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Report 2114239303 1

This document is an authentic check report for an individual named Mr. Anil Shelar who underwent testing for COVID-19. The test results were negative for SARS-CoV-2. The report provides details on the test such as the kit used, limitations of the test, and factors that could lead to false negatives. It was concluded that based on the negative test result and limitations described, the specimen did not detect SARS-CoV-2 but clinical symptoms should also be considered for diagnosis.

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Shubham Sane
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281 views1 page

Report 2114239303 1

This document is an authentic check report for an individual named Mr. Anil Shelar who underwent testing for COVID-19. The test results were negative for SARS-CoV-2. The report provides details on the test such as the kit used, limitations of the test, and factors that could lead to false negatives. It was concluded that based on the negative test result and limitations described, the specimen did not detect SARS-CoV-2 but clinical symptoms should also be considered for diagnosis.

Uploaded by

Shubham Sane
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Authentic Check

CID : 2114239303 SID : 177803646780 R


Name : MR.ANIL SHELAR Registered : 22-May-2021 / 11:17 E
P
Age / Gender : 36 Years / Male Collected : 22-May-2021 / 13:39 O
Dr. :- Reported : 22-May-2021 / 20:29 R
T
Reg. Location : Pune Chikhali, Gajanan Laboratory Printed : 22-May-2021 / 20:30

Real time Qualitative RT-PCR detection of 2019-nCOV RNA / COVID-19 RNA


PARAMETER RESULT
Result SARS-CoV-2: Not Detected (Negative)

Kit:Genestore SARS-CoV-2,Target gene (N1 & N2), Cutoff <37

ICMR Registration No: Andheri-Mumbai- SUBUR001, Pune-SUDIIPLPMH

Specimen: Nasopharyngeal & Oropharyngeal swab in VTM Method: Real time RT-PCR

Note:

‡ Ct value indicates the infectivity and not severity of infection.


‡ ICMR recommended kits are used for reporting. All the positive cases will be notified to ICMR for further
surveillance.
‡ Clinical correlation with patient history, radiology findings and co-infection with other virus infection is necessary to
be determined especially in cases with Borderline positive Ct values.
‡ Borderline positive cases (Ct Value >30) may give variable results on repeat testing. The possible reasons could be the
variations in kits and instruments used.

Limitations:

‡ Optimum specimen types and timing of peak viral levels during infections caused by 2019-nCOV have not been
determined. Collection of multiple specimens (Types & Time points) may be necessary in view of suspected clinical
history. The repeat specimen may be considered after a gap of 2-4 days after the collection of first specimen for
additional testing if required. (other respiratory pathogens)
‡ Negative results do not preclude SARS - CoV - 2 infection and should not be used as the sole basis for patient
management decisions.
‡ This test is a qualitative assay and does not quantify viral load. Various host factors, viral factors, variability in
sample collection / site and techniques used by the laboratories can affect Ct values. Therefore, Ct values are not an
absolute indication of viral load and should be interpreted with caution.

Factors leading to false negative RT-PCR report:

‡ Inadequate specimen collection, Poor quality of sample and non-representative sample.


‡ Sample collected too early or too late in the infection, Improper sample handling and shipment.
‡ Technical reasons- PCR Inhibitor, analytical sensitivity of kit used.
‡ Active recombination &/ mutations in target genes used for detection of SARS-CoV-2 virus.

References:

1. Diagnostic detection of 2019-nCoV by real-time RT-PCR, Berlin Jan 17th, 2020.


2. Labcorp COVID-19 RT-PCR test EUA Summary / COVID-19 RT-PCR test (laboratory corporation of America).
* Sample processed at Molecular Diagnostics Laboratory, Pune Lab, Pune Swargate
*** End Of Report ***

Dr.SHAMLA KULKARNI
MD (PATH)
MC-2463 Consultant Pathologist

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