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CENTRO ESCOLAR UNIVERSITY

Manila * Makati * Malolos

SYLLABUS ON PCCH104 - BIOCHEMISTRY

Course Number : PCCH104 Course Descriptive Title : Biochemistry

No of Units : Lec: 3 Lab: 2 No of Hours per Week : Lec: 3 Lab: 6
Pre-requisites : PCCH102 Organic Chemistry
Course Description:
The course is designed to acquaint the students with the chemistry of the various biochemical systems, as well as the major metabolic pathways the bio molecules are involved in. An
understanding of these biochemical systems and chemical reactions will give a better appreciation of their roles in the maintenance of life and order in the body. The course also aims to relate
biochemistry with other basic medical sciences.
The experimental work is an integral part of the course, where the biochemical processes in the cell are observed and explained on the basis of their molecular structures and reactions.
Course Intended Learning Outcomes:
1. Identify and describe the properties and chemical structure of biomolecules;
2. Describe the biochemical adaptations of these specialized fluids and tissues for the performance of their roles in the various processes of life;
3. Explain the chemistry of various body constituents ( blood and other specialized fluids; specialized tissues as bones, teeth, collagen, muscles, etc.);
4. Discuss the important mechanisms and pathways by which the different biomolecules operate as bases for the various processes of life.
5. Relate the concepts learned in understanding physiological disorders and diseases.
6. Appreciate the relation of Biochemistry to their course in understanding the true nature of diseases and its implication to health and well being.
7. Advocate appreciation and respect for human life as a result of the knowledge gained.
8. Demonstrate science-based skills and techniques in performing laboratory experiments, record and interpret results accurately and correctly;
9. Recognize the different pathological diseases arising from the disorder in the normal mechanism of the biochemical structures in the body.
10. Practice rational thinking and manifest self-respect, self-discipline, independence, honesty, and responsibility not only in further studies but also in any situation in life.
11. Apply/Use the knowledge, skills and attitudes developed during the course in coping with the different situations in life, particularly as students and generally, as members of the family
and the bigger community as well in the practice of their profession..
Time Assessment
Intended Learning Outcomes Content Teaching Learning Activities Resources
Allotment
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Time Assessment
Intended Learning Outcomes Content Teaching Learning Activities Resources
Allotment
1. Show understanding on the significance I – Introduction Class discussion on Assigned Lec: 6 Graded recitations
and relation of biochemistry to other fields Readings Graded homework
of sciences; 1. Biochemistry- Definition, Significance and Graded seatwork
Power point presentation
2. identify the important organelles of cell; Relation to other Fields of Sciences and/or boardworks
3. list down various biochemical systems 2. Review of the Cell, Organelles and Research Quiz, Unit test
that exist in the cells and identify the Functions Rubric assessment
Recitation, discussions
building blocks of each; 3. Chemical Elements and Biomolecules Evaluation of
4. correlate the composition and function of present in the Cell Homework and/or boardworks performance and
the organelles with the biochemical 4. Chemical reactions involved in the Cell Lab: 24 results of
Performance of Laboratory
system associated with it; 5. Properties of water, solutions, colloids and experiments
Experiments with pre- and post-lab
5. recognize that life is a coordination of surface tension, pH and Buffers Assessment of
discussions
several biochemical reactions; 6. Cell membrane and the transport of Laboratory
6. account for the unique properties of water nutrients: diffusion, osmosis, dialysis, Performance and
that makes it suitable as a medium for sodium potassium pump Reports
biochemical reactions; and
7. relate the principles involved in several
physical phenomena of biological
importance.

