SONICATOR

Ultrasonic Liquid Processors

Index
How Does a Sonicator Work..................................................................................... 2-3
Direct vs. Indirect Sonication....................................................................................... 3

Q700 Sonicator............................................................................................................ 4-5

Q500 Sonicator............................................................................................................ 6

Accessories for the Q700 and Q500 ..................................................................... 7-17

Direct Horn Options.................................................................................................... 7-9
Standard Probes .................................................................................................. 7
Replacement Tips and Microtips ......................................................................... 8
Extenders, Boosters, and High Gain Horns ......................................................... 9
High Throughput Horns ............................................................................................. 10
4 Tip Horn, 24 Tip Horn, Dual Horn
Sound Enclosure ....................................................................................................... 11
Flocells........................................................................................................................ 12-13
Indirect Horn Options................................................................................................. 14 -15
Cup Horn and Microplate Horn
Chiller.......................................................................................................................... 16
General Accessories .................................................................................................. 17

Q125 Sonicator............................................................................................................ 18
Q125 Accessories ...................................................................................................... 19 

Q55 Sonicator .............................................................................................................. 20

CoolRacks .................................................................................................................... 21

Q1375 Sonicator ......................................................................................................... 22

Q1375 Accessories ................................................................................................... 23

Frequently Asked Questions .................................................................................... 24

© 2015 Specifications subject to change without notice.

1
How Does a Sonicator Work?

Generator Converter Probe/Horn

A Sonicator system is comprised of 3 major In liquid, the rapid vibration of the tip causes
components: Generator, Converter and cavitation, the formation and violent collapse
Horn (also known as a probe). of microscopic bubbles. The collapse of
thousands of cavitation bubbles releases
The ultrasonic electronic Generator transforms tremendous energy in the cavitation field.
AC line power to high frequency electrical energy. Objects and surfaces within the cavitation
The generator features a keypad or buttons which field are “processed.” By increasing the
allow the user to control the sonication parameters. amplitude setting, cavitation intensity
within the sample is also increased.
The generator provides high voltage pulses
of energy at a frequency of 20 kHz that drives The ultrasonic frequency is fixed at 20 kHz
a piezoelectric Converter. The converter is and does not fluctuate during sonication.
a cylindrical device which is connected to the The Sonicator continuously calibrates itself
generator by a high voltage cable. The converter to ensure consistent output. Processing
transforms electrical energy to mechanical time and amplitude (intensity) are adjustable.
vibration due to the characteristics of the Sonication generates heat so the system can
internal piezoelectric crystals. be pulsed on/off to reduce this effect. If heating
of the sample is not an issue, the continuous
The vibration is amplified and transmitted sonication option can be selected.
down the length of the Probe/Horn. Probes
have threaded ends and attach to the converter. To ensure a positive outcome, it is important
During operation, the probe’s tip longitudinally to select the appropriate generator and probe
expands and contracts. Amplitude is the distance to match the volume, viscosity and other
the tip travels and is dependent on the amplitude parameters of each particular application.
setting selected by the user. Please consult with a Sonicator product
specialist for help making the optimum choices.

2
Direct vs. Indirect
Sonication Methods
DIRECT Sonication (inserting a probe directly
into a sample vessel) is the most common way
to process a sample. Energy is transmitted from
the probe directly into the sample with high
intensity and the sample is processed quickly.

The diameter of the probe’s tip dictates the

liquid volume that can be effectively processed.
Smaller tip diameters (Microtip probes) deliver
high intensity sonication and the energy is
focused within a small, concentrated area.
Larger tip diameters can process larger volumes,
but offer lower intensity. Boosters and High Gain
horns can be used to increase the output of
large diameter probes. Probes are offered with
either replaceable or solid tips and are made
from titanium.

INDIRECT Sonication eliminates the need for

a probe to come in contact with your sample.
This technique is often described as a high
intensity ultrasonic bath. The ultrasonic energy
is transmitted from the horn, up through the
water and into a vessel or multiple sample tubes.

Indirect sonication is most effective for very

small samples because foaming and sample
loss are eliminated. Pathogenic or sterile
samples are ideal for this method because
aerosols and cross contamination are prevented.
The Cup Horn and Microplate Horn deliver
indirect sonication and are ideal for many
high throughput applications.

3
Q700 Sonicator
The new Q700 is the most technologically advanced sonicator
available today. A state-of-the-art touch screen interface
offers intuitive control and provides a user-friendly experience.
The most important feature of a Sonicator is reproducibility.
Improved internal circuitry guarantees more efficient operation,
sample-to-sample consistency and most importantly,
a reliable end result.

