Operating & Maintenance Manual: Safefast Light
Operating & Maintenance Manual: Safefast Light
Via Merendi, 22
20010 Cornaredo (MI)
Tel. +39.02.93.991.92
Fax. +39.02.93.991.608
E-mail: [email protected]
OPERATING &
MAINTENANCE MANUAL
SafeFAST Light
INDEX
1 GENERAL ..............................................................................................................................................3
2 INSTALLATION .....................................................................................................................................4
2.A INSTRUCTIONS AND CONTROLS ON DELIVERY .............................................................................4
2.B INSTALLATION REQUIREMENTS .......................................................................................................4
2.C ELECTRIC CONNECTIONS AND PLACEMENT OF THE WORK SURFACE .......................................6
2.D POSITIONING OF THE CABINET ON THE SUPPORTING TABLE .....................................................6
3 OPERATION PRINCIPLES....................................................................................................................8
4 OPERATION ..........................................................................................................................................9
4.A SCOPE ..................................................................................................................................................9
4.B CONTROLS OF SYSTEM AND PERFORMANCES .............................................................................9
4.C REMOTE SIGNALS (OPTIONAL) ............................................................................................................9
4.D SYMBOLS OF CONTROL BOARD .....................................................................................................10
4.E OPERATOR MENU .............................................................................................................................14
5 WASTE TREATMENT OF DISPOSAL ................................................................................................19
6 LIMITATIONS ......................................................................................................................................20
7 OPERATING PROCEDURES..............................................................................................................21
7.A PRELIMINARY CHECKS.....................................................................................................................21
7.B SWITCHING ON THE SAFEFAST LIGHT CABINET...............................................................................21
7.C SWITCHING OFF THE SAFEFAST LIGHT CABINET.............................................................................21
8 MAINTENANCE ...................................................................................................................................22
8.A INSTRUCTION FOR DAILY CLEANING OF VERTICAL LAMINAR FLOW CABINETS (CARE OF
USERS) 22
8.B HEPA FILTER REPLACEMENT (CARE OF TECHNICAL ASSISTANCE PERSONNEL) ..................................23
8.C REPLACEMENT OF MOTOR-FAN (BY TECHNICAL ASSISTANCE PERSONNEL) ......................................23
8.D REPLACEMENT OF FLUORESCENT LAMPS (BY USER) ...................................................................24
8.E REPLACEMENT OF UV GERMICIDE LAMP (OPTIONAL) (BY USER) ....................................................24
8.F LIST OF SPARE PARTS .....................................................................................................................25
9 TROUBLESHOOTING - PROBABLE MALFUNCTION CAUSES ......................................................26
10 TRANSPORT, PACKING AND STORAGE INSTRUCTIONS .........................................................27
11 ADDITIONAL INFORMATION .........................................................................................................28
11.A GUARANTEE ......................................................................................................................................28
11.B ADDRESS OF TECHNICAL ASSISTANCE (BY OF DISTRIBUTOR) ........................................................28
12 TESTS PERFORMED ACCORDING TO EN 12469 STANDARD...................................................29
12.A MEASUREMENT OF LAMINAR AIRFLOW VELOCITY......................................................................29
12.B MEASUREMENT OF THE EXHAUST AIR VELOCITY .......................................................................30
12.C MEASUREMENT OF THE NOISE LEVEL...........................................................................................30
12.D MEASUREMENT OF VIBRATION.......................................................................................................30
12.E CONTROL OF ALARMS......................................................................................................................31
12.F DEHS TEST FOR MAIN AND EXHAUST HEPA FILTER ....................................................................32
12.G INSTRUCTIONS FOR THE CLEANING TEST OF BIOLOGICAL SAFETY CABINETS .....................33
12.H EFFICIENCY OF CONTAINMENT OF THE CABINET – KI METHOD ................................................34
13 GUIDE-DRAWING FOR MAINTENANCE OPERATIONS...............................................................37
14 SAFEFAST LIGHT FRONT DIAGRAM............................................................................................40
14.A SAFEFAST LIGHT SIDE DIAGRAM....................................................................................................41
15 ASSEMBLY DIAGRAM OF SUPPORT TABLE ..............................................................................42
16 SENSORS LIST ...............................................................................................................................44
17 WIRING DIAGRAM ..........................................................................................................................45
18 DECLARATION OF CONFORMITY ................................................................................................49
1 GENERAL
Vertical laminar airflow benches with partial exhaust and protection barrier, the SafeFAST Light cabinets,
are designed to protect both the material to be manipulated from contamination and to protect the
operator and the environment from microbial contamination hazards.
