SPECTROPHOTOMETER
V-1600
V-1800
UV-1600
UV-1800
USER’S MANUAL
P/N: 435031 REV. 1.0.0
Contents
Safety ............................................................. 1
Package Contents ................................................... 1
Unpacking .......................................................... 2
Installation ....................................................... 2
1. Environment Required.................................................................................................. 2
2. Install Spectrophotometer ...................................................................................... 2
Overview ........................................................... 3
Symbols ............................................................ 3
Main Specifications ................................................ 3
Description of Appearance and Keys ................................. 4
1. Appearance ......................................................................................................................... 4
2. Keypad ................................................................................................................................... 5
3. Description of Keys .................................................................................................... 6
Functions .......................................................... 7
Getting Started .................................................... 7
Important Guidelines ............................................... 8
General Operating .................................................. 8
Measuring ......................................................... 10
1. Photometry ....................................................................................................................... 10
2. Quantitation................................................................................................................... 11
3. Kinetics ............................................................................................................................ 15
4. Utility............................................................................................................................... 17
Turn On/Off D2 Lamp .......................................................................................................... 17
Turn On/Off W Lamp ............................................................................................................. 17
Set Date & Time .................................................................................................................... 18
Get Dark Current ................................................................................................................. 18
Reset Wavelength ................................................................................................................. 18
Lamp Life .................................................................................................................................. 19
I
Load Default Parameters ................................................................................................. 19
Lamp Change ............................................................................................................................. 19
About Version......................................................................................................................... 20
Troubleshooting ................................................... 20
Repair and Maintenance ............................................ 21
1. Daily Maintain .............................................................................................................. 21
2. Spare Parts Replacement ........................................................................................ 21
Warranty .......................................................... 26
Equipment Disposal ................................................ 26
II
Safety
Please follow the guidelines below, and read this manual in its
entirety to ensure safe operation of the unit.
We recommends against the use of V-1600/V-1800/UV-1600/UV-1800
Spectrophotometer.
Do not open the device.
Disconnect the device from the mains supply before
carrying out maintenance work or changing the fuses.
The inside of the device is a high-voltage area Danger!
Do not use the device if it is damaged, especially if
the main power cable is in any way damaged or defective.
Repairs may only be carried out by the service
technicians from us and authorized contractual
partners.
The device must be connected to a power outlet that
has a protective ground connection.
If the equipment is used in a manner not specified by
the manufacturer, the protection provided by the
equipment may be impaired.
Do not allow any liquid to enter into the device.
Do not operate the device in a hazardous location or
potentially explosive environment.
Package Contents
Description Quantity
Spectrophotometer 1PC
10mm Glass Cuvette 4PCS
10mm Quartz Cuvette ① 2PCS
Power Cord 1PC
User’s Manual 1PC
Dust Cover 1PC
① Only for model UV-1600/UV-1800.
1
Unpacking
Open the package, according to carefully check the packaging packing
list items, if found inside the packaging are missing or damaged items
please contact us and authorized contractual partners.
Installation
1. Environment Required
To ensure the best performance, the following conditions are required:
The best working temperature range is 16—35℃ and the humidity
is 45—80%.
Keep it as far as possible away from the strong magnetic or
electrical fields or any electrical device that may generate
high-frequency fields.
Set the unit up in an area that is free of dust, corrosive gases
and strong vibrations.
Remove any obstructions or materials that could hinder the flow
of air under and around the instrument.
The power requirement is 110±11V/60±1Hz or 220±22V/50±1Hz.
Use the appropriate power cord and plug into a grounded outlet.
If the local voltage is not stable, a voltage regulator is
required.
Be away from direct sunlight.
2. Install Spectrophotometer
Placement
Place the instrument on the stable table carefully.
Install Printer (Printer is Optional Accessories)
Check to confirm instrument power switch is turned off, connect the
printer’s data cable to the instrument’s parallel port.
Link the Power Cord
Check to confirm instrument power switch is turned off, the power cord
plug into two separate power interface and power supply socket
apparatus.
2
Overview
V-1600/V-1800/UV-1600/UV-1800 Spectrophotometer is an electrical
measure instrument which is widely used in the laboratories.
