes (7–9 µm). Each of the four sets is coated with
against solutions of laser-grade fluorescent dye in units of goat anti-mouse IgG (Fc-specific) with different calibrated
MESF dye per microsphere. The Certified Blank population antibody binding capacities (ABC) for mouse monoclonal
is used to measure the fluorescence detection threshold of antibodies. The blank population has no specific binding
the instrument. capacity for mouse IgG. When the bead populations are
labeled in the same manner as the cells to be analyzed,
■
 uantitation of fluorescence intensity of samples
Q they provide a means for constructing a QuickCal
in terms of MESF calibration curve (ABC values vs. fluorescence intensity),
■
 etermination of instrument fluorescence
D from which samples may be “read.” Cellular antigen
detection threshold expression may thus be quantitated in ABC units.
■
Determination of instrument linearity For More Information
Web: bio-rad.com/calibration
■
 ata comparison over time and between
D
multiple instruments

Ordering Information
Catalog # Description Price, U.S. $
FCSC555A Quantum FITC-5 MESF, 1 x 1 ml bottle of unlabeled microbeads, 5 x 1 ml bottles of labeled 78.00
microbeads for 20 tests
FCSC555B Quantum FITC-5 MESF, 1 x 5 ml bottle of unlabeled microbeads, 5 x 5 ml bottles of labeled
microbeads for 100 tests
FCSC555PA Quantum FITC-5 MESF (Pre-Mixed), 1 x 1 ml bottle of unlabeled microbeads, 5 x 1 ml bottles
of labeled microbeads for 20 tests
FCSC555PB Quantum FITC-5 MESF (Pre-Mixed), 1 x 5 ml bottle of unlabeled microbeads, 5 x 5 ml bottles
of labeled microbeads for 100 tests
FCSC827A Quantum R-PE MESF Medium Level, 1 x 1 ml bottle of unlabeled microbeads, 4 x 1 ml bottles
of labeled microbeads for 20 tests
FCSC827B Quantum R-PE MESF Medium Level, 1 x 5 ml bottle of unlabeled microbeads, 4 x 5 ml bottles
of labeled microbeads for 100 tests
FCSC815A Quantum Simply Cellular Mouse IgG, 1 x 1 ml bottle of unlabeled microbeads, 4 x 1 ml bottles
of labeled microbeads for 20 tests
FCSC815B Quantum Simply Cellular Mouse IgG, 1 x 5 ml bottle of unlabeled microbeads, 4 x 5 ml bottles
of labeled microbeads for 100 tests
FCSC815C Quantum Simply Cellular Mouse IgG, 2 x 7 ml bottle of unlabeled microbeads, 8 x 7 ml bottles 78.00
of labeled microbeads for 280 tests

60 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Biology Reagents
bio-rad.com/flowcytometryreagents Flow Cytometry General Reagents

Flow Cytometry General Reagents

Accessory Reagents

Leucoperm Reagent
Leucoperm reagents allow intracellular antigen analysis
with the same ease as surface antigens. The only
The following products are available on prerequisite is the availability of suitable antibody
bio-rad-antibodies.com. conjugates. Most commercially available monoclonal
antibody conjugates can be used with Leucoperm
Human Seroblock reagents. Some determinants are sensitive, however, to
Human Fc receptors are expressed on a variety of immune the fixation step involved. This and the optimal fixation
cell types including monocytes, macrophages, B cells, time may have to be determined experimentally for each
granulocytes, and dendritic cells. Cells that express Fc antibody conjugate.
receptors can produce false positive immunofluorescent
staining due to the Fc receptors binding Ig. Human Staining Buffer
seroblock is designed to prevent such nonspecific staining Bio-Rad’s staining buffer contains bovine calf serum. It has
without interfering with appropriate target staining. Human been optimized for staining both live and fixed cells with
seroblock is compatible with anti-human antibodies antibodies. It is suitable for use with both conjugated and
targeting Fc receptors in flow cytometry. unconjugated antibodies and can be used as an antibody
dilution media and a wash buffer. The addition of bovine
Mouse Seroblock FcR calf serum reduces background staining of target cells.
Mouse seroblock FcR is a rat antibody that recognizes The staining buffer also contains sodium azide to minimize
mouse CD16 and CD32 cell surface proteins also known receptor capping.
as FcRgIII and FcRgII, respectively. The function of these
proteins is to bind IgG molecules via their Fc regions as part Fixation Buffer
of the adaptive immune response. CD16 and/or CD32 are The fixation buffer is a paraformaldehyde-based fixative that
expressed by a wide variety of cells, including monocytes, is suitable for use with most cell types. Paraformaldehyde-
macrophages, B lymphocytes, granulocytes, NK cells, containing fixation buffer is essential for intracellular staining
dendritic cells, and some activated T lymphocytes. The prior to permeabilization.
expression of CD16/CD32 antigens can lead to nonspecific Intracellular Permeabilization/Wash Buffer
binding of test monoclonal antibodies in staining This permeabilization and wash buffer is for intracellular
procedures, resulting in high background staining on a wide staining. Access to intracellular targets, such as cytokines
range of cells. This nonspecific binding may be blocked by and cytoplasmic proteins, requires permeabilization of the
pre-incubation of target cells with mouse seroblock FcR, cell membrane after fixation. This buffer has minimal effect
resulting in cleaner staining. on cell morphology. The buffer is supplied as a 10x
Neutralite Avidin:Cy5 or RPE solution for dilution in water. It can also be used as a
Neutralite avidin is a deglycosylated version of egg white wash buffer during intracellular staining to help minimize
avidin that retains its affinity for biotin but has a near-neutral background staining.
pH that minimizes nonspecific interactions.
Streptavidin:APC or FITC
Streptavidin is designed as a secondary reagent for use
in flow cytometry in conjunction with biotinylated primary
antibodies. Its conjugates show negligible nonspecific
binding to nonbiotinylated macromolecules, and therefore
produce very low backgrounds.

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 61

 Cell Biology Reagents
Flow Cytometry General Reagents bio-rad.com/flowcytometryreagents

Protein Transport Inhibitors Activation Reagents

Protein transport inhibitors can be used to enhance Bio-Rad offers two cell activation reagents, with and
intracellular cytokine staining. As cytokines and chemokines without the protein transport inhibitor Brefeldin A. They
are usually secreted, use of these inhibitors traps the contain optimized concentrations of phorbol 12-myristate-
proteins within the cell, leading to a buildup of cytokines in 13-acetate (PMA) and ionomycin, which can be used to
the Golgi apparatus and endoplasmic reticulum. The choice activate your cells of interest. Available as a 500x solution
of and culture conditions for these inhibitors may need to in DMSO, they are suitable for activation of cells and
be optimized depending on the species and cytokines stimulating cytokine secretion.
being stained. Monensin acts by interfering with the Na+/H+ For More Information
transport in Golgi apparatus transmembranes. Brefeldin A Web: bio-rad.com/flowcytometryreagents
acts by redistributing proteins from the Golgi apparatus to
the endoplasmic reticulum.
Red Cell Lysing Buffer
The red cell lysing buffer, erythrolyse, is available as a 10x
solution that is stable for 1 year at room temperature. It is
suitable for the lysing of red blood cells in human, rat, and
mouse blood.

Ordering Information
Catalog # Description Price, U.S. $
BUF070A Human Seroblock, includes a solution of buffer and blocking agents for 50 tests 78.00
BUF070B Human Seroblock, includes a solution of buffer and blocking agents for 200 tests
BUF041A Mouse Seroblock FcR, includes 0.1 mg of purified IgG
BUF041B Mouse Seroblock FcR, includes 0.5 mg of purified IgG
STAR119 Streptavidin:APC, includes lyophilized product for 100 tests
STAR2B Streptavidin:FITC, includes 1 mg of streptavidin:FITC at 1 mg/ml
BUF09 Leucoperm Reagent, fixation and permeabilization mediums for 50 tests
BUF09B Leucoperm Reagent, fixation and permeabilization mediums for 250 tests
BUF09C Leucoperm Reagent, fixation and permeabilization mediums for 1,000 tests 78.00
BUF073 Staining Buffer, 500 ml
BUF071 Fixation Buffer, 100 ml
BUF072 Intracellular Permeabilization/Wash Buffer, 10x solution, 100 ml
BUF074 Monensin, 1000x solution, 1 ml
BUF075 Brefeldin A, 1000x solution, 1 ml
BUF04B Red Cell Lysing Buffer, 10x solution, 100 tests/20 ml
BUF04C Red Cell Lysing Buffer, 10x solution, 500 tests/100 ml
BUF036A Phosphate Buffered Saline (PBS), 10x, 1 L
BUF077A Cell Stimulation Reagent (with Brefeldin A), 500x solution, 0.1 ml
BUF076A Cell Stimulation Reagent (without Brefeldin A), 500x solution, 0.1 ml

62 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Cell Biology Reagents
bio-rad.com/PureBluHoechst33342 Nuclear Staining Dyes

Nuclear Staining Dyes

PureBlu Hoechst 33342 Nuclear Staining Dye See Also

ReadiLink antibody
labeling kits:
The following products are available on bio-rad.com. 100 page 48.
VivaFix cell
PureBlu Hoechst 33342 nuclear staining dye is a highly 80 viability assay:
page 56.
pure formulation of Hoechst 33342 fluorescent dye.

Normalized intensity
Cell proliferation
It is available in a user-friendly format that allows easy 60
assays: page 52.
preparation of the working solution without any weighing
40
step and with a single dilution after resuspension.
PureBlu Hoechst 33342 dye permeates cell membranes 20
and binds double-stranded DNA, which enables
identification of nuclei within cells. It has a maximum 0
300 400 500 600 700
excitation wavelength of 350 nm in the ultraviolet range
Wavelength, nm
and can be optimally detected in the blue channel with
a maximum emission wavelength of 461 nm. Excitation and emission spectra of PureBlu Hoechst 33342 dye.

Product features include: For More Information

Web: bio-rad.com/PureBluHoechst33342
■
Easy to reconstitute without any weighing step Download bulletin: #10043283
■
Optimal for live cell staining Request or download bulletin: 6607
■
Compatible with multicolor experiments

Ordering Information
Maximum Maximum Optimal S3e ZE5 ZOE Price, U.S. $
Excitation, Emission, Excitation Laser, Cell Cell Cell
Catalog # Description nm nm nm Sorter Analyzer Imager
1351304 PureBlu Hoechst 33342 350 461 355 • • 305.00

Includes 5 x 56 µg vials of Hoechst 33342 powder.

PureBlu DAPI Nuclear Staining Dye

The following products are available on bio-rad.com.

PureBlu DAPI nuclear staining dye is a highly pure formulation of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)
fluorescent dye. It is available in a user-friendly format that allows easy preparation of the working solution without any
weighing step and with a single dilution after resuspension. PureBlu DAPI dye permeates cell membranes and binds double-
stranded DNA, which enables identification of nuclei within cells. It has a maximum excitation wavelength of 359 nm in the
ultraviolet range and can be optimally detected in the blue channel with a maximum emission wavelength of 461 nm.
Product features include:
■
Easy to reconstitute without any weighing step
■
Optimal for fixed cell staining
■
Compatible with multicolor experiments
For More Information
Web: bio-rad.com/PureBluDAPI
Download bulletin: #10043282
Request or download bulletin: 6607

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 63

 Cell Biology Reagents
Nuclear Staining Dyes bio-rad.com/PureBluDAPI

120 A
100
Normalized intensity

80

60

40

20

0
300 350 400 450 500 550 600
Wavelength, nm

Excitation and emission spectra of PureBlu DAPI dye.

256

Dead
cells B
192
SSC area

Live
128 cells

64

0
10 0 101 102 103 104 105
355 nm laser, 447/60 filter

Assessment of cell viability using PureBlu DAPI and the ZE5 cell
analyzer. Jurkat cells were heat shocked at 65°C for 5 min. Cells
were washed and resuspended at 2 x 106 cells/ml in phosphate
buffered saline. 100x PureBlu DAPI (5 μl) was added to 0.5 ml cells
and incubated for 15 min at room temperature in the dark. Cells were
then run on the ZE5 cell analyzer. SSC, side scatter.
PureBlu dyes are the optimal choice for staining the DNA of
eukaryotic cells. HeLa cells were transfected with a green fluorescent
protein (GFP)–expressing plasmid DNA, stained, and viewed in a six-
well dish using a ZOE fluorescent cell imager. A, cells counterstained
with PureBlu DAPI nuclear staining dye; B, merged view of cells
stained with PureBlu DAPI and GFP.

Ordering Information
Maximum Maximum Optimal S3e ZE5 ZOE Price, U.S. $
Excitation, Emission, Excitation Laser, Cell Cell Cell
Catalog # Description nm nm nm Sorter Analyzer Imager
1351303 PureBlu DAPI 359 461 355 • • 305.00

Includes 5 x 50 µg vials of DAPI powder.

64 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Regulated Cell Death Reagents
bio-rad.com/apoptosis Apoptosis Reagents

Regulated Cell Death Reagents

Cell death is a crucial step in organism development, tissue homeostasis, and host defense. There are many mechanisms of
regulated cell death (RCD) such as apoptosis, pyroptosis, necroptosis, and autophagy, where signal transduction molecules
are not only initiated but also involved in the propagation of cell death.
Initiation of RCD can result from tissue-turnover, known as programmed cell death (PCD), or disruption of the intercellular
or extracellular environment (stress driven RCD). Regulated cell death is involved in immunological processes including
elimination of infected cells, removal of effector immune cells, and lymphocyte development.
For More Information
Web: bio-rad.com/rcd

Apoptosis Reagents
Apoptosis, one of the main forms of programmed cell death, is essential for removing damaged or old cells and is involved
in organism development, tissue homeostasis, and host defense of infected or transformed cells. Reagents to common
apoptosis markers allow determination of cellular apoptosis levels. Activation of the caspase cascade initiates this process
through either the intrinsic or extrinsic pathway.

Annexin V Kits

PS is an indication of early apoptosis (Fadeel and Xue,

2009). Annexin V has a high binding affinity for PS in the
presence of Ca2+ and is therefore a commonly used tool
for detecting apoptotic cells.
The following products are available on
bio-rad-antibodies.com.
■
 pplicable for multicolor flow cytometry experiments;
A
available as conjugates, including biotin, FITC, PE,
Early apoptotic cells can be detected with the help of and APC
annexin V, a protein encoded by the ANXA5 gene, which
acts as an anticoagulant. Annexin V, like other members
■
Includes the viability dye propidium iodide for distinction
of the annexin protein family, binds to specific membrane between apoptotic/secondary necrotic and apoptotic cells
proteins. During the early stages of apoptosis, the ■
 abeled annexin V, included in the kits, has been
L
plasma membrane is rearranged, resulting in changes conjugated such that the native phospholipid binding
to the cell membrane’s phospholipid asymmetry. One properties of annexin V remain unaltered
of the effects of these rearrangements is the display of
phosphatidylserine (PS) at the outer plasma membrane. For More Information
Web: bio-rad.com/apoptosis
In healthy, quiescent cells, PS is exclusively located
on the inner membrane and therefore the exposure of

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 65

 Regulated Cell Death Reagents
Apoptosis Reagents bio-rad.com/apoptosis

Ordering Information
Catalog # Description Price, U.S. $
ANNEX20B Annexin V:Biotin Assay Kit, 1 x 0.1 ml annexin V:Biotin, 1 x 1.6 ml propidium iodide, 1 x 50 ml vial binding buffer for 20 tests00
ANNEX100B Annexin V:Biotin Assay Kit, 1 x 0.5 ml annexin V:Biotin, 1 x 1.6 ml propidium iodide, 1 x 50 ml vial binding buffer for 100 tests
ANNEX300B DISCONTINUED
Annexin V:Biotin Assay Kit, 1 x 1.5 ml annexin V:Biotin, 2 x 1.6 ml propidium iodide, 1 x 50 ml vial binding buffer for 300 tests
ANNEX20F Annexin V:FITC Assay Kit, 1 x 0.1 ml annexin V:FITC, 1 x 1.6 ml propidium iodide, 1 x 50 ml vial binding buffer for 20 tests
ANNEX100F Annexin V:FITC Assay Kit, 1 x 0.5 ml annexin V:FITC, 1 x 1.6 ml propidium iodide, 1 x 50 ml vial binding buffer for 100 tests
ANNEX300F Annexin V:FITC Assay Kit, 1 x 1.5 ml annexin V:FITC, 2 x 1.6 ml propidium iodide, 1 x 50 ml vial binding buffer for 300 tests
ANNEX50PE Annexin V:PE Assay Kit, 50 tests annexin V:PE, 7-AAD viability staining solution, 10x binding buffer
ANNEX200PE Annexin V:PE Assay Kit, 200 tests annexin V:PE, 7-AAD viability staining solution, 10x binding buffer
ANNEX50APC Annexin V:APC Assay Kit, 50 tests annexin V:APC, propidium iodide staining solution, 10x binding buffer
ANNEX200APC Annexin V:APC Assay Kit, 200 tests annexin V:APC, propidium iodide staining solution, 10x binding buffer
78.00

66 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

Discontinued products are no longer available.
 Regulated Cell Death Reagents
bio-rad.com/apoptosis Apoptosis Reagents

Caspase Kits

FLICA caspase kits are suitable for cells in suspension and

adherent cells, thin tissue sections, and thin frozen sections
(but not fixed or paraffin-embedded tissues) from many
species, including mammals, insects, and yeast. Various
The following products are available on cell types, including HeLa, primary neurons, macrophages,
bio-rad-antibodies.com. and lymphocytes, have been successfully studied with
Active caspases in apoptotic cells can be analyzed using these kits.
FLICA kits, which offer: FAM FLICA fluoresces green and the SR FLICA fluoresces
■
Unique cell-permeable and non-cytotoxic FLICA reagent red. The latter is useful for studying caspase activity in
GFP-transfected cell lines and for dual-color experiments
■
 uick and easy method suitable for flow cytometry,
Q
with other green reagents. For general apoptosis
fluorescence microscopy, or a plate reader
detection, poly caspase kits (caspase 1, 3, 4, 5, 6, 7, 8, 9) are
■
Choice of 4 fluorophores, from green to far red recommended, as they will react with all active caspases.
FLICA caspase apoptosis detection kits allow analysis Alternatively, individual caspase activity may be studied
of active caspases in whole, living cells. The unique with the single FLICA caspase kits. The Magic Red
cell-permeable and non-cytotoxic reagent, called reagent contains a caspase inhibitor sequence (DEVD)2
fluorochrome inhibitor of caspases (FLICA), contains a linked to a red (cresyl violet) fluorescent probe.
caspase inhibitor sequence (such as VAD) linked to a The FLICA 660 caspase kits use a target sequence
green (carboxyfluorescein, FAM) or red (sulforhodamine, sandwiched between a far red fluorescent 660 dye and a
SR) fluorescent probe. As FLICA binds only to the active fluoromethylketone to make a quick and flexible method to
caspase, there is no interference from pro-caspases or analyze active caspases.
inactive forms of the enzyme. The remaining fluorescent
For More Information
signal gives a direct measure of the amount of active Web: bio-rad.com/apoptosis
caspase in the whole living cell. Cellular analysis may be
performed with a fluorescence microscope, plate reader, or
flow cytometer.

Ordering Information
Catalog # Description Price, U.S. $
ICT097 FAM FLICA Caspase-1 Kit, 25 tests 78.00
ICT098 FAM FLICA Caspase-1 Kit, 100 tests
ICT9122 FLICA 660 Caspase-1 Kit, 25–50 tests
ICT918 FAM FLICA Caspase-2 Kit, 25 tests
ICT093 FAM FLICA Caspase-3/7 Kit, 25 tests
ICT094 FAM FLICA Caspase-3/7 Kit, 100 tests
ICT935 Magic Red Caspase-3/7 Kit, 25 tests
ICT936 Magic Red Caspase-3/7 Kit, 100 tests
ICT9125 FLICA 660 Caspase-3/7 Kit, 25–50 tests
ICT095 FAM FLICA Caspase-6 Kit, 25 tests
ICT096 FAM FLICA Caspase-6 Kit, 100 tests
ICT099 FAM FLICA Caspase-8 Kit, 25 tests
ICT910 FAM FLICA Caspase-8 Kit, 100 tests
ICT912 FAM FLICA Caspase-9 Kit, 25 tests
ICT913 FAM FLICA Caspase-9 Kit, 100 tests
ICT960 SR FLICA Caspase-9 Kit, 25 tests
ICT961 SR FLICA Caspase-9 Kit, 100 tests
ICT923 FAM FLICA Caspase-10 Kit, 100 tests
ICT091 FAM FLICA Poly Caspase Kit, 25 tests
ICT092 FAM FLICA Poly Caspase Kit, 100 tests
ICT916 SR FLICA Poly Caspase Kit, 25 tests
ICT917 SR FLICA Poly Caspase Kit, 100 tests
ICT9120 FLICA 660 Poly Caspase Kit, 25–50 tests

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 67

 Regulated Cell Death Reagents
Apoptosis Reagents bio-rad.com/apoptosis

Mitochondrial Membrane Potential Kits

mitochondria, the dyes aggregate and will fluoresce

bright orange/red. As apoptosis occurs and the
mitochondrial membrane potential collapses, the dyes
disperse throughout the cell and the fluorescence either
The following products are available on diminishes (MitoPT TMRE and TMRM kits) or changes
bio-rad-antibodies.com. color (MitoPT JC-1 kit). This allows an easy distinction
An early indication of apoptosis involves a collapse in between apoptotic and nonapoptotic fluorescent cells,
the electrochemical gradient across the mitochondrial which can be read with a flow cytometer, fluorescence
membrane. This is mediated by the opening of microscope, or a fluorescence plate reader using black
permeability transition (PT) pores and is known as the microtiter plates.
mitochondrial PT event. It is measured by the change ■
 uick and easy staining method clearly differentiates
Q
in the mitochondrial membrane potential. Loss of nonapoptotic and apoptotic cells
mitochondrial membrane potential can be detected by ■
 itoPT TMRE and TMRM kits are ideal for single-cell
M
three fluorescent cationic dyes: 5,5',6,6'-tetrachloro-
multiparametric analyses
1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide
(JC-1), tetramethylrhodamine ethyl ester (TMRE), and ■
 uitable for flow cytometry, fluorescence microscopy, or
S
tetramethylrhodamine methyl ester (TMRM). a plate reader

These dyes are lipophilic and have a delocalized For More Information
Web: bio-rad.com/apoptosis
positive charge, which enables them to enter cells and
negatively charged healthy mitochondria. In nonapoptotic

Ordering Information
Catalog # Description Price, U.S. $
ICT946 Mitochondrial Membrane Potential: MitoPT TMRE Kit, 500 tests 78.00
ICT948 Mitochondrial Membrane Potential: MitoPT TMRM Kit, 500 tests
ICT943 Mitochondrial Permeability Transition: MitoPT JC-1 Kit, 100 tests
ICT944 Mitochondrial Permeability Transition: MitoPT JC-1 Kit, 400 tests

Real-Time Apoptosis Fluorescent Microscopy Kit

■
Ideal for performing time-lapse live cell imaging
experiments
■
Visualize apoptosis events in real time
The following products are available on ■
Perfect for comparing mutant and wild type strains
bio-rad-antibodies.com. ■
Observe transient and reversible apoptotic events
Compatible with conventional fluorescence microscopes ■
 o washing steps or special buffers required — just add
N
and the ZOE fluorescent cell imager, IANBD (excitation the pSIVA probe and start imaging
maximum 488 nm, emission maximum 530 nm) can be ■
Differentiate apoptotic from necrotic/dead cells
easily detected by FITC filter sets. ■
The kit includes the propidium iodide viability dye
For More Information
Web: bio-rad.com/apoptosis

Ordering Information
Catalog # Description Price, U.S. $
APO004 pSIVA Real-Time Apoptosis Fluorescent Microscopy Kit, 1 kit

68 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

 Regulated Cell Death Reagents
bio-rad.com/apoptosis Apoptosis Reagents

Serine Protease Detection Kits

in several different cellular functions, including apoptosis,

tumor malignancy, and as diagnostic and prognostic
indicators of breast, head, and neck carcinomas. Serine
protease activation is also thought to play a role in
The following products are available on infections and transplant rejection. There is increased
bio-rad-antibodies.com. serine protease activity in apoptotic cells downstream of
caspase activation, and this may complement caspase-
FAM FLISP reagents are noncytotoxic, cell permeant green
mediated cell death.
fluorescent inhibitors that covalently bind to active serine
protease enzymes. Simply add FLISP directly to cells in Counterstaining with a viability dye such as propidium
culture, incubate, and wash to detect active chymotrypsin- iodide (contained in the kit) allows identification of necrotic
like enzyme inside the cell. FLISP will covalently bind an cells by flow cytometry. Nuclear morphology may be
active chymotrypsin-like enzyme and be retained within observed by fluorescence microscopy using Hoechst
the cell, whereas unbound reagent will be able to diffuse (contained in the kit).
out. Cells containing lower levels of active enzyme will bind
less FLISP and therefore be less fluorescent after the The kits are available with either a chloromethyl ketone
wash steps. (CMK) or diphenyl 1-(N-peptidylamino) alkanephosphonate
(DAP) reactive group–containing compound with different
Serine proteases, such as trypsin and chymotrypsin, are inhibitor sequences (Phe or Leu) to allow identification of
defined by the presence of a serine residue at the active chymotrypsin or chymotrypsin C.
center of the enzyme, which participates in the formation
For More Information
of an intermediate ester to transiently form an acyl-enzyme Web: bio-rad.com/apoptosis
complex. Activation of serine proteases plays major roles

Ordering Information
Catalog # Description Price, U.S. $
APO006 FLISP FAM-Phe-CMK Serine Protease Assay Kit, 25 tests 78.00
APO007 FLISP FAM-Leu-CMK Serine Protease Assay Kit, 25 tests
APO008 FLISP FAM-Leu-DAP Serine Protease Assay Kit, 25 tests
APO009 FLISP FAM-Phe-DAP Serine Protease Assay Kit, 25 tests

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 69

 Regulated Cell Death Reagents
Apoptosis Reagents bio-rad.com/apoptosis

Apoptosis Antibodies

Visit bio-rad.com/apoptosis for a full list of our apoptosis products.

Ordering Information
Price,
Catalog # Description Clone Species Reactivity Applications Host Formats U.S. $
MCA2712 Annexin V VAA-33 Human E, FC Mouse Pur., Biot., FITC
AHP963 Caspase-1 Polyclonal Human IHC-P,* WB Rabbit Pur. 305.00
AHP1423 Caspase-2 Polyclonal Human, mouse IHC-P,* WB Rabbit Pur. 305.00
AHP2717 Caspase-3 Polyclonal Human, mouse, rat IHC-P, WB Rabbit Pur. 305.00
AHP964 Caspase-4 Polyclonal Human, mouse IHC-P,* WB Rabbit Pur. 305.00
AHP965 Caspase-5 Polyclonal Human WB Rabbit Pur.
AHP1788 Caspase-7 Polyclonal Human, mouse, rat IHC-P,* WB Rabbit Pur.
AHP966 Caspase-7 Polyclonal Human, mouse, rat IHC-P,* WB Rabbit Pur.
MCA2739 Caspase-8 4-1-20 Human E, WB Mouse Pur.
AHP967 Caspase-8 Polyclonal Human, mouse, rat WB Rabbit Pur.
AHP492 Caspase-9 Polyclonal Human, mouse, rat WB Rabbit Pur.
VPA00788 Caspase-10 PrecisionAb Polyclonal Human, mouse WB Rabbit Pur.
AHP626 Caspase-12 Polyclonal Human, mouse, rat IHC-P,* WB Rabbit Pur.
AHP1175 Caspase-14 Polyclonal Human, mouse, rat WB Rabbit Pur.
AHP2286 Caspase-3 Active Polyclonal Human, mouse, rat IHC-P, WB Rabbit Pur.
AHP2288 Caspase-9 Active Polyclonal Human IP, WB Rabbit Pur.
MCA2740 Cytochrome c 2G8 Human E, FC* Mouse Pur.
VMA00017 Bcl-2 PrecisionAb − Human WB Mouse Pur.
VPA00775 Bcl-2 PrecisionAb Polyclonal Human WB Rabbit Pur.
MCA1550 Bcl-2 100 Human FC,* IHC-F, Mouse Pur.
IHC-P,* IP, WB
MCA1539 CD95 LOB 3/17 Human FC, IP Mouse Pur., FITC, RPE
DISC.

AHP2306 CD95 Polyclonal Human, mouse, rat IHC-P, WB Rabbit Pur.

MCA2409 CD178 14C2 Human E, FC, IP Mouse Pur., A488,
A647, RPE
MCA2896 CD178 MFL4 Mouse, rat FC, FN Hamster Pur., A488, A647,
DISC.
FITC, EL, RPE
DISCONTINUED
MCA2408GA CD178 10F2 Human E, FC, FN, IP Mouse Pur., EL 
MCA2118 Granzyme B GB7 Human IHC-P,* WB Mouse Pur.
MCA2119B Granzyme B GB10 Human E Mouse Biot. 305.00
MCA2120 Granzyme B GB11 Human E, FC,* IHC-F, Mouse Pur. 305.00
IF/ICC , IP

Applications: E, ELISA; FC, flow cytometry; FN, functional assays; IF/ICC, immunofluorescence/immunocytochemistry;
IHC-F, immunohistology-frozen; IHC-P, immunohistology-paraffin; IP, immunoprecipitation; WB, western blotting.
Formats: Axxx, Alexa Fluor; Biot., biotin; EL, low endotoxin; FITC, fluorescein isothiocyanate; Pur., purified; RPE, R-phycoerythrin.
* Requires special treatment. Refer to product datasheet.

70 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

Discontinued products are no longer available.
 Regulated Cell Death Reagents
bio-rad.com/autophagy Autophagy Reagents

Autophagy Reagents
Autophagy is the orderly degradation and recycling of cytoplasmic components via the lysosome. It is thought to be a
repair and survival response during stress and infection and has been linked closely to apoptosis. Autophagy can be
studied using antibodies or analysis of active cathepsins. During autophagy, cathepsins hydrolyze and degrade cytoplasmic
proteins, bulk cytoplasm, and dysfunctional organelles for recycling. Increased levels of cathepsin activity have been linked
with conditions such as cancer, arthritis, and Alzheimer’s disease, indicating that cathepsin proteins may be a potential target
for novel therapies.

Cathepsin Detection Kits

The following products are available on L: (z-phenylalanine-arginine)2 easily cross the plasma
bio-rad-antibodies.com. membrane and intracellular organelle membranes of
live cells. Cathepsin activity can be determined by the
Cathepsin detection kits, available in green or red
accumulation of fluorescence when hydrolysis occurs
fluorescence, allow analysis of active cathepsins in
within organelles. An increase in cathepsin activity results
whole, live cells. They use a substrate-based assay with a
in a greater fluorescent signal. Cellular analysis may be
rhodamine 110 or a cresyl violet fluorophore probe. Both
performed with a flow cytometer, fluorescence microscope,
probes are nonfluorescent when bound through amide
or plate reader depending on the kit.
links to two target peptides. Active cathepsins can cleave
the target peptide at an amide link, causing the rhodamine All the kits contain a Hoechst stain for labeling nuclei, and
or mono cresyl violet fluorophores to emit fluorescence. the Magic Red kits contain acridine orange for lysosome
and organelle identification.
Rhodamine 110 emits at 525 nm when excited at 500 nm
and can be detected using microscopy or flow cytometry The cathepsin kits are suitable for cells in suspension,
using the 488 nm laser and a 525/35 nm filter. Cresyl violet adherent cells, and thin frozen tissue sections (but not fixed
emits at 610 nm when excited at 540–590 nm. or paraffin-embedded cells) from many species including
mammalian, insect, and yeast. Various cell types, including
Uncleaved target peptides have excitation and emission
Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7, and
wavelengths of 592 nm and 628 nm respectively, resulting
U937 cells, have also been successfully studied with
in minimal cellular autofluorescence.
these kits.
The photostable substrates for cathepsin B: (z-arginine- For More Information
arginine)2, cathepsin K: (z-leucine-arginine)2 and cathepsin Web: bio-rad.com/autophagy

Ordering Information
Catalog # Description Price, U.S. $
ICT937 Magic Red Cathepsin B Kit, 25 tests 78.00
ICT938 Magic Red Cathepsin B Kit, 100 tests
ICT939 Magic Red Cathepsin K Kit, 25 tests
ICT941 Magic Red Cathepsin L Kit, 25 tests
ICT942 Magic Red Cathepsin L Kit, 100 tests
ICT9151 Green Cathepsin B Kit, 25 tests
ICT9152 Green Cathepsin B Kit, 100 tests 78.00

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 71

 Regulated Cell Death Reagents
Autophagy Reagents bio-rad.com/autophagy

Flow Cytometry Autophagy Assay

Autophagy probe red can be quickly and easily detected

by flow cytometry in cells undergoing autophagy, with
optimal excitation at 590 nm and peak emission at
620 nm (ZE5 cell analyzer settings: 561 nm laser and
The following products are available on 615/24 or 640/20 filter).
bio-rad-antibodies.com.
For More Information
Web: bio-rad.com/autophagy
Autophagy probe red is a cell permeant, aliphatic molecule
that fluoresces brightly when inserted into the lipid
membranes of autophagosomes and autolysosomes.
Autophagy is a dynamic process typically divided into
three stages.

Ordering Information
Catalog # Description Price, U.S. $
APO010A Autophagy Assay Red Detection Kit, 50 tests 78.00
APO010B Autophagy Assay Red Detection Kit, 200 tests

Autophagy Antibodies

Visit bio-rad.com/autophagy for a full list of our autophagy products.

