Tissues Tissue = collection of specialized cells that

(Chapter 4) perform limited number of functions

Lecture Materials
Histology = the study of tissues
for
Four Main Types of Tissue:
Amy Warenda Czura, Ph.D.
1. Epithelial tissue ! covering
Suffolk County Community College surfaces, linings, glands
2. Connective tissue ! support
Eastern Campus
fill space, structure, strength, transport,
storage
Primary Sources for figures and content: 3. Muscle tissue ! movement
Marieb, E. N. Human Anatomy & Physiology 6th ed. San Francisco: Pearson Benjamin
Cummings, 2004. 4. Nervous tissue ! control
Martini, F. H. Fundamentals of Anatomy & Physiology 6th ed. San Francisco: Pearson
Benjamin Cummings, 2004.
transmit info

Primary Germ Layers =embryonic layers, give

rise to all four tissue types in adult

Features:
1. Cellularity: little extracellular matrix,
mostly cells
2. Contacts: cells linked by strong junctions
3. Polarity: apical + basal surfaces, separate
functions
4. Attachment: attached to CT via basal
lamina
1. Ectoderm: nervous, epithelial (epidermis)
5. Avascularity: diffusion from CT
2. Mesoderm: muscle, connective, epithelial
6. Regeneration: high turnover, stem cells at
(endothelium + mesothelium)
basal surface
3. Endoderm: epithelial (mucosa)
Functions:
1. Provide physical protection:
abrasion, dehydration, infection
Epithelial Tissue
2. Control permeability:
2 categories:
semi-permeable, covers all surfaces
1. Epithelia - covering
3. Provide sensation:
2. Glands -produce fluid secretions
sensory neurons
4. Produce specialized secretions: (glandular)
protection, chemical messengers

Amy Warenda Czura, Ph.D. 1 SCCC BIO130 Chapter 4 Lecture Notes

 Intercellular Connections (on handout)

Structure
1. Apical surface: exposed to environment
may have:
microvilli: absorption or secretion
cilia: fluid movement
2. Basolateral surface: attachment to
neighboring cells via intercellular
connections

Classification (based on shape and layers)

Shape: (all are hexagonal from the top)
1. Squamous: flat, disc shaped nucleus
2. Cuboidal: cube, center round nucleus
3. Columnar: tall, basal oval nucleus

Basal lamina (a.k.a. basement membrane)

1. Lamina lucida: from epithelia
glycoproteins + fine filaments
restrict large molecule movement
2. Lamina densa: from CT
coarse protein fibers
provide strength
Layers:
1. Simple: one cell layer
absorption, secretion, filtration
2. Stratified: 2 or more
protection
In stratified, name for apical cell shape
Epithelial stem cells anchored to lamina lucida:
divide and migrate toward apical region

Amy Warenda Czura, Ph.D. 2 SCCC BIO130 Chapter 4 Lecture Notes

Types of Epithelial Tissue 2. Stratified Squamous Epithelium
-basal cells look cuboidal, apical cells
1. Simple Squamous Epithelium squamous
-thin, delicate -on exposed surfaces
-in protected regions -two types:
-Locations: A. Nonkeratinized = mucosa
-mesothelium -kept moist
(serosa) -all cells nucleated
-endothelium Locations:
(blood vessels, heart) -mouth, esophagus
-kidney tubules -anus
-conjunctiva -vagina
-alveoli of lungs B. Keratinized = epidermis
-Functions: -dry, apical cells dead
absorption, diffusion, filtration or -cells contain keratin protein
secretion to resist dehydration
-Functions:
provide protection from abrasion,
pathogens, chemicals

3. Simple Cuboidal Epithelium 5. Transitional Epithelium

-Locations:
-kidney tubules
-pancreas
-salivary glands
-thyroid
-Functions:
-secretion or absorption -relaxed: looks like stratified cuboidal
-stretched: looks squamous
4. Stratified Cuboidal Epithelium -Locations:
-rare -urinary bladder
-typically 2 layers -ureters
-Locations: -Function:
-some sweat glands -tolerate excessive stretching
-some mammary glands
-Functions:
-secretion or absorption

Amy Warenda Czura, Ph.D. 3 SCCC BIO130 Chapter 4 Lecture Notes

6. Simple Columnar Epithelium 7. Pseudostratified Columnar Epithelium
Brush border

-several cell types:

