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Biochem Experiment (Sakaguchi Test)

The Sakaguchi test detects the amino acid arginine. It involves adding alpha-naphthol and sodium hypochlorite to samples of 1% arginine, 1% glycine, and 5% egg white, which will form a red complex if arginine is present due to its guanidinium group. The test relies on the ability of arginine to condense with alpha-naphthol via its guanidinium group to identify the presence of arginine in a protein sample.

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2K views2 pages

Biochem Experiment (Sakaguchi Test)

The Sakaguchi test detects the amino acid arginine. It involves adding alpha-naphthol and sodium hypochlorite to samples of 1% arginine, 1% glycine, and 5% egg white, which will form a red complex if arginine is present due to its guanidinium group. The test relies on the ability of arginine to condense with alpha-naphthol via its guanidinium group to identify the presence of arginine in a protein sample.

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Camilogs
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
  • Sakaguchi Test Overview: Describes the objective of the Sakaguchi test, which is to detect guanidinium groups like those in arginine.
  • Research Questions and References: Provides follow-up questions for research and references for further reading about the Sakaguchi test.

SAKAGUCHI TEST

OBJECTIVES:

To detect amino acid guanidinium group like arginine.

MATERIALS:

APPARATUSES TO MATERIALS TO SAMPLES TO BE TESTED:


BE USED: BE BROUGHT:

 3 Test tubes N/A  1 mL of 1% arginine + 2 drops of a-naphthol +


 1 Graduated 2 mL sodium hypochlorite + 1 mL of urea
Cylinder solution
 1 mL of 1% glycine + 2 drops of a-naphthol + 2
mL sodium hypochlorite + 1 mL of urea
solution
 1 mL of 5% egg white (albumin) + 2 drops of
a-naphthol + 2 mL sodium hypochlorite + 1 mL
of urea solution

PROCEDURE:

1. Put 1mL of 1% arginine, 1 mL of 1% glycine, 1 mL of 5% egg white (albumin) in to


different dry test tubes.
2. Add 2 drops of α-naphthol to the three samples and mix well.
3. Now add 2 mL sodium hypochlorite to all test tubes.
4. Immediately add 1mL of urea solution to establish the red complex formed.

DATA:
QUESTIONS FOR RESEARCH:

1. What is the principle involved in the Sakaguchi Test?

2. How was the arginine able to condense with the alpha-naphthol (a-naphthol) to
give a red solution?

3. What is the significant of Sakaguchi Test?

4. What are the chemical characteristics of amino acids? How does this affect
protein structure?

5. What is guanidinium group?

REFERENCES:

http://www.onlinebiologynotes.com/sakaguchi-test-objective-principle-reagents-
procedure-and-result/

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