The Q700 is the only sonicator on the market that offers full amplitude control
from 1-100%. This enables greater control of the probe’s intensity, helping
to pinpoint the optimum settings for efficient sample processing. We have
increased maximum power output to 700 watts making the system more
durable and capable of handling even larger samples if necessary. Our
new display, design improvements and added accessories make this
the most sophisticated and versatile Sonicator available today.

Stand sold separately.

4
FEATURES:

FULL AMPLITUDE CONTROL RUN MULTIPLE PROGRAMS TOUCH SCREEN CONTROL

Amplitude (intensity) is controlled IN SEQUENCE A large, color LCD screen clearly
from 1-100% giving a greater Multiple programs can be run in displays all operating parameters
degree of resolution and the ability sequence. For example, the unit can and options. Intuitively and quickly
to pinpoint the amplitude needed to be programmed to sonicate at 50% access any of the sonicator’s
effectively process your sample. amplitude for 5 minutes, shut off
functions with a simple touch.
for 2 minutes and re-start at 25%
PROGRAMMABILITY amplitude for 10 minutes. Up to 5
Parameters including processing programs can be run in succession.
times, pulse on/off and amplitude
can be saved to memory and run TOTAL ENERGY OUTPUT DISPLAY
by the touch of a button. Energy delivered to the probe is
displayed in both Watts and Joules.
PULSE MODE
Adjustable pulse On and Off times to AUTO TUNING
reduce the heat gain in temperature The Sonicator digitally tracks
sensitive samples. frequency changes in the converter
/ tip assembly caused by load and
TEMPERATURE MONITORING temperature changes and maintains
An optional temperature probe is electrical efficiency at all times.
available for those customers who Manual tuning is unnecessary.
wish to monitor the temperature of
their sample. If the temperature limit OVERLOAD PROTECTION
is reached, sonication shuts down to The unit is equipped with fault
prevent overheating. detection circuitry to shut down
sonication in the event that a
RoHS COMPLIANT fault occurs.
All Qsonica equipment is built
lead free.

PART NO. Q700 INCLUDES: TECHNICAL SPECIFICATIONS:

• Generator Power Rating: 700 watts

Frequency: 20 kHz
• Converter
Programmability: 10 memories plus sequencing
• 1/2" (13 mm) diameter probe
Programmable Timer: 72 hours
• Power cable
Adjustable Pulse On/Off: 1 second to 24 hours
• Converter cable Dimensions (W x L x H): 8 x 15.25 x 8.5 in. ( 203 x 387 x 216 mm)
• Wrench set Voltage: 110V, 50/60 Hz
Specify desired voltage for export.

5
 Q500 Sonicator
The Q500 is a powerful ultrasonic
processor featuring programmable
operation and a digital display of
operating parameters. Popular
applications include nanoparticle
dispersion, creating emulsions,
cell lysis and homogenization.

Adjustable pulse On and Off times can be

programmed from 1 second to 1 minute. Total
programming has a maximum setting of 10 hours.
A wide variety of probes and accessories are
available to handle virtually any application. Stand sold separately.
FEATURES:
Programmable operation
Set time and amplitude for hands free operation
Pulse mode
Prevent heat buildup in temperature sensitive samples
ULTRASONIC
START/STOP Digital amplitude / intensity control
Output intensity can be set from 20-100%
PROCESSING POWER
TIMER ON/OFF Elapsed time indicator
Displays duration of sonication
PULSE/REST AMPLITUDE Display of wattage and joules
ADJUSTMENT Real-time energy monitoring
AMPLITUDE Overload protection
Prevents damage to circuitry if a fault occurs
RoHS compliant
Uses lead free components

PART NO. Q500 INCLUDES: TECHNICAL SPECIFICATIONS:

• Generator Power Rating: 500 watts
• Converter Frequency: 20 kHz
• 1/2" (13 mm) diameter probe Programmable Timer: 10 hours
• Power cable Adjustable Pulse On/Off: 1 second to 1 minute
• Converter cable Dimensions (W x L x H ) : 8 x 5.25 x 8.5 in. ( 203 x 387 x 216 mm)
• Wrench set Voltage: 110V, 50/60Hz
Specify desired voltage for export.

6
 Q700 / Q500 Accessories Direct Horn Options

Horns (also known as probes) are made from titanium

and machined to specific sizes and shapes. When driven
at their resonant frequency, they expand and contract
longitudinally. This mechanical vibration is amplified and
transmitted down the length of the probe. In liquid, the
probe causes cavitation which constitutes the main
mechanism for sample processing.

Choosing the appropriate horn is extremely important.

The sample volume to be processed is directly related to
the tip diameter. Smaller tip diameters (Microtip probes)
deliver high intensity sonication, but the energy is focused
within a small, concentrated area. Larger tip diameters can
process larger volumes, but offer lower intensity. Probes
are offered with replaceable or solid tips.