Use Frequency: Intermittence
Excessive Voltage(Current): No
Pollution Class: Class 1
Symbols
The following chart is an illustrated glossary of the symbols that are
used in this manual.
Caution, Danger!
Caution, High Voltage!
Caution, Hot!
Ground
Fuse
Recycle, this instrument will be called back by the
appointed Electrical Treatment Department or by the
original Manufacturer when wasted.
Main Specifications
Optical System Single beam
Wavelength Range 190—1100nm (UV-1600/UV-1800),
320—1100nm (V-1600/V-1800)
3
Wavelength Accuracy ±0.5nm
Wavelength Repeatability 0.3nm
Photometric Range -0.3—3A, 0—200%T
Photometric Accuracy ±0.5%T
Photometric Repeatability 0.3%T
Spectral Bandwidth 4nm (UV-1600/V-1600),
2nm (UV-1800/V-1800)
Stray Light 0.05%T@220nm&360nm(UV-1600
/UV-1800),
0.05%T@360nm (V-1600/V-1800)
Stability ±0.002A/h@500nm
Work Mode Photometry, Quantitation, Kinetics
Interface USB, Parallel(printer)
Power Requirement AC 110/220V, 50/60Hz
Dimensions 490x360x240
Weight 12kg(V-1600/V-1800),
14kg(UV-1600/UV-1800)
Work Environment 15—35℃, 15—70% relative humidity
Store Environment -10 — 50 ℃ , 15 — 70% relative
humidity
Description of Appearance and Keys
1. Appearance
Front View
1
3
Back View
4
8 11
6
5 7 9 10
1 LCD Display 7 USB port
2 Keypad 8 Cover of Fan
3 Lid of Sample Room 9 Power Socket
4 Rod 10 Power Switch
5 LCD Contrast Adjust 11 Cover of Cooling Vents
6 Printer port
2. Keypad
5
3. Description of Keys
S ET SET Key: Set Parameters
GOTO ʎ GOTO ʎ Key: Set Wavelength
ZERO ZERO Key: Blank
PRIN T PRINT Key: Print measuring result
6
0 9
… ,
Numeric Keys: Enter numbers
CLEAR Key: Delete the input value or stored
CLEAR
data
RETURN RETURN Key: Return to previous interface
S TART
START/STOP Key: Start/Stop testing
S TOP
EN TER ENTER Key: Confirm operation
UP, DOWN Keys: Scroll menu/data and set Y scale
,
Functions
Photometry
Display results as Abs, %T or Energy.
Quantitation
We provide 2 methods to establish a Standard Curve:
Up to 9 Standard Samples to establish Standard Curve;
Input coefficients to establish Standard Curve.
Kinetics
Record up to 1000 points;
2 Photometric Mode to display the curve (%T-Time & Abs-Time).
Getting Started
The following chart describes the basic operation of the instrument.
Turn On and Self-check
7
Switch on the power. Then the
instrument begins to self-check √Sel f - t est i ng
and 20 minutes’ warm up. Self-
Fi l t er
check includes the following
steps: Turn on lamps → Check W Lamp
Sensor → Initialize AD → System D2 Lamp
position → Get Dark Current → Warm
up.
War m up 20 mi nut es,
“ENTER” t o ski p
After warm up, instrument displays
Main Interface. Phot omet r y
Quant i t at i on
Ki net i cs 01/ 01
Ut i l i t y 00: 00
Important Guidelines
Reagents and dilution buffers can cause cauterization and other
damage to health.
Samples (nucleic acids, proteins, bacteria cultures) can be
infectious and cause serious damage to health.
During sample preparation, measuring procedures and maintenance and
cleaning work, observe all local laboratory safety precautions (e.g.
wear protective clothing and gloves, use of disinfectant) regarding
the handling of sample material.
Dispose of measuring solutions and cleaning and disinfectant
materials in accordance with the relevant local laboratory
regulations.
General Operating
Select Application
8
Main Interface, press numeric key or , to choose corresponding
EN TER
menu, then press .
Set Wavelength
GOTO ʎ
Press to set wavelength, input the values by pressing Numeric
EN TER
keys, press to go to the point you set, then do blank
automatically.
Phot omet r y
WL=546. 0nm
Pl ease ent er WL
WL=
Set Parameters
S ET
In different application, press to set parameters, press ,
EN TER
to choose or input the values by numeric keys, press to
RETURN
enter into, press to return.
