206-97445

Jul. 2011

For Shimadzu UV-Visible

Spectrophotometer
UV Performance Validation Software
INSTRUCTION MANUAL

Read the instruction manual thoroughly before you use the product.
Keep this instruction manual for future reference.
This page is intentionally left blank.
 Preface

Read this manual thoroughly before using the product.

Thank you for purchasing this product, the UV Performance Validation Software for the Shimadzu
UV-Visible Spectrophotometer (hereafter referred to as "this software").
This manual describes how to use this software. Read the manual thoroughly before using this
software and use this software in accordance with the manual's instructions. Keep this manual for
future reference.
This manual assumes that you are familiar with the basic operations of the Windows operating
system. For Windows operations, refer to the instruction manual and other documents included with
the relevant product.

„ IMPORTANT
• If the user or usage location of this software changes, be sure this manual is always kept with the
product.
• If this manual is lost or damaged, contact your Shimadzu representative immediately.
• To ensure safe usage of this software, contact your Shimadzu representative for installation,
adjustment, or re-installation after moving the product to a different site.

„ COPYRIGHT
• Information in this manual is subject to change without notice and does not represent a commitment
on the part of the vendor.
• Any errors or omissions which may have occurred in this manual despite the utmost care taken in its
production will be corrected as soon as possible, but not necessarily immediately upon detection.
• Copyright of this manual is owned by Shimadzu Corporation. Reproduction or duplication of its
content, in whole or part, without permission of the copyright holder is strictly prohibited.
• Windows is a registered trademark of Microsoft Corporation in the United States and/or other
countries. All other brand names and product names are trademarks or registered trademarks of their
respective companies. The TM and ® symbols are omitted in this manual.
• Replacement parts for this product will be available for a period of seven (7) years after the
discontinuation of the product. Thereafter, such parts may cease to be available. Note, however, that
the availability of parts not manufactured by Shimadzu shall be determined by the relevant
manufacturers.
© 2011 Shimadzu Corporation. All rights reserved.
Original version is approved in English.

UV Performance Validation Software i

Preface

Notations Used in This Manual

In this manual, warnings are indicated using the following conventions:

Notation Description
Indicates a potentially hazardous situation which, if not avoided, could
WARNING result in serious injury or possibly death.

The following pictorial symbols and conventions are used in this manual with the following
meanings.

Notation Description
Emphasizes additional information that is provided to ensure the
proper use of this product.

Indicates information provided to improve product performance.

Indicates the location of related information in the instruction manuals.

Indicates text or expressions that appear in the window, such as

Text in square brackets [ ]
buttons, menu items, setting options, window titles, and icons.
Text in quotation marks " " Indicates entered numerical values, text, and keyboard key names.

In this instruction manual, window operations are explained by using the following descriptive rules.

Notation Description
Click Means selecting an object and pressing the left mouse button once.
Double-click Means selecting an object and pressing the left mouse button twice.
Means dragging an object to the target location and then releasing the
Drag and drop
left mouse button.

ii UV Performance Validation Software

 Safety Instructions

Safety Instructions
To ensure safe operation of the product, read these Safety Instructions carefully before use.
Observe all of the WARNINGS described in this manual. They are extremely important for safety.

„Operation Precautions
Note that the hard disk data of your PC may be damaged by an unforeseeable accident.
Be sure to back up your important data to protect it from such accidents.
Do not measure or place any explosive, flammable, or ignitable substances inside the product.

WARNING
When handling any toxic, deleterious, or volatile samples, take appropriate safety measures
by wearing adequate working clothes, protective goggles, masks, and gloves in accordance
with the Material Safety Data Sheet (MSDS) guidelines created by the relevant reagent
manufacturers.
Failure to observe this may result in an accident.
If an accident involving chemicals occurs, take relevant first-aid and emergency measures according
to the instructions provided by the relevant organizations.

UV Performance Validation Software iii

Preface

Warranty
Shimadzu provides the following warranty for this product.

1. Period: Please contact your Shimadzu representative for information about the
period of this warranty.
2. Description: If a product/part failure occurs for reasons attributable to Shimadzu during
the warranty period, Shimadzu will repair or replace the product/part free
of charge. However, in the case of products which are usually available on
the market only for a short time, such as personal computers and their
peripherals/parts, Shimadzu may not be able to provide identical
replacement products.
3. Limitation of 1. In no event will Shimadzu be liable for any lost revenue, profit or data,
or for special, indirect, consequential, incidental or punitive damages,
Liability: however caused regardless of the theory of liability, arising out of or
related to the use of or inability to use the product, even if Shimadzu has
been advised of the possibility of such damage.
2. In no event will Shimadzu's liability to you, whether in contract, tort
(including negligence), or otherwise, exceed the amount you paid for
the product.

4. Exceptions: Failures caused by the following are excluded from the warranty, even if
they occur during the warranty period.
1. Improper product handling
2. Repairs or modifications performed by parties other than Shimadzu or
Shimadzu designated companies
3. Product use in combination with hardware or software other than that
designated by Shimadzu
4. Computer viruses leading to device failures and damage to data and
software, including the product's basic software
5. Power failures, including power outages and sudden voltage drops,
leading to device failures and damage to data and software, including
the product's basic software
6. Turning OFF the product without following the proper shutdown
procedure leading to device failures and damage to data and software,
including the product's basic software
7. Reasons unrelated to the product itself
8. Product use in harsh environments, such as those subject to high
temperatures or humidity levels, corrosive gases, or strong vibrations
9. Fires, earthquakes, or any other act of nature, contamination by
radioactive or hazardous substances, or any other force majeure event,
including wars, riots, and crimes
10. Product movement or transportation after installation
11. Consumable items
Note: Recording media such as floppy disks and CD-ROMs are
considered consumable items.
* If there is a document such as a warranty provided with the product, or there is a
separate contract agreed upon that includes warranty conditions, the provisions of
those documents shall apply.

iv UV Performance Validation Software

 Contents

Preface............................................................................................... i
Notations Used in This Manual......................................................................................................ii
Safety Instructions ........................................................................................................................ iii
Warranty .......................................................................................................................................iv

1 Installation
1.1 Details of Components ........................................................................................1
1.2 Installing Software ...............................................................................................1
1.3 Starting Software .................................................................................................7
1.4 Configuring Communication Port.........................................................................8
1.5 Establishing Communication with Instrument ......................................................9
1.6 Setting Password...............................................................................................10
1.7 Exiting Software.................................................................................................10

2 Software Overview
2.1 Intended Instrument Types ................................................................................11
2.2 Configuration of Main Window...........................................................................13
2.2.1 File Property Pane ................................................................................................. 14
2.2.2 Batch of Test Pane................................................................................................. 15
2.2.3 System Status Pane............................................................................................... 16

2.3 Checking Result During Test .............................................................................17

2.4 Test Using Multi-Cell..........................................................................................18
2.5 File Types ..........................................................................................................19
2.6 Print Layout........................................................................................................20
2.7 Other Features...................................................................................................22
2.7.1 Restricting Functions Based on Account ............................................................... 22
2.7.2 Convenient Functions for Test Conditions ............................................................. 23
2.7.3 Automatic Standby for Test.................................................................................... 25

3 Test Overview
3.1 Initialization Results (Record) ............................................................................27
3.2 Semi-Automatic Test Items................................................................................28

UV Performance Validation Software v

Contents

3.3 Automatic Test Items......................................................................................... 31

3.4 Pass/Fail Decision ............................................................................................. 33
3.4.1 Decision Digit Count............................................................................................... 33

4 Example of Software Operation

4.1 Test Flow ........................................................................................................... 35
4.2 Test Conditions.................................................................................................. 36
4.2.1 Configuring Test Conditions ................................................................................... 37
4.2.2 Saving Test Conditions .......................................................................................... 41
4.2.3 Loading Test Conditions ........................................................................................ 43

4.3 Performing a Test .............................................................................................. 44

4.3.1 Checking Test Tools............................................................................................... 44
4.3.2 Entering Information About the Test Results File................................................... 45
4.3.3 Starting/Aborting a Test ......................................................................................... 47

4.4 Checking Test Results in Process..................................................................... 48

4.4.1 Method for Checking Test Results ......................................................................... 48
4.4.2 Method for Checking Result File ............................................................................ 49

4.5 Printing .............................................................................................................. 51

5 Test Items
5.1 Details of Test Items.......................................................................................... 53
5.2 Test Operations ................................................................................................. 54
5.2.1 Wavelength Accuracy............................................................................................. 54
5.2.2 Wavelength Repeatability....................................................................................... 55
5.2.3 Photometric Accuracy ............................................................................................ 56
5.2.4 Photometric Repeatability ...................................................................................... 57
5.2.5 Resolution (Absorbance Ratio) .............................................................................. 58
5.2.6 Stray Light .............................................................................................................. 59

6 Menu Commands
6.1 [File] Menu......................................................................................................... 61
6.1.1 System Settings ..................................................................................................... 63

6.2 [View] Menu....................................................................................................... 64

6.2.1 Setting Graph Drawing ........................................................................................... 64

6.3 [Test] Menu ....................................................................................................... 65

6.4 [Instrument] Menu.............................................................................................. 66
6.5 [Security] Menu.................................................................................................. 66

vi UV Performance Validation Software

 Contents

6.6 [Help] Menu .......................................................................................................67

6.6.1 Help ........................................................................................................................ 68

7 Troubleshooting
7.1 Test Result Fails ................................................................................................69
7.1.1 "Failed" Appears in Initialization Setting Record .................................................... 69
7.1.2 Wavelength Accuracy Failed.................................................................................. 69
7.1.3 Wavelength Repeatability Failed............................................................................ 70
7.1.4 Photometric Accuracy Failed ................................................................................. 71
7.1.5 Photometric Repeatability Failed ........................................................................... 71
7.1.6 Resolution Failed ................................................................................................... 72
7.1.7 Stray Light Failed ................................................................................................... 72
7.1.8 Noise Level Failed.................................................................................................. 73
7.1.9 Baseline Flatness Failed ........................................................................................ 73
7.1.10 Photometric Drift (Baseline Stability) Failed ........................................................... 73

7.2 Error Messages .................................................................................................74

7.2.1 Error Message List ................................................................................................. 74
7.2.2 Error Code List ....................................................................................................... 76

7.3 Others ................................................................................................................77

8 Appendixes
8.1 Recommended Value List..................................................................................79
8.2 Test Method Using UVProbe .............................................................................81
8.2.1 Testing Resolution Using Benzene Vapor ............................................................. 81
8.2.2 Testing Stray Light Using Mesh Filter .................................................................... 82

8.3 Reference Data..................................................................................................84

8.3.1 What is Stray Light? ............................................................................................... 84
8.3.2 Influence by Hydrogen Emission Line .................................................................... 85

Index ............................................................................................... 87

UV Performance Validation Software vii

Contents

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viii UV Performance Validation Software

1 1 Installation

This chapter explains the installation process relevant to this software (software and instrument
setups, starting and exiting software, etc.).
1
1.1 Details of Components 1
Configuration (main components) of the software package (206-28340-91)
Table 1-1
1
No.
1
Part Name
UV Performance Validation Software (Install CD)
Part No.
206-28341-91
Qty
1
1
UV Performance Validation Software Instruction Manual
2
(this instruction manual)
206-97445 1
1
1.2 Installing Software 1
The following explains the installation procedure for this software.
1
This software is compatible with Windows 7, Windows Vista, and Windows XP operating systems (All
Edition 32-bit). Check the operating system running on your personal computer (hereafter called a "PC") 1
before installing this software.

1
1 Turn on the power to the PC. After Windows has started, insert the installation CD of
this software into the CD-ROM drive.
1
In the Windows XP operating system, the setup program starts automatically and the window
shown in step 4 appears. If the setup program does not start automatically, take the following steps
to start it manually. 1
This example assumes that drive "E" is assigned to the CD-ROM drive. For "E:\", substitute the

1
correct drive assigned to the CD-ROM drive of your PC.

