specification sheet 7300 Real-Time PCR System

Applied Biosystems
7300 Real-Time PCR System
A Real Affordable Approach to Real-Time PCR

• Four-color detection provides

the flexibility to perform a variety
of applications, including gene
expression analysis, pathogen
quantitation, SNP genotyping,
isothermal, and plus/minus assays
that utilize internal positive controls
• Powerful, versatile software includes
plate setup wizards that guide you
through experimental setup; advanced
data-viewing capabilities including
data sorting and custom graphing
options, simple export to PowerPoint,
and automated analysis tools that
make data processing simple and
straightforward
• Precisionoptics and a chargecoupled
device (CCD) camera, combined with The Applied Biosystems Advantage Quantitative results are available
a sophisticated multicomponenting Built on over 10 years of real-time immediately upon completion of PCR,
algorithm, provide highly accurate, expertise, the Applied Biosystems with no need to run gels, purify PCR
reproducible, and reliable results 7300 Real-Time PCR System is an products, or perform any post-PCR
• Latest
generation, Peltier- affordable platform for the detection and manipulations. Real-time PCR runs are
based, thermal cycling system quantification of nucleic acid sequences completed in under two hours, using
accommodates both standard that will not compromise your data 96-well plates and tubes (individual or
96-well plates and 0.2 mL tubes quality or dye choice flexibility. The 8-strip), with a supported volume range
7300 Real-Time PCR System combines of 20 – 100 μL. Compared with manual
thermal cycling, fluorescence detection, PCR quantitation techniques such as
and application-specific software to Northern blotting or RNase protection
measure the cycle-by-cycle accumulation assays, real-time PCR offers enormous
of PCR products in a single-tube, time-savings, greater sensitivity, superior
homogeneous reaction. precision, and a larger dynamic range.
This high-quality platform from the leader
in real-time PCR systems provides data
you can trust at a price you can afford.
Real-Time PCR Applications
The 7300 system supports many Instrument Specifications

real-time quantitative PCR applications
Thermal cycling system Peltier-based, 96-well block
including gene expression analysis using
relative quantitation (RQ) assays, and Sample Ramp Rate +/-1.1°C/sec
absolute quantitation using standard Peak Block Ramp Rate 1.6°C/sec
curves. In addition, the system allows
for qualitative, post-PCR detection of Temperature Range 4°C – 100°C

nucleic acids for allelic discrimination Temperature Accuracy ±0.25°C of setpoint/display temperature measured
(SNP genotyping) assays and plus/minus at 3 minutes after clock start
assays that use internal positive controls. Temperature Uniformity ±0.50°C, 30 seconds after clock start
Fluorescence Detection Optical system Single excitation, four emission filters,
All sample wells in the 7300 system and CCD camera
are illuminated with a tungsten-
Calibrated Dyes at Installation SYBR ® Green I, FAM TM , VIC ®, JOE TM , NED TM ,
halogen lamp. Fluorescence emission
TAMRATM , ROXTM
is detected through four filters on to a
CCD camera. The emission filters are Passive Reference Dyes ROXTM or any calibrated dye.
optimized for use with FAMTM/SYBR®
Data Collection Data collected in all 4 filters for all wells regardless
Green I, VIC®/JOETM, NEDTM, TAMRATM of plate setup. Plate setup may be altered after
and ROXTM fluorescent dyes. run completes.
Sequence Detection Software Quantitative PCR run time < 1 hour 50 minutes
Instrument software for the Applied
Supported Volumes 20 – 100 μL
Biosystems 7300 Real-Time PCR
System runs on the Windows XP Supported Consumables • Standard optical 96-well plates
operating system and provides • 8-strip 0.2mL tubes
instrument control, data collection, • 0.2mL tubes

