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Hematology Microscope

The document describes the basic parts and functions of a compound light microscope. It lists the major components, including the mechanical stage, objectives, condenser, iris diaphragm, and light source. It explains that objectives provide different levels of magnification from low to high power. Objectives require specific working distances and numerical apertures to maximize resolution. The document also provides brief instructions for use and maintenance of the microscope.

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Mariel Abatayo
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161 views1 page

Hematology Microscope

The document describes the basic parts and functions of a compound light microscope. It lists the major components, including the mechanical stage, objectives, condenser, iris diaphragm, and light source. It explains that objectives provide different levels of magnification from low to high power. Objectives require specific working distances and numerical apertures to maximize resolution. The document also provides brief instructions for use and maintenance of the microscope.

Uploaded by

Mariel Abatayo
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

HEMALAB o Mechanical Stage – moves along x & y –axis

EX # 1 MICROSCOPE o Stage clips


o Mechanical stage adjustment knob
 Very impt optical instrument used to magnify minute organism/ specimen in o Condenser – gather beam of light and directs it to the specimen
question such that the details of its structure will be visible to the naked eye o Iris diaphragm – regulates amount of light w/c illuminates the objective
o Iris diaphragm aperture – moves left or right
 3 types: o Light source – gives correct brightness
1) SIMPLE – magnifying lens; single lens - most common: tungsten halogen light
2) COMPOUND – has 2 lenses (eyepiece/ocular and objectives); o Coarse adjustment knob – gives the general view/ outline of the field
- magnifies up to 1000x o Fine adjustment knob – gives detailed view of the structure of the specimen
- “ Brightfield Microscopy” o Light adjustment knob – controls the brightness of the light
3) ELECTRON – magnifies up to 1,000,000x o Switch

 Parts:  Cover w/ plastic when not in use to avoid accumulation of dust


o Eyepiece/ Oculars – 10x magnification (diameter of object being magnified)  Carry microscope w/ both hands; dominant hand on neck, the other on base
* x means diameter (if 10x, means object is magnified 10x)  Solvents used to clean the objectives of the microscope
- could either be Monocular or Binocular o Xylol alcohol/ Xylene - carcinogenic due to benzene
o Interpupillary control – determines distance between two oculars o Lense cleaner solution
o Body tube/ Optical tube – holds the eyepiece in place o 70% isopropanol
o Arm/ Neck – provides support; attachment site of revolving nosepiece
o Revolving nosepiece – holds objectives in place; allows easy rotation of
objectives
o Objectives
* Low Power Objective
- working distance = 16 mm
- magnification power = 10x; total magnification = 100x
* High Power Objective/ High-dry objective/ high & dry objective
- working distance = 4 mm
- magnification power = 40x; total magnification = 400x
 numerical aperture (ability of microscope to gather light) = 0.65
> resolution (ability of mic to focus finer details of specimen)
> increased resolution = increased quality
 optical tube length (distance bet ocular & objective) = 160 mm
* Oil Immersion Objective
- working distance = 1.8 mm
- magnification power = 100x; total magnification = 1000x
 Oil (classified acc to viscosity)
> type A – low viscosity (fluorescence & darkfield mic.)
> type B – high viscosity (brightfield & clinical mic.) = Cedar oil
> type C– very high viscosity (inclined mic./ long objectives)

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