1 Click (start), and then click [Run...].

The [Run] window appears.
2 Enter "E:\autorun.exe", and click [OK]. 1
1
1
1
UV Performance Validation Software 1
1 Installation

2 When the [AutoPlay] window appears, click [Run autorun.exe].

Fig.1-1

If the [AutoPlay] window does not appear, take the following steps to start the setup program. This
example assumes that drive "E" is assigned to the CD-ROM drive. For "E:\", substitute the correct
drive assigned to the CD-ROM drive of your PC.

1 Click (Start).
2 Enter "E:\AutoRun", and press the "Enter" key.

Fig.1-2

3 When the [User Account Control] window appears, click [Yes].

Fig.1-3

2 UV Performance Validation Software

 1.2 Installing Software

4 Read the precautions and click [Next].

Fig.1-4

The following window appears if this software is found on your PC during a software upgrade.
Click [Remove] to uninstall the software.

Fig.1-5

UV Performance Validation Software 3

1 Installation

5 Enter the appropriate information for [User Name] and [Organization], then click
[Next].

Fig.1-6

You may enter up to 50 characters for the [User Name] and up to 80 characters for the
[Organization].

6 Configure the instrument information.

3
Fig.1-7

1 Select [InstrumentType] of the instrument to be connected.

2 Enter [InstrumentName] and [SerialNumber].
3 Check that the settings and the entered data are correct, and click [Next].

In UV-2600/2700 series, the serial number is loaded when they are connected. Therefore the
serial number field is disabled. Also note that the [InstrumentType] selection cannot be
changed after installation.
Note that for UV-1600/2400PC/2500PC series, the information selected and entered for
[InstrumentType] and [SerialNumber] cannot be changed after installation.

4 UV Performance Validation Software

 1.2 Installing Software

7 Check the destination folder to install the software, and click [Next].

Fig.1-8

To change the destination folder, take the following steps:

1. Click [Change].
2. Select or enter the destination folder to install the software, and click [OK].

Fig.1-9
Folder securities for Windows XP or later versions are stricter than those of earlier versions. Therefore,
creating new sub folders or saving files in the destination folder may be restricted. To change the
destination folder used for installation, check the folder security settings in advance.

UV Performance Validation Software 5

1 Installation

8 When you are ready to begin installation, click [Install].

Fig.1-10

9 Click [Finish].

Fig.1-11
Installation is then completed.

6 UV Performance Validation Software

 1.3 Starting Software

1.3 Starting Software

1 Double-click the shortcut icon on your desktop.

2 Select the account and enter the password, then click [OK].
1

Fig.1-12

For information about accounts, see "2.7.1 Restricting Functions Based on Account".

Logging in is possible immediately after installation without entering a password.

The software starts.

Immediately after installation, the software initially starts with a default (recommended) test item and
other conditions ( "8.1 Recommended Value List"). Subsequently, the software will start with the
conditions that you configured during the last operation.

Fig.1-13

UV Performance Validation Software 7

1 Installation

1.4 Configuring Communication Port

Configure the communication port in the [Instrument Settings] window.
In this window, you can change the instrument name specified during installation, and check
information about the instrument currently connected.

You can only connect to the type of instruments that you configured during installation.
Do not connect to other instruments even if the type of the other instruments is the same as the ones you
configured. Each instrument has its own unique serial number.

See "2.1 Intended Instrument Types".

The following shows how to configure the communication port.

If your PC has two or more communication ports, check the number of the port to which the
communication cable is connected.

A UV-2600/2700 is connected via USB, but it uses the communication port inside the PC.
Refer to the "UV-2600/2700 Instruction Manual" and check the connected communication port number
using the device manager.

1 Click [Configure] from the [Instrument] menu.

Fig.1-14

2 From the [Communications Port] list, select the port to which the instrument's
communication cable is connected.

Fig.1-15

3 Click [OK].

8 UV Performance Validation Software

 1.5 Establishing Communication with Instrument

1.5 Establishing Communication with Instrument

You can only establish communication with the type of instruments that you configured during installation.
Do not connect to other instruments even if the type of the other instruments is the same as the ones you
configured. Each instrument has its own unique serial number. 1
See "2.1 Intended Instrument Types".

To simply view previous test results, communication with the instrument does not need to be established.

1 Turn on the power switch of the instrument (spectrophotometer).

2 Click [Connect].

Fig.1-16
The Initialization Setting window appears.

3 After the initialization settings are completed, click [OK].

Fig.1-17
When communication with the instrument has been established, the [Connect] button on the tool button
bar changes to [Disconnect], and [Start] is enabled (turns green).

Fig.1-18

UV Performance Validation Software 9

1 Installation

1.6 Setting Password

Set the password required for logging in.
Note that the administrator account is required to set or change passwords.

1 Click [Administrative] from the [Security] menu.

2 Select the account to be changed, and enter a new password (enter it twice for
confirmation).
You may enter up to 30 alphanumeric characters. The password is case sensitive.

Fig.1-19

3 Click [Change] to confirm the change.

4 Click [Close].
The [Administrative] window closes.

1.7 Exiting Software

1 Click [Disconnect].

Fig.1-20

Before turning off the power switch of the instrument, be sure to disconnect communication.

2 Click [Exit] from the [File] menu.

10 UV Performance Validation Software

2 2 Software Overview

This software tests the performance of the spectrophotometer using predefined procedures
(measuring parameters) and stores the results. This process is referred to as "validation" in the
software and in the Installation Manual.
2
This software controls the spectrophotometer from a personal computer (PC), collects and processes
data, then makes a pass or fail decision based on predefined acceptance criteria. 2
This software only checks the performance of a spectrophotometer. It will not operate correctly if optional
accessories are attached to the spectrophotometer.
2
2
2.1 Intended Instrument Types
This software can control and test the following instrument types. 2
• UV-1600 series (UV-1601/UV-1601PC/UV-1650PC)
• UV-2400PC series (UV-2401PC/UV-2450) 2
• UV-2500PC series (UV-2501PC/UV-2550)
• UV-2600 series 2
• UV-2700 series

Note that items that can be tested and measuring parameters that can be configured vary depending on
2
the instrument type. Therefore, this software can only control (test) a single instrument type after
installation. 2
Be sure to only use this software for a single instrument under a single (PC) environment.
The type and serial number of the instrument to be tested must be specified when the software is
being installed.
2
See "1.2 Installing Software". 2
2
2
2
2
2
UV Performance Validation Software 11
2 Software Overview

• The instrument type cannot be changed after installation. If you want to change the instrument type,
reinstall the software. Note that if the instrument type is changed, the test condition file currently used
can no longer be used.
• For the UV-1600/2400PC/2500PC series, the serial number or the instrument type cannot be changed
after installation. A different instrument of the same instrument type can be connected, but the serial
number specified during installation will be recorded in the test result file. As a result, the instrument
information contained in the result file will be different from that of the actual instrument. Therefore, do
not use this software for a different instrument.
• For the UV-2600/2700 series, the instrument serial number is stored in the internal memory. When the
instrument is connected, this software loads the stored information, and thus a different instrument can
be connected and controlled (tested) as long as it is the same instrument type as the one initially
configured. As the instrument name is set as part of the instrument information in the software
( "6.4 [Instrument] Menu"), the name must be changed each time you change instruments. If you
forget to change the setting, obtained test results may become invalid. Therefore, it is recommended that
you only use this software for a single instrument under a single environment (PC).

12 UV Performance Validation Software

 2.2 Configuration of Main Window

2.2 Configuration of Main Window

4
3
2

5
6

Fig.2-1

Table 2-1

No. Name Description Reference

1 Menu bar Select the function of this software from each menu item. Chapter 6
2 Tool button bar Displays tool buttons of frequently used functions. Chapter 6
Enter information about a test results file automatically
3 File Property pane saved after measurement. If a test results file is currently Section 2.2.1
open, information about that file is displayed.
Displays the test items and respective results.
4 Batch of Test pane For each test item, you can specify "Tested" or "Not Section 2.2.2
Tested".
Displays the remaining test time and progress of the test
5 System Status pane Section 2.2.3
(event log).
Displays data currently tested, or the test results of the
item selected in the Batch of Test pane.

6 Result pane See "2.3 Checking Result During Test". Section 5.1
Details to be displayed vary depending on the test item.
For more details, see the help of the software for each test
item.

UV Performance Validation Software 13

2 Software Overview

2.2.1 File Property Pane

Enter information about the test results file.
Before starting a test, you must specify the folder to save the file to, the result file name, and the
analyst name.

See "4.3.2 Entering Information About the Test Results File".

The settings for the destination folder and the result file name are saved in the test condition file.

Fig.2-2

Table 2-2

No. Display Description

Specify the folder to save the test results file to.
Click to browse for a folder.

1 Folder for Saving The default path is "C:\UVPerfval-Data\ResultFiles".

You may create a new folder in the dialog box, but is may take a few seconds to
create the new folder after you click the "new folder" button.
Enter the name of the test results file.
If a file with the same name exists in the destination folder, the date and time is
added to the end of the file name (as shown in the following example) indicating
2 Test Results File when the test started.
Example: If the test started on September 9, 2011 at 10:30 AM and 56:21
seconds,
"resultdata2011090910305621.pvr".
Enter the name of operator responsible for the test.
3 Analyst
You may enter up to 20 alphanumeric characters.

14 UV Performance Validation Software

 2.2 Configuration of Main Window

2.2.2 Batch of Test Pane

1 2 3 5
7

4
6
2
Fig.2-3

Table 2-3

No. Display Description

1 Select All Checks all test item boxes.
2 Clear All Unchecks all test item boxes.
Lists reference filter and other devices required for testing.
3 Test Tools
See "4.3.1 Checking Test Tools".
Items with checked boxes will be tested.
This setting is not saved in the test condition file.
4 Checkboxes

See "2.7.2 Convenient Functions for Test Conditions".

Lists test items selected in the test condition setting.
You can check or uncheck a box by clicking it.
During a test or when a result is displayed, item rows and characters are
highlighted in different colors to indicate the state of progress or results.

See "4.2 Test Conditions".

5 Batch of Test(s) Row colors
Orange: Indicates that the item has been selected to check the test result. The
result of the selected test item is displayed in the Result pane.
Green: Indicates that the item is currently being tested.
Aqua: Indicates that the item has been tested, aborted, or skipped.
Character colors
Red: Indicates that the test failed.
Displays the overall pass/fail decision of the result for each test item.
Passed: All tests for the selected test item had satisfactory results.
Failed: A test for one or more selected test items had unsatisfactory
results or the test was aborted (or untested).
6 Result Example for "WL Accuracy (Deuterium Discharge Lamp)": If the
result of wavelength 486.0 nm is determined as "Passed", while
testing for wavelength 656.1 nm was aborted and determined as
"Failed", then the overall result becomes "Failed".
Not excuted: The item was not tested.
Displays the name of the condition file currently loaded.
7 Condition file name If a file was created and has not yet been saved, a file name is not displayed in
this field.

UV Performance Validation Software 15

2 Software Overview

2.2.3 System Status Pane

Displays the total test time

before testing starts.

Displays the remaining test

2 time during the test.

Fig.2-4

Table 2-4

No. Display Description

Progress of Current Displays the progress of the current test for the selected test item using the
1
Test progress bar with the remaining time indicator.
Displays the time (total test time) required for completing all tests for the
2 Overall Progress selected test items. The progress bar with the remaining time indicator appears
when the test starts.
The test status is displayed together with the remaining test time during a test.
The content is saved in a result file as an event log. When the result file is loaded,
the log list is displayed at the same position.
3 Event Log
However, the event log cannot be printed.

For information about the result file, see "2.5 File Types".

• The "total test time" or "remaining test time" does not include standby time (i.e., the wait time required
for the instrument to stabilize) during a test.
• The "total test time" includes the time required for testing all items with checked boxes in the Batch of
Test pane.
• Both the "total test time" and "remaining test time" are only intended for use as a rough indicator. For
some items, the discrepancy may be a few minutes.

16 UV Performance Validation Software

 2.3 Checking Result During Test

2.3 Checking Result During Test

When a test has started, the result of the current test is displayed in real time in a window (called
View Test window). You can also display the result of a test item already completed (called View
Result window).
The windows can be switched using [View Test] or [Results] on the tool button bar or the [View]
menu. ( "6.2 [View] Menu")

2
The View Result window appears automatically when the test has been completed. To start another test,
configure test conditions or load the existing test conditions.

