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06 Cytoskeleton 2020 Notes

1. The document provides an overview of the cytoskeleton and motor proteins, including key terms like microtubules, actin, kinesin, dynein, and myosin. 2. It discusses how microtubules and actin filaments grow and shrink, and how motor proteins like kinesin, dynein, and myosin generate force by moving along these cytoskeletal fibers. 3. The document suggests three approaches to studying cytoskeletal and motor protein processes: using microscopes to view them, studying the effects of removing individual genes, and examining each component part separately.

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Wesley Chin
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91 views17 pages

06 Cytoskeleton 2020 Notes

1. The document provides an overview of the cytoskeleton and motor proteins, including key terms like microtubules, actin, kinesin, dynein, and myosin. 2. It discusses how microtubules and actin filaments grow and shrink, and how motor proteins like kinesin, dynein, and myosin generate force by moving along these cytoskeletal fibers. 3. The document suggests three approaches to studying cytoskeletal and motor protein processes: using microscopes to view them, studying the effects of removing individual genes, and examining each component part separately.

Uploaded by

Wesley Chin
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Cytoskeleton & Motors

This video provides the content for your class notes.


It moves quickly, so pause it often while you write.
You should have a complete set of notes to study from when you are done.

Bio Sci 93:


Thompson-Peer and Williams
Key Terms
• Cytoskeleton
• Motor proteins
• Microtubules
• Vs actin filaments
• Vs intermediate filaments
• Tubulin
• Actin
• Microtubule nucleators (centrosome and centriole)
• Cilia & flagella
• Kinesin
• Vs Dynein
• Vs Myosin
Cytoskeleton
2. _________
Lin et al, 2017

1. ______
3. __________

Bekkers, 2011 David Rogers, Vanderbilt, 1950s


Building the cytoskeleton
Building the cytoskeleton

Where is the + end?


Where is the – end?

10 µm
Building the cytoskeleton

Muroyama & Lechler, 2017


Building the cytoskeleton
Generating force: motor proteins

___

• MT
• ______
• ______
• Actin
• _______
Generating force: motor proteins
• MT: ___________

• Actin: _________
Generating force: motor proteins
Inner Life of the Cell
• [Link]

• 2:15 through 4:25


How could you study these processes?
Watch it Try each component part Remove one gene
at a time
_________ ________________
______________

[Link]
How could you study these processes?

50 µm

0.1 µm
10 µm
How could you study these processes?

Nucleus
Length Smallest
Most Small
of some Most bacteria
bacteria Proteins molecules
nerve plant
and Viruses
and
Human muscle Chicken Frog Human animal Mito- Ribo-
height cells egg egg egg cells chondrion somes Lipids Atoms

10 m 1m 0.1 m 1 cm 1 mm 100 µm 10 µm 1 µm 100 nm 10 nm 1 nm 0.1 nm


Required Reading
• Ch 6 A Tour of the Cell
• Biologists use microscopes and biochemistry to study cells

• The cytoskeleton is a network of fibers that organizes structures and


activities in the cell

• Extracellular components and connections between cells help


coordinate cellular activities

Reading these sections of your textbook is required to understand the topics covered in this Basics Lecture;
however topics in the reading that are not referenced during the lecture or class time will not be on the exam.
Prep for the Basics Quiz
Can you do the following?
• Describe the main functions of the cytoskeleton and
various kinds of fibers
• Discuss how actin and microtubules grow and shrink
• Identify the basic structure of a motor protein
• List differences between kinesin, dynein, and myosin
• Provide examples of motor proteins generating force in
cells
• Explain the 3 basic approaches in cell biology
Prep for the Exams
Will you be able to do the following types of things on an
exam?
• Compare and contrast the structures and functions of
cytoskeletal components
• Explain how the cytoskeleton works with motor
proteins to produce movement
• Determine the best type of microscopy to use to view
certain cellular structures

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