ZECN-8034

Apr. 2019

DGU-403/DGU-405

Service Manual
This manual is for professional service personnel.
Do NOT use this manual unless you have received proper training.
This page is intentionally left blank.
Contents
1 Introduction 1.1 1.1 Revision history ............................................. 1

2 Safety Precautions 2.1 Warning Labels ................................................... 2

3 Specifications 3.1 Specifications ...................................................... 3

3.1.1 DGU-403/DGU-405 Specifications ............................. 3
3.1.2 Other Specifications ............................................. 3

3.2 Principle of Operation ......................................... 4

3.2.1 制御方式 ........................................................ 4
3.3 Safety Measures .................................................. 5
3.3.1 Installing Tubes and Joints for Draining Leakage .............. 5

3.3.2 Residual Risk Information ....................................... 7

4 Installation 4.1 Installing DGU-403／DGU-405 ............................... 9

4.1.1 Removing the Shipping Screw .................................. 9

4.1.2 Connecting to the Pump ....................................... 9

Instrument Components 11
5 and Parts Lists
5.1 Inside the device ...............................................

5.2 Parts compatible table DGU-40 and DGU-20AR .... 12

Dismantling and 14
6 assembling each unit
6.1 Periodic Checks and Maintenance .......................

6.1.1 Preparation for Periodic Checks and Maintenance ........... 14

6.1.2 List of Periodic Checks and Maintenance .................... 14

6.1.3 Precautions when not using for a long time ................ 15

6.2 Parts Replacement ............................................. 16

6.2.1 Removing the Cover .......................................... 16

DGU-403/DGU-405 i
 Contents

6.2.2 Replacing the Vacuum Pump (228-63035) ................... 17

6.2.3 Replacing the Degassing Chamber (228-54502) ............. 18

6.2.4 Replacing the PCB (228-70250) .............................. 19
6.2.5 Replacing theLED PCB (228-70251) .......................... 21
6.2.6 Replacing the Filters (228-54509-02) on the Vacuum
Pump .......................................................... 23
6.2.7 Replacing the Vacuum Tube Filter (228-54656) ............. 23

Maintenance and 25
7 Calibration
7.1 Screens of Solvent Delivery Module ....................

7.1.1 Monitoring Screens ............................................ 25

7.1.2 VP Function Screens / Maintenance Information Group ..... 25

Troubleshooting and 27
8 Corrective Action
8.1 Troubleshooting ................................................

8.1.1 Test Points on Control Board ................................ 27

8.1.2 Precautions for Solvents ....................................... 28

9 Wiring Diagram

10 Maintenance Parts

ii DGU-403/DGU-405
1 Introduction
n Scope of Service Manual 1
This service manual is intended for field engineers certified by Shimadzu and describes
maintenance procedures for DGU-403/405 degassing unit used in Shimadzu liquid
chromatographs. Actual maintenance work should only be performed by a field engineer
that has received training for that instrument. There are places where material covered
by the Instruction Manual has been omitted. Therefore, use the Instruction Manual in
conjunction with this manual, as necessary. New information required for maintenance
will be issued whenever necessary in a Technical Information bulletin. Insert such
Technical Information bulletins into this service manual and use them in conjunction with
this manual.

n Notice

Due to product improvements, some of the parts or figures indicated in the service
manual may differ from the actual instrument. In addition, please understand that we
do not handle all of the parts described in the text as repair parts.

1.1 1.1 Revision history

Serial
Headline Page Changes
number
Initial relea se：2019/03/22

DGU-403/DGU-405 1
 2 Safety Precautions
To ensure maintenance and adjustment work is performed correctly, read these safety
precautions carefully before performing any maintenance or adjustments to the product.
Always observe all precautions indicated below, which provide important safety
information.

Indication Meaning

Indicates a potentially hazardous situation which, if not

avoided, could result in serious injury or possibly death.

Indicates a potentially hazardous situation which, if not

avoided, may result in minor to moderate injury or equipment
damage.
Emphasizes additional information that is provided to ensure
the proper use of this product.

The following symbols are used in this manual:

Indication Meaning

Indicates an action that must not be performed.

Indicates an action that must be performed.

Indicates information provided to improve product performance.