For the procedure to switch the windows, see "4.4 Checking Test Results in Process".

Displays the data currently

measured in real time.

View Test window

Click the desired Click [View Test].

test item.

View Result window

The test item for which the result is

displayed appears in orange.

Displays the results or graphs for the

test item that is highlighted in orange in
the [Batch of Test(s)] field.

Fig.2-5

UV Performance Validation Software 17

2 Software Overview

2.4 Test Using Multi-Cell

If a test requires tools (reference filter, etc.), use a multi-cell (accessory) to set the tools automatically.
To use a multi-cell, select the multi-cell type in the [Edit Test Conditions] window.

1 3

Fig.2-6

Table 2-5

No. Display Description

The analyst in charge of the test must change test tools according to the message
1 None
displayed during a test.
2 6 Cell Select this to connect the 6-cell multi-cell sample compartment (accessory).*
Select this to connect the 6-cell electronic temperature control cell positioner
3 CPS-240
(accessory).*

* For the part number required when ordering, see "3.2 Semi-Automatic Test Items".

When you use the multi-cell, a message appears indicating the position to insert test tools when you
start a test for each semi-automatic test item.
Follow the message to insert the test tools, and click [OK] to start the test.

Fig.2-7

18 UV Performance Validation Software

 2.5 File Types

2.5 File Types

This software can create two types of files.

„ Test results file (*.pvr)

When the test processes are completed, a result file is created automatically and saved in the specified
folder.
Included in the result file are measurement and decision results, waveform data (spectrum and time
course), measuring parameters, and an event log, which can be checked or printed after being loaded
2
using this software.

For the method used to check test results, see "4.4 Checking Test Results in Process".

„ Parameter file (*.pvm)

A test condition file includes the following information:
• Items to be tested (test items) and their order
• Detailed conditions for respective test items (wavelength, acceptance criteria, etc.)
• Information related to test tools (such as verified value, expiration date, control number of
reference filter)
• Names of the destination folder and file where results are saved*
* The names of the destination folder and file are those entered in the file property pane in the Main window
( "2.2.1 File Property Pane") when you save the condition file.

For more details about configuring, saving, or loading a condition file, see "4.2 Test Conditions".

At the time of installation, the "UVPerfval-Data" folder is created in the install drive as the initial
destination folder for the result file and the condition file. This folder will not be removed even when the
software is uninstalled.

UV Performance Validation Software 19

2 Software Overview

2.6 Print Layout

The layout to print test results can be selected from "Print data with graph" or "Print data without
graph". The supported paper size and orientation is A4 portrait. If you print data in letter size, you
must change the report template files.
• Print data with graph: Common area + Test result details (with graph)
• Print data without graph: Common area + Test result details (without graph)

See "6.1.1 System Settings".

„ Common area
Information related to the test is printed regardless of the configured print layout.

1
2

Fig.2-8

Table 2-6

No. Display Description

Prints the time and date when all test processes are completed or aborted, and the
1 Test Date
result file is saved.
2 Software Version Prints the version number of this software used for the test.
Instrument Prints information about the instrument (instrument type, instrument name, and
3
Information serial number).
Prints the firmware version of the instrument used for the test when a UV-2600/
4 F/W Version
2700 series unit is used for the test.
Prints information (analyst name and comment) that is entered when the test is
5 File Information
started.
Prints test items and respective test results (displayed in the Batch of Test pane).
6 Test Results
For the Batch of Test pane, see "2.2.2 Batch of Test Pane".

20 UV Performance Validation Software

 2.6 Print Layout

„ Test result details

Graphs and measuring parameters are printed in addition to the results for each test item.

For details to be printed, see "5.1 Details of Test Items" or the help of the software explaining details of
each test item.

1 2
3
2

Fig.2-9

Table 2-7

No. Display Description

1 Test Item Name Prints the name of test item.
Measurement Prints the content shown on the [Measurement Parameters] tab in the Main
2
Parameters window.
Prints the information (control number and expiration date) of reference filter or
Test Tool other test tools used as reference of the test.
3
Information
The test tool name is included in the test item name for display and printing.
4 Test Results Prints the content shown on the Result tab in the Main window.
Prints a graph for an item that has obtained spectrum data or time course data
during the test.
5 Graph
Scaling and other settings of the graph are the same as it is shown in the Main
window.

UV Performance Validation Software 21

2 Software Overview

2.7 Other Features

2.7.1 Restricting Functions Based on Account
This software has three accounts, and four functions to which access can be restricted for each
account. Access to those functions by logged in accounts can be restricted by assigning the functions
to respective accounts.
The following table shows the available functions and their default settings for each account.
For more details about the functions, see references indicated in table 2-8.
Table 2-8

Function Account
Item Reference System administrator Test administrator Operator
Change Instrument
Section 6.4 { (Cannot change) × ×
Configuration
Edit Test Conditions Section 6.3 { (Cannot change) { ×
Change System Settings Section 6.1.1 { (Cannot change) { ×
Select Test Items to
Section 2.2.2 { (Cannot change) { {
Execute*

* Indicates the function that allows the user to select test items not in the test conditions but in the Batch of Test pane in the
Main window by checking test item boxes.

1 Click [Administrative] from the [Security] menu.

2 Click the [Changed Rights] tab to select the account and the function.

Fig.2-10

1 Select the account to be edited.

2 Check the box of the function to be used.
3 Click [Change] to confirm the change.

3 Click [Close].
The [Administrative] window closes.

22 UV Performance Validation Software

 2.7 Other Features

2.7.2 Convenient Functions for Test Conditions

„ Registering frequently used test conditions

This software can register up to three frequently used test condition files.
For example, test conditions for periodic inspection (such as test items and the acceptance criteria)
can be registered as a "frequently used condition" when the conditions are saved as a file. The
registered condition can be easily selected and loaded using a tool button in the Main window.

For more details about this function, see "4.2 Test Conditions".
2

Fig.2-11

„ Listing test conditions

The current test conditions can be confirmed and printed in a list.
Use this function to make sure that the loaded test condition file has no errors. This function can be
used even if you are not authorized to edit the conditions or view the Edit window.
From the [Tests] menu, click [View Conditions] to open the Preview window and view the test
conditions.

Fig.2-12

UV Performance Validation Software 23

2 Software Overview

„ Selecting "Tested" or "Not Tested" (Selecting Batch of Test)

You can select items to be tested without changing test conditions.
For example, if the test fails for only one item as a result of testing multiple items, you can load the
same test conditions to only test the failed item again.

The "Tested" or "Not Tested" setting is not saved in the test condition file.

1 Click [Clear All].

This unchecks all boxes of test items.

Fig.2-13

2 Check the box of the item to be tested.

Fig.2-14

24 UV Performance Validation Software

 2.7 Other Features

2.7.3 Automatic Standby for Test

It is recommended that some items be tested in stable temperature and lamp conditions of the
instrument.

See "3.3 Automatic Test Items".

If the recommended amount of time for instrument stabilization* has not passed before a new test
starts, the new test is put on standby until the instrument stabilization time has elapsed, after which
the test starts automatically.
It is also possible to forcibly restart the test during the instrument stabilization time.
2
* The following shows the time required for the instrument to be stabilize (recommended).
• UV-1600/2400PC/2500PC series: 2 hours
• UV-2600/2700 series: 1 hour

Fig.2-15

Table 2-9

No. Display Description

Indicates the remaining time until the stabilizing time ends.
1 Standby time
When the stabilizing time ends, the test restarts.
2 Execution Click this to abort standby and restart the test.

The method to count stabilizing time differs depending on the instrument type.
• UV-1600/2400PC/2500PC series
The stabilizing time is counted after this software completes the initialization settings of the
spectrophotometer.

See "3.1 Initialization Results (Record)".

Note that if another type of software configured the initialization settings of the spectrophotometer before
this software connects with the spectrophotometer to start the test, the time will not be able to be counted
accurately.
• UV-2600/2700 series
The continuous lighting time of the lamp is counted. Because the spectrophotometer stores the time even
before being connected to this software, this software can obtain the time and add it to the count.

UV Performance Validation Software 25

2 Software Overview

This page is intentionally left blank.

26 UV Performance Validation Software

3 3 Test Overview

This chapter provides an overview of items this software can test.

The items this software can test can be categorized into the following three groups. 3
• Initialization results (record)
• Semi-automatic test items
3
• Automatic test items

3
3.1 Initialization Results (Record)
UV-1600/UV-2400PC/UV-2500PC series
3
Outputs the results of the spectrophotometer's initialization and self-diagnostic.
To test the items, be sure to turn on the power switch of the spectrophotometer before establishing 3
communication with this software.

See "1.5 Establishing Communication with Instrument".

3

UV-2600/UV-2700 series
3
Outputs the latest initialization and diagnosis results stored in the instrument.
3
3
3
3
3
3
3
3
3
UV Performance Validation Software 27
3 Test Overview

3.2 Semi-Automatic Test Items

These test items require a reference filter or other test tools for testing.
If two or more test tools are used for testing, an operator will be required to insert or remove the test
tools according to messages displayed during the test.
Use an accessory such as the 6-Cell Multi Cell Sample Compartment or CPS-240A to change the test
tools automatically during the test.

See "2.4 Test Using Multi-Cell".

For more details about each item, see "5.1 Details of Test Items" or the help of the software.

Table 3-1

Test Item Description

Measures the absorbance or transmittance of a transmittance calibration filter*, and
determines, based on the criterion, whether or not to accept the error between the
measured value and the calibration reference value (verified value) attached to the
filter.
You can register several filters with different transmittance to test with all or any of
the filters. For more accurate testing, it is recommended that you set an approximate
(about ± 3 °C) sample (filter) temperature specified in the calibration certificate of
Photometric Accuracy the calibration filter to be used.
The 6-Cell Electronic Temperature Control Cell Holder (CPS-240A) can be used to
control the filter temperature during the test.

See "2.4 Test Using Multi-Cell".

To set acceptance criteria values, add accuracy of the calibration filter specified in
the certificate.
Measures the photometric value (absorbance or transmittance) using a transmittance
calibration filter* at a wavelength set arbitrarily. Repeats the measurement three
times in total to obtain the average value, and determines, based on the criterion,
Photometric
whether or not to accept the deviation of each measured value from the average
Repeatability
value.
As is the case with photometric accuracy, the calibration filter can be added or
changed.

28 UV Performance Validation Software

 3.2 Semi-Automatic Test Items

Test Item Description

Measures the relative stray light. The value is obtained by: {(Sum of light intensity
at wavelengths other than the set wavelength)/(Light intensity output from the
spectrometer at the set wavelength)} × 100.
The stray light value at the following wavelengths can be determined:
• 198 nm - When potassium chloride solution (12 g/l) is used (Only for UV-2600/
2700)
• 220 nm - When sodium iodide solution (10 g/l) is used
• 340 nm - When sodium nitrite solution (50 g/l) is used
Stray Light • 370 nm - When sodium nitrite solution (50 g/l) is used (Only for UV-2600/2700)

Absorbance error caused by stray light is described in "8.3.1 What is Stray Light?".
Refer to this section when you specify the acceptance criterion value.
3
For UV-2500PC series, this software cannot check the instrument specifications.

For more information, see "8.2.2 Testing Stray Light Using Mesh Filter".
Measures the absorbance spectrum of 0.02 %V/V toluene solution in hexane
("toluene/hexane solution") to obtain the absorbance ratio of the maximum (peak)
Resolution value in the vicinity of 269 nm and the minimum (valley) value in the vicinity of
(Absorbance Ratio) 266 nm, and then determines whether or not to accept the value based on the
criterion.
This test uses the method specified in European Pharmacopoeia to check resolution.
Measures a wavelength calibration filter* to determine, based on the criterion,
whether or not to accept the error between the measured value and the reference
WL Accuracy
wavelength attached to the filter. To set the acceptance criterion value, add accuracy
(uncertainty) of the calibration filter specified in the certificate.
Measures an arbitrarily selected absorption peak three times using a wavelength
calibration filter* to obtain the average value, and determines, based on the criterion,
WL Repeatability whether or not to accept the deviation of the measured value from the average value.
As is the case with accuracy of the wavelength, the calibration filter can be added or
changed.

