PART 1: SALIVARY AMYLASE ACTIVITY
INTRODUCTION
Starch, a complex carbohydrate polymer, can be found in grains like wheat, corn and oats.
Starch, which is a polymer made from thousands of sugar molecules bonded together, stores
energy for plants. Digestion of starch normally begins in the mouth of humans where an
enzyme catalyst, salivary amylase, is secreted to speed up the breaking of starch into its
monomer sugars.
Enzymes are specific. Each chemical reaction uses a different enzyme that has a specific
active site shaped perfectly to fit the specific substrate/reactant. The substrate fits into the
active site of the enzyme allowing for bond breaking or making to catalyze the reaction.
Enzymes are denatured when excessively heated in that they unravel and their shape is
permanently altered making them ineffective. Digestion of starch normally begins in the
mouth of humans where an enzyme, salivary amylase, is secreted, it catalyse the
break-up of the starch (Cecie Star, 2014).
OBJECTIVE
To observe the function of enzyme amylase in saliva that will give sweet taste to cracker.
MATERIAL
Saltine cracker
PROCEDURE
1. One saltine cracker was placed on tongue without swallowing.
2. The saltine cracker was chewed for about 3 to 5 minutes - it’s a long time. Make sure
DO NOT swallow.
3. Observation was make by describe the sweet taste of saltine cracker after a few
minute.
RESULT
Time (min) Taste
0 Nothing sweetness detect
3 Slightly sweetness detect
5 Strongly Sweetness detect
DISCUSSION
Starch is a long polymer of glucose molecules. There are three forms of starch- amylase,
amylopectin and animal starch which glycogen. Amylase is the form that found in the
crackers. Amylase is a linear chain of 200-2000 glucose sub units that will bonded together
that forms a long helical structure. In this experiment when the cracker is start chewed up, so
the salivary amylase start breaking down the cracker into shorter glucose polymers. Some
will converted into individual glucose molecules that may impart a sweetness taste. However
as we know glucose is not particularly sweet, but the fructose very similar in structure to
glucose so that it is so much sweeter. In table sugar- sucrose is a disaccharide of glucose and
fructose, which will give sweet taste to the cracker. So that cracker will give sweet taste after
a 5 minutes chewed it up because in mouth saliva has provides enough amylase to digest all
of the starch that break down starch in simple sugar like glucose (Jiby, 2016).
CONCLUSION
In the conclusion we can assume that the sweetness of the cracker was by helping of enzyme
amylase that found in the saliva. This salivary amylase was break the cracker into small
molecules and into shorter glucose polymer which is glucose is a simple sugar.
REFERENCE
Cecie S. & Beverly M. (2014). Human biology. Canada: Nelson Education.
Jiby J. M. (2016). Behaviour of Salivary Amylase in Various Reaction Environments. USA:
GRIN publishing.