May have goblet cells for mucus secretion
varying shapes and functions
-nuclei line up near basal lamina -all cells contact basal lamina
-apical surface of cells often has microvilli = -some too short to reach apical surface
brush border (in intestine) -nuclei scattered so it appears stratified
-goblet cells often present: secrete mucus -tall cells have cilia on apical surface
-Locations: -goblet cells (mucus) often present
-stomach -Locations:
-intestine -nasal cavity, trachea, bronchi
-gallbladder -male reproductive tract
-uterine tubes -female uterine tubes
-collecting ducts of kidney -Functions:
-Functions: -move material across surface
-absorption or secretion

8. Stratified Columnar Epithelium Glandular Epithelium

for secretion, makes up glands

1. Endocrine glands: internally secreting

secrete into interstitial fluid ! blood
-rare secretions = hormones
-two layers or multiple layers with only regulate and coordinate activities
apical layer columnar e.g. pancreas, thyroid, thymus, pituitary
-Locations (tiny parts of):
-pharynx, epiglottis 2. Exocrine glands: externally secreting
-anus secrete into duct ! epithelial surface
-mammary glands e.g. digestive enzymes, perspiration,
-salivary glands tears, milk, mucus
-urethra Classified three ways:
-Functions a. mode of secretion
-minor protection b. type of secretion
c. structure

Amy Warenda Czura, Ph.D. 4 SCCC BIO130 Chapter 4 Lecture Notes

A. Mode of secretion B. Type of secretion
1. Merocrine secretion 1. Serous glands: water + enzymes
-product released from e.g. parotid salivary gland
secretory vesicles 2. Mucus glands: mucin
by exocytosis (+water = mucus)
e.g. mucus, sweat e.g. goblet cell
2. Apocrine secretion 3. Mixed exocrine glands:
-product accumulates in vesicles serous + mucus secretion
-apical region of cell with vesicles is shed e.g. submandibular salivary gland
to release product
e.g. milk C. Gland structure
1. Unicellular gland: 1 cell
3. Holocrine secretion e.g. goblet cell
-product accumulates in vesicles 2. Multicellular gland: group of cells
-whole cell is lysed to release product named for shape and structure
-cell dies, must be replaced by stem cells
e.g. sebum

Connective Tissue Functions

Features: 1. Establish structural framework
-never exposed to environment 2. Transport fluid and dissolved materials
-usually vascularized 3. Protect organs
-consists of cells in a matrix 4. Support, surround, interconnect tissues
5. Store energy reserves
Components: 6. Insulate body
1. Specialized cells: 7. Defend against pathogens
produce matrix, provide protection
2. Extracellular protein fibers: Classification: based on physical properties
support, strength 1. Connective Tissue Proper:
3. Ground substance: many cell types and fiber types in thick
gel fluid, consists of: ground substance
interstitial fluid, A. Loose: open fiber framework
CAMs, B. Dense: tightly packed fibers
GAGs (glycosaminoglycans) 2. Fluid Connective Tissue:
proteoglycans that gel many cell types in watery matrix with
soluble fibers
Fibers + Ground substance = Matrix 3. Supporting Connective Tissue:
limited cell population in tightly packed
matrix

Amy Warenda Czura, Ph.D. 5 SCCC BIO130 Chapter 4 Lecture Notes

 1. Connective Tissue Proper
-viscous ground substance
-varied extracellular fibers
-varied cell population
Ground substance: rich in GAGs
-viscous, prevents microbe penetration

Fiber types:
All CT derived from Mesenchyme: 1. Collagen fibers: collagen protein
embryonic CT: mesenchymal cells in -rope like, long, straight
gelatinous matrix with fine fibers -resists force
-most common
2. Reticular fibers: collagen protein
-branchy, forms framework
framework of an organ = stroma
(functional cells of an organ =
parenchyma)

3. Elastic fibers: elastin protein

-wavy, flexible
-designed to stretch

Cell Types: 3. Adipocytes (fat cells)

1. Fibroblasts -store triglycerides
-most common, most abundant -organelles pushed to periphery
-secrete ground substance: -number, size and location of cells varies
hyaluronan + proteins = GAGs 4. Macrophages
-secrete fiber proteins (collagen, elastin) -phagocytic for defense
-some specialized types: -some fixed in tissues
chondrocytes (cartilage) -others migrate from blood to tissues
osteocytes (bone) after injury
2. Mesenchymal cells 5. Microphages
-stem cells -neutrophils and eosinophils
-differentiate to replace CT cells after -phagocytic
injury (e.g. fibroblasts, adipocytes) -migrate from blood to site of injury