* Supplied by the National Institute of Standards and Technology (NIST) or the Japan Quality Assurance Organization
(JQA) by being attached to glass optical filters or solution filters for calibrating transmittance (absorbance) of the
spectrophotometer and calibrating wavelength values.

UV Performance Validation Software 29

3 Test Overview

Table 3-2 lists the test tools used for semi-automatic test.
Table 3-2 Test Tools Used for Semi-Automatic Test

Test Item No. Part Name Supply Source or P/N

Photometric Accuracy/ NIST and the Japan Quality
1 Transmittance calibration filter*1
Repeatability Assurance Organization (JQA)
WL Accuracy/ NIST and the Japan Quality
2 Wavelength calibration filter*1
Repeatability Assurance Organization (JQA)
Reagent used to check resolution,
specified in European
Resolution 3 Pharmacopoeia*2 Relevant reagent distributors
(0.02 percent V/V solution of
toluene R in hexane R)
4 Sodium iodide solution (10 g/l) Relevant reagent distributors
5 Sodium nitrite solution (50 g/l) Relevant reagent distributors
6 Potassium chloride solution (12 g/l) Relevant reagent distributors
Stray Light
7 Quartz 10 mm square cell 200-34442
8 Shutter block 202-30338
9 Mesh filter 206-82299-92

*1 Use the filter calibrated at the same slit widths (bandwidths) as used for actual test. Table 3-3 lists the slit widths for testing
with this software.

Table 3-3 Slit Widths for "WL Accuracy" and "Photometric Accuracy"

UV-2400PC/2500PC
Test Item UV-1600 Series UV-2600/2700 Series
Series
WL Accuracy 2.0 nm 1.0 nm 1.0 nm
Photometric Accuracy 2.0 nm 2.0 nm 2.0 nm

*2 Hexane solution is also required for a blank solution.

As item 7 is used as cells for items 4 to 6, the same quantity is required as the number of reagents to be
tested.
To test a stray light lower than 0.001 % for the UV-2600/2700 series, item 9 is required.

See "5.2.6 Stray Light".

Table 3-4 Multi-Cell Available for Semi-Automatic Test

Part Name (Type) P/N

6-Cell Multi Cell Sample Compartment 206-69160-01
6-Cell Electronic Temperature Control Cell Positioner (CPS-240A) 206-23760-91

30 UV Performance Validation Software

 3.3 Automatic Test Items

3.3 Automatic Test Items

These items can be tested automatically from measurement to pass/fail decision once the test starts.
Table 3-5

Test Item Description

Measures the time change in the vicinity of absorbance 0 Abs at an
arbitrarily set wavelength, and obtains the movement width of absorbance
per minute, which is defined as the noise level (P-P, i.e., Peak-to-Peak).
Also obtains the RMS value*1 from the measured data per minute. Then
Noise Level
determines, based on the criterion, whether or not to accept those two
values. It is recommended that you test for this item in a state where the
temperature in the instrument becomes stable*2 when a certain time has
3
passed after power-on.
Measures the time change in the vicinity of absorbance 0 Abs at an
arbitrarily set wavelength to obtain the changing rate per hour, which is
defined as drift (baseline stability). Then determines, based on the criterion,
whether or not to accept the value.
Photometric Drift (Baseline Checking drift is susceptible to the fluctuation of operating and
Stability) environmental temperature.
It is recommended that you test for this item in a state where the temperature
in the instrument becomes stable*2 when a certain time has passed after
power-on. An environment where the ambient temperature fluctuates only
within 2 °C/h is also recommended.
Performs baseline correction while no samples are in the sample
compartment and wavelength scanning immediately after that to obtain the
bending rate of spectrum, which is defined as baseline flatness, and then
determines, based on the criterion, whether or not to accept the value. It is
Baseline Flatness
recommended that you test for this item in a state where the temperature in
the instrument becomes stable*2 when a certain time has passed after power-
on. An environment where the ambient temperature fluctuates only within
2 °C/h is also recommended.
Measures the emission line (656.1 nm) from the deuterium lamp used as a
Resolution light source of the instrument to obtain half width of the spectral waveform
(Bandwidth) (spectral band width), which is defined as resolution, and then determines,
based on the criterion, whether or not to accept the value.
Measures the emission line from the deuterium lamp used as a light source
WL Accuracy (Deuterium of the instrument, and determines, based on the criterion, whether or not to
Discharge Lamp) accept the error between the measured value and the emission line
wavelength.
Measures the emission line from the deuterium lamp used as a light source
WL Repeatability (Deuterium of the instrument three times to obtain the average reading value of
Discharge Lamp) wavelength, and then determines, based on the criterion, whether or not to
accept the deviation of each measured value from the average value.
This test item is for UV-2600/2700 series.
WL Accuracy*3 (Mercury Measures the emission line from the optional low-pressure mercury lamp,
Lamp) and determines, based on the criterion, whether or not to accept the error
between the measured value and the emission line wavelength.

UV Performance Validation Software 31

3 Test Overview

Test Item Description

This test item is for UV-2600/2700 series.
Measures the emission line from the optional low-pressure mercury lamp
WL Repeatability*3 (Mercury
three times to obtain the average reading value of wavelength, and then
Lamp)
determines, based on the criterion, whether or not to accept the deviation of
each measured value from the average value.
*1 The following equation is used to obtain the value.
n
∑ (A – A
i=1
i m )2

RMS value = --------------------------------------

N
Ai: Absorbance, Am: Average value of absorbance, N: Number of data items
*2 See "2.7.3 Automatic Standby for Test".
*3 The following lamp unit (accessory) is required.

Part Name P/N

Mercury Lamp Unit 206-28300-41

32 UV Performance Validation Software

 3.4 Pass/Fail Decision

3.4 Pass/Fail Decision

3.4.1 Decision Digit Count
A measurement result is displayed using the same number of digits (after the decimal point) as
defined for acceptance criteria.
If a criterion has three digits after the decimal point, then the fourth digit of the measured value is
rounded (a value of 5 or higher is rounded up while a value of 4 or lower is rounded down). The
rounded (displayed) value is used to determine whether it passes or fails the criterion.

For some test items, the digits can be changed. 3

In that case, the number of digits after the decimal point of the criterion is the decision digit count.

Fig.3-1

UV Performance Validation Software 33

3 Test Overview

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34 UV Performance Validation Software

4 4 Example of Software
Operation
This chapter shows the operating procedure required for testing, and explains the operation of
software using a simple example.
4
4.1 Test Flow 4
The following shows the operation flow to test performance of the instrument using this software.
4
1 Start software ( Section 1.3)
4
4
Use the saved test conditions (for test items or acceptance criteria).

2 Configure test conditions ( Section 4.2.1) Load test conditions ( Section 4.2.3)
4
3 Start test ( Section 4.3.3) 4
4 Check results ( Section 4.4)
4

5 Print results ( Section 4.5)

4
Selected from recommended test items, this chapter only explains the test procedure for the following
4
items.
• WL Accuracy (Deuterium Discharge Lamp) 4
• Baseline Flatness
• Photometric Accuracy 4
Operate the software according to the procedure shown in the above flow in order from 1 to 5. See
the section of this manual relevant to each step as shown in the boxes in the flow. 4
4
4
4
UV Performance Validation Software 35
4 Example of Software Operation

4.2 Test Conditions

To test the instrument, performance specifications of the instrument to be tested (test items),
acceptance criteria based on which pass/fail is determined, information of tools to be used, and other
conditions required for testing ("test conditions") must be configured.
This section explains the procedure to configure test conditions and the method to save and load
them.

Before testing for semi-automatic test items, add test tools to be used, and enter the relevant information
(control number, expiration data, wavelength, etc.).

See "4.2.1 Configuring Test Conditions".

The following shows the [Edit Test Conditions] window.

Fig.4-1

36 UV Performance Validation Software

 4.2 Test Conditions

Table 4-1

No. Display Description

Saves the configured test conditions in a file.
1 Parameter File
See "4.2.2 Saving Test Conditions".
Displays items that can be tested with this software.
Items with checked boxes will be tested.
2 Test Items List The items are tested in order from top to bottom of the list.

See "4.2.1 Configuring Test Conditions".

Opens the window for configuring detailed conditions (such as registering test
tools and setting acceptance criteria) for the test item highlighted (selected) in the
Detail list.
4
3

See "4.2.1 Configuring Test Conditions".

Moves up the order of the test item highlighted (selected) in the list by one.
4 Move Buttons The order for test items in the same group (automatic or semi-automatic) can be
changed freely, but you cannot change the order between the different groups.
When two or more test tools are used for semi-automatic test, a multi-cell
(accessory) can be used to change the tools automatically.
5 Multi Cell

See "2.4 Test Using Multi-Cell".

4.2.1 Configuring Test Conditions

1 Click [New] from the [File] menu.

2 In the test item list in the [Edit Test Conditions] window, select items to be tested ([WL
Accuracy (Deuterium Discharge Lamp)], [Baseline Flatness], and [Photometric
Accuracy]).

1 Make sure that the [Semi-Automatic Test Items] box is checked, and click .
The display of semi-automatic test items expands.

Fig.4-2

UV Performance Validation Software 37

4 Example of Software Operation

2 Check only the [10% ND Filter] box under [Photometric Accuracy] in the semi-automatic
test items.

Items in the test item list with checked boxes will be tested. You can check or uncheck a box by
clicking it.

3, 4

Fig.4-3
3 Make sure that the [Automatic Test Items] box is checked, and click .
The display of automatic test items expands.
4 Check only the [WL Accuracy (Deuterium Discharge lamp)] and [Baseline Flatness] boxes
in the automatic test items.

3 Configure the information of the test tool used for [10% ND filter] under [Photometric
Accuracy].

Fig.4-4

1 Click (select) [10% ND filter] under [Photometric Accuracy].

2 Click [Detail].

38 UV Performance Validation Software

 4.2 Test Conditions

The [Photometric Accuracy] window appears.

5 4

Fig.4-5
3
4
Make sure that [10% ND filter] is selected (highlighted).
4 Select [Absorbance].
5 Click [Edit].
The [Reference material] window appears.

Fig.4-6
6 Enter the information (Serial Number, Wavelength, Standard value, etc.) of the test tool to
be used.

Only the columns with the light blue cells in the wavelength list can be directly edited.
7 Click [OK].
The [Reference material] window closes.

UV Performance Validation Software 39

4 Example of Software Operation

4 Configure detailed conditions (acceptance criteria, etc.) of [10% ND filter] under

[Photometric Accuracy].

2
Fig.4-7

1 Enter the acceptance criterion (“Tolerance”) for each wavelength directly.

2 Click [OK].
The [Photometric Accuracy] window closes.

The operating example in this section assumes that recommended detailed conditions (such as
acceptance criteria) are used for [WL Accuracy (Deuterium Discharge Lamp)] and [Baseline
Flatness]. To change the conditions, configure them by referring to help topics that can be
viewed from the Detailed Condition Setting window.
Example: Help topic for [WL Accuracy (Deuterium Discharge Lamp)].

Fig.4-8

40 UV Performance Validation Software

 4.2 Test Conditions

4.2.2 Saving Test Conditions

The test conditions configured (created) in "4.2.1 Configuring Test Conditions" can be saved as a test
condition file.
This section explains the operations for saving the conditions in the [Edit Test Conditions] window.

The conditions can also be saved from the [File] menu.

See "6.1 [File] Menu".

1 Click [Save].

4
Fig.4-9

2 Open the destination folder, enter the file name, and click [Save].

Fig.4-10

UV Performance Validation Software 41

4 Example of Software Operation

„ Registering the conditions in the test conditions list

A maximum of three test condition file frequently used can be registered in the test conditions list.
The test conditions list is displayed in [Load] in the Main window, enabling you to load a test
condition file with a simple operation.

See "4.2.3 Loading Test Conditions".

To register a condition file in the test conditions list, configure the following setting when saving the
test conditions.