Probe tips will pit or erode over time and require

replacement. Replaceable tip probes are used with
aqueous samples only. In addition to aqueous samples,
Solid probes can be used with organic solvents, alcohols
and low surface tension liquids. Contact Qsonica with
questions regarding proper tip selection.

Standard Probes

Processing Amplitude
Part # Type of Tip Volume Tip Diameter (microns)

4220 Replaceable Tip 20-250 ml 1/2" (13 mm) 120 μm

4219 Solid Tip 20-250 ml 1/2" (13 mm) 120 μm

4207 Replaceable Tip 50-500 ml 3/4" (19 mm) 60 μm

4208 Solid Tip 50-500 ml 3/4" (19 mm) 60 μm

4210 Replaceable Tip 100-1,000 ml 1" (25 mm) 30 μm

4209 Solid Tip 100-1,000 ml 1" (25 mm) 30 μm

Note: All amplitude values are measured at 100% output.

Replaceable Solid

7
 Q700 / Q500 Accessories Direct Horn Options

Part # Tip Diameter For Use With

Replacement Tips 4406 1/2" (13 mm) #4220
for Standard Probes
4407 3/4" (19 mm) #4207
Standard ½", ¾" and 1" horns 4408 1" (25 mm) #4210
have replaceable tips. During
normal use, tips erode and
become less effective over time.
These worn tips can be easily
removed and replaced.
New Tip Worn Tip

Processing Amplitude
Microtip Probes Part # Volume* Tip Diameter (microns)
4417 0.2-5 ml 1/16" (2 mm) 320 μm
Microtips are thin, CONVERTER
high intensity probes 4418 1-15 ml 1/8" (3 mm) 380 μm
which are designed
4420 5-50 ml 1/4" (6 mm) 200 μm
for processing small
sample volumes. * The recommended processing volume range
Microtips screw into is application specific. For example, samples
containing surfactants foam easily and may require
the threaded end larger volumes for effective sonication. Contact
of the standard #4220 Qsonica for application assistance.
1/2” PROBE
½" probe (#4220).

#4418
MICROTIP

Coupler with Stepped Microtip

Coupler —
­ # 4421 Stepped Microtip — Part # 4422
The stepped microtip and coupler assembly is a low
Processing Amplitude
intensity option which can be used to process small Part # Volume Tip Diameter (microns)
volumes that do not require high power. The probe
4422* 0.5-15 ml 1/8″ (3 mm) 200 μm
tip remains 1/8” in diameter for 48mm. This 2-piece
assembly attaches directly to the converter. 4421 Coupler - *required for use of a Stepped Microtip

8
 Q700 / Q500 Accessories Direct Horn Options

Extenders
Standard probes may Part # Type of Tip Length Tip Diameter
not be long enough to
406HW Solid Tip 5" 1/2" (13 mm)
fit down into certain
406HWT Replaceable Tip 5" 1/2" (13 mm)
long necked vessels.
Extender probes attach 407HW Solid Tip 5" 3/4" (19 mm)
to standard horns of 407HWT Replaceable Tip 5" 3/4" (19 mm)
the same tip diameter 408HW Solid Tip 5" 1" (25 mm)
and extend the length 408HWT Replaceable Tip 5" 1" (25 mm)
of the horn assembly.
407FW Solid Tip 10" 3/4" (19 mm)
Extenders are available in
5" and 10" lengths with 407FWT Replaceable Tip 10" 3/4" (19 mm)

either solid, or replaceable tips. 408FW Solid Tip 10" 1" (25 mm)
408FWT Replaceable Tip 10" 1" (25 mm)
Extenders offer the same processing volume and
amplitude of their corresponding standard horn.

Boosters Booster horns increase the intensity of standard ¾" and 1" horns.
Boosters attach between the converter and horn to increase
amplitude by the gain ratio indicated below.

Part # For Use With Gain Ratio

4121 3/4" (19 mm) and 1" (25 mm) Probes 2 to 1

High Gain Horns High gain horns (also known as high intensity horns) offer double
the amplitude of standard ¾" and 1" horns. High gain horns attach
directly to the converter.

Processing Amplitude
Part # Type of Tip Volume Tip Diameter (microns)
4305 Replaceable Tip 50-500 ml 3/4" (19 mm) 120 μm

4306 Solid Tip 50-500 ml 3/4" (19 mm) 120 μm

4310 Solid Tip 100-1,000 ml 1" (25 mm) 60 μm

4311 Replaceable Tip 100-1,000 ml 1" (25 mm) 60 μm

9
 Q700 / Q500 Accessories High Throughput Horns

Part # Description
4 Tip Horn
4659 4 Tip Horn
The 4 Tip Horn enables 4 samples to be processed simultaneously. 4660 Replacement Tips
This horn offers high intensity and is effective for cell disruption,
mixing, homogenization and many other applications. Tip diameter
is 1/8" and the space between each tip is 1.05".

