ALAT / GPT
IFCC
Cat.No
142 016
142 000
142 001
142 002
142 003
142 008
142 004
142 007
142 025
Package Size
7 x 10 mL
5 x 20 mL
2 x 50 mL
4 x 100 mL
4 x 50 mL
4 x 80 mL
4 x 100 mL
4 x 70 mL
5 x 20 mL
(Hit I)
(Hit II)
(Hit II)
(AU)
(LW)
2 x 7 mL
2 x 10 mL
1 x 20 mL
4 x 20 mL
2 x 20 mL
4 x 16 mL
4 x 20 mL
3 x 20 mL
2 x 10 mL
METHOD
PRINCIPLE
Kinetic determination of Alaninaminotransferase
(ALAT) based upon IFCC recommendations:
Pyruvat + NADH + H+
Note: It is recommended for each laboratory to establish and
maintain its own reference values. The given data are only an
indication.
PROCEDURES
Reagents can be used manually (see method below) and
on most analyzers. Applications are available on request.
Wavelength
:
Temperature
:
Cuvette
:
Read against air.
Optimized, UV, Kinetic.
L-Alanin+ 2-Oxoglutarat
REFERENCE VALUES (IFCC / 37C)
Women
< 31 U/L
Men
< 41 U/L
ALAT > L-Glutamat + Pyruvat
< LDH > L-Lactat + NAD+
ALAT = Alaninaminotransferase
LDH = Lactate dehydrogenase
REAGENTS COMPOSITION
R1:
TRIS-Buffer (pH 7,5)
100 mmol/l
R2:
L-Alanine
LDH ( Lactatdehydrogenase)
2-Oxoglutarate
500 mmol/l
1200 U/l
15 mmol/l
NADH
0,18 mmol/l
- PRECAUTIONS
- For in vitro diagnostic use only.
- The reagents contain < 0,95g/L sodium azide.
Avoid contact with skin and/or mucous
membranes !
- Use clean or single use glass/plastic material
only to avoid contaminations.
STABILITY OF REAGENTS
When stored at 2 8 C and protected from light,
the reagents are stable until the expiry date printed
on the labels.
PREPARATION OF WORKING REAGENT
- For Start Reagent-procedure
reagents R1 and R2 are ready for use
- For Sample Start-procedure (monoreagent)
mix R1 + R2 = 5 + 1
Stability : 1 month at 2 8 C
5 days at 18 25 C
340 nm, Hg 334 nm, Hg 365 nm
37C,
1 cm light path
Sample Start Procedure / Monoreagent
Working reagent :
Sample :
1 mL
100 L
Mix and after a 1 minute incubation, measure the
change of absorbance per minute (A/min) during
3 minutes.
Start Reagent Procedure/R1-R2
R1 :
Sample :
Mix and wait 1 minute
1 mL
100 L
R2
:
200 L
Mix and after a 1 minute incubation, measure the
change of absorbance per minute (A/min) during
3 minutes.
CALCULATION FACTORS
Monoreagent
340 nm : Activity (U/L) = A/min. x 1 745
334 nm : Activity (U/L) = A/min. x 1 780
365 nm : Activity (U/L) = A/min. x 3 235
Start reagent
2064
2103
3824
CALIBRATORS & CONTROLS
For the calibration of automated analyzers Greiner
Multicalibrator is recommended, for quality control
use Greiner normal and abnormal control, Unitrol I
and Unitrol II
SAMPLES
Serum free of hemolysis , plasma (EDTA/heparin)
Page 1/2
Greiner Diagnostic GmbH - Unter Gereuth 10 D-79353 Bahlingen Germany
www.greiner-diagnostic.com
PERFORMANCE DATA (37C)
- Analytical range
The reagent is linear up to ALAT activities
corresponding to a A/min of 0,16 at 340 nm and
334 nm,which corresponds to 1300U/L. If A/min
exceeds 0.16 at 340 nm, repeat the test using
serum diluted 1/10 with sodium chloride solution (9
g/L).Multiply result by 10.
BIBLIOGRAPHY
- Sensitivity
The sensitivity / lower detection limit is < 1U/L
3.
1.
2.
- Precision
Within-run reproducibility
Within series
Mean
n = 11
Value
[U/L]
Sample 1
38,4
Sample 2
143
Sample 3
111
Standarddeviation
[U/L]
0,51
1,95
1,27
Between-run reproducibility
Day to day
Mean
n = 11
Value
[U/L]
Sample 1
36,0
Sample 2
146
Sample 3
109
Standarddeviation
[U/L]
0,78
0,94
1,40
CV
[%]
1,32
1,36
1,14
4.
Thomas L. Alanine aminotransferase (ALT),
Aspartate aminotransferase (AST). In: Thomas L,
editor. Clinical Laboratory Diagnostics. 1st ed.
Frankfurt: TH-Books Verlagsgesellschaft; 1998. p.
55-65.
Moss DW, Henderson AR. Clinical enzymology. In:
Burtis CA, Ashwood ER, editors. Tietz Textbook of
Clinical Chemistry. 3rd ed. Philadelphia: W.B
Saunders Company; 1999. p. 617-721.
Bergmeyer HU, Horder M, Rej R. International
Federation of Clinical Chemistry (IFCC) Scientific
Committee,
Analytical
section:
approved
recommendation (1985) on IFCC methods for the
measurement of catalytic concentration of enzymes.
Part 3. IFCC method for alanine aminotransferase
(L-alanine:2-oxoglutarate aminotransferase, EC
2.6.1.2). J Clin Chem Clin Biochem 1986;24:481-95.
Lorentz K, Rhle G, Siekmann L. Einfhrung der
neuen Standardmethoden 1994 zur Bestimmung der
katalytischen Enzymkonzentrationen bei 37 C. DG
Klinische Chemie Mitteilungen 1995;26:190-2.
CV
[%]
2,15
0,65
1,28
SYMBOLS USED
- Correlation
A comparative study has been perfo ssion are as
follows:rmed between the Greiner method and
another commercial reagent on 30 human serum
samples. The parameters of linear regre
For in vitro diagnostic medical use
Correlation coefficient
Linear regression:
Use by
r = 0.998
y = 0,967x + 3,365 U/L
Batch Code
Temperature limitation
INTERFERENCES
- Ascorbic Acid: no interference until 100 mg/L
- Triglycerides: no interference until 1000 mg/dL
- Bilirubin:
no interference up to 40 mg/dL
- Hemoglobin:
no interference up to 400 mg/dL
06/2013//rev03/2014
Page 2/2
Greiner Diagnostic GmbH - Unter Gereuth 10 D-79353 Bahlingen Germany
www.greiner-diagnostic.com