1 Check the [Register in test condition list] box.

2 Select [(Conditions 1)], [(Conditions 2)], or [(Conditions 3)].

Fig.4-11

42 UV Performance Validation Software

 4.2 Test Conditions

4.2.3 Loading Test Conditions

This section explains the operation for loading the test conditions saved in "4.2.2 Saving Test
Conditions".

1 Click [Load] on the tool button bar.

The [Open-Test Conditions File] window appears.

Fig.4-12

2 Click (select) the desired condition file, and click [Open].

4

Fig.4-13

„ Loading the conditions from the test conditions list

You can also load the registered test conditions from the list.

See "„ Registering the conditions in the test conditions list".

1 Click T next to [Load].

Fig.4-14

UV Performance Validation Software 43

4 Example of Software Operation

2 In the test conditions list, click the desired test condition file.

Fig.4-15

4.3 Performing a Test

This section explains the operations before starting a test.

4.3.1 Checking Test Tools

Some test items require apparatuses such as reference filters and solutions as well as reagents (test
tools) for testing.
Add test tools to be used or edit reference values in the Detailed Condition Setting window for each
test item.

See "4.2.1 Configuring Test Conditions". For test tools required for each test item, see "3.2 Semi-
Automatic Test Items" and "3.3 Automatic Test Items".

Before starting a test, make sure that all the required test tools are ready and usable (check the
expiration date of each test tool).

If the expiration date of a tool has passed, the test cannot be started.

1 Click [Test Tools] in the Batch of Test pane.

The [List of Test Tools] window appears.

Fig.4-16

44 UV Performance Validation Software

 4.3 Performing a Test

2 Check the test tools required for testing and their expiration dates.

Fig.4-17

4.3.2 Entering Information About the Test Results File

When all test processes have been completed, the test results are saved (as a test results file)
automatically. Enter the information required for the test (file name, destination folder, etc.) before
starting the test.

The "Folder for Saving" and the "Test Results file" are saved in the test condition file. If necessary, enter
file information before saving the condition file.

1 Click for the destination folder.

The [Browse For Folder] window appears.

Fig.4-18

UV Performance Validation Software 45

4 Example of Software Operation

2 Select the folder where the test results file will be saved, and click [OK].

Fig.4-19

3 Enter [Test Results File] and [Analyst].

Fig.4-20

You can enter a comment by clicking .

46 UV Performance Validation Software

 4.3 Performing a Test

4.3.3 Starting/Aborting a Test

„ Starting a test

1 Click [Start].

Fig.4-21
The test starts and progresses in the order shown in the Batch of Test list.
If any operations such as inserting or removing test tools are required, a message appears accordingly.
Follow the message to take the required action. 4
For more information, see "5.2 Test Operations".

„ Aborting (terminating) a test

Select whether to abort (terminate) all test processes or to only abort and skip the test item currently
in progress.

1 Click [Stop].

Fig.4-22

UV Performance Validation Software 47

4 Example of Software Operation

2 Select the method for aborting the test.

To abort and skip the test item currently in progress, check the [Abort current test and start next test.]
box. To terminate the test, check the [Abort all tests.] box.

Abort and skip the test

item currently in progress

Terminate the test

Fig.4-23

While the [Abort Test] window appears, the test continues and is not aborted.

3 Click [OK].

4.4 Checking Test Results in Process

4.4.1 Method for Checking Test Results
Testing data (spectrum, time course curve, etc.) is displayed in real time during a test (in the View
Test window).
When all test processes have been completed, a window (View Result window) displays the test
results for each item.
This section explains the procedure for switching windows.

„ Viewing test results

1 In the Batch of Test pane, click the item you want to check.
The background color turns orange.

Fig.4-24

48 UV Performance Validation Software

 4.4 Checking Test Results in Process

2 Click the result tab for which you want to check details.

Fig.4-25

„ Returning to the window (View Test window) displaying the test results
in real time 4
1 Click [View Test].

Fig.4-26

4.4.2 Method for Checking Result File

„ Opening the result file

1 From the [File] menu, click [Open Test Result].

The [Open-Test Result File] window appears.

2 Select (click) the desired result file, and click [Open].

The test results are displayed.

For the method to check the test results, see "„ Viewing test results" in "4.4.1 Method for Checking
Test Results".

UV Performance Validation Software 49

4 Example of Software Operation

„ Checking file information

1 Click [Properties] from the [File] menu.

Fig.4-27

50 UV Performance Validation Software

 4.5 Printing

4.5 Printing

1 Click [Print] from the [File] menu.

2 Check the output destination (printer name) and other information, and click [OK].

Fig.4-28

Fig.4-29

UV Performance Validation Software 51

4 Example of Software Operation

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52 UV Performance Validation Software

5 5 Test Items

5.1 Details of Test Items 5

For more details about each test item, see the following.
• Recommended test items and values for each spectrophotometer: "8.1 Recommended Value List" 5
• Overview of each test item: "Chaper 3 Test Overview"
• Test conditions (registering and editing test tools, method to configure test, etc.) for each test
item: Help
5
Help topics can be viewed from the Detailed Condition Setting window. 5
Example: Help topic for Photometric Accuracy
5
5
5
5
5
5
5
5
5
5
Fig.5-1

The help menu can also be opened from the tool button.
5
See "6.6 [Help] Menu". 5
UV Performance Validation Software 53
5 Test Items

5.2 Test Operations

Because automatic testing does not use test tools, all processes, from the start to end of each test and
the transition to the next test, are carried out automatically.

• This software automatically turns the lamps on and off before and after a test. Be sure to turn on the
power switch of the "Mercury Lamp Unit" before starting a test. For the position of the power switch,
refer to the Instruction Manual of the "Mercury Lamp Unit".
• An optional "Mercury Lamp Unit" is required for "WL Accuracy (Mercury Lamp)" and "WL
Repeatability (Mercury Lamp)". Before starting the test, install the lamp unit onto the spectrophotometer
according to the instructions in the included Instruction Manual.

See "3.3 Automatic Test Items".

This section explains the behavior and operation of the software (instrument) during the test of semi-
automatic items. For display of test results or other subject, see the relevant help topics.

5.2.1 Wavelength Accuracy

The test is performed for each test tool (wavelength calibration filter, etc.).
When you use an optional multi-cell holder ( "2.4 Test Using Multi-Cell"), insert multiple test
tools into the cell position indicated by "Operation message 1" below. Then you can use those test
tools without changing them during the test.

Instrument (software) behavior Operation procedure

Displays operation message 1

1 From the cell holder, remove tools
used for the pretest. (Make sure
that nothing is placed in the cell
holder.)

Baseline correction
2 Close the sample compartment
cover, and click [OK].
The Message window closes.

Displays operation message 2

3 Insert test tools into the cell holder.

4 Close the sample compartment

cover, and click [OK].
Wavelength measurement The Message window closes.

Peak pick Processes from measurement to pass/fail

decision are performed for each wavelength.

Pass/Fail decision

54 UV Performance Validation Software

 5.2 Test Operations

5.2.2 Wavelength Repeatability

The test is performed for each test tool (wavelength calibration filter, etc.).
When you use an optional multi-cell holder ( "2.4 Test Using Multi-Cell"), insert multiple test
tools into the cell position indicated by "Operation message 1" below. Then you can use those test
tools without changing them during the test.

Instrument (software) behavior Operation procedure

Displays operation message 1

1 From the cell holder, remove tools
used for the pretest. (Make sure
that nothing is placed in the cell
holder.)

2 Close the sample compartment

cover, and click [OK].
Close the Message window.
Baseline correction
5
Displays operation message 2
3 Insert test tools into the cell holder.

4 Close the sample compartment

cover, and click [OK].
Close the Message window.

Repeats wavelength measurement

(peak pick) three times
Processes from measurement to pass/fail
decision are performed for each wavelength.

Pass/Fail decision

UV Performance Validation Software 55

5 Test Items

5.2.3 Photometric Accuracy

The test is performed for each test tool (transmittance calibration filter, etc.).
When you use an optional multi-cell holder ( "2.4 Test Using Multi-Cell"), insert multiple test
tools into the cell position indicated by "Operation message 1" below. Then you can use those test
tools without changing them during the test.

Instrument (software) behavior Operation procedure

Displays operation message 1

1 From the cell holder, remove tools
used for the pretest. (Make sure
that nothing is placed in the cell
holder.)

2 Close the sample compartment

cover, and click [OK].
Close the Message window.

Baseline correction (or Auto Zero)

Measures the blank value at wavelength

Displays operation message 2

3 Insert test tools into the cell holder.

Measures absorbance (transmittance)

4 Close the sample compartment
cover, and click [OK].
at wavelength Close the Message window.

Pass/Fail decision

56 UV Performance Validation Software

 5.2 Test Operations

5.2.4 Photometric Repeatability

The test is performed for each test tool (transmittance calibration filter, etc.).
When you use an optional multi-cell holder ( "2.4 Test Using Multi-Cell"), insert multiple test
tools into the cell position indicated by "Operation message 1" below. Then you can use those test
tools without changing them during the test.

When you use a multi-cell holder, test tools cannot be inserted or removed during repeatability
measurement.

Instrument (software) behavior Operation procedure

Displays operation message 1

1 From the cell holder, remove tools
used for the pretest. (Make sure
that nothing is placed in the cell
holder.)

2 Close the sample compartment 5

cover, and click [OK].
Close the Message window.

Baseline correction (or Auto Zero)

Displays operation message 2

3 Insert test tools into the cell holder.

4 Close the sample compartment

cover, and click [OK].
Close the Message window.

Measures absorbance (transmittance)

at wavelength
5 Remove test tools in the cell
holder.

Repeats behavior three times in total.

6 Repeat steps 3 to 5 two more
times.

Pass/Fail decision

UV Performance Validation Software 57

5 Test Items

5.2.5 Resolution (Absorbance Ratio)

When you use an optional multi-cell holder ( "2.4 Test Using Multi-Cell"), insert multiple test
tools into the cell position indicated by "Operation message 1" below. Then you can use those test
tools without changing them during the test.

Instrument (software) behavior Operation procedure

Displays operation message 1

1 From the cell holder, remove tools
used for the pretest. (Make sure
that nothing is placed in the cell
holder.)

2 Close the sample compartment

cover, and click [OK].
Close the Message window.
Baseline correction

Displays operation message 2

3 Insert toluene/hexane solution into
the cell holder on the sample side,
and hexane solution (blank) into
the cell holder on the reference
side.

4 Close the sample compartment

cover, and click [OK].
Spectrum measurement Close the Message window.

Pass/Fail decision

58 UV Performance Validation Software

 5.2 Test Operations

5.2.6 Stray Light

The test is performed for each solution (filter) for testing stray light.
When you use an optional multi-cell holder ( "2.4 Test Using Multi-Cell"), insert test tools into
the cell position indicated by "Operation message 1" below. Then you can use the multiple solutions
(filters) for stray lights without changing them during the test.

Instrument (software) behavior Operation procedure

Displays operation message 1

1 From the cell holder, remove tools
used for the pretest. (Make sure
that nothing is placed in the cell
holder.)

2 Close the sample compartment

cover, and click [OK].
Close the Message window.

Auto Zero 5

Displays operation message 2

3 Insert a shutter block into the cell
holder on the sample side.

4 Close the sample compartment

cover, and click [OK].
Close the Message window.

Measures 0 %T

Displays operation message 3

5 Instead of the shutter block, insert
a solution (filter) for testing stray
light.

6 Close the sample compartment

cover, and click [OK].
Close the Message window.

Measures transmittance at wavelength

Pass/Fail decision

UV Performance Validation Software 59

5 Test Items

„ Checking stray light using a mesh filter

UV-2700 series is a low stray light instrument as it has a specification of maximum 0.00005 %T at
220 nm.
To check the performance at the recommended 0.001 %T or higher level, the cell holder on the
reference side must be left blank for testing. However, to check the stay light value at lower than
0.001 %T within the instrument specification range (by setting the number of decision digits to 4 to 5
after the decimal point), the light on the reference side must be dimmed and the measurement range
must be enlarged.
In that case, insert a test tool into the cell holder on the sample side and a mesh filter
( "3.2 Semi-Automatic Test Items") into the cell holder on the reference side at "Operation
message 1" and start the test.