The 4 Tip Horn can process 1-15ml sample volumes

and is made to fit into both 1.5ml and 15ml tubes. When
processing small volumes with high intensity, samples
will heat up quickly. In addition to using the pulse mode,
a CoolRack tube cooling module is highly recommended.
CoolRack accessories work well with the 4 Tip Horn.

The 4 Tip Horn can be mounted in the #459 stand (as shown) or in the
Sound Enclosure (#432B2; see page 11).

24 Tip Horn Dual Horn

The 24 Tip Horn The Dual Horn allows a single Sonicator unit
processes each to process two samples simultaneously. The
well of a 24 well rectangular-shaped horn doubles the unit’s
plate simultaneously. output, and enables two probes to vibrate
This horn is effective with the same intensity as a single probe.
for cell disruption, The distance from center to center of each probe is 4.5".
mixing, dissolution and many ¾" solid tip probes are included with the Dual Horn but
other applications. ½" or 1" probes may also be used.

The 24 Tip Horn can be mounted The Dual Horn is capable of withstanding the rigors and harsh
inside the Sound Enclosure (page 11) chemicals of environmental testing labs. Sonication is used by
to reduce the noise level generated environmental labs to process soil and sediment samples in lieu
by sonication. Alternatively, a Heavy of soxhlet extraction methods. The Sonicator and Dual Horn
Duty Stand is available (page 17) meet the EPA requirements specified in method SW846-3550.
which allows precise adjustment of
the horn in and out of the microplate. Dual Horn components can be
ordered separately. The Dual Part # Description
Part # Description
Horn can be mounted in the 4525 Dual Horn with Probes
4579 24 Tip Horn
Sound Enclosure (#432B2) or Replacement ¾" (19 mm)
4208
4660 Replacement Tips on the Heavy Duty Stand (#438). Solid Probe

10
Q700 / Q500 Accessories Sound Enclosure

Sound Enclosure
Sonicators are extremely loud devices and
will cause discomfort to the user and anyone
nearby. The Sound Enclosure reduces noise
by approximately 20 dBa and is made to
work with all accessories (excluding the
Microplate Horn which has its own
dedicated enclosure).

In addition to reducing noise, the Sound

Enclosure has an internal support rod and
converter mounting system. Any Qsonica
probe or horn will be held safely and securely
inside the unit.

Two ports are located on either side

of the enclosure for coolant tubing or a
temperature monitoring probe. The interior
walls are lined with acoustical foam and the
door has a window so experiments can be
visually monitored.

432B2 shown with probe

(Probe sold separately.)

Part # Description
Sound Enclosure with
Converter Holder,
432B2 Exterior Dimensions (W x H x D)
13.5 x 30.5 x 13 in. 432B2 shown with cup horn
(343 x 775 x 330 mm) (Cup horn sold separately.)

11
 Q700 / Q500 Accessories Flocells

Low Volume Flocells

The Low Volume Flocell (LVF) is available for use with

either the Q500 or Q700 system. The Flocell (#4650)
is equipped with ¼" (6 mm) hose barb fittings and
does not include a probe. A ½" (12 mm) replaceable
tip probe (#4643) or ½" (13 mm) solid probe (#4644)
must be ordered separately. These probes feature
a flange for proper mounting with the LVF. The
replaceable tip probe is for use with aqueous samples
only. Solid tip probes can be used with all types of
LVF Assembly
solvents or low surface tension liquids. with Probe

Sonication generates heat so a Water Jacket (#4655)

is available if the process requires cooling. The water
jacket slides over the LVF and is used to recirculate
cold water around the exterior of the flocell body. The
water jacket includes ¼" (6 mm) hose barb fittings.

The LVF is recommended for processing sample

volumes above 1L. Routine applications include
cell lysis, mixing, solubilizing and deagglomerating/
dispersing nanoparticles.

Flocell Assembly Probe

with Water Jacket

Part # Description
4650 Low Volume Flocell
• Material: 316L Stainless Steel
• Operating pressure: 40-100 psi max.
• Internal volume: 65ml
• Maximum flow rate: 0.5L/min
• Hose barb fittings for ¼" (6 mm)
ID tubing
• Dimensions (H x D): 9.1 x 3.5 in.
228 x 89 mm) — dimensions
extra port include probe and clamp
outlet ½" (13 mm) Replaceable
4643
tip probe with flange for #4650
½" (13 mm) Solid tip probe
4644
with flange for #4650
inlet
4655 Water Jacket for #4650

12
 Q700 / Q1375 Accessories Flocells

How Flocells Work

Flocells offer inline or continuous, large volume, batch sample processing. Flocells are ideal
for mixing and dispersing applications. Batch volumes can be re-circulated through the system
multiple times if increased sonication time is needed. Multiple units can be used in series to
reduce processing time and/or maintain an even higher flow rate.