Amy Warenda Czura, Ph.D. 6 SCCC BIO130 Chapter 4 Lecture Notes

 A. Loose Connective Tissue
-highly vascularized
-varied cell types
-functions:
fill space
cushion & support tissues
store fat
feed epithelial layers
1. Areolar CT
-most common
6. Lymphocytes: B and T cells -least specialized
-involved in immune response -open framework:
-make antibodies, attack foreign cells matrix mostly ground substance
-increase in number during infection -all fiber types
-constantly migrate between blood and Location:
tissues and lymph -deep to epithelium
7. Mast cells Functions:
-contain histamine and heparin -reservoir for water & salts
-stimulate inflammation in response to -absorbs shock & distortion
injury -fills space
-feeds epithelium

2. Adipose Tissue B. Dense Connective Tissue

-90% adipocytes -poorly vascularized
Locations: -mostly fibers, little ground substance
-deep to skin -only fibroblasts
-surrounding 1. Dense Regular CT
eyeballs, kidneys, heart -bundles of parallel collagen fibers,
Functions: aligned with direction of force
-padding & insulation
-energy storage
-two types:
a. white fat: adults, triglyceride storage Locations:
b. brown fat: infants, high mitochondria -tendons (muscle to bone)
content for heat generation -ligaments (bone to bone)
3. Reticular Tissue -muscle coverings
-stroma of organs -fascia
-consists of Function:
reticular fibers -high strength attachment
Locations: some organs: -stabilize positions
e.g. lymph nodes, bone marrow, liver
Function:
support parenchyma cells

Amy Warenda Czura, Ph.D. 7 SCCC BIO130 Chapter 4 Lecture Notes

 2. Dense Irregular CT 2. Fluid Connective Tissue
-mesh of collagen fibers A. Blood

Matrix = plasma: serum(fluid)

Locations: + plasma proteins (produced by liver)
-capsules of organs & fascia fibers are soluble until clot forms
-periosteum (sheath around bone) Cells = formed elements
-perichondrium (around cartilage) originate from hemocytoblast (stem cell)
-dermis (deep skin) in bone marrow
Function: Formed Elements:

-resist tension from many directions B cells

T cells
-attachment
3. Elastic CT Erythrocytes
(Macrophage)
(Mast cell)

-mostly elastic fibers, Leukocytes

some collagen 1. Erythrocytes (RBCs): carry O2

Locations: 2. Leukocytes (WBCs): defense
-vertebral ligaments Neutrophils, Eosinophils, Basophils,
-artery walls Lymphocytes (B and T cells),
Function: Monocytes (Macrophages)
-strength with stretch and flex 3. Platelets: carry clotting factors

Location: 3. Supporting Connective Tissue

-contained in blood vessels -strong framework, few cells, fibrous matrix
Function: -function: support and shape
-transport nutrients, wastes and defense -mature cells in lacunae
cells throughout body
A. Cartilage
Plasma! Interstitial fluid! Lymph! Plasma Composition:
-Matrix: 80% water, firm gel of GAGs
B. Lymph made of chondroitin sulfate and
Matrix = lymph (recollected plasma fluid) hyaluronic acid, + fibers
Cells = lymphocytes (immune defense) -Cells: chondrocytes (in lacunae)
Location: contained in lymphatic vessels (cells formed the matrix)
Function: purify and return fluid to blood Structure:
-no innervation
-avascular (antiangiogenesis factor)
-surrounded by perichondrium:
outer layer = dense irregular CT
protection, attachment
inner layer = cellular (fibroblasts)
growth and repair

Amy Warenda Czura, Ph.D. 8 SCCC BIO130 Chapter 4 Lecture Notes

Growth of cartilage: (not common in adults) Types of cartilage:
1. Interstitial growth (embryos) 1. Hyaline Cartilage
-chondroblasts in matrix divide -matrix contains fine, closely packed
-daughters produce more matrix collagen fibers
(mature cells = chrondrocytes) -tough, springy
Locations:
-ribs
-nose
-respiratory tract
2. Appositional growth (children, minor -articular surfaces (no perichondrium)
repair in adult) Function:
-new layers added by cells of inner -provide stiff flexible support
perichondrium -reduce friction between bones
2. Elastic Cartilage
-matrix contains elastic fibers
-flexible
Locations:
-auricle of ear
-epiglottis
Serious injury = scar: cartilage replaced by Function:
fibrous collagen -resilient, flexible, shape holding support

3. Fibrocartilage
-matrix contains dense interwoven collagen B. Bone / Osseous Tissue
fibers with little ground substance -highly vascularized
-tough, durable -little ground substance

-matrix = 2/3 calcium salts for strength

(calcium phosphate + calcium carbonate)
1/3 collagen for flexibility to resist shatter
Locations: -cells = osteocytes
-knee (meniscus) (cells formed the matrix)
-pubic symphasis -located in lacunae
-intervertebral discs -connected by cytoplasmic extensions
Functions: that extend through canaliculi
-resist compression -canaliculi necessary for nutrient & waste
-absorb shock exchange, no diffusion through calcium
-surrounded by periosteum:
outer fibrous layer for attachment
inner cellular layer for growth and repair
Location:
bones