Ordering Information
Catalog # Description Clone Species Reactivity Applications Host Formats Price, U.S. $
AHP1009 Beclin-1 Polyclonal Human, mouse, rat FC, IHC-P,* WB Rabbit Pur. 305.00
AHP2167 MAP1LC3A/B Polyclonal Human IF/ICC, WB Rabbit Pur. 305.00
(N-Terminal)
AHP2448 CD107a (LAMP-1) Polyclonal Mouse, rat IF/ICC, IHC-P, WB Rabbit Pur. 305.00
MCA2881Z CD107a (LAMP-1) B-T47 DISCONTINUED
Mouse FC, IHC-P Mouse Pres. Fr. 305.00
MCA4707T CD107a (LAMP-1) 1D4B Mouse FC, IHC-F, IF/ICC, IP Rat Pur. 305.00
MCA2315GA CD107a (LAMP-1) 4E9/11 Pig FC,* IHC-F, IF/ICC, Mouse Pur., A647, 305.00
IHC-P,* IP, WB FITC
AHP2324 CD107b (LAMP-2) Polyclonal Human, mouse, rat WB Rabbit Pur. 305.00
AHP2325 CD107b (LAMP-2) Polyclonal Human, mouse, rat E, IF/ICC, WB Rabbit Pur.
AHP1634 CD107b (LAMP-2) Polyclonal Human, mouse, rat IHC-F, WB Rabbit Pur.
MCA2293 CD107b (LAMP-2) M3/84 Mouse FC,* IHC-F, IHC-P, Mouse Pur.
IP, WB
MCA2558GA CD107b (LAMP-2) AC17 Dog, human FC,* IF/ICC, IP, WB Mouse Pur., A647,
FITC, RPE

Applications: E, ELISA; FC, flow cytometry; IF/ICC, immunofluorescence/immunocytochemistry;

IHC-F, immunohistology-frozen; IHC-P, immunohistology-paraffin; IP, immunoprecipitation; WB, western blotting.
Formats: Axxx, Alexa Fluor; FITC, fluorescein isothiocyanate; Pres. Fr., preservative free; Pur., purified; RPE, R-phycoerythrin.
* Requires special treatment. Refer to product datasheet.

72 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

Discontinued products are no longer available.
 Regulated Cell Death Reagents
bio-rad.com/pyroptosis Pyroptosis Reagents

Pyroptosis Reagents
Pyroptosis is a form of lytic-regulated cell death triggered by perturbations of extracellular and intracellular homeostasis
in the innate immune system. Its proinflammatory nature distinguishes it from other forms of RCD, such as apoptosis and
necroptosis, and it is in fact the most immunogenic of all the cell death mechanisms. It has distinct morphological features
such as cellular swelling, chromatin condensation, and plasma membrane permeabilization. Caspase-1, activated by various
inflammasomes, cleaves gasdermin D into a 199 amino acid carboxy-terminal fragment and a 242 amino acid amino-terminal
fragment. The amino-terminal of cleaved gasdermin D integrates into the cell membrane to form a pore that directly leads to
cell death.

Pyroptosis Detection Kits

added directly to the cell culture medium, incubated, and

washed. It is cell permeant and will efficiently diffuse in
and out of all cells. If there is an active caspase-1 enzyme
inside the cell, it will covalently bind with YVAD-FMK and
The following products are available on retain the fluorescent signal within the cell. Unbound
bio-rad-antibodies.com. FLICA will diffuse out of the cell during the subsequent
wash steps. Therefore, positive cells will retain a higher
Pyroptosis/Caspase-1 assay kits utilize our popular
concentration of FLICA and fluoresce brighter than
FLICA technology to detect caspase-1 activation. FLICA
negative cells. There is no interference from procaspases
probes are cell permeant noncytotoxic fluorescent labeled
or inactive forms of the enzymes. After labeling with
inhibitors of caspases that covalently bind with active
FLICA, cells can be counterstained with other reagents
caspase enzymes. These kits contain the caspase-1
and fixed or frozen. Cells labeled with YVAD-FMK can be
inhibitor reagent YVAD-FMK, which has the preferred
counterstained with reagents such as the red live/dead
binding sequence for caspase-1, Tyr-Val-Ala-Asp (YVAD).
stains propidium iodide and 7-AAD. Nuclear morphology
This preferred caspase-1 binding sequence is labeled
may be concurrently observed using Hoechst 33342
with either FAM, a green fluorescent dye, or 660, a far
(included in the kit), a blue DNA-binding dye. Cells can
red fluorescent dye, and linked to a fluoromethyl ketone
be viewed through a fluorescence microscope or flow
(FMK) reactive entity. Caspase-1 will not cleave the FLICA
cytometer.
inhibitor probe; instead, it forms an irreversible covalent
bond with the FMK group on the reagent and becomes For More Information
inhibited from further enzymatic activity. FLICA can be Web: bio-rad.com/pyroptosis

Ordering Information
Catalog # Description Price, U.S. $
ICT9145 Pyroptosis FAM Caspase-1 Kit, 25–50 tests 78.00
ICT9146 Pyroptosis FAM Caspase-1 Kit, 100–200 tests
ICT9158 Pyroptosis 660 Caspase-1 Kit, 25 tests

In the U.S., call toll free at 1-800-424-6723 Flow Cytometry | 73

 Flow Cytometry
Subject Index

Subject Index
355 nm Laser Upgrade Kit for ZE5 Cell Analyzer, 44
7 Detectors off 355 nm Laser Upgrade Kit, 44
Accessory Reagents, 61
Annexin V Kits, 65
Apoptosis Antibodies, 70
Autophagy Antibodies, 72
Calibration Reagents, 60
Caspase Kits, 67
Cathepsin Detection Kits, 71
Cell Proliferation Detection Antibodies, 55
Cell Proliferation Assay Reagents, 52
CFDA-SE Cell Proliferation Assay, 54
CytoTrack Cell Proliferation Assay, 53
Everest Software Developer Kit (ZE5 Automation), 45
Flow Cytometry Autophagy Assay, 72
Kits and Reagents for Cell Analysis, 47
Kits and Reagents for Cell Sorting, 40
Large Capacity Fluidics Options, 46
LYNX Conjugation Kits, 50
Mitochondrial Membrane Potential Kits, 68
ProFlow Sort Grade 8x Sheath Fluid, 38
ProFlow Sort Grade Water, 39
ProLine Rainbow Beads, 37
ProLine Universal Calibration Beads, 37
PureBlu DAPI Nuclear Staining Dye, 63
PureBlu Hoechst 33342 Nuclear Staining Dye, 63
Pyroptosis Detection Kits, 73
ReadiDrop Cell Viability Assays, 57
ReadiLink Antibody Labeling Kits, 48
Real-Time Apoptosis Fluorescent Microscopy Kit, 68
Regulated Cell Death, 65
S3e Cell Sorter, 32
S3e Biosafety System Class I, 35
S3e Fluidic Container, 39
S3e Cell Sorter IQ/OQ Services, 36
Serine Protease Detection Kits, 69
Small Particle Detector (SPD) Upgrade Kit, 45
Standards and Stabilizers, 59
VivaFix Cell Viability Assay, 56
ZE5 Cell Analyzer, 41
ZE5 Cell Analyzer IQ/OQ Services, 43

74 | Flow Cytometry In the U.S., call toll free at 1-800-424-6723

Cell Imaging
Cell Imaging 76
 Cell Imaging
bio-rad.com/ZOE

Cell Imaging
Bio-Rad’s suite of tools for fluorescence microscopy and cell imaging includes the ZOE fluorescent cell imager
and nuclear dyes.

See Also ZOE Fluorescent Cell Imager

PureBlu Hoechst
33342 nuclear
staining dye: The ZOE fluorescent cell imager is a complete imaging
page 63.
system that is suitable for routine cell culture and imaging
PureBlu DAPI
nuclear staining dye: applications. It eliminates the complexities of cell imaging
page 63. by combining the ease of use of a personal tablet with the
ReadiLink antibody power of an inverted microscope. An intuitive Android-
labeling kits:
page 48. based touch-screen interface is used to control brightfield,
VivaFix cell three fluorescence channels, and the integrated
viability assays: digital camera.
page 56.
Cell proliferation As a fully integrated system with long-life light-emitting
assays: page 52.
diodes (LEDs), the ZOE cell imager is a robust imaging
device ready for intensive daily use. There are no time-
Video consuming setup and hardware adjustments to perform
Product (optical and illumination alignment, changing filter sets or
Demonstration objective lenses) or parts with a limited life span that could
result in costly maintenance (replacing mercury arc lamps).
The ZOE cell imager uses high-quality hard-coated filter
sets that provide high light transmission and long life.
bio-rad.com/ Its 20x plan achromatic objective lens is mounted in a
ZOEsimplified proprietary manner, resulting in a wide field of view
(0.70 mm2) that is ~180% greater than that of a traditionally
ZOE Fluorescent Cell Imager
mounted 20x objective lens. When zoomed out it is
approximately equivalent to that of a 4x objective.
When needed, use the pinch-to-zoom gesture to magnify
■
 obust construction — fully integrated system with
R
up to 20x by digital zoom while retaining resolution (1 µm). long-life LEDs is ready for intensive daily use
■
 ED light sources — thousands of hours of illumination
L
Product features include: that are instantly ready after power on
■
 implified cell imaging — the intuitive touch-screen
S ■
 arge field of view — the motorized stage and large
L
interface allows users to view cells, capture images, field of view allow you to see more of your sample, faster
and create multichannel merges
■
Small footprint — compact size accommodates
■
Flexible system — brightfield and three fluorescence crowded laboratory benches
channels enable use for routine cell culture applications
as well as more sophisticated imaging applications For More Information
Web: bio-rad.com/ZOE
■
 luorescence at your bench — an integrated light
F Request or download bulletin: 6585
shield permits fluorescence imaging in ambient light

Ordering Information
Catalog # Description Price, U.S. $
1450031 ZOE Fluorescent Cell Imager, 120–240 V, includes instrument, power supply, USB flash drive Inquire

76 | Cell Imaging In the U.S., call toll free at 1-800-424-6723

 Cell Imaging
bio-rad.com/ZOE

Kits and Reagents for Cell Imaging

Bio-Rad offers a variety of kits and reagents for use with the ZOE fluorescent cell imager.

Ordering Information
Catalog # Description Price, U.S. $
Nuclear Staining Dyes
1351303 PureBlu DAPI Nuclear Staining Dye, pkg of 1, includes 5 x 50 µg vials of DAPI powder 105.00
1351304 PureBlu Hoechst 33342 Nuclear Staining Dye, pkg of 1, includes 5 x 56 µg vials of Hoechst 33342 powder 105.00
Antibody Labeling Kits
1351002 ReadiLink 492/516 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer, 310.00
and dyed quench buffer for labeling 2 x 50 µg of antibodies
1351003 ReadiLink 555/570 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer, 310.00
and dyed quench buffer for labeling 2 x 50 µg of antibodies
1351004 ReadiLink 594/610 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer, 310.00
and dyed quench buffer for labeling 2 x 50 µg of antibodies
Fixable Viability Dyes
1351111 VivaFix 353/442 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, 260.00
for running 200 assays
1351115 VivaFix 498/521 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, 260.00
for running 200 assays
1351116 VivaFix 547/573 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, 260.00
for running 200 assays
1351117 VivaFix 583/603 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, 260.00
for running 200 assays

In the U.S., call toll free at 1-800-424-6723 Cell Imaging | 77

 Cell Imaging
Subject Index

Subject Index
Kits and Reagents for Cell Imaging, 77
ZOE Fluorescent Cell Imager, 76

78 | Cell Imaging In the U.S., call toll free at 1-800-424-6723

Chromatography:
Laboratory and
Process Separations
Chromatography Overview 80

Chromatography Media 81
Ion Exchange Chromatography 84
Analytical Grade Ion Exchange Chromatography 90
Mixed-Mode Chromatography 95
Affinity Chromatography 102
Size Exclusion Chromatography 116
Hydrophobic Interaction Chromatography 118
Chromatography Sample Packs, Kits, and Standards 120

Chromatography Columns 124

Prepacked Chromatography Columns 124
Empty Columns 140

Medium-Pressure Chromatography Systems 148

Fraction Collectors 160

Chromatography Accessories 165

Low-Pressure Chromatography Systems 170

Process-Scale Separations 175

 Chromatography Overview
bio-rad.com/chromatography

Chromatography Overview
A Range of Options
Bio-Rad offers a wide selection of chromatography tools for the life scientist involved in analytical, preparative, or process
chromatography. We have become known as a quality provider of chromatography resins for reagent cleanup and
biomolecule purification, manufacturing flexible and intuitive instrumentation and software and prepacked and empty
columns for sample separations.
Throughput, capacity, selectivity, resolution, and process economics are among the considerations when selecting any
chromatography resin, column, or instrument. We offer products for each phase of purification and manufacture resins for
any scale from nanograms to kilograms.
Bio-Rad offers a wide range of lab and process chromatography resins for ion exchange, hydroxyapatite, affinity, size exclusion,
and hydrophobic interaction chromatography as well as chromatography standards. For convenient sample preparation products,
see page 1. Bio-Rad’s process chromatography resins are used worldwide to manufacture registered biotherapeutics and
diagnostics.
Development, Application, and Validation Support
Bio-Rad provides development and application support for large-scale separations. We also offer regulatory assistance and
regulatory support files to help validate the chromatography resins used in your processes. These files contain information essential
to validation, including general product information, specification test procedures, identification tests, and biological safety data.
Learn More about the Technology
Web: bio-rad.com/tech/chrom

Small Molecules, MW <3,000 Nucleic Acids, Proteins, Peptides, MW >3,000

What Molecules
Can Be Purified?

Ion Size Hydrophobic

Exchange Exclusion Interaction

AG Resins, pg. 90 Bio-Beads S-X Resin, Bio-Beads SM-2,

pg. 118 pg. 119
Aminex Columns, pg. 137

Chelex Resins, pg. 90

Bio-Rex Resins, pg. 90

Ion Size Hydrophobic

Mixed-Mode Affinity
Exchange Exclusion Interaction

Nuvia Resin, pg. 84 Nuvia aPrime 4A Resin, Profinity Resin, Bio-Gel P Media, Macro-Prep Methyl
pg. 95
xx pg. 104, 105 pg. 116 Resin, pg. 118
UNOsphere Resin,
pg. 84 Nuvia cPrime Resin, Affi-Gel Resin, Bio-Gel A Media, Macro-Prep t-Butyl
pg. 95 pg. 110 pg. 117 Resin, pg. 118
Macro-Prep Resin,
pg. 87 CHT XT Media, pg. 95
xx. Affi-Prep Resin, Bio-Beads S-X Resin, Bio-Beads SM-2,
pg. 110 pg. 118 pg. 119
UNO Columns, pg. 136 CHT Type I and
Nuvia IMAC Resin, ENrich SEC Columns, EconoFit Columns,
Type II Media, pg. 97 pg. 126 pg. 127
pg. 101
Bio-Rex 70 Resin,
pg. 90 MPC Media, pg. 99
Nuvia IMAC Uncharged Resin, EconoFit Columns,
pg. 101 pg. 127
ENrich IEX Columns, CFT Media, pg. 99
pg. 125
UNOsphere SUPrA,
Bio-Gel HT Media, pg. 109
Foresight Columns, pg. 101
Plates, pg. 130, 131
117, 118 Foresight Columns,
Bio-Gel HTP Media, Plates, pg. 130,
117, 131
118
EconoFit Columns, pg. 101
pg. 127
EconoFit Columns,
Foresight Columns, Plates, pg.127
pg. 130,
117, 118
131

EconoFit Columns,
pg. 127

80 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/chromresin

Chromatography Media
Bio-Rad offers process-scale developers a selection of media for protein separation using ion exchange, hydroxyapatite
and fluoroapatite, affinity, size exclusion (gel filtration), and hydrophobic interaction chromatography. These media offer high
binding capacity, base stability, and high flow rates and are available in multi-liter quantities.
For More Information
Request or download bulletin: 6713

Chromatography Media Selection Guide

Suitability**
Pilot/
Packing Analytical Preparative Process
Media Format* Scale Scale Scale Application Page
Anion Exchange
AG 1 B, GC ++++ ++++ ++++ Strong exchanger. Separation of low MW peptides, 90
nucleotides, inorganic ions using different cross-linkages;
high selectivity for anions such as chloride; gravity or low-
pressure use
AG MP-1M B, GC ++++ ++ + Strong exchanger. Macroporous, equivalent to AG 1 for MW 90
>1,000,000; gravity or low-pressure use
Bio-Rex 5 and B, GC ++++ ++ Weak exchanger. Used to remove organic acids from sugars; 90
AG 4-X4 adsorption of mineral acids; gravity or low-pressure use
UNO Q MC ++++ Strong exchanger. High-resolution biomolecule separation at 136
high flow rates; pH stability 2–12
Macro-Prep High Q B, C, E +++ ++++ +++ Strong exchanger. High-capacity biomolecule separation; 87
and Macro-Prep unique surface chemistry allows contaminant removal;
High Q 3HT pH stability 1–10
Macro-Prep 25 Q B ++++ ++++ + Strong exchanger. Similar to Macro-Prep High Q but 25 µm 87
particle size allows higher-resolution separation; unique
surface chemistry allows contaminant removal; pH stability
1–10
Macro-Prep DEAE B, C, E +++ ++++ +++ Weak exchanger. High-capacity biomolecule separation; 87
unique surface chemistry allows contaminant removal;
pH stability 1–10
UNOsphere Q B, C, E, F ++++ ++++ ++++ Strong exchanger. High-productivity, high-capacity 84
biomolecule separation; pH stability 1–14
Aminex HPLC ++++ High-pressure separation of carbohydrates, sugars, and small 137
organic molecules; delivers industry-standard performance
(U.S. Pharmacopeia)
Nuvia Q B, E, F ++++ ++++ ++++ Strong exchanger. Similar to UNOsphere Q but surface 84
modification allows extremely high capacity biomolecule
separation; pH stability 1–14
Nuvia HP-Q B, E, F ++++ ++++ ++++ Strong anion exchange resin specifically designed for the high- 84
throughput purification of large biomolecules

Cation Exchange
AG 50W B, GC ++++ ++++ ++++ Strong exchanger. Lower cross-linkages useful for peptide 90
and nucleotide separation; higher cross-linkages useful for
small peptide and metals separation and removal of cations;
gravity or low-pressure use
AG MP-50 B, GC ++++ ++ + Strong exchanger. Macroporous equivalent to AG 50W for 90
MW >1,000,000; gravity or low-pressure use

Bio-Rex 70 B ++++ ++ ++ Weak exchanger. High capacity for high MW (>1,000,000) 90

solutes; can be used for purification and fractionation of
peptides, proteins, enzymes, and other cationic molecules.
Amenable to large-scale purification
* B, bottle; C, cartridge (1 or 5 ml); E, EconoFit columns; F, Foresight prepacked plates and columns; GC, gravity column; continues
HPLC, high-pressure column; MC, medium-pressure column; SC, spin column. ** +, low suitability; ++, moderate suitability; +++,
suitable; ++++, high suitability.

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 81
 Chromatography Media
bio-rad.com/chromresin

Chromatography Media Selection Guide

Suitability**
Pilot/
Packing Analytical Preparative Process
Media Format* Scale Scale Scale Application Page
Chelex 100 B, GC ++++ ++++ ++++ Weak exchanger. Chelating resin removes metals and 90
is suitable for PCR applications; can also be used for
ultrapurification of buffers and ionic reagents; gravity or
low-pressure use. Available in molecular biology and
biotechnology grades
UNO S MC ++++ Strong exchanger. High-resolution biomolecule separation 136
at high flow rates; pH stability 2–12
Macro-Prep 25 S B ++++ ++++ + Strong exchanger. Similar to Macro-Prep High S, but 87
25 µm particle size allows higher-resolution separation;
unique surface chemistry allows contaminant removal;
pH stability 1–10
Macro-Prep High S B, C, E +++ ++++ +++ Strong exchanger. High-capacity biomolecule separation; 87
unique surface chemistry allows contaminant removal;
pH stability 1–10
Macro-Prep CM B, E +++ ++++ +++ Weak exchanger. High-capacity biomolecule separation; 87
unique surface chemistry allows contaminant removal;
pH stability 1–10
UNOsphere S B, C, E, F ++++ ++++ ++++  trong exchanger. High-capacity biomolecule separation;
S 84
pH stability 1–14
Nuvia S B, C, E, F ++++ ++++ ++++ Strong exchanger. Similar to UNOsphere S but surface 84
modification allows extremely high capacity biomolecule
separation; pH stability 1–14
Nuvia HR-S B, C, E, F ++++ ++++ ++++ Strong exchanger. Has smaller particle size for high resolution 84
Specialty Ion Exchange
AG 11 A8 B ++++ ++ ++ Ion retardation — contains cation and anion exchange sites 90
that weakly interact with mobile ions; can be used for
desalting of nonionic molecules with water elution, such as
removal of SDS from protein and adsorption of mineral acids
AG 501-X8 B ++++ +++ +++ Mixed bed, consists of equivalent amounts of AG 1-X8 90
and AG 50W-X8. May be used to deionize impure water,
urea, formamide, and acrylamide to provide extremely
pure reagents
Bio-Rex MSZ 501 B ++++ ++ ++ Mixed bed, consists of equivalent amounts of Bio-Rex 90
MSZ 1 and Bio-Rex MSZ 50 resin. Monosized ion
exchange; desalting of water and nonelectrolytes. Ideal for
large-scale industrial applications
Size Exclusion (Gel Filtration
Bio-Gel P B, C, E, SC ++++ ++++ Separation of molecules by size; desalting and buffer 116
exchange; several particle size ranges available with MW
exclusion limits ranging from 100–100,000 D; pH stability 2–10
Bio-Beads S-X B ++++ ++++ ++  ractionation of low MW organic polymers and other
F 118
hydrophobic substances in nonpolar solvents from
400–14,000 D
Affinity
UNOsphere SUPrA B, C, E ++++ +++ Antibody purification; Fc-fusion purification from 109
large volumes of feed/cell culture; development and
commercial-scale mAb purification process applications
Affi-Gel Protein A B, C, GC ++++ ++++ IgG purification from ascites, serum, and culture fluid; 110
low-pressure resin
Affi-Prep Protein A B, C, E ++++ ++++ ++ IgG purification from ascites, serum, and culture fluid; 110
pressure-stable resin
Affi-Gel Blue B, C, E, SC ++++ ++++  lbumin removal and enzyme purification; Cibacron Blue
A 111
F3GA dye covalently attached; purification of proteins
with dinucleotide fold
continues

82 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/chromresin

Chromatography Media Selection Guide

Suitability**
Pilot/
Packing Analytical Preparative Process
Media Format* Scale Scale Scale Application Page
DEAE Affi-Gel Blue B, C, E, GC ++++ ++++ Albumin and protease removal for IgG purification; Cibacron 111
Blue F3GA dye covalently attached to DEAE Bio-Gel A
CM Affi-Gel Blue B ++++ ++++  lbumin and protease removal for IgG purification; Cibacron
A 112
Blue F3GA dye covalently attached to CM Bio-Gel A
Affi-Gel heparin B ++++ ++++  urification of coagulation factors, plasma proteins, and
P 114
enzymes including nucleases, lipases, and proteases;
binding specific to a variety of enzymes and other proteins
Nuvia IMAC B, C, E ++ ++++ ++++ Purification of recombinant histidine-tagged proteins; 102
can be charged with other transition metals
Nuvia IMAC, B, C, E ++ ++++ ++ Purification of recombinant histidine-tagged proteins; 102
uncharged can be charged with other transition metals
Profinity IMAC B, C, E ++++ ++++ ++ Histidine-tagged protein purification 104
Profinity GST C, E ++++ ++++ ++ GST-tagged protein purification 105
Profinity eXact B, C, E, SC ++++ ++++ ++ One-step affinity tag purification and on-column cleavage 106
Affi-Prep polymyxin B ++++ ++++ ++++ Removal of endotoxins; pressure-stable resin capable 113
of sanitization procedures with NaOH
Affi-Gel boronate B ++++ ++  ffinity for low MW molecules containing cis hydroxyl
A 113
(cis-diol) groups; separation of AMP from cyclic AMP
Profinity epoxide B ++++ ++++ ++++  ffinity coupling; coupling of nucleophiles such as hydroxy
A 105
(–OH), amino (–NH2), or thiol (–SH) groups; based on
UNOsphere base matrix for superb pressure flow
characteristics
Affi-Gel 10 B ++++ ++++ Affinity coupling; immobilization of ligands with –NH2 groups, 114
coupling of proteins with pl 6.5–11; low-pressure resin
Affi-Gel 15 B ++++ ++++  ffinity coupling; immobilization of ligands with
A 114
–NH2 groups, coupling of proteins with pl <6.5;
low-pressure resin
Affi-Gel Hz B ++++ ++++ Affinity coupling; immobilization of IgG molecules via 115
their Fc region
Affi-Gel 102 B ++++ ++++ Affinity coupling of ligands with –COOH groups via EDAC 115
coupling chemistry
Mixed-Mode
Nuvia aPrime 4A B, E, F, ++++ ++++ ++++ Biomolecule purification, a hydrophobic anion exchange resin 95
Nuvia cPrime B, E, F ++++ ++++ ++++ Biomolecule purification, a hydrophobic cation exchange resin 95
CHT XT B, E, F ++++ ++++ ++++ Antibody purification; virus purification/removal; DNA 97
purification/removal; aggregate and endotoxin removal
CHT Type I B, C, ++++ ++++ ++++ Antibody purification (high capacity); virus purification/ 97
MC, E, F removal; DNA purification/removal; aggregate and
endotoxin removal
CHT Type II B, C, E, F ++++ ++++ ++++  ntibody purification; removal of albumin from feedstream;
A 97
vaccine/VLP purification
MPC Type I B, E, F ++++ ++++ ++++ Antibody purification; virus purification/removal; DNA 99
purification/removal; aggregate and endotoxin removal
CFT Type II B, E ++++ ++++ ++++ Similar properties to CHT but exhibits greater stability in the 99
lower pH range (5.5)
Bio-Gel HT B ++++ +++ Purification of proteins, nucleic acids, and other 101
biomolecules; crystalline hydroxyapatite
Bio-Gel HTP B ++++ +++ Similar to Bio-Gel HT but in powder form 101
DNA grade B ++++ +++ Similar to Bio-Gel HTP with smaller particle size; selectivity 101
Bio-Gel HTP for double-stranded DNA; single- and double-stranded
DNA separation
continues

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 83
 Chromatography Media
Ion Exchange Chromatography bio-rad.com/ionexchange

Chromatography Media Selection Guide

Suitability**
Pilot/
Packing Analytical Preparative Process
Media Format* Scale Scale Scale Application Page
Hydrophobic Interaction
Macro-Prep methyl B, E ++++ ++++ ++++ Separation of proteins based on relative hydrophobicity; 118
pH stability 1–10
Macro-Prep t-butyl B, E ++++ ++++ ++++ Separation of proteins based on relative hydrophobicity; 118
pH stability 1–10
Bio-Beads SM-2 B ++++ ++++ ++++ Useful for the adsorption of nonpolar substances or surface 119
active agents from aqueous solutions
*B
 , bottle; C, cartridge (1 or 5 ml); E, EconoFit columns; F, Foresight prepacked plates and columns; GC, gravity column; HPLC, high-pressure
column; MC, medium-pressure column; SC, spin column. ** +, low suitability; ++, moderate suitability; +++, suitable; ++++, high suitability.

Ion Exchange Chromatography

See Also UNOsphere and Nuvia Ion Exchange Media

Chromatography
systems:
page 148. Bio-Rad’s ion exchange resins are scalable and fast. They ■
Use less resin to purify a given amount of product
Prepacked columns: are designed to meet the needs of the biopharmaceutical ■
 educe cycle time and increase productivity by operating
R
page 124.
industry for capture, intermediate, and polishing stages of at higher flow rates
Ion exchange
chromatography purification. UNOsphere and Nuvia resins are bioprocess ■
 educe cost and space requirements by decreasing
R
standards: compatible and may also be used at laboratory scales for
page 122. buffer consumption
high-performance applications. Benefits include:
Sample preparation ■
 educe capital and operating expenses by using
R
products: page 1. ■
Efficient capture from crude feedstreams
Resin sampler smaller columns
packs: page 120.
■
Optimization to operate under 2 bar at 1,200 cm/hr ■
Chemical stability for repetitive clean-in-place cycles
Bio-Scale Mini ■
 arge-pore design results in ultra-high binding capacities
L
cartridges: ■
Flexibility for capture or polish steps
page 132. at fast linear velocities
■
Fully supported for regulatory submission
EconoFit columns ■
Fully supported for regulatory information
and kits:
page 127 Nuvia HP-Q Resin
UNOsphere Q and S Resins
Nuvia HP-Q is a strong anion exchange resin specifically
UNOsphere Q and S resins are strong anion and cation
designed for the high-throughput purification of large
exchange resins, respectively, and may be used at any
biomolecules. The large pores, optimized with surface
stage of the purification process.
extenders, together with the rigid base beads provide
For More Information fast mass transfer kinetics at high flow rates for large
Web: bio-rad.com/unosphere
biomolecule purification.
Download bulletins: UNOsphere Q resin — 2724 and 2729;
UNOsphere S resin — 2669 and 2678 ■
 ptimized surface extender/ligand density allows high
O
Nuvia Q, S, and HR-S Resins dynamic binding capacity
Nuvia ion exchange resins are a family of next-generation ■
Favorable pressure flow characteristics at scale
ion exchange products built on an industry-proven ■
Base stability for excellent process economics
proprietary base matrix technology. Nuvia resins provide
very high capture and exceptional flow properties designed For More Information
Web: bio-rad.com/nuvia
to meet current and future process needs. Nuvia Q and S Download bulletin: 5984
are flexible alternatives that may be used as capture Request or download bulletins: 5987, 6128, 6129, 6241, and 6448
and/or polishing solutions. Nuvia HR-S is a high-resolution
cation exchanger designed for intermediate and final
polish applications.

84 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/ionexchange Ion Exchange Chromatography

Properties of UNOsphere and Nuvia

Property UNOsphere Q UNOsphere S Nuvia Q Nuvia S Nuvia HR-S Nuvia HP-Q
Type of ion exchanger Strong anion Strong cation Strong anion Strong cation Strong cation Strong anion
Functional group –N+(CH3)3 –SO3– –N(CH3)3+ –SO3– –SO3– –N(CH3)3+
Total ionic capacity 120 µeq/ml 269 ± 50 µeq/ml 100–170 µeq/ml 90–150 µeq/ml 100–180 µeq/ml 48–88 µeq/ml
Median particle size, μm 120 80 85 85 50 50
Dynamic binding capacity*,**
At 100 cm/hr 180 mg BSA/ml 60 mg IgG/ml — — — >50 mg/ml at 100 cm/hr**
At 300 cm/hr — — >170 mg/ml >110 mg/ml ≥70 mg/ml —
At 600 cm/hr 125 mg BSA/ml 30 mg IgG/ml — — — —
Recommended linear 50–300 cm/hr 50–300 cm/hr 50–600 cm/hr 50–300 cm/hr 50–200 cm/hr 50–300 cm/hr
flow rate range
pH stability 1–14 1–14 2–14 short term 2–14 short term 2–14 short term 2–13 short term
(accelerated, 60°C)
— — 4–12 long term 4–13 long term 4–13 long term 2–10 long term
Sanitization 0.5–1.0 N NaOH 0.5–1.0 N NaOH 0.5–1.0 N NaOH 0.5–1.0 N NaOH 0.5–1.0 N NaOH 0.5 N NaOH
* 10% breakthrough capacity determined with a 5.0 mg/ml human IgG and 5.0 mg/ml BSA in a 1.1 x 20 cm column.
** Nuvia HP-Q 10% breakthrough capacity determined with 1.1 mg/ml thyroglobulin in a 0.7 x 5.5 cm column.