The liquid sample is pumped into the Flocell through the inlet at the bottom of the unit. As the
sample passes through the cavitation field, it is processed. The processed liquid exits the unit
through an outlet port. The degree of processing can be controlled by adjusting the intensity
of sonication as well as flow rate.

High Volume Flocells

The High Volume Flocell (HVF) is available for use

with either the Q700 or Q1375 System. The Flocell
(#4549) is equipped with ½" (13 mm) sanitary
connections, a water jacket and 1" (25 mm) HVF
Diameter probe (#4625). The water jacket can be Assembly

used to recirculate cold water around the exterior

of the flocell body. This helps reduce the heat
generated during ultrasonic processing.

Probe
The HVF is recommended for processing batch
volumes of 5L or more. Routine applications include
cell lysis, mixing, solubilizing and deagglomerating/
dispersing nanoparticles.

Part # Description
product 4549 High Volume Flocell
outlet • Dimensions (H x D): 17 x 16 in.
coolant (431 x 406 mm)
inlet
• Includes #4625 Probe
• Material: 316L Stainless Steel
coolant • Operating pressure: up to 100psi
outlet • ½" (13 mm) Sanitary connections
• Internal volume: 400ml
• Maximum flow rate: 20L/min
• Water jacketed for cooling
product inlet
4625 1"(25 mm) Diameter Flocell Probe

13
 Q700 / Q500 Accessories Indirect Horn Options

Cup Horn
A Cup Horn offers indirect sonication and functions as a high intensity
ultrasonic water bath. Multiple samples can be processed in sealed
tubes eliminating cross contamination or aerosol issues.

The horn is mounted within an acrylic cup and the cup is filled with
water. Sample tubes are placed in a rack at a fixed distance above
the ultrasonic horn. Cavitation is produced in the water, processing
the samples within the tubes. The #440 tube rack is included with
the Cup Horn. This rack is made for 1.5ml polystyrene tubes
which are proven to process samples more efficiently than 1.5ml
polypropylene tubes.
Part # 431C2
Inner Diameter of Cup: 5.5"
Sonication generates heat so ports for cooling are located on each
side of the cup. The #4900 Chiller is recommended for maintaining Diameter of Horn: 2.5"
both the water temperature and water level within the Cup Horn. Tube Rack #440
(8 tubes) is Included
Maintaining a fixed water level is extremely important and only the
Qsonica chiller can accurately control this variable.
Optional Tube Racks
The Sound Enclosure is highly recommended for all Cup Horn users.
In addition to reducing sonication noise to safe levels, it securely holds
the Cup Horn in place. The Sound Enclosure features ports on either
side to allow coolant tubing to pass from the Cup Horn to the Chiller.
Part #440 Part #451
Note: Selecting the appropriate size and type of sample tube will
greatly improve results. Contact Qsonica for application assistance.

Part #445 Part #449

Part # Description
Tube Holder 8 Tube Holder
# 440 (1.5ml Polystyrene tubes)
Tube Holder 8 Tube Holder
# 451 (1.5ml Polypropylene tubes)
Tube Holder 12 Tube Holder
# 449 (600ul PCR tubes)
Tube Holder 24 Tube Holder
# 445 (300ul PCR tubes)

14
 Q700 Accessories Indirect Horn Options

Microplate Horn
(Only for use with Q700)

Similar to a Cup Horn,

#Q700MPX with Chiller
but larger, the Microplate and tubing set
Horn is an indirect sonication
device capable of processing
an entire 96 well microtiter
plate or many microtubes
at one time.

Simply place your samples

within the water-filled
reservoir and the sonic
energy is transferred into
each individual well or tube.

The Horn is equipped with a clear acrylic collar to contain the liquid media within the reservoir. This
allows the user to process deep well microplates or other tall vessels. Standard microtiter plates or
PCR tubes require a smaller volume of liquid for sonication. For these applications, the clear acrylic
collar may be removed and the lower, gray collar will allow for easier access to the samples.