The dusted, filtered and sterile air passing through the main HEPA filter ensures optimum airflow
laminarity on the work surface, thanks to its even perforation and the frontal air barrier. The system is
designed to create a protective barrier and prevent exchanges between the internal, potentially biologically
contaminated air, and the outside.
SafeFAST Light cabinets are Class II Biohazard cabinets, according to the definitions now adopted by all
the main international standards (example: EN 12469:2000) Suitable for the treatment of class CDC-2/3,
DHSS-B2/B1 group 1,2,3 microorganisms as per D.L. 81/08 “Safety in the working environment” and
wherever a product-personnel-environment cross-protection is required.
Especially suitable for applications such as:
• Manipulation of eziologic agents having a known pathogenicity on humans and animals.
• Presence of high concentrations of biologic materials.
• Presence of agents likely to cause genetic alterations or synergetic activities with other materials.
• Oncogenic viruses.
Do not manipulate the products on the slotted part at the entry of the chamber, but in the middle of the
work surface.
The performances of the cabinets are detailed in the TESTING CERTIFICATE below, in compliance with
the requirements of:
– EN 12469 (2000)
Furthermore SafeFAST Light cabinets fulfil the armonized standards EN 61010-1 as well as EN 61326
according to the applicable European directives regarding the CE marking.
SafeFAST Light cabinets comply with the above-mentioned standards ONLY if the instruments connected
to the electrical socket positioned inside the work chamber are "CE" marked or in any case it meets the
above mentioned standards aiming to avoid any electromagnetic interference.
All Faster’s cabinets are provided with high insertion loss filters.
2 INSTALLATION
LEGENDA: THIMBLE
2
1. Exhaust air from the cabinet.
2. Air from the environment.
3. Bleed Air (100÷200 m /h more than the exhaust
3
1
air flow rate from the cabinet) to a dedicated
exhaust fan (in the electronic board a voltage
free contact is available –see electrical diagram- Microbiological Safety Cabinet
to check when ventilation is ON).
The quantity of heat generated by the cabinet, if the air is not extracted outside the room, is 70W.
This heat value must be added to the heat generated by any instrument (eg. connected to internal socket)
used by the client in the work chamber.
ATTENTION: the installation must be done by technicians authorized by Faster S.r.l. or by the
official distributor.
The electrical connection of the SafeFAST Light Biohazard cabinet is made by connecting the power cable
located on the upper of the right side of the cabinet to a suitable power point (see technical table) . When
the cabinet is connected, the green light on the control panel switches on (see chapter 4E).
If stipulated by local legislation, insert upstream of the power line an automatic protection overload switch
provided with a differential relay, with a rated switching voltage no greater than 30 mA.
3 OPERATION PRINCIPLES
The pressurized air pushed by the motor-fan goes through the absolute filter and then downwards in
laminar flow into the working chamber (A). From here, through the perforated surface, mixing with the
external air entering the cabinet from the front aperture (B), then the air is sucked into the intake up
channel situated at the rear of the work chamber and part of the air (about 10÷15% of the total) is
exhausted through the exhaust HEPA filter located in the upper part of the cabinet (C), this exhausted air
generates the pickup of air from the outside (front barrier) ensuring adequate air recycling and keeping a
reduced thermal drift.
A
B
4 OPERATION
4.A SCOPE
The vertical laminar airflow biosafety cabinet SafeFAST Light is manufactured in compliance with
international standards for the protection of the material, the operator and the environment against
biological hazards of Class II and is suitable for the manipulation of low- and middle- risk pathogenic.
The front panel of hardened glass is hinged to facilitate the introduction of bulky objects,
The electronic control board can be improved with an output signal: it is possible to obtain a 12 Vdc output
to connect a led light or alternatively a Normally Open voltage free contact to be connected to an external
circuit. This signal can be set in three different modes:
Motor ON:
The signal starts when ventilation is turned ON and stops when ventilation is OFF.
