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Lod

The document discusses the limit of detection (LOD) and limit of quantification (LOQ) for analytical procedures. The LOD is the lowest concentration of an analyte that can be detected but not quantified, while the LOQ is the lowest concentration that can be quantified with acceptable accuracy and precision. Both can be determined visually by establishing the minimum detectable/quantifiable level, or based on a signal-to-noise ratio of 3:1 for LOD and 10:1 for LOQ. They can also be calculated using the standard deviation of low concentration responses and the slope of the calibration line. LOD and LOQ are important parameters for determining impurities and degradation products at low levels in samples.

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297 views2 pages

Lod

The document discusses the limit of detection (LOD) and limit of quantification (LOQ) for analytical procedures. The LOD is the lowest concentration of an analyte that can be detected but not quantified, while the LOQ is the lowest concentration that can be quantified with acceptable accuracy and precision. Both can be determined visually by establishing the minimum detectable/quantifiable level, or based on a signal-to-noise ratio of 3:1 for LOD and 10:1 for LOQ. They can also be calculated using the standard deviation of low concentration responses and the slope of the calibration line. LOD and LOQ are important parameters for determining impurities and degradation products at low levels in samples.

Uploaded by

Bob Cutter
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

LOD (limit of detection)

The detection limit of an individual analytical procedure is the lowest amount of analyte in a sample
which can be detected but not necessarily quantitated as an exact value. Several approaches for
determining the detection limit are possible.

Based on visual evaluation: The detection limit is determined by the analysis of samples with known
concentrations of analyte and by establishing the minimum level at which the analyte can be reliably
detected.

Based on Signal-to-Noise Approach: Determination of the signal-to-noise ratio is performed by


comparing measured signals from samples with known low concentrations of analyte with those of
blank samples and by establishing the minimum concentration at which the analyte can be reliably
detected. A signal-to-noise ratio between 3 or 2:1 is generally considered acceptable for estimating
the detection limit.

Based on the Standard Deviation of the Response and the Slope: The detection limit (DL) may be
expressed as:=3X STANDRD DEVIATION OF LOW CONCEN/ SLOPE OF THE CALIBRATION LINE

LOQ (limit of quantification)

The quantification limit of an individual analytical procedure is the lowest amount of analyte in a
sample which can be quantitatively determined with suitable precision and accuracy. The
quantification limit is a parameter of quantitative assays for low levels of compounds in sample
matrices, and is used particularly for the determination of impurities and/or degradation products.

Based on visual evaluation: The detection limit is determined by the analysis of samples with known
concentrations of analyte and by establishing the minimum level at which the analyte can be
quantified with acceptable accuracy and precision.

Based on Signal-to-Noise Approach: Determination of the signal-to-noise ratio is performed by


comparing measured signals from samples with known low concentrations of analyte with those of
blank samples and by establishing the minimum concentration at which the analyte can be reliably
quantified. A typical signal-to-noise ratio is 10:1.

Based on the Standard Deviation of the Response and the Slope: The quantitation limit (QL) may be
expressed as: 10XSTANDRD DEVIATION OF LOW CONCEN/ SLOPE OF THE CALIBRATION LINE

LOD and LOQ are the smallest amount of the particular compound that can by
detected and quantiified by using the developed HPLC method.
The signal to noise ratio is that minimum amount which when injected in HPLC it
gives minimum detectable peak area.
The value of amount at this point is multiplied by 3 to get LOD
and by 10 to get LOQ value. In short LOD is the thrice value of minimum value and
LOQ is ten times of minimum value that can be injected in HPLC.

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