• For UV-1600/2400PC/2600 series, no mesh filters are required as the value level is out of the instrument
specification range.
• UV-2500PC series is also an instrument with low stray light, but this software cannot test a stray light
value lower than 0.001 %T.

To check a stray light value lower than 0.001 %T within the instrument specification range, see
"8.2.2 Testing Stray Light Using Mesh Filter" to use "UVProbe Software" for testing.

60 UV Performance Validation Software

6 6 Menu Commands

6.1 [File] Menu 6

Table 6-1

Command Description Tool Button Reference 6

Use this command when creating new test
conditions.
The recommended test items and conditions are 6
set, and the [Edit Test Conditions] window
New — Section 4.2
appears.
6
For information about initial conditions, see "8.1
Recommended Value List".
The [Open-Test Result File] window appears.
6
Open Test Result Select the folder where result files are stored, and — Section 4.4.2
open the desired test results file. 6
Load Test Conditions (The following menu appears)
(Conditions 1)
(Conditions 2)
Displays the name of registered condition file and
path to the file. You can click a file name to load
6
its condition settings.
(Conditions 3)
Section 4.2.3 6
The [Open-Test Conditions File] window appears.
Load Test
Conditions
Select the folder where test condition files are
stored, and open the desired test condition file. 6
Save Test Conditions Saves the test conditions currently configured. — Section 4.2.2
Sets warning (when to show the message) of 6
System Settings expiration of a test tool, and changes print layout — Section 6.1.1

6
(with or without graph).
Displays the print preview window.

6
Print preview — (None)
6
Fig.6-1
6
6
6
UV Performance Validation Software 61
6 Menu Commands

Command Description Tool Button Reference

Prints the results currently loaded (or the results of
test completed).
The [Print] window appears where you can select
Print Section 4.5
the printer or change settings. The supported paper
size and orientation is A4 portrait.
( "6.1.1 System Settings")
Displays the information of test results (file)
Properties — Section 4.4.2
currently loaded.
Exit Exits this software. — Section 1.7

62 UV Performance Validation Software

 6.1 [File] Menu

6.1.1 System Settings

Fig.6-2

Table 6-2

No. Display Description

If this box is checked, a message appears before the validity of a reference
1
Check expiration of material expires. The message appears before the test starts.
reference material The time the message starts to appear can be selected from [Before 10 days], 6
[Before 20 days], and [Before 30 days].
For a print layout, options to print with or without a graph can be selected.

For more details about print layout, see "2.6 Print Layout".
This software uses a unique report template file, which only supports A4 in
portrait. If printing in letter size portrait is required, take the following steps to
replace the template file with that for letter size that is included in the installation
disk of this software.
1. Turn on the power to a PC. After Windows has started, insert the installation
disk of this software into the CD-ROM drive.
2 Page Setup This example assumes that drive "E" is assigned to the CD-ROM drive.
2. Copy the following folder.
E:\English\Report Template\Letter_size
3. Open the installation folder of this software ( folder set in step 7 of "1.2
Installing Software".)
If the default setting has not been changed, the path to the folder is:
C:\Program Files\shimadzu\UVPerfVal
4. The "Reports" folder is under the installation folder. Change the folder name
to "A4reports".
5. Paste the folder that was copied in step 2 to the installation folder, and change
the folder name to "Reports".

UV Performance Validation Software 63

6 Menu Commands

6.2 [View] Menu

Table 6-3

Command Description Tool Button Reference

Displays the window showing the results of test

Results
items already completed.

Section 2.3

Displays the window showing the test status

View Test
currently in progress.

Changes the line color, line width, and

Graph Properties background color of the graph showing test — Section 6.2.1
results.

6.2.1 Setting Graph Drawing

Fig.6-3

Table 6-4

No. Display Description

Displays data currently shown on the graph tab.
1 Data
Click (highlight) data such as line color you want to change.
Select the type of graph line.
2 Line Type
Click (highlight) the desired line type and click [Apply] to apply your selection.
Select the mark for data points in a graph.
3 Mark
Click (highlight) the desired mark and click [Apply] to apply your selection.
Select the color of graph line.
4 Color When the Color Palette window appears, click the desired color and click [OK]
to apply your selection.
5 Apply Confirm the applied or changed setting.

64 UV Performance Validation Software

 6.3 [Test] Menu

6.3 [Test] Menu

Table 6-5

Command Description Tool Button Reference

Displays the [Edit Test Conditions] window.

Edit Conditions You can select items for testing, change order, and Section 4.2
edit detailed condition for each test item.

Displays the list of test conditions currently

configured.
You can check the information of acceptance
criteria or test tools, or print data.
View Conditions Section 2.7.2
If you log in with an account that does not have
rights to change test conditions, the view
conditions list tool button appears instead of the
set conditions button.
Displays the list of test tools required for test
View Tools — Section 4.3.1
items shown in the Batch of Test list.
6
Starts tests for items with a checked box in the
Start Section 4.3.3
Batch of Test list.

Stops all test processes, or only aborts (skips) the

Stop Section 4.3.3
process of the test item currently in progress.

UV Performance Validation Software 65

6 Menu Commands

6.4 [Instrument] Menu

Table 6-6

Command Description Tool Button Reference

Configures the communication port connected to
the spectrophotometer, and changes the instrument
name.
Configure — Section 1.4
In the Configure window, you can also check the
instrument type that can be connected and for
which the test condition file can be loaded.

Starts (connects) communication with the

Connect Section 1.5
spectrophotometer.

Ends (disconnects) communication with the

Disconnect Section 1.7
spectrophotometer.

This command has the spectrophotometer

recognize the current status of multi-cell used for
testing, and initializes the spectrophotometer if
Init MultiCell — (None)
necessary.
This function is automatically activated when the
test is started.

6.5 [Security] Menu

Table 6-7

Command Description Tool Button Reference

Changes account passwords, and changes
available functions. Section 1.6
Administrative —
This menu is only enabled when you log in as a Section 2.7.1
"system administrator".

66 UV Performance Validation Software

 6.6 [Help] Menu

6.6 [Help] Menu

Table 6-8

Command Description Tool Button Reference

Help Displays the Help window of this software. Section 6.6.1

Displays the version number of this software and

the information of the instrument currently
connected.

About — (None)
6

Fig.6-4

UV Performance Validation Software 67

6 Menu Commands

6.6.1 Help

This page is intentionally left blank.

Fig.6-5

Table 6-9

No. Display Description

1 [Contents] tab Searches the contents for the desired text.
2 [Keyword] tab Searches the keyword list for the desired text.
3 [Search] tab Searches the help file for the desired text.

68 UV Performance Validation Software

7 7 Troubleshooting

If a problem occurs, use the information in this section to confirm the problem before requesting a
repair.
Contact your Shimadzu representative if the error cannot be resolved through the remedial action
7
described below, or if the problem is not described here.
7
7.1 Test Result Fails 7
If any semi-automatic test item using multi-cell has failed the acceptance criteria, the multi-cell may have
an abnormality.
7
Retest the failed item in a "not using multi-cell" condition.
7
See "2.4 Test Using Multi-Cell".
7
7.1.1 "Failed" Appears in Initialization Setting Record
Check the symptom according to the instructions in the Instruction Manual of the spectrophotometer.
7
7.1.2 Wavelength Accuracy Failed
Table 7-1
7
No. Typical Cause Remedial Action
May be affected by changed environmental temperature or installation
7
conditions. Connect to the UVProbe Software, select [Configure] from

1
Installation environment has
changed after start of test.
the [Instrument] menu, and execute [Wavelength Recalibration] in the
Maintenance tab. 7
(Only UV-2600/2700 series)
For more information, refer to the "UV-2600/2700 Instruction
Manual".
7
Any test tool (including
2 lamps) or test condition has a
failure.
Follow the following instructions to make sure that the lamps or test
tools have no abnormalities, and test conditions have no problems. 7
(When a test tool is used)
An entered verified
7
Recheck the calibration certificate attached to the test tool, and also
3 wavelength or verified value
recheck configured conditions.
was incorrect.
The band-pass (slit width)
7
specified as a test condition in The slit width for the test is specified in the "Measurement Parameters"

4
the calibration certificate of
the test tool does not match
tab.
Use the test tool calibrated with the same band pass as the slit width
7
the slit width specified for the specified in the measurement parameters tab.
test. 7
UV Performance Validation Software 69
7 Troubleshooting

No. Typical Cause Remedial Action

This software cannot use such a wavelength.
Remove the target wavelength in the Test Tool Setting window, or
The absorption peak of
uncheck the test wavelength box in the Detailed Condition Setting
wavelength is broad, and
5 window.
thereby a noise is detected as
a peak wavelength.
For the operation method, see "5.1 Details of Test Items" or the help of
the software.
A portion where the beam of
Remove contamination according to the instructions given by the
6 the test tool passes has a flaw
supplier of the test tool, or recalibrate the test tool.
or contamination.
(When a deuterium lamp is used)
Check that communication is disconnected, and turn off the power of
the spectrophotometer. Then turn on the power again. If the default
setting of the "D2 Lamp Energy Check" fails, the deuterium lamp may
not light up.
7 The lamp is turned off. Check the light-up status of the lamp according to instructions
(troubleshooting) in the Instruction Manual of the spectrophotometer.

Do not look straight into the lamp while it is lit up. Doing so may
damage your eyes.
(When a low-pressure mercury lamp is used)
The power of the low- Recheck the installation status according to the instructions in the
8 pressure lamp unit is turned Instruction Manual of the low-pressure mercury lamp unit, and turn on
off. the power switch.
Check the light-up status of the lamp according to the instructions in
the Instruction Manual of the low-pressure mercury lamp unit.
9 The lamp is turned off.
Do not look straight into the lamp while it is lit up. Doing so may
damage your eyes.

7.1.3 Wavelength Repeatability Failed

Table 7-2

No. Typical Cause Remedial Action

Test the "WL Accuracy" item using the same tool or lamp.
No absorption peak (emission
1 line peak) is in the measuring If a peak also cannot be detected in the test for "WL Accuracy", see
range. "7.1.2 Wavelength Accuracy Failed" to check the symptom and take
proper action.
This software cannot use such a wavelength.
Remove the target wavelength in the Test Tool Setting window, or
The absorption peak of the
uncheck the test wavelength box in the Detailed Condition Setting
wavelength is broad, and
2 window.
thereby a noise is detected as
a peak wavelength.
For the operation method, see "5.1 Details of Test Items" or the help of
the software.

70 UV Performance Validation Software

 7.1 Test Result Fails

7.1.4 Photometric Accuracy Failed

Table 7-3

No. Typical Cause Remedial Action

A portion where the beam of
Remove contamination according to the instructions given by the
1 the test tool passes has a flaw
supplier of the test tool, or recalibrate the test tool.
or contamination.
An entered verified
Recheck the calibration certificate attached to the test tool, and also
2 wavelength or verified value
recheck configured conditions.
was incorrect.
The band-pass (slit width)
specified as a test condition in The slit width for the test is specified in the "Measurement Parameters"
the calibration certificate of tab.
3
the test tool does not match Use the test tool calibrated with the same band pass as the slit width
the slit width specified for the specified in the measurement parameters tab.
test.
Some test tools have a reference value that fluctuates a great deal
The sample temperature depending on the temperature.
specified as a test condition in It is recommended that either the sample be retested in an environment
the calibration certificate of where the temperature of the test tool falls within about ±3 °C of the
4
the test tool very differs from sample temperature specified as a test condition, or the temperature of
the environmental the test tool itself be controlled. The 6-Cell Electronic Temperature
temperature during the test. Control Cell Holder (CPS-240A), can be used to control the filter
temperature during a test. 7
Check "WL Accuracy".
Wavelength is out of
5
alignment. If "WL Accuracy" fails the criteria, see "7.1.2 Wavelength Accuracy
Failed" to check the symptom and take proper action.

