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Aoac 945.16

This document describes AOAC Official Method 945.16 for determining oil content in cereal adjuncts using a petroleum ether extraction method. Key details include: - The method extracts a 5-10g ground sample in a Soxhlet or other suitable extractor using petroleum ether for up to 6 hours. - The extract is filtered and the solvent is distilled or evaporated at under 100°C. - The residue is dried in an oven at 100-105°C for 1 hour. - The oil content is reported as a percentage to the second decimal place.

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610 views1 page

Aoac 945.16

This document describes AOAC Official Method 945.16 for determining oil content in cereal adjuncts using a petroleum ether extraction method. Key details include: - The method extracts a 5-10g ground sample in a Soxhlet or other suitable extractor using petroleum ether for up to 6 hours. - The extract is filtered and the solvent is distilled or evaporated at under 100°C. - The residue is dried in an oven at 100-105°C for 1 hour. - The oil content is reported as a percentage to the second decimal place.

Uploaded by

Diana
Copyright
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  • AOAC Official Method 945.16: Oil in Cereal Adjuncts

27.4.

04
AOAC Official Method 945.16
Oil in Cereal Adjuncts
Petroleum Ether Extraction Method
First Action 1945
Final Action
A. Reagent
Petroleum ether.—AOCS. Initial boiling temperature, 35°–38°C;
dry-flask end point, 52°–60°C; ³95% distilling at <54°C, and £60%
dis till ing at <40°C; specific grav ity at 60°F, 0.630–0.660;
appearance, colorless; evaporation residue, £0.0011 g/100 mL;
doctor test, sweet; Cu strip corrosion test, noncorrosive; only trace
of unsaturated compounds permitted; residue in distilling flask,
neutral to methyl orange; blotter strip odor test, odorless within
12 min; aromatic compounds, no nitrobenzene odor; saponification
value, <1.0 mg KOH/100 mL.
Make distillation test according to ASTM method D216-54 and
make blank determination by evaporating 250 mL with ca 0.25 g
stearin or other hard fat (previously brought to constant weight by
heating) and drying as in actual determination. Blank must be
£3 mg.
B. Determination
(Cau tion: See Appendix B, safety notes on dis til la tion,
flammable solvents, and petroleum ether.)
Accurately weigh 5–10 g test portion ground as in 935.30D (see
27.3.07). Without previous drying, extract in Soxhlet or other
suitable extractor with petroleum ether for £6 h. Filter extract
through small, hardened paper into weighed vessel, washing paper
finally with small portion of hot fresh solvent. Distil or evaporate
solvent at temperature £100°C and dry vessel containing residue in
air oven 1 h at 100°–105°C. Report as % oil to second decimal place.

ã 2005 AOAC INTERNATIONAL

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