Set Auto-cell Holder (optional)
Press the numeric key (1-8) to make corresponding cell position at the
light path.
Delete the Input Value
CLEAR
Press to delete all the characters.
Delete the test results and stored data
CLEAR
Press to delete the test result or stored data.
Blank
ZERO
Put the Reference in the light path, press to do blank.
Measure Samples
9
S TART
S TOP
Put the samples in the light path, press to measure.
Print the test results
PRIN T
Press to print the test results.
Load the Standard Curve
“Quantitation” interface, Press , to select “Load Curve”, press
EN TER
, to choose the curve you want, press to open.
Measuring
1. Photometry
Step 1. Start Photometry
1
Main Interface, press numeric key or , to choose
EN TER
“Photometry”, then press .
Phot omet r y Phot omet r y
Quant i t at i on
0. 032A
Ki net i cs 01/ 01 500. 0nm
Ut i l i t y 00: 00
Step 2. Set Photometric Mode
S ET
Press to set photometric mode. Press , to choose
EN TER RETURN
“Abs.”, “T%” or “Energy”. And press to confirm. Press
to return.
Phot omet r y √Phot omet r y
Abs √
0. 032A
%T
500. 0nm
Ener gy
Step 3. Set Wavelength
10
GOTO ʎ
Press to set wavelength, key in the wavelength by using
EN TER
Numeric keys, press .
Step 4. Enter into Measuring Interface
S TART
S TOP
Press to enter into the Photometry Measuring Interface.
Phot omet r y 500. 0nm 0. 000A
No. WL Abs
0. 000Abs
500. 0nm
Step 5. Blank
ZERO
Put the Reference in the light path and press to do blank.
Step 6. Measure samples
Put the sample in the light path, and then the result displays on
S TART
S TOP
the screen automatically, press to record.
500. 0nm 0. 041A
No. WL Abs
1 500. 0 0. 039
2 500. 0 0. 042
3 500. 0 0. 041
2. Quantitation
Step 1. Start Quantitation
2
Main Interface, press or , to choose “Quantitation”,
EN TER
then press .
11
Phot omet r y √Quant i t at i on
Quant i t at i on St andar d Cur ve
Ki net i cs 01/ 01 Coef f i ci ent
Ut i l i t y 00: 00
Step 2. Establish or call Standard Curve
2 methods to establish Standard Curve:
Method 1: Establish Standard Curve by using Standard Samples
1) Starts establish. Press , to select “Standard Curve”,
EN TER
then press .
√Quant i t at i on St andar d Cur ve
St andar d Cur ve 0. 000Abs
Coef f i ci ent 0. 000mg/ ml
500. 0nm
GOTO ʎ EN TER
2) Set Wavelength. Press , input wavelength and press .
ZERO
3) Blank. Put the Reference in the light path, press to do
blank.
S ET
4) Enter into Set Interface. Press .
St andar d Cur ve √St andar d Cur ve
0. 000Abs Uni t
0. 000mg/ ml Number
500. 0nm Concent r at i ons
EN TER
5) Set Unit. Press , to select “Unit”, press , then
EN TER
press , to select Unit, press .
12
√St andar d Cur ve 1. % 5. mg/ l
Uni t 2. ug/ l 6. mg/ ml
Number 3. mg/ l 7. ug/ ml
Concent r at i ons 4. g/ l 8. None
6) Setup number of Standard Samples. Press , to select
EN TER
“Number”, setup number of Standard Samples, press to
confirm.
√St andar d Cur ve St andar d Cur ve
Uni t No=2
Number Pl ease ent er No
Concent r at i ons No=
7) Calibrate Standard Samples. Put the corresponding standard
samples in the light path as the screen indicates, input the
EN TER
concentration of the corresponding and press to measure.
√St andar d Cur ve Pl ease i nput 1# Sampl e
Uni t C=0. 000
Number Pl ease ent er Conc
Concent r at i ons C=
Method 2: Establish Standard Curve by inputting coefficients
1) Starts establish. Press , to select “Coefficient” ,
EN TER
select C=KxA+B, then press to confirm.