Indicates the location of related reference information.

2.1 Warning Labels

In order to ensure safety, warning labels are attached in places requiring caution. If a
warning label is lost or damaged, obtain a new label through your Shimadzu
representative and attach it in the correct position.
Although there is no warning label on the degassing unit, perform maintenance carefully.

2 DGU-403/DGU-405
3 Specifications
3.1 Specifications

3.1.1 DGU-403/DGU-405 Specifications

Item Specification
• DGU-403: 3 channels
Number of Channels
• DGU-405: 5 channels
When a UV absorption detector with wavelength of 210 nm and
sensitivity of 0.5 AU is used and methanol saturated with dissolved
Degassing
oxygen (HPLC grade at 25 °C) is flowed at a rate of 1 mL/min, UV
Performance
absorption difference with or without the degassing unit is 300 mAU 3
or more
10 mL/min per channel
• Assuming that the degassing capacity of He degassing of methanol
Maximum Operating as 100 %, the capacity at the flow rate of 1 mL/min and 10 mL/min
will be 89 % and 25 % respectively.
Flow Rate
• If more degassing capacity than the above is required, ultrasonic
vacuum degassing of the mobile phase etc. shall be carried out
beforehand.
Internal Capacity 400 µL/1 channel
±0.1 MPa (only for suction with the solvent delivery module. It is
Pressure Resistance
not possible to pressurize the degassing unit.)
A mechanism is provided that operates stably by capturing outside
Self Purge
air during vacuum pump operation

If the maximum operating flow rate is exceeded, bubbles may appear or the
degassing chamber may be overloaded and damaged.

3.1.2 Other Specifications

Item Specification
Liquid-Contacting
Fluororesin, PEEK, FEP, SUS316L, PPS
Part Materials
Operating
Temperature 4 to 35 °C
Range
Humidity Range 20 ％ to 85 ％ (non condensing environment)
Available pH Range 1 to 14
PTFE tube having external diameter of 3 mm ´ internal diameter of
Piping Used
1.5 mm
Dimensions W260 × H72 × D500 mm (Excluding protruding parts)

DGU-403/DGU-405 3
 3 Specifications

Item Specification
• DGU-403：4 kg
Mass
• DGU-405：4 kg
• Ratings of contacts: 5 V、10 mV
• Vacuum pressure monitor:

Voltage Vacuum Pressure Output

External Output
2 V 0 kPa (atmospheric pressure)
0.18 V -90.6 kPa
0.1 V -94.6 kPa

24 V DC, SELV (Power is supplied by connecting a cable to the

Power Supply
solvent delivery module)
Installation Installation Category Ⅱ, Pollution Degree 2, Altitude 2000 m
Environment (IEC) Installed indoor.

3.2 Principle of Operation

3.2.1 制御方式

After turning ON, the vacuum pump is vacuuming in the inner flow line, lit the green
LED after the vacuum level reach to threshold (11kPa, displayed -90.6kPa on PUMP

4 DGU-403/DGU-405
 3.3 Safety Measures

screen). This time is about several second. Normally, vacuum pressure is controlled
about -95kPa after this.
After turning ON, when vacuum level dose not reach the threshold, error happen after
40 min. Once the vacuum pressure reaches to the control pressure, if the degree of
vacuum is kept lower than the threshold for 6 min, the error LED illuminates and the
degassing unit will be turned off.

3.3 Safety Measures

3.3.1 Installing Tubes and Joints for Draining Leakage

This instrument is designed so that if leaks ccur internally, the leaked liquid flows down
to the lowest level of the instrument and is drained into the waste container. However, 3
the column oven is designed so that leakage from instruments above it does not flow
down. Select and install an appropriate drain joint depending on the environment in
which the instrument is installed.

Be careful to prevent drain tubes from bending over and flattening the
internal diameter.
That could prevent the liquid waste from draining and cause the liquid to flow
into the instrument.

Make sure the horizontal portions of liquid waste tubes on the installation
base no longer that 200 mm.
If the horizontal portions of liquid waste tubes on the installation base are
longer that 200 mm, it could make it difficult for liquid waste to drain
properly, which could result in liquid waste flowing into the instrument.

