Certificate of Analysis
Reference Substance
JWH-203 (2-(2-Chlorophenyl)
Chlorophenyl)-
1-(1-pentylindol-3-yl)ethanone)
yl)ethanone)
Catalogue Number: LGCFOR
LGCFOR1275.70 Long-term
term Storage: 2 to 8 °C, dark
Lot Number: 38407
Appearance: light yellow solid
Molecular Formula: C21H22ClNO
Melting Point: 103 °C
Molecular Weight: 339.86
CAS Number: [ 864445-54-5
864445 ] Assay ‘as is’: 98.6 %
O Cl
Date of shipment: 2016
2016-May-20
This certificate is valid for two years from the date of shipment
provided the substance is stored under the recommended
conditions.
Release Date: 2013-10
10-10 LGC GmbH
Dr. Sabine Schröder
Product Release
LGC GmbH, Im Biotechnologiepark, TGZ
GZ II, D-14943 Luckenwalde, Germany 1
6 pages
© 2012 LGC limited. All rights reserved. LGC Standards is part of the LGC Group.
LoGiCal is a registered trademark of LGC Standards GmbH
I. Identity
The identity of the reference substance was established by following analyses.
1
Ia. H-NMR Spectrum
Conditions: 400 MHz, DMSO-d6
The structure is confirmed with the signals of the spectrum and their interpretation.
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Ib. Mass Spectrum
Method: 4.5 kV ESI; vaporization temperature: 200 °C, direct inlet
m/z fragments
340 [ MH ]
214 [ M – C7H6Cl ]
188 [ M – C5H11NCH – H2O – Cl ]
125 [ C7H6Cl ]
The signals of the mass spectrum and their interpretation are consistent with the structural formula.
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Ic. IR Spectrum
Method: Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) Spectroscopy
The signals of the IR spectrum and their interpretation are consistent with the structural formula.
II. Purity
The purity of the reference substance was analysed by high performance liquid chromatography
(HPLC).
HPLC Conditions:
Column: Conditions: Detector: Injector:
Hypersil Gold (C18) 1.0 ml/min, 40 °C DAD Auto
5 µm, 150 x 4.6 mm 0 – 7 min Water/Acetonitrile 35/65 215 nm 3 µl; 0.0724 mg/ml in
7 – 10 min Water/Acetonitrile to 10/90 Acetonitrile
10 – 13 min Water/Acetonitrile to 35/65
13 – 18 min Water/Acetonitrile 35/65 (v/v);
0.1 % H3PO4
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© 2012 LGC limited. All rights reserved. LGC Standards is part of the LGC Group. LoGiCal is a registered trademark of LGC Standards GmbH
500 Surveyor PDA 4-215nm 500
Retention Time
400 400
300 300
mAU
mAU
200 200
100 100
12.21
2.09
5.95
7.53
0 0
0 2 4 6 8 10 12 14 16 18
Minutes
Area Percent Report - Sorted by Signal
Pk # Retention Time Area Area %
1 2.09 2073 0.04
2 5.95 1560 0.03
3 7.53 4967852 99.38
4 12.21 27467 0.55
Totals 4998952 100.00
For the calculation the system peaks were ignored. The content of the analyte was determined as ratio
of the peak area of the analyte and the cumulative areas of the purities, added up to 100 %.
Results:
Average 99.38 %
Number of results n=3
Standard deviation < 0.01 %
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© 2012 LGC limited. All rights reserved. LGC Standards is part of the LGC Group. LoGiCal is a registered trademark of LGC Standards GmbH
III. Water Content
Method: Karl Fischer titration
No significant amounts of water were detected (< 0.05 %).
IV. Residual Solvents
1
Method: H-NMR
Result: 0.71 % Ethyl acetate
0.07 % Cyclohexane
V. Final Result
Total impurities (HPLC) 0.62 %
Water content n. d. (not detected)
Residual solvents 0.78 %
1
Assay (100 % method) 98.60 %
The assay is assessed to be 98.6 % ‘as is’
The assay 'as is' is equivalent to the assay based on the not anhydrous and not dried substance
respectively.
1
The calculation of the 100 % method follows the formula:
Purity HPLC (%)
Assay (%) = (100 % - KF - RES) * 100 %
Water (KF) and Residual solvents (RES) are considered as absolute contributions, HPLC purity is considered as relative
contribution.
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© 2012 LGC limited. All rights reserved. LGC Standards is part of the LGC Group. LoGiCal is a registered trademark of LGC Standards GmbH