√Quant i t at i on C=KxA+B
St andar d Cur ve 0. 000Abs
Coef f i ci ent 0. 000mg/ ml
500. 0nm
S ET
2) Enter into Set Interface. Press .
13
C=KxA+B √Coef f i ci ent
0. 000Abs Coef f i ci ent K
0. 000mg/ ml Coef f i ci ent B
500. 0nm Uni t
3) Set test coefficient K and B. Press , to select
“Coefficient K” or “Coefficient B”, input coefficient K, press
EN TER
.
√Coef f i ci ent Coef f i ci ent
Coef f i ci ent K K=1. 000
Coef f i ci ent B Pl ease ent er Coe
Uni t K=
EN TER
4) Set Unit. Press , to select “Unit”, press , then
EN TER
press , to select Unit, press .
√Coef f i ci ent 1. % 5. mg/ l
Coef f i ci ent K 2. ug/ l 6. mg/ ml
Coef f i ci ent B 3. mg/ l 7. ug/ ml
Uni t 4. g/ l 8. None
GOTO ʎ EN TER
5) Set Wavelength. Press , input wavelength and press .
Load the Stored Curve
Press , to choose ”Select Curve”, press , to choose
EN TER
the cursor, press .
14
√St andar d Cur ve C=K×A+B
Concent r at i ons 001. C=1. 000×A+0. 003
Di spl ay Cur ve 002. C=2. 000×A+0
Sel ect Cur ve
Step 3. Enter into Measuring Interface
S TART
S TOP
Press to enter into the Quantitation Measuring Interface.
St andar d Cur ve 500. 0nm 0. 000A
No. WL Abs mg/ ml
0. 000Abs
0. 000mg/ ml
500. 0nm
Step 4. Blank
ZERO
Put the Reference in the light path, press to do blank.
Step 5. Measure Samples
S TART
S TOP
Put the sample to be tested in the light path, press to
measure. Then the test result will display in the data sheet. Repeat
this step to finish measuring all the samples.
500. 0nm 0. 000A
No. WL Abs mg/ ml
1 500. 0 0. 039 0. 078
2 500. 0 0. 042 0. 084
3 500. 0 0. 041 0. 082
3. Kinetics
Step 1. Start Kinetics
3
Main Interface, press or , to select “Kinetics” and
EN TER
press to confirm.
15
Phot omet r y Ki net i cs
Quant i t at i on
0. 000A
Ki net i cs 01/ 01 500. 0nm
Ut i l i t y 00: 00
Step 2. Set Wavelength
GOTO ʎ
Press to set wavelength, input wavelength by Numeric keys,
EN TER
and press to confirm.
Step 3. Setup Parameters
Press , to select values of “Time Intervals” , “Total Time”,
EN TER
“Test Mode”, “Upper Limited” and “Lower Limited”. Press to
confirm.
√Ki net i cs Ki net i cs
Ti me I nt er val T=1s
Tot al Ti me Pl ease ent er I nt er val
Test Mode T=
Step 4. Enter into Measuring Interface
S TART
S TOP
Press to enter into the Kinetics Measuring Interface.
Ki net i cs 100. 0
0. 000A 0
500. 0nm 0s 100s
500. 0nm 100. 0%T
Step 5. Blank
ZERO
Put the Reference in the light path, press to do blank.
Step 6. Measure Samples
S TART
S TOP
Put the sample in the light path and press to begin, repress
16
RETURN
it to stop, and press to cancel.
120. 0
0
0s 100s
500. 0nm 27s 100. 0%T
4. Utility
4
Main Interface, press or , to select “Utility” and press
EN TER
.
Phot omet r y √Ut i l i t y
Quant i t at i on D2 Lamp On
Ki net i cs 01/ 01 W Lamp On
Ut i l i t y 00: 00 Dat e&Ti me
Turn On/Off D2 Lamp
EN TER
Press , to choose “D2 Lamp”, then press to enter into.
EN TER
Press , to choose “On” or “Off”, press to turn on/off.
√Ut i l i t y √D2 Lamp
D2 Lamp On On √
W Lamp On Of f
Dat e&Ti me
Turn On/Off W Lamp
EN TER
Press , to choose “W Lamp”, then press to enter into.
EN TER
Press , to choose “On” or “Off”, .press to turn on/off.