1. define carbohydrates and illustrate their II – Carbohydrates Class discussion on Assigned Lec: 8 Graded Recitation
chemical formulas and structure; Readings Graded homework
2. classify carbohydrates and discuss the 1. Definition, sources, properties and Exercises, Seatwork
properties and functions of carbohydrates; functions Power point presentation Quizzes
3. correlate the structure of 2. Classification: monosaccharides, Unit Test
carbohydrates with their function and disaccharides,polysaccharides and Recitations, seatwork and Rubric Assessment
activity; their importance boardwork Evaluation of
4. illustrate the open chain aldehyde 3. Isomerism: Fischer Structure (D & L prepared M.O.
structure of glucose (Fischer projections) isomers) and Haworth Structure (α & β models
with emphasis on D & L structures, as well anomers)
as its anomeric ring structures ( Haworth 4. Chemical Reactions of
structure) with emphasis on the α & β monosaccharides ( oxidation and
anomers; reduction, phosphorylation,
condensation)
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5. draw the structures of important sugars 5. Mechanism of the formation of the Research Assessment of
and explain some chemical reactions blue iodo-starch complex and the Lab: 12 Laboratory
that may be used to identify specific swelling of cellulose in water; Performance of Laboratory Performance and
sugar; 6. Other polysaccharides, occurrence and Experiments with pre- and post-lab Reports
6. explain the mechanism of the formation functions discussions
of the blue iodo-starch complex and 7. Health implications-related diseases or
the swelling of cellulose in water; disorders
7. name other polysaccharides and give
their occurrence and functions;
PRELIM EXAMINATION
1. define lipids, name their sources and III – Lipids Class Discussion on Assigned Lec: 5 Graded Recitation
their properties 1. Definition, sources, properties and Readings Quizzes
2. recognize the general features and functions Chapter Test
describe the physiological function of 2. Classifications: hydrolyzable and Powerpoint Presentation Assessment of
lipids and their derivatives; nonhydrolyzable; simple, Laboratory
3. classify lipids as hydrolyzable and 3. compound, and derived Research Performance and
nonhydrolyzable; give examples of 4. Biochemical importance of hydrolysable Lab: 12 Reports
each; and non hydrolysable lipids Exercise on nomenclature
4. describe the reactions undergone by 5. Chemical Reactions of lipids Homeworks, Seatworks
simple lipids and write chemical 6. Medical implications-related diseases Boardworks
equations for these; and disorders
5. give examples of compound lipids and Performance of Laboratory
explain their specific functions and Experiments with pre- and post-lab
properties; discussions
6. give examples of commonly occurring
and biochemically important steroids;
7. identify the fat soluble vitamins and
discuss their occurrence and functions
8. discuss the nature, structures and
functions of eicosanoids

1. illustrate the general structure of an IV – Proteins Class Discussion on Assigned Lec: 6 Graded recitations,
alpha amino acid, identify their 1. Definition , sources and functions Readings homeworks,
functional groups and describe their 2. Amino acids – the building blocks seatworks
importance; 3. Classification and Properties of amino Research Quizzes
2. recognize and classify amino acids acids Unit Test
based on physical and chemical 4. Chemical reaction of amino acids- Power point presentation Rubric assessment
properties, and give examples of each forming peptide bonds
class; Homeworks, Seatworks
Boardworks
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3. name the essential amino acids in man 5. Classifications of proteins based on: Performance of Laboratory Lab: 18 Assessment of
and discuss their importance; composition, structure, properties, & Experiments with pre- and post-lab Laboratory
4. show the zwitterion structure of an functions discussions Performance and
amino acid associated with its 6. Chemical reactions of proteins- Reports
zwitterion forms; denaturation, general and specific tests
5. explain isoelectric point and how an
amino acid behaves at its isoelectric
point;
6. discuss the composition, properties
and biochemical significance of
proteins;
7. illustrate how amino acids can form
peptide linkages;
8. describe the structural level of
organization in a protein;
9. explain protein denaturation, identify
what part of the structural organization
of proteins is affected, and discuss how
different agents can cause the
denaturation;

