7.75716.0004-xxxxxxxxxx msp.
September 2013
Notes on the measurement:
1.00598.0001
1.00598.0002 • Certain photometers may require a blank (distilled water without reagent).
• Wipe,
For photometric measurement the cells must be clean.
if necessary, with a clean dry cloth.
Spectroquant® • Measurement of turbid solutions yields false-high readings.
Chlorine Test Cl2 • The pH of the measurement solution must be within the range 4.5-5.5.
• end
The color of the measurement solution remains stable for 30 min after the
of the reaction time stated above.
for the determination of free chlorine • Inuctstheareevent of chlorine concentrations exceeding 25 mg/l, other reaction prod-
formed and false-low readings are yielded. In such cases it is advis-
USEPA approved for drinking water able to conduct a plausibility check of the measurement results by diluting
the sample (1:10, 1:100).
1. Method
In weakly acidic solution free chlorine reacts with dipropyl-p-phenylenediamine 8. Analytical quality assurance
(DPD) to form a red-violet dye that is determined photometrically. recommended before each measurement series
The method is analogous to EPA 330.5, APHA 4500-Cl2 G, and DIN EN ISO To check the photometric measurement system (test reagent, measurement
7393-2. device, handling) and the mode of working, a freshly prepared chlorine standard
solution containing 3.00 mg/l Cl2 (specifications see the website) can be used.
2. Measuring range and number of determinations Sample-dependent interferences (matrix effects) can be determined by means
of standard addition.
Cell Measuring range Number of Additional notes see under www.qa-test-kits.com.
mm mg/l Cl2 determinations
Characteristic quality data:
50 0.010 - 1.000 200 (Cat. No. 1.00598.0002) In the production control, the following data were determined in accordance with
20 0.02 - 3.00 or
1200 (Cat. No. 1.00598.0001)
ISO 8466-1 and DIN 38402 A51 (10-mm cell):
10 0.05 - 6.00
Standard deviation of the method + 0.049
For programming data for selected photometers / spectrophotometers see (mg/l Cl2)
www.service-test-kits.com. Coefficient of variation of the method + 1.5
(%)
3. Applications Confidence interval + 0.12
(mg/l Cl2)
Sample material: Number of lots 41
Swimming-pool water
Drinking water
Wastewater Characteristic data of the procedure:
Disinfectant solutions Measuring range
This test is not suited for seawater. mg/l Cl2
0.010 - 1.000 0.05 - 6.00
4. Influence of foreign substances Sensitivity: 0.007 0.03
This was checked in solutions containing 3.5 and 0 mg/l Cl2. The determination Absorbance 0,010 A
is not yet interfered with up to the concentrations of foreign substances given in corresponds to (mg/l Cl2)
the table. Accuracy of a measurement value max. ± 0.034 max. ± 0.17
(mg/l Cl2)
Concentrations of foreign substances in mg/l or %
Al3+ 250 Mn2+ 100 Br2 0.2
For quality and batch certificates for Spectroquant® test kits see the website.
Ca2+ 1000 NO2- 0.1 ClO2 0.2
CN- 0.1 S2- 0.1 I2 0.4 9. Notes
CO32- 1000 H2O2 0.05
Cr3+
Cr2O72-
250
0.1
O3
NaCl
0.05
10 %
• Reclose the reagent bottle immediately after use.
Cu2+ 100 NaNO3 10 % • Information on disposal can be obtained at www.disposal-test-kits.com.
Fe3+ 100 Na2SO4 10 %
5. Reagents and auxiliaries
The test reagent is stable up to the date stated on the pack when stored
closed at +15 to +25 °C.
Package contents:
Reagent Cl2-1: 1 bottle (Cat. No. 1.00598.0002) or
6 bottles (Cat. No. 1.00598.0001)
1 AutoSelector
Other reagents and accessories:
MColorpHastTM pH-indicator strips pH 5.0 - 10.0, Cat. No. 109533
MColorpHastTM pH-indicator strips pH 0 - 6.0, Cat. No. 109531
Sodium hydroxide solution 1 mol/l TitriPUR®, Cat. No. 109137
Sulfuric acid 0.5 mol/l TitriPUR®, Cat. No. 109072
Pipette for a pipetting volume of 10 ml
Rectangular cells 10, 20, and 50 mm (2 of each), Cat. Nos. 114946, 114947,
and 114944
6. Preparation
• Analyze immediately after sampling!
• The pH must be within the range 4 - 8.
Adjust, if necessary, with sodium hydroxide solution or sulfuric acid.
• Filter turbid samples.
7. Procedure
Pretreated sample 10 ml Pipette into a test tube.
(5 - 40 °C)
Reagent Cl2-1 1 level Add and shake vigorously until the reagent
blue microspoon is completely dissolved.
(in the cap of
the Cl2-1 bottle) Merck KGaA, 64271 Darmstadt, Germany,
Tel. +49(0)6151 72-2440
Leave to stand for 1 min (reaction time), then fill the sample into the cell, and measure www.analytical-test-kits.com
in the photometer. EMD Millipore Corporation, 290 Concord Road,
Billerica, MA 01821, USA, Tel. +1-978-715-4321