Alarm:
That signal is ON in case of any flow alarm and it is OFF when air flow rate is in the correct range.
LAF OK:
That signal is ON when air flow rate is in the correct range and it is OFF in case of any flow alarm.
List and description of all the symbols and controls of the control panel:
4 15 11
1 2 3 5 6 7 8 9 10 12 13 14
1 MAIN SWITCH:
Position "0" in the "0" position, the green light of the mains voltage is on (4); the LCD
displays the model name. In this position the operator can activate only
the fluorescent light (12), the UV lamp (3) and the power outlet (13) (with
plug installed) and can activate the data stored in the microprocessor by
pressing the “Right Arrows” key (9-10).
Position "I" Press key "I" [1], insert the password using the small numbers in the right
lower corner of the keys and press SET. Default password is 5 [9] – 4 [6].
When the password is typed in the green led of the switch lights up and
the cabinet starts operating, the motor-blower is powered and first
"CHECK PANEL" then "STAND-BY" appears on the display till the air flow
reaches the pre-set value. In addition, an audible alarm will sound
intermittently during this stand-by period, alerting the operator not to start
working yet. When the audible alarm stops and the message "STAND-
BY" disappears from the display, the cabinet is ready for use. The air
velocity is displayed.
NOTE: In any case, it is advisable to wait 5 minutes before starting work.
2 STAND BY (speed reduction) By pushing the corresponding red key the password (the same to
start the ventilation) is requested. Once confirmed the password the
function is enabled. When it is enabled, the corresponding red LED lights
up and, for single fan cabinets, the air speed is about 30% lower than
their nominal speeds. The light cannot be switched on. If they are on, they
switch off automatically and the following two messages appear
alternatively:
>>>ATTENTION<<<
DANGER
and:
REDUCED AIRFLOW
>>>DO NOT WORK<<<
The “SPEED REDUCTION” function can be enabled only with the main
switch in position “I”. Only the power supply (13) can be operated.
3 UV (optional) Yellow key to switch on UV. This supplies the UV lamp in “manual” mode;
when enabled, the display (if installed) shows "UV on". The UV lamp
switches on only if the cabinet is off, the lighting of the cabinet is off and
the front window is completely closed.
By pushing the relevant key you are requested to set the U.V working
time. The timer resolution is 1 minute and the maximum time is 180
minutes (3 hours). The display will show the latest pre-set value, which
can be changed using the “arrow” keys; when the time has been set,
press the “SET” key to confirm. At this point the yellow LED lights up, the
UV lamp is activated and the countdown starts. During the cycle the
following message appears:
UV TIMER
UV Timer (min) xxxx
4 LINE The green mains light switches on if the unit is connected to the mains
and the line is live
5-6UP/DOWN ARROWS Use the arrow keys to scroll the menu, to program changing parameters
and to put in the password. Two passwords are programmed:
1) to start the cabinet and to enter the operator menu
2) to enter the main menu to change the data input (allowed only to
authorized technical staff – service – because unsuitable interventions
can cause troubles and incorrect operation of the cabinet. If installed with
the up/down arrows it is possible move the sash window
7 ESC ESC key deletes the operation of data input and goes back to the starting
condition.
When an alarm condition occurs, which is shown also by the message
appearing on the LCD. By pushing "ESC" (if enabled) the alarm stops
sounding. If the cause of the alarm is not resolved after 2 minutes the
buzzer starts to sound again.
8 SET SET key lets you enter the different functions or confirm the data input
going back to the upper level.
9-10LEFT/RIGHT ARROWS Use the arrow keys to scroll the menu: if pressed the following data will
appear on the display:
UV Lamp Residual Lifetime: Shows the operating time of the UV lamp
pre-set by the user with the appropriate keys. The LCD will display (for
example) "UV TIME=XXXX h" . When such time is over, the message
"UV LIFETIME OVER" will appear on the line below.
1, 2, 3 Residual lifetime of filters : it is the operation time of the filters
installed in the cabinet that can be programmed by the user.
