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Lab Notes: Lipids: Tests Test For Reagents Principles Positive Result

The document lists several common lipid tests, including the reagents used, principles, and positive results. The bromine water test detects unsaturation of lipids by decolorizing iodine solution. The acrolein test identifies fats or glycerol by producing a burnt fat odor. The Molisch test detects all carbohydrates using alpha-naphthol to form a purple ring.

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Betina De Jesus
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164 views1 page

Lab Notes: Lipids: Tests Test For Reagents Principles Positive Result

The document lists several common lipid tests, including the reagents used, principles, and positive results. The bromine water test detects unsaturation of lipids by decolorizing iodine solution. The acrolein test identifies fats or glycerol by producing a burnt fat odor. The Molisch test detects all carbohydrates using alpha-naphthol to form a purple ring.

Uploaded by

Betina De Jesus
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

LAB NOTES : LIPIDS

TESTS Test for Reagents Principles Positive


Result
Bromine unsaturation of methylene chloride hydrogenation or decolorization of
water test lipid (CH2Cl2), iodine halogenation via the iodine solution
solution addition (orange to yellow
solution)
Acrolein test fats or glycerine glycerol, dehydration burnt fat odor
(glycerol) potassium
bisulfate (KHSO4),
concentrated
sulphuric acid
(conc. H2SO4)
Molisch test all carbohydrates alpha-naphthol dehydration and purple ring
(molisch’s condensation
reagents),
conc. H2SO4
Ninhydrin alpha-amino (NH2) ninhydrin reagent decarboxylation, blue-violet solution
test or alpha-imino deamination and
(NH) group condensation

Soda lime test amino (NH2) or sodium hydroxide deamination red to blue litmus
imino (NH) group (NaOH), calcium paper
oxide (CaO)
Ammonium phosphate group nitric acid (HNO3) hydrolysis and yellow precipitate
molybdate double
test decomposition
Liebermann- cholesterol acetic anhydride dehydration and dark-green
burchard test sterol and sulfuric acid oxidation solution

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