MIDTERM EXAMINATION
1. define, name the sources and function V – Enzymes Class Discussion on Assigned Lec: 4 Graded recitations,
of enzymes 1. Definition, sources and function of Readings Homeworks,
2. classify, name and give examples enzymes seatworks
of enzymes; 2. Classifications of enzymes based on: Research Quizzes
3. explain the specificity of enzyme action substrate & chemical reactions they Unit Test
4. discuss the factors that affect catalyzed Powerpoint presentation Lab: 8 Rubric Assessment
enzyme activity; 3. Specificity and Mechanism of enzyme Assessment of
5. define co-enzymes, name their action Homeworks, Seatworks Laboratory
functions and give examples of 4. Factors affecting enzyme activity Boardworks Performance and
biologically important compounds 5. Definition, sources and function of Reports
which are sources of co-enzymes; coenzymes Performance of Laboratory
6. define and differentiate the two types of 6. Types of enzyme Inhibition Experiments
enzyme inhibition and give examples; 7. Effect of denaturation on enzyme with pre- and post-lab
7. explain the effect of denaturation on activity discussions
enzyme activity; and 8. Diseases of enzymatic defects and
8. associate disorders and diseases Health implications
with learned concepts on protein
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1. define, name the sources and functions VI – Nucleic Acids Class Discussion Lec: 6 Written and Oral
of nucleic acids; reports
2. differentiate nucleosides, nucleotides 1. Definition, sources and functions Individual/Group Reports on Assigned Quizzes
and nucleic acids as to chemical 2. Chemical Composition, & Structures of Readings Chapter test
composition; DNA and RNA Rubric Assessment
3. illustrate the linkage of nitrogen base 3. Central Dogma of Protein Synthesis – Powerpoint Presentations Assessment of
and sugar in nucleosides; nucleosides Replication, Transcription and Laboratory
and phosphate components of Translation Exercises on nomenclature through Lab 9 Performance and
nucleotides; 4. Genetic Code seatworks and homeworks Reports
4. name some nucleosides and 5. Mutation of Genes
nucleotides; 6. Recombinant DNA & Polymerase Performance of Laboratory
5. explain the structural diagram of the Chain Reaction Experiments with pre- and post-lab
Watson – Crick Model of DNA 7. Medical Implications-related disorders discussions
6. explain the structural and diseases
complimentary base pairing in DNA
and RNA;
7. outline the main steps of
replication, transcription, and
translation;
8. describe the basic elements of the
genetic code; and
9. relate learned concepts with
genetic disorders and diseases

1. define metabolism, catabolism, VII. Bioenergetics Whole class or group discussion Lec: 4 Oral & written
anabolism 1. Digestion and the conversion of Food exercises
2. express in a discussion the digestion & into Energy Research Seatwork
absorption of carbohydrates, lipids and 2. ATP and Energy Production Unit test
proteins 3. Coenzymes in metabolism Powerpoint Presentation
in food 4. Citric acid Cycle
3. summarize reactions of the citric acid 5. Electron transport Chain & Oxidative Oral and written report
cycle Phosphorylation
4. describe oxidative phosphorylation- Individual/Group Reports on Assigned
electron transport chain Readings
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1. compare and contrast the metabolism VIII. Carbohydrate, Lipid, & Protein Class Discussion Lec: 9 Oral & written
of carbohydrates, lipids and proteins Metabolism exercises
2. compute the total ATP produced in 1. Glycolysis Powerpoint presentation Unit test
carbohydrates and lipid 2. Fate of Pyruvate Individual/Group Reports on Assigned Assessment of Lab
metabolism 3. ATP yield from Glucose Readings Performance and
3. relate the biosynthesis of 4. Gluconeogenesis –Cori Cycle Lab: 9 Reports
carbohydrates, lipids, and proteins with 5. Catablolism of triacylglycerols Research
the body organs 6. Ketone Bodies
7. Amino Acid Metabolism Performance of Laboratory Experiment
with pre- and post-lab discussions
Worksheet exercises
1. account for the chemistry of body IX. Body Fluids Assign topics to study prior to a lecture Lec: 3 Oral & written
fluids, how they affect the body and 1. Blood - Composition and function and whole class or group discussion exercises
their relation to body physiology; 2. Urine - Composition and function Unit tests
2. outline some of the functions and 3. Pathological composition Assigned readings/Journal report on Assessment of Lab
normal values of body fluids in 4. Water and salt balance body fluids & related diseases Performance and
humans; Reports
3. discuss the cellular components of Oral and written reports
blood and give their functions and
normal values in the human body; Performance of Laboratory Experiment Lab: 10
4. differentiate between serum and with pre- and post-lab discussions
plasma; and
5. give examples of plasma proteins and
discuss their functions in general.
1. define the different steps involved in X. Nutrition Tracing the pathways of Lec: 3 Oral & written
the digestive process; 1. Dietary calorie intake digestion exercises
2. identify the digestive fluids involved in 2. Vitamins, water and minerals Unit Test
digestion and their respective role; 3. Health related problems Oral and Written reports Assessment of Lab
3. briefly describe how carbohydrates, Performance and
lipids, and proteins are digested and Reporting about diseases/ effects of Reports
give the product of such digestions; vitamin deficiency
4. describe the absorption of digested
products Supplementary Lab. Activity: Lab: 6
5. discuss the importance of proper Extraction and Analysis of Peanut Oil
nutrition in maintaining good health
FINAL EXAMINATION
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TEXTBOOK:
Smith, J., General, Organic and Biological Chemistry. 4th ed. N.Y.: McGraw-Hill Companies, Inc. Int. ed. c2019.