The LCD will display (for example)" RES. TIME FILTER 1=XXXX:XX
h:min". When such time is over, the message "CHECK FILTER (i.e.) 1".
will appear on the line below.
The filters installed in the cabinet follow the numbering listed below
MAIN HEPA 1
Operating Time: Shows the operating time of the cabinet from the
moment when the main switch is positioned on "I"
The LCD will display (for example) "WORK TIME=XXXXXh”. This value
cannot be reset.
11 ALARM When an alarm occurs the red LED lights up.
12 LIGHT This switches on the fluorescent light; when enabled, the display shows
"Light on". Switching on the fluorescent light automatically the UV lamp
switches off.
13 SOCKET This supplies voltage; when enabled, if the PCB for the automatic
regulation is installed the display shows "POWER ON". The global current
for all the sockets installed on the cabinet is 4 Amps.
14 FUEL GAS Due to the small dimension of SafeFAST Light cabinet the use of fuel gas
with Bunsen burner is forbidden.
15 DISPLAY Rearlight liquid crystal "LCD" display composed of 2 lines of 20 characters
each showing the operating parameters and alarms.
It’s possible enter the operator menu, with the cabinet in stand-by mode and when the cabinet is switched on, by pressing at the same time the keys “ESC” (7) and “ARROW UP” (6).
Entering the operator menu while the ventilation is running it’s possible to set only the “ALARM CLOCK” and the “TIMER”.
STAND-BY MODE
PASSWORD
ESC + UP arrow
OPERATOR MENU
Alarms display
• where XXXX shows the number of the hours set for lifetime of the UV lamp.
• Use the “up and down arrow” keys to adjust the hours parameter
• Then press the "SET" key [8] to confirm the data and/or go back to previous menu
• To conclude programming, press ESC” [7] key.
LANGUAGE SELECTION
LANGUAGE
English
• With the “up and down arrow” keys select the desired language (Italian, English, French, German,
Spanish).
• Press the “SET” key to confirm and exit the “LANGUAGE” menu.
• press “ESC” [7] keyto go out.
• Use the “left and right arrow” keys to select the desired parameter and set it using the “up and down
arrow” keys
• Then press the "SET" key to confirm the data and/or go back to previous menu
• To conclude programming, press ESC” [7] key.
PASSWORD CHANGE
ALARM CLOCK
• Use the “left and right arrow” keys to select the desired parameter and set it using the “up and down
arrow” keys
• Then press the "SET" key to confirm the data and/or go back to previous menu
• Press “ESC” to abort the procedure
• When the set time is reached the buzzer start to ring and the display show this message
ALARM CLOCK
ALARM
• Press the “ESC” key to silence the acoustic signal
TIMER SET UP
Timer set up
Set up XXXX
• Use the “left and right arrow” keys to select the desired parameter and set it using the “up and down
arrow” keys
• Then press the "SET" key to confirm the data and/or go back to previous menu
• Press “ESC” to abort the procedure
• At the end of the countdown the buzzer start to ring and the display will show this message:
TIMER
ALARM
• Press the “ESC” key to silence the acoustic signal
UV TIMER
UV TIMER
Duration XXXX
• Use the “left and right arrow” keys to select the desired parameter and set it using the “up and down
arrow” keys
• Then press the "SET" key to confirm the data
• The display shows:
UV TIMER
DATE XXXX
• Use the “left and right arrow” keys to select the desired parameter and set it using the “up and down
arrow” keys
• Then press the "SET" key to confirm the data and/or go back to previous menu
Appropriate disposal of this product will help to prevent potential negative effects on health and environment
and to promote re-use and / or recycling of materials of the equipment.
The improper disposal of the product by holder involves the application of sanctions in accordance with the
regulations in their own country.
INFORMATION FOR USERS OUTSIDE THE EUROPEAN UNION
This symbol is valid only in the European Union If you want to dispose this product, contact your local
authorities or dealer and ask for the correct method of disposal.
6 LIMITATIONS
PRECAUTIONS for the correct use of the cabinet
Listed below are the most important guidelines to be followed and the main substances to be avoided to
ensure the correct use of the SafeFAST Light cabinet:
NEVER USE chlorine-based substances (e.g. sodium hypochlorite) as they are corrosive for the metal
structure of the cabinet, and in particular for stainless steel parts.