Do NOT leave any organic solvents in the liquid waste bottle where the
column oven drain tube is inserted.
Organic solvent remaining in the liquid waste bottle could trigger the column
oven gas sensor.

• To improve liquid waste flow, orient the end of the silicone tubing downward.
• Cut the silicone tubing to a length that prevents sagging.

DGU-403/DGU-405 5
 3 Specifications

Units Installed Second from the Bottom or Higher

1 From the front side, insert a drain

adapter (1) into the leakage drain port.

2 Rotate the drain adapter

counterclockwise 45 degrees.
That secures the drain adapter.

3 Place the instrument in the position

where it will be used.

No. Name
1 Upper level instrument
2 Leakage hole
3 Drain adapter
4 Leakage port
5 Lower level instrument

A drain adapter is used to connect the

leakage drain port to the leakage hole a
lower level instrument.

Placing an Instrument on Top of a Column Oven (CTO-40C)

Column ovens are designed so that leakage from instruments above does not flow down.

1 From the front side, insert the included

CTO drain outlet tube (1) into the
leakage drain port.

2 Rotate the CTO drain outlet tube

counterclockwise 45 degrees.
That secures the CTO drain outlet tube.

6 DGU-403/DGU-405
 3.3 Safety Measures

3 Use the included straight joint (1) to

connect the included silicone tube (2)
to the CTO drain outlet tube.

4 Insert the opposite end of the silicone

tube into the liquid waste bottle.

5 Pour some water near the leakage

drain port of the uppermost
instrument to verify that the water drains into the liquid waste bottle.

3.3.2 Residual Risk Information 3

A residual risk indicates a risk that could not be reduced or eliminated in the process of
design and manufacture. Check the risk locations in "Residual Risk Map", and take the
relevant protective measures described in "List of Residual Risks".

n Residual Risk Map

The "Mechanical Location" and "No." indicated below are in accordance with those in
"List of Residual Risks". For details, see "List of Residual Risks".

n List of Residual Risks

The "No." and "Mechanical Location" indicated below are in accordance with those in
"Residual Risk Map". Be sure to check the actual "Mechanical Location" referring to
"Residual Risk Map".

DGU-403/DGU-405 7
 3 Specifications

Furthermore, read through and understand the content in "Reference" to take

appropriate protective measures.

8 DGU-403/DGU-405
4 Installation
4.1 Installing DGU-403／DGU-405

• Place mobile phase bottles in the reservoir tray and place the tray on the top
level of the system.
If the mobile phase bottle is placed below the degassing unit, air bubbles can
sometimes form from air that travels through the mobile phase tubing due to
negative pressure that results within the degassing membrane when the system
power is switched OFF.
If placing mobile phase bottles lower than the degassing unit is unavoidable,
then purge the mobile phase thoroughly to eliminate air bubbles before use.

4.1.1 Removing the Shipping Screw

A shipping screw is attached to the instrument for preventing shock during

transportation. Remove the shipping screw before installation.
4
1 Remove the transportation screw (1 place).

If the instrument is used with the shipping screw attached, vibrations of the
instrument may be transmitted across the system resulting in abnormal noise on
the baselines.

4.1.2 Connecting to the Pump

Power is provided to the degassing unit when the power switch for the solvent deliverly
module is turned on. The power supply voltage is 24 volts DC.

If the fastening screws on the power cord are difficult to tighten, use a regular screwdriver.

DGU-403/DGU-405 9
 4 Installation

1 Use the power cord (1) included with

the degassing unit to connect the
solvent delivery pump connector
[DGU/SV] (3) and the degassing unit
[PUMP] power supply connector (2).

2 Use the pressure signal cable (B)

included with the degassing unit to
connect the solvent delivery pump
connector [DGU PRESS IN] (c) and the
degassing unit [DGU PRESS OUT]
connector (d).
When a pressure signal cable is connected,
the vacuum level in the degassing unit’s
internal flow channels is displayed on the
solvent delivery unit monitor.
For display instructions, refer to the
solvent delivery unit instruction manual.

n Connecting the Ground Cable

External noise can cause a red LED

indicator to illuminate when the external
output terminal (ALARM terminal) is
connected or cause the vacuum level to
fluctuate when the [DGU PRESS OUT]
connector (vacuum level output) is
connected. If so, connect a ground cable
to the pump or detector as shown.
Only loosen the screw enough to insert the
crimped terminal. The screw can be
difficult to screw back in if completely
removed.

