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Trans-Blot Turbo Transfer Protocol Guide

This document provides instructions for using Trans-Blot Turbo Transfer Packs to transfer proteins from a gel to a membrane using the Trans-Blot Turbo Transfer System. The instructions explain how to assemble the gel, membrane and buffer stacks in the cassette for single or double mini gels or a single midi gel. Pre-programmed protocols are listed for different protein sizes and gel configurations. Notes provide additional guidance on avoiding drying the membrane and inspecting the instrument after use.

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1K views2 pages

Trans-Blot Turbo Transfer Protocol Guide

This document provides instructions for using Trans-Blot Turbo Transfer Packs to transfer proteins from a gel to a membrane using the Trans-Blot Turbo Transfer System. The instructions explain how to assemble the gel, membrane and buffer stacks in the cassette for single or double mini gels or a single midi gel. Pre-programmed protocols are listed for different protein sizes and gel configurations. Notes provide additional guidance on avoiding drying the membrane and inspecting the instrument after use.

Uploaded by

bb1abdim
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

BLOTTING

Trans-Blot Turbo Transfer System


Transfer Pack
Quick Start Guide
Instructions for Using Trans-Blot Turbo Transfer Packs
Mini Transfer Packs Midi Transfer Packs
The anode stack and membrane are The anode stack and membrane are
located in the right Bottom (+) well. located on top; use the right Bottom (+)
finger tab to remove.

1. Place the membrane and bottom 4. Close and lock cassette lid. Insert the
stack on the cassette base.* cassette into the instrument and begin
■■
Use the blot roller to remove trapped air bubbles transfer.
■■
1 mini or midi gel: place stack in the center of
the cassette * For detailed instructions, please refer to
■■
2 mini gels on mini stacks: place stacks side by the Trans-Blot Turbo Blotting System
side in the center of the cassette Instruction Manual.
■■
2 mini gels on midi stack: place midi stack in the
Top (–)
center of the cassette cassette
electrode
2. Place gel on top of membrane. (cathode)
■■
Do not equilibrate the gel before transfer
■■
Remove any air bubbles with blot roller Top ion
reservoir stack
■■
2 mini gels on midi stack: place foot of gels
Gel
toward the center
Blotting
membrane
3. Place second wetted transfer stack on top
Bottom
of gel. This will serve as the top ion stack. ion reservoir
stack
■■
 oll the assembled sandwich with blot roller to
R
expel trapped air bubbles Bottom (+)
■■
 lease refrain from adding any extra transfer buffer
P cassette
electrode
to the cassette; saturated transfer stacks provide (anode)
ample transfer buffer

Please recycle transfer pack trays.


Acceptable* Not Acceptable*
Combination 1 2 1 2
Upper bay A 1 mini gel 2 mini gels 1 mini gel 2 mini gels
or 1 midi gel or 1 midi gel
Lower bay B 1 mini gel 2 mini gels 2 mini gels 1 mini gel
or 1 midi gel or 1 midi gel
* Conditions hold if trays are swapped.

Bio-Rad Preprogrammed Protocols


Protocol Name MW, kD Time, min 2 Mini Gels or 1 Midi Gel 1 Mini Gel
STANDARD SD Any 30 Up to 1.0 A; 25 V constant
1.5 MM GEL Any 10
HIGH MW >150 10 2.5 A constant; 1.3 A constant;
up to 25 V up to 25 V
LOW MW <30 5
MIXED MW* 5–150 7
1 Mini TGX* 5–150 3 N/A 2.5 A constant;
up to 25 V
* Also accessed via the TURBO navigation button.

Notes
■■
 els do not require equilibration and can be
G ■■
 ransfer stacks will be warm after transfer. Avoid
T
transferred immediately after electrophoresis drying the membrane during stack disassembly
■■
 o prevent the membrane from drying out,
T ■■
 lean and inspect the instrument and cassette after
C
use transfer packs immediately after opening each use for damage. Contact Bio-Rad technical
support if any damage is noted

Call 1-800-4BIO-RAD (1-800-424-6723) or visit bio-rad.com for technical support.

Bio-Rad and Trans-Blot are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions.
All trademarks used herein are the property of their respective owner.

10000100869 Ver F (10019593) 18-0608 0718

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