Ordering Information
Catalog # Description U.S. $
1560311 Nuvia S Media, 25 ml Ultra high capacity strong cation process media 105.06
1560313 Nuvia S Media, 100 ml 315.18
156-0315 Nuvia S Media, 500 ml 1,163.01
156-0317 Nuvia S Media, 10 L Inquire
1560511 Nuvia HR-S Media, 25 ml High-resolution strong cation process media 99.81
1560513 Nuvia HR-S Media, 100 ml 327.79
156-0515 Nuvia HR-S Media, 500 ml 1,365.78
156-0517 Nuvia HR-S Media, 10 L Inquire
1560411 Nuvia Q Media, 25 ml Ultra high capacity strong anion process media 106.09
1560413 Nuvia Q Media, 100 ml 315.18
156-0415 Nuvia Q Media, 500 m l 1,163.01
156-0417 Nuvia Q Media, 10 L Inquire
12006693 Nuvia HP-Q Media, 25 ml High-performance strong anion process media 92.00
12006691 Nuvia HP-Q Media, 100 ml
12006660 Nuvia HP-Q Media, 500 ml
12006659 Nuvia HP-Q Media, 5 L
12007023 Nuvia HP-Q Media, 10 L
1560101 UNOsphere Q Media, 25 ml High-capacity strong anion media 92.00
1560103 UNOsphere Q Media, 100 ml 285.00
156-0105 UNOsphere Q Media, 500 ml 1,046.96
156-0107 UNOsphere Q Media, 10 L Inquire
1560111 UNOsphere S Media, 25 ml High-capacity strong cation media 92.00
1560113 UNOsphere S Media, 100 ml 285.00
156-0115 UNOsphere S Media, 500 ml 1,046.96
156-0117 UNOsphere S Media, 10 L Inquire

Description 1 x 1 ml Price, U.S. $ 5 x 1 ml Price, U.S. $ 1 x 5 ml Price, U.S. $ 5 x 5 ml

Prepacked Bio-Scale Mini Cartridges
Nuvia S Media 7324420 69.00 7324421 215.00 7324422 180.00 7324423 700.00
UNOsphere Q Media —
DISCONTINUED
7324100 210.00 7324102 150.00 7324104 505.00
UNOsphere S Media — 7324110 210.00 7324112 150.00 7324114 505.00
continues

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 85
Discontinued products are no longer available.
 Chromatography Media
Ion Exchange Chromatography bio-rad.com/ionexchange

Ordering Information
Catalog # Description U.S. $
Foresight Plates*
732-4714  oresight UNOsphere Q, 20 μl
F 551.25
732-4710 F  oresight UNOsphere S, 20 μl 551.25
732-4703 F  oresight Nuvia Q, 20 μl 551.25
732-4701 F  oresight Nuvia S, 20 μl 551.25
732-4707 Foresight Nuvia HR-S, 20 μl 540.75
12006908 F  oresight Nuvia HP-Q, 20 μl 551.25

Description Catalog # Catalog #

Foresight Columns 1 ml Price, U.S. $ 5 ml Price, U.S. $
Foresight UNOsphere Q 732-4732 104.25 732-4752 192.14
Foresight UNOsphere S 732-4730 104.25 732-4750 192.14
Foresight Nuvia Q 732-4721 115.83 732-4741 213.49
Foresight Nuvia S 732-4720 115.83 732-4740 213.49
Foresight Nuvia HR-S 732-4723 126.79 732-4743 231.53
Foresight Nuvia HP-Q 12007020 126.79 12007021 231.53
Foresight RoboColumn Units** 200 μl Price, U.S. $ 600 μl Price, U.S. $
Foresight UNOsphere Q 732-4819 192.94 732-4820 231.53
Foresight UNOsphere S 732-4813 192.94 732-4814 231.53
Foresight Nuvia Q 732-4804 212.78 732-4805 254.68
Foresight Nuvia S 732-4801 212.23 732-4802 254.68
Foresight Nuvia HR-S 732-4831 203.96 732-4832 259.09
Foresight Nuvia HP-Q 12007013 126.79 12007014 259.09
Catalog # Description U.S. $
EconoFit Prepacked Ion Exchange Columns
12009307 EconoFit UNOsphere Q Column, 1 x 1 ml , 7 mm x 25 mm
12009302 EconoFit UNOsphere Q Column, 1 x 5 ml, 16 mm x 25 mm
12009301 EconoFit UNOsphere Q Columns, 5 x 1 ml, 7 mm x 25 mm
12009303 EconoFit UNOsphere Q Columns, 5 x 5 ml, 16 mm x 25 mm
12009308 EconoFit UNOsphere S Column, 1 x 1 ml, 7 mm x 25 mm
12009305 EconoFit UNOsphere S Column, 1 x 5 ml, 16 mm x 25 mm
12009304 EconoFit UNOsphere S Columns, 5 x 1 ml, 7 mm x 25 mm
12009306 EconoFit UNOsphere S Columns, 5 x 5 ml, 16 mm x 25 mm
12009290 EconoFit Nuvia Q Column, 1 x 1 ml, 7 mm x 25 mm
12009291 EconoFit Nuvia S Column, 1 x 1 ml, 7 mm x 25 mm
12009284 EconoFit Nuvia HR-S Column, 1 x 1 ml, 7 mm x 25 mm
12009282 EconoFit Nuvia HP-Q Column, 1 x 1 ml, 7 mm x 25 mm
EconoFit Prepacked Column Kits
12009311 EconoFit Anionic IEX Kit, includes 1 ml each of Macro-Prep High Q, Macro-Prep High Q-3HT, UNOsphere Q, Nuvia Q, 145.00
Macro-Prep DEAE, and Nuvia HP-Q DISCONTINUED
12009310 EconoFit Cationic IEX Kit, includes 1 ml each of Macro-Prep High S, UNOsphere S, Nuvia S, Nuvia HR-S, 97.00
and Macro-Prep CM
Adaptor Fittings for Bio-Scale Mini Cartridges
7320111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system 145.00
7320112 Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC or NGC system 97.00
7320113 Luer to BioLogic and NGC Systems Fittings Kit, low-pressure system fittings kit, includes 1/4–28 female to male and 59.00
1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow and NGC systems
7885010 Luer to 10-32 Adaptor Fittings Kit, includes female slip luer to female 10-32 fitting to connect male end of luer column 175.00
to NGC system
* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.

86 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Discontinued products are no longer available.
 Chromatography Media
bio-rad.com/ionexchange Ion Exchange Chromatography

Macro-Prep Ion Exchange Media See Also

Ion exchange
chromatography
Macro-Prep ion exchange resins are designed to provide standards: page 122.
high resolution and high capacity for preparative
separations. The rigid methacrylate beads exhibit little
shrinkage or swelling, making them suitable for both low-
and medium-pressure chromatography. The macroporous
resin allows both small and large molecules to access
exchange sites located throughout the chromatography
bed. The physical structure of the resin permits high
flow rates at low backpressure. Depending on the resin,
pH conditions, and samples, the products can display
Macro-Prep Resins Bio-Scale Mini Macro-Prep Cartridges
mixed-mode behavior.
Benefits include: Macro-Prep 25 Q and S Resins
■
High capacity for biomolecules Macro-Prep 25 Q strong anion exchange resin and
■
High resolution of complex biological mixtures Macro-Prep 25 S strong cation exchange resin offer
high-resolution separations at high flow rates with
■
Rigid methacrylate polymer matrix that allows high flow medium pressures. Both resins possess the same rigid,
rates at modest pressures macroporous, and hydrophilic properties of Macro-Prep
Macro-Prep High Q, High Q-3HT, DEAE, High S, High Q and High S resins, but in a 25 μm bead that offers
and CM Resins higher resolution.
For maximum flexibility, the product offerings include For More Information
Macro-Prep High Q strong anion exchange resin, Web: bio-rad.com/macroprepSandQ
Macro-Prep DEAE weak anion exchange resin, Download bulletin: 2292
Macro-Prep High S strong cation exchange resin,
and Macro-Prep CM weak cation exchange resin.
For More Information
Web: bio-rad.com/macroprep
Download bulletins: Macro-Prep DEAE resin — 1942;
High S and High Q resins — 5643 and 5644

Properties of Macro-Prep
Property High Q High Q-3HT DEAE High S CM 25 Q* 25 S
Type of resin Strong anion Strong anion Weak anion Strong cation Weak cation Strong anion Strong cation
Functional ligand –N+ (CH3)3 –N+ (CH3)3 –N+ (C2H5)2 –SO3– –COO– –N+(CH3)3 –SO3–
Ionic capacity 400 ± 75 µeq/ml 475 ± 45 µeq/ml 175 ± 75 µeq/ml 160 ± 40 µeq/ml 210 ± 40 µeq/ml 220 ± 70 µeq/ml 110 ± 30 µeq/ml
≥25 mg
Typical binding capacity ≥37 mg BSA/ml ≥37 mg BSA/ml ≥30 mg BSA/ml ≥49 mg hIgG/ml ≥30 mg BSA/ml ≥35 mg hIgG/ml
hemoglobin/ml
Mean particle size** 50 µm 50 µm 50 µm 50 µm 50 µm 25 µm 25 µm
Recommended linear
50–300 cm/hr 50–300 cm/hr 50–300 cm/hr 50–300 cm/hr 50–300 cm/hr 50–300 cm/hr 50–300 cm/hr
flow rate range
pH stability 1–10*** 1–10*** 1–10*** 1–10*** 1–12*** 1–10*** 1–10***
Storage conditions 20% ethanol 20% ethanol 20% ethanol 20% ethanol 20% ethanol 20% ethanol 20% ethanol
* Do not autoclave the OH– form.
** Mean particle size measured before derivatization.
*** The use of basic reagents greater than pH 10 should be evaluated for each application.

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 87
 Chromatography Media
Ion Exchange Chromatography bio-rad.com/ionexchange

Ordering Information
Catalog # Description Price, U.S. $
Macro-Prep High Q Media
1580040 Macro-Prep High Q Media, 25 ml High-capacity strong anion exchange media; 48.00
1560040 Macro-Prep High Q Media, 100 ml very high flow rates and resolution 155.00
156-0041 Macro-Prep High Q Media, 500 ml 612.00
156-0042 Macro-Prep High Q Media, 5 L Inquire
156-0043 Macro-Prep High Q Media, 10 L Inquire
Macro-Prep High Q-3HT Media
1560040-3HT Macro-Prep High Q-3HT Media, 100 ml High-capacity strong anion exchange media; 48.00
1560041-3HT Macro-Prep High Q-3HT Media, 500 ml very high flow rates and resolution 155.00
1560042-3HT Macro-Prep High Q-3HT Media, 5 L 612.00
1560043-3HT Macro-Prep High Q-3HT Media, 10 L Inquire
Macro-Prep DEAE Media
1580020 Macro-Prep DEAE Media, 25 ml High-capacity weak anion exchange media; 48.00
1560020 Macro-Prep DEAE Media, 100 ml very high flow rates and resolution 155.00
156-0021 Macro-Prep DEAE Media, 500 ml 612.00
156-0022 Macro-Prep DEAE Media, 5 L Inquire
156-0023 Macro-Prep DEAE Media, 10 L Inquire
Macro-Prep High S Media
1580030 Macro-Prep High S Media, 25 ml High-capacity strong cation exchange media; 48.00
1560030 Macro-Prep High S Media, 100 ml very high flow rates and resolution 155.00
156-0031 Macro-Prep High S Media, 500 ml Inquire
156-0032 Macro-Prep High S Media, 5 L Inquire
156-0033 Macro-Prep High S Media, 10 L Inquire
Macro-Prep CM Media
1580070 Macro-Prep CM Media, 25 ml High-capacity weak cation exchange media; 56.00
1560070 Macro-Prep CM Media, 100 ml high flow rates and resolution 170.00
156-0071 Macro-Prep CM Media, 500 ml Inquire
156-0073 Macro-Prep CM Media, 10 L Inquire
Macro-Prep 25 Q Media
1530021 Macro-Prep 25 Q Media, 50 ml Strong anion exchange media; 25 µm bead that offers 655.00
1530022 DISCONTINUED
Macro-Prep 25 Q Media, 200 ml higher resolution 2,355.00
153-0023 Macro-Prep 25 Q Media, 1 L Inquire
153-0024 Macro-Prep 25 Q Media, 5 L Inquire
Macro-Prep 25 S Media
1530031 Macro-Prep 25 S Media, 50 ml Strong cation exchange media; 25 µm bead that offers 655.00
1530032 DISCONTINUED
Macro-Prep 25 S Media, 200 ml higher resolution 2,300.00
153-0033 Macro-Prep 25 S Media, 1 L Inquire
153-0034 Macro-Prep 25 S Media, 5 L Inquire
Catalog # Description.S. $
EconoFit Macro-Prep Columns*
12009273 EconoFit Macro-Prep CM Column, 1 x 1 ml, 7 x 25 mm
12009274 EconoFit Macro-Prep DEAE Column, 1 x 1 ml, 7 x 25 mm
12009265 EconoFit Macro-Prep DEAE Column, 1 x 5 ml, 16 x 25 mm
12009264 EconoFit Macro-Prep DEAE Columns, 5 x 1 ml, 7 x 25 mm
12009266 EconoFit Macro-Prep DEAE Columns, 5 x 5 ml, 16 x 25 mm
12009275 EconoFit Macro-Prep High Q Column, 1 x 1 ml, 7 x 25 mm
12009268 EconoFit Macro-Prep High Q Column, 1 x 5 ml, 16 x 25 mm
12009267 EconoFit Macro-Prep High Q Columns, 5 x 1 ml, 7 x 25 mm
12009269 EconoFit Macro-Prep High Q Columns, 5 x 5 ml, 16 x 25 mm
12009283 EconoFit Macro-Prep High Q-3HT Column, 1 x 1 ml, 7 x 25 mm
12009276 EconoFit Macro-Prep High S Column, 1 x 1 ml, 7 x 25 mm
12009271 EconoFit Macro-Prep High S Column, 1 x 5 ml, 16 x 25 mm
12009270 EconoFit Macro-Prep High S Columns, 5 x 1 ml, 7 x 25 mm
12009272 EconoFit Macro-Prep High S Columns, 5 x 5 ml, 16 x 25 mm
12009277 EconoFit Macro-Prep Methyl HIC Column, 1 x 1 ml, 7 x 25 mm
12009278 EconoFit Macro-Prep t-Butyl HIC Column, 1 x 1 ml, 7 x 25 mm
* Maximum pressure 72 psi  continues

88 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Discontinued products are no longer available.
 Chromatography Media
bio-rad.com/ionexchange Ion Exchange Chromatography

Ordering Information
Catalog # Description.S. $
EconoFit Prepacked Column Kits
12009311 EconoFit Anionic IEX Kit, includes 1 ml each of Macro-Prep High Q, Macro-Prep High Q-3HT, UNOsphere Q, Nuvia Q, Macro-Prep
DEAE, and Nuvia HP-Q
12009310 EconoFit Cationic IEX Kit, includes 1 ml each of Macro-Prep High S, UNOsphere S, Nuvia S, Nuvia HR-S, and Macro-Prep CM

Description 5DISCONTINUED
x 1 ml Price, U.S. $ 1 x 5 ml Price, U.S. $ 5 x 5 ml Price, U.S. $
Prepacked Bio-Scale Mini Cartridges
Macro-Prep High Q Media 7324120 210.00 7324122 80.00 7324124 290.00
Macro-Prep High S Media 7324130 210.00 7324132 80.00 7324134 290.00
Macro-Prep DEAE Media 7324140 235.00 7324142 80.00 7324144 300.00
Catalog # Description Price, U.S. $
Adaptor Fittings for Bio-Scale Mini Cartridges
7320111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system 48.00
7320112 Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC 155.00
or NGC system
7320113 Luer to BioLogic and NGC Systems Fittings Kit, low-pressure system fittings kit, includes 1/4–28 female
to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow and NGC systems
7885010 Luer to 10-32 Adaptor Fittings Kit, includes female slip luer to female 10-32 fitting to connect male Inquire
end of luer column to NGC system
Larger volumes and special packaging for industrial applications are available on request.

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 89
Discontinued products are no longer available.
 Chromatography Media
Analytical Grade Ion Exchange Chromatography bio-rad.com/agresins

Analytical Grade Ion Exchange Chromatography

AG, Bio-Rex, and Chelex Resins

AG (analytical grade) resins — AG resins are primarily ■

AG 501-X8 mixed-bed resins — useful for deionization
used for the separation of low MW compounds such of water and nonelectrolyte solutions
as inorganic ions, organic acids, nucleic acids, or Bio-Rex resin — available as weak anion and
carbohydrates. They are available as both strong and cation exchangers and as monosized mixed-bed ion
weak cation and anion exchangers and as mixed-bed ion exchangers. Bio-Rex 70 resins are macroreticular
exchangers. Many are available in several ionic forms and resins with a high capacity for high molecular weight
can be converted from one form to another compounds used for the purification and fractionation
■
AG 1-X8 strong anion exchanger resins — useful for of proteins and peptides.
sample preparation, enzyme assays, metal separations, Chelex resins — contain paired iminodiacetate ions
and peptide, protein, and nucleic acid separations coupled to a styrene divinylbenzene support. They are
■
 G 50W-X8 strong cation exchanger resins —
A unique chelating resins that bind polyvalent cations with
particularly useful for the removal of ethidium bromide high selectivity and are used to remove metal ions from
and propidium iodide from DNA samples samples and buffers.

Analytical Grade Resin Wet Mesh and Equivalent Diameters

Wet mesh (U.S. standard) 16 20 40 50 80 100 140 200 270 325 400
Diameter, µm 1,180 850 425 300 180 150 106 75 53 45 38

For More Information

Web: bio-rad.com/agresins
Request or download bullein: 6904

90 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/agresins Analytical Grade Ion Exchange Chromatography

See Also
Ordering Information
Nominal Poly-Prep ion
Dry Mesh Wet Bead Shipping % exchange columns:
page 141.
Catalog # Description Ionic Form Size Size, µm Water Price, U.S. $
AG Resins
140-1231 AG 1-X2 Resin, 500 g Chloride 50–100 180–500 70­–78 625.00
140-1241 AG 1-X2 Resin, 500 g Chloride 100–200 106–250 70­–78 505.00
140-1251 AG 1-X2 Resin, 500 g Chloride 200–400 75–180 70­–78 505.00
140-1253 AG 1-X2 Resin, 500 g Acetate 200–400 75–180 70­–78 775.00
140-1331 AG 1-X4 Resin, 500 g Chloride 50–100 180–425 59­– 65 460.00
140-1341 AG 1-X4 Resin, 500 g Chloride 100–200 106–250 59­– 65 625.00
140-1351 AG 1-X4 Resin, 500 g Chloride 200–400 63–150 59­– 65 695.00
140-1421 AG 1-X8 Resin, 500 g Chloride 20–50 300–1,180 39­–45 355.00
140-1431 AG 1-X8 Resin, 500 g Chloride 50–100 180–425 39­–45 545.00
140-1441* AG 1-X8 Resin, 500 g Chloride 100–200 106–180 39­–45 545.00
140-1451* AG 1-X8 Resin, 500 g Chloride 200–400 45–106 39­–45 585.00
140-1422 AG 1-X8 Resin, 500 g Hydroxide 20–50 300–1,180 39­–45 440.00
140-1443 AG 1-X8 Resin, 500 g Acetate 100–200 106–180 39­–45 695.00
140-1453 AG 1-X8 Resin, 500 g Acetate 200–400 45–106 39­–45 595.00
140-1444 AG 1-X8 Resin, 500 g Formate 100–200 106–180 39­–45 595.00
140-1454 AG 1-X8 Resin, 500 g Formate 200–400 45–106 39­–45 595.00
142-1231 AG 50W-X2 Resin, 500 g Hydrogen 50–100 300–1,180 75­–83 1,050.00
142-1241* AG 50W-X2 Resin, 500 g Hydrogen 100–200 106–300 75­–83 730.00
142-1251 AG 50W-X2 Resin, 500 g Hydrogen 200–400 75–180 75­–83 1,290.00
142-1331 AG 50W-X4 Resin, 500 g Hydrogen 50–100 180–425 64–72 580.00
142-1341 AG 50W-X4 Resin, 500 g Hydrogen 100–200 106–250 64–72 590.00
142-1351* AG 50W-X4 Resin, 500 g Hydrogen 200–400 63–150 64–72 560.00
142-1421 AG 50W-X8 Resin, 500 g Hydrogen 20–50 300–1,180 50–56 460.00
142-1431 AG 50W-X8 Resin, 500 g Hydrogen 50–100 180–425 50–56 730.00
142-1441*,** AG 50W-X8 Resin, 500 g Hydrogen 100–200 106–250 50–56 620.00
142-1451*,** AG 50W-X8 Resin, 500 g Hydrogen 200–400 63–150 50–56 735.00
142-1641 AG 50W-X12 Resin, 500 g Hydrogen 100–200 106–250 42–48 1,295.00
142-1651 AG 50W-X12 Resin, 500 g Hydrogen 200–400 53–106 42–48 2,060.00
141-1831 AG MP-1M Resin, 500 g Chloride 50–100 150–300 56–64 745.00
141-1841 AG MP-1M Resin, 500 g Chloride 100–200 75–150 56–64 795.00
141-1851 AG MP-1M Resin, 500 g Chloride 200–400 38–75 56–64 1,075.00
143-0841 AG MP-50 Resin, 500 g Hydrogen 100–200 75–150 46–52 865.00
140-4341* AG 4-X4 Resin, 500 g Free base 100–200 75–150 — 545.00
1437424* AG 501-X8 Resin, 100 g H+ + OH– 20–50 300–1,180 43–55 545.00
142-6424*,**,*** AG 501-X8 Resin, 500 g H+ + OH– 20–50 300–1,180 43–55 360.00
142-6425*,** AG 501-X8(D) Resin, 500 g H+ + OH– 20–50 300–1,180 43–55 510.00
142-7834* AG 11 A8 Resin, 500 g Self-adsorbed 50–100 180–425 — 560.00
Bio-Rex Resins
140-7841 Bio-Rex 5 Resin, 500 g Chloride 100–200 75–150 50–58 975.00
140-7851 Bio-Rex 5 Resin, 500 g Chloride 200–400 45–75 50–58 1,510.00
142-5822 Bio-Rex 70 Resin, 500 g Sodium 20–50 300–1,180 65–74 795.00
142-5832* Bio-Rex 70 Resin, 500 g Sodium 50–100 150–300 65–74 795.00
142-5842 Bio-Rex 70 Resin, 500 g Sodium 100–200 75–150 65–74 795.00
142-5852* Bio-Rex 70 Resin, 500 g Sodium 200–400 45–75 65–74 795.00
142-7425* Bio-Rex MSZ 501(D) H+ + OH– 25–35 500–700 — 435.00
Resin, 500 g
142-7426* Bio-Rex MSZ 501(D) H+ + OH– 25–35 500–700 — 435.00
Resin, 10 kg
* Also available as biotechnology grade resin.
** Also available as molecular biology grade resin.
*** Also available as reactor grade resin. continues

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 91
 Chromatography Media
Analytical Grade Ion Exchange Chromatography bio-rad.com/agresins

See Also
Ordering Information
Ion exchange
chromatography Nominal
standards: Dry Mesh Wet Bead Shipping %
page 122. Catalog # Description Ionic Form Size Size, µm Water Price, U.S. $
Chelex Resins
142-2822 Chelex 100 Resin, 500 g Sodium 50–100 300–1,180 68–76 790.00
142-2832* Chelex 100 Resin, 500 g Sodium 100–200 150–300 68–76 790.00
142-2842** Chelex 100 Resin, 500 g Sodium 200–400 75–150 68–76 950.00
142-2843 Chelex 100 Resin, 10 kg Sodium 200–400 75–150 68–76
1422825 Chelex 100 Resin, 100 g Iron 100–200 150–300 — 790.00
142-2827 Chelex 100 Resin, 1 kg Iron 100–200 150–300 —
142-2826 Chelex 100 Resin, 10 kg Iron 100–200 150–300 —
Catalog # DescriptionPrice, U.S. $
AG Resins in Larger Volumes and Special Packaging for Industrial Applications
140-1256 AG 1-X2 Resin, acetate, 200–400 mesh, 1 kg 790.00
140-1255 AG 1-X2 Resin, acetate, 200–400 mesh, 10 kg 790.00
140-1232 AG 1-X2 Resin, chloride, 50–100 mesh, 10 kg
140-1342 AG 1-X4 Resin, chloride, 100–200 mesh, 10 kg 790.00
140-1352 AG 1-X4 Resin, chloride, 200–400 mesh, 10 kg
140-1447 AG 1-X8 Resin, acetate, 20–50 mesh, 10 kg 790.00
140-1446 AG 1-X8 Resin, acetate, 100–200 mesh, 10 kg 790.00
140-1425 AG 1-X8 Resin, chloride, 20–50 mesh, 1 kg 790.00
140-1457 AG 1-X8 Resin, chloride, 100–200 mesh, 5 kg 790.00
140-1426 AG 1-X8 Resin, chloride, 20–50 mesh, 10 kg 790.00
140-1432 AG 1-X8 Resin, chloride, 50–100 mesh, 10 kg 790.00
140-1456 AG 1-X8 Resin, chloride, 200–400 mesh, 10 kg 790.00
140-1445 AG 1-X8 Resin, chloride, 100–200 mesh, 10 kg 950.00
140-1424 AG 1-X8 Resin, hydroxide, 20–50 mesh, 10 kg 950.00
143-2447 AG 1-X8 Resin, hydroxide, 20–50 mesh, biotechnology grade, 10 kg
143–2448 AG 1-X8 Resin, hydroxide, 100–200 mesh, biotechnology grade, 10 kg
143-2450 AG 1-X8 Resin, hydroxide, 200-400 mesh, biotechnology grade, 10 kg
140-2423 AG 2-X8 Resin, chloride, 20–50 mesh, 10 kg 950.00
140-2341 AG 4-X4 Resin, free base, 100–200 mesh, 5 kg 790.00
142-1232 AG 50W-X2 Resin, hydrogen, 50–100 mesh, 10 kg 790.00
142-1242 AG 50W-X2 Resin, hydrogen, 100–200 mesh, 10 kg 790.00
142-1424 AG 50W-X8 Resin, ultrapure, hydrogen, 20–50 mesh, 10 kg 790.00
142-1423 AG 50W-X8 Resin, hydrogen, 20–50 mesh, 10 kg 950.00
142-1432 AG 50W-X8 Resin, hydrogen, 50–100 mesh, 10 kg 950.00
142-1442 AG 50W-X8 Resin, hydrogen, 100–200 mesh, 10 kg 790.00
142-1452 AG 50W-X8 Resin, hydrogen, 200–400 mesh, 10 kg 790.00
143-1442 AG 50W-X8 Resin, sodium, 100–200 mesh, molecular biology grade, 1 kg
142-1254 AG 50W-X12 Resin, hydrogen, 200–400 mesh, 1 kg 790.00
141-1842 AG MP-1M Resin, chloride, 100–200 mesh, 10 kg 790.00
141-1852 AG MP-1M Resin, chloride, 200–400 mesh, 10 kg 790.00
141-1853 AG MP-1M Resin, nitrate, 200–400 mesh, 10 kg 950.00
142-6427 AG 501-X8 Resin, H+ + OH–, 20–50 mesh, 10 kg 950.00
143-6426** AG 501-X8 Resin, H+ + OH–, 20–50 mesh, 10 kg 950.00
143-7428* AG 501-X8 Resin, H+ + OH–, 20–50 mesh, 10 kg 950.00
143-6427*** AG 501-X8(D) Resin, H+ + OH–, 20–50 mesh, 10 kg
continues

92 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/agresins Analytical Grade Ion Exchange Chromatography

Ordering Information
Catalog# Description Particle Size, µm Ionic Form Application Price, U.S. $
Prepacked Poly-Prep Ion Exchange Columns
7316211 Poly-Prep Columns, AG 1-X8 106–180 Chloride Separation of low molecular 245.00
resin, 100–200 mesh, 50 weight inorganic anions
7316212 Poly-Prep Columns, AG 1-X8 45–106 Chloride For high-resolution general 245.00
resin, 200–400 mesh, 50 purpose separations
7316221 Poly-Prep Columns, AG 1-X8 45–106 Formate Separation of low molecular 245.00
resin, 200–400 mesh, 50 weight biological compounds
such as carboxylic acids
7316213 Poly-Prep Columns, AG 50W-X8 106–250 Hydrogen Separation and concentration 245.00
resin, 100–200 mesh, 50 of low molecular weight cations
such as small peptides and
amino acids
7316214 Poly-Prep Columns, AG 50W-X8 63–150 Hydrogen For high-resolution general 250.00
resin, 200–400 mesh, 50 purpose separations
* Also available as biotechnology grade resin.
** Also available as molecular biology grade resin.
*** Molecular biology grade resin; also available in biotechnology grade.

Technical and Reactor Grade Resins

Bio-Rad offers reactor grade Bio-Rex RG 501-X8 resins for power plant deionization systems and large-scale cleanup
of metals from waste water.
For More Information
Web: bio-rad.com/agresins

Ordering Information
Catalog # Description Ionic Form Dry Mesh Size MW Exclusion Price, U.S. $
Technical and Reactor Grade Resins
444-9998* Bio-Rex RG 501-X8 Resin, 1 ft3, for water purification H+ + OH – 20–50 1,000 Inquire
444-9999 Bio-Rex RG 501-X8 Resin, 500 g H+ + OH – 20–50 1,000 120.00
* 1 ft3 corresponds to approximately 20 kg. Larger volumes and special packaging are available on request.

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 93
 Chromatography Media
Analytical Grade Ion Exchange Chromatography bio-rad.com/agresins

Molecular Biology and Biotechnology Grade Resins

■
Molecular biology grade resins — are chemically ■
B iotechnology grade resins — undergo
identical to the equivalent analytical grade resins and are special processing and contain fewer than
certified to be free of endo- and exonuclease activities 100 microorganisms/gram
and ligase inhibitors For More Information
Web: bio-rad.com/agresins
Request or download bulletins: 6904, 6905, 6906, 6907, and 6908

Ordering Information
Dry Mesh Wet Bead
Catalog # Description Ionic Form Size Size, µm Application Price, U.S. $
Molecular Biology Grade Resins
1436424 AG 501-X8 Resin, H+ + OH– 20–50 300–1,180 Deionization 455.00
molecular biology grade, 100 g
1436425 AG 501-X8(D) Resin, H+ + OH– 20–50 300–1,180 ~1,000 MW limit 470.00
molecular biology grade, 100 g
1421253 Chelex 100 Resin, Sodium 200–400 75–150 DNA extraction for PCR 195.00
molecular biology grade, 50 g sample preparation
Biotechnology Grade Resins
1431255 AG 1-X2 Resin, Hydroxide 200–400 75–180 Separation of small peptides, 755.00
biotechnology grade, 100 g nucleotides, and large metal
complexes
1431345 AG 1-X4 Resin, Hydroxide 100–200 63–150 Separation of organic acids, 445.00
biotechnology grade, 100 g nucleotides, phosphoinositides,
and other anions
1432445 AG 1-X8 Resin, Hydroxide 100–200 106–180 Separation of inorganic and 825.00
biotechnology grade, 100 g organic anions with MW <1,000
1432446 AG 1-X8 Resin, Hydroxide 200–400 45–106 460.00
biotechnology grade, 100 g
1435241 AG 50W-X2 Resin, Hydrogen 100–200 106–300 Separation of peptides, 400.00
biotechnology grade, 100 g nucleotides, and cations
1435341 AG 50W-X4 Resin, Hydrogen 200–400 75–150 Separation of amino acids, 400.00
biotechnology grade, 100 g nucleotides, and cations
1435441 AG 50W-X8 Resin, Hydrogen 100-200 106-250 Separation of amino acids 400.00
biotechnology grade, 100 g and cations
1435451 AG 50W-X8 Resin, Hydrogen 200–400 63–150 400.00
biotechnology grade, 100 g
1437834 AG 11 A8 Resin, Self- 50–100 180–425 Removal of ionic compounds 400.00
biotechnology grade, 100 g adsorbed
1437424 AG 501-X8 Resin, H+ + OH– 20–50 300–1,180 Deionization 400.00
biotechnology grade, 100 g
1437425 AG 501-X8(D) Resin, H+ + OH– 20–50 300–1,180 400.00
biotechnology grade, 100 g
1432832 Chelex 100 Resin, Sodium 100–200 150–300 PCR sample preparation 415.00
biotechnology grade, 100 g
1435832 Bio-Rex 70 Resin, Sodium 50–100 150–300 Separation of cationic proteins 400.00
biotechnology grade, 100 g and amines
1435852 Bio-Rex 70 Resin, Sodium 200–400 45–75 400.00
biotechnology grade, 100 g
1528920 Bio-Beads SM-2, 25 g — — — Detergent removal 125.00
1523920 Bio-Beads SM-2, 100 g — — — Detergent removal 465.00

94 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/mixedmode Mixed-Mode Chromatography

Mixed-Mode Chromatography
Mixed-mode media offer unique separation properties and unparalleled selectivity and resolution for a variety of molecules.
These products can be used at any stage in a process from initial capture to final polishing.

Nuvia Mixed-Mode Resins See Also

Resin sampler packs:
page 120.
Nuvia aPrime 4A anion exchange resin and Nuvia cPrime Nuvia cPrime Hydrophobic Cation Exchange Resin Foresight prepacked
cation exchange resin are part of Bio-Rad’s family of Nuvia cPrime resin’s unique selectivity allows method plates and columns:
pages 86, 130, 177.
mixed-mode purification products. These resins are developers to use hydrophobic and cation exchange
designed for process-scale purification of a wide variety interaction modes to achieve effective purification.
of therapeutic proteins. Importantly, the resin has a large design space for binding
and elution, allowing for the development of highly robust
Nuvia mixed-mode resins deliver value by providing: methods in a commercial manufacturing setting. Nuvia
■
Unique selectivity cPrime is built on a rigid, mechanically and chemically
■
Simple method development stable macroporous base matrix with a particle size
optimized to deliver exceptional flow properties, fast mass
■
 arge design space for capture and elution of a variety
L
transfer, and stability.
of biotherapeutic proteins
For More Information
■
High recovery of target protein
Web: bio-rad.com/nuvia
■
Mechanical and chemical stability bio-rad.com/aprime
Request or download bulletins: 6241, 6242, and 6418

Nuvia aPrime 4A Hydrophobic Anion Exchange Resin

Nuvia aPrime 4A resin is designed to provide optimal
efficiency and productivity for process scale purification
of therapeutic proteins. The resin's ligand density and
hydrophobicity are engineered to facilitate selective and
reversible binding of target molecules for high purity and
yield. Nuvia aPrime 4A resin can be used for both capture
and polish purification. It offers a wide design space and
straightforward method development to purify even the
most complex molecules.

Properties of Nuvia aPrime 4A and Nuvia cPrime

Property Nuvia aPrime 4A Nuvia cPrime

Functional group Aromatic hydrophobic anion exchanger Aromatic hyrdophobic cation exchanger
Median particle size 50 µm 70 µm
Dynamic binding capacity ≥50 mg/ml at 300 cm/hr* hlgG: >40 mg/ml (at 10% breakthrough, 300 cm/hr)
Ligand density 100 ± 20 µeq/ml 123 ± 20 µeq/ml
Recommended linear flow rate range 50–300 cm/hr 50–600 cm/hr
pH stability** Short term: 2–14 1–14 short term
3–13 long term
Chemical stability† 1 N NaOH, 1 M HCl, 25% acetic acid, 8 M urea, 25% acetic acid, 8 M urea, 6 M guanidine-HCI,
6 M guanidine HCl, 3 M NaCl, 1% Triton X-100, 1 N NaOH, 1 M HCI, 6 M potassium thiocyanate,
20–70% ethanol, 30% isopropanol 3 M sodium chloride, 1% Triton X-100, 2% SDS,
20–70% ethanol, 30% isopropyl alcohol
Shipping solution 20% ethanol + 1 M NaCl 20% ethanol, 30 mM Na2SO3
Storage conditions 20% ethanol 0.1 N NaOH
* 10% breakthrough capacity determined with 1.0 mg/ml of an acidic monoclonal antibody (mAb; pI ~6.9) in 20 mM NaPO4, pH 7.8.
** No significant change in ligand density after 200 hr contact at 22°C.
† No significant change in ligand density after 1 week contact at 22°C.

