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Sputum Collection Guidelines for AFB

This document provides instructions for collecting a sputum sample to test for tuberculosis (TB). The best time to collect sputum is in the morning upon waking, as the TB bacteria will be more concentrated. The sample must be kept out of direct sunlight at all times, as sunlight can kill the TB bacteria and alter the test results. Proper collection involves washing hands, identifying the patient, having them cough deeply into a collection bottle, and analyzing the results within 24 hours to detect the presence of mycobacteria.

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Cristina Jefferson
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150 views2 pages

Sputum Collection Guidelines for AFB

This document provides instructions for collecting a sputum sample to test for tuberculosis (TB). The best time to collect sputum is in the morning upon waking, as the TB bacteria will be more concentrated. The sample must be kept out of direct sunlight at all times, as sunlight can kill the TB bacteria and alter the test results. Proper collection involves washing hands, identifying the patient, having them cough deeply into a collection bottle, and analyzing the results within 24 hours to detect the presence of mycobacteria.

Uploaded by

Cristina Jefferson
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Sputum Collection AFB

INDICATION:
1.To detect mycobacterial infection.
2.To diagnose TB (mycobacterium TB)
3.Monitor effectiveness of treatment.
When is the best time to collect the sputum?
The best time, but not the only time, to collect a sputum specimen is upon awakening in
the morning.
PRECAUTION:
Precautions to keep the specimen out ofsunlight at all times will be taken because
ultraviolet light kills the TB bacteria and exposure to sunlight could alter the test result.
After specimen collection the container should be shielded from sunlight during transport
into the facility.
PREPARATION:
1.Bottle for sputum collection
2. Blue colour form
3.Gloves
*Label innermost tube with patient name and date of birth before obtaining specimens
and before giving container to patient.

Procedure Rationale
1.Wash hand
2.Greet client and explain procedure
3.Provide privacy.
4. Identify client by checking ID band
5. Give bottle for sputum collection.
6. Specimen collection should be done
without the client rinsing the mouth or
brushing teeth.

7. The client should be instructed to sit or


stand and open the specimen container

8. Inhale deeply 2-3 times, breathe out hard


each time.

9. Cough deeply from the chest

10. Place the open container close to the


mouth to collect the specimen.

11. Trace lab results ( AFB within 24hours)

12. Analyse the result

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