17
√Ut i l i t y √W Lamp
D2 Lamp On On √
W Lamp On Of f
Dat e&Ti me
Set Date & Time
EN TER
Press , to choose “Date & Time”, then press to enter
EN TER
into. Input hh:mm:ss and yy-mm-dd, .press to confirm.
√Ut i l i t y √Dat e&Ti me
D2 Lamp On Ti me: 00: 00: 00
W Lamp On Dat e: 2000- 01- 01
Dat e&Ti me
Get Dark Current
Keep the light path without anything blocking, press , to
EN TER
choose “Dark Current”, then press to resample Dark Current.
Note: During the course, open the lid of the compartment is prohibited.
√Ut i l i t y
Dar k Cur r ent
Get Dar k Cur r ent . . .
Reset WL
Lamp Li f e
Reset Wavelength
Keep the light path without anything blocking, press , to
EN TER
choose “Reset WL”, then press to reset wavelength.
18
√Ut i l i t y
Dar k Cur r ent
Cal i br at i ng WL. . .
Reset WL
Lamp Li f e
Lamp Life
EN TER
Press , to choose “Lamp Life”, then press to enter into.
Select “Display Lamp Life” to view the W Lamp and D2 Lamp has been
used time. Select “Reset D2 Life” or “Reset W Life” to reset the time.
√Ut i l i t y √Lamp Li f e
Dar k Cur r ent Di spl ay Lamp Li f e
Reset WL Reset D2 Li f e
Lamp Li f e Reset W Li f e
Load Default Parameters
EN TER
Press , to choose “Load Default”, then press to enter
into. Select “Yes” to load the parameters to factory setting and the
instrument will restart.
√Ut i l i t y √Load Def aul t
Load Def aul t Yes
Lamp Change No
Ver si on
Lamp Change
EN TER
Press , to choose “Lamp Change”, then press to enter
EN TER
into. Input switch wavelength (325—375nm)and press to confirm.
19
√Ut i l i t y Lamp Change
Load Def aul t WL=339. 0nm
Lamp Change Pl ease ent er WL
Ver si on WL=
About Version
EN TER
Press , to choose “Version”, press to view version
information, press any key to return.
√Ut i l i t y
Load Def aul t Sof t war e: V1. 4. 2
Lamp Change Har dwar e: V1. 0
Ver si on
Troubleshooting
Review the information in the table below to troubleshoot operating
problems.
Problem Cause Solution
Power cord connection
Improve connectivity
Power on, no response is not reliable
Fuse burning Replace fuse
Warm up is not enough Warm up more time
Glass cuvettes used in
Use quartz cuvettes
UV Range
Sample is not Stable Improve the sample
The concentration of
Measurement uncertainty Diluted sample
sample is too high
Power Supply Voltage Improve the Power
Low or not Stable Supply
Lamp damage or lamp life
Replace lamp
maturity
The lid of the
Dark Current Error when
compartment is open Close the lid, restart
self-check
during self-check
20
Something block the Remove it, calibrate
System Calibrate Failed
Light path again
Power on, back light is
OK, but nothing display Display Contrast Adjust the contrast
on the screen or display problem potentiometer
is not clear
Cuvettes were
Clean cuvettes
contaminated
Samples were
Improve samples
Measurements inaccurate contaminated
Worse matching of the Improve the matching of
cuvettes the cuvettes
Dark current error Resample dark current
Repair and Maintenance
1. Daily Maintain
Check the Compartment
After measurement, the cuvettes with sample solutions should be taken
out of the compartment in time. Or the volatilization of the solution
would make the mirror go moldy. Users must pay more attention to the
corrosive sample and liquid easy to volatilize. Any solution remains
in the compartment should be wiped off immediately.
Surface Clean
The cover of the instrument is with paint. Please use wet towel to
wipe off the drips on the surface immediately. Organic solution is
forbidden to be used to clean the cover. Please wipe off the dirt on
the cover timely.
Clean the Cuvettes
After every test or after a solution change, the cuvettes should be
cleaned carefully, or the remains on the surface would cause measuring
error.
2. Spare Parts Replacement
Replace Fuse
Danger! Be sure to switch off the power and unplug the
socket before replacement!
