Lecture 11 and 12 – Autonomic nervous system

Material for lecture is from the following links

1)
http://www.ib.cnea.gov.ar/~redneu/2013/BOOKS/Principles%20of%20Neural%20Science%20-%20Kan
del/gateway.ut.ovid.com/gw2/ovidweb.cgisidnjhkoalgmeho00dbookimagebookdb_7c_2fc~57.htm
2) Links provided at the bottom of the slide

Raghav Rajan
Bio 354 – Neurobiology 2
February 11th 2015
BIO 354 - Neurobiology 2 1
Divisions of the nervous system

BIO 354 - Neurobiology 2 2

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What is the function of the autonomic nervous system?

●
“Fight or Flight”
●

●
Largely co-ordinates visceral and reflexive actions
●
Mostly not under conscious control (there are exceptions)
●
Senses the internal environment of the body and acts
accordingly
– Consists of both visceral sensory and motor neurons
●
Also called “involuntary nervous system”

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 Somatic
and
Autonomic
reflexes
●
Differ in the
involvement
of CNS
●
Differ in the
targets

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 Autonomic nervous system had 3 sub-divisions

●
Sympathetic - “Fight or Flight”
(Walter Cannon)
●
Parasympathetic - “Rest and
digest” (Walter Cannon)
●
“Homeostasis” - main function to
maintain constant internal
environment (negative feedback
regulation)
●
Hypothalamus is the “boss”
●
Enteric – related to the gut and
mediates digestive reflexes
– More independent than the other
two
– Very few connections to the rest
of the nervous system

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http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/bio%20102/bio%20102%20lectures/nervous%20system/nervous1.htm
 An example of autonomic
nervous system function
Regulation of water in the
body - thirst

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https://voer.edu.vn/c/water-balance/948ed3b1/04a5f05f
Differences between somatic and autonomic motor
systems : 1) location of motor neuron
●
Motor neurons of autonomic
nervous system are outside
CNS
– Preganglionic fiber from CNS
– Postganglionic fiber to
target
●
One extra synapse in the
path from CNS to peripheral
target – autonomous nervous
system

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 Differences between somatic and autonomic motor
systems : 2) synaptic terminals

●
Nerve endings that don't have pre-synaptic boutons
●
Rather, presence of multiple varicosities along the length – mutliple release sites

●
Small number of fibers providing diffuse innervation – longer range effect than
normal neurotransmitters

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 Organisation of
sympathetic nervous
system
●
Thoracolumbar system
●
Originates from thoracic and
upper lumbar spinal cord
●
Chain of ganglia next to the
spinal cord
– Exceptions – 3 ganglia that
control the gut
●
Pre-ganglionic fibers – short,
myelinated
●
Post-ganglionic fibers – long,
unmyelinated

https://voer.edu.vn/m/divisions-of-the-
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 Organisation of
projections to
sympathetic
system
●
3 major types
●
Depends on
origin and
target or
projection

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 Organisation of
parasympathetic nervous
system
●
Craniosacral system
●
Cranial and spinal nerves
– origins or preganglionic
fibers
●
Ganglia are close to
targets or within targets
●
Preganglionic fibers – long
●
Postganglionic fibers -
short

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BIO 354 - Neurobiology 2 autonomic-nervous-system/1121e1d0#tbl- 11
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Example of autonomic nervous system – pupil dilation
and contraction

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Circuit that controls changes in pupil size

●
Only one part
of the circuit

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 Separate sympathetic and
parasympathetic control

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https://voer.edu.vn/m/autonomic-reflexes-and-homeostasis/0058b664
 Sympathetic and
parasympathetic
nervous system –
work
antagonistically

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chive/2012/suggested_reading_list/sup
BIO 354 - Neurobiology 2 plements/figures/sympathetic_parasym
15

pathetic.jpg
How do you get different responses in the targets?

●
Otto Loewi – Nobel prize in 1934 – for the discovery of
chemical neurotransmission – ACh (Vagusstoff)
●
His experiments were done in 1921 – at a time when it
was not clear if neurotransmission was chemical or
electrical

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 Interesting history pertaining to his discovery

On mature consideration, in the cold light of the morning, I would not have done it.
After all, it was an unlikely enough assumption that the vagus should secrete an
inhibitory substance; it was still more unlikely that a chemical substance that was
supposed to be effective at very close range between nerve terminal and muscle be
secreted in such large amounts that it would spill over and, after being diluted by the
perfusion fluid, still be able to inhibit another heart. (Loewi 1921)
While it remains unclear how much of Loewi's account is true, Loewi was fortunate
in his choice of experimental preparation. In the species of frog used (Rana esculenta
), the vagus contains both inhibitory and stimulatory fibers. In the winter, inhibitory
fibers predominate, so Loewi was also fortunate to have performed his experiments
in February or March. Additionally,acetylcholinesterase activity (the enzyme that
degrades acetylcholine) is low, particularly in an unheated laboratory, allowing the
neurotransmitter to remain long enough to be collected and applied to a second heart.
Thanks to this confluence of events, Loewi was able to describe the existence
of vagusstoff and prove the existence of chemical synaptic transmission

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Increase and decrease in heart-rate mediated by two
different neurotransmitters
●
Two different
neurotransmitters
– NE (norepinephrine)
●
adrenergic
receptors – alpha
and beta
– ACh (Acetyl Choline)
● cholinergic
● muscarinic
receptors

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Neurotransmitters typically used by both systems

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Other neuropeptides also used

