ANALYSIS
A. ACROLEIN TEST.
Four samples in separate test tubes underwent the acrolein test, a test for the
identification of free glycerol or a compound containing glycerol. The following samples of
glycerol, coconut oil, lecithin and oleic acid tested positive as these samples produced a pungent
or burnt smell, and a black color after it was heated which indicated the presence of glycerol, and
therefore fat or lecithin. The samples, which are fats, shed molecules when reacted with KHSO4
or potassium bisulfate, a dehydrating agent which caused the lost of water of the samples. The
Potassium bisulfate soaked up the moisture that resulted to the formation of unsaturated aldehyde
from the glycerol portion of fats.
B. UNSATURATION TEST
The unsaturation test is used to detect presence of double bonds by adding Hubl’s
solution (dark red in color). Olive oil, oleic acid, coconut oil, and stearic acid were used as
samples in this part of the experiment. Olive oil had three-hundred and fifty drops of the Hubl’s
solution (350), oleic acid had three-hundred and forty drops (340), coconut oil had two-hundred
and twenty-eight (228) drops, and stearic acid had twenty-six (26) drops of the Hubl’s solution.
The number of drops corresponds to when the double bonds were detected. The more drop
added, the more unsaturated and multi-bonded the samples are, and in this test, olive oil proved
to be the most unsaturated out of the other three.
C. TEST FOR PHOSPHATE
Lecithin was used for this experiment. It has a burnt smell when incinerated in the
porcelain crucible. Added with water and was filtered. Also the (NH4)2MoO4 and HNO3 were
added. Direct heating was applied. There were precipitate but majority of it is white only small
particles of color yellow precipitate which indicates the presence of Phosphate in the experiment.
D. EMULSIFICATION TEST
Test tube 1 which has coconut oil and bile salt solution and test tube 2 with olive oil and
aqueous solution of lecithin are just the same especially when viewed in the microscope. The
presence of bubbles can be seen and also the bubbles are away from each other, hydrophobic.
While the Test tube 2 with coconut oil and H2O plus tiny crystal of Cholesterol were a bit
different still bubbles are away from each other but there were crystal like. Which can be seen in
the sketch in Table D.
E. LIEBERMAN-BURCHARD TEST
Two samples were tested in this experiment: bile salts and cholesterol with only one
sample testing positive as it turned into a deep green color after a few minutes. Cholesterol
produced a deep green product, a sulphonic acid (member of the class of organosulfur) derivative
of cholesterilene, which indicates presence of cholesterol. The deep dark green color it produced
was because of the hydroxyl group of cholesterol that reacted with the reagents, which are acetic
anhydride, and sulfuric acid, a reagent when added removes water molecule from C3 of
cholesterol molecule, and is oxidized to form 3-5 cholestadiens.
F. CARR-PRICE REACTION
Carr-Price test is used to detect presence of vitamin A. The reagent used in this test is
SbCl3 in CHCl3. The positive result is the formation of blue to green to gray to pink solution.
Vitamin A, cod liver oil, and olive oil were used as a sample in this experiment. However, in our
samples gave a negative test indicating that the sample does not contain Vitamin A. β-carotene is
a precursor to vitamin A which is highly conjugated and lipophilic.
G. MODIFIED FURTER-MEYER TEST
A bronze-red solution formed in this test indicating the presence of α-tocopherol. Only α-
tocopherol is recognized to meet human requirements.
CONCLUSION
Lipids show many physical and chemical properties. These properties were shown
in the products produced by the reactions in the tests with the exceptions of the tiny crystals of
cholesterol added with coconut oil in the Emulsification test, the bile salts in the Lieberman-
Burchard test, and the reaction in Carr-Price which all tested negative. Those products or
solutions that tested negative might have undergone an inaccurate process during the experiment
which resulted to the absence of the desired identity of the products.
REFERENCES
http://www.umb.edu.pl/photo/pliki/WL_jednostki/zaklad_biochemii_lekarskiej/pdf/bioche
mistry_workbook.pdf
http://fac.ksu.edu.sa/sites/default/files/Qualitative%20test%20of%20Lipids%20II.pdf
https://www.chem.wisc.edu/deptfiles/genchem/netorial/modules/biomolecules/modules/li
pids/lipid2.htm
http://www.scribd.com/doc/51367254/Acrolein-Test-and-Ester-Test-for-Lipids#scribd
