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Most Probable Number

This document describes the Most Probable Number (MPN) method for detecting and quantifying total coliforms, E. coli, and fecal coliforms in water samples using Colilert-18. Colilert-18 uses defined substrate technology to detect these bacteria through color and fluorescence changes when metabolized. It can simultaneously detect bacteria at concentrations as low as 1 cfu/100mL. The document outlines both the presence/absence and Quanti-Tray enumeration procedures, which involve adding the Colilert-18 reagent to a water sample, incubating, and reading results to determine if bacteria are present or quantify the MPN.

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Sreedhar Reddy Sajjala
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120 views1 page

Most Probable Number

This document describes the Most Probable Number (MPN) method for detecting and quantifying total coliforms, E. coli, and fecal coliforms in water samples using Colilert-18. Colilert-18 uses defined substrate technology to detect these bacteria through color and fluorescence changes when metabolized. It can simultaneously detect bacteria at concentrations as low as 1 cfu/100mL. The document outlines both the presence/absence and Quanti-Tray enumeration procedures, which involve adding the Colilert-18 reagent to a water sample, incubating, and reading results to determine if bacteria are present or quantify the MPN.

Uploaded by

Sreedhar Reddy Sajjala
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

MOST PROBABLE NUMBER (MPN)

Introduction
Colilert*-18 either simultaneously detects total coliforms and E. coli; or
fecal coliforms in water. It is based on IDEXXs patented Defined
Substrate Technology* (DST*). When total or fecal coliforms metabolize
Colilert-18s nutrient-indicator, ONPG, the sample turns yellow. When E.
coli metabolize Colilert-18s nutrient-indicator, MUG, the sample also
fluoresces. Colilert-18 can simultaneously detect these bacteria at 1
cfu/100 mL within 18 hours even with as many as 2 million
heterotrophic bacteria per 100 mL present.
Presence/Absence (P/A) Procedure
1. Add contents of one pack to a 100 mL sample in a sterile,
transparent, nonfluorescing vessel. 2. Cap vessel and shake.
3. If sample is not already at 3338C, then place vessel in a 35C
waterbath for 20 minutes or, alternatively, a 44.5C waterbath for 710
minutes.
4. Incubate at 350.5C for the remainder of the 18 hours. 5. Read
results according to Result Interpretation table below.
Quanti-Tray* Enumeration Procedure
1. Add contents of one pack to a 100 mL room temperature water
sample in a sterile vessel.
2. Cap vessel and shake until dissolved.
3. Pour sample/reagent mixture into a Quanti-Tray* or QuantiTray*/2000 and seal in an IDEXX Quanti-Tray* Sealer.
4. Place the sealed tray in a 350.5C (or 44.50.2C for fecal
coliforms) incubator for 18 hours (prewarming to 35C is not required).
For incubation in a water bath, submerge the Quanti-Tray, as is, below
the water level using a weighted ring.
5. Count the number of positive wells and refer to the MPN table
provided with the trays to obtain a Most Probable Number.

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