REFERENCES:

Denniston, K.J., J.J. Topping and R.L. Caret General, Organic and Biochemistry. N.Y.: McGraw-Hill Companies, Inc. Int. ed. c 2007.
th
Hein, Morris; Best, Leo; Pattison, Scott; Arena, Susan. Introduction to General, Organic and Biochemistry, 8 ed. New York: John Wiley and Sons, Inc. c 2005.
nd
McMurry, John. Organic Chemistry with Biological Applications, 2 ed. Australia: Brooks/Cole c 2011.
Murray, Robert K. [Link]. Harpers Illustrated Biochemistry (Lange Medical Book). McGraw-Hill Medical. 29 edition. February 28, 2012.
Nelson, David L. and Michael M. Cox. Lehninger Principles of Biochemistry. New [Link] 2017
th
Sackheim, George I. and D.D. Lehman, Chemistry for Health Sciences. 8 ed. New Jersey: Prentice-Hall Inc. , Simon & Schuster. C 1998.
Seager, Spencer L. and Slabaugh, Michael R. Organic and Biochemistry for Today. C 2011.
st
Smith, Janice Gorzynsky. General, Organic and Biological Chemistry. 1 ed. N.Y.: McGraw-Hill Companies, Int. ed. c 2010.
nd
Sterner, Olov. Chemistry, Health and Environment. 2 . Ed. Germany: Wiley-VCH, 2010.
th
Stoker, H. Stephen. General, Organic and Biological Chemistry. 5 ed. Australia: Brooks/Cole Cengage Learning, c 2010.
Voet, Donald, et. al. Principles of Biochemistry. International Student Version Edition. John Wiley & Sons. 4th Edition. April 1, 2012
Vollhardt, K. Peter C. Organic Chemistry: Structure and Function. C. 2007.

ON-LINE REFERENCES:

Biochemistry Virtual Lab I. Retrieved from: [Link] on September 20, 2018

Carbohydrates Part 1: Simple Sugars and Fischer Projections. Retrieved from:
[Link] on September 20, 2018
Carbohydrates Part 2: Polysaccharides. Retrieved from:
[Link] on September 20, 2018
Cell Communication: Hormones and Neurotransmitters. Retrieved from:
[Link] on September 20, 2018
Cellular Respiration Part 1: Glycolysis. Retrieved from:
[Link] on September 20, 2018
Cellular Respiration Part 2: The Citric Acid Cycle. Retrieved from:
[Link] on September 20, 2018
Cellular Respiration Part 3: The Electron Transport Chain and Oxidative Phosphorylation. Retrieved from:
[Link] on September 20, 2018
DNA Replication: Copying the Molecule of Life. Retrieved from:
[Link] on September20, 2018
Enzymes: Nature's Factory Workers. Retrieved from:
[Link] on September 20, 2018
Illicit Drugs: How Do They Work? Retrieved from: [Link] September 20, 2018
Introduction to Biochemistry. Published August 24, 2016. Retrieved from: [Link] on September 20, 2018
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Lennarz, William J. Encyclopedia of Biological Chemistry. 2 edition v.1-4. 2013. Retrieved from: [Link] on
September 20, 2018
Lipids Part 1: TAGs, Fatty Acids, and Terpenes. Retrieved from:
[Link] on September 20, 2018
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Lipids Part 2: Steroids. Retrieved from:

[Link] on September 20, 2018
Mechanisms of DNA Damage and Repair. Retrieved from:
[Link] on September 20, 2018
Metabolism and ATP. Retrieved from: [Link] on
September 20, 2018
Nucleic Acids: DNA and RNA. Retrived from: [Link] on
September 20, 2018
Pharmaceutical Drugs: Inhibitors and the Nature of Disease. Retrieved from:
[Link] on September 20, 2018
Receptors: Signal Transduction and Phosphorylation Cascade. Retrived from :
[Link] on September 20, 2018
Structure Of The Cell Membrane - Active and Passive Transport. Retrieved from:
[Link] on September 20, 2018
Transcription and Translation: From DNA to Protein. Retrieved from:
[Link] on September 20, 2018
What is Cancer? Retrieved from: [Link] on September 20, 2018
Prepared by: Date: Approved by: Date:

Chairman: (Sgd.) Mrs. Perla P. Separo September 22, 2018 (Sgd.) Aleli V. Lozano September 12, 2018
Head, Physical Sciences Department

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