Part # Description
Q700MPX Q700 (Without Standard Probe), and the 431MPX
Microplate Horn, Pinch Clamps, Tubing
431MPX
and Sound Enclosure
431MPXH Microplate Horn Only

432MP Sound Enclosure for Microplate Horn

444 300 μl Microcentrifuge Tube Holder/Cover

Exterior dimensions of the Sound Enclosure are (W x D x H):
10 x 10 x 17 in. (254 x 245 x 432 mm).
#431MPX #431MPXH

The Microplate Horn is commonly used in PMCA research.

A microcentrifuge tube holder and cover (#444) are available
and often used for this application.

#444

15
 Q700 / Q500 Accessories Chillers

Recirculating Chillers
Sonication generates heat which may
be detrimental to some applications.
Attempting to control temperature with ice
and/or repeatedly changing out water is
tedious and no longer necessary. Qsonica
now offers 2 chiller options for automating
the sample cooling process.

Quick-connect tubing and fittings (ordered

separately) attach the chiller to the ports on
the cup horn or microplate horn. When used
in conjunction with the pulsed sonication #4900 #4905
mode, your desired water temperature will
be maintained. Older model cup horns may
require special fittings so please contact us
for ordering assistance.

Part # Description
4900 Compact Recirculating Chiller
Tubing and Connector Set
4910
for Cup Horn for #4900
Tubing and Connector Set
4915
for Microplate Horn for #4900
4905 High-Capacity Recirculating Chiller
Tubing/Connector Set for
4911
Cup Horn for #4905
Tubing/Connector Set
4916
for Microplate Horn for #4905 Chiller shown with Sonicator, tubing set, cup horn and sound enclosure (sold separately).

TECHNICAL SPECIFICATIONS: Part No. 4900 Part No. 4905

Cooling Capacity: 200 watts 400 watts
Temperature Range: 2-45°C 2-45°C
Dimensions: 7.5" x 5" x 7" (19 x 13 x 18 cm) 13" x 11" x 13" (32 x 28 x 32 cm)
Weight: 8 lbs. (3.6 kg.) 28 lbs. (12.7 kg.)
Voltage: 115-230 VAC, 50/60 Hz 115-230 VAC, 50/60 Hz

16
 Q700 / Q500 General Accessories

Replacement Converter Heavy Duty Stand

Part # Description Part # Description

CL334 Replacement Converter Heavy Duty

438
Stand
Converter & horn not included
Replacement Wrenches
Part # Description Precisely raises
404 Wrench Set and lowers the
converter and
probe/horn into
a sample vessel
Part # Description
Heavy Duty
4015
Pin Spanner

Temperature Monitoring Options

Part # Description
(Q700 only)
Temperature
4102
Probe
Part # Description Large Clamp Stand
Flexible
4103 Part # Description
Thermocouple
Stand with
2 types of ½" Diameter
temperature 459 Support Rod
probes are available and Converter
for use with the Clamp
Q700 Sonicator

Replacement Footswitch
Converter Cable

Jack Stand

Enables
Raises and lowers
hands free
sample vessels to
operation Part # Description a stationary probe
For Use as needed.
Part # Description FS-3
with Q700
K4 6 ft. Long Part # Description
For Use
K4-10 10 ft. Long 4004 357 Jack Stand
with Q500

17
 Q125 Sonicator
The Q125 is a microprocessor
based, programmable ultrasonic
processor. Features include pulse
mode and a digital display of both
wattage and joules.

The unit is effective for standard

cell disruption, DNA/RNA shearing,
homogenization and many other
applications. The Q125 is ideal for
small samples and for customers that Stand sold separately.
do not plan to scale up to larger volumes FEATURES:
in the future. This model offers the same Programmable operation
programming and display features as Set time and amplitude for hands free operation
the Q500 unit.
Pulse mode
Prevent heat buildup in temperature sensitive samples
Digital amplitude / intensity control
Output intensity can be set from 20-100%
ULTRASONIC Elapsed time indicator
START/STOP
Displays duration of sonication
PROCESSING POWER Display of wattage and joules
TIMER ON/OFF
Real-time energy monitoring

AMPLITUDE
Overload protection
PULSE/REST
ADJUSTMENT Prevents damage to circuitry if a fault occurs
AMPLITUDE RoHS compliant
Uses lead free components
Compact design
Takes up less space than competitive units

PART NO. Q125 INCLUDES: TECHNICAL SPECIFICATIONS:

• Generator Power Rating: 125 watts
• Converter Frequency: 20 kHz
• 1/8" (3 mm) diameter probe Programmable Timer: 10 hours
• Power cable Adjustable Pulse On/Off: 1 second to 1 minute
• Converter cable Dimensions (W x L x H): 8 x 13.75 x 5.75 in. (203 x 349 x 146 mm)
• Wrench set Voltage: 110V, 50/60 Hz
Specify desired voltage for export.