No. Label or Name

1 GND Cable
2 Crimped Terminal (on DGU unit)
3 Main Unit Ground Terminal
4 Screw
5 Crimped Terminal (on pump/detector unit)

10 DGU-403/DGU-405
5 Instrument Components and Parts Lists
5.1 Inside the device

The mobile phase passes through a degassing membrane contained inside the degassing
chamber. The vacuum pump allows the dissolved gas in the mobile phase to permeate
through the degassing membrane and is exhausted out of the device through the 5
degassing chamber and vacuum tubing. Condensation of the mobile phase vapor in the
vacuum flow path is suppressed by taking outside air from the leakage mechanism into
the vacuum system.

Part Name Part No. Remarks

1 Degassing chamber,DGU-20AR 228-54502

DGU-403/DGU-405 11
 5 Instrument Components and Parts Lists

Part Name Part No. Remarks

2 Vacuum Pump, for DGU-20AR, LC 228-63035 　
3 Motor cable,DGU-20AR 228-54513
4 Control board,DGU-40 228-70250
5 Vacuum tube,DGU-40 228-70252 　
6 LED board,DGU-40 228-70251

5.2 Parts compatible table DGU-40 and DGU-20AR

DGU-403/405 DGU-20A3R/5R
Part No. Part Name Part No. Part Name
Degassing Degassing
228-54502 228-54502
chamber,DGU-20AR chamber,DGU-20AR
Vacuum Pump, for Vacuum Pump, for
228-63035 228-63035
DGU-20AR, LC DGU-20AR, LC
228-54513 Motor cable,DGU-20AR 228-54513 Motor cable,DGU-20AR
228-70250 Control board,DGU-40 228-54505 Control board,DGU-20AR
228-70251 LED board,DGU-40 228-54512 LED board,DGU-20AR
Vacuum tube,DGU-40 Vacuum tube,DGU-20AR
228-70252 (common use to 403 and 228-54506 (common use to 403 and
405) 405)
228-56687-42 SF Nut,1/8 228-46472 FLANGELESS FITTING
Flangrless fitting
－ None 228-46472-02
(2pieces),DGU-20AR
228-56687-43 SF Ferrule,1/8 228-54508-01 Ferrule,DGU-20AR
Ferrule
－ None 228-54508-02
(2pieces),DGU-20AR
228-44479-03 Stop joint,DGU-20AR 228-44479-03 Stop joint,DGU-20AR
Power cable Dsub 9 Power cable Dsub 9
228-53484 228-53484
pin,DGU-20AR pin,DGU-20AR
228-53486 Ground cable,DGU-20AR 228-53486 Ground cable,DGU-20AR
Alarm output Alarm output
228-53487 228-53487
cable,DGU-20AR cable,DGU-20AR
－ Front panel,DGU-40 228-42331-01 Front panel,DGU-20AR
Intake air filter Intake air filter
228-54509-02 228-54509-02
(2pieces),DGU-20AR (2pieces),DGU-20AR
Vacuum tube Vacuum tube
228-54656 228-54656
filter,DGU-20AR filter,DGU-20AR
228-42330-03 Main frame,DGU-20A3R
－ Main frame,DGU-40
228-42330-04 Main frame,DGU-20A5R
Vibration isolator Vibration isolator
228-54655 228-54655
rubber,DGU-20AR rubber,DGU-20AR

12 DGU-403/DGU-405
5.2 Parts compatible table DGU-40 and DGU-20AR

DGU-403/DGU-405 13
 6 Dismantling and assembling each unit

Before inspection and maintenance, unless instructed to do otherwise, be sure

to turn off the power to this instrument and disconnect the power cable.
Failure to do so may cause a fire, electric shock, or breakdown.

For the name and function of each component of the machine, refer to the
instruction manual.
This chapter focuses on what is necessary for maintenance. The procedure of
dismantling is explained in this chapter as well. Assembling should be performed
in the opposite order of dismantling. Cautions for assembling are given when
necessary.

Be careful not to get injured by the edges of the internal plates during
operation.