21
Step 1. Tools preparation
Prepare a 3×75 Flat Blade screwdriver.
Step 2. Switch Off the power supply
Switch off the power supply, and unplug the socket.
Step 3. Take out the Fuse Seat
Take out the fuse seat by the screwdriver.
Step 4. Replace a new fuse
Pick out the spare fuse (3.15A/250V) and replace it to the working
position.
Step 5. Reset the fuse seat
Replace the fuse seat in the power socket.
Step 6. Switch on the power
Plug the socket and switch on the power.
Replace Lamps
Hot! Wait 20 minutes before open the lamp chamber after
power off to avoid scald!
22
Step 1. Tools preparation
Prepare a 6×150mm Cross Blade screwdriver and a pair of glove.
Step 2. Power Off
Switch off the power supply and unplug the socket.
Step 3. Open the cover
Unscrew the 4 screws indicated(Each side with 2 screws) and remove
the cover.
Step 4. Open the cover of the light chamber
Unscrew the 2 screws on the light chamber cover and remove it.
Light
chamber
Step 5. Replace the D2 lamp (Only for model UV-1600/UV-1800)
Unscrew the 2 screws on the D2 Flange (No.1), unplug the connector
in the Power Board (No. 2) and remove the D2 lamp. Draw on the
cotton glove and replace a new lamp. Fix the 2 screws and plug the
connector again.
23
D2
Lamp
1
2
Step 6. Replace W lamp
Pull out the defected W lamp and draw on the cotton glove. Insert
the new W lamp as deep as possible on the lamp seat. Be sure to
keep the filament in the same direction as the old one face.
W
Lamp Filament
Step 7. Adjust the position of the W lamp
Switch on the power(the Switch Mirror should be placed to the
position as indicates). Observe the entrance facular, and it should
in the center of the entrance hole. If the facular deviate to Left
or Right, then loosen the No.1 screws in Fig. 5-8 and move the lamp
seat to Left or Right until it focus on the center of the slot.
Then fix the screws. If the facular deviate to Up and Down, then
loosen the No.2 screws and move the lamp seat Up and Down until the
facular focus on the center of the slot. Then fix the No. 2 screws
again.
24
Right Position
Switch
Mirror
2
Step 8. Finish
Reset the cover of the light chamber and fix the screws. Reset the
cover of the instrument and fix the screws. Recover the Pole in the
compartment, then the course finished.
Replace the Battery
Danger! Be sure to switch off the power and unplug the
socket before replacement!
Step 1. Prepare the tools
Prepare a 6×150mm Cross Blade Screwdriver.
Step 2. Switch off the power supply
Switch off the power supply and unplug the socket.
Step 3. Open the Bottom cover plate
Unscrew all the screws indicated then remove the bottom plate.
25
Step 4. Replace the Battery
Pick out the old battery and replace a new one.
Step 5. Finish
Recover the bottom plate and fix all the screws, then the course
finishes.
Warranty
We warrant that this product will be free from defects in material and
workmanship for a period of one (1) year from date of purchase. If a
defect is present, we will, at its option, repair, replace, or refund
the purchase price of this product at no charge to you, provided it is
returned during the warranty period. This warranty does not apply if
the product has been damaged by accident, abuse, misuse, or
misapplication, or from ordinary wear and tear.
For your protection, items being returned must be insured against
possible damage or loss. This warranty shall be limited to the
replacement of defective products. IT IS EXPRESSLY AGREED THAT THIS
WARRANTY WILL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF
THE WARRANTY OF MERCHANTABILITY.
Equipment Disposal
26
This equipment is marked with the crossed out wheeled bin symbol to
indicate that this equipment must not be disposed of with unsorted
waste.
Instead it's your responsibility to correctly dispose of your equipment
at lifecycle -end by handling it over to an authorized facility for
separate collection and recycling. It's also your responsibility to
decontaminate the equipment in case of biological, chemical and/or
radiological contamination, so as to protect from health hazards the
persons involved in the disposal and recycling of the equipment.
For more information about where you can drop off your waste of
equipment, please contact your local dealer from whom you originally
purchased this equipment.
By doing so, you will help to conserve natural and environmental
resources and you will ensure that your equipment is recycled in a
manner that protects human health.
Thank you!
27