• Optical adhesive covers

and data analysis. Powerful and user-
• Optical flat caps
friendly, sequence detection software
includes the following features:
• P
 late
setup wizards for easy
experimental design, even with
complex multicolor assays • A
 utomated SNP genotype calling • V
 ersatile system can be used as
• R
 eal-time
capability with intuitive graphical a plate reader or a regular thermal
monitoring of amplification
output and quality-value assignment cycler if needed
growth curves enables you to view
run progress • S
 imple dissociation curve data • S
 implecorrection of plate setup
• A
 uto-baseline
collection and viewing mistakes without losing collected data
and auto-threshold
for simplified data analysis • T
 ool tips for easy identification Computer Specifications
• A
 bsolute
of sample wells when viewing Applied Biosystems supplies a DellTM
quantitation of nucleic
amplification curves or SNP Business Line computer (notebook or
acid targets with the ability to
genotyping plots tower) for use with the 7300 system. For
simultaneously analyze multiple
the latest computer specifications, please
standard curves on a single plate • L
 amp-life monitoring and instrument
visit the Applied Biosystems Web site at
• O
 ptional
diagnostics provide confidence in
relative quantitation (RQ) www.appliedbiosystems.com.
your instrument’s performance
study software with powerful data
Installation Specifications
viewing capabilities allowing • D
 ata sorting and filtering for easy
Using the TaqMan® RNase P Instrument
the simultaneous analysis of up viewing and reporting, along with
Verification Plate, the 7300 system can
to ten 96-well plates containing customizable graphs for targets or
distinguish between samples containing
gene expression data samples of interest
5,000 and 10,000 template copies with
a confidence level of 99.7%.
Demonstrated Performance TaqMan® Assays—optimized for use on the
The 7300 system has been
7300 Real-Time PCR System
demonstrated to achieve the
following performance targets: Applied Biosystems provides the largest selection of gold standard TaqMan Assays
for gene expression and genotyping applications in a variety of format options.
• 9
 logs of linear dynamic range
Over 800,000 inventoried TaqMan Gene Expression Assays covering ten species;
• D
 etectionof 10 starting copies of a
Custom TaqMan Gene Expression Assays for any genome and any species; and
DNA template in a 50 μL reaction for
TaqMan MicroRNA Assays are available for miRNA quantitation. Learn more about
a single reporter TaqMan assay with
these and other TaqMan Gene Expression Assay products at www.allgenes.com.
a confidence level of 99.7%
Over 4.5 million made-to-order TaqMan SNP Genotyping Assays for human and
Reagents and Disposables
mouse; Custom TaqMan SNP Genotyping Assays for any SNP and any species; and
A complete line of reagents including
TaqMan Drug Metabolism Genotyping Assays are for high value polymorphisms.
TaqMan® Universal PCR Master Mixes
Learn more about these and other TaqMan SNP Genotyping Assay products at
and SYBR® Green I Master Mixes, and
www.allsnps.com.
disposables including tubes and 96-well
plates are available for use with the
7300 system. Instrument and Computer Dimensions

TaqMan Genomic Assays

Dimension 7300 System Notebook Tower
Applied Biosystems provides
preformulated, ready-to-use, Width 34 cm (13.39 in.) 32 cm (12.4 in.) 18 cm (7.1 in.)

quality-tested, 5’ nuclease TaqMan Depth 45 cm (17.72 in.) 26 cm (10.1 in.) 45 cm (17.6 in.)
probe-based assays for use with
Height 49 cm (19.29 in.) 3 cm (1.2 in. closed) 42 cm (16.7 in.)
the 7300 system (see TaqMan
Assays section). Weight 29 kg (64 lb) 2.1 kg (4.7 lb) 32 kg (70 lb)

Service and Warranty

Purchase of the instrument includes
a one-year limited warranty on parts
and labor, plus an installation package
that includes setup and calibration of
the instrument from our highly trained
Service Support team.

Support
Applied Biosystems technical specialists
and scientists provide worldwide
applications support and service.
oRDeRinG infoRMation

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NOTICE TO PURCHASER:
The Applied Biosystems 7300 Real-Time PCR System is a real-time thermal cycler covered by US patents and claims in non-US counterparts. No right is conveyed expressly, by
implication or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5’ nuclease methods. Further information on purchasing
licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
© 2008, 2010 Applied Biosystems, Inc. All rights reserved. Applied Biosystems, AB (Design), SYBR and VIC are registered trademarks and FAM, JOE, NED, ROX, and TAMRA
are trademarks of Applied Biosystems, Inc. or its subsidiaries in the US and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Dell
is a trademark of Dell Corporation. Microsoft and Windows XP are registered trademarks of Microsoft Corporation. Pentium is a registered trademark of Intel Corporation. All
other trademarks are the property of their respective owners.
Printed in the USA, 09/10, Publication 117SP04-06

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