Tube Guide and
Recommended Order of Draw*
BD Vacutainer®
BD Life Sciences - Preanalytical Systems
Cap Tube Special
Cat. No. Determinations
Colour Type Instructions
Optimal volumes:
Blood Aerobic followed by anaerobic- if insufficient blood for both culture bottles
Adult: 8 - 10ml
Cultures use aerobic bottle only
Paediatric: 1 - 3ml
Cat. No.
Mix
363095 / KFK119 Sodium Fill to frosted minimum fill line
Draw Volume Citrate
D-Dimer, APTT, INR, coagulation studies
Paediatric: KFK117 1.8ml
3-4
Times
2.7ml
Cat. No. For cold agglutinins, Donath- Mix
367837 / KFK168 Landsteiner sample must be
Draw Volume
Serum As requested by laboratories, cold agglutinins, Donath- Landsteiner transported at 37oC. Contact 5-6
Times
6ml blood transfusion 20339 in advance
Cat. No. Biochemistry/Haematology: Troponin, FSH, LH, Testosterone, Oestradiol, Cryoglobulin samples must be
Progesterone, Prolactin, Cortisol, Growth Hormone, IGF1, Tumour Markers, transported at 37°C. Contact
367954 / KFK114 Iron Studies, Ferritin, B12, Folate, EPO, Haptoglobin, Glandular Fever, Lithium Immunology on 25995 for Mix
SST™ II
Advance
Immunology: immunoglobulins, protein electrophoresis, autoantibodies, advice. 6
Draw Volume complement, allergy, cryoglobulins, serum free light chains, functional Functional complement samples Times
complement (CH50, AP50 and C1 esterase inhibitor) must arrive in the Immunology
5ml Microbiology: bacterial serology, viral serology, Helicobacter pylori lab with 4h.
Cat. No. Biochemistry: Sodium, Potassium, Urea, Creatinine, Chloride, Bicarbonate, CRP, Mix
365055 Bilirubin, ALT, ALP, GGT, Total Protein, Albumin, Calcium, Phosphate, Magnesium,
Draw Volume
Barricor™
LDH, CK, Urate, Amylase, Cholesterol, Triglyceride, Paracetamol, Salicylate, 8-10
Times
3ml Anticonvulsants, Digoxin, Ethanol, TSH, fT4, fT3
Cat. No. Mix
Biochemistry: Sodium, Potassium, Urea, Creatinine, Chloride, Bicarbonate, 8-10
367373 / KFK130 CRP, Bilirubin, ALT, ALP, GGT, Total Protein, Albumin, Calcium, Phosphate, Lymphocyte proliferation Times
PST™ II Magnesium, LDH, CK, Urate, Amylase, Cholesterol, Triglyceride, Paracetamol, must first be discussed with
Draw Volume Salicylate, Anticonvulsants, Digoxin, Ethanol, TSH, fT4, fT3, Immunology on 25995 Mix
Immunology: TB Quantiferon, TB ELISpot, Lymphocyte proliferation 8-10
3ml Times
Cat. No. Mix
Biochemistry / Haematology: Full Blood Count, ESR, RBC Folate, HbA1c, Sickle 8-10
367839 / KFK171 Cell (Hb Electrophoresis), Cyclosporin, Tacrolimus, PTH Times
Transfusion: Group and Save, Crossmatch
EDTA Paediatric: KFK276 2ml
Immunology: Lymphocyte Markers, CD4
Draw Volume Microbiology: Viral & Bacterial PCR viral load
4ml
Cat. No.
368920 / KFK250 Fluoride
Glucose, Lactate Paediatric: KFK250 2ml
Draw Volume Oxalate
2ml
Determinations and Special Instructions contained within this guide have IMPORTANT MIXING GUIDELINES
been provided by the named institute and are not BD recommendations or All BD Vacutainer® tubes require immediate mixing following collection.
instructions for the BD products described. Please consult your organisation’s Insufficient mixing can result in inaccurate test results and the need to
guidelines or contact BD should you have any questions. re-draw. Correct mixing technique is to gently invert (180o and back) each
*Clinical and Laboratory Standards Institute (CLSI) Guidelines GP41-Ed7 (formerly H3-A6, 6th Edition)
tube the recommended number of times shown on the right hand side of
the table.
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