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Enzyme Activity: pH & Substrate Effects

This document describes a virtual enzyme lab simulation that allows students to investigate how substrate concentration and pH affect the rate of an enzyme-catalyzed reaction. The simulation measures the amount of product formed by the enzyme amylase breaking down starch into sugars over 1 minute at different substrate concentrations from 0.5g to 8.0g and pH levels from 3 to 11. Students are asked to collect data from the simulation and analyze the effects of substrate concentration and pH on the reaction rate.

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Samy Villafuerte
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159 views2 pages

Enzyme Activity: pH & Substrate Effects

This document describes a virtual enzyme lab simulation that allows students to investigate how substrate concentration and pH affect the rate of an enzyme-catalyzed reaction. The simulation measures the amount of product formed by the enzyme amylase breaking down starch into sugars over 1 minute at different substrate concentrations from 0.5g to 8.0g and pH levels from 3 to 11. Students are asked to collect data from the simulation and analyze the effects of substrate concentration and pH on the reaction rate.

Uploaded by

Samy Villafuerte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Enzyme Lab - Virtual [Link]

html

Name: ________________________________________

Enzyme Lab - Virtual


Site: [Link]

Overview: In this investigation, you will determine the effects


of substrate concentration and pH on the rate of an enzyme-
catalzyed reation.

Background:

Amylase is
an enzyme that catalyses the hydrolysis of starch into sugars.
It is present in the saliva of humans and some other mammals,
where it begins the chemical process of digestion. Foods that contain large amounts of starch, like bread, may
acquire a slightly sweet taste as they are chewed because amylase degrades that starch into sugar.

In the reaction shown, the enzyme is the amylase and the substrate is starch. The program measures the
amount of produce produced after the reaction has progressed for 1 minute, allowing you to compare rates of
reaction by looking at the amount of end product.

Procecure: The simulation allows you to change two variables: pH level and the amount of available substrate.
Data from the simulation can be viewed by clicking on the computer monitor.

*The simulation allows you to input data and print, but due to technical issues with browsers and networked
computers, it is preferable to copy the data onto this page.

Data: Collect data for each of the variables and complete the table below. The data collected is "number of
molecules of product formed per minute."

Amount of Substrate pH 3 pH 5 pH 7 pH 9 pH 11

0.5g

1.0g

2.0g

4.0g

8.0g

Analysis:

1. Use a graphing program like Exel or "Google sheets" to graph the data showing 5 separate lines for each pH
level. Attach this graph to this worksheet when you turn it in. To get started, you will be copying the data on the
table above to your spread sheet.

Identify the dependent and independent variables (hint: there are two independent variables you investigated,
but only one dependent variable.)

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Enzyme Lab - Virtual [Link]

2. Describe the relationship between substrate and the product. What happens when you increase the amount of
substrate? Predict what would happen if you used 20.g of substrate and explain your reasoning behind the
prediction.

3. Explain why the maximum initial reaction rate cannot be reached at low substrate concentrations.

4. What is the optimum pH level for this enzyme? How do you know? Make a prediction about what you think the
reaction rate would look like a pH = 2.

5. Enzymes function most efficiently at the temperature of a typical cell, which is 37 degrees Celsius. Increases
or decreases in temperature can significantly lower the reaction rate. What does this suggest about the
importance of temperature-regulating mechanisms in organisms? Explain.

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