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Antibacterial Activity of Peppermint Extract

The document describes a methodology for testing the antibacterial activity of peppermint extract. Three bacterial strains - one Gram-positive and two Gram-negative - were selected. The bacteria were grown on agar plates and discs impregnated with peppermint extract or control substances were placed on the plates. The plates were incubated and the diameter of inhibition zones around the discs was measured, with larger zones indicating greater antibacterial activity of the peppermint extract. References about previous studies on the antibacterial activity of peppermint extracts are also provided.

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60 views2 pages

Antibacterial Activity of Peppermint Extract

The document describes a methodology for testing the antibacterial activity of peppermint extract. Three bacterial strains - one Gram-positive and two Gram-negative - were selected. The bacteria were grown on agar plates and discs impregnated with peppermint extract or control substances were placed on the plates. The plates were incubated and the diameter of inhibition zones around the discs was measured, with larger zones indicating greater antibacterial activity of the peppermint extract. References about previous studies on the antibacterial activity of peppermint extracts are also provided.

Uploaded by

Sara Juita
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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METHODOLOGY

Bacterial strains

One Gram-positive (Staphylococcus aureus) and two Gram-negative (Pseudomonas


aeruginosa and Klebsiella pneumonia) bacteria were selected for antibacterial activity assay.

Antimicrobial activity assay by disc diffusion method

The peppermint extract was dissolved in ethanol to a final concentration of 1 mg/ml. The
bacterial strains were grown on nutrient agar (NA) by spread plate method. The bacteria were
pipetted onto surface of agar plate (0.1 ml or less) and were spread evenly over surface of
agar using sterile glass spreader. Blank discs were impregnated with peppermint extract using
ethanol. The discs were allowed to dry at room temperature before placed on the agar plate. A
disc impregnated with distilled water was used as a negative control. Bacitracin disc,
cefepime disc and ampicillin disc was used as a positive reference standard to determine the
sensitivity of the bacterial strains respectively. The plates were incubated at 37°C for 24 h.
Antimicrobial activity was evaluated by measuring the diameter zone of inhibition around the
disc. The resultant clear zones around the discs were measured in mm. The antibacterial
activity of peppermint extracts were indicated by clear zones of growth inhibition.

REFERENCES

Bupesh, G., Amutha, C., Nandagopal, S., Ganeshkumar, A., Sureshkumar, P., & Murali, K.
(2007). Antibacterial activity of Mentha piperita L. (peppermint) from leaf extracts - a
medicinal plant. Acta Agriculturae Slovenica, 89(1), 73-79. doi:10.2478/v10014-007-
0009-7

Basheer, A. A., & Abdullah, A. A. (2013). Antimicrobial activity of the aqueous extract of
mint plant. Science Journal of Clinical Medicine, 2(3), 110-113.
doi:10.11648/j.sjcm.20130203.19

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