NUEVA ECIJA UNIVERSITY OF SCIENCE AND TECHNOLOGY
COLLEGE OF ARTS AND SCIENCES
COLLEGE OF NURSING
BIOCHEMISTRY (MC2) LABORATORY
POST LABORATORY REPORT IN
CARBOHYDRATES
DATU, BEVERLY JANE L.
BSN 1-A
SEPTEMBER 24, 2019
INTRODUCTION
Carbohydrates are essential in foods as an energy source as starch is the source of human calories, a
flavoring as simple sugars is a usual sweet and as a functional ingredients like sucrose allows ice cream to be
soft in the freezer or gums thickens a low fat salad dressing. Carbohydrates are types of macronutrient found
in many foods and beverages. Most carbohydrates are naturally occurring in plant based food, such as grains.
Food manufactures also add carbohydrates to processed foods in the form of starch or added sugar.
In this experiment, carbohydrates will undergo to the different kinds of solution to test and to know what
give the positive reactor and a negative reactor between the added solutions and gained knowledge about the
structures of carbohydrates, also to know the differences between monosaccharides such as glucose, fructose,
xylose and galactose and disaccharides such as lactose, maltose, and starch. We also test the H20 and starch
if our assumptions can be corrected by this experiment.
METHODS
SELIWANOFF
BIAL’S TEST
IODINETEST
MOLISCH TEST
5ml solutions + add 2 10 drops TS 2.5 ml seliwanoff 3ml BR + 1ml solutions
drop of molisch reagent
5 Mix
ml BS + 0.5 ml sugar
throughly 5ml
10 drops iodine &BR &5boil
mix 50mix
drop solutions and mg boil
G +&1ml DW
dilute + nbutanol
boil & cool
Reddish-violet ring 8 drops of solution & boil
Dark reddish-yellow Red color 1ml NABlue-green
& boil color
Brick-red ppt Green, yellow or red Crystal ppt
BARFOED’S TEST BENEDICT TEST MUCIC TEST
Figures 1-7: General Procedures of all Carbohydrates
POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae
RESULTS
In this section, it represents the following tables and image documents from the experiment that we
have conducted and verification that we are all present and participating the experiment.
Table 1. pH Determination Table 2.1 Buffer Action
SUBSTANCES PH ACIDIC BASIC NEUT
LEVEL RAL
BEER
5 ✔
MILK 5 ✔
SPRITE 3 ✔
DISTILLED 7 ✔
WATER
VINEGAR 2 ✔
0.1 M HCI 2 ✔
Table 2.2
TES
PH
T LEVEL PH
TEST
OF LEVELS
TUB
COMBINE TUBE OF
D SOLUTI BASIC
E ACIDI BASI NEUTRA
SOLUTIO NO. ONS ACIDIC NEUTRAL
NO. N C C L 1 7 ✔
9 ✔
1 2 7 ✔
7 ✔
2 3 2 ✔
3 3 ✔
4 13 ✔
4 10 ✔
5 4 ✔
5 4 ✔
7 8 ✔
7 9 ✔
8 9 ✔
8 8 ✔
POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae
CONCLUSION
It is concluded that the experiment was successful in using all the test such as benedict test, iodine
test, molisch test, seliwanoff test, bial’s test, barfoed’s test and mucic test. Benedict test is used to detect the
presence of reducing sugar such as glucose, fructose and lactose. All monosaccharides are reducing sugar,
they all have a free reactive carbonyl group. Some disaccharides have expose carbonyl group and are also
reducing sugar. Lactose and maltose which is disaccharides also called reducing sugar as it exposes the
carbonyl group. Other disaccharides such as sucrose and starch are non-reducing sugar and will not react with
Benedict test. Next, we use iodine test to test for the presence of starch. Starch is a type of polysaccharides
carbohydrates which is made up of amylose and amylopectin. Starch give positive result in iodine test as the
rest gives an negative result because iodine is only for the test of starch. Molisch test gives an positive results
except for H20. H20 is gives a negative reactor to all carbohydrates because the bond between the oxygen
and hydrogen atoms in sugar gives the oxygen a slight negative charge and the hydrogen a slight positive
charge. The polar water molecules attract the negative and positive areas on the polar molecules which make
it dissolve in the water. Barfoed’s test give an positive to all the monosaccharides sugars such as glucose,
fructose, xylose and galactose because it is only test for monosaccharides. Seliwanoff test is a test only for
ketoses that is result for a positive to the fructose and sucrose, the rest gives a negative results. Mucic test is a
test for only galactose that means galactose is only gives an positive and the rest is negative and last is bial’s
test is for pentoses that means the only example of pentoses is xylose or arabinose. This experiments was
construct to see how the following carbohydrates react with the other substances and solutions that gives us
have more knowledge and to be aware of how many sugars in our daily lives do we digest.
REFERENCE:
Study Moose. (2016). Carbohydrates Lab Report. Retrieved from https://studymoose.com/carbohydrates-lab-
report-essay
POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae
POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae
