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This document outlines a procedure for estimating dissolved oxygen levels in a water sample using Winkler's method. It details the materials, reagents, and schematic diagram needed. Tables show the results of standardizing sodium thiosulfate and determining dissolved oxygen. Calculations are presented, and the conclusion indicates the water sample had a dissolved oxygen level of 2.88 mg/L, making the water not viable for drinking.

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Davinci Legaspi
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55 views3 pages

Huhuh

This document outlines a procedure for estimating dissolved oxygen levels in a water sample using Winkler's method. It details the materials, reagents, and schematic diagram needed. Tables show the results of standardizing sodium thiosulfate and determining dissolved oxygen. Calculations are presented, and the conclusion indicates the water sample had a dissolved oxygen level of 2.88 mg/L, making the water not viable for drinking.

Uploaded by

Davinci Legaspi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Virtual Lab #6

Objective: To estimate the Dissolved Oxygen (DO) level in the given water sample by Winkler’s method

Setup:

Materials:

 BOD Bottles
 Pipette
 50mL Conical Flask
 50mL Burette
 Beaker
 Dropper
 Spatula
 Burette Stand
 Funnel

Reagents:

 Manganese Sulfate
 Alkali Iodide Azide solution
 Concentrated Sulfuric Acid
 Sodium Thiosulfate solution
 Potassium Iodide powder
 Hydrochloric Acid
 0.25N Potassium Dichromate solution
 Starch indicator
Schematic Diagram:

Start the Determination of


Standardize the Sodium Add 2 mL of Manganese
Oxygen by filling the BOD
Thiosulfate using Sulfate solution to the
bottle with the water
Potassium Chromate BOD bottle using pipette
sample

Mix the sample by


Add 2 mL of the Add 2 mL of Alkali-Iodide-
inverting the BOD bottle
concentrated Sulfuric Azide reagent to the BOD
allowing precipitate to
Acid using the pipette bottle using pipette
settle at the bottom

Continue the titration


Transfer 200 mL of the
Add 2 mL Starch when the until the solution
sample to the conical
color of the solution becomes colorless then
flask then titrate it
changes to pale yellow record the observation
against Sodium
turning it to blue and compute for the DO
Thiosulfate
content

Results:

Table 1. Standardization of Sodium Thiosulfate

Normality of Volume of Burette Reading (mL) Volume of Normality of


K2Cr2O7 K2Cr2O7 Na2S2O3 (mL) Na2S2O3 (N)
solution (N) solution (mL) (Final – Initial)
Initial Value Final Value

0.025 10 0 10.1 10.1 0.025 (0.0247)

Table 2. Determination of Dissolved Oxygen in Water Sample

Volume of Normality of Burette Reading (mL) Volume of Dissolved


Sample (mL) Na2S2O3 (N) Na2S2O3 (mL) Oxygen (mg/L)
(Final – Initial)
Initial Value Final Value

201 10 0 3.6 3.6 2.88


Calculations:
For Standardization of Sodium Thiosulfate
Normality of K2Cr2O7 solution = 0.025 N
Volume of K2Cr2O7 solution = 10 mL
Volume of Na2S2O3 = 10.1 mL – 0 mL = 10.1 mL
0.025 N x 1 1 mL
N of Na2 S 2 O 3 = =0.2 5 N
10.1mL
For Dissolved Oxygen in Water Sample
Volume of Sample = 201 mL
Normality of Na2S2O3 = 10 N
Volume of Na2S2O3 = 3.6 mL – 0 mL = 3.6 mL
3.6 mL 1 mol 64 g 1
DO=10 N x x x x =2.88 mg/L
1000 mL 4 mol 1 mol 0.201 L
Conclusion:
The determination of Dissolved Oxygen in a water sample is necessary in order to assess
the quality of the water and the level of pollution on the source of the water. The Winkler’s
Modified Method is the most commonly used technique in order to measure the Dissolved
Oxygen concentration in freshwater structures. This is used as an indicator of the health and
how polluted the body of water is. The result of the experiment showed that the water sample
have a DO of 2.88 mg/L making the water not viable for drinking since the acceptable limit of
DO in water is 4 mg/L to 7 mg/L and anything less than 4 mg/L is considered to be extremely
harmful to drink.

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