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 Chromatography Media
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Ordering Information
Catalog # Description
Nuvia cPrime Media
1563401 Nuvia cPrime Media, 25 ml 141.84
1563402 Nuvia cPrime Media, 100 ml 471.76
156-3403 Nuvia cPrime Media, 500 ml Inquire
156-3404 Nuvia cPrime Media, 1 L Inquire
156-3405 Nuvia cPrime Media, 5 L Inquire
156-3406 Nuvia cPrime Media, 10 L Inquire
Nuvia cPrime Foresight Plates, Columns, and RoboColumn Units
732-4705* Foresight Nuvia cPrime Plates, 20 µl 551.25
732-4722 Foresight Nuvia cPrime Column, 1 ml 141.95
732-4742 Foresight Nuvia cPrime Column, 5 ml 278.22
732-4807** Foresight Nuvia cPrime RoboColumn Unit, 200 µl 231.53
732-4808** Foresight Nuvia cPrime RoboColumn Unit, 600 µl 277.83
Nuvia aPrime 4A Media
12007397 Nuvia aPrime 4A Media, 25 ml
12007396 Nuvia aPrime 4A Media, 100 ml
12007379 Nuvia aPrime 4A Media, 500 ml
12007380 Nuvia aPrime 4A Media, 5 L
12007391 Nuvia aPrime 4A Media, 10 L
Foresight Nuvia aPrime 4A Plates, Columns, and RoboColumn Units
12007411* Foresight Nuvia aPrime 4A Plates, 20 µl
12007392 Foresight Nuvia aPrime 4A Column, 1 ml
12007393 Foresight Nuvia aPrime 4A Column, 5 ml
12007394** Foresight Nuvia aPrime 4A RoboColumn, 200 µl
12007395** Foresight Nuvia aPrime 4A RoboColumn, 600 µl
EconoFit Columns
12009281 EconoFit Nuvia cPrime Column, 1 x 1 ml, 7 x 25 mm, maximum pressure 45 psi
12009280 EconoFit Nuvia aPrime 4A Column, 1 x 1 ml, 7 x 25 mm, maximum pressure 45 psi
EconoFit Prepacked Column Kits
12009315 EconoFit Aggregate Clearance Kit, includes one 1 ml each of Nuvia cPrime, Nuvia aPrime 4A,
Nuvia HR-S, CHT XT
12009314 DISCONTINUED
EconoFit Large Biomolecule Kit, includes 1 ml each of Nuvia Q; Nuvia HP-Q; Nuvia cPrime;
Nuvia aPrime 4A; CHT II, 40 µm; and CHT XT
12009313 EconoFit mAb Kit, includes 1 ml each of Nuvia S, Nuvia Q, Nuvia cPrime, Nuvia aPrime 4A, Nuvia HR-S,
and CHT XT
* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.

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Hydroxyapatite (Ca 5(PO4)3OH) 2 and fluoroapatite Ca10(PO4) 6F2 are forms of calcium phosphate that offer unique selectivities
and often separate biomolecules that appear homogeneous when other chromatographic and electrophoretic techniques
are used. Hydroxyapatite and fluoroapatite chromatography can be used at any stage from initial capture to final polishing.

Ceramic Hydroxyapatites See Also

Foresight prepacked
plates and columns:
CHT ceramic hydroxyapatite is a spherical, macroporous, offering flexibility to select the type that provides maximum pages 86, 130, 177.
and chemically pure form of hydroxyapatite that has been benefit in a given separation. CHT Type I and XT, with their
sintered at high temperatures to yield a physically and smaller pore diameter and larger surface area, have higher
chemically robust support. It gives consistent, reproducible binding capacities than CHT Type II. CHT Type II with its
results over many cycles at scales up to 1.8 m, making larger pore diameter provides higher capacity for large
CHT ceramic hydroxyapatite ideal for large-scale molecules, such as IgM and virus particles.
bioprocess applications. It has unique separation ■
High throughput
properties and unmatched selectivity and resolution.
Applications include the purification of enzymes, antibody ■
High physical and chemical stability
fragments, bispecific antibodies, and monocolonal and ■
Unmatched selectivity
polyclonal antibodies of various classes; separation of ■
Single-step clearance of impurities and aggregates
supercoiled DNA from linear duplexes as well as single-
stranded from double-stranded DNA; and efficient isolation
■
Rapid and simple column packing
and purification of viruses and virus-like particles. CHT For More Information
ceramic hydroxyapatite is available in three distinct versions: Web: bio-rad.com/cht
Type I, XT, and Type II. The three types differ in their pore bio-rad.com/chtxt
Request or download bulletins: 5667, 5709, 5853, 6086, 6549,
diameters and can differ slightly in protein separation, and 7080

Specifications
CHT Type I CHT Type II CHT XT
Functional groups Ca 2+, PO43–, OH – Ca 2+, PO43–, OH – Ca 2+, PO43–, OH –
Observed dynamic binding 25–40 mg lysozyme/g* 12.5–20 g lysozyme/g* 17.4–24.8 mg lysozyme/g*
capacity, lysozyme
Median particle size, µm 20, 40, 80 20, 40, 80 40
Tap settled density* 0.63 g/ml 0.63 g/ml 0.67 g/ml
(g/ml tap settled bed)
* Under ideal conditions.

Properties of CHT
Property CHT Type I CHT Type II CHT XT
Observed dynamic binding 25–60 mg IgG/ml 15–25 mg IgG/ml >60 mg/ml mAb G** (pI 9.2)
capacity, IgG*
Typical linear flow rate 50–400 cm/hr 50–400 cm/hr 50–400 cm/hr
pH stability 6.5–14 6.5–14 6.5–14
Regeneration 0.4 M sodium phosphate 0.4 M sodium phosphate 0.4 M sodium phosphate, pH
pH 7; 1 M trisodium phosphate, pH 7; 1 M trisodium phosphate, 7.0–7.5; 1 M trisodium phosphate,
pH 11–12 pH 11–12 pH 11–12
Autoclavability (bulk) 121°C, 20 min in phosphate 121°C, 20 min in phosphate 121°C, 20 min in phosphate
buffer, pH 7 buffer, pH 7 buffered saline, pH 7
Sanitization 1–2 N NaOH 1–2 N NaOH 1–2 N NaOH
Recommended column storage 0.1 N NaOH 0.1 N NaOH 0.1 N NaOH
* At 500 cm/hr with 10 mM sodium phosphate, pH 6.8.
** At 100 cm/hr with 5 mM sodium phosphate, 25 mM sodium chloride, pH 7 in a 0.5 x 9.5 cm column

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Ordering Information
Description Type I Type II XT
CHT Ceramic Hydroxyapatite, 20 µm
20 µm particle size, 10 g 1582000 1582200 —
20 µm particle size, 100 g 1570020 1572000 —
20 µm particle size, 1 kg (1.6 L) 157-0021 157-2100 —
CHT Ceramic Hydroxyapatite, 40 µm
40 µm particle size, 10 g 1584000 1584200 12002457
40 µm particle size, 100 g 1570040 1574000 12002454
40 µm particle size, 1 kg (1.6 L) 157-0041 157-4100 12002456
40 µm particle size, 5 kg (7.9 L) 157-0045 157-4500 12002455
CHT Ceramic Hydroxyapatite, 80 µm
80 µm particle size, 10 g 1588000 1588200 —
80 µm particle size, 100 g 1570080 1578000 —
80 µm particle size, 1 kg (1.6 L) 157-0081 157-8100 —
80 µm particle size, 5 kg (7.9 L) 157-0085 157-8500 —
Foresight CHT Plates, Columns, and RoboColumn Units
Foresight CHT Plates, 40 µm, 20 µl* 732-4716 732-4718 12003151
Foresight CHT Column, 40 µm, 1 ml 732-4735 732-4736 12003150
Foresight CHT Column, 40 µm, 5 ml 732-4755 732-4756 12003149
Foresight CHT RoboColumn Unit, 40 µm, 200 µl** 732-4822 732-4825 12003152
Foresight CHT RoboColumn Unit, 40 µm, 600 µl** 732-4823 732-4826 12003148
Catalog # Description
EconoFit CHT Columns***
12009255 EconoFit CHT Type I, 40 µm Column, 1 x 1 ml, 7 x 25 mm
12009253 EconoFit CHT Type I, 40 µm Column, 1 x 5 ml, 16 x 25 mm
12009254 EconoFit CHT Type I, 40 µm Columns, 5 x 5 ml, 16 x 25 mm
12009256 EconoFit CHT Type I, 80 µm Column, 1 x 1 ml, 7 x 25 mm
12009259 EconoFit CHT Type II, 40 µm Column, 1 x 1 ml, 7 x 25 mm
12009257 EconoFit CHT Type II, 40 µm Column, 1 x 5 ml, 16 x 25 mm
12009258 EconoFit CHT Type II, 40 µm Columns, 5 x 5 ml, 16 x 25 mm
12009260 EconoFit CHT Type II, 80 µm Column, 1 x 1 ml, 7 x 25 mm
12009261 EconoFit CHT XT Column, 1 x 1 ml, 7 x 25 mm
Description 1 x 5 ml 5 x 5 ml
CHT Prepacked Bio-Scale Mini Cartridges
CHT Type I Ceramic Hydroxyapatite 7324322 7324324
CHT Type II Ceramic Hydroxyapatite 7324332 7324334

Catalog # Description
Bio-Scale Purification Kits
7324407 Bio-Scale Mini Apatite Purification Kit, CHT Type II cartridge, CFT Type II cartridge, 1 x 5 ml each
7324408 DISCONTINUED
 io-Scale Mini mAb Purification Kit, UNOsphere
B SUPrA affinity cartridge, UNOsphere Q cartridge, CHT Type I cartridge,
1 x 5 ml each
EconoFit Application Kits
12009315 EconoFit Aggregate Clearance Kit, includes 1 ml each of Nuvia cPrime, Nuvia aPrime 4A, Nuvia HR-S, CHT XT
12009309 EconoFit CHT Kit, includes 1 ml each of CHT I, 40 µm; CHT II, 40 µm; MPC I, 40 µm; and CFT II, 40 µm
12009314 EconoFit Large Biomolecule Kit, includes 1 ml each of Nuvia Q; Nuvia HP-Q; Nuvia cPrime; Nuvia aPrime 4A;
CHT II, 40 µm; and CHT XT
12009313 EconoFit mAb Kit, includes 1 ml each of Nuvia S, Nuvia Q, Nuvia cPrime, Nuvia aPrime 4A, Nuvia HR-S, and CHT XT

Larger volumes and special packaging for industrial applications are available on request.
* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.
*** Maximum pressure 72 psi.

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Ceramic Fluoroapatites

MPC Ceramic Hydroxyfluoroapatite CFT Ceramic Fluoroapatite

MPC ceramic hydroxyfluoroapatite Ca10(PO4)6(OH)1.5(F)0.5 CFT ceramic fluoroapatite (Ca10(PO4)6F2) is a rigid spherical
is a complementary addition to our line of ceramic apatite macroporous media used in the purification of biologically
mixed-mode chromatography media. MPC is a composite significant compounds. CFT is a composite of fluoroapatite
of hydroxyapatite and fluoroapatite, which confers greater and hydroxyapatite prepared by chemically converting
pH stability. MPC has the unique separation properties and hydroxyapatite nanocrystals to fluoroapatite with a fluorine
unmatched selectivity and resolution of CHT. reagent. CFT can be used under stringent chromatography
requirements to separate acidic proteins requiring buffered
conditions as low as pH 5.6.
Specifications
MPC MPC CFT
Functional groups Ca2+, PO43–, OH –, F– Ca 2+, PO43–, F–
Observed dynamic binding ≥25 mg lysozyme/g 14–21.5 mg lysozyme/g
capacity lysozyme
Median particle size, µm 40 40
Tap settled density* 0.72 g/ml 0.86 g/ml
(g/ml tap settled bed)
* Under ideal conditions.

Properties of MPC and CFT

Property MPC CFT*
Observed dynamic binding capacity lgG 25–50 mg lgG/ml** 33 mg lgG/ml
Typical linear flow rate range 50–400 cm/hr 50–400 cm/hr
pH stability 6.5–14 5–14
Autoclavability (bulk) 121ºC, 20 min in phosphate buffer, pH 7 121°C, 20 min in phosphate buffer, pH 7
Sanitization 1–2 N NaOH 1–2 N NaOH
Recommended column storage 0.1 N NaOH 0.1 N NaOH
* A small amount (up to 5 mM) of sodium phosphate should be added to all unbuffered solutions as a counterion.
** 4
 0 μm particles, 300 cm/hr, 5 mM sodium phosphate, 25 mM NaCl, pH 6.5.

For More Information

Web: bio-rad.com/mpc
Web: bio-rad.com/cft
Request or download bulletins: 6432 and 6086 (MPC); 3111 and 5853 (CFT)

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 Chromatography Media
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Ordering Information
Catalog # Description Price, U.S. $
MPC Ceramic Hydroxyfluoroapatite, 40 µm
1580200 40 µm particle size, 10 g
1570200 40 µm particle size, 100 g
157-0201 40 µm particle size, 1 kg (1.4 L)
157-0205 40 µm particle size, 5 kg (6.9 L)
MPC Foresight Plates, Columns, and RoboColumn Units
732-4785 Foresight MPC Type I Plates, 20 μl*
732-4737 Foresight MPC Type I Column, 40 µm, 1 ml
732-4757 Foresight MPC Type I Column, 40 µm, 5 ml
732-4828 Foresight MPC Type I RoboColumn Unit, 40 µm, 200 µl**
732-4829 Foresight MPC Type I RoboColumn Unit, 40 µm, 600 µl**
CFT Ceramic Fluoroapatite
1585200 40 µm particle size, 10 g 585.00
1575000 40 µm particle size, 100 g 1,660.00
157-5100 40 µm particle size, 1 kg (1.2 L) Inquire
157-5500 40 µm particle size, 5 kg (5.8 L) Inquire

EconoFit Columns***
12009279 EconoFit MPC I-40 Column, 1 x 1 ml, 7 x 25 mm
12009252 EconoFit CFT Type II, 40 µm Column, 1 x 1 ml, 7 x 25 mm
12009240 EconoFit CFT Type II, 40 µm Column, 1 x 5 ml, 16 x 25 mm
12009251 EconoFit CFT Type II, 40 µm Columns, 5 x 5 ml, 7 x 25 mm
Price, U.S. $
Description 1 x 5 ml Price, U.S. $ 5 x 5 ml
CFT Prepacked Bio-Scale Mini Cartridges
CFT Type II Ceramic Fluoroapatite 7324405 155.00 7324406 615.00

Catalog # Description Price, U.S. $

DISCONTINUED
Application Kits
7324407 Bio-Scale Mini Apatite Purification Kit, CHT Type II cartridge, CFT Type II cartridge, 1 x 5 ml each 585.00
7324408 Bio-Scale Mini mAb Purification Kit, UNOsphere SUPrA affinity cartridge, UNOsphere Q cartridge, CHT
Type I cartridge, 1 x 5 ml each
12009309 EconoFit CHT Kit, includes 1 ml each of CHT I, 40 µm; CHT II, 40 µm; MPC I, 40 µm; and CFT II, 40 µm 1,660.00

* Package size: 2 x 96-well plates.

** Package size: 1 row of 8 columns.
*** Maximum pressure 72 psi.

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 Chromatography Media
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Bio-Gel Hydroxyapatite HT and HTP See Also

CHT ceramic
hydroxyapatite:
Hydroxyapatite (Ca 5(PO4 )3OH)2 is a form of calcium phosphate page 97.
used in the chromatographic separation of biomolecules. Bio-Scale Mini CHT
cartridges: page 132.
Bio-Gel crystalline hydroxyapatite media is compatible with
Bio-Scale CHT Type I
a wide range of aqueous buffers and organic modifiers and columns: page 136.
can be sanitized in up to 1 M NaOH. Typical pH tolerance is
>6.8, however it can be used at 5.5 in single-use applications.
Bio-Gel hydroxyapatite can be autoclaved in buffers that
maintain a pH above 7 during the autoclaving cycle.
■
 io-Gel HT media — shipped in 10 mM sodium
B Bio-Gel HT and HTP
phosphate, pH 6.8 buffer containing 0.02% NaN3. The
flow rate range is 25–100 cm/hr at 10 cm bed height in Properties of Bio-Gel HT and HTP
a gravity-packed column. Bio-Gel HT media has a shelf
Bio-Gel Bio-Gel Bio-Gel HTP
life of at least one year when stored at 4°C in the shipping Property HT HTP (DNA Grade)
buffer
Flow rate,* cm/hr >25 >25 >5
■
 io-Gel HTP powder — the dry form of Bio-Gel HT media.
B BSA adsorbed,** 10 10 10
When hydrated, it should be stored similarly to Bio-Gel HT mg per dry gram
media; the flow rate range is similar to Bio-Gel HT media Calf thymus DNA >500 >500 >800
■
 NA grade Bio-Gel HTP powder — a smaller particle
D adsorbed, μg per
dry gram
size version of Bio-Gel HTP powder, it exhibits higher
capacity for biomolecules. It is generally used for Hydrated volume — 2–3 ml/g 2–3 ml/g
single- and double-stranded DNA separations. * Flow rate determined on a 1.5 x 10 cm column with 40 cm
It has an increased capacity for RNA, making it useful hydrostatic pressure.
** Batch-wise uptake.
for DNA-RNA hybridization studies. Its flow rate is limited
in gravity feed columns, but it can be used in medium- For More Information
pressure columns to enhance flow rate Web: bio-rad.com/biogelHTandHTP
Download bulletin: LIT217

Ordering Information
Catalog # Description Price, U.S. $
1300150 Bio-Gel HT Hydroxyapatite, hydrated, 250 ml 315.00
130-0151 Bio-Gel HT Hydroxyapatite, hydrated, 500 ml 545.00
1300420 Bio-Gel HTP Hydroxyapatite, powder, 100 g 345.00
130-0421 Bio-Gel HTP Hydroxyapatite, powder, 1 kg Inquire
130-0425 Bio-Gel HTP Hydroxyapatite, powder, 5 kg Inquire
1300520 Bio-Gel HTP Hydroxyapatite, DNA grade, 100 g 620.00

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 Chromatography Media
Affinity Chromatography bio-rad.com/affinityresin

Affinity Chromatography

Nuvia IMAC Resin

Nuvia IMAC resin is a high-capacity, next-generation metal

Dynamic binding capacity, 10% BT

60
chelate affinity chromatography resin. Nuvia IMAC delivers
50
high binding capacity over a range of pH and flow rates,
providing wide experimental design space for many 40

applications. Immobilized metal affinity chromatography 30

(IMAC) is a powerful purification technique that relies on the 20
affinity that the histidine tag has for immobilized transition
10
metals. Nuvia IMAC resins contain nitrilotriacetic acid (NTA)
as the chelating ligand for di- or trivalent metal ions. Its 0
50 100 150 200 250 300 350
chemical structure allows highly selective binding of
Linear velocity, cm/hr
recombinant histidine-tagged proteins when charged with
Ni2+ or other transition metals, such as Zn2+ or Cu2+. The Comparison of dynamic binding capacity vs. flow velocity of
Nuvia IMAC resin ( ), agarose IMAC resin A (n), and agarose IMAC
optimized particle size and high porosity allow operation at
resin B ( ). BT, breakthrough.
increasingly higher flow rates without compromising binding
capacity, resulting in superior mass transfer characteristics. Column pressure (net), psi 50

Column pressure (net), bar

3
Nuvia IMAC is available in two forms: uncharged and charged 40
with Ni2+. The uncharged form can be charged with the metal 30 2
ion of your choice for even greater purification flexibility. 20
Nuvia IMAC can be used at any scale from laboratory to 10
1
manufacturing, providing robust, reproducible, and efficient
0 0
purifications each time. Nuvia IMAC resin delivers value and 75 125 175 225 275 325 375 425
flexibility by providing users: Linear velocity, cm/hr

■
 imple scale-up from laboratory to bioprocess
S Flow performance of Nuvia IMAC resin. 20 x 20 cm column,
manufacturing scale 1.2 compression factor.
■
Optimized particle size for high flow rate and ■
Excellent recovery and reproducibility
low backpressure ■
Full regulatory support
■
Superior dynamic binding capacity at high flow rates
■
Robust base matrix withstands repeated clean-in-place/ For More Information
Web: bio-rad.com/nuviaIMACresin
recharging cycles Download bulletin: 6859

Ordering Information
Catalog # Description Properties (all listed products) Price, U.S. $
Nuvia IMAC Resins
7800800 Nuvia IMAC Ni-Charged Resin, 25 ml • Ultra-high capacity at high flow rates 300.00
7800801 Nuvia IMAC Ni-Charged Resin, 100 ml • pH stability across broad range 1,010.00
780-0802 Nuvia IMAC Ni-Charged Resin, 500 ml • Robust beads 1,010.00
12003233 Nuvia IMAC Ni-Charged Resin, 5 L 1,010.00
12002782 Nuvia IMAC Ni-Charged Resin, 10 L 1,010.00
12004040 Nuvia IMAC Uncharged Resin, 5 L
12004039 Nuvia IMAC Uncharged Resin, 10 L
Nuvia IMAC Foresight Plates, Columns, and RoboColumn Units
12004035* Foresight Nuvia IMAC Ni-Charged Plates, 20 µl 1,010.00
12004038 Foresight Nuvia IMAC Ni-Charged Column, 1 ml 1,010.00
12004037 Foresight Nuvia IMAC Ni-Charged Column, 5 ml 1,010.00
12004051** Foresight Nuvia IMAC Ni-Charged RoboColumn 1,010.00
Unit, 200 µl
12004052** Foresight Nuvia IMAC Ni-Charged RoboColumn Inquire
Unit, 600 µl
* Package size: 2 x 96-well plates. ** Package size: 1 row of 8 columns. continues

102 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
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See Also
Ordering Information
Affinity media
Catalog # Description Price, U.S. $ selection guide:
page 82.
Bio-Scale Mini Nuvia IMAC Cartridges
Bio-Scale Mini
7800811 Bio-Scale Mini Nuvia IMAC Cartridge, 5DISCONTINUED
ml 300.00 cartridges:
7800812 Bio-Scale Mini Nuvia IMAC Cartridges, 5 x 5 ml 1,010.00 page 132.
EconoFit Nuvia IMAC Columns and Application Kit DISCONTINUED IMAC purification
kits: page 108.
12009288 EconoFit Nuvia IMAC Column, 1 x 1 ml, 7 x 25 mm
12009286 EconoFit Nuvia IMAC Column, 1 x 5 ml, 16 x 25 mm
12009285 EconoFit Nuvia IMAC Columns, 5 x 1 ml, 1 ml, 7 x 25 mm
12009287 EconoFit Nuvia IMAC Columns, 5 x 5 ml, 16 x 25 mm
12009289 EconoFit Nuvia IMAC Uncharged Column, 1 x 1 ml, 7 x 25 mm
12009317 EconoFit Desalting Kit, includes 2 x 5 ml each of Bio-Gel P-6 and Nuvia IMAC
DISCONTINUED

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 103
Discontinued products are no longer available.
 Chromatography Media
Affinity Chromatography bio-rad.com/affinityresin

Profinity IMAC Resins and Cartridges

Profinity IMAC resins are affinity chromatography

supports for the purification of recombinant histidine-
tagged proteins. Profinity IMAC resins, based on
UNOsphere beads, contain iminodiacetic acid (IDA)
as the chelating ligand for di- or trivalent metal ions.
Its chemical structure allows highly selective binding of
recombinant histidine-tagged proteins when charged
with Ni2+ or other transition metals such as Zn2+ or Cu2+.
They offer high capacity at high flow rates and can be
used under either nondenaturing or denaturing conditions.
The resins are suitable for purification using liquid Profinity IMAC Resins
chromatographic instrumentation, gravity-flow columns,
or spin columns. The resin is available in two forms:
uncharged and precharged with Ni 2+. The uncharged
form can be charged with the metal ion of your choice
for even greater purification flexibility.
Features of Profinity IMAC resins include:
■
Optimal ligand density for higher purity of target protein
■
Superb mechanical strength
■
 xcellent pressure-flow properties for high maximum
E
operating pressures and flow rates, allowing rapid Bio-Scale Mini Profinity IMAC Cartridges
purification, column cleaning, and re-equilibration
■
Stability from pH 1–14
■
 ompatibility with denaturing agents, detergents,
C
and reducing agents
For More Information
Web: bio-rad.com/profinityIMAC

Ordering Information
Catalog # Description Properties (all listed products) Price, U.S. $
Ready-to-Use Affinity Resins
1560121 Profinity IMAC Uncharged Resin, 10 ml • Stable from pH 1 to 14 81.00
1560123 Profinity IMAC Uncharged Resin, 50 ml • Strong bead matrix for gravity flow 350.00
1560131 Profinity IMAC Ni-Charged Resin, 10 ml 100.00
1560133 Profinity IMAC Ni-Charged Resin, 25 ml 245.00
1560135 Profinity IMAC Ni-Charged Resin, 100 ml 905.00
156-0137 Profinity IMAC Ni-Charged Resin, 500 ml Inquire

Description 5 x 1 ml Price, U.S. $ 1 x 5 ml Price, U.S. $ 5 x 5 ml Price, U.S. $

Prepacked Bio-Scale Mini Cartridges
Profinity IMAC Ni-Charged Resin DISCONTINUED
7324610 225.00 7324612 155.00 7324614 540.00
Larger volumes and special packaging for industrial applications are available on request.

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 Chromatography Media
bio-rad.com/affinityresin Affinity Chromatography

Profinity Epoxide Resin

Profinity epoxide resin is an activated affinity

chromatography support for the immobilization of
biomolecules. Profinity epoxide is a useful support for the
immobilization of ligands that contain nucleophiles such
as amino, thiol, or hydroxyl groups. These groups couple
to the epoxy groups on the resin, which is then used for the
purification of proteins, carbohydrates, or DNA.
Profinity epoxide resin is based on UNOsphere beads,
which have an open pore structure. The open pore
structure allows coupling of large ligands for the purification
of large targets such as Protein A, recombinant proteins
containing MBP, and calmodulin. It is supplied as a dry
powder (1 g of powder gives ~8 ml final volume).
For More Information
Profinity Epoxide Resins
Web: bio-rad.com/profinityepoxide

Ordering Information
Catalog # Description Price, U.S. $
1560200 Profinity Epoxide Resin, 5 g 120.00
1560201 Profinity Epoxide Resin, 25 g 575.00

Profinity GST Cartridges and Kits

Profinity GST resin is an affinity chromatography support

for the purification of recombinant GST-tagged proteins.
Bio-Scale Mini Profinity GST cartridges are 1 and 5 ml
Bio-Scale Mini cartridges filled with Profinity GST resin.
For More Information
Web: bio-rad.com/profinityGST

Ordering Information
Catalog # Description Price, U.S. $
7324620 Bio-Scale Mini Profinity GST Cartridges, 5 x 1 ml 405.00
7324622 Bio-Scale Mini Profinity GST Cartridge, 1 x 5 ml 390.00
7324624 Bio-Scale Mini Profinity GST Cartridges, 5 x 5 ml 1,370.00
6200240
DISCONTINUED
GST Buffer Kit, includes GST lysis, wash, and elution buffers 155.00
6200243 GST Purification Kit, 1 ml, includes GST purification buffer kit and 2 x 1 ml GST cartridges 330.00
6200244 GST Purification Kit, 5 ml, includes 2 GST purification buffer kits and 1 x 5 ml GST cartridge 515.00
12009296 EconoFit Profinity GST Column, 1 x 5 ml
12009295 EconoFit Profinity GST Columns, 5 x 1 ml
12009297 EconoFit Profinity GST Columns, 5 x 5 ml

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Discontinued products are no longer available.
 Chromatography Media
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See Also Profinity eXact Resins and Cartridges

Bio-Scale Mini
cartridges:
page 132. Profinity eXact Purification Resin, Prepacked
Cartridges, and Mini Spin Columns
The Profinity eXact purification resin consists of a highly
engineered subtilisin protease conjugated to an agarose-
based matrix. Purity of the eluted protein using this
resin is typically higher than that for other affinity-tag
systems due to the specific recognition of subtilisin
for its prodomain sequence (KD <100 pM).
The resin can be packed into different column formats,
including low- to medium-pressure columns, gravity-
flow columns, and mini spin columns, offering added
purification flexibility. Bio-Scale Mini cartridges are
available in 1 and 5 ml volumes. Mini spin columns contain
0.1 ml resin. Additional advantages of Profinity eXact
Profinity eXact Resin Cartridges and Bottles
purification resin include:
■ Purificationand processing of fusion-tagged proteins
in a single step Profinity eXact pPAL7 Supercoiled Expression
■
On-column cleavage in as little as 30 min Vector Kit
The pPAL7 supercoiled plasmid expression vector is
■
No protease addition is required used for routine cloning of target DNA sequences using
■ Precisecleavage at N-terminus to generate native a conventional restriction digest cloning strategy.
protein sequence
Profinity eXact Antibody Reagent
Resin is supplied in 100 mM sodium phosphate (pH 7.2) The Profinity eXact antibody reagent is a mouse
containing 0.02% sodium azide. It is also available prepacked monoclonal antibody used to detect expression of the
in 1 and 5 ml cartridges and in mini spin columns. target protein. The Profinity eXact antibody specifically
recognizes the prodomain of the subtilisin protease,
Profinity eXact Purification Starter Kit which is fused to the protein of interest. For convenient
The Profinity eXact expression and purification starter kit western blot detection of the fusion protein, Bio-Rad
can be used to easily evaluate Profinity eXact fusion-tag offers colorimetric detection kits.
technology. It is suitable for new purifications requiring tag
removal and for purifications where cleavage has resulted For More Information
Web: bio-rad.com/affinitycartridges
in inferior results. Download bulletin: 5655
■ Profinity eXact mini spin purification starter kit —
for single-step purification and on-column cleavage of
the tagged protein; also includes a lyophilized lysate to
ensure that preliminary purifications are a success
Resin Specifications
Functional ligand Subtilisin protease, 27.8 kD
Base bead 6% agarose bead
Form 50% suspension in 100 mM sodium phosphate (pH 7.2), 0.02% sodium azide
Particle size range 60–160 µm
Dynamic binding capacity* >3 mg tag-free protein/ml resin
Recommended linear flow rate 1,000 cm/hr at 25°C
pH stability 2–13
Chemical compatibility Common reagents, including detergents, reducing agents, buffering agents, and additives
Storage 4°C
Shelf life in 20% ethanol >1 year at 4°C
Operational temperature 4–40°C
* Dynamic binding capacity determination of a 40 kD maltose binding protein.
Note: Dynamic binding capacity is protein dependent.

106 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/affinityreagents Affinity Chromatography

Ordering Information
Catalog # Description Price, U.S. $
1563007 Profinity eXact Mini Spin Columns, includes 10 spin columns, ten 2 ml capped tubes, 165.00
and ten 2 ml capless tubes
7324646 Bio-Scale Mini Profinity eXact Cartridges, 2 x 1 ml 215.00
7324647 Bio-Scale Mini Profinity eXact Cartridges, DISCONTINUED
4 x 1 ml 370.00
7324648 Bio-Scale Mini Profinity eXact Cartridge, 1 x 5 ml 400.00
1563002 Profinity eXact pPAL Supercoiled Expression Vector Kit, includes 100 µl of 100 ng/µl vector, 20 reactions 215.00
1563004 Profinity eXact Monoclonal Antibody, 100 DISCONTINUED
µl, 1 mg/ml 305.00
1563005 Profinity eXact Purification Resin,* 10 ml 555.00
1563006 DISCONTINUED
Profinity eXact Mini Spin Purification Starter Kit, includes 10 prepacked spin columns, 50 x 2 ml collection 485.00
tubes, lyophilized control protein lysate, bacterial lysis reagent, 50 ml bind/wash buffer, 20 ml elution buffer
12009292 EconoFit Profinity eXact Column, 1 x 1 ml
12009294 EconoFit Profinity eXact Column, 1 x 5 ml
12009293 EconoFit Profinity eXact Columns, 5 x 1 ml
* Larger volumes are available on request.

Profinia System Reagents and Kits

Buffers Reagents
Bio-Rad premade affinity buffer kits for recombinant- Individually packaged reagents are available for use with
tagged protein purification come with concentrated formula the Profinia system. While designed specifically to work
sufficient for ten purifications for a 1 ml affinity cartridge. with the Profinia system, these reagents also have general
These kits provide easy and fast purification for histidine- applicability within all stages of an affinity purification
tagged (native IMAC buffer) proteins. workflow, from sample preparation to protein detection.
■
 enaturing IMAC purification can be performed with
D Histidine and GST Purification E. coli Control Lysate
native buffer IMAC kits with the addition of urea in the The histidine and GST purification E. coli control lysate is a
purification buffers lyophilized, dual-tagged 51 kD target protein that is meant
■
 its for desalting and cartridge cleaning after affinity
K to eliminate concerns over variability in purification buffer
purification are also available solutions or in the purification matrix itself. The control
lysate facilitates system setup and initial purification;
■
 urification kits containing both buffer kits and
P
it is included in every Profinia IMAC and GST starter kit.
appropriate cartridges are provided, if purification
cartridges are desired Glutathione Reagent
For More Information
Powdered glutathione reagents are available for use with GST
Web: bio-rad.com/profiniareagents applications. The glutathione pack is essential for eluting
Download bulletins: 3193, 5283, 5444, and 5456 GST fusion proteins from immobilized glutathione resins.