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 Enteric nervous system

●
Very large number of sensory, motor and interneurons
●
Controls smooth muscles of the gut, local blood vessels and secretions of
the mucosa
●
Two major networks of nerves and vessels
– Myenteric plexus – gut motility
– Submucous plexus – secretions of mucous
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Multiple time-scales of responses mediated by the
different transmitters and different receptors
●
Synapses in the
sympathetic chain
ganglia of bullfrog
●
Single pre-synaptic
stimulation in both
evokes fast EPSPs
●
Repetitive stimulation
evokes different
responses in both
●
Peptidergic EPSP
evoked in second
synapse only when
first one is stimulated

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Another example of the influence of neuropeptides

●
Different neuropeptides add to the
complexity

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Central autonomic networks - input

●
Cranial nerves VII, IX
and X convey
information to the
brainstem - NST
(nucleus of the solitary
tract)

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Central autonomic network - outputs
●
NST, and other nuclei in the
brainstem and medulla provide
direct outputs to sympathetic
ganglion
●
A number of other forebrain and
midbrain nuclei are also connected
to this system
●
Anterior insula – visceral sensory
cortex – gets information about
internal state
– Topographic map of internal organ
systems
– Lesions cause loss of appreciation of
visceral sensations
●
Infralimbic area – part of cingulate
cortex – visceral motor cortex
– Lesions – abulia – loss of emotional
responses to external stimuli
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 Hypothalamus

●
Integrates autonomic
responses and endocrine
function with behavior
– Controls blood pressure
and electrolyte
composition
– Regulates body
temperature
– Controls energy
metabolism
– Regulates reproduction
– Controls emergency
responses to stress
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Three main mechanisms used by the hypothalamus to
function
●
Gets all sensory input
– From all sense organs (direct or indirect)
● visual input used to send information to suprachiasmatic nucleus
to regulate circadian rhythm
– Has internal sensory neurons that respond to changes in
temperature, glucose, etc.
– Circulating hormones can also enter near the margin and
directly interact with hypothalamic neurons
●
Compares sensory information with biological set points
●
If there is a deviation from set point, it triggers various
behavioral, autonomic and endocrine responses to restore
homeostasis
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 Hypothalamus – many
divisions
● Anterior third – preoptic area
– integration of sensory
information needed to judge
deviation from set point
● Middle third – Dorsomedial,
ventromedial, paraventricular,
supraoptic, arcuate
– control of growth, feeding,
maturation, reprduction
● Posterior third – mamillary
body, posterior hypothalamus
– regulating wakefulness and
arousal
– other function not known

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Also controls endocrine system through the pituitary

●
Peptidergic neurons (5) release
hormones into circulation through
posterior pituitary
●
Anterior pituitary control – two
types of neurons
– Peptidergic neurons (3, 4) release
hormones into the hypophyseal-
portal circulation
– Monaminergic neurons (1,2) link
the rest of the brain with
peptidergic neurons – can contact
at axon terminals or cell body

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The paraventricular nucleus is a part of the complex
control of autonomous and endocrine functions
●
Magnocellular hormone
releasing neurons

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 Eg: regulation of body temperature

●
Pigs can be
trained to
switch on IR
heaters
●
Thermodes to
cool or heat
the
hypothalamus

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1365459/pdf/jphysiol01120-0154.pdf
 Both ambient
temperature
and
hypothalamus
temperature
influence
warmth
seeking
behavior

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Neurons in the hypothalamus respond to changes in
temperature
●
A large
number of
neurons are
temperature
insensitive (>
70%)
●
About 20% of
the neurons
change their
firing rate
with changes
in
temperature

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 Ionic basis of these responses

●
Preoptic neurons in Rat
hypothalamic tissue slices
●
Temperature insensitive
neurons
●
Warm sensitive neurons –
IA (K+ current) – rates of
inactivation are made
faster at warmer
temperatures
Fast after
hyperpolarisation

Slow depolarising http://jap.physiology.org/content/100/4/1347

pre-potential BIO 354 - Neurobiology 2 34
Model for thermoregulation and neuronal basis

●
Heat loss
effector
neurons
●
Heat
production
effector
neurons

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 Putative heat-effector neurons

●
Recording from EPSP driven neurons
●

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Putative heat production effector neurons

●
Cold-sensitive neurons
●
Synaptic blockade makes them
lose their cold-sensitivity

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Model for thermoregulation and neuronal basis

●
Heat loss
effector
neurons
●
Heat
production
effector
neurons

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http://jap.physiology.org/content/100/4/1347
Different neuronal morphologies in the preoptic
nucleus
●
The different dendritic
orientations fits with the
known anatomy of
peripheral inputs
●
Silent neurons can only be
driven by current injection
●
Receive IPSPs from warm-
sensitive neurons
●
May be an EPSP-driven
neuron – synaptic input
from afferents or remote
neural sites
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Important differences between earlier models

●
Temperature
insensitive neurons
do not respond to
peripheral
temperature
changes (thought to
respond to cold-
sensitive peripheral
inputs)
●
Warm-sensitive
neuron is potentially
a sensor as well as
an integrator
(thought to respond
to warm-sensitive
peripheral inputs)

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General model for regulation of different body
parameters with a set-point
●
Set-point is the
balance
between
synaptic
inhibition and
synaptic
excitation
●
Can be moved
around by
changing firing
rates of either
neuronal type
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What is the function of the autonomic nervous system?

●
“Fight or Flight”
●

●
Largely co-ordinates visceral and reflexive actions
●
Mostly not under conscious control (there are exceptions)
●
Senses the internal environment of the body and acts accordingly
– Consists of both visceral sensory and motor neurons
●
Also called “involuntary nervous system”
●
Pretty complex
●
Has a number of specializations that help co-ordinate a variety of
responses with small set of fibers
●
Important for homeostasis – maintenance of constant internal
environment of the body
BIO 354 - Neurobiology 2 42