Amy Warenda Czura, Ph.D. 9 SCCC BIO130 Chapter 4 Lecture Notes

 Functions: Three layers:
-support & protection A. Superficial fascia
-levers for movement -located between cutaneous membrane and
-storage of minerals organs
-composed of areolar CT and adipose
Special Connective Tissue Structures -a.k.a. subcutaneous layer/hypodermis
1. Fascia -functions to provide insulation and padding
-Connective tissue that provides a framework
to connect organs to the body
-Functions:
- provide strength and stability
-maintain the position of the organs
-anchor blood vessels, lymphatic vessels and
nerves
B. Deep fascia
-forms strong framework of dense CT
-creates capsules around organs, binds
capsules, tendons, ligaments to hold tissues
together
-functions to resist force and anchor
positions of organs

C. Subserous fascia
-between serous membranes and deep fascia
-composed of areolar CT
-functions to prevent distortion of organs by
muscles

2. Membranes B. Serous Membranes / Serosa

-typically epithelium plus supporting CT -mesothelium + areolar connective tissue
A. Mucous Membranes / Mucosa -lines body cavities to reduce friction
-epithelium + areolar connective tissue (pleura, peritoneum, pericardium)
called lamina propria -parietal layer lines cavity, visceral layer
-line passageways that connect to external around organ
environment -epithelium kept moist by serous fluid /
-epithelium kept moist with mucus secretions transudate

Amy Warenda Czura, Ph.D. 10 SCCC BIO130 Chapter 4 Lecture Notes

C. Cutaneous Membrane Muscle Tissue
(epidermis + dermis = skin) Function:
-keratinized stratified squamous epithelium -produce movement
+ areolar and dense irregular connective Features:
tissue - highly vascularized
-only dry membrane - contains actin and myosin for contraction
Types:
1. Skeletal Muscle (Striated Voluntary Muscle)
-cells = fibers
-up to 1 ft long
-multinuclear
-no cell division
-appear striated: actin and myosin
D. Synovial Membranes organized into myofibrils
-areolar connective tissue with woven -some satellite cells (stem cells) present
collagen, proteogycans and glycoproteins for minor repair after injury
in matrix Location: skeletal muscles (meat)
-lines joint capsules Functions:
-produces synovial fluid to reduce friction -move skeleton
of articular cartilage -guard entrances/exits
-only membrane with no epithelium -generate heat

2. Cardiac Muscle 3. Smooth Muscle

(Striated Involuntary Muscle) (Nonstriated Involuntary Muscle)
-cells = cardiocytes
-long, branched
-usually single nucleus
but up to 5
-no cell division -cells small, spindle shaped
-striated -uninuclear
-neighboring cells connected by -capable of cell division
intercalated discs: desmosomes + -no striations: no myofibril organization
intercellular cement + gap junctions Locations:
*desmosomes & cement provide tight -walls of blood vessels
linkage of neighboring cells -walls of hollow organs:
*gap junctions allow exchange of ions digestive, respiratory, urinary and
to coordinate contraction reproductive tracts
Function:
-move materials through the organ

Location: majority of heart

Function: move blood through body

Amy Warenda Czura, Ph.D. 11 SCCC BIO130 Chapter 4 Lecture Notes

Nervous Tissue Tissue Injuries
Function:
-maintain homeostasis:
-conduct nervous impulses to relay
information inflammation and repair
Location:
Inflammation:
-most in brain and
- prevent spread of injury or infection
spinal cord: CNS
- characterized by: swelling, redness, warmth
-2% in PNS
and pain
Cells:
- process to remove necrotic cells and
1. Neuroglia cells
infectious agents
-support, repair, and supply nutrients to
(inflammation process on handout)
nervous tissue
2. Neurons
-transmit information
-up to 3 ft long
-large soma (cell body)
-large nucleus, visible nucleoli
-many dendrites: receive info
-one axon: transmits info
-no cell division

Injury and Cancer:

-repeat or chronic
inflammation causes damage

Dysplasia: change in normal

shape, size, organization of
tissue cells (reversible)

Metaplasia: more serious

changes, abnormal division
of stem cells (reversible)

Anaplasia: breakdown of
tissue organization, genetic
abnormalities of stem cells
(irreversible)

Cancer = uncontrolled growth

Amy Warenda Czura, Ph.D. 12 SCCC BIO130 Chapter 4 Lecture Notes