Kits Histidine and GST Antibodies

Buffer and starter kits can be used with any instrument, Histidine-tagged and GST-tagged monoclonal antibodies
gravity-flow, or spin-column purification procedure. are used to detect target protein expression of overexpressed
6x histidine and GST fusion proteins. They are supplied at
Profinia purification, buffer, and starter kits are designed a concentration of 1 mg/ml in phosphate buffered saline
specifically for use on the Profinia protein purification (pH 7.4) with 0.05% NaN3.
system for time savings and highly reproducible results.
These kits are installed directly on the system; manual
dilution of the concentrated buffers provided in the kits is
not required.
For More Information
Web: bio-rad.com/profiniareagents
Download bulletin: 5574

Histidine and GST Glutathione Histidine and GST Antibodies

Purification E. coli
Control Lysate

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 107
Discontinued products are no longer available.
 Chromatography Media
Affinity Chromatography bio-rad.com/affinityreagents

Affinity Purification Kit Selection Guide

Buffer Sets Bio-Scale Mini Cartridges*
Kit Lysis, Wash, and Elution Desalting and Cartridge Cleaning Affinity Desalting
IMAC Kits
Native IMAC buffer kit •
Native IMAC purification kits (1 and 5 ml) • •
Profinia native IMAC buffer kit • •
Profinia native IMAC purification kits (1 and 5 ml) • • • •
Profinia native IMAC starter kit • • • •
GST Kits
GST buffer kit •
GST purification kits (1 and 5 ml) • •
Profinia GST buffer kit • •
Profinia GST purification kits (1 and 5 ml) • • • •
Profinia GST starter kit • • • •
Desalting and Cartridge Cleaning Kits
Desalting and cartridge cleaning buffer kit •
Profinia desalting purification kits (10 and 50 ml) • •
* Starter kits include histidine and GST control lysate, one 1 ml affinity cartridge, and one 10 ml desalting cartridge.

See Also
Ordering Information
Profinity IMAC Catalog # Description Price, U.S. $
resins: page 104.
Affinity Buffer Kits
6200239 Native IMAC Buffer Kit, includes native IMAC lysis, wash, and elution buffers 135.00
6200240 GST Buffer Kit, includes GST lysis, wash, and elution buffers 155.00
6200224 Desalting and Cartridge Cleaning Buffer Kit, includes desalting buffer, cleaning buffers, and cartridge storage buffer 240.00
Affinity Purification Kits
6200241 Native IMAC Purification Kit, 1 ml, includes IMAC purification buffer kit and 2 x 1 ml IMAC cartridges 255.00
6200242 Native IMAC Purification Kit, 5 ml, includes 2 IMAC purification buffer kits and 1 x 5 ml IMAC cartridge 370.00
6200243 GST Purification Kit, 1 ml, includes GST purification buffer kit and 2 x 1 ml GST cartridges 330.00
6200244
DISCONTINUED
GST Purification Kit, 5 ml, includes 2 GST purification buffer kits and 1 x 5 ml GST cartridge 515.00
Profinia Buffer Kits
6200221 Profinia Native IMAC Buffer Kit, includes lysis, wash, and elution buffers, cleaning and storage solutions; 350.00
sufficient for 10 applications
6200223 Profinia GST Buffer Kit, includes lysis, wash, and elution buffers, cleaning and storage solutions, glutathione 395.00
reagent; sufficient for 10 applications
Profinia Purification Kits
6200225 Profinia IMAC Purification Kit, 1 ml, includes Profinia native IMAC buffer kit, 2 x 1 ml IMAC and 515.00
2 x 10 ml desalting cartridges
6200235 Profinia IMAC Purification Kit, 5 ml, includes 2 Profinia native IMAC buffer kits, 1 x 5 ml IMAC 880.00
and 1 x 50 ml desalting cartridge
6200226 Profinia GST Purification Kit, 1 ml, includes Profinia GST buffer kit, 2 x 1 ml GST and 2 x 10 ml 575.00
desalting cartridges
DISCONTINUED
6200236 Profinia GST Purification Kit, 5 ml, includes 2 Profinia GST buffer kits, 1 x 5 ml GST and 1 x 50 ml 1,095.00
desalting cartridge
6200228 Profinia Desalting Purification Kit, 10 ml, includes desalting and cartridge cleaning buffer kit, 265.00
2 x 10 ml desalting cartridges
6200238 Profinia Desalting Purification Kit, 50 ml, includes 2 desalting and cartridge cleaning buffer kits, 660.00
1 x 50 ml desalting cartridge
6200229 Profinia Native IMAC Starter Kit, includes Profinia native IMAC buffer kit, 1 x 1 ml IMAC and 1 x 10 ml
desalting cartridge, E. coli lysate
6200230 Profinia GST Starter Kit, includes Profinia GST buffer kit, 1 x 1 ml GST and 1 x 10 ml desalting cartridge,
E. coli lysate, glutathione reagent
Bio-Scale Mini Affinity and Desalting Cartridges
7324610 DISCONTINUED
Bio-Scale Mini Profinity IMAC Cartridges, 5 x 1 ml 225.00
7324612 Bio-Scale Mini Profinity IMAC Cartridge, 1 x 5 ml 155.00
7324614 Bio-Scale Mini Profinity IMAC Cartridges, 5 x 5 ml 540.00
7324620 Bio-Scale Mini Profinity GST Cartridges, 5 x 1 ml 405.00
7324622 Bio-Scale Mini Profinity GST Cartridge, 1 x 5 ml 390.00
continues

108 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Discontinued products are no longer available.
 Chromatography Media
bio-rad.com/affinitypurification Affinity Chromatography

Ordering Information
Catalog # Description Price, U.S. $
Affinity Purification Kit Components
6200205 2x Native IMAC Lysis Buffer, 125 ml 78.00
6200206
 2x Native IMAC Wash Buffer 1, 125 ml 52.00
6200207 2x Native IMAC Wash Buffer 2, 100 ml 45.00
6200208 2x Native IMAC Elution Buffer, 100 ml 50.00
6200213 2x GST Lysis Buffer, 100 ml DISCONTINUED 105.00
6200215 2x GST Elution Buffer, 100 ml 58.00
6200216 5x Desalting Buffer, 200 ml 58.00
6200217 2x Cleaning Solution 1, 125 ml 52.00
6200218 4x Cleaning Solution 2, 125 ml 87.00
DISCONTINUED
6200219 2x Storage Solution, 200 ml 52.00

UNOsphere SUPrA rProtein A Media See Also

Bio-Scale Mini
cartridges:
Developed for monoclonal antibody capture ■ Designed with large pores that result in high dynamic page 132.
chromatography, UNOsphere SUPrA resin provides an binding capacities at fast flow rates EconoFit
Columns:
ideal balance between dynamic binding capacity, flow ■
 ptimized to operate under a wide range of flow rates
O page 127.
properties, and stability. The UNOsphere hydrophilic up to 600 cm/hr
polymeric support provides high-quality purifications and
For More Information
batch-to-batch reproducibility. Web: bio-rad.com/unospheresupra
Download bulletin: 6053
Key features include: Request or download bulletins: 5728 and 5729
■ Built
on robust polymeric beads engineered for high
mechanical stability, low backpressures, and resistance
to repeated clean-in-place cycles

Properties of UNOsphere SUPrA

Property Description Property Description
Composition Highly cross-linked polymer Working pH range 3–11
Particle size range 53–61 µm Clean-in-place (CIP) 6 M guanidine hydrochloride
Ligand Recombinant Protein A solutions 10 mM hydrochloric acid
0.1 M sodium hydroxide
Coupling chemistry Epoxy 1 M acetic acid/20% ethanol
Dynamic binding capacity* 30 ± 3 mg/ml at 150 cm/hour Recommended mobile 100–600 cm/hr
25 ± 2 mg/ml at 300 cm/hour phase velocity range
20 ± 2 mg/ml at 450 cm/hour
Temperature stability 2–40ºC
(Minimum spec: 20 mg/ml at 300 cm/hour)
Delivery conditions 50% slurry in 20% ethanol
Chemical stability** 10 mM hydrochloric acid
6 M guanidine hydrochloride Storage conditions 2–8ºC
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
* 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
** No significant change in chromatographic performance after storage for 24 hr at room temperature.

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 109
Discontinued products are no longer available.
 Chromatography Media
Affinity Chromatography bio-rad.com/affinitypurification

Ordering Information
Catalog # Description Price, U.S. $
UNOsphere SUPrA rProtein A Media
1560250 UNOsphere SUPrA rProtein A Media, 5 ml Inquire
1560218 UNOsphere SUPrA rProtein A Media, 25 ml Inquire
1560219 UNOsphere SUPrA rProtein A Media, 100 ml Inquire
12009321 EconoFit UNOsphere SUPrA Column, 1 x 1 ml
12009323 EconoFit UNOsphere SUPrA Column, 1 x 5 ml
12009322 EconoFit UNOsphere SUPrA Columns, 5 x 1 ml

Description 1 x 1 ml Price, U.S. $ 5 x 1 ml Price, U.S. $ 1 x 5 ml Price, U.S. $

Prepacked Bio-Scale Mini Cartridges DISCONTINUED
UNOsphere SUPrA Affinity Media 7324200 155.00 7324201 660.00 7324202 565.00

Affi-Gel and Affi-Prep Protein A Resins

Chromatography on Affi-Gel and Affi-Prep Protein A resins This is 8–10 times higher than with standard methods.
yield highly purified immunoglobulins (IgG), selectively In addition, the MAPS process and Affi-Gel Protein A
remove IgG prior to analysis of other IgG classes, or adsorb permit greater binding of mouse IgG1 than does
immune complexes for antigen purification. Protein A binds immobilized Protein G resin.
to the Fc region of immunoglobulins, especially IgG from For More Information
mammalian species. Advantages include: Web: bio-rad.com/affigelproteina
■
High purity of IgGs Capacities of Protein A Resins
■
High affinity for mammalian IgG Affi-Gel Protein A Affi-Prep Protein A
■ Highcapacities for mouse IgG1 as well as other Immunoglobulin Capacity, mg/ml Capacity, mg/ml
subclasses with MAPS optimized buffer Mouse IgG1 6–10 7–9
Mouse IgG2a 6–10 7–9
In addition, Affi-Prep resins offer high linear flow rates up Mouse IgG2b 6–10 7–9
Mouse IgG3 6–10 7–9
to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar),
Mouse IgM* 6–10 7–9
and high chemical stability, which allows sanitization with Human IgG 15 10–12
0.1 M NaOH. Sheep, cow, horse, goat,
rabbit, dog, pig IgG 8–10 7–9
With Affi-Gel and Affi-Prep Protein A resins and MAPS II
* Approximately 50% of all mouse IgMs bind using the MAPS buffer system.
buffer, you can purify up to 10 mg of IgG1/ml of media.

Ordering Information
Catalog # Description Price, U.S. $
Affi-Prep and Affi-Gel Media
1560006 Affi-Prep Protein A Media, 5 ml 415.00
1560005 Affi-Prep Protein A Media, 25 ml 1,965.00
1536153 Affi-Gel Protein A Media, 5 ml 330.00
1536154 Affi-Gel Protein A Media, 50 ml Inquire
1536159 Affi-Gel Protein A MAPS II Kit, includes 5 ml Affi-Gel Protein A media, Affi-Gel Protein A MAPS II buffers, 470.00
1 x 10 cm Econo-Column column; enough to purify 500 mg of mouse IgG1
1536161 Protein A MAPS II Binding Buffer, makes 5 L 835.00
Prepacked Econo-Pac Columns
7322022 Econo-Pac Protein A Columns, prefilled with Affi-Gel Protein A media, 5 540.00
7322020 Econo-Pac Protein A Kit, 1 x 2 ml Affi-Gel Protein A column, 1 x 10 ml 10DG column, buffers 330.00

Description 5 x 1 ml Price, U.S. $ 1 x 5 ml Price, U.S. $

DISCONTINUED
Prepacked Bio-Scale Mini Cartridges
Affi-Prep Protein A Media 7324600 655.00 7324602 555.00

Catalog # Description
12009237 EconoFit Affi-Prep Protein A Column, 1 x 5 ml
12009236 EconoFit Affi-Prep Protein A Columns, 5 x 1 ml

110 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Discontinued products are no longer available.
 Chromatography Media
bio-rad.com/affinityresin Affinity Chromatography

Affi-Gel Blue Resin

Affi-Gel Blue resin is a cross-linked agarose bead with For More Information
covalently attached Cibacron Blue F3GA dye. The blue dye Web: bio-rad.com/affigelblue
functions as an ionic, hydrophobic, aromatic, or sterically
active binding site in various applications. Affi-Gel Blue
resin is ideally suited for albumin removal (using 50–100
mesh) and enzyme purification (using 100–200 mesh).
Proteins and peptides are bound and released with a high
degree of specificity by manipulating the composition of
the eluent buffers.
The resin is available in bottle and cartridge form. Bio-Scale Mini Affi-Gel Blue Cartridge Affi-Gel Blue Gel

Ordering Information
Catalog # Description Applications (all listed products) Price, U.S. $
1537301 Affi-Gel Blue Gel, 100 ml, 50–100 mesh • Albumin removal 545.00
1537302 Affi-Gel Blue Gel, 100 ml, 100–200 mesh • Enzyme purification 545.00
153-7303 Affi-Gel Blue Gel, 1 L, 100–200 mesh 545.00
7324642 Bio-Scale Mini Affi-Gel Blue Cartridge, 1 x 5 ml, 140.00
cartridge packed with 100–200 mesh media
7324644 Bio-Scale Mini Affi-Gel Blue Cartridges,DISCONTINUED
5 x 5 ml, 480.00
cartridges packed with 100–200 mesh media
12009234 EconoFit Affi-Gel Blue Column, 1 x 5 ml
12009235 EconoFit Affi-Gel Blue Columns, 5 x 5 ml

DEAE Affi-Gel Blue Resin

DEAE Affi-Gel Blue resin is a bifunctional affinity gel For More Information
containing Cibacron Blue F3GA dye covalently attached Web: bio-rad.com/affigelDEAE
to DEAE Bio-Gel A resin. The dye binds albumin,
proteases, and other complement proteins; the DEAE
group binds remaining acidic proteins. Features of
DEAE Affi-Gel Blue resin include:
■ Single-stepIgG purification from serum; the eluted IgG
contains a small amount of transferrin (samples are
diluted approximately fivefold)
■ No detectable proteolytic activity in the eluted IgG fraction
■ Economical alternative to Protein A affinity chromatography Bio-Scale Mini DEAE Affi-Gel Blue Cartridge DEAE Affi-Gel Blue Gel
■
Available in bottle and cartridge form

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 111
Discontinued products are no longer available.
 Chromatography Media
Affinity Chromatography bio-rad.com/affinityresin

Ordering Information
Catalog # Description Applications (all listed products), U.S. $
1537307 DEAE Affi-Gel Blue Gel, 100 ml • Albumin, proteases, and complement protein removal 650.00
153-7306 DEAE Affi-Gel Blue Gel, 1 L • IgG purification 165.00
153-7308 DEAE Affi-Gel Blue Gel, 5 L 165.00
7324632 Bio-Scale Mini DEAE Affi-Gel Blue 165.00
Cartridge, 1 x 5 ml
7324634 Bio-Scale Mini DEAE Affi-Gel Blue 560.00
Cartridge, 5 x 5 ml
Description DISCONTINUED
1 x 5 ml Price, U.S. $ 5 x 5 ml Price, U.S. $
Prepacked Bio-Scale Mini Cartridges
DEAE Affi-Gel Blue Gel 7324632 7324634
Affi-Gel Blue Gel 7324642 7324644

CM Affi-Gel Blue Resin

CM Affi-Gel Blue resin contains Cibacron Blue F3GA

dye covalently coupled to CM Bio-Gel A resin.
This bifunctional gel binds both albumin and serum
proteases. CM Affi-Gel Blue chromatography provides
a convenient initial step in the purification of serum
proteins. Features of CM Affi-Gel Blue resin include:
■ Rapid removal of ≥90% of albumin and all plasminogen
in serum samples
■
No prior sample preparation needed
■ ≥80% yield of stable antiserum free of albumin
and protease activity
For More Information
CM Affi-Gel Blue Gel
Web: bio-rad.com/affigelcm

Ordering Information
Catalog # Description Price, U.S. $
1537304 CM Affi-Gel Blue Gel, 100 ml 650.00
153-7305 CM Affi-Gel Blue Gel, 1 L

112 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
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 Chromatography Media
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Affi-Gel Boronate Resin

Affi-Gel boronate-derivatized polyacrylamide resin has

affinity for coplanar adjacent cis-hydroxyl groups (cis-diols)
and a high binding capacity, which provides highly efficient
separation of low MW molecules such as nucleotides,
nucleosides, catecholamines, and sugars. It has a sorbitol
capacity of 130 µmol/ml.
For More Information
Web: bio-rad.com/affigelboronate
Download bulletin: 1066
Affi-Gel Boronate Gel

Ordering Information
Catalog # Description Price, U.S. $
1536103 Affi-Gel Boronate Gel, 5 g 650.00
153-6104 Affi-Gel Boronate Gel, 50 g Inquire

Affi-Prep Polymyxin Resin

Affi-Prep polymyxin resin consists of 2–4 mg of USP-grade

polymyxin per ml of the macroporous polymeric Affi-Prep
support. Affi-Prep polymyxin resin binds endotoxins from
a number of different strains of gram-negative bacteria
including E. coli, Salmonella abortus, S. minnesota,
and Serratia marcescens. Features of Affi-Prep polymyxin
resin include:
■ Endotoxin
removal in research and
process-scale applications
■
Linear flow rates to 2,000 cm/hr
■
Pressure stability to 1,000 psi (70 bar)
Affi-Prep Polymyxin Resin
■
 igh chemical stability (withstands sanitization with
H
0.1 M NaOH)
For More Information
Web: bio-rad.com/affigelpolymyxin

Ordering Information
Catalog # Description Price, U.S. $
1560010 Affi-Prep Polymyxin Resin, 25 ml 835.00

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 113
 Chromatography Media
Affinity Chromatography bio-rad.com/affinityresin

Affi-Gel Heparin Resin

Affi-Gel heparin resin is a ready-to-use support for the

purification of a range of proteins such as coagulation
factors, other plasma proteins, polynucleotide
polymerases, nucleases, lipases, lipoproteins, and
proteases. Heparin binds a variety of enzymes and other
proteins, either ionically or by other specific enzyme-
inhibitor (or enzyme-activator) interactions. Features of
Affi-Gel heparin resin include:
■
Heparin content ≥0.6 mg/ml
■
Human antithrombin III binding capacity ≥1.2 mg/ml
■
Linear flow rates of 10–20 cm/hr
Affi-Gel Heparin Gel
For More Information
Web: bio-rad.com/affigelheparin

Ordering Information
Catalog # Description Price, U.S. $
1536173 Affi-Gel Heparin Gel, 40 ml 525.00

Affi-Gel 10 and Affi-Gel 15 Resins

Affi-Gel 10 and Affi-Gel 15 activated affinity resins provide ■

Aqueous or anhydrous coupling conditions
spontaneous, rapid, and highly efficient coupling of ligands ■
Complete protein coupling within 4 hr at 4°C
via primary amines. Affi-Gel 10 resin is most efficient for ■
Protein coupling capacity up to 35 mg/ml of resin
coupling neutral or basic proteins with pI 6.5–11.
Affi-Gel 15 resin is recommended for coupling acidic For More Information
Web: bio-rad.com/affigel10and15
proteins with pI <6.5. Affi-Gel 10 and 15 resins offer: Download bulletin: 1085

Ordering Information
Catalog # Description Price, U.S. $
1536099 Affi-Gel 10 Media, 25 ml 175.00
1536046 Affi-Gel 10 Media, 4 x 25 ml 545.00
153-1000 Affi-Gel 10 Media, 1 L Inquire
1536051 Affi-Gel 15 Media, 25 ml 195.00
1536052 Affi-Gel 15 Media, 4 x 25 ml 615.00
1536098 Affi-Gel 10/15 Combination, includes 2 x 25 ml Affi-Gel 10 media and 2 x 25 ml Affi-Gel 15 media 560.00

114 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
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Affi-Gel Hz Hydrazide Resin

Affi-Gel Hz hydrazide activated resin couples immunoglobulin ■

Stable covalent hydrazone bonds
G (IgG) molecules via carbohydrate moieties on the Fc region ■
Mild oxidation without alteration of antibody activity
of antibody molecules. Fc attachment results in a more ■
High antigen binding capacity
specific antigen-antibody interaction and 100–300% higher ■
pH stability
antigen binding capacity than other resins. Affi-Gel Hz resin For More Information
advantages include: Web: bio-rad.com/affigelhydrazide

Ordering Information
Catalog # Description Price, U.S. $
1536047 Affi-Gel Hz Hydrazide Media, 25 ml 315.00
1536060 Affi-Gel Hz Immunoaffinity Kit, includes 5 ml Affi-Gel Hz media, 2 x 25 mg Affi-Gel Hz oxidizer, 330.00
DISCONTINUED
25 ml Affi-Gel Hz coupling buffer concentrate, 2 Econo-Pac 10DG desalting columns, 1 x 10 cm
Econo-Column column
153-6054 Affi-Gel Hz 10x Coupling Buffer Concentrate, 500 ml 150.00

Carbodiimide Activated Resin See Also

Affinity media
selection guide:
Affi-Gel 102 resin is for use with 1-ethyl-3- Affi-Gel 102 amino-terminal cross-linked agarose page 82.
(3-dimethylaminopropyl)carbodiimide hydrochloride resins with a six-atom hydrophilic arm feature:
(EDAC or EDC) carbodiimide coupling reagent, which ■
EDAC carbodiimide coupling reagent
immobilizes ligands that contain primary or terminal ■
Compatibility with carboxyl-containing ligands
carboxyl groups. The resin offers flexible alternative
For More Information
chemistries and economy.
Web: bio-rad.com/carbodiimide

Ordering Information
Catalog # Description Price, U.S. $
1532401 Affi-Gel 102 Media, 50 ml 305.00
1530990 EDAC, 5 g 200.00
DISCONTINUED

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 115
Discontinued products are no longer available.
 Chromatography Media
Size Exclusion Chromatography bio-rad.com/sizeexclusion

Size Exclusion Chromatography

See Also Bio-Gel P Media

Sample preparation
products: page 1.
Gel filtration Bio-Gel P polyacrylamide resin, for high-resolution gel
chromatography filtration, is prepared by copolymerization of acrylamide
standard: page 122.
and N,N'-methylene-bis-acrylamide. Bio-Gel P resin:
Bio-Spin and
Micro Bio-Spin ■
Is available in several particle size ranges with molecular
prepacked columns:
page 134. weight exclusion limits ranging from 100–100,000
Empty Econo-Pac ■
Is extremely hydrophilic and essentially nonionic
columns: page 141.
ENrich SEC
■
Provides efficient, gentle gel filtration of sensitive compounds
columns: page 126. ■
 oes not support microbial growth or leach
D
carbohydrates (due to its synthetic composition) as
dextrose and agarose gels can
Bio-Gel P Resin Bio-Scale Mini Bio-Gel Cartridges
Bio-Gel P resin is autoclavable at pH 5.5–6.5 and operates
over a pH range of 2–10 at room temperature. Flow rate
Bio-Gel P Polyacrylamide Resin Selection Guide
and resolution increase with increasing temperature in the
range of 4– 80°C. Media MW Fractionation Range
Bio-Gel P-2 100–1,800
Bio-Gel P polyacrylamide resin is available as bottled resin Bio-Gel P-4 800–4,000
or as prepacked spin columns and cartridges. Cartridges Bio-Gel P-6 1,000–6,000
are packed with Bio-Gel P-6 resin, while spin columns Bio-Gel P-6DG 1,000–6,000
Bio-Gel P-10 1,500–20,000
come prepacked with both P-6 and P-30 resin in either Bio-Gel P-30 2,500–40,000
Tris or SSC buffer. Bio-Gel P-60 3,000–60,000
Bio-Gel P-100 5,000–100,000
For More Information
Web: bio-rad.com/biogelp
Download bulletin: 2068

Ordering Information
Catalog # Description Comments Price, U.S. $
1504114 Bio-Gel P-2 Media, fine, 100 g Rapid carbohydrate, peptide, and protein desalting 295.00
150-4115 Bio-Gel P-2 Media, fine, 500 g 1,090.00
12002617 Bio-Gel P-2 Media, fine, 1 kg 295.00
1504118 Bio-Gel P-2 Media, extra fine, 100 g 295.00
1504120 Bio-Gel P-4 Media, medium, 100 g Carbohydrate and peptide separations, protein desalting 295.00
1504124 Bio-Gel P-4 Media, fine, 100 g 330.00
1504128 Bio-Gel P-4 Media, extra fine, 100 g 330.00
1504130 Bio-Gel P-6 Media, medium, 100 g Purification of proteins and polypeptides 260.00
1504134 Bio-Gel P-6 Media, fine, 100 g 295.00
1504138 Bio-Gel P-6 Media, extra fine, 100 g 295.00
1500738 Bio-Gel P-6DG Media, 100 g Rapid carbohydrate, peptide, and protein desalting; 295.00
150-0739 Bio-Gel P-6DG Media, 1 kg also available in prepacked columns and cartridges Inquire
1504140 Bio-Gel P-10 Media, medium, 100 g Purification of proteins and polypeptides 330.00
1504144 Bio-Gel P-10 Media, fine, 100 g 330.00
1504150 Bio-Gel P-30 Media, medium, 100 g 330.00
1504154 Bio-Gel P-30 Media, fine, 100 g 345.00
12002610 Bio-Gel P-30 Media, fine, 1 kg 345.00
1504160 Bio-Gel P-60 Media, medium, 100 g 345.00
1504164 Bio-Gel P-60 Media, fine, 100 g 345.00
12002609 Bio-Gel P-60 Media, fine, 1 kg 345.00
1504170 Bio-Gel P-100 Media, medium, 100 g 330.00
1504174 Bio-Gel P-100 Media, fine, 100 g 330.00
continues

116 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/sizeexclusion Size Exclusion Chromatography

Ordering Information

Catalog # Description
Prepacked Econo-Pac Columns
7322010 Econo-Pac 10DG Desalting Columns, 30 145.00

EconoFit Bio-Gel P-6 Desalting Columns

12009238 EconoFit Bio-Gel P-6 Desalting Column, 1 x 5 ml, column filled with Bio-Gel P-6 gel filtration
(size exclusion) chromatography support; 16 x 25 mm, maximum pressure 72 psi. For desalting or
buffer exchange
12009239 EconoFit Bio-Gel P-6 Desalting Columns, 5 x 5 ml, columns filled with Bio-Gel P-6 gel filtration
(size exclusion) chromatography support; 16 x 25 mm, maximum pressure 72 psi. For desalting or
buffer exchange
12009317 EconoFit Desalting Kit, includes 2 x 5 ml each of Bio-Gel P6 and Nuvia IMAC

Description Pack of 1 Price, U.S. $ Pack of 5 Price, U.S. $

Prepacked Bio-Scale Mini Cartridges
Bio-Gel P-6 Gel (Desalting), 5 ml DISCONTINUED
7324502 62.00 7324504 200.00
Bio-Gel P-6 Gel, 10 ml — 7325304 370.00
Bio-Gel P-6 Gel, 50 ml 7325312 365.00 7325314 1,485.00

Catalog # Description
Adaptor Fittings
7320111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system 145.00
7320112 Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge to 97.00
an HPLC system
7320113 Luer to BioLogic and NGC Systems Fittings Kit, low-pressure system fittings kit, includes 1/4–28
female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow and
NGC systems 59.00

Catalog # Description Contents MW pl For Use with Price, U.S. $

Standard
1511901 Gel Filtration Thyroglobulin 670,000 4.5 ENrich SEC 235.00
Standard, 6 vials Bovine g-globulin 158,000 5.1 columns;
Chicken ovalbumin 44,000 4.6 Bio-Gel P media
Equine myoglobin 17,000 6.9
Vitamin B12 1,350 4.5

Bio-Gel A 1.5 m Media

Bio-Gel A 1.5 m resin, ideal for purification of antibodies changing the bed volume. Bio-Gel A 1.5 m resin may
and aggregates, consists of agarose beads in which the be used at pH 4–13 and at temperatures 2–30°C.
pore size is controlled by the percentage of agarose in the The fractionation range is 10,000–1,500,000 daltons.
gel. It is compatible with all commonly used buffers and For More Information
can be used with high-salt buffers without significantly Web: bio-rad.com/biogela

Ordering Information
Catalog # Description Price, U.S. $
151-0450 Bio-Gel A 1.5 m Gel, fine, 500 ml Inquire

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 117
Discontinued products are no longer available.
 Chromatography Media
Hydrophobic Interaction Chromatography bio-rad.com/HIC

See Also Bio-Beads S-X Resins

ENrich SEC
columns: page 126.
Bio-Beads S-X resins are neutral porous styrene methylene chloride, o-dichlorobenzene, perchloroethylene,
divinylbenzene beads for size exclusion chromatography tetrahydrofuran, and trichlorobenzene.
of lipophilic polymers and other solutes that require
Recommended flow rates depend upon the cross-linkage:
organic eluents. MW exclusion limits range from
400–14,000. This range is useful for fractionation of low
■
1% cross-linked resins are used only with gravity flow
MW organic polymers and other hydrophobic substances. ■ 3% cross-linked resins can withstand a flow of 5 ml/min
Exclusion limits are influenced by the eluent used. with a backpressure of 20 bar or 300 psi
Bio-Beads S-X resins require an eluent that is mobile; ■ 8%and 12% cross-linked resins can withstand
therefore, the beads must be used in a column. The beads backpressure up to 33 bar or 500 psi
are compatible with benzene, toluene, xylene, carbon
tetrachloride, dimethylformamide, ketones, aromatics, For More Information
Web: bio-rad.com/biobeads

Ordering Information
Catalog # Description Size, µm ml/dry g* Application** Price, U.S. $
1522150 Bio-Beads S-X1 Media, 100 g 40–80 7.5 1% cross-linked; for lipophilic polymers of MW 600–14,000 465.00
152-2151 Bio-Beads S-X1 Media, 1 kg 40–80 7.5 1% cross-linked; for lipophilic polymers of MW 600–14,000 3 Inquire
1522750 Bio-Beads S-X3 Media, 100 g 40–80 4.75 3% cross-linked; for organic compounds of MW ≤2,000 465.00
152-2753 Bio-Beads S-X3 Media, 1 kg 40–80 4.75 3% cross-linked; for organic compounds of MW ≤2,000 465.00
1523350 Bio-Beads S-X8 Media, 100 g 40–80 3.1 8% cross-linked; for organic compounds of MW ≤1,000 465.00
1523650 Bio-Beads S-X12 Media, 100 g 40–80 2.5 12% cross-linked; for organic compounds of MW ≤400 540.00
Larger volumes and special packaging for industrial applications are available on request.
* Swollen in benzene.
** MW range is for beads fully swollen in benzene.