6.1 Periodic Checks and Maintenance

It is necessary to perform periodic inspections of this instrument to ensure its safe use.

When replacing parts, use the parts described in the DGU-403/DGU-405

instruction manual.
If any other parts are used, injury or malfunction may occur.

6.1.1 Preparation for Periodic Checks and Maintenance

• Replace the mobile phase solvent in the flow line with water.
• Clean off the front panel and main unit cover.

6.1.2 List of Periodic Checks and Maintenance

The replacement and check intervals listed in this table are merely guidelines. They are
not guaranteed time periods.

14 DGU-403/DGU-405
 6.1 Periodic Checks and Maintenance

There will be some variation depending on the usage conditions.

1 2 3
Check / Maintenance Item Remarks
year years years
Inspection/replacement of
○ 　 　 Replace the filter if it is badly soiled.
vacuum pump's intake filter
Inspection/replacement of
○ 　 　 Replace the filter if it is badly soiled.
vacuum tube filter
After confirming and removing the
Inspection/replacement of dew condensation in the vacuum line,
　 　 ○
vacuum pump*1 replace it if the vacuum pressure is
unstable.
Inspection/replacement of Replace the vacuum tube if it is
　 　 ○
vacuum tube*1 hardened or cracked.
After confirming and removing the
dew condensation in the vacuum line,
Inspection/replacement of
　 　 ○ check the inside of the vacuum tube.
degassing chamber*1
Replace it if droplets have
accumulated.
*1 Check every year after three years of use.

6.1.3 Precautions when not using for a long time

If the solvent delivery module is not to be used for a long time, turn "OFF" the power
to the unit and perform the following tasks.
• Remove the inlet tube and completely withdraw the liquid from inside the instrument
with a syringe or the like.
• After withdrawing the liquid, operate the degassing unit for at least 30 minutes to dry
the inside of the instrument. At this time, remove the stop joint of the liquid
inlet/outlet ports. 6
• Finally, plug the liquid inlet/outlet ports with the stop joints provided before storing
the instrument.

DGU-403/DGU-405 15
 6 Dismantling and assembling each unit

Be sure to withdraw the liquid inside the instrument by drawing it. Do NOT
pressurize the instrument.
• Particularly after using a buffer solution, the solvent can cause the
precipitation of salt and the growth of algae and microorganisms inside the
instrument (at the membrane). After use, thoroughly wash the interior with
distilled water or ion exchanged water and then pour in methanol or the
like to replace it. (Only suction operation using a device such as solvent
delivery module is allowed.)
• After replacement, withdraw the liquid (methanol etc.) inside the
instrument with a syringe etc. sufficiently.
• After withdrawing the liquid, operate the degassing unit for at least 30
minutes to dry the inside of the instrument. At this time, remove the
stop joint of the liquid inlet/outlet ports.
• When storing the instrument, plug the liquid inlet/outlet ports.
• When withdrawing liquid with a syringe or the like, slowly withdraw it at a
flow rate of 10 mL/min or less. A high flow rate may cause the degassing
membrane to be overloaded, resulting in shorter lifetime of the degassing
chamber.

If the cover or the front panel is soiled, wipe it off with a dry soft cloth or
tissue paper.
If they are badly soiled, wipe them with a cloth dampened with a neutral
detergent to remove the soil. Soak a piece of cloth with water and wring it
out to remove excess water. Use this cloth to wipe away all the remaining
detergent. Use a dry cloth to dry the exterior surface of the instrument.

6.2 Parts Replacement

6.2.1 Removing the Cover

1 Remove the screws (2 places) on the back of the instrument. Then, remove the
cover by sliding backward.

16 DGU-403/DGU-405
 6.2 Parts Replacement

6.2.2 Replacing the Vacuum Pump (228-63035)

1 Remove the screws (2 places) on the back of the instrument. Then, remove the
cover by sliding backward.

2 Remove the vacuum tube connected to the vacuum pump.

3 Remove the vacuum pump (with rubber feet) by loosening the screws (3 places)
securing the rubber feet of the vacuum pump.

DGU-403/DGU-405 17
 6 Dismantling and assembling each unit

4 Attach the rubber feetto the vacuum pump.

Rubber feet are ncluded in the vacuum pump (228-63035).

