Thyroid Function Ordering Algorithm
Nonhospitalized patients without
known or suspected pituitary disease
Begin evaluation by ordering either the cascade approach
THSCM / Thyroid Function Cascade, Serum
(all appropriate tests are performed automatically)
or order each test individually, beginning with
STSH / Thyroid-Stimulating Hormone-Sensitive (s-TSH), Serum
s-TSH
Functional sensitivity: 0.01 mIU/L
<0.10 mIU/L* 0.100.29 mIU/L* 0.304.2 mIU/L* >4.2 mIU/L*
Hyperthyroid suspect Borderline low TSH No further testing Hypothyroid suspect
unless clinically indicated
Order Free T4: Order Free T4: Order Free T4 and TP0:
FRT4 / T4 (Thyroxine), FRT4 / T4 (Thyroxine), FRT4 / T4 (Thyroxine), Free, Serum
Free, Serum Free, Serum TPO / Thyroperoxidase (TPO)
Antibodies, Serum
If Free T4 is normal
Order Total T3:
T3 / T3 (Triiodothyronine),
Total, Serum
* Adult s-TSH reference ranges. See Laboratory Test Catalog for pediatric reference ranges.
In patients receiving therapy with high biotin doses (>5 mg/day) there should be an interval
of at least 8 hours between the last biotin administration and collection of the sample.
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