Hydrophobic Interaction Chromatography

Macro-Prep HIC Resins

Macro-Prep HIC resins are methacrylate-based beads for

protein, polypeptide, enzyme, and nucleic acid purification.
They are autoclavable and can withstand treatment in
acid, base (pH up to 10), chaotropic agents, or detergents
while retaining high protein binding capacities. They are
available in two functional forms: a weakly hydrophobic
methyl support for purification of compounds with strong
hydrophobic regions, and a mildly hydrophobic t-butyl
support for purification of compounds with few or weakly
hydrophobic regions. Both resins are chemically and
thermally stable. They can be cleaned in place with ethanol
or 1% acetic acid and 1% phosphoric acid. Macro-Prep Methyl HIC Support

For More Information

Web: bio-rad.com/macroprepHIC
Download bulletin: 1841

118 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/resinsampling Hydrophobic Interaction Chromatography

Ordering Information
Description Methyl HIC Price, U.S. $ t-Butyl HIC Price, U.S. $
Macro-Prep HIC Support, 25 ml 1580080 105.00 1580090 105.00
Macro-Prep HIC Support, 100 ml 1560080 395.00 1560090 395.00
Macro-Prep HIC Support, 500 ml 156-0081 Inquire 156-0091 Inquire
Macro-Prep HIC Support, 5 L 156-0082 Inquire ­
­—
Macro-Prep HIC Support, 10 L 156-0083 Inquire 156-0093
Catalog # Description Price, U.S. $
EconoFit Macro-Prep Columns* 465.00
12009277 EconoFit Macro-Prep Methyl HIC Column, 1 x 1 ml, 7 mm x 25 mm 465.00
12009278 EconoFit Macro-Prep t-Butyl HIC Column, 1 x 1 ml, 7 mm x 25 mm Inquire
* Maximum pressure 72 psi. Inquire

Bio-Beads SM-2 Adsorbents

Bio-Beads SM-2 nonpolar polystyrene adsorbents are

analytical-grade neutral macroporous polymeric beads with
a high surface area for adsorbing organics of MW <2,000.
These beads can be used in aqueous solution and with
solvents or solvent mixtures, including alcohols, petroleum
ether, diethyl ether, and hexane, without expansion or
contraction of the beads.
Common applications of Bio-Beads SM-2 adsorbents
include the removal of detergents such as Triton X-100, the Bio-Beads SM-2 Adsorbent

removal of organics such as polyaromatic hydrocarbons

from water, cleanup of drugs from plasma and urine, For More Information
cleanup of biological metabolites and pesticides, and Web: bio-rad.com/biobeads_SM2
cleanup of dyes and mycotoxins from food products. Download bulletin: 1461
Request or download bulletin: 6905

Ordering Information
Catalog # Description Price, U.S. $
1528920 Bio-Beads SM-2 Adsorbents, 25 g 465.00
1523920 Bio-Beads SM-2 Adsorbents, 100 g 465.00
152-3922 Bio-Beads SM-2 Adsorbents, 1 kg Inquire
152-3923 Bio-Beads SM-2 Adsorbents, 10 kg Inquire

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 119
 Chromatography Media
Chromatography Sample Packs, Kits, and Standards bio-rad.com/resinsampling

Chromatography Sample Packs, Kits, and Standards

Sample Packs

Bio-Rad offers its most popular resins in a variety of

convenient sampler packs:
■ Resinsampler pack — includes 25 ml each of
UNOsphere Q and S, Nuvia S, Macro-Prep DEAE,
High Q, and High S; 5 ml of UNOsphere SUPrA ; 10 g each
of CHT ceramic hydroxyapatite Types I and II, 40 µm;
and 10 g of CFT ceramic fluoroapatite Type II resins Resin Sampler Packs

■
 io-Scale Mini ion exchange sampler pack —
B
includes one 1 ml cartridge each of UNOsphere Q and S,
Macro-Prep High Q and High S, and DEAE resins
■
 io-Scale Mini affinity sampler pack — includes
B
one 1 ml cartridge each of Profinity IMAC, Affi-Prep
Protein A, and Profinity GST resins and one 5 ml Bio-Scale Mini Cartridges
cartridge each of DEAE Affi-Gel Blue and Affi-Gel
Blue resins
For More Information
DISCONTINUED
Web: bio-rad.com/resinsampling

Ordering Information
Catalog # Description Price, U.S. $
Process Scale Media
1580100 Resin Sampler Pack, includes 25 ml each of Macro-Prep DEAE, Macro-Prep High Q, Macro-Prep High S, 670.00
UNOsphere Q, UNOsphere S, 5 ml of UNOsphere SuPrA, 10 g each of CHT ceramic hydroxyapatite Types l
and II, 40 µm, and 10 g of CFT ceramic fluoroapatite Type II, 40 µm
Bio-Scale Mini Kits
7324650 Bio-Scale Mini Ion Exchange Sampler Pack, includes one 1 ml cartridge each of UNOsphere S, 275.00
UNOsphere Q, Macro-Prep High Q, Macro-Prep High S, Macro-Prep DEAE
7324651 Bio-Scale Mini Affinity Sampler Pack, includes one 1 ml cartridge each of Profinity IMAC, 660.00
Affi-Prep Protein A, and Profinity GST, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue

120 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Discontinued products are no longer available.
 Chromatography Media
bio-rad.com/resinsampling Chromatography Sample Packs, Kits, and Standards

Chromatography Application Kits See Also

Ion exchange resins:
page 84.
Maximize method optimization and parameter screening Macro-Prep ion
exchange media:
for biomolecules with these convenient, ready-to-use page 87.
application and resin screening kits. These kits address UNOsphere and
the entire range of needs for biomolecule purification Nuvia ion exchange
media: page 84.
from capture, intermediate contaminant removal, and final
CHT ceramic
polishing. hydroxyapatite:
page 97.
■ Convenient prepacked columns CFT ceramic
fluoroapatite: page 99.
■
Ideal for screening and optimization of purification
Macro-Prep HIC
protocols resins: page 118.
■
Reproducible and scalable results Bio-Scale Mini
cartridges: page 132.
For More Information
Web: bio-rad.com/resinsampling
Request or download bulletin: 5728

Ordering Information
Catalog # Description Price, U.S. $
7324407 Bio-Scale Mini Apatite Purification Cartridge Kit, includes one each, 5 ml prepacked CFT ceramic 335.00
fluoroapatite Type II, 40 µm and CHT ceramic hydroxyapatite Type I and II, 40 µm, multimodal
chromatography media cartridges
7324408 Bio-Scale Mini mAb Purification Cartridge Kit, includes one each, 5 ml prepacked UNOsphere SUPrA 450.00
affinity media, UNOsphere Q media, and CHT ceramic hydroxyapatite Type I, 40 µm, cartridges

EconoFit Application Kits

12009315 DISCONTINUED
EconoFit Aggregate Clearance Kit, includes one 1 ml each of Nuvia cPrime, Nuvia aPrime 4A, Nuvia HR-S, CHT XT
12009311 EconoFit Anionic IEX Kit, includes 1 ml each of Macro-Prep High Q, Macro-Prep High Q-3HT, UNOsphere Q, Nuvia Q,
Macro-Prep DEAE, and Nuvia HP-Q
12009310 EconoFit Cationic IEX Kit, includes 1 ml each of Macro-Prep High S, UNOsphere S, Nuvia S, Nuvia HR-S, and Macro-Prep CM
12009309 EconoFit CHT Kit, includes 1 ml each of CHT I, 40 µm; CHT II, 40 µm; MPC I, 40 µm; and CFT II, 40 µm
12009317 EconoFit Desalting Kit, includes 2 x 5 ml each of Bio-Gel P6 and Nuvia IMAC
12009314 EconoFit Large Biomolecule Kit, includes 1 ml each of Nuvia Q; Nuvia HP-Q; Nuvia cPrime; Nuvia aPrime 4A; CHT II, 40 µm;
and CHT XT
12009313 EconoFit mAb Kit, includes 1 ml each of Nuvia S, Nuvia Q, Nuvia cPrime, Nuvia aPrime 4A, Nuvia HR-S, and CHT XT

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 121
Discontinued products are no longer available.
 Chromatography Media
Chromatography Sample Packs, Kits, and Standards bio-rad.com/chromstandards

Chromatography Standards

Ion Exchange Chromatography Standards Gel Filtration Chromatography Standard

Bio-Rad offers two protein standards for ion exchange Bio-Rad’s gel filtration standard is a calibration standard
chromatography that are suitable for use with bulk resins, for size exclusion columns used in protein purification.
cartridges, or columns. Each standard is supplied as a The mixture includes vitamin B12 and myoglobin, which are
set of six vials of lyophilized protein mixture for qualitative visible when eluting from glass or clear plastic columns, to
analysis only. ensure that the column is properly packed and the sample
is eluting evenly. The standard can be used with most size
 rganic Acid Standard
O exclusion HPLC columns. The standard is supplied as a
Bio-Rad’s organic acid standard is supplied as a set of six set of six vials of lyophilized protein mixture.
vials of lyophilized mixture for qualitative analysis only.
For More Information
C
 arbohydrate Analysis Standard Web: bio-rad.com/chromstandards
Bio-Rad’s carbohydrate standard is supplied as a set
of six vials of lyophilized mixture for qualitative analysis
only. The standards can be used for column testing or
semiquantitative determination.

Standard Specifications
Description Contents MW pl For Use with
Protein Standard for Anion Equine myoglobin 17,000 6.9 UNO Q columns; Bio-Scale Mini
Exchange Chromatography Conalbumin 77,000 4.9 UNOsphere Q cartridges;
Chicken ovalbumin 45,000 4.6 Macro-Prep High Q and DEAE resins;
Soybean trypsin inhibitor 21,500 4.5 UNOsphere Q resin
Protein Standard for Cation Equine myoglobin 17,000 6.9 UNO S columns; Bio-Scale Mini
Exchange Chromatography Ribonuclease A 13,500 8.7 UNOsphere S cartridges;
Cytochrome c 12,000 10.7 Macro-Prep High S and CM resins;
UNOsphere S resin
Organic Acid Analysis Sodium oxalate 134 Aminex HPX-87H column;
Standard Sodium citrate 294 organic acid analysis kit
Sodium malate 196
Sodium succinate 270
Sodium formate 69
Sodium acetate 82
Carbohydrate Analysis Melezitose 504 Aminex HPX-87C column;
Standard Maltose 360 carbohydrate analysis kit
Glucose 180
Mannose 180
Fructose 180
Adonitol (ribitol) 152
Gel Filtration Standard Thyroglobulin 670,000 4.5 SEC columns; Bio-Gel P resin;
Bovine g-globulin 158,000 5.1 ENrich SEC columns
Chicken ovalbumin 44,000 4.6
Equine myoglobin 17,000 6.9
Vitamin B12 1,350 4.5

122 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Media
bio-rad.com/chromstandards Chromatography Sample Packs, Kits, and Standards

Ordering Information
Catalog # Description Contents MW pl For Use with Price, U.S. $
1250561 Protein Standard Equine myoglobin 17,000 6.9 ENrich Q Columns; UNO Q columns; 280.00
for Anion Exchange Conalbumin 77,000 4.9 Bio-Scale Mini UNOsphere Q
Chromatography, Chicken ovalbumin 45,000 4.6 cartridges; Macro-Prep High Q and
6 vials Soybean trypsin inhibitor 21,500 4.5 DEAE media; UNOsphere Q media

1250562 Protein Standard for Equine myoglobin 17,000 6.9 ENrich S Columns; UNO S columns; 280.00
Cation Exchange Ribonuclease A 13,500 8.7 Bio-Scale Mini UNOsphere S
Chromatography, Cytochrome c 12,000 10.7 cartridges; Macro-Prep High S and
6 vials CM media; UNOsphere S media
1250586 Organic Acid Sodium oxalate 134 Aminex HPX-87H column; 165.00
Analysis Standard, Sodium citrate 294 organic acid analysis kit
6 vials Sodium malate 196
Sodium succinate 270
Sodium formate 69
Sodium acetate 82
1250585 Carbohydrate Melezitose 504 Aminex HPX-87H column; 165.00
Analysis Standard, Maltose 360 carbohydrate analysis kit
6 vials Glucose 180
Mannose 180
Fructose 180
Adonitol (ribitol) 152
1511901 Gel Filtration Thyroglobulin 670,000 4.5 ENrich SEC columns; 235.00
Standard, 6 vials Bovine g-globulin 158,000 5.1 Bio-Gel P media
Chicken ovalbumin 44,000 4.6
Equine myoglobin 17,000 6.9
Vitamin B12 1,350 4.5

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 123
 Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/columns

Chromatography Columns
A complete range of prepacked and empty columns and accessories is available for protein and peptide separations.
Bio-Rad’s many disposable spin and gravity chromatography columns are made of chemically compatible polypropylene,
are autoclavable, and can be washed with NaOH. Our low-pressure glass Econo-Column chromatography columns offer an
ideal combination of performance, reliability, and value. For high-resolution separations, Bio-Rad’s Bio-Scale MT medium-
pressure columns can be run on a variety of liquid chromatography systems and are provided prepacked with selected resins.
Learn More about the Technology
Web: bio-rad.com/tech/chrom

Prepacked Chromatography Columns

Prepacked Column Selection Guide by Application
Application Media Type of Separation Chemical Form Binding Capacity/ml
Antibody/mAb UNOsphere SUPrA Affinity rProtein A Human IgG: 30 mg/ml*
Purification Affi-Prep Protein A Affinity Protein A Mouse monoclonal IgG: 8–10 mg;
human IgG: 16–23 mg
UNOsphere Q Strong anion exchange –N(CH3)3+ BSA: 180 mg/ml*
Nuvia Q Strong anion exchange –N(CH3)3+ IgG: >170 mg/ml at 300 cm/hr
Nuvia cPrime Hydrophobic cation –N(CH3)3+ Lactoferrin: >60 mg/ml; IgG: 40 mg/ml
exchange
Nuvia S Strong cation exchange –SO3– IgG: 110 mg/ml
Nuvia HR-S High-resolution strong –SO3– IgG: ≥70 mg/ml
cation exchange
CHT Type I, 40 µm Hydroxyapatite Ca5(PO4)3(OH)2 Human IgG: 25–60 mg
CHT Type II, 40 µm Hydroxyapatite Ca5(PO4)3(OH)2 Human IgG: 15–25 mg
MPC Hydroxyfluoroapatite Ca10(PO4)6(OH)1.5(F)0.5 Human IgG: 25–50 mg/ml
CFT Fluoroapatite Ca10(PO4)6F2 Lysozyme: 14–21.5 mg/g
DEAE Affi-Gel Blue Affinity Cibacron Blue F3GA Serum: 0.2–1.0 ml
and DEAE
Affinity Nuvia IMAC Affinity Nitrilotriacetic acid (NTA) Recombinant histidine-tagged protein: ≥40 mg
Protein Profinity IMAC Affinity Iminodiacetic acid Recombinant histidine-tagged protein: ≥15 mg
Purification
Profinity GST Affinity Glutathione Recombinant GST-tagged protein: ≥10 mg/ml
Profinity eXact Affinity Modified subtilisin Tag-free maltose-binding protein/ml resin:
>3 mg/ml
UNOsphere SUPrA Affinity rProtein A Human IgG: 30 mg/ml*
Protein Nuvia Q Strong anion exchange –N(CH3)3+ IgG: >170 mg/ml at 300 cm/hr
Purification Nuvia HP-Q High-throughput strong –N(CH3)3+ Thyroglobulin: ≥28 mg/ml at 150 cm/hr
anion exchange
Nuvia cPrime Hydrophobic cation –N(CH3)3+ Lactoferrin: >60 mg/ml; IgG: 40 mg/ml
exchange
Nuvia S Strong cation exchange –SO3– IgG: 110 mg/ml
Nuvia HR-S High-resolution strong –SO3– IgG: ≥70 mg/ml
cation exchange
Macro-Prep High S Strong cation exchange –SO3– Human IgG: ≥49 mg
CHT Hydroxyapatite Ca5(PO4)3(OH)2 Human IgG: 25–60 mg/ml*
MPC Hydroxyfluoroapatite Ca10(PO4)6(OH)1.5(F)0.5 Human IgG: 25–50 mg/ml
CFT Fluoroapatite Ca10(PO4)6F2 Lysozyme: 14–21.5 mg/g
High-Resolution ENrich Q Strong anion exchange –N(CH3)3+ Albumin: 130 mg/ml
Polishing ENrich S Strong cation exchange –SO3 – IgG: 120 mg/ml
ENrich SEC Size exclusion – 500–70,000 Da
5,000–650,000 Da
* Binding capacity based on bulk media; check individual instruction manuals for run conditions and specifications. continues

124 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/columns Prepacked Chromatography Columns

Prepacked Column Selection Guide by Application

Application Media Type of Separation Chemical Form Binding Capacity/ml
Desalting, Buffer Bio-Gel P-6 Gel filtration Polyacrylamide CV: 10–22%
Exchange
Purification of Affi-Gel Blue Affinity Cibacron Blue F3GA Serum: 0.2 ml;
Serum Proteins, albumin: 11 mg
Enzymes
Plasmid Macro-Prep High Q Strong anion exchange –N(CH3)3+ BSA: ≥37 mg
Purification UNOsphere Q Strong anion exchange –N(CH3)3+ BSA: 180 mg
UNOsphere S Strong cation exchange –SO3– Human IgG: 60 mg
Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 Protein: >35 mg
CHT Hydroxyapatite Ca5(PO4 )3(OH)2 Human IgG: 25–60 mg/ml*
MPC Hydroxyfluoroapatite Ca10(PO4 )6(OH)1.5(F)0.5 Human IgG: 25–50 mg/ml
CFT Fluoroapatite Ca10(PO4 )6F2 Lysozyme: 14–21.5 mg/g
* Binding capacity based on bulk media; check individual instruction manuals for run conditions and specifications.

ENrich High-Resolution Ion Exchange Columns See Also

Ion exchange
chromatography
ENrich ion exchange columns are designed for high- standards: page 122.
resolution separations at fast flow rates. ENrich columns
run at low backpressures on the NGC chromatography
system or any other medium- to high-pressure purification
system. This allows more flow rate flexibility in both
sample viscosities and temperature conditions. The 1 ml
ENrich column can complete a high-resolution separation
in about 10 minutes. ENrich columns are also available in
8 ml sizes.
The unique polymeric resin features:
■
High capacity even at high flow rates
■
Superior quality, reproducible separations ENrich glass columns — provide high-resolution
separation of biomolecules. The durable borosilicate
■
Lower backpressure than similar resins glass tube and PEEK 10-32 female end fittings are
■
Stability from pH 2–12 compatible with all aqueous solvents.
■
 vailable for strong anion (Q) and strong cation (S)
A For More Information
exchange Visit bio-rad.com/ENrich

Ordering Information
Column Recommended Column Flow Maximum Operating Pressure
Volume, Maximum Protein Dimensions
Catalog # Description ml Load, mg/column (W x L), mm psi MPa bar Price, U.S. $
ENrich High-Resolution Ion Exchange Columns
7800001 ENrich Q 5 x 50 Column 1 100 5 x 50 500 3.45 34.5 1,620.00
7800003 ENrich Q 10 x 100 Column 8 800 10 x 100 500 3.45 34.5 4,775.00
7800021 ENrich S 5 x 50 Column 1 100 5 x 50 500 3.45 34.5 1,620.00
7800023 ENrich S 10 x 100 Column 8 800 10 x 100 500 3.45 34.5 4,775.00

Catalog # Description Price, U.S. $

Fittings
7500564 Fitting Adapters 1/4"-28F to 10-32M, pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic 60.00
DuoFlow and NGC systems
7500568 UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an HPLC system, for use 47.00
with BioLogic DuoFlow and NGC systems
7800008 Fittings, 10-32 PEEK, black, 2/PK 20.00
7800091 ENrich 5 Frit Kit, includes 2 frits, 1 frit remover, 2 O-rings Inquire
7800093 ENrich 10 Frit Kit, includes 2 frits, 1 frit remover, 2 O-rings Inquire

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 125
 Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/columns

ENrich High-Resolution Size Exclusion Columns

ENrich size exclusion columns are designed for high

resolution at fast flow rates. ENrich columns run at low
backpressures on the NGC chromatography system or
any other medium- to high-pressure purification system.
This allows more flow rate flexibility in both sample
viscosities and temperature conditions. The 24 ml ENrich
column can complete a high-resolution separation in less
than 30 minutes at a flow rate of 1 ml/min.
The unique polymeric resin features:
■
High resolution based on size
■
Superior quality, reproducible separations
■
Lower backpressure than similar resins
■
Stability from pH 2–12
■
 eparation ranges from 500–70,000 and
S
5,000–650,000 Da
ENrich glass columns — provide high-resolution
separation of biomolecules. The durable borosilicate glass
tube and PEEK 10-32 female end fittings are compatible
with all aqueous solvents.
For More Information
Web: bio-rad.com/ENrich

Ordering Information
Column Separation Column Maximum Operating Pressure
Volume, Range Globular Dimensions
Catalog # Description ml Proteins, Da (W x L), mm psi MPa bar Price, U.S. $
ENrich High-Resolution Ion Exchange Columns
7801070 ENrich SEC 70 10 x 300 Column 24 500–70,000 10 x 300 600 4.1 41 1,755.00
7801650 ENrich SEC 650 10 x 300 Column 24 5,000–650,000 10 x 300 600 4.1 41 1,755.00

Catalog # Description Price, U.S. $

Fittings
7500564  itting Adapters 1/4"-28F to 10-32M, pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic
F 60.00
DuoFlow and NGC systems
7500568 UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an HPLC system, for use 47.00
with BioLogic DuoFlow and NGC systems
7800008 Fittings, 10-32 PEEK, black, 2/PK 20.00
7800093 ENrich 10 Frit Kit, includes 2 frits, 1 frit remover, 2 O-rings Inquire
Catalog # Description Contents MW pl For Use with Price, U.S. $
Standard
1511901 Gel Filtration Thyroglobulin 670,000 4.5 ENrich SEC columns; 235.00
Standard, 6 vials Bovine g-globulin 158,000 5.1 Bio-Gel P media
Chicken ovalbumin 44,000 4.6
Equine myoglobin 17,000 6.9
Vitamin B12 1,350 4.5

126 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/econofit Prepacked Chromatography Columns

EconoFit Chromatography Columns

EconoFit chromatography columns are prepacked and ■

 igh pressure rating to meet different experimental
H
easy to use with a range of Bio-Rad’s chromatography needs (72 psi)*
resins, offering scientists convenience and reliability ■
 repacked and ready-to-use formats designed to
P
from lab scale through process development. The
save time
columns’ flexible formats, high pressure rating, and easy
connection allow researchers to easily get started with For use with NGC systems or any chromatography system.
their purification.
* The pressure tolerance of the column hardware is 72 psi. The
■
 vailable in a variety of different resin formats for a range
A pressure tolerance of some packed resins is lower than 72 psi.
of purification needs For More Information
Web: bio-rad.com/econofit
■
 tandard 10-32 fittings for easy connection to many
S
different chromatography instruments

Ordering Information
Catalog # Description Price, U.S. $
12009234 EconoFit Affi-Gel Blue Column, 1 x 5 ml, prepacked affinity column, 16 x 25 mm, 275.00
maximum pressure 10 psi
12009235 EconoFit Affi-Gel Blue Columns, 5 x 5 ml, prepacked affinity columns, 16 x 25 mm, maximum pressure 10 psi 660.00
12009237 EconoFit Affi-Prep Protein A Column, 1 x 5 ml, prepacked affinity column, 16 x 25 mm, 335.00
maximum pressure 72 psi
12009236 EconoFit Affi-Prep Protein A Columns, 5 x 1 ml, prepacked affinity columns, 7 x 25 mm, 450.00
maximum pressure 72 psi
12009238 EconoFit Bio-Gel P-6 Desalting Column, 1 x 5 ml, column filled with Bio-Gel P-6 gel filtration 175.00
(size exclusion) chromatography support; 16 mm x 25 mm, maximum pressure 72 psi. For desalting or
buffer exchange
12009239 EconoFit Bio-Gel P-6 Desalting Columns, 5 x 5 ml, columns filled with Bio-Gel P-6 gel filtration 145.00
(size exclusion) chromatography support; 16 x 25 mm, maximum pressure 72 psi. For desalting or
buffer exchange
12009252 EconoFit CFT Type II, 40 µm Column, 1 x 1 ml, prepacked mixed-mode column, 7 x 25 mm, 97.00
maximum pressure 72 psi
12009240 EconoFit CFT Type II, 40 µm Column, 1 x 5 ml, prepacked mixed-mode column, 16 x 25 mm, 59.00
maximum pressure 72 psi
12009251 EconoFit CFT Type II, 40 µm Columns, 5 x 5 ml, prepacked mixed-columns, 7 x 25 mm,
maximum pressure 72 psi
12009255 EconoFit CHT Type I, 40 µm Column, 1 x 1 ml, prepacked mixed-mode column, 7 x 25 mm,
maximum pressure 72 psi
12009253 EconoFit CHT Type I, 40 µm Column, 1 x 5 ml, prepacked mixed-mode column, 16 x 25 mm,
maximum pressure 72 psi
12009254 EconoFit CHT Type I, 40 µm Columns, 5 x 5 ml, prepacked mixed-mode columns, 16 x 25 mm,
maximum pressure 72 psi
12009256 EconoFit CHT Type I, 80 µm Column, 1 x 1 ml, prepacked mixed-mode column, 7 x 25 mm,
maximum pressure 72 psi
12009259 EconoFit CHT Type II, 40 µm Column, 1 x 1 ml, prepacked mixed-mode column, 7 x 25 mm,
maximum pressure 72 psi
12009257 EconoFit CHT Type II, 40 µm Column, 1 x 5 ml, prepacked mixed-mode column, 16 x 25 mm, 275.00
maximum pressure 72 psi
12009258 EconoFit CHT Type II, 40 µm Columns, 5 x 5 ml, prepacked mixed-mode columns, 16 x 25 mm, 660.00
maximum pressure 72 psi
12009260 EconoFit CHT Type II, 80 µm Column, 1 x 1 ml, prepacked mixed-mode columns, 7 mm x 25 mm,
maximum pressure 72 psi
12009261 EconoFit CHT XT Column, 1 x 1 ml, prepacked mixed-mode column, 7 mm x 25 mm, 335.00
maximum pressure 72 psi
12009262 EconoFit DEAE Affi-Gel Blue Column, 1 x 5 ml, prepacked affinity column, 16 mm x 25 mm, 450.00
maximum pressure 10 psi
12009263 EconoFit DEAE Affi-Gel Blue Columns, 5 x 5 ml, prepacked affinity columns, 16 mm x 25 mm, 175.00
maximum pressure 10 psi
12009232 EconoFit X Empty Columns, 5 x 1 ml, empty chromatography columns, 7 mm x 25 mm, 145.00
maximum pressure 72 psi

continues

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 127
 Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/econofit

Ordering Information
Catalog # Description Price, U.S. $
12009233 EconoFit X Empty Columns, 5 x 5 ml, empty chromatography columns, 16 mm x 25 mm, 59.00
maximum pressure 72 psi
12009273 EconoFit Macro-Prep CM Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
12009274 EconoFit Macro-Prep DEAE Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
12009265 EconoFit Macro-Prep DEAE Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
12009264 EconoFit Macro-Prep DEAE Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
12009266 EconoFit Macro-Prep DEAE Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
12009275 EconoFit Macro-Prep High Q Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
12009268 EconoFit Macro-Prep High Q Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
12009267 EconoFit Macro-Prep High Q Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
12009269 EconoFit Macro-Prep High Q Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
12009283 EconoFit Macro-Prep High Q-3HT Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
12009276 EconoFit Macro-Prep High S Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
12009271 EconoFit Macro-Prep High S Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
12009270 EconoFit Macro-Prep High S Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
12009272 EconoFit Macro-Prep High S Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
12009277 EconoFit Macro-Prep Methyl HIC Column, 1 x 1 ml, prepacked hydrophobic interaction column,
7 mm x 25 mm, maximum pressure 72 psi
12009278 EconoFit Macro-Prep t-Butyl HIC Column, 1 x 1 ml, prepacked hydrophobic interaction column,
7 mm x 25 mm, maximum pressure 72 psi
12009279 EconoFit MPC I-40 Column, 1 x 1 ml, prepacked mixed-mode column, 7 mm x 25 mm,
maximum pressure 72 psi
12009280 EconoFit Nuvia aPrime 4A Column, 1 x 1 ml, prepacked hydrophobic anion exchange column,
7 mm x 25 mm, maximum pressure 45 psi
12009281 EconoFit Nuvia cPrime Column, 1 x 1 ml, prepacked hydrophobic cation exchange column,
7 mm x 25 mm, maximum pressure 45 psi
12009282 EconoFit Nuvia HP-Q Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 45 psi
12009284 EconoFit Nuvia HR-S Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 45 psi
12009288 EconoFit Nuvia IMAC Column, 1 x 1 ml, prepacked Ni-charged column, 7 mm x 25 mm,
maximum pressure 45 psi
12009286 EconoFit Nuvia IMAC Column, 1 x 5 ml, prepacked Ni-charged column, 16 mm x 25 mm, 275.00
maximum pressure 45 psi
12009285 EconoFit Nuvia IMAC Columns, 5 x 1 ml, 1 ml, prepacked Ni-charged columns, 7 mm x 25 mm, 660.00
maximum pressure 45 psi
12009287 EconoFit Nuvia IMAC Columns, 5 x 5 ml, prepacked Ni-charged columns, 16 mm x 25 mm, 335.00
maximum pressure 45 psi
12009289 EconoFit Nuvia IMAC Uncharged Column, 1 x 1 ml, prepacked uncharged IMAC column, 450.00
7 mm x 25 mm, maximum pressure 45 psi
12009290 EconoFit Nuvia Q Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm, 175.00
maximum pressure 45 psi
12009291 EconoFit Nuvia S Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm, 145.00
maximum pressure 45 psi
12009292 EconoFit Profinity eXact Column, 1 x 1 ml, prepacked affinity column, 7 mm x 25 mm,
maximum pressure 72 psi continues

128 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/econofit Prepacked Chromatography Columns

Ordering Information
Catalog # Description Price, U.S. $
12009294 EconoFit Profinity eXact Column, 1 x 5 ml, prepacked affinity column, 16 mm x 25 mm,
maximum pressure 72 psi
12009293 EconoFit Profinity eXact Columns, 5 x 1 ml, prepacked affinity columns, 7 mm x 25 mm,
maximum pressure 72 psi
12009296 EconoFit Profinity GST Column, 1 x 5 ml, prepacked affinity column, 16 mm x 25 mm,
maximum pressure 43 psi
12009295 EconoFit Profinity GST Columns, 5 x 1 ml, prepacked affinity columns, 7 mm x 25 mm,
maximum pressure 43 psi
12009297 EconoFit Profinity GST Columns, 5 x 5 ml, prepacked affinity columns, 16 mm x 25 mm,
maximum pressure 43 psi
12009299 EconoFit Profinity IMAC Column, 1 x 5 ml, prepacked Ni-charged column, 16 mm x 25 mm,
maximum pressure 72 psi
12009298 EconoFit Profinity IMAC Columns, 5 x 1 ml, prepacked Ni-charged column, 7 mm x 25 mm,
maximum pressure 72 psi
12009300 EconoFit Profinity IMAC Columns, 5 x 5 ml, prepacked Ni-charged columns, 16 mm x 25 mm,
maximum pressure 72 psi
12009307 EconoFit UNOsphere Q Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
12009302 EconoFit UNOsphere Q Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
12009301 EconoFit UNOsphere Q Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
12009303 EconoFit UNOsphere Q Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
12009308 EconoFit UNOsphere S Column, 1 x 1 ml, prepacked ion exchange column, 7 mm x 25 mm,
maximum pressure 72 psi
12009305 EconoFit UNOsphere S Column, 1 x 5 ml, prepacked ion exchange column, 16 mm x 25 mm,
maximum pressure 72 psi
12009304 EconoFit UNOsphere S Columns, 5 x 1 ml, prepacked ion exchange columns, 7 mm x 25 mm,
maximum pressure 72 psi
12009306 EconoFit UNOsphere S Columns, 5 x 5 ml, prepacked ion exchange columns, 16 mm x 25 mm,
maximum pressure 72 psi
12009321 EconoFit UNOsphere SUPrA Column, 1 x 1 ml, prepacked UNOsphere SUPrA affinity column,
7 mm x 25 mm, maximum pressure 72 psi
12009323 EconoFit UNOsphere SUPrA Column, 1 x 5 ml, prepacked UNOsphere SUPrA affinity column,
16 mm x 25 mm, maximum pressure 72 psi
12009322 EconoFit UNOsphere SUPrA Columns, 5 x 1 ml, prepacked UNOsphere SUPrA affinity column,
7 mm x 25 mm, maximum pressure 72 psi

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 129
 Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/foresight

Foresight Prepacked Plates and Columns

Foresight plates and columns are prepacked with a range

of Bio-Rad’s process chromatography resins, offering
process scientists convenience and reliability for their
high-throughput experimentation needs. The products’
robust design allows researchers to use the prepacked
formats through the entire purification development cycle,
from high-throughput resin screening to small-scale
method development and scale-up optimization.
Benefits include:
■
 repacked and ready-to-use formats designed to
P
save process development time
■
 bility to evaluate different experimental conditions to
A
better define an operational window
■
 bility to perform high-throughput experiments with
A
minimum sample requirements
■
 vailable in a variety of chromatography modes that are
A
designed for large-scale bioprocess
■
Compatible with robotic liquid handling workstations
■
 tandard 10-32 male end fittings for easy connection of
S
column to many chromatography systems
For More Information
Web: bio-rad.com/foresight
Request or download bulletin: 6297

Ordering Information
Catalog # Description Price, U.S. $
Foresight Plates*
732-4714 Foresight UNOsphere Q, 20 µl 551.25
732-4710 Foresight UNOsphere S, 20 µl 551.25
732-4703 Foresight Nuvia Q, 20 µl 551.25
732-4701 Foresight Nuvia S, 20 µl 551.25
732-4707 Foresight Nuvia HR-S, 20 µl 540.75
12006908 Foresight Nuvia HP-Q, 20 μl
12007411 Foresight Nuvia aPrime 4A, 20 µl
732-4705 Foresight Nuvia cPrime, 20 µl 551.25
732-4716 Foresight CHT Type I, 40 µm 20 µl 551.25
732-4718 Foresight CHT Type II, 40 µm, 20 µl 551.25
12003151 Foresight CHT XT, 40 µm, 20 µl
732-4785 Foresight MPC Type I, 40 µm, 20 µl 551.25
12004035 Foresight Nuvia IMAC Ni-Charged, 20 µl
continues

130 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/foresight Prepacked Chromatography Columns

See Also
Ordering Information
Ion exchange
Description 1 ml 5 ml chromatography
standards: page 122.
Foresight Columns
Foresight UNOsphere Q 732-4732 732-4752
Foresight UNOsphere S 732-4730 732-4750
Foresight Nuvia Q 732-4721 732-4741
Foresight Nuvia S 732-4720 732-4740
Foresight Nuvia HR-S 732-4723 732-4743
Foresight Nuvia HP-Q 12007020 12007021
Foresight Nuvia aPrime 4A 12007392 12007393
Foresight Nuvia cPrime 732-4722 732-4742
Foresight CHT Type I, 40 µm 732-4735 732-4755
Foresight CHT Type II, 40 µm 732-4736 732-4756
Foresight CHT XT, 40 µm 12003150 12003149
Foresight MPC Type I, 40 µm 732-4737 732-4757
Foresight Nuvia IMAC Ni-Charged 12004038 12004037
Description 200 µl 600 µl
Foresight RoboColumn Units**,***
Foresight UNOsphere Q 732-4819 732-4820
Foresight UNOsphere S 732-4813 732-4814
Foresight Nuvia Q 732-4804 732-4805
Foresight Nuvia S 732-4801 732-4802
Foresight Nuvia HR-S 732-4831 732-4832
Foresight Nuvia HP-Q 12007013 12007014
Foresight Nuvia aPrime 4A 12007394 12007395
Foresight Nuvia cPrime 732-4807 732-4808
Foresight CHT Type I, 40 µm 732-4822 732-4823
Foresight CHT Type II, 40 µm 732-4825 732-4826
Foresight CHT XT, 40 µm 12003152 12003148
Foresight MPC Type I, 40 µm 732-4828 732-4829
Foresight Nuvia IMAC Ni-Charged 12004051 12004052

Catalog# Description
Fittings
7601311 Long Fingertight Fittings, 10-32 x 1.03", for connecting 1/16" PEEK and Tefzel tubing to an FPLC system, 4
7500564 Fitting Adapters 1/4"-28F to 10-32M, pkg of 2, fittings to connect one HPLC column (10-32) to BioLogic
DuoFlow and NGC systems

* Package size: 2 x 96-well plates.