18
 Q125 General Accessories

Probes Sound Enclosure

Probes Part # 4423 Part # 4422 Part #4435

Processing
200 μl - 5 ml 500 μl - 15 ml 10 ml - 50 ml Part # Description
Volume*
Tip Diameter 5/64" (2 mm) 1/8" (3 mm) 1/4" (6 mm) Sound Enclosure with Converter Holder,
432A Exterior Dimensions (H x W x D)
Amplitude (μm) 200 180 120 20 x 12 x 12 in. (508 x 254 x 254 mm)

Support Stand Converter Cup Horn

Part # Description Part # Description
Support Replacement
CL18
Stand Converter
460 with
Converter
Holder

8 Tip Horn
Part # 4602 Part # 4608
Tip Diameter Horn Diameter
1/8" (3 mm) 25/32″(20 mm)
Distance Cup ID*
Between Tips 1.5" (38 mm)
0.35" (9 mm)
center to center *Accommodates (two)
1.5 ml microtubes or
(one) 15 ml Falcon tube.

19
Q55 Sonicator
The Q55 is a compact and cost effective
ultrasonic processor that will occupy less
bench space than any unit on the market.

This model is effective for standard cell disruption

and many other small volume applications.
Probes are available in three different sizes.

FEATURES:
• Smallest unit available
• Thumb-switch or continuous operation
• Simple and effective operation

CONTINUOUS
POWER PROCESSING
ON/OFF OR Stand sold separately.
THUMB-SWITCH
ACTIVATED
PROCESSING
PART NO. Q55 INCLUDES:
AMPLITUDE • Generator • Power cable
CONTROL
• Converter • Converter cable
• 1/8" (3 mm) • Wrench set
Probes Part # 4423 Part # 4422 Part # 4435 dia. probe

Processing
200 μl - 5 ml 500 μl - 15 ml 10 ml - 50 ml
Volume* TECHNICAL SPECIFICATIONS:
Tip Diameter 5/64" (2 mm) 1/8" (3 mm) 1/4" (6 mm) Power Rating: 55 watts

Amplitude (μm) 200 180 120 Frequency: 20 kHz

Dimensions 8 x 7.5 x 5.75 in.
(W x L x H): (203 x 190 x 146 mm)
Voltage: 110V, 50/60 Hz
Specify desired voltage for export.

Optional Accessories
Part # Description
Support Stand
460
with Converter Holder
Sound Enclosure with
Converter Holder
432A Exterior Dimensions (H x W x D)
20 x 12 x 12 in.
(508 x 254 x 254 mm)

CL188 Replacement Converter

#CL188 #460 #432A

20
CoolRacks
Tube Chilling Modules - Secure sample tubes
and standardize temperature during sonication.

CoolRack® thermo-conductive tube modules eliminate

inconsistencies which occur due to inserting tubes directly
into ice, dry ice, alcohol baths, water baths and other
common temperature sources. Place the module directly
onto the temperature source and it will rapidly adapt to that
temperature (from -196°C to +100°C). CoolRack modules
ensure temperature standardization of all tubes when cooling,
(snap) freezing or heating/thawing samples (within +/- 0.1°C)
and reproducible temperature-sensitive procedures.

PROBLEM SOLUTION
• Shifting Tubes • Fixed Tube Position
• Overheating Samples • Stable Temperature
• Inconsistent Tip Depth • Reproducible Results

1.5 ml Tubes 15 ml Tubes 50 ml Tubes

FEATURES: Part # Description For Use With

• Keep tubes at 501 6 Tube Rack 1.5 and 2 ml Tubes
desired temperature
502 15 Tube Rack 1.5 and 2 ml Tubes
• Prevent contamination
from ice/water 504 4 Tube Rack 1.5 and 2 ml Tubes
• Organize and keep
510 9 Tube Rack 15 ml Centriuge Tubes
tubes upright
• Resistant to rust 511 4 Tube Rack 50ml Centriuge Tubes
and corrosion 503 1 L Square Ice Pan #501, 502 or 504 CoolRacks
• Compatible with
detergents and 512 4 L Rectangular Ice Pan #510 or 511 CoolRacks
sterilizable #504 CoolRack 513 2.5 L Round Ice Pan #510 or 511 CoolRacks
(shown with 4 tip horn)

21
Q1375 Sonicator
The Q1375 offers the ability to process
industrial scale sample volumes in
individual batches or flow through
applications. This model includes a
1" diameter, 10" long probe and booster.
The booster increases the amplitude of
the probe which enables larger volumes
to be processed.

