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Flotation Bath

This document discusses several instruments and processes used in tissue sample preparation for histological examination, including a flotation bath, cryostat, microtome, tissue processing, embedding, sectioning, staining, and mounting. Key steps involve fixation, grossing, processing, embedding tissues in paraffin blocks, sectioning blocks with a microtome, mounting sections onto slides, and staining before examination under a microscope.

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nurul fatinajihah
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503 views3 pages

Flotation Bath

This document discusses several instruments and processes used in tissue sample preparation for histological examination, including a flotation bath, cryostat, microtome, tissue processing, embedding, sectioning, staining, and mounting. Key steps involve fixation, grossing, processing, embedding tissues in paraffin blocks, sectioning blocks with a microtome, mounting sections onto slides, and staining before examination under a microscope.

Uploaded by

nurul fatinajihah
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

FLOTATION BATH:

- [Link]
- [Link]
- [Link]
q=flotation+bath&espv=2&biw=1366&bih=667&tbm=isch&tbo=u&source=univ&sa=X&ved=
0ahUKEwioiKyg3PDNAhVJtY8KHVb1BFkQsAQIGw
- [Link]
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CRYOSTAT:
- [Link]
- [Link]

MICROTOME:
- [Link]
[Link]
- [Link]

TISSUE PROCESSING:
- [Link] ?
- [Link]
q=process+of+tissue+processing&espv=2&biw=1366&bih=667&tbm=isch&tbo=u&source=u
niv&sa=X&ved=0ahUKEwiuhJ-R1vXNAhVJLI8KHTf-BtMQsAQIPQ
- [Link]
processing/

TISSUE EMBEDDING:
- [Link]
histology/content-section-2.2
- [Link]
case=normal&funcfor=course&funcmod=information&courseid=CSI102&lv=1&refid=992335
&ccshowid=1119839&fcid=755007&nmfile=L6_Tissue
%[Link]&dlfile=/download/2016625096/L6_Tissue%[Link]
- [Link]
q=process+of+tissue+processing&espv=2&biw=1366&bih=667&tbm=isch&tbo=u&source=u
niv&sa=X&ved=0ahUKEwiuhJ-R1vXNAhVJLI8KHTf-
BtMQsAQIPQ#tbm=isch&q=tissue+embedding
-
Chapter Content Page
TITLE PAGE
ABSTRACT
TABLE OF CONTENTS
LIST OF TABLES
LIST OF FIGURES
1.0 INTRODUCTION
1.1 Overview
1.2 Objective
1.2.1 General Objective
1.2.2 Specific Objective
2.0 MATERIALS AND METHODS
2.1 Study Venue
2.2 Materials and Equipments
2.1.1 Materials
2.2.2 Equipment
2.3 Calculation for reagent preparation
2.4 Tissue fixation procedure
2.5 Tissue grossing procedure
2.6 Tissue processing procedure
2.7 Tissue embedding procedure
2.8 Tissue sectioning procedure
2.9 Tissue fishing procedure
2.10 Tissue staining procedure
2.11 Tissue mounting procedure
2.12 Tissue observation procedure
3.0 RESULTS
3.1 Sample collection
3.2 The tissue section slide produced
3.3 The observation of tissue section under microscopic
4.0 DISCUSSION
5.0 CONCLUSION AND RECOMMENDATIONS
REFERENCES
APPENDIXES

Table 2.3 Calculation of fixative reagent


Table 2.6 Tissue processing procedure
Table 2.10 Tissue staining procedure

Figure 2.5 Tissue grossing process


Figure 2.7 Tissue embedding process
Figure 2.8 Tissue sectioning process
Figure 2.11 Tissue mounting process
Figure 2.12 Tissue observation
Figure 3.1 3.1. Tissue blocks
1
3.1. Tissues after grossing
2
Figure 3.2 3.2. Tissue sections after sectioning process
1
3.2. Tissue sections after staining process
2
Figure 3.3 Observation under compound microscopic

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