5 Install a new vacuum pump in the reverse order.

6.2.3 Replacing the Degassing Chamber (228-54502)

1 Remove the screws (2 places) on the back of the instrument. Then, remove the
cover by sliding backward.

2 Remove the vacuum tube connected to the degassing chambers.

18 DGU-403/DGU-405
 6.2 Parts Replacement

3 Remove the degassing chambers (with the metal plate) by loosening the screws
(2 places) securing the metal plate which holds the degassing chambers.

4 Remove the degassing chamber by loosening the screw (1 PC for each chamber).

5 Install new degassing chambers in the reverse order.

6.2.4 Replacing the PCB (228-70250)

1 Remove the screws (2 places) on the back of the instrument. Then, remove the
cover by sliding backward.

2 Remove all the cables connected to the PCB.

DGU-403/DGU-405 19
 6 Dismantling and assembling each unit

3 Remove the PCB by loosening the screws (4 places) fixing the PCB.

4 Remove the vacuum tube connected to the pressure sensor on the PCB.

5 Install a new PCB in the reverse order.

20 DGU-403/DGU-405
 6.2 Parts Replacement

6.2.5 Replacing theLED PCB (228-70251)

1 Remove the screws (2 places) fixing the main frame.

2 前面内部の2本のネジを取り外します。

DGU-403/DGU-405 21
 6 Dismantling and assembling each unit

3 Remove the connector of the cable connected to the LED PCB.

4 Remove the drain tube from the main frame.

5 Remove the main frame from the chassis.

22 DGU-403/DGU-405
 6.2 Parts Replacement

6 Remove the LED PCB by loosening the screws (2 places).

　　

7 Install a new LED PCB.

6.2.6 Replacing the Filters (228-54509-02) on the Vacuum Pump

1 Remove the screws (2 places) on the back of the instrument. Then, remove the
cover by sliding backward.

2 Remove the filters on the vacuum pump (2 places).

3 Attach new filters.

6.2.7 Replacing the Vacuum Tube Filter (228-54656)

1 Refer to steps 1 to 4 in "6.2.4 Replacing the PCB (228-70250)" and remove the
PCB.

DGU-403/DGU-405 23
 6 Dismantling and assembling each unit

2 Remove the vacuum tube filter.

3 Attach a new vacuum tube filter.

24 DGU-403/DGU-405
7 Maintenance and Calibration
7.1 Screens of Solvent Delivery Module
The vacuum pressure and the operating time can be monitored from the display of the
solvent delivery module to which the degassing unit is connected.
Refer the instruction of solvent delivery module for detail.

7.1.1 Monitoring Screens

n Monitoring Vacuum Pressure in Degassing Unit 《DEGAS PRESSURE》

The screen displays the vacuum pressure of

the degassing unit connected to the
instrument.
• [(OK)] is displayed when the vacuum
pressure is at a normal value.
• [(NG)] is displayed when the vacuum pressure is at an abnormal value.
• [Disconnected] is displayed when a degassing unit is not connected.

To activate this function, supply power from the instrument to the degassing unit and make
sure that the pressure signal cable is connected correctly. If more than one pump is used,
be sure to connect the power cable and pressure signal cable to the same pump. For how
to connect, refer to the System Guide.

7.1.2 VP Function Screens / Maintenance Information Group

n Displaying the operation time of the degassing unit 《DGU OP TIME》

The screen displays the operation time of

the degassing unit connected to the
instrument. 7
When the operation time is input, count
of the operation time of the degassing
unit starts from the input time. The input set value is stored in the maintenance log.
If [999999 h] is set, the display shows "******" and the operation time will not be
counted.

DGU-403/DGU-405 25
 7 Maintenance and Calibration

To activate this function, supply power from the instrument to the degassing unit and make
sure that the pressure signal cable is connected correctly. If more than one pump is used,
be sure to connect the power cable and pressure signal cable to the same pump. For how
to connect, refer "4.1.2 Connecting to the Pump".

• The shutdown function of SCL-40/CBM-40/CBM-40lite, or LC-Workstation can

stop the degassing unit. For details, refer to the instruction manual of the
system controller or LC-Workstation.
• Approximate time to replace the vacuum pump of the degassing unit is 3 years
or 8000 hours of operation time.