7.1.5 Photometric Repeatability Failed

Table 7-4

No. Typical Cause Remedial Action

If the life span (hours) of the light source lamp has been reached, refer
1 There is a heavy noise. to the Instruction Manual of the spectrophotometer and replace the
lamp.
Perform the test again without inserting or removing the test tool.
If the test succeeds in the same test tool condition, the cell holder may
A test tool is not held have a failure, or the test tool may not have been correctly inserted or
2
correctly (cell holder fails). removed.
If the test failed again even after you followed the messages to perform
the test, contact your Shimadzu representative.

UV Performance Validation Software 71

7 Troubleshooting

7.1.6 Resolution Failed

Table 7-5

No. Typical Cause Remedial Action

(Absorbance Ratio)
A portion where the beam of
Remove contamination, or insert toluene/hexane solution into another
1 the test tool passes has a flaw
cell and perform the test again.
or contamination.
If the waveform obtained during the test largely differs from that
shown in the following figure (example of measuring toluene/hexane
solution at slit 1 nm), the used test tool (toluene/hexane solution) may
be deteriorated, or its concentration may have changed. Change the test
tool and perform the test again.

Toluene/hexane solution is
2 deteriorated. (Concentration
has changed.)

Fig.7-1

(Bandwidth)
Test the "WL Accuracy" item using the same tool or lamp.

No emission line is in the

3 If a peak also cannot be detected in the test for "WL Accuracy", see
measuring range.
"7.1.2 Wavelength Accuracy Failed" to check the symptom and take
proper action.
A micro amount of hydrogen atoms are in the deuterium lamp used for
UV-2400PC/2500PC series the test. The emission line spectrum of hydrogen atoms occurs at
Affected by hydrogen atoms 656.28 nm, which may affect the result of resolution test conducted
4 using UV-2400/2500 series.
contained in a deuterium
lamp.
See "8.3.2 Influence by Hydrogen Emission Line".

7.1.7 Stray Light Failed

Table 7-6

No. Typical Cause Remedial Action

A portion where the beam of
Remove contamination, or insert test solution into another cell to
1 the test tool passes has a flaw
perform the test again.
or contamination.
The used test tool (solution) may be deteriorated or its concentration
A test tool has deteriorated.
2 may have changed.
(Concentration has changed.)
Change the test tool and perform the test again.

72 UV Performance Validation Software

 7.1 Test Result Fails

7.1.8 Noise Level Failed

Table 7-7

No. Typical Cause Remedial Action

The life span (hours) of a
Replace the light source lamp according to the instructions in the
1 light source lamp has been
Instruction Manual of the spectrophotometer.
exceeded.

7.1.9 Baseline Flatness Failed

Table 7-8

No. Typical Cause Remedial Action

The life span (hours) of a
Replace the light source lamp according to the instructions in the
1 light source lamp has been
Instruction Manual of the spectrophotometer.
exceeded.

7.1.10 Photometric Drift (Baseline Stability) Failed

Table 7-9

No. Typical Cause Remedial Action

The life span (hours) of a
Replace the light source lamp according to the instructions in the
1 light source lamp has been
exceeded.
Instruction Manual of the spectrophotometer.
7
Control the room temperature using an air conditioner or other
equipment to reduce fluctuation of the environmental temperature
The environmental
2 (within the range of about 2 °C/h) during the test. When an air
temperature fluctuates.
conditioner is used, be sure that the spectrophotometer will not be
exposed to airflow from the equipment.

UV Performance Validation Software 73

7 Troubleshooting

7.2 Error Messages

If an error persists even after you took the remedial action described in the following for each error,
contact your Shimadzu representative.

7.2.1 Error Message List

Table 7-10

No. Message Cause and Remedial Action

When software starts
The file storing test conditions has corrupted what was configured
when you exited the software the last time.
If you click [OK] to close the message, the software will start with the
The test conditions file could
1 initial test conditions.
not be opened.

For information about initial test conditions, see "8.1 Recommended

Value List".
The file storing security information does not exist or the file is
corrupted. If you click [OK] to close the message, the software will
start with the initial settings. This also initializes (erases) the password
Failed to read security for logging in.
2
information.

For information about the default settings for each account, see "2.7.1
Restricting Functions Based on Account".
When loading or saving test condition/results files
The test condition file may not be in the folder where it was registered,
The test conditions file could
3 or the file may be corrupted. Check whether the file is in the folder
not be opened.
shown in the window.
The test results file could not Contents of the file may be corrupted.
4
be opened. Contact your Shimadzu representative.
The test conditions file could
5 Free space in the hard disk or USB memory specified as the destination
not be saved.
is insufficient.
The test results file could not Check the amount of available free space in the destination.
6
be saved.
The selected file is not of a
When loading a file by drag & drop
7 format recognized by UV
Files created by other applications cannot be loaded.
Performance Validation.
Can not read a file when When loading a file by drag & drop
8
measuring data. A test results file cannot be opened during a test.

74 UV Performance Validation Software

 7.2 Error Messages

No. Message Cause and Remedial Action

This software can only load test condition files created by the
instrument that was configured during installation.

The instrument currently

See "6.4 [Instrument] Menu".
selected is not the same as the
9 Load the test condition file in the same environment as it was created
instrument used to create the
in, and check or print the content of the test conditions. Then create
file.
new test conditions in the environment of the other instruments.

See "2.7.2 Convenient Functions for Test Conditions".

When establishing communication with the instrument
This software only controls the instrument type configured during
installation.

The instrument currently See "6.4 [Instrument] Menu".

selected with software is If you want to change the instrument type to be controlled, reinstall this
10
different from connected software.
instrument. Please note, however, that the test condition file currently used cannot
be used if you change the instrument type.

See "1.2 Installing Software".

Currently establishing or testing communication with the instrument
Content of the error differs depending on the indicated code number.
7
Communication error
11
(Code number) has occurred.
See "7.2.2 Error Code List".
Check whether the 6-cell (or CSP-240) has been installed and wired
12 Failed to initialize Multicell.
correctly according to the instructions in its Instruction Manual.
You have never configured initialization settings of the
Failed to get results of spectrophotometer with this software, or the record obtained the last
initialization. Restart the time may be lost. Restart the instrument, and configure the
13 initialization settings.
instrument. After the restart,
connect the software.
See "1.5 Establishing Communication with Instrument".
The power of the instrument currently connected may be turned off or
the communication cable may be unplugged. Check that the power of
14 Communication Time Out ! the instrument is turned on and that the communication cable is
correctly connected to the PC and instrument, and then restart
communication.
Others
Restart the software.
Failed to read security
15 If a similar message appears also when the software starts, the setup
information.
file is corrupted. Configure the security settings again.
The security setting (privileges) may have been changed (to read-only)
Failed to write security to access the folder where the setup file exists.
16
information. If you have changed the setting, restore the original setting (allowing
read and write access).

UV Performance Validation Software 75

7 Troubleshooting

No. Message Cause and Remedial Action

The file required for printing does not exist in the specified folder (the
file was moved or deleted from the folder), or the content of the file is
Cannot open Report Format corrupted.
17 Reinstall this software.
File.

See "1.2 Installing Software".

7.2.2 Error Code List

The following lists code numbers displayed when communication is established. ( No.11 in
"7.2.1 Error Message List")
Table 7-11

Code Description Cause and Remedial Action

When communication is established
Check the following and establish communication again:
• The instrument is turned on.
• The communication cable is correctly connected.
• The instrument is not connecting with other software.
• The COM port settings are correctly configured.
A timeout error has occurred
1
during communication. See "1.4 Configuring Communication Port".
When connected
The power of the instrument currently connected may be turned
off or the communication cable may be unplugged. Check that
the power of the instrument is turned on and that the
communication cable is correctly connected to the PC and
instrument, and then restart communication.
An abnormal operation of the spectrophotometer has occurred.
3 A hardware error has occurred. Turn off the power of the instrument, and contact your Shimadzu
representative.
The test condition file currently used has a problem.
Check details of the test conditions to see whether an abnormal
6 An invalid parameter. value was set.

See "2.7.2 Convenient Functions for Test Conditions".

The instrument may not be recognized immediately after its
The instrument has not been
8 power is turned on. After turning on the power, wait about
found.
10 seconds before clicking [Connect] on the tool button bar.
The instrument being connected is not supported by this
software. Check the instrument (type) currently being connected.
100 The instrument type is invalid.
For instrument types to which this software can connect, see
"2.1 Intended Instrument Types".

76 UV Performance Validation Software

 7.3 Others

7.3 Others
Table 7-12

No. Description Cause and Remedial Action

Log in as a system administrator and change the password.
If you also forgot the system administrator password, contact your
1 Forgot your login password. Shimadzu representative.

See "1.6 Setting Password".

This software could not recognize the spectrophotometer.
• The communication port settings of the instrument may be
Even after the "Waiting to incorrect. Check the settings.
establish the communication."
2 message dialog disappears, See "1.4 Configuring Communication Port".
communication with the • Power to the instrument may not be turned on. Check the power.
instrument is not established. • The communication cable may not be correctly connected. Check
the cable.
After carrying out the check, click [Connect] again.

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7 Troubleshooting

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78 UV Performance Validation Software

8 8 Appendixes

8.1 Recommended Value List 8

At least the recommended test items (No. columns are highlighted in table 8-1) and recommended
values for acceptance criteria are checked during the periodic inspection by Shimadzu or relevant
service sectors to determine abnormalities of instruments.
8
The recommended test items and values are used for the test items and conditions (initial conditions)
that are set when you start this software immediately after installation or when you select [New] from 8
the [File] menu ( "6.1 [File] Menu").

8
Photometric accuracy and photometric repeatability can be tested in transmittance but no acceptance
criteria of Shimadzu's recommendation are available (as Shimadzu recommends tests in absorbance). As
the software specifications do not allow a blank value, the initial (recommended) value in transmittance is
"1.00 %T".
8
For the method used to set acceptance criteria when tested in transmittance, see "5.1 Details of Test
8
Items" or the help of the software.
Table 8-1
8
UV-1600 UV-2400 UV-2500 UV-2600 UV-2700
No. Test Item

Initialization setting
Series Series Series Series Series
8
1 All items must be indicated with "Passed".
log
WL Accuracy (nm max.) 8
Using reference
2 ±0.50
filter
Using deuterium
8
3 ±0.30
lamp

4
Using low-pressure
mercury lamp
(N/A) ±0.30 8
8
WL Repeatability (nm max.)
Using reference
5 ±0.20
filter

6
Using deuterium
lamp
±0.20 8
Using low-pressure
7
mercury lamp
(N/A) ±0.20
8
Photometric Accuracy (Abs max.)

8
In the vicinity of
1 Abs (10 %T)
±0.004 ±0.004 8
In the vicinity of
9
0.5 Abs (30 %T)
±0.004 ±0.004
8
UV Performance Validation Software 79
8 Appendixes

UV-1600 UV-2400 UV-2500 UV-2600 UV-2700

No. Test Item
Series Series Series Series Series
Photometric Repeatability (Abs max.)
In the vicinity of
10 ±0.002 ±0.004
1 Abs (10 %T)
In the vicinity of
11 ±0.002
0.5 Abs (30 %T)
Resolution
Absorbance Ratio
12 1.0 1.5 (Slit: 1.0 nm)
(min.)
Less than
13 Bandwidth Less than 0.21 nm Less than 0.15 nm
2.1 nm
Stray Light (% max.) *
14 198 nm (N/A) 1.0 1.0
15 220 nm 0.05 0.015 0.002 0.015 0.002
16 340 nm 0.05 0.015 0.002 0.015 0.002
17 370 nm (N/A) 0.015 0.002
Others
Wavelength:
Wavelength: 500 nm Wavelength: 500 nm
Noise Level 700 nm
18 P-P: 0.001 P-P: 0.001
(Abs max.) P-P: 0.002
RMS: 0.001 RMS: 0.0001
RMS: 0.002
Wavelength
Wavelength range: Wavelength range:
Baseline Flatness range: 190 nm
19 195 nm to 850 nm 200 nm to 860 nm
(Abs max.) to 1100 nm
±0.001 ±0.001
±0.002
Photometric Drift Wavelength: Wavelength: 500 nm Wavelength: 700 nm
20 (Baseline Stability) 700 nm 0.001 0.001
(Abs/H max.) 0.002

* A negative stray light value may be obtained due to noise. The test result will be treated as a pass even if a
negative stray light value is obtained as long as the value is small enough to be judged to have been caused
by noise.

