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Module 6 Worksheet-Updated

This document is a worksheet for a biological sciences class exercise on staining methods. It provides instructions for students to perform and record observations from Gram staining, endospore staining, and acid-fast staining of bacterial samples. The worksheet also includes discussion questions for each staining procedure and two extension questions that apply the staining concepts to diagnosing a strep throat infection and selecting an appropriate antibiotic treatment.

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Juan Paolo Mendez
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94 views3 pages

Module 6 Worksheet-Updated

This document is a worksheet for a biological sciences class exercise on staining methods. It provides instructions for students to perform and record observations from Gram staining, endospore staining, and acid-fast staining of bacterial samples. The worksheet also includes discussion questions for each staining procedure and two extension questions that apply the staining concepts to diagnosing a strep throat infection and selecting an appropriate antibiotic treatment.

Uploaded by

Juan Paolo Mendez
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

University of Santo Tomas

College of Science
Department of Biological Sciences

WORKSHEET - EXERCISE 6
Staining Methods

Name of student (Last name first) Date: ________________


Section: _____________
__________________________________________ Group No. __________

--------------------------------------------------------------------------------------------------------------------------------

I. RESULTS AND OBSERVATIONS


*Use colored pencils in your drawings*

Directions: Give examples of bacteria (Gram-positive and/or Gram-negative) and describe their
morphological characteristics (in terms of shape, arrangement, and color) after the completion of the
staining procedure.

Gram Stain:

Endospore Stain:

Endospore Present
or Absent

Endospore (Present or Absent

Acid Fast Stain:


University of Santo Tomas
College of Science
Department of Biological Sciences

Acid Fast Bacilli


seen
Or
Non acid fast

II. DISCUSSION

Directions: Write all of your observations and analyses/explanations to the questions given for each
procedure.

1. Bacterial Smear

2. Simple Stain

3. Gram Stain

4. Endospore Stain

5. Acid-Fast Stain

Extension questions or case study for real-world application


University of Santo Tomas
College of Science
Department of Biological Sciences

1. You are doing your infectious disease rotation as a medical intern and come upon a patient
that is suspected to have a strep throat infection. One of the tests to be done on samples
(throat swab, sputum) taken from the patient is a Gram stain. What morphology, cell
arrangement and Gram reaction are you most likely to see?

2. Assuming that the patient is positive for a strep infection, the medical consultant then asks
you to choose what antibiotic the patient should be given. You are told to choose between:
a) erythromycin; b) tetracycline; c) amoxicillin; d) isoniazid and rifampicin. Which
antibiotic(s) will you choose? Explain this in relation to what you know about bacterial cell
structure and the Gram stain reaction.

III. REFERENCES (Follow the APA format)

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