R-Biopharm AG
Analysis of nitrofuran antibiotics in food
RIDASCREEN® Nitrofuran ELISAs for screening of nitrofuran metabolites
• Full product range: AHD, AMOZ, AOZ, SEM
• Compliant to Minimum Required Performance Limits
• Combined sample preparation for shrimp and fish
• Automation of test implementation
R-Biopharm – for the safety of your analysis.
R-Biopharm AG
Introduction
The nitrofuran antibiotics Nitrofurantoin, Non-compliance with this legislation may lead to
Furaltadone, Furazolidone and Nitrofurazone severe penalties e.g. during export. Since the parent
are synthetic broad-spectrum antibiotics, which drugs are rapidly metabolized after administration,
are frequently used in animal production for the analysis of nitrofuran drugs is based on the
their excellent antibacterial and pharmacokinetic detection of the tissue bound metabolites AHD,
properties. Due to their potential carcinogenic AMOZ, AOZ and SEM. For this reason R-Biopharm
and mutagenic characteristics, their application offers 4 test kits for the quantitatve screening of
in animals used for food production has been nitrofuran metabolites in food.
prohibited with introduction of zero-tolerance levels.
Specifications
RIDASCREEN® Nitrofuran
AHD AMOZ AOZ SEM
Format Microtiter plate with 96 wells (12 strips with 8 wells each)
double determination is recommended, sufficient for 42 samples
Standards 0, 100, 300, 900, 0, 25, 50, 100, 0, 100, 300, 900,
[ng/l = ppt] 2700, 8100 200, 400 2700, 8100
Nitrobenzaldehyde included
Spiking solution Art. No. R3796 Art. No. R3799 Art. No. R3798 Art. No. R3797
Spiking procedures available on request
Sample preparation Part 1 1.5 h
Derivatization 3h
Part 2 1.5 h
Test implementation 1 h 15 min
Specificity [%] NP-AHD 100 <1 <1 <1
(in buffer system) NP-AMOZ <1 100 <1 <1
NP-AOZ <1 <1 100 <1
NP-SEM <1 <1 <1 100
Minimum Required All matrices 1000
Performance Limit (MRPL)
[ng/kg=ppt]
Limit of detection Shrimp* 200 200 50 300
[ng/kg=ppt] Beef 200 100 300
(corresponding to the Pork 200 100 300
standard substance)
Poultry 200 100 400
Fish* 80 200 50 360
Whole egg 200 100
Liver 200 100
Milk 50
Honey 100
Recovery [%] Shrimp* 95 80 - 100 85 - 100 98
(corresponding to the Beef 70 - 90 80 - 100 94
standard substance) Pork 70 - 80 75 - 100 92
Poultry 70 - 100 80 - 100 110
Fish* 82 80 - 100 85 - 100 98
Whole egg 80 - 100 90 -100
Liver 70 - 90 80 - 100
Milk 80 - 100
Honey 95 95
* Multiplex sample preparation protocol available
5 in 1 sample preparation of nitrofurans and chloramphenicol in
shrimp followed by manual pipetting or automated analysis by
ChemWell® analyzer
1 Aquaculture
Parent Drug Metabolite in the shrimp
• Nitrofurantoin AHD
• Furaltadone AMOZ
• Furazolidone AOZ
• Nitrofurazone SEM
• Chloramphenicol CAP
2 Sample preparation
1.5 h • Homogenization (eventual Spiking with RIDA® Spiking Solution)
3h
• Hydrolysis & derivatization of metabolites into nitrophenyl
(NP-) derivates
1.5 h • Extraction
• Evaporation
• Reconstitution
Sample Extract contains NP-AHD, NP-AMOZ, NP-AOZ, NP-SEM, CAP
3 Analysis (manual or automated)
Manual analysis • Add standard/sample
• Add enzyme conjugate
• Add antibody
1 h Incubation
• Washing
ChemWell® • Add substrate chromogen
15 min Incubation
• Add stop solution
• Measurement
4 Data evaluation Evaluation of results with RIDA® SOFT WIN (Art. No. Z9996)
R-Biopharm AG
Test systems for the detection of nitrofuran metabolites and
chloramphenicol
Product Description No. of tests/amount Art. No.
ELISA, Microtiter plates
RIDASCREEN® Nitrofuran Enzyme immunoassay for quantitative analysis of AOZ in 96 determinations R3703
(AOZ) shrimp, meat (poultry, pork, beef), liver, fish, whole egg, milk, Incubation time:
honey 1 hr 15 min
Detection limits: shrimp, fish, milk 50 ppt, meat/liver/whole
egg/honey 100 ppt
RIDA® Nitrofuran (AOZ) 20 ng/ml 1 ml R3798
Spiking Solution
RIDASCREEN® Nitrofuran Enzyme immunoassay for quantitative analysis of AMOZ in 96 determinations R3711
(AMOZ) shrimp, meat (poultry, pork, beef), liver, fish, whole egg Incubation time:
Detection limit: 200 ppt 1 hr 15 min
RIDA® Nitrofuran (AMOZ) 20 ng/ml 1 ml R3799
Spiking Solution
RIDASCREEN® Nitrofuran Enzyme immunoassay for quantitative analysis of AHD in 96 determinations R3713
(AHD) shrimp Incubation time:
Detection limit: shrimp 200 ppt 1 hr 15 min
RIDA® Nitrofuran (AHD) 20 ng/ml 1 ml R3796
Spiking Solution
RIDASCREEN® Nitrofuran Enzyme immunoassay for quantitative analysis of SEM in meat 96 determinations R3715
(SEM) (poultry, pork, beef), fish, shrimp Incubation time:
Detection limits: beef, pork, shrimp 300 ppt, fish 360 ppt, 1 hr 15 min
poultry 400 ppt
RIDA® Nitrofuran (SEM) 20 ng/ml 1 ml R3797
Spiking Solution
RIDASCREEN® Enzyme immunoassay for quantitative analysis of 96 determinations R1505
Chloramphenicol chloramphenicol in different matrices* Incubation time: 1 hr
Detection limits: depending on matrix 5 - 50 ppt 15 min
RIDA® Chloramphenicol 50 ng/ml 1 ml R1599
Spiking Solution
For further details and information visit our website www.r-biopharm.com , contact your local distributor
or our Internationals Sales Office.
R-Biopharm contacts:
International Sales: Order Department:
Phone: +49 (0) 61 51 - 81 02-0 Phone: +49 (0) 61 51 - 81 02-0
Fax: +49 (0) 61 51 - 81 02-40 Fax: +49 (0) 61 51 - 81 02-20
E-mail:
[email protected] E-mail:
[email protected] 01/2016
R-Biopharm AG • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: [email protected] • www.r-biopharm.com
