Session 2019-20
CHEMISTRY
INVESTIGATORY PROJECT ON THE
AMOUNT OF CAFFEINE IN DIFFERENT
TEA SAMPLES
Submitted To :-
DIVYA SHARMA
Submitted by :-
MAYANK MEENA
Class 12th (PCM)
Roll No.
CERTIFICATION
This is to certify that MAYANK MEENA
student of class XII-A ,has completed his
research successfully on the project title
“Determination of caffeine in different
tea samples” under the guidance of Mrs.
DIVYA SHARMA (Subject Techer)
during the year 2019-20 in partial
fulfilment of chemistry practical
examination conducted by AISSCE, New
Delhi.
………………………….
Signature of teacher
……………………….
Signature of external
………………………….
Signature of Principal
DATE:-…………………
ACKNOWLEGMENT
I would like this opportunity to thank my
teacher, Mrs. Divya sharma, whose
support ,guidance and encouragement
have enabled me to complete this project.
I would also like to thank to my frnds also
for their corporation.
INDEX
1. Aim
2. Introduction
3. Theory
4. Apparatus & procedure
5. Observations
6. Conclusion
7. Evidence
8. Bibliography
AIM
“TO DETERMINE
THE AMOUNT OF
CAFEINE IN
DIFFERENT TEA
SAMPLES”
INTRODUCTION
THEORY
The most important methylated
alkaloid that occurs naturally is
caffeine. Its molecular formula is
CsH10N402. Its IUPAC name is 1,3,7-
trimethlxanthene and common
name is 1-methlated theobromine.
Purely it is white, crystalline solid
in the form of needles. Its melting
point is 123.c. it is the main active
principle component of tea
leaves.it is present in tea leaves
upto 3% and can be extracted by
first boiling the leaves with water
which dissolves many glycoside
compounds in the form of lead
complex. The clear filtrate is then
extracted with extracts caffeine
because it is more soluble in it then
water.
Effects of caffeine:
Positive effects
Acetylcholine is associated with
attention, concentration,
leaming, and memory but there
is no conclusive evidence yet
that caffeine has any effect on
memory and cognitive function.
Low doses of caffeine show
increased alertness and
decreased fatigue.
Caffeine has been shown to
increase the metabolic rate.
Negative effects
Caffeine can increase the blood
pressure in non-habitual
consumers.
Caffeine may reduce control of
fine motor movements (e.g.
producing shaky hands)
Caffeine can increase cortisol
secretion, some tolerance is
developed.
Caffeine withdrawal can
produce headache, fatigue and
decreased alertness.
High caffeine consumption
accelerates bone loss at the
spine in elderly postmenopausal
women.
APPARATUS
Green label tea, Yellow label tea,
red label tea, lead acetate,
chloroform, beakers, glass rod,
filter paper, funnel, water
PROCEDURE
1. First of all, 50 grams of tea
leaves were taken as sample
and 150 ml of water was added
to it in a beaker.
2. Then the beaker was heated
up to extreme boiling
3. The solution was filtered and
lead acetate was added to
filtrater, leading to the
formation of a curdy brown
colored precipitate.
4. We kept on adding lead
acetate till no more precipitate
had been formed.
5. Again solution was filtered.
6. Now the filtrate so obtained
was heated until it had become
50 ml.
7. Then the solution left was
allowed to cool.
8. After that,20 ml of
chloroform was added to it.
9. Soon after, two layers
appeared in the separating
funnel.
10. We separated the lower
layer.
11. The solution then exposed to
atmosphere in order to allow
chloroform to get evaporated.
12. The residue left behind was
caffeine.
13. Then we weighed it and
recorded the observations.
14. Similar procedure was
performed with different
samples of tea leaves and
quantity of caffeine was
observed in them.
OBSERVATIONS
1. Red Label Tea (Brooke Bond)
Weight of china dish –
Weight of china dish with precipitate –
Amount of caffeine --
2. Yellow Label Tea (Lipton)
Weight of china dish –
Weight of china dish with precipitate –
Amount of caffeine --
3. Green Label Tea (Lipton)
Weight of china dish –
Weight of china dish with precipitate –
Amount of caffeine-
CONCLUSION
Order of quantities of caffeine in different
samples of tea leaves:
Red label tea > Yellow label tea > Green
label tea
EVIDENCE
BIBLIOGRAPHY
Chemistry Laboratory manual
