Tissue Culture Lab Project Report
Capacity: 3 million plantlets per year
LAND
The tissue culture laboratory should be preferably located in a moderated climate condition having uninterrupted supply
of water and power. The tissue culture operations have to be carried out under controlled conditions of temperature.
Extreme climatic condition adds to the cost of maintenance
Approximate 5 acres land should be adequate for setting up a TC unit with the above capacity.
PROJECT COST
Sr.No. Particulars Cost (In Lakhs)
1 Fixed assets 163.95
2 Technology know how 15.00
3 Working Capital (3 months) 17.28
Total 196.23
SUBSIDIES
Various Central and State Government departments have framed financial schemes
1) The Department of Agriculture and Cooperation
There is a provision for assistance of up to Rs. 21 lakhs and Rs. 10 lakhs for setting up tissue culture units in
public and private sector respectively subject to a maximum of 20% of the project cost.
2) Agricultural and Processed food products Export Development Authority (APEDA)
3) National Horticulture Board (NHB)
For setting up of a new tissue culture lab there is a provision for back-ended capital subsidy not exceeding 20% of
the project cost with a maximum limit of Rs. 25 lakh per project.
4) Department of Biotechnology (DBT)
5) Small Farmers Agri business Consortium (SFAC)
SFAC under the Ministry of Agriculture give soft loans up to 50 lakhs for setting up of small tissue culture labs
by cooperative societies formed by small scale farmers.
MARKET
Demand for tissue cultured plantlets is growing rapidly. India, with its low-cost skilled labour as well as scientific
manpower (both of which are essential for tissue culture) has a natural advantage. Additional favourable factors are the
wide range of plant biodiversity in the country and favorable tropical climate (which enables greenhouses with low energy
consumption).
The potential for the domestic market is enormous and by conservative estimates it is around Rs. 200 crores with an
annual growth rate of 20%. There are more than 70 established commercial tissue culture units. Their production capacity
ranges between 0.5 million to 10 million plants per annum with an aggregate production capacity of about 200 million
plantlets per year. The protocols have either been developed in-house or transferred through the various research
institutions and universities engaged in development of the protocols through support of the Department of Biotechnology
(DBT) Currently, the focus of the companies is mainly banana, floriculture, sugarcane and potato.
With increasing awareness about the advantages of tissue culture raised plants in improving yield and quality, their
domestic consumption is also increasing optimistically. The major consumers of tissue culture raised plants are the State
Agriculture Department, Agri Export Zones (AEZs), State agencies such as Spice Board, sugar industry and private
farmers. The paper industry, medicinal plant industry and State Forest Departments are using tissue culture raised plants
in a limited scale. Also, a number of progressive farmers and nurseries in the states are the major consumers of Tissue
culture plants particularly for flowers, banana, sugarcane and medicinal plants.
CONSTRUCTION
The building of about 8800 sq.ft includes class 1000 clean rooms and areas with comfort AC for laboratory, growth rooms
and office space. The following facilities would be required in the building.
a) Storage room for chemicals
b) Washing and Media preparation room
c) Sterilization room
d) Inoculation room
e) Culture room
The total cost is estimated at Rs. 35.20 lakhs @ Rs. 400/sq.ft.
Green house
A green house of 7500 sq.ft. and a shade house of 80,000 sq.ft. have been assumed at a cost of Rs. 22.00 lakhs and 8.00
lakhs (total Rs. 30 lakhs) respectively. The greenhouse should be provided with heating equipment, fans and cooling
systems.
EQUIPMENT
Major equipment and instruments required for the plant are as follows.
Autoclave
Laminar air flow cabinet
Equipment for sterilization
Electronic weighing balance
Water distillation apparatus
Air handling units
Refrigerator
Air conditioners
Stereomicroscope
Digital pH meter
Shelves / racks
Green house material