The Q1375 Sonicator offers the ability to

program processing times and a full range of
intensity settings. Processing time can be set
from 1 second to 10 hours. A pulsing feature is FEATURES:
also included. Pulsing can reduce the amount of Programmable operation
heat generated by sonication when processing Set time and amplitude for hands free operation
temperature sensitive samples. Pulse mode
Prevent heat buildup in temperature sensitive samples
Digital amplitude / intensity control
Output intensity can be set from 20-100%
PROCESSING ULTRASONIC Elapsed time indicator
TIMER START/STOP
Displays duration of sonication
POWER
ON/OFF Display of wattage and joules
Real-time energy monitoring
AMPLITUDE
ADJUSTMENT Overload protection
Prevents damage to circuitry if a fault occurs
AMPLITUDE
PULSE/REST Temperature protection
Prevents overheating of samples

PART NO. Q1375 INCLUDES:

• Generator TECHNICAL SPECIFICATIONS:
• Converter Power Rating: 1,375 watts
• 3:1 Booster Frequency: 20 kHz
• Converter cable Programmable Timer: 10 hours
• Power cable Adjustable Pulse On/Off: 1 second to 1 minute
• Wrench set Dimensions (H x W x D): 7 x 15 x 18.25 in. (178 x 380 x 463 mm)
• #4597 probe Voltage: 220V, 50/60 Hz

22
 Q1375 General Accessories

High Volume Flocells offer inline processing of larger sample volumes. Flocells are
In-Line Processing ideal for mixing, dispersing, lysing cells and many other applications.
The Q1375F package features a high power Sonicator with a High
Volume Flocell (HVF) for large scale processing.

The HVF has a maximum flow rate of 20 liters per minute. Individual
flow rates will vary depending on the type of sample, degree of
processing required and viscosity.
PART NO. Q1375F INCLUDES:
• Q1375 (without Standard Probe)
• 4549 HVF (see page 13)
• 4625 Flocell Probe

High Volume Full wave probes are required to reach down inside and process
Batch Processing samples within large vessels. Part #4597 is a standard probe
and works well for many applications. Larger volumes and more
difficult applications will benefit from the additional surface area
and higher power of the #4617 probe. #4662 features a 2" tip
diameter and can process an even larger volume than other
probe options. Contact us for assistance with selecting a probe
for your application.

Probes Part # 4597 Part # 4617 Part # 4662

Processing Volume* 500 ml - 10 L 2 L - 20 L 10 L - 30 L
Tip Diameter 1" (25 mm) 1" (25 mm) 2" (51 mm)
#4597 #4617 #4662 *Actual processing volumes may vary based on the application and type of sample.

Optional Accessories

Part # Description
Sound Enclosure with
Converter Holder (H x W x D)
4474
36 x 16 x 16 in.
(914 x 406 x 406 mm)
4060 Temperature Probe
Support Stand
461
and Converter Clamp
#4474 #4060 #461 *Stand and clamp can be used inside of enclosure.

23
 Frequently Asked Questions

Relationship between Sample Volume and Probe Size Processing

Tip Diameter Volume Range
Selecting the proper size probe is extremely important. Each probe 1/16" (2mm) 200ul - 2ml
has a recommended sample volume range. 1/8" (3mm) 1ml - 15ml
1/4" (6mm) 5ml - 50ml
Small volumes require a small tip to fit inside the sample tube.
Small tips (microtips) are recommended for processing samples 1/2" (12mm) 20ml - 250ml
inside small, thin vessels and never samples larger than 50ml. 3/4" (19mm) 100ml - 500ml
1" (25mm) 200ml - 1,000ml
Larger volumes require a larger probe for effective processing. For
1" with booster 500ml - 1,500ml
example, a 1" probe will process 1 liter more quickly than a ¾" probe.
Flocell Continuous flow

Replaceable vs. Solid Tips

Replaceable tip probes are used with aqueous samples. Replaceable

tip probes have threaded ends and when the tip is worn out it can be
unscrewed and replaced.

If you are processing a sample containing solvents or low surface

tension liquids you must use a solid tip probe. Solid tip probes can
be used for any type of sample. Replacable Solid

Tip Depth
Probes/tips must be submerged properly. If the tip is not submerged
enough the sample will foam or bubble. If the tip is too deep it will
not circulate the sample effectively.

Figure C indicates the correct set up and will achieve good results
in the shortest processing time. A B C

For more detailed information on the above topics, as well as the topics below,
visit the Literature section of [Link].

• Nanomaterials and Probe Size • Controlling Temperature • Power vs. Intensity

• Vessel Shape and Size • Cooling the Converter • How to Determine Energy Delivered
• Amplitude and Time Settings • Booster Horn

In addition, you will also find example protocols, publications, product manuals and warranty
information on the site.

24
[Link]

53 Church Hill Road

Newtown, CT 06470
phone 877.338.9636
fax 203.426.7026
email info@[Link]