When the nature of the work carried out under the cabinet changes completely or following an accidental
spilling of pathogenic material, STOP working and start again only after having cleaned and sterilized the
cabinet,
DO NOT use ethanol as a sterilizing substance if a heat source is used under the cabinet,
DO NOT use cosmetic powders, nail polish, hairspray or cosmetics in general during work,
DO NOT eat, drink or smoke in the work zone,
AVOID substances that release explosive vapor.
Before opening the front window be sure that the inside of the cabinet has been properly decontaminated; in
fact this part can be potentially dangerous for the operator’s hands as well as for the laboratory air, which
comes directly into contact with, after opening the front window.
ATTENTION: the UV radiations emitted by the germicidal UV lamp can cause erythemas and
conjunctivitis. Avoid exposure of skin and eyes to direct radiation
7 OPERATING PROCEDURES
– that the cabinet has been sterilized in case of change in the nature of work to be carried out
8 MAINTENANCE
8.A INSTRUCTION FOR DAILY CLEANING OF VERTICAL LAMINAR FLOW CABINETS (care
of users)
Clean the outside of the SafeFAST Light cabinet, made of varnished iron-carbon sheet steel, using a damp
cloth soaked in soapy water or some other commonly available products for varnished metal surfaces. The
procedure to follow is:
NEVER USE CHLORINE-BASED SUBSTANCES
switch off the cabinet and disconnect the feeding cable;
clean carefully all the varnished surfaces using a damp and squeezed cloth with soapy water or equivalent
product.
wipe all the surfaces using a soft cloth absolutely not abrasive.
Clean/sterilize the internal work chamber with a bactericidal agent chosen according to the type of pathogenic
micro-organism manipulated (e.g. hydrogen peroxide, ethyl alcohol, ethanol, phenolic compositions,
aldehydes, quaternary ammonium salts, etc.).
The procedure to follow is:
switch off the cabinet and disconnect the feeding cable;
protect your hands wearing gloves if required by the bactericidal agent you are using
spray the bactericidal agent on the side walls, the back wall and the work surface in a such uniform way to
avoid leaving not sprinkled zones.
do not spry the filtering upper zone to avoid damaging this part
wipe carefully all the sprinkled zones using clean blotting paper. Be sure to remove any residual of the
bactericidal agent.
If pathogenic material has been spilled during the work session, sterilize the work surface, then remove it
using the small handles mounted on the sides. Sterilize the spilled materials contained in the collection basin
located under the table and remove all spilled material by means of absorbent paper.
For thorough cleaning we suggest the use of a 70% ethanol solution or a solution of other bacteriostatic
agents.
WARNING: Never use solutions containing free chlorine (for instance, sodium hypochlorite),
which cause corrosion to steel and stainless steel, resulting in irreparable damage to
the cabinet structure.
For the safety of the personnel and the environment, the use of PVC gloves is recommended as well
as the collection of the replaced HEPA filters in polyethylene bags.
KNOB
METAL PLATE
7) Remove the metal plates from the clogged LAF HEPA filter and position them on the new HEPA
filter
8) For reassembly reverse the above instructions, taking care of:
a. not tightening knobs [9] too much
b. replace the old filters gasket
N.B : Gaskets shall be EPDM of the closed-cell type and have no interstices in the joining points.
Gaskets from neoprene, hypalon, etc. may be replaced by CAF-type non-adhesive silicone.
Important: After replacing filters, proceed to the calibration of motor-fan velocity, and perform also a
control with a particle-meter
N.B. During the installation of the new lamp, special care should be paid to handling, avoiding to
leave fingerprints on the lamp.
Cabinet does not work – the electricity supply has – check the voltage input to the
been cut off at the mains cabinet
Alarm: “HEPA filters check” Main HEPA filter clogged Replace HEPA filter
Alarm: “No encoder input LAF” No signal from LAF flow Replace the LAF sensor
sensor
ATTENTION: Disconnect the power and sterilize the unit before performing any of the following
operations
The following instructions are essential if the end user needs to transport, pack or store a cabinet after a
period of routine use (e.g. laboratory/plant relocation):
- If the cabinet exhausts to the outside of the building or is connected to the intake channel of the air
treatment system, remove the connection tube from the cabinet to the outside or cabinet to the air
treatment system. Be careful not to damage or cover with dust (or any other material) the exhaust filter
of the cabinet
- Remove the work surface and cover it with a protective film
- Close the cabinet front opening
If the cabinet is to be moved from one laboratory to another within the same building:
- on a table with wheels: it is sufficient to put the cabinet on the table; do not place it on one side or on the
back panel.
- by a forklift: put the cabinet on a pallet to ensure good stability and to protect the basin under the cabinet
and the front window against damage in transit
Take care not to damage protruding parts (e.g.: gas/vacuum taps, exhaust duct) when passing through
doors/windows
If the cabinet is be kept temporarily unused at final destination ,cover the cabinet itself with a protective film
(pluriball or expansible film) taking care to protect also the exhaust filter, especially from dust
We recommend extreme caution in long-distance moving carried out by forwarding agents (e.g. change of
address): we suggest that you use the original packaging supplied by the manufacturer with the cabinet.
Packaging characteristics:
During transport pay attention to maintain the package in vertical position (i.e. the pallet at the bottom)
The cabinet (with or without the package) must be kept in a place ensuring following environmental
conditions:
¨ -min. temperature: 0°C
-max. temperature: 70°C
-max. humidity: 90%.
11 ADDITIONAL INFORMATION
11.A GUARANTEE
The guarantee for vertical laminar air-flow benches model SafeFAST Light is 24 months from date invoice.
The limitations of the guarantee established by Faster S.r.l. concern, besides the cases indicated in Chapter
7 relating to improper use of cabinet by user, also several contra-indications in the instruction manual, among
which the following are reminded:
¨ installation in a place not conforming to the instructions
¨ wrong feed voltage
¨ bad earth connection
¨ utilization of chlorine or its derivatives, incompatible with stainless steels, for cleaning the cabinet,
¨ tampering or changes made by the client
¨ tampering cabinet with any type of tool
¨ wrong connection between electric socket and feed cable
The LAF velocity is measured at 10 cm. over the bottom of the front window. The standard provides for the
reading in at least 8 points:
In our tests the readings are taken in 12 points as above-diagrammed. Each one of the 12 readings lasts at
least 1 minute. The velocity is measured with a hot-wire thermoanemometer with the following features:
Precision (of the device including the probe) +/- 0.05 m/s +/- 5%
Resolution m/s
Measure the average velocity on the exhaust HEPA filter by means of an anemometer with the following
features :
Resolution m/se
VI = (V x S1)/S2
For a correct testing the background noise in the laboratory with the cabinet off should not exceed 55 dBA.
The microphone of the phonometer must be placed at 1 m from the centre of the working aperture of the
cabinet between the glass and the work surface or 1m from any part of the cabinet.
The sound pressure level must not be over 65 dBA.
The measure taken must be reported in then "Test report".
Take the measures with a vibration analyser, which is to be placed in the middle of the work surface.
The test must be performed firstly when the cabinet is off and then when the cabinet is working.
The difference of the taken values is the real datum of the cabinet vibration. This value should not exceed
0.005 mm RMS
Record the data in the “Test Report”
OTHER ALARMS
With the help of FASTER’s technicians also the alarms for LAF and protection barrier velocity below the
minimum threshold or over the maximum threshold can be checked
REAGENTS:
Suitable measures must be taken to prevent or reduce the operator’s exposure to aerosol.
TEST PROCEDURE:
The photometer must be used at a threshold sensitivity specific to the test aerosol of 0.0001 micro-g/l and
capable of measuring aerosol concentrations up to about 80 micro-g./l.
The test must be performed as follows:
– Place the aerosol generator in such a way that the aerosol produced is immediately catched by the
barrier airflow and then recycled into the intake channel
– Switch the generator on and adjust the photometer to give a reading of 100% when sampling this
concentration
– Ensure that the threshold of reading sensitivity of the photometer is 0.0001 microg./l.
– Operate the cabinet with airflow velocity as per specs.