26 DGU-403/DGU-405
8 Troubleshooting and Corrective Action
8.1 Troubleshooting

8.1.1 Test Points on Control Board

n Checking the input voltage

Measure TP1 and TP2 (GND) with a tester and confirm that they are 24 V ± 10%. Replace
the power cable if there is no power.

n Checking the output voltage of vacuum pressure

Measure TP3 and TP2 with a tester and check the voltage output.

Condition Voltage (mV) Vacuum Pressure (kPa)

Normal 100 6.7
Error threshold 180 10.7
(Reference) Atmospheric
2000 101.3
pressure

n Checking the rotation speed of vacuum pump

3.3×1000/3200×60=61.2rpm
Set the tester to the frequency range and measure TP5 and TP2 (GND). The number of
revolutions is calculated as follows:.
8
Number of revolutions (seconds) = Frequency (Hz)/3200 (micro steps) × 60 (rpm)
(Calculation Example) When the measured value is 3.3 kHz
3.3 x 1000/3200 x 60 = 61.2 rpm

Condition Rotation Speed

Factory (dry condition in vacuum system) Approx. 100 rpm
Maximum 350 rpm

DGU-403/DGU-405 27
 8 Troubleshooting and Corrective Action

When the rotation speed is 350 rpm

Cause: The performance of the pump is degraded.
(1) Leakage of liquid or condensation from the chamber
Action: If liquid is leaking, replace the chamber.
If condensation occurs, remove the vacuum tube from the chamber, turn on the power,
and operate the pump. Dry the inside of the vacuum system and pump.
(2) When there is no liquid leakage or condensation from the chamber.
Action: Replace the vacuum pump because parts may be degraded.

When the rotation speed is under 50 rpm

Cause: The filter is clogged.
Action: Replace the vacuum pump filter. If this does not work, replace the vacuum tube
filter.

The number of revolutions of the vacuum pump varies slightly depending on the
type of solvent remaining in the degassing membrane (Because the permeability
of the degassing membrane is different), but normally it operates at 60 to 150
rpm.

8.1.2 Precautions for Solvents

n Water/Acetonitrile/Methanol

If these solvents are left in the degassing chamber with the power supply turned off,
dew condensation may occur in the vacuum system and a vacuum pressure error may
occur upon restart.
In this case, the vacuum pressure can be recovered by turning off the power supply of
the equipment and operating the vacuum pump until dew condensation of the vacuum
system is removed.

n Normal phase solvents such as Hexane/THF

These solvents have a higher vapor pressure than the solvents shown above, and have a
higher affinity for the degassing membrane. As a result, solvent vapor tends to enter the
vacuum system and condensation tends to occur.
In this case, remove the piping connected to the degassing chamber and remove the
liquid in the degassing chamber before operating for 2 -3 hours.

n Chlorine-Containing solvents such as Chloroform/Dichloromethane

Because the SUS stent is used to fix the degassing membrane, if these solvents are left
in the degassing chamber, water in the air reacts with the solvent to generate
hydrochloric acid, which causes rust in the SUS. Therefore, after using these solvents,
replace the flow path with a compatible solvent such as IPA or ethanol, and then turn
off the power.

28 DGU-403/DGU-405
9 Wiring Diagram
DGU-20AR service manual
9

DGU-403/DGU-405 29
 10 Maintenance Parts
Part No. Part Name Remarks

228-54502 Degassing chamber,DGU-20AR

228-63035 Vacuum Pump, for DGU-20AR, LC

　
228-54513 Motor cable,DGU-20AR

228-70250 Control board,DGU-40

228-70251 LED board,DGU-40

Vacuum tube,DGU-40
228-70252
(common use to DGU-403/DGU-405)

228-56687-43 SF Ferrule,1/8

30 DGU-403/DGU-405
 Part No. Part Name Remarks

228-56687-42 SF Nut,1/8

10

228-54509-02 Intake air filter(2pieces),DGU-20AR

228-54656 Vacuum tube filter,DGU-20AR

228-54655 Vibration isolator rubber,DGU-20AR

DGU-403/DGU-405 31
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