** Package size: 1 row of 8 columns.
*** Foresight RoboColumn units are to be used with robotic liquid handling workstations.
For more information on prepacked columns and plates, please visit bio-rad.com/foresight.

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 131
 Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/cartridges

See Also Bio-Scale Mini Cartridges

Bottled resins:
page 81.
BioLogic LP Bio-Scale Mini cartridges have a double-wall cartridge
systems: page 170. design that provides extra durability and allows easy,
reliable runs at pressures up to 45 psi using the aqueous
buffers most commonly employed for protein separation.
The cartridges are prepacked with Bio-Rad’s
chromatography resins for ion exchange, affinity,
size exclusion, or hydroxyapatite technology and are
available in 1, 5, 10, and 50 ml formats. Bio-Scale Mini
cartridges are convenient and ready to use.
The cartridges can be used with any chromatography
system and are ideal with the NGC chromatography
systems, BioLogic systems, or a peristaltic pump. For For More Information
Web: bio-rad.com/cartridges
simple step elution, the cartridges can be used with a Download bulletins: 5444, 5574, and 5584
luer lock syringe. Fittings are available for connection to
HPLC and FPLC systems.
Bio-Scale Mini Nuvia IMAC cartridges are 5 ml Bio-Scale
Mini cartridges filled with Nuvia Ni-charged IMAC resin.
They are used for the purification of recombinant histidine
(His)-tagged proteins by immobilized metal affinity
chromatography (IMAC).

Ordering Information
Description 1 x 1 ml Price, U.S. $ 5 x 1 ml Price, U.S. $ 1 x 5 ml Price, U.S. $ 5 x 5 ml Price, U.S. $
Prepacked Cartridge Media Type
UNOsphere Q — — 7324100 210.00 7324102 150.00 7324104 505.00
UNOsphere S — — 7324110 210.00 7324112 150.00 7324114 505.00
UNOsphere SUPrA rProtein A 7324200 155.00 7324201 660.00 7324202 565.00 — —
Nuvia S 7324420 69.00 7324421 215.00 7324422 180.00 7324423 700.00
Macro-Prep High Q — — 7324120 210.00 7324122 80.00 7324124 290.00
Macro-Prep High S — — 7324130 210.00 7324132 80.00 7324134 290.00
Macro-Prep DEAE — — 7324140 235.00 7324142 80.00 7324144 300.00
Affi-Prep Protein A — — 7324600 655.00 7324602 555.00 — —
Nuvia IMAC — DISCONTINUED
— — — 7800811 160.00 7800812 600.00
Profinity IMAC Ni-Charged — — 7324610 225.00 7324612 155.00 7324614 540.00
Profinity GST — — 7324620 405.00 7324622 390.00 7324624 1,370.00
DEAE Affi-Gel Blue — — — — 7324632 165.00 7324634 560.00
Affi-Gel Blue — — — — 7324642 140.00 7324644 480.00
CHT Type I, 40 µm — — — — 7324322 155.00 7324324 615.00
CHT Type II, 40 µm — — — — 7324332 155.00 7324334 615.00
CFT Type II — — — — 7324405 155.00 7324406 615.00

Description Pack of 1 Price, U.S. $ Pack of 5 Price, U.S. $

Bio-Gel P-6, 10 ml — 7325304 370.00
Bio-Gel P-6, 50 ml 7325312 365.00 7325314 1,485.00

Catalog # Description
Adaptor Fittings
7885010 Luer to 10-32 Adaptor Fittings Kit, includes female slip luer to female 10-32 fitting to connect male end of luer 175.00
column to NGC system
7320111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system 145.00
7320112 Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system 97.00
or NGC system
7320113 Luer to BioLogic and NGC Systems Fittings Kit, low-pressure system fittings kit, includes 1/4–28 59.00
female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow and
NGC systems

132 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Discontinued products are no longer available.
 Chromatography Columns
bio-rad.com/cartridges Prepacked Chromatography Columns

Bio-Scale Mini Cartridge Purification Sampler Packs and Kits See Also
Ion exchange
chromatography
Protein purification sampler packs offer a quick way The Bio-Scale Mini mAb purification cartridge kit includes standards: page 122.
to experiment with separation techniques when the the three most commonly used media for purification of
protein to be purified has not yet been characterized. monoclonal antibody. The kit includes a UNOsphere SUPrA
affinity cartridge for capture, a UNOsphere Q cartridge for
The Bio-Scale Mini ion exchange sampler pack includes a
the intermediate step, and a CHT Type I, 40 µm cartridge
1 ml UNOsphere Q cartridge, 1 ml UNOsphere S cartridge,
for the polishing step.
1 ml Macro-Prep Q cartridge, 1 ml Macro-Prep S cartridge,
and 1 ml Macro-Prep DEAE cartridge. A list of all cartridges is included above in Ordering
Information for Bio-Scale Mini cartridges. For a list of all
The Bio-Scale Mini affinity sampler pack includes a
prepacked columns, see page 124.
1 ml Profinity IMAC cartridge, 1 ml Profinity GST cartridge,
1 ml Affi-Prep Protein A cartridge, 5 ml DEAE Affi-Gel Blue For More Information
Web: bio-rad.com/resinsampling
cartridge, and 5 ml Affi-Gel Blue cartridge.

Ordering Information
Catalog # Description Price, U.S. $
Bio-Scale Mini Cartridge Purification Sampler Packs and Kits
7324650 Bio-Scale Mini Ion Exchange Sampler Pack, includes one 1 ml cartridge each of UNOsphere S, 275.00
UNOsphere Q, Macro-Prep High Q, Macro-Prep High S, Macro-Prep DEAE
7324651 Bio-Scale Mini Affinity Sampler Pack, includes one 1 ml cartridge each of Profinity IMAC, Affi-Prep Protein A, 660.00
Profinity GST, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue
7324407
DISCONTINUED
Bio-Scale Mini Apatite Purification Cartridge Kit, includes one each of prepacked CFT ceramic 335.00
fluoroapatite Type II, 40 µm, and CHT ceramic hydroxyapatite Type I and II, 40 µm, mixed-mode
chromatography media cartridges, 1 x 5 ml each
7324408 Bio-Scale Mini mAb Purification Cartridge Kit, includes one each, 5 ml prepacked UNOsphere SUPrA 450.00
affinity media, UNOsphere Q media, and CHT ceramic hydroxyapatite Type I, 40 µm, cartridges
Adaptor Fittings
7885010 Luer to 10-32 Adaptor Fittings Kit, includes female slip luer to female 10-32 fitting to connect male end of 175.00
luer column to NGC system
7320111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system 145.00
7320112 Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system 97.00
or NGC system
7320113 Luer to BioLogic and NGC Systems Fittings Kit, low-pressure system fittings kit, includes 1/4–28 59.00
female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow and
NGC systems

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 133
Discontinued products are no longer available.
 Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/biospin

See Also Bio-Spin and Micro Bio-Spin Columns

Nucleic acid sample
preparation: page 9.
Bio-Spin P-6 and Micro Bio-Spin P-6 columns are ideal
End cap End cap
for desalting protein samples quickly using size exclusion
chromatography. Filled with Bio-Gel P-6 or P-30 resin, Reservoir Reservoir

these columns are shipped fully hydrated in Tris buffer

3 cm
and are ready to use. These products effectively clean up
5 cm 2 cm working bed height
and remove salts, nucleotides, dye terminators, and small
molecules from protein, RNA, and DNA samples in just 3.7 cm working bed height 0.8 ml bed
10 minutes. The columns are autoclavable. volume

1.2 ml bed volume

■
 rovide fast salt and contaminant removal in an
P
easy-to-use spin-column format Porous 30 µm
polyethylene bed Luer end
■
 emove compounds <6 kD by size exclusion
R support retains fitting with
fine particles Micro Bio-Spin snap-off tip
chromatography column
■
Accommodate up to 100 µl of sample (Bio-Spin columns) Luer end fitting
with snap-off tip
■
 ccommodate up to 70 µl of sample
A Bio-Spin column
(Micro Bio-Spin columns) For More Information
Web: bio-rad.com/biospin

Bio-Spin Column Selection Guide

Bio-Spin P-6 Micro Bio-Spin P-6 Bio-Spin P-30 Micro Bio-Spin P-30
Equilibration buffer SSC buffer* 10 mM Tris, pH 7.4, or SSC buffer* SSC buffer* 10 mM Tris, pH 7.4, or SSC buffer*
Applications Desalting and buffer Desalting and buffer Desalting; nucleotide DNA sequencing reaction
exchange exchange and small molecule mixtures (Tris) and small
removal molecule removal
Retention and 90% recovery of 20 bases 90% recovery of 20 bases 95% recovery of 22 bases or 95% recovery of 22 bases or
recovery or bp, 99% retention of salts or bp, 99% retention of salts bp, 98% retention of ddNTPs bp, 98% retention of ddNTPs
MW exclusion limit, 6,000 6,000 40,000 40,000
globular proteins
Sample volume 50–100 µl 10–75 µl 50–100 µl 10–75 µl
Centrifuge type Swinging bucket Microcentrifuge Swinging bucket Microcentrifuge
* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0.

Ordering Information
Catalog # Description Price, U.S. $
7326227 Bio-Spin P-6 Columns, 25 with Tris buffer 135.00
7326228 Bio-Spin P-6 Columns, 100 with Tris buffer 455.00
7326221 Micro Bio-Spin P-6 Columns, 25 with Tris buffer 110.00
7326222 Micro Bio-Spin P-6 Columns, 100 with Tris buffer 365.00
7326006 Bio-Spin P-30 Columns, 25 with SSC buffer 155.00
7326202 Micro Bio-Spin P-30 Columns, 25 with SSC buffer 110.00
7326203 Micro Bio-Spin P-30 Columns, 100 withDISCONTINUED
SSC buffer 365.00
Go to bio-rad.com/cartridges for current information on prepacked cartridges.

134 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Discontinued products are no longer available.
 Chromatography Columns
bio-rad.com/columns Prepacked Chromatography Columns

Mini Bio-Spin Columns See Also

ProteoMiner protein
enrichment system:
Mini Bio-Spin columns are available with 0.6 ml prepacked page 7.
resin for both affinity-tagged purification and protein
enrichment applications. The columns contain Profinity
eXact resins for affinity-tagged purification and on-column
cleavage; see page 106.
For More Information
Web: bio-rad.com/minibiospin
Download bulletins: Profinity eXact fusion-tag system — 5725, 5742,
and 5766; ProteoMiner beads — 3096 and 5635

Ordering Information
Catalog # Description Price, U.S. $
1563007* Profinity eXact Mini Spin Columns, includes ten 0.6 ml spin columns, ten 2 ml capped tubes, and ten 165.00
2 ml capless tubes
* For ProteoMiner kits, see page 7.

Poly-Prep Ion Exchange Columns

Poly-Prep prepacked columns for gravity-flow End cap

chromatography provide convenience for sample
preparation and other small-scale applications.
The graduated polypropylene columns hold a standard
10 ml ­reservoir
bed volume of 2 ml of AG ion exchange resin and include
an integral 10 ml reservoir. A Poly-Prep stack cap, which 9 cm
allows connection to pumps, reservoirs, 2 ml bed v­ olume
or columns in series, is also available.
Graduated
For More Information volume ­markings
Web: bio-rad.com/polyprep Porous 30 µm
polyethylene
bed s­ upport retains Luer end fitting
fine ­particles with snap-off tip

Ordering Information
Particle Ionic
Catalog # Description Size, µm Form Application Price, U.S. $
7316211 Poly-Prep Columns, AG 1-X8 106–180 Chloride Separation of low molecular weight inorganic anions 245.00
resin, 100–200 mesh, 50
7316212 Poly-Prep Columns, AG 1-X8 45–106 Chloride For high-resolution random separations 245.00
resin, 200–400 mesh, 50
7316221 Poly-Prep Columns, AG 1-X8 45–106 Formate Separation of low molecular weight biological compounds 245.00
resin, 200–400 mesh, 50 such as nucleotides, peptides, and carboxylic acids
7316213 Poly-Prep Columns, AG 50W-X8, 106–250 Hydrogen Separation and concentration of low molecular weight 245.00
100–200 mesh, 50 cations such as small peptides and amino acids
7316214 Poly-Prep Columns, AG 50W-X8, 63–150 Hydrogen For high-resolution random separations 250.00
200–400 mesh, 50
7311555 Poly-Prep Column Stack Cap, 50 Allows connection of pumps, reservoirs, or columns in series 43.00
7328102 Stopcock, Lock 2-Way Luer, 10 Provides control of flow through Poly-Prep columns 55.00

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 135
 Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/columns

See Also Bio-Scale CHT Type I Columns

Bio-Scale
replacement parts:
page 147. Bio-Scale CHT Type I columns are packed with CHT ceramic
NGC systems: hydroxyapatite, Type I 10 µm resin, which has high affinity
page 148.
for basic proteins and lower affinity for acidic proteins.
CHT ceramic
hydroxyapatite: These prepacked columns allow rapid, reproducible
page 97. high-resolution separations for analytical to semipreparative
Bio-Scale Mini medium-pressure applications. These columns are ideal for
cartridges: of the bed can be removed and replaced with fresh
page 132. use with any medium- to high-pressure chromatography
support. The Bio-Scale CHT Type I column top-off support
system. Available bed volumes are 2, 5, 10, and 20 ml.
kit contains 1 ml support, frits, and distribution screens for
Bio-Scale CHT Type I Column Top-Off Support Kit each column diameter.
If the top of the column bed becomes fouled and the usual For More Information
hygiene steps do not restore performance, a few milliliters Web: bio-rad.com/bioscaleCHT

Ordering Information
Recommended Recommended Column Maximum
Column Flow Rate, Flow Rate, Dimensions Operating
Catalog # Description Volume, ml ml/min ml/min (W x L), mm Pressure, psi Price, U.S. $
7510021 Bio-Scale CHT2-I Column 2 20 0.5–3.0 7 x 52 1,000 1,140.00
7510023 Bio-Scale CHT5-I Column 5 50 0.5–5.0 10 x 64 750 1,795.00
7510025 Bio-Scale CHT10-I Column 10 100 0.5–7.0 12 x 88 600 2,910.00
7510027 Bio-Scale CHT20-I Column 20 200 0.5–10.0 15 x 113 500 4,485.00
7510029 CHT-I Top-Off Support Kit, 400.00
1 ml

See Also UNO Monolith Ion Exchange Columns

UNOsphere ion
exchange resin:
page 84. UNO monolith ion exchange columns contain a patented*
NGC systems: continuous-bed matrix, allowing biomolecule separations
page 148.
at high flow rates without sacrificing resolution or capacity.
UNO columns run at low backpressures on medium- to
high-pressure chromatography systems. A 1 ml UNO
column can complete a high-resolution separation in
about 3 minutes. UNO columns are also available in 6
and 12 ml sizes.
The unique homogeneous UNO matrix:
■
 rovides large pore diameters especially suited for
P UNO replacement columns — UNO replacement columns
purifying larger proteins, DNA, and virus provide a simple bed replacement as an alternative to
■ Hashigh capacity, even at high flow rates, due to the purchasing a new column.
dense network of nodules that contain the ionic functional UNO glass columns — UNO glass columns provide high-
groups. These groups are completely accessible to resolution separation of biomolecules. The transparent glass
biomolecules via the interconnecting channels tube allows easy bed inspection and column troubleshooting.
■
Has the highest quality and batch-to-batch reproducibility Three column sizes provide flexibility for purification protocols.
■
Prevents fragmentation so columns last longer UNO polishing PEEK columns — UNO polishing columns,
■
Is stable from pH 2–12 0.16 ml, are a late-stage purification tool to obtain the highest
■ Is
available for strong anion (Q) and strong resolution and recovery from small sample loads. They allow
cation (S) exchange you to purify and concentrate dilute samples in one step.
For More Information
* U.S. patent 6,423,666. Web: bio-rad.com/unomonolith

136 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/aminex Prepacked Chromatography Columns

See Also
Ordering Information
Ion exchange
Column Recommended Recommended Column Flow Maximum Operating chromatography
Volume, Maximum Protein Flow Rate, Dimensions Pressure standards: page 122.
Catalog # Description ml Load, mg/column ml/min (W x L), mm psi MPa bar Price, U.S. $
7200001 UNO Q1 Column 1.3 20 0.5–5.0 7 x 35 700 4.5 48 1,760.00
7200003 UNO Q6 Column 6 90 0.5–8.0 12 x 53 700 4.5 48 3,835.00
7200005 UNO Q12 Column 12 180 0.5–8.0 15 x 68 700 4.5 48 5,680.00
7200021 UNO S1 Column 1.3 20 0.5–5.0 7 x 35 700 4.5 48 1,760.00
7200023 UNO S6 Column 6 90 0.5–8.0 12 x 53 700 4.5 48 3,835.00
7200025 UNO S12 Column 12 180 0.5–8.0 15 x 68 700 4.5 48 5,680.00
UNO Replacement Columns
7200011 UNO Q1R Column 1.3 20 0.5–5.0 7 x 35 700 4.5 48 965.00
7200013 UNO Q6R Column 6 90 0.5–8.0 12 x 53 700 4.5 48 2,835.00
7200015 UNO Q12R Column 12 180 0.5–8.0 15 x 68 700 4.5 48 2,880.00
7200031 UNO SR1 Column 1.3 20 0.5–5.0 7 x 35 700 4.5 48 965.00
7200033 UNO S6R Column 6 90 0.5–8.0 12 x 53 700 4.5 48 2,705.00
7200035 UNO S12R Column 12 180 0.5–8.0 15 x 68 700 4.5 48 2,870.00
UNO Polishing PEEK Column
7200009 UNO Q Polishing Column 0.16 2 0.1–1.0 4.6 x 10 200 1.3 14 610.00
7200029 UNO S Polishing Column 0.16 DISCONTINUED
2 0.1–1.0 4.6 x 10 200 1.3 14 635.00

Catalog # Description Price, U.S. $

Fittings
7500554 1/16" OD (1.6 mm) Post-Pump Fittings, includes Delrin nut, ferrules, lock ring, 10 sets 185.00
7500568 UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an HPLC system, for use with 47.00
BioLogic DuoFlow and NGC systems
7500567 UNO M6 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an FPLC system 36.00

Aminex HPLC Columns See Also

Organic acid
standard: page 122.
Aminex HPLC columns are packed with a polystyrene Carbohydrate
divinylbenzene resin. Aminex resin has high pressure analysis standard:
page 122.
stability, wide pH stability, and high column efficiency and
NGC systems:
selectivity. Aminex HPLC columns separate compounds page 148.
using the ion-moderated partition chromatography
technique. Aminex columns are often used in the food,
beverage, and biofuel industries. Aminex columns are
an industry standard for the analysis of carbohydrates,
organic acids, organic bases, and other small organic
molecules, including peptides and nucleic acids.
To separate complex mixtures with Aminex columns,
simple isocratic mobile phases (often just water) and
precise temperature control are used.
Aminex columns are commonly used for USP methods
under the "L" specification. Specific designations are listed
in the table below.
For More Information
Web: bio-rad.com/aminex
Download bulletins: 1928 and 6333

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 137
Discontinued products are no longer available.
 Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/aminex

Ordering Information
Particle Cross-
Guard Size, Ionic Linkage pH USP
Catalog # Description Column* Applications µm Form % Range Number Price, U.S. $
Carbohydrate Analysis Columns
1250143 Aminex HPX-87N 1250508 Beet sugars 9 Sodium 8 5–9 L58 1,430.00
Column, 300 x 7.8 mm
1250142 Aminex HPX-87K 1250128 Molasses, 9 Potassium 8 5–9 — 1,430.00
Column, 300 x 7.8 mm corn syrup
1250095 Aminex HPX-87C 1250128 High fructose 9 Calcium 8 5–9 L19 1,395.00
Column, 300 x 7.8 mm corn syrup
1250094 Aminex HPX-87C 1250128 Sugar alcohols 9 Calcium 8 5–9 L19 1,045.00
Column, 250 x 4.0 mm
1250098 Aminex HPX-87P 1250119 or Pentose sugars, 9 Lead 8 5–9 L34 1,565.00
Column, 300 x 7.8 mm 1250118 cellulose
hydrolysates,
biofuels
1250140 Aminex HPX-87H 1250129 Sugars with 9 Hydrogen 8 1–3 L17 1,430.00
Column, 300 x 7.8 mm organic acids,
biofuels
1250096 Aminex HPX-42C 1250128 Oligosaccharides, 25 Calcium 4 5–9 — 1,360.00
Column, 300 x 7.8 mm thickening agents
1250097 Aminex HPX-42A 1250118 Oligosaccharides 25 Silver 4 6–8 — 1,805.00
Column, 300 x 7.8 mm
1250105 Fast Carbohydrate 1250119 or Glucose, 9 Lead 8 5–9 — 1,240.00
Column, 300 x 7.8 mm 1250118 galactose,
sucrose, fructose
Organic Acid and Alcohol Columns
1250140 Aminex HPX-87H 1250129 Sugars with 9 Hydrogen 8 1–3 L17 1,430.00
Column, 300 x 7.8 mm organic acids,
fermentation
monitoring,
biofuels
1250100 Fast Acid Analysis 1250129 Alcohol, 9 Hydrogen 8 1–3 — 1,135.00
Column, 100 x 7.8 mm glycol organic
acid analysis
1250115 Fermentation 1250129 Sugar, acids, 9 Hydrogen 8 1–3 — 1,295.00
Monitoring Column, alcohols
150 x 7.8 mm
Application Kits for Food Analysis**
1250234 Organic Acid Included Organic acids 9 Hydrogen 8 1–3 — 1,530.00
Analysis Kit, includes DISCONTINUED
1250140, 1250129, and
1250586

Ionic pH
Catalog # Description Applications Form Range Price, U.S. $
Micro-Guard Cartridges
1250118*** De-Ashing Refill Cartridges, Aminex silver- and lead-form H+ and 6–8 340.00
30 x 4.6 mm, 2 columns CO3–
1250119 Carbo-P Refill Cartridges, Aminex HPX-87P column Lead 5–9 450.00
30 x 4.6 mm, 2
1250128 Carbo-C Refill Cartridges, Aminex calcium- and Calcium 5–9 310.00
30 x 4.6 mm, 2 potassium-form columns
1250129 Cation H Refill Cartridges, Aminex hydrogen-form Hydrogen 1–3 320.00
30 x 4.6 mm, 2 columns
1250506 Anion CO3 – Cartridges, Aminex silver- and lead-form CO3– — 510.00
30 x 4.6 mm, 2 columns
1250508 Cation Na+ Cartridges, Aminex HPX-87N column Sodium 5–9 365.00
30 x 4.6 mm, 2
continues

138 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Discontinued products are no longer available.
 Chromatography Columns
bio-rad.com/columns Prepacked Chromatography Columns

Ordering Information
Catalog # Description Contents MW pl For Use with Price, U.S. $
Standards
1250585 Carbohydrate Melezitose 504 Aminex HPX-87H column; 165.00
Analysis Standard, Maltose 360 carbohydrate analysis kit
6 vials Glucose 180
Mannose 180
Fructose 180
Adonitol (ribitol) 152
1250586 Organic Acid Sodium oxalate 134 Aminex HPX-87H column; 165.00
Analysis Standard, Sodium citrate 294 organic acid analysis kit
6 vials Sodium malate 196
Sodium succinate 270
Sodium formate 69
Sodium acetate 82
Catalog # Description Price, U.S. $
Cartridge Holders and Accessories
1250131 Standard Cartridge Holder, for one 30 x 4.6 mm cartridge 695.00
1250147 Cartridge Holder Seal Replacement Kit, for #1250131 290.00
1250148 Cartridge Holder Seals, for #1250131 135.00
1250139 De-Ashing Cartridge Holder, for #1250118, holds two 3.0 x 4.6 mm cartridges in series 875.00
7800008 Fittings, 10-32 PEEK, black, 2/PK 20.00
* Supplied as 2 guard columns.
** Includes 1 column and 2 Micro-Guard cartridges; requires standard cartridge holder, catalog #1250131.
*** Requires de-ashing cartridge holder, #1250139.

Econo-Pac Affinity and Desalting Columns See Also

Affi-Gel Protein A
MAPS II kit: page 110.
Econo-Pac prepacked columns for gravity-flow End cap
Affi-Gel Protein A
chromatography allow fast and easy desalting as well resin: page 110.
as simplified antibody purification. DEAE Affi-Gel Blue
resin: page 111.
Econo-Pac Protein A columns are well suited for binding
of lgGs, especially from mammalian species. The Econo-Pac 20 ml ­reservoir
10DG media, with a molecular exclusion limit of 6,000, is
recommended specifically for desalting and buffer exchange. Upper porous
14 cm
­polymer bed
The prepacked Econo-Pac columns include an upper frit, support
a snap-off end tip, graduated column markings, a 10 ml
bed, and 30 ml total column volume. To improve column
10 ml bed ­volume
performance, use a flow adaptor, see below. For bottled
size exclusion resins, see pages 116–117; for bottled Lower porous
affinity resins, see pages 102–106 and 109–115; and polymer
for activated affinity resins, see pages 105 and 114–115. bed support

Luer end fitting

with snap-off tip

Ordering Information
Catalog # Description Price, U.S. $
7322022 Econo-Pac Protein A Columns, prefilled with Affi-Gel Protein A media, 5 540.00
7322020 Econo-Pac Protein A Kit, 1 x 2 ml Affi-Gel Protein A column, 1 x 10 ml 10DG column, buffers 330.00
7322027 Econo-Pac Serum IgG Purification Kit, 5 DISCONTINUED
x 5 ml DEAE Affi-Gel Blue columns, 5 x 10 ml 10DG columns, buffers 405.00
7322010 Econo-Pac 10DG Desalting Columns, 30 195.00
7380019 Econo-Pac Flow Adaptor, 1.5 cm column ID 140.00

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 139
Discontinued products are no longer available.
 Chromatography Columns
Empty Columns bio-rad.com/spincolumns

Empty Columns
Bio-Rad’s empty columns accommodate a range of chromatography needs, including spin columns, low-pressure
columns with optional flow adaptors and jackets for temperature control, and analytical columns for medium- or
high-pressure systems.

See Also Bio-Spin, Micro Bio-Spin, and Mini Bio-Spin Columns

Nucleic acid sample
preparation: page 9.
Prepacked Bio-Spin Empty Bio-Spin and Micro Bio-Spin chromatography resin respectively, and fit in standard microfuges. All three
and Micro Bio-Spin columns are disposable polypropylene spin columns that columns fit standard collection tubes, have snap-off tips
columns: page 134.
can be packed with a variety of chromatographic media. and polyethylene bed supports, and are autoclavable.
Bio-Spin columns hold up to 1.2 ml of any resin and fit For More Information
in standard swinging bucket centrifuges; Micro Bio-Spin Web: bio-rad.com/spincolumns
and Mini Bio-Spin columns hold up to 0.8 and 1.2 ml of Download bulletin: 2289

End cap

Reservoir

5 cm
End cap
Reservoir 3.7 cm working bed height
Reservoir
3.3 cm
3 cm 2.8 cm working bed height 1.2 ml bed volume
2 cm working bed height
1.2 ml bed volume Porous 30 µm
0.8 ml bed volume polyethylene bed
support retains
fine particles
Reversible luer end
Luer end fitting fitting with snap-off tip Luer end fitting
with snap-off tip with snap-off tip
Micro Bio-Spin Column Mini Bio-Spin Column Bio-Spin Column

Ordering Information
Catalog # Description Price, U.S. $
7326008 Bio-Spin Chromatography Columns, empty, 100 170.00
7326025 Bio-Spin Chromatography Columns, empty, 1,000 1,540.00
7326204 Micro Bio-Spin Chromatography Columns, empty, 100 145.00
7326207 Mini Bio-Spin Chromatography Columns, empty, 100 Inquire
7311660 End Caps, for Micro Bio-Spin chromatography columns, 1,000 225.00

140 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/econopaccolumns Empty Columns

Econo-Pac Columns See Also

Prepacked
Econo-Pac columns:
Econo-Pac columns are 14 cm high, 1.5 x 12 cm polypropylene page 139.
End cap
columns that may be fitted with a flow adaptor (see below)
10 ml ­reservoir
or used for gravity-flow chromatography. When used for open
column work, a special upper bed support prevents the bed
from running dry. Bed volumes from 1–20 ml are acceptable. Graduated volume ­markings

These columns can be autoclaved and will retain fine particles.

Columns can be easily stored in poly column racks.
14 cm
For More Information 11.5 cm working bed height
Web: bio-rad.com/econopaccolumns
Download bulletin: 2289
0–20 ml bed volume

Porous 30 µm polyethylene bed

support retains fine particles
Poly Column Rack 1.5 cm ID
Luer end fitting with snap-off tip

Ordering Information
Catalog # Description Price, U.S. $
7321010  cono-Pac Chromatography Columns, empty, includes upper bed supports, end caps, tip closures, 50
E 145.00
7321011 Econo-Pac Chromatography Columns, empty, includes upper bed supports, end caps, tip closures, 500 1,330.00
Accessories
7380019 Econo-Pac Flow Adaptor, 1.5 cm column ID 140.00
7317005 Poly Column Rack, 20-place, with removable tube rack 450.00
7328102 2-Way Stopcocks, female-to-male luer, 10 55.00
7318232 Female Luer Plugs, 25, polypropylene 37.00
7311660 End Caps, for Econo-Pac columns, 1,000 225.00

Poly-Prep Columns

Poly-Prep columns are 9 cm high, conical 0.8 x 4 cm End cap

polypropylene columns that hold up to 2 ml of

chromatography resin and 10 ml of eluent or sample in
an integral reservoir. These columns are ideal for sample
10 ml ­reservoir
preparation and small-scale chromatography applications,
including work with radioisotopes and other applications
that require disposable products. Poly-Prep columns may 9 cm

be autoclaved and will retain fine particles.