– Using a sampling probe attached to the photometer scan the main and exhaust filter faces, seals and
construction joints, moving the sampling probe at not more than 30 mm/s
– Check the values read by the photometer constantly, in order to detect possible leakage.
– Acceptance criteria: downstream reading must be less or equal to 0.01%
ATTENTION: Before carrying out the cleaning test wear the suitable rubber gloves to avoid direct
contact with parts potentially contaminated.
The first step of the test is to pour some drops of milk inside the work chamber on several points included
those more difficult because near the edges and corners.
Then the whole work chamber must be cleaned and brushed carefully, at first by using a lukewarm solution
of water and soap (for the cleaning use a sponge and in the most critical points a brush) and then, after
wiping the whole chamber, isopropylic alcohol.
The check that all contaminants have been removed can be performed by means of a microbiological test
called IGIENTEST HACCP 100, which is able to find out the presence of residual proteins and sugar, or
equivalent
Method:
1. Add a drop of B Reagent (included in the test kit) in a test tube already containing A reagent
2. Shake the test tube gently: the solution will become green. Move the test tube into its support inside the
kit
3. (N.B.: do not close the test tube with the thumb because the contact with the skin could distort
the result. Use the cap)
4. Take a pad, being careful not to touch the end. The pad must be used only once: its re-use gives wrong
results. If the surface to be tested is dry, add one or two drops of the hydrating solution included in the
kit. Do not use water to wet the pad
5. Pressing firmly, rub the pad on the surface which is to be tested repeatedly. Do not touch the end of the
pad in fibre in order not to distort the results
6. Move the pad into the test tube previously prepared. The pad must be well soaked in the solution. Wait
10 minutes for the reaction.
7. After about 10 minutes the reaction generates the colour, which is to be interpreted according to the
following table:
The value of n is determined from an examination of the developped and dried filter membrane,
For a biological safety cabinet, which is to pass the containment test, the total number of spots of potassium
iodide on the membrane can be easily counted by examining each filter with x 10 magnifier at disposal
The N value (number of generated spots of potassium iodide) is calculated by the expression
N = 3.1 x 107 x M
where
M = quantity of potassium iodide expressed in ml dispersed by the aerosol generator (20 ml)
This expression incorporates the constant derived from droplet size, flowrate and velocity of disk rotation.
3
The sampling flowrate V is 100 dm /min when the vacuum on the air sampler is 8” of water column (200 mm
of H2O)
Required tests: at least 5 consecutive tests must be performed, each of one must give a protection factor not
5
less than 10 estimated for each sampling.
BACKGROUND TEST
Before performing the test on Biological Safety cabinets make sure that the environment is not contaminated
by Potassium iodide.
Proceed in performing the tests in the same way as in the measurement of the protection factor.
A count of more than 5 spots in one of the two membranes during a 10 min test should be regarded as
unsatisfactory
Be careful that the tweezers used for placing the membrane in the palladium chloride solution are not used
for loading the air samplers.
These factors take into account the percentage of the total surface of the membrane area on which the
particles can deposit.
LEGENDA
1 Work surface
2 Work chamber
3 Exhaust HEPA filter
4 Frontal panel
5 Frontal panel screws
6 Upper panel screws
7 Motor fan
8 Motor fan screws
9 Knobs
10 LAF HEPA filter
11 Lamp
12 Frontal glass
13 Exhaust anemometric fan
14 Exhaust plenum
15 LAF anemometric fan
x4 x4
2 3
16 SENSORS LIST
J3-PWLAF S3
J2- PWLAF S4 Proximity switch for U.V.
17 WIRING DIAGRAM
18 DECLARATION OF CONFORMITY
The undersigned legal representative of the company Faster S.r.l. hereby declares that the follow products:
SafeFAST Light
2004/108/EC Directive of the European Parliament and of the Council on the approximation of the
laws of the Member States relating to electromagnetic compatibility
2006/95/EC Directive of the European Parliament and of the Council on the harmonisation of the
laws of Member States relating to electrical equipment designed for use within certain
voltage limits
and, according to the above-mentioned directives, the CE IIA mark has been applied.
The undersigned also declares that the person who is authorised to compile the relevant technical
documentation is Mr.:
Faster S.r.l.
Maria Giulia Turzi
Chairman of the board