80 UV Performance Validation Software

 8.2 Test Method Using UVProbe

8.2 Test Method Using UVProbe

This section describes the method used to measure and test special items this software cannot test by
using the UVProbe Software, which can control spectrophotometers and process their data.

8.2.1 Testing Resolution Using Benzene Vapor

WARNING
When handling benzene, take appropriate safety measures by wearing adequate working
clothes, protective goggles, masks, and gloves in accordance with the Material Safety Data
Sheet (MSDS) guidelines created by the relevant reagent manufacturers.
Failure to observe this may result in an accident.
If an accident involving chemicals occurs, take relevant first-aid and emergency measures according
to the instructions provided by the relevant organizations.

1 Encapsulate approximately 2 μl (a half drop) of benzene in the quartz cell with an

airtight stopper (optical path length: 10 mm).

2 Set the measurement mode of the spectrophotometer to absorbance, the scanning

speed to low, and the slit width to 0.1 nm.

3 Set the wavelength scanning range to between 252 nm and 248 nm, and after
baseline correction, set the above quartz cell in the sample compartment and measure 8
spectrum.

4 Adjust concentration of the benzene vapor so that the absorption peak in the range
251 nm to 249 nm (encircled in the following figure) will be 0.5 Abs ±0.2 Abs.

Fig.8-1

To attenuate the concentration, open the cell plug and leave it for a while. To increase the
concentration, warm up the cell with body heat, or add approximately 1 μl of benzene.

UV Performance Validation Software 81

8 Appendixes

5 Perform wavelength scanning in the range 252 nm to 248 nm to log the spectrum.

6 Check that the obtained spectrum (encircled in the figure shown in step 4) has three
separated peaks with an interval of approximately 0.2 nm.

8.2.2 Testing Stray Light Using Mesh Filter

The following describes the procedure for testing the stray lights (of 220 nm and 340 nm) using mesh
filter P/N 206-82299-91 for UV-2500PC series.

„ Obtaining a stray light value at 220 nm

1 Start the UVProbe Software and enter the spectrum measurement mode (spectrum
module).

2 Set measuring parameters as follows.

Measuring Mode: Transmittance
Wavelength Range: 230nm - 210nm
Scan Speed: Fast
Slit Width: 2.0nm

3 Make sure that nothing is inserted into cell holders and start baseline correction.

4 After baseline correction is completed, set [Go To WL] to 220 nm, insert a mesh filter
into the cell holder on the sample side, and close the sample compartment cover.

5 Write down the transmittance displayed in the lower right of the window.
Let this value be "transmittance A".
In sample compartment > Sample (S) side: Mesh filter, Reference (R) side: Air

6 Move the mesh filter from the sample side cell holder to the reference side, and in its
place, insert a shutter block into the sample side cell holder.
In sample compartment > S side: Shutter block, R side: Mesh filter

7 Press [Start] to start measurement, and after the measurement is completed, click
[Point Pick] from the [Operations] menu, and write down the transmittance at 220 nm.
Let this value be "transmittance B".
* Click [Settings] from the [View] menu to set the number of digits to 3 after the decimal point to be
displayed for Y-axis value (photometric value).

8 Remove the shutter block from the sample side cell holder, and in its place, insert a
quartz cell containing 10 g/l of sodium iodide (NaI) solution into the cell holder.
In sample compartment > S side: Cell with NaI solution, R side: Mesh filter

9 Press [Start] to start measurement, and after the measurement is completed, click
[Point Pick] from the [Operations] menu, and wite down the transmittance at 220 nm.
Let this value be "transmittance C".

82 UV Performance Validation Software

 8.2 Test Method Using UVProbe

10 Apply recorded transmittance values A to C to the following expression and obtain the
stray light value at 220 nm.
Stray light value = [(Transmittance C − Transmittance B) × Transmittance A] ÷ 100

„ Obtaining a stray light value at 340 nm

1 Set measuring parameters as follows.

Measuring Mode: Transmittance
Wavelength Range: 350nm - 330nm
Scan Speed: Fast
Slit Width: 2.0nm

2 Make sure that nothing is inserted into cell holders and start baseline correction.

3 After baseline correction is completed, set [Go To WL] to 340 nm, insert a mesh filter
into the cell holder on the sample side, and close the sample compartment cover.

4 Write down the transmittance displayed in the lower right of the window.
Let this value be "transmittance A".
In sample compartment > Sample (S) side: Mesh filter, Reference (R) side: Air

5 Move the mesh filter from the sample side cell holder to the reference side, and in its
place, insert a shutter block into the sample side cell holder.
In sample compartment > S side: Shutter block, R side: Mesh filter

8
6 Press [Start] to start measurement, and after the measurement is completed, click
[Point Pick] from the [Operations] menu, and write down the transmittance at 340 nm.
Let this value be "transmittance B".
* Click [Settings] from the [View] menu to set the number of digits to 3 after the decimal point to be
displayed for Y-axis value (photometric value).

7 Remove the shutter block from the sample side cell holder, and in its place, insert a
quartz cell containing 10 g/l of sodium nitrite (NaNO2) solution into the cell holder.
In sample compartment > S side: Cell with NaNO2 solution, R side: Mesh filter

8 Press [Start] to start measurement, and after the measurement is completed, click
[Point Pick] from the [Operations] menu, and write down the transmittance at 340 nm.
Let this value be "transmittance C".

9 Apply recorded transmittance values A to C to the following expression and obtain the
stray light value at 340 nm.
Stray light value = [(Transmittance C − Transmittance B) × Transmittance A] ÷ 100

As the stray light value at 340 nm must be measured while the WI lamp is used, set the light source switch
wavelength to 330 nm before starting measurement.

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8 Appendixes

8.3 Reference Data

8.3.1 What is Stray Light?
Stray light is the total of the light of the wavelength deviated from a certain spectrum width that has
the set wavelength of the spectrometer placed in the center and the light emitted from spectrometer
that passes the side of the sample instead of transmitting it.
For example, if 0.1 % of stray light is included in the monochromatic light when a sample with the
wavelength of λo and absorbance of 2 (transmittance 1 %) is measured, the transmitted light will
have 1.1 % of transmittance (absorbance 1.959), causing 2 % error. It is because 0.1 % stray light is
added in addition to 1 % of the light with the wavelength of λo.
As known from this, the higher the sample absorbance is, the larger the error due to stray light
becomes.
To reduce stray light, it is generally effective to form a double-monochromator by connecting two
monochromators. Although it is expensive due to the complex mechanism, the amount of stray light
is reduced from one-fiftieth (1/50) to one-one thousandth (1/1000) of that of normal single
monochromater.
Table 8-2 Absorbance Error due to Stray Light

Stray Light Absorbance

(%) 0.8 1.0 1.2 1.5 1.8 2.0 2.5 3.0 4.0
1 0.0222 0.0370 0.0595 0.1150 0.2081 0.2967 0.6150 1.0370 2.0000
0.5 0.0133 0.0190 0.0309 0.0615 0.1169 0.1739 0.4096 0.7759 1.7059
0.2 0.0045 0.0077 0.0126 0.0257 0.0507 0.0783 0.2119 0.4762 1.3213
0.1 0.0022 0.0038 0.0063 0.0130 0.0261 0.0409 0.1188 0.3005 1.0409
0.05 0.0011 0.0019 0.0032 0.0065 0.0132 0.0209 0.0635 0.1758 0.7799
0.02 0.0004 0.0007 0.0012 0.0026 0.0053 0.0085 0.0365 0.0790 0.4770
0.01 0.0002 0.0003 0.0006 0.0013 0.0026 0.0042 0.0134 0.0413 0.3009
0.005 0.0001 0.0001 0.0003 0.0006 0.0013 0.0021 0.0067 0.0211 0.1791
0.002 0.0000 0.0000 0.0001 0.0003 0.0005 0.0008 0.0027 0.0085 0.0791
0.001 0.0000 0.0000 0.0000 0.0001 0.0002 0.0004 0.0013 0.0043 0.0413
0.0001 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0001 0.0004 0.0043

(Test and Engineering Vol.9 No.9, 702, 1981)

84 UV Performance Validation Software

 8.3 Reference Data

8.3.2 Influence by Hydrogen Emission Line

A micro amount of hydrogen atoms are in the deuterium lamp used for the resolution and other tests.
This emission line spectrum of hydrogen atoms occurs at 656.28 nm, which is in proximity to
656.10 nm of the emission line of the deuterium lamp. This may affect test results of resolution
obtained using UV-2400/2500 series when testing resolution (spectral band width) with the 0.2 nm
slit width.
Figure A below shows the emission line spectra at 656.1 nm, measured by setting the slit width to
0.1 nm and data interval to 0.01 nm using Shimadzu spectrophotometer UV-3600. "H" is a spectrum
generated by hydrogen, and "D" by deuterium.
Figure B shows the emission line spectra when this is measured by setting the slit width to 0.2 nm. As
the hydrogen's spectrum overlaps, two spectra appear to be a single emission line spectrum. The
resolution (half width) at this time is 0.24 nm.
The default (recommended) value as an acceptance criterion is less than 0.21 nm for UV-2400/2500
series. As hydrogen atoms have a significant affect in this example, the result fails the criteria.
However, this is not because the spectrophotometer has a resolution-related failure.
Energy

Energy

A: Slit width 0.1 nm B: Slit width 0.2 nm

Fig.8-2

When the spectrum is measured using UV-2400PC/2500PC series, the applicable minimum sampling
interval is 0.05 nm, and thereby a 0.1 nm resolution cannot be checked with the spectral band width of the
emission line.
To check a 0.1 nm resolution using UV-2400PC/2500PC series, you can only measure benzene vapor and
obtain its shape to determine the resolution.

See "8.2.1 Testing Resolution Using Benzene Vapor".

UV Performance Validation Software 85

8 Appendixes

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86 UV Performance Validation Software

 Index

A M
Account ......................................................7, 10, 22 Menu bar ....................................................... 13, 61
Automatic .............................................................31 Multi cell ........................................................ 18, 28
Automatic standby for test....................................25

N
B Noise level........................................................... 31
Baseline flatness ..................................................31
Batch of test pane ..........................................13, 15
P
Password ........................................................ 7, 10
C Photometric accuracy.................................... 28, 56
COM Port number ..................................................8 Photometric drift (baseline stability) .................... 31
Condition file ........................................................19 Photometric repeatability............................... 28, 57
CPS................................................................18, 28 Print layout .................................................... 20, 63

D R
Decision digit count ..............................................33 Resolution (absorbance ratio) ....................... 29, 58
Detailed condition setting .....................................53 Resolution (bandwidth) ....................................... 31
Result file ...................................................... 19, 49
Result pane ......................................................... 13
E
Expiration date ...............................................44, 63
S
Security control ............................................. 10, 22
F
Semi-automatic ................................................... 28
File information pane......................................13, 14 Serial number ............................................ 4, 11, 12
File properties ......................................................50 Stray light ................................................ 29, 59, 84
System setting..................................................... 63
H System status pane....................................... 13, 16

Help................................................................53, 68
T
I Test condition ...................................................... 36
Test item ................................................. 31, 33, 53
Initialization setting.................................................9
Test tool ........................................................ 28, 44
Instrument name ..............................................4, 12
Tool button bar .............................................. 13, 61
Instrument type ................................................4, 12

V
L
View result window........................................ 17, 48
Log in .....................................................................7
View test window........................................... 17, 48

UV Performance Validation Software 87

Index

W
Wavelength accuracy.................................... 29, 54
Wavelength accuracy (deuterium lamp).............. 31
Wavelength accuracy
(low-pressure mercury lamp) .............................. 31
Wavelength repeatability............................... 29, 55
Wavelength repeatability (deuterium lamp)......... 31
Wavelength repeatability
(low-pressure mercury lamp) .............................. 32

88 UV Performance Validation Software

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