For More Information
Web: bio-rad.com/polyprepcolumns 3.9 cm working bed height
Download bulletin: 2289
2 ml bed volume

Porous 30 µm polyethylene bed

support retains fine particles

Luer end fitting with snap-off tip

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 141
 Chromatography Columns
Empty Columns bio-rad.com/econocolumns

Ordering Information
Catalog # Description Price, U.S. $
7311550 Poly-Prep Chromatography Columns, empty, includes end caps and tip closures, 50 88.00
7311553 Poly-Prep Chromatography Columns, empty, includes end caps and tip closures, 1,000 1,330.00
Accessories
7311555 Poly-Prep Column Stack Cap, 50 43.00
7317005 Poly Column Rack, 20-place, with removable tube rack 450.00
7328102 2-Way Stopcocks, female-to-male luer, 10 55.00
7318232 Female Luer Plugs, polypropylene, 25 37.00
7311660 End Caps, for disposable plastic columns, 1,000 225.00

Glass Econo-Column Columns

Econo-Column chromatography columns are the standard

Translucent column ends
for high-quality, affordable low-pressure chromatography with male luer lock fittings to
columns. Columns ranging from 5 to 170 cm in length simplify tubing connections
and 0.5–5.0 cm in diameter are available. A porous
polymer bed support at the bottom of the column retains
fine particles, and translucent polypropylene end fittings
allow viewing of the entire column bed. Econo-Column Column reservoir for
manual sample ­application
chromatography columns can be autoclaved and are
designed to operate at pressures <1 bar (14.7 psi).
Econo-Column chromatography columns accept Econo- Accepts accessory
Column funnels as well as Econo-Column flow adaptors. flow adaptors, funnels,
Econo-Column chromatography columns are packaged in and reservoirs

quantities of 1, 2, and 4, depending on column diameter

and length. For convenience, all packaging sizes contain
one stopcock per column ordered.
Borosilicate glass b­ arrel —
clear, inert, durable

Bed support (28 µm)

retains fine ­particles

End caps for s­ topping

eluent flow or storing
packed ­column

For More Information

Web: bio-rad.com/econocolumns
Download bulletin: 2289

142 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/econocolumns Empty Columns

Ordering Information
Cross-Sectional Maximum
Catalog # ID, cm Length, cm Area, cm2 Volume, ml Columns/Pkg Price, U.S. $
7370507 0.5 5 0.20 1 2 57.00
7374506 0.5 5 0.20 1 4 98.00
7374511 0.5 10
DISCONTINUED
0.20 2 4 98.00
7370517 0.5 15 0.20 3 2 57.00
7374521 0.5 20 DISCONTINUED
0.20 4 4 98.00
7370707 0.7 5 0.39 2 2 57.00
7374706 0.7 5 0.39 2 4 98.00
7370712 0.7 10 0.39 4 2 57.00
7374711 0.7 10 0.39 4 4 98.00
7370717 0.7 15 0.39 6 2 57.00
7374716 0.7 15 0.39 6 4 98.00
7374721 0.7 20
DISCONTINUED
0.39 8 4 98.00
7370732 0.7 30 0.39 12 2 57.00
7374731 0.7 30 DISCONTINUED
0.39 12 4 98.00
7370752 0.7 50 0.39 20 2 57.00
7374006 1.0 5 0.79 4 4 98.00
7371007 1.0 5 0.79 4 2 57.00
7374011 1.0 10 0.79 8 4 98.00
7371012 1.0 10 0.79 8 2 57.00
7374021 1.0 20 0.79 16 4 98.00
7371022 1.0 20 0.79 16 2 57.00
7374031 1.0 30 DISCONTINUED
0.79 24 4 98.00
7371032 1.0 30 0.79 24 2 57.00
7374051 1.0 50 DISCONTINUED
0.79 40 4 98.00
7371052 1.0 50 0.79 40 2 57.00
7371091 1.0 100 0.79 79 2 140.00
7371093 1.0 120 0.79 103 2 175.00
7374150 1.5 5 1.77 9 4 150.00
7371507 1.5 5 1.77 9 2 82.00
7374151 1.5 10 1.77 18 4 150.00
7371512 1.5 10 1.77 18 2 82.00
7374156 1.5 15 1.77 27 4 150.00
7371517 1.5 15 1.77 27 2 82.00
7374152 1.5 20 1.77 35 4 150.00
7371522 1.5 20 1.77 35 2 82.00
7374153 1.5 30 1.77 53 4 150.00
7371532 1.5 30 1.77 53 2 82.00
7374155 1.5 50 DISCONTINUED
1.77 89 4 170.00
7371552 1.5 50 1.77 89 2 94.00
7371576 1.5 75 1.77 124 2 125.00
7371591 1.5 100 1.77 177 2 140.00
7371593 1.5 120 1.77 230 2 175.00
7371598 1.5 170 1.77 301 2 250.00
7374250 2.5 5 DISCONTINUED
4.91 25 4 150.00
7372507 2.5 5 4.91 25 2 82.00
7374251 2.5 10 4.91 49 4 150.00
7372512 2.5 10 4.91 49 2 82.00
7374252 2.5 20 4.91 98 4 150.00
7372522 2.5 20 4.91 98 2 82.00
7374253 2.5 30 4.91 147 4 170.00
7372532 2.5 30 4.91 147 2 94.00
7372551 2.5 50 4.91 246 2 125.00
continues

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 143
Discontinued products are no longer available.
 Chromatography Columns
Empty Columns bio-rad.com/econocolumns

Ordering Information
Cross-Sectional Maximum
Catalog # ID, cm Length, cm Area, cm2 Volume, ml Columns/Pkg Price, U.S. $
7372576 2.5 75 4.91 344 2 140.00
7372591 2.5 100 4.91 491 2 195.00
7372593 2.5 120 4.91 589 2 240.00
7375011 5.0 10 19.63 196 1 140.00
7375021 5.0 20 19.63 393 1 175.00
7375031 5.0 30 19.63 589 1 210.00
7375051 5.0 50 19.63 982 1 230.00
7375071 5.0 70 19.63 1,374 1 325.00

Catalog # Description
Econo-Column Selection Packs
7376601 Econo-Column Selection Pack A, includes 7 columns, 1 each of 0.7 x 10, 20, and 30 cm; 275.00
1.5 x 30 and 50 cm; 2.5 x 20 and 50 cm
7376607 Econo-Column Selection Pack B, includes 6 columns, 1 each of 1.0 x 20, 30, and 50 cm; 240.00
1.5 x 20, 30, and 50 cm
For fittings, see low-pressure fittings on page 169.

Econo-Column Flow Adaptors

Flow adaptors significantly improve column performance by Econo-Column flow adaptors are available for 1.0, 1.5, 2.5,
eliminating head space above the gel bed and by protecting and 5.0 cm ID Econo-Column chromatography columns
the column bed from disruption during sample loading. (pages 142–144) and Econo-Pac columns (page 141).
Flow adaptors improve resolution by delivering buffer and Due to wear and tear of bed supports and O-rings,
sample directly to the top of the column bed. Flow adaptors maintenance kits are available for standard upkeep of
are recommended for use with any low-pressure column flow adaptors.
connected to pumps, the BioLogic LP system, or other low-
pressure systems.
3-position cam (1 = loose,
2 = sliding seal, 3 = fixed seal)

Delrin housing (snaps onto Econo-Column

columns or Econo‑Pac columns)

Reliable ethylene-propylene seal

Replaceable porous polymer bed support

For More Information
Replaceable flexible silicone tubing
Web: bio-rad.com/econocolumnaccessories (0.88 mm ID) with replaceable female
Download bulletin: 2289 luer lock fitting

144 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/econocolumns Empty Columns

Ordering Information
Catalog # Description Price, U.S. $
Flow Adaptors
7380014 Flow Adaptor, 1.0 cm column ID, 1–7 cm functional length 140.00
7380015 Flow Adaptor, 1.0 cm column ID, 1–14 cm functional length 140.00
7380016 Flow Adaptor, 1.5 cm column ID, 1–14 cm functional length 140.00
7380017 Flow Adaptor, 2.5 cm column ID, 1–14 cm functional length 140.00
7380018 Flow Adaptor, 5.0 cm column ID, 1–14 cm functional length (does not include cam mechanism) 655.00
7380019 Econo-Pac Flow Adaptor, 1.5 cm column ID 140.00
Flow Adaptor Maintenance Kits
7380022 Flow Adaptor Maintenance Kit, for 5.0 cm flow adaptor, includes 2 bed supports and 2 O-rings 57.00
7380024 Flow Adaptor Maintenance Kit, for 1.0 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings 57.00
7380025 Flow Adaptor Maintenance Kit, for 1.5 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings 57.00
7380027 Flow Adaptor Maintenance Kit, for 2.5 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings 57.00

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 145
 Chromatography Columns
Empty Columns bio-rad.com/econocolumns

Econo-Column Funnel

The Econo-Column funnel, constructed of durable

polypropylene, is ideal for packing columns, loading diluted
samples, or delivering large volumes of buffer. It forms a
tight seal with Econo-Column chromatography columns up
to 2.5 cm ID (pages 142–144), Poly-Prep columns (pages
141–142), and Econo-Pac columns (page 141).
Graduated volume 250 ml Cap with male
markings reservoir luer lock fitting

Ordering Information
Catalog # Description Price, U.S. $
7310003 Econo-Column Funnels, 250 ml, 5 95.00

Glass Econo-Column Reservoirs

Reservoirs are available in 500 ml and 1 L capacities

and will fit 0.5, 0.7, 1.0, or 1.5 cm ID Econo-Column
chromatography columns. To make a constant-pressure
reservoir, close the reservoir top with a stopper that contains
a piece of glass tubing extending into the reservoir. The
Glass Econo-Column Reservoir
removable upper cap has a male luer lock fitting.

Ordering Information
Catalog # Description Price, U.S. $
7379112 Econo-Column Reservoir, 500 ml 210.00
7379113 Econo-Column Reservoir, 1 L 215.00

146 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Chromatography Columns
bio-rad.com/econocolumns Empty Columns

Bio-Scale MT High-Resolution Columns See Also

Bio-Scale CHT Type I
columns: page 136.
Bio-Scale MT empty columns can be packed with the UNO columns:
resin of your choice. These columns provide extremely page 136.
high resolution in most chromatography applications. Bio-Scale Mini
cartridges: page 132.
Bio-Scale MT columns allow precise sample application
NGC systems:
and provide the low dead volume required for high- page 148.
resolution separations. The four column sizes (2, 5, ENrich columns:
10, and 20 ml) allow easy scale-up of separation and page 125.

purification protocols. The optimized design allows easy Adjustable bed support eliminates column voids

packing, bed height adjustment, sample application, Easy assembly for packing and cleaning
and equilibration. Bio-Scale MT columns are convenient ptimized design of sample applicator for
O
for use with BioLogic systems or any medium- or high- Biocompatible
high-resolution chromatography

pressure chromatography system. m ­ aterials for

p ­ reservation of Fingertight assembly
Transparent
For More Information s­ ample biological eliminates the need
glass tube allows
activity for tools
Web: bio-rad.com/MTcolumns easy bed inspection
Download bulletin: 1970

Ordering Information
Catalog # Description Column Volume, ml Pressure Limit, psi Price, U.S. $
Bio-Scale Columns*
7510081 Bio-Scale MT2 Column, 7 x 52 mm 1.9–2.3 1,000 685.00
7510083 Bio-Scale MT5 Column, 10 x 64 mm 4.6–5.7 750 715.00
7510085 Bio-Scale MT10 Column, 12 x 88 mm 9.5–11.3 600 740.00
7510087 Bio-Scale MT20 Column, 15 x 113 mm 19.4–21.9 500 840.00

Catalog # Description Price, U.S. $

Cartridge Holders and Accessories
7510091 Bio-Scale 2 Replacement Parts Kit, includes 5 frits, 5 distribution screens, 2 O-rings, 1 frit remover 125.00
7510093 Bio-Scale 5 Replacement Parts Kit 125.00
7510095 Bio-Scale 10 Replacement Parts Kit 125.00
7510097 Bio-Scale 20 Replacement Parts Kit 125.00
7500554 1/16" OD (1.6 mm) Post-Pump Fittings, includes Delrin nut, ferrules, lock ring, 10 sets 185.00
7500567 UNO M6 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an FPLC system 36.00
7500568 UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect UNO column to an HPLC system, for use with 47.00
BioLogic DuoFlow and NGC systems

* Each Bio-Scale MT column comes with a Bio-Scale replacement parts kit.

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 Medium-Pressure Chromatography Systems
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Medium-Pressure Chromatography Systems

See Also NGC Medium-Pressure Chromatography Systems

Multi-D
chromatography
applications: The NGC family of medium pressure preparative chromatography systems offers a single laboratory chromatography
page 150.
solution that aligns and scales to fit your purification, automation, and throughput requirements. NGC laboratory scale
chromatography systems provide you with a fully customizable and truly modular platform that can be designed to your
current purification needs and is upgradable to meet future throughput and automation requirements.
For More Information
Web: bio-rad.com/NGCsystems
Please contact your local Sales Representative for more details.

Learn More about the Technology

Web: bio-rad.com/tech/chrom

NGC Discover Series

NGC Quest and NGC Scout Series NGC Discover Pro Series

NGC chromatography system platforms — modular, customizable, and upgradable systems that adapt and expand to your throughput
and application needs. All NGC systems are compatible with the NGC fraction collector and BioFrac fraction collector.

NGC Fraction Collector BioFrac Fraction Collector

(see page 161) (see page 163)

148 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Medium-Pressure Chromatography Systems
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NGC Chromatography Systems Selection Guide

NGC Discover Pro 100

NGC Discover Pro 10
NGC Quest 100 Plus

NGC Scout 100 Plus

NGC Quest 10 Plus

NGC Scout 10 Plus

NGC Discover 100

NGC Discover 10
NGC Quest 100

NGC Scout 100

NGC Quest 10

NGC Scout 10

7880006

7880009
7880008
7880005
7880003

7880004
7880002
7880001

7880007

7880010

7880012
7880011
Product Catalog #
NGC F10 pump module 7884002 • • • • • •
NGC F100 pump module 7884003 • • • • • •

DISCONTINUED

DISCONTINUED

DISCONTINUED

DISCONTINUED
NGC mixer module 7884018 • • • • • • • • • • • •
NGC sample inject valve module 7884007 • • • • • • • • • • • •
DISCONTINUED
NGC single-wavelength detector module 7884008 • • • •
NGC multi-wavelength detector module II 12010343 • • • • • • • •
NGC buffer blending valve module 7884010 • • • • • • • •
NGC pH valve module (includes pH probe) 7884011 • • • • • • • •
NGC sample pump module 7884004 • • • •
NGC inlet valve module 7884006 • • • •

NGC outlet valve module 7884013 • •
NGC column switching valve module, 10 ml 7884012 • •
NGC column switching valve module, 100 ml 7884026 • • • •

ChromLab software 7886000 • • • • • • • • • • • •
NGC chromatography system and
7886500 • • • • • • • • • • • •
ChromLab software documentation
Note: All NGC systems include a touch screen and conductivity monitor and are compatible with the NGC fraction collector and BioFrac
fraction collector.

Features and Benefits

Flexible, customizable chromatography systems to suit ■
 ier-Rotate system design enables optimal placement of
T
both your application and research needs. valves and detectors to minimize hold-up volume
■
 djustable and scalable platform adapts to individual
A
■
 pen platform is compatible with all medium-pressure
O
application, workflow, and throughput requirements chromatography columns and resins
■
 lug-and-play modules enable a modular, customizable
P
■
 ompatibility of NGC and BioFrac fraction collectors
C
system that adapts to changing needs over time allows for collection from analytical to preparative
scale purifications
■
 hromLab software provides powerful, graphical
C
instrument control, streamlined method development,
■
 ompact footprint fits on lab bench, in deli-fridge,
C
and intuitive data analysis or in coldroom
■
 raphical fluidics scheme selector matches the flow
G For more information about these features and benefits,
path to applications-based system setup please contact your local sales representative. Take a tour
■
 oint-to-Plumb feature lighting provides step-by-step
P of the system at bio-rad.com/NGCsystems.
LED guided setup for easy system plumbing Applications and Uses of Medium-Pressure
■
 eal-time active flow path display for clear visualization
R Chromatography Systems
of buffer, sample, and valve position and easy Preparative to analytical scale isolation, purification,
identification of system status and analysis of multiple types of molecules, including:
■
 eal-time module status displays offer immediate
R ■
Recombinant protein purification and refolding
system diagnostics ■
Monoclonal antibody purification
■
 re-plumbed systems with QC-validated performance
P ■
Virus removal preparations
support more reproducible results and sharper peaks ■
Analysis of plasma proteins for disease diagnosis
(included with all preconfigured NGC Quest, NGC Scout,
and NGC Discover systems) ■
Nucleic acid purifications

In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 149
 Medium-Pressure Chromatography Systems
bio-rad.com/NGCsystems

NGC Systems
Preconfigured NGC systems are designed with increasing automation and throughput in mind. All NGC systems include
either a 10 or a 100 ml/min automated dual gradient pump, a mixer module, a sample injection valve module, and a
detector with a conductivity monitor. Each preconfigured system can be further customized with additional modules to
meet your specific purification needs.

NGC Quest and NGC Quest Plus NGC Discover and Discover Pro
NGC Quest systems are designed for the all-purpose Both the NGC Discover and NGC Discover Pro
purification of proteins with automated pumps that chromatography systems are designed for higher
provide accurate gradients for high-resolution separations. throughput applications with rapid, robust automation
These systems support automated sample injection using for those who need to do method development.
fixed or dynamic sample loops.
NGC Discover 10 and 100 systems include the capabilities
NGC Quest 10 and Quest 100 systems feature: of the NGC Scout 10 and 100 Plus instruments but also
■
 ED-based single-wavelength light source for the
L comprise:
detection of proteins or nucleic acids (280 or 255 nm) ■
 100 ml/min integrated sample pump — supports
A
with high-sensitivity conductivity measurements for contamination-free automated large-volume
accurate salt gradient formation sample application
NGC Quest 10 Plus and Quest 100 Plus systems feature:
■
Inlet valves — enable automated switching between
buffers and samples, accelerating method development.
■
 ulti-wavelength (4) detector for simultaneous detection
M
NGC supports up to 2 inlet valves for buffers and
(190 to 800 nm) of proteins, peptides, nucleic acids,
2 inlet valves for samples
chromophores, and other biomolecular complexes with
high-sensitivity conductivity measurements for accurate
■
 column switching valve — facilitates automated
A
salt gradient formation column/resin scouting up to 5 columns without the
need to replumb. NGC supports up to 3 column
NGC Scout and NGC Scout Plus switching valves
NGC Scout systems are designed for method optimization ■
 sample outlet valve, a standard feature with the
A
with automated pumps and valves that provide accurate
NGC Discover Pro systems — allows collection of up
gradients and pH for high-resolution separations suitable
to 12 large-volume fractions and enables automated
for any application.
Multi-D chromatography applications. NGC supports
All NGC Scout 10 and Scout 100 systems include: up to 2 outlet valves
■
All features of NGC Quest systems Learn More about Multi-D Chromatography
Web: bio-rad.com/Multi-D
■
 uffer blending to automate buffer titrations for rapid
B
scouting of pH parameters
■
 ompatibility for gradient separations at different pH
C
values for rapid method development
The NGC Scout 10 and 100 systems feature:
■
 ingle-wavelength UV (255 or 280) and conductivity
S
detector
NGC Scout 10 Plus and Scout 100 Plus systems feature:
■
Multi-wavelength (4) UV/Vis and conductivity detector

150 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Medium-Pressure Chromatography Systems
bio-rad.com/NGCmodules

Video
Ordering Information
Catalog # Description Price, U.S. $ Product
Demonstration
NGC Medium-Pressure Chromatography Systems*
7880001 NGC Quest 10 Chromatography System, includes automated 10 ml/min pumps, single-wavelength Inquire
DISCONTINUED
(UV) and conductivity detection, accurate gradients, and sample injection
7880003 NGC Quest 10 Plus Chromatography System, includes automated 10 ml/min pumps, multi-wavelength Inquire
(UV/Vis) and conductivity detection, accurate gradients, and sample injection
7880002 NGC Quest 100 Chromatography System, includes automated 100 ml/min pumps, single-wavelength (UV) Inquire bio-rad.com/
DISCONTINUED
and conductivity detection, accurate gradients, and sample injection NGCMulti-D
7880004 NGC Quest 100 Plus Chromatography System, includes automated 100 ml/min pumps, multi-wavelength Inquire
(UV/Vis) and conductivity detection, accurate gradients, and sample injection
7880005 NGC Scout 10 Chromatography System, includes NGC Quest 10 capability, automated 10 ml/min Inquire
pumps, pH valve and buffer blending valveDISCONTINUED
for automated gradient and buffer blending
7880007 NGC Scout 10 Plus Chromatography System, includes NGC Quest 10 Plus capability, automated 10 ml/min Inquire
pumps, pH valve and buffer blending valve for automated gradient and buffer blending
7880006 NGC Scout 100 Chromatography System, includes NGC Quest 100 capability, automated 100 ml/min Inquire
pumps, pH valve and buffer blending valveDISCONTINUED
for automated gradient and buffer blending
7880008 NGC Scout 100 Plus Chromatography System, includes NGC Quest 100 Plus capability, automated Inquire
100 ml/min pumps, pH valve and buffer blending valve for automated gradient and buffer blending
7880009 NGC Discover 10 Chromatography System, includes NGC Scout 10 Plus capability, automated 10 ml/min Inquire
pumps, sample pump for large sample injection, column switching valve (up to 5 columns), and buffer inlet valve
7880010 NGC Discover 100 Chromatography System, includes NGC Scout 100 Plus capability, automated 100 ml/min Inquire
pumps, sample pump for large sample injection, column switching valve (up to 5 columns), and buffer inlet valve
7880011 NGC Discover Pro 10 Chromatography System, includes NGC Scout 10 Plus capability, automated 10 ml/min Inquire
pumps, sample pump for large sample injection, sample inlet valve, column switching valve (up to 5 columns),
2 buffer inlet valves, and outlet valve
7880012 NGC Discover Pro 100 Chromatography System, includes NGC Scout 100 Plus capability, automated 100 ml/ Inquire
min pumps, sample pump for large sample injection, sample inlet valve, column switching valve (up to 5 columns),
2 buffer inlet valves, and outlet valve
* All NGC systems include ChromLab software.

Multi-D Chromatography
12001088 NGC Multi-D Chromatography Starter Kit Inquire

Module Overview
The NGC family of medium-pressure chromatography systems offers flexible system configurations that enable upgrades
and reconfiguration of the modules to fit multi-user needs, applications, and laboratory space requirements. All NGC
systems can be individually customized using NGC plug-and-play modules that are user installable. Systems can be easily
reconfigured and seamlessly upgraded with increased functionality such as higher flow rates (10 ml/min or 100 ml/min),
sophisticated detection capabilities, pH monitoring, automated buffer selection, multicolumn connection, and buffer blending.

NGC Buffer Blending Valve Module

The NGC buffer blending valve is used for automatic
online buffer preparation and generation of pH gradients
NGC Buffer Blending Valve Module
for quick pH scouting. When used in conjunction with
the dual gradient pump system, the buffer blending
valve enables automated elution gradients and highly
accurate buffer blending for rapid scouting and method
development. The valve can also double the flow rate of a
salt gradient on an F10 pump and F100 pump.
NGC Inlet Valve Module
The NGC inlet valve module allows for automatic buffer
and sample selection during method running. It speeds
up method development by allowing for automatic selection
of up to seven buffers and a cleaning solution per valve.
NGC Inlet Valve Module
It also works as a mini autosampler for injecting multiple
large-volume samples via the sample pump.

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 Medium-Pressure Chromatography Systems
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NGC F10 and F100 System Pump Modules The outlet valve also enables automated tandem/2-D and
The F10 and F100 pumps produce buffer gradient Multi-D chromatography applications by directing peaks
solutions. Flow rates on all pumps can be automatically of interest onto the next column.
controlled to avoid overpressure. NGC instruments can
have up to three high-precision pumps: two system
gradient pumps (Pump A and Pump B) and one
sample pump.
NGC Sample Pump 100 Module
The NGC sample pump 100 module enables both the
injection of large sample volumes and accurate and
consistent multiple loadings from a single sample stock. NGC Sample Pump
This dedicated pump eliminates the risk of contaminating Module
system pumps, allowing for automated loading of large
sample volumes directly to the column. The sample pump
also includes an integrated pressure sensor that protects
the column and resin from overpressure. Specifications for System and Sample Pump Modules
F10 Pump
NGC Sample Inject Valve Module
Flow rates 0.001–10 ml/min (normal range);
The NGC sample inject valve enables the system to load
0.002–20 ml/min (column packing flow)
a specific predetermined volume of sample onto a column
Max pressure limit 3,650 psi (252 bar, 25.2 MPa)
with a maximum operating pressure of 3,650 psi. It is
also compatible with capillary loops and sample pumps, F100 Pump
eliminating the need to replumb. Flow rate setting 0.01–100 ml/min (normal range);
0.02–200 ml/min (column packing flow)
NGC Multi-Wavelength Detector Modules Max pressure limit 1,450 psi (100 bar, 10 MPa)
The NGC UV/Vis detector module is combined with Sample Pump 100
an integrated conductivity monitor to measure buffer Flow rate Up to 100 ml/min
conductivity or salt gradients. Max pressure limit 1,450 psi (100 bar, 10 MPa)
The multi-wavelength UV/Vis detector adds flexibility to
the chromatography system, enabling the simultaneous
monitoring of four wavelengths (UV and Vis) for greater
sensitivity and detection of proteins, peptides, nucleic
acids, and chromophores such as hemoglobin.
NGC Outlet Valve Module
Use the NGC outlet valve either as a stand-alone unit
or in conjunction with the BioFrac fraction collector
(#7410002).
NGC Multi-Wavelength
As a single, stand-alone unit, the outlet valve is capable Detector Module
of collecting up to 12 large-volume fractions such as
flowthrough or waste. Two outlet valves may be daisy-
chained to collect up to 23 large-volume fractions,
including waste.
When a BioFrac fraction collector is connected, the Specifications for Single-/Multi-Wavelength Detector Modules
collection sequence can be programmed to collect UV Monitor
smaller-volume fractions in the BioFrac fraction collector Single-wavelength range 255 or 280 nm
and larger-volume fractions via the outlet valves, providing Multi-wavelength (up to 4) range 190–800 nm
full flexibility. Conductivity Monitor
Conductivity range 0.01–999.99 mS/cm

152 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
 Medium-Pressure Chromatography Systems
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Features and benefits of the NGC outlet valve

module include:
■
Collect up to 12 large-volume fractions with a single valve
■
Collect up to 23 large-volume fractions with two valves
■
 ollect variable fraction sizes with the BioFrac
C
fraction collector
■
Automate multistep purifications
NGC pH Valve Module
The NGC pH valve module includes a pH electrode
for accurate pH monitoring during the run (1–14). NGC pH Valve Module
The valve is capable of directing flow to the pH electrode
or bypassing the probe automatically. The valve includes
an integrated calibration port, which can be used to
calibrate the probe without disconnecting the probe
from the system.
For sensitive applications, the software is capable of
calculating and displaying temperature-compensated pH
values for accuracy and ease of use.
NGC Mixer Module
The NGC mixer module homogenizes buffers from two
system pumps (Pumps A and B). In addition to the mixer
motor assembly, the module includes an integrated system
pressure sensor. It can accommodate both F10 mixers NGC Air Sensor Module
(263 µl and 750 µl) and F100 mixers (2, 5, and 12 ml).
The mixer volume can be adjusted, depending on flow
rate, to achieve optimal buffer homogenization by inserting
the appropriate size barrels.
NGC Air Sensor Module Signal Import Module
The NGC air sensor module enables detection of the Adding external devices to a purification workflow provides
end of buffer and sample, thereby protecting against air the flexibility to increase automation and the amount of
entering the system and damaging pumps or columns. information collected. The NGC signal import module (SIM)
The module supports up to four air sensors (large- and enables analog to digital signal conversion and connection
small-bore). Each NGC air sensor extension module can to third-party devices such as external detectors or
be used in conjunction with the air sensor module to autosamplers.
enable four additional air sensors (totaling up to eight).
NGC Accessories
NGC Column Switching Valve Module
The NGC column switching valve allows connection of up NGC Fraction Collector
to five columns, enabling quick and easy column scouting The NGC fraction collector’s flexibility and versatility
without replumbing. With an internal bypass mode this improves high-throughput purification workflows. It can
valve lets buffers bypass the connected columns when collect into multiple vessel types within a method and has
priming or cleaning the system. The valve can also increased capacity in comparison to the BioFrac fraction
reverse the flow, which is ideal for column cleaning and collector. The NGC chromatography system paired with
applications that require narrow band elution. the NGC fraction collector provides collection options for
analytical to preparative chromatography applications and
The column switching valve has integrated pressure is well suited to meet the purification needs of industry
sensors that measure pre- and delta-column pressures. laboratories. Please refer to page 161 for detailed
This protects the column and resin from overpressure by product information.
triggering the pumps to slow down or stop. The column
switching valve can also be used to connect sample loops BioFrac Fraction Collector
in multistep purifications. The BioFrac fraction collector is compatible with all
NGC chromatography systems. It is ideal for analytical
to preparative scale chromatography applications.
Please refer to page 163 for detailed product information.

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 Medium-Pressure Chromatography Systems
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Ordering Information
Catalog # Description Price, U.S. $
NGC Medium-Pressure Chromatography System Modules and Accessories
System Pumps
7884002  GC F10 Pump Module, includes 10 ml/min system pump kit for creating buffer gradients; can be used
N 5,450.00
in conjunction with buffer blending valve to generate flow rates up to 20 ml/min; kit includes necessary
tubing and fittings
7884003 NGC F100 Pump Module, includes 100 ml/min system pump kit for creating buffer gradients; can 7,500.00
be used in conjunction with buffer blending valve to generate flow rates up to 200 ml/min; kit includes
necessary tubing and fittings
Sample Pump
7884004  GC Sample Pump 100 Module, includes 100 ml/min sample pump kit for automated large-volume sample
N 5,950.00
application via the sample inject valve; kit includes necessary tubing and fittings
Detectors
7884008  GC Single-Wavelength Detector Module, for detection of nucleotides and proteins at 255 or 280 nm and
N 4,950.00
generation of salt gradients; UV/conductivity detector kit includes necessary tubing and fittings
7884009 NGC Multi-Wavelength Detector Module, DISCONTINUED
for simultaneous wavelength (4) monitoring of elution fractions Inquire
190–800 nm and generation of salt gradients; UV/Vis, conductivity detector kit includes necessary tubing
and fittings
7885024 NGC UV Flow Cell, 5 mm, UV flow cell, standard with all systems. Fits single-wavelength UV and 580.00
multi-wavelength UV/Vis detectors in both F10 and F100 systems
7885023 NGC UV Flow Cell, 10 mm, analytical UV flow cell. Fits single-wavelength UV and multi-wavelength UV/Vis 580.00
detectors in F10 systems. Ideal for use at lower flow rates
7885022 NGC UV Flow Cell, 2 mm, preparative UV flow cell. Fits single-wavelength UV and multi-wavelength UV/Vis 1,450.00
detectors in F100 systems. Ideal for use at high flow rates
7500230 Backpressure Regulator, 40 psi, restricts flow and creates appropriate backpressure to prevent bubble 160.00
spiking in UV detectors
Detector Lamp Replacements
7885000 NGC LED Lamp Replacement, includes 255/280 nm LED lamp replacement for single-wavelength detectors 1,260.00
7885001 NGC Deuterium Lamp Replacement, includes deuterium lamp replacement (UV) for multi-wavelength detectors 2,075.00
7885002 NGC Tungsten Lamp Replacement, includes tungsten lamp replacement (Vis) for multi-wavelength detectors 630.00
7885056 NGC Replacement Conductivity Monitor, includes replacement conductivity monitor Inquire
pH Valve
7884011 NGC pH Valve Module, for accurate inline pH measurement; includes pH probe, tubing, and fittings 4,100.00
7885026  GC pH Probe (Ag/AgCl), for use with the pH valve for accurate pH monitoring (pH 0–14) during purifications
N 500.00
7885027 NGC Blank pH Probe, pH valve cap for use with the pH valve when the probe is removed Inquire
Mixer
7884018  GC Mixer Module, for use with all NGC systems; additional mixer base or barrels (ordered separately) can be
N 2,100.00
extended with 2, 5, and 12 ml barrels for efficient gradient mixing at higher flow rates
7884019 NGC F100 Mixer, 750 µl base and top assembly, included with all 100 ml/min NGC systems 625.00
7884020 NGC F10 Mixer, 263 µl base and top assembly, included with all 10 ml/min NGC systems 210.00
7884021 NGC F10 Mixer Barrel Kit, 750 µl extension barrel for F10 263 µl mixer (part of Scout 10 and Discover 10 series) 99.00
7884022 NGC F10 Mixer Barrel Kit, 2 ml extension barrel for F10 263 µl mixer (optional) 99.00
7884028 NGC F100 Mixer Barrel Kit, 2 ml extension barrel for F100 750 µl mixer body, part of Scout 100, Discover 100 series 250.00
7884023 NGC F100 Mixer Barrel Kit, 5 ml extension barrel for F100 750 µl mixer (optional) 250.00
7884024 NGC F100 Mixer Barrel Kit, 12 ml extension barrel for F100 750 µl mixer (optional) 450.00
Valves
7884006  GC Inlet Valve Module, for automated switching between multiple buffers and samples during methods, kit
N 3,200.00
includes the necessary tubing and fittings
7884007 NGC Sample Inject Valve Module, for manual sample application of small-volume samples via sample loops or 2,600.00
large volume samples using a sample pump; kit includes necessary tubing, fittings, and sample injection port;
valve can also be used with DynaLoops and sample pumps
7884010 NGC Buffer Blending Valve Module, for online buffer preparation and generating pH gradients for quick pH 5,500.00
scouting; kit includes the necessary tubing and fittings; can also double system pump flow rate
7884012 NGC Column Switching Valve Module, 10 ml, for use with F10 systems and multiple columns for quick column 5,500.00
scouting and reverse flow, holds 5 columns; kit includes the necessary tubing and fittings to accommodate the most
common column types
7884013 NGC Outlet Valve Module, for collection of up to 12 large-volume fractions, including necessary tubing and fittings 3,000.00
7884026  GC Column Switching Valve Module, 100 ml, for use with F100 systems and multiple columns for quick column
N 5,500.00
scouting and reverse flow, holds 5 columns; kit includes the necessary tubing and fittings to accommodate the most
common column types
continues

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Ordering Information
Catalog # Description Price, U.S. $
Maintenance Kits
7885003 Pump Maintenance Kit, 10 ml, for regular maintenance of NGC F10 pumps, includes seals and check valves 950.00
7885004 Pump Maintenance Kit, 100 ml, for regular maintenance of NGC F100 pumps, includes seals and check valves 1,070.00
7885005 Pump Maintenance Kit, Sample pump, 100 ml, for regular maintenance of NGC sample pump, 3,375.00
includes seals and check valves
Air Sensor
7885017 NGC Air Sensor Module, for detection of end of buffer and sample, protects against air entering pumps and 3,200.00
columns, supports up to 4 air sensors (large- and small-bore); kit includes 2 large-bore air sensors
7885018 NGC Air Sensor Extension Module, connects to the base air sensor module to support 4 additional air sensors 820.00
(optional, does not include any air sensors)
7885020 NGC Air Sensor, small, includes air sensor; enables exclusion of air from system and columns. 1,600.00
Detects air in small-diameter PEEK tubing
7885021 NGC Air Sensor, large, includes air sensor; enables exclusion of air from system and columns. 1,600.00
Detects air in large-diameter PTFE tubing
Fraction Collectors
17002070 NGC Fraction Collector, 100/240 V, includes two 13 mm racks, tubing kit, external power supply, 5,415.00
and USB cable
7410002 BioFrac Fraction Collector, 100/240 V, fraction collector compatible with all NGC systems, includes 5,415.00
power cord, rack set F1 (2 x flatpack, 13 mm), BioFrac diverter valve, fittings kit
7884025 NGC Communication Adaptor, enables communication between Bio-Rad devices such as the BioFrac 395.00
fraction collector and the NGC system
7884016 NGC Signal Import Module, enables analog to digital signal conversion and connection to devices 1,500.00
such as third-party autosamplers and external detectors, includes cable for connecting to NGC system
(#7885013) and external detectors
NGC Accessories
7885038 NGC Column Holder, column holder for use with NGC systems, pkg of 1 150.00
7885039 NGC Cartridge Holder, universal cartridge holder, holds 1–10 ml cartridges 68.00
7885041 NGC Sample/Wash Tube Holder, holds two 50 ml Falcon tubes, for use with NGC systems (tubes not included) 68.00
78850