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12 Nationaland3 International
ConferenceofBotany

ABSTRACTBOOK

Septemebr1stto3rd2012

QuaideAzamUniversity,
ISLAMABAD,PAKISTAN

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Contents

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Theme 1: Agriculture Agronomy, Horticulture, Plant Breeding & Genetics

Oral Presentations
OPBG 1
OPBG 2
OPBG 3
OPBG 4
OPBG 5
OPBG 6
OPBG 7
OPBG 8
OPBG 9
OPBG 10
OPBG 11
OPBG 12
OPBG 13
OPBG 14
OPBG 15
OPBG 16
OPBG 17
OPBG 18
OPBG 19
OPBG 20

Abdul Kabir Khan Achakzai and Habibullah - Effect of nitrogen fertilizer on the yield of
Mungbean cultivars under the agroclimatic conditions of Quetta
Akbar Ali Bhatti, Adnan Younis, Atif Riaz, Bilquees Fatima, Mansoor Hameed, Usman
Tariq - Callogenesis and embryogenesis in orchid (Dendrobium sabin H.) under different levels
of growth regulators
Armghan Shahzad, Sami Ashraf, Farwa Karamat, Muhammad Iqbal, Jalal-Ud-Din and
Ghulam Muhammad Ali - Quantitative trait loci mapping of drought tolerance at germinaiton
stage in bread wheat
Farah Naz, C.A. Rauf, I. Ahmad, I.U. Haque and A. Riaz - Management of black scurf of
potato with effective microbes, biological potassium fertilizer (bpf), and Trichoderma harzianum
Ghulam Mustafa, Muhammad Maqsood Alam, Sami Ullah Khan, Muhammad Naveed and
Abdul S. Mumtaz Leaf rust resistance in semidwarf wheat cultivars: a conspectus of post green
revolution period in Pakistan
Ghulam Sarwar Markhand, Adel Ahmed, Abul-Soadand Mushtaque Ahmed Jatoi - Field
evaluation of three Saudi Arabian date palm varieties (Ajwa, Safawi and Ruthana) at Khairpur,
Pakistan
Hamd Meer, Shazia iram, Iftikhar Ahmad, Faisal Sohail Fateh, Munawar Raza Kazmi Identification and characterization of post harvest fungal pathogens of mango from domestic
markets of Punjab
Hidayatullah, Asghari Bano and Khalid Mahmud Khokhar - Effect of pruning on sex
expression and its relation with phytohormones content in monoecious cucumber
Hidayat ur Rahman, Asif Ali, Zahir Shah, M. Iqbal and M. Noor - Line tester analysis for
grain yield and yield related traits in maize variety Sarhad white
Ihsan Ullah, Syeda Maariyah Hasni, Muhammad Zaffar Iqbal, Muhammad Nawaz and
Shakra Jamil - Molecular characterization of olive germplasm using DNA markers
Israr Asghar, Muhammad Akmal, Muhammad Ishtiaq Ch, Mehwish Maqbool, Khalid
Saifullah Khan, Ghulam Shabbir - Analysis of soil microbial biomass dynamics in rainfed
wheat fields in arid zone of Pakistan
Khalid Mehmood, Muhammad Arshad, Ghulam Muhammad Ali, Rahmatullah Qureshi
And Abdul Razzaq - Tissue culture responses of some wheat (triticum aestivum L.) cultivars
grown in Pakistan
Madeeha Khalid, Tariq Mahmood, Awais Rasheed, Glvina Gul Kazi, Abdul Mujeeb-Kazi Glu-dt1 allelic variation in synthetic hexaploid wheats derived from durum cultivar decoy
Aegilops tauschii accessional crosses
Makhdoom Hussain, Mubashir niaz, Muhammad Iqbal, Javed Ahmad, Muhammad Asif,
G.M. Subhani, Sajid-ur-Rahman and Tehreema Iftikhar Effect of pollen sources and ploidy
levels on doubled haploid production in wheat
Maqsood Ahmed, Hafiz Ashfaq Ahmed, Rahmatullah Qureshi, Nadra Khan, R. Mohib
Muazzam, M. Riaz Khan Developing protocol for genetic transformation of grapes (Vitis
vinefera) King, s Ruby C.V
Muhammad Ali Khan, Noor Ul Amin, Muhammad Sajid, Asif Shah and Muhammad
Rezaul Kabir Therapeutic horticulture: influencing psychological responses of surgical patients
and their environmental assessment scale
Muhammad Asad Mukhtar, Muhammad Ansar, Shahzada Sohail Ijaz Muhammad Azeem
Malik and Ghulam Shabbir - Forage yield as affected by common vetch in different seeding
ratios with winter cereals in pothowar region of Pakistan
Muhammad Fayyaz, Atiq-ur-Rehman Rattu, Shahzad Asad, Muhammad Iqbal, Khalil
Ahmed Khanzada, Muhammad Zakria, Javed Iqbal Mirza and Shamim Iftikhar
Virulence pattern of stripe rust (Puccinia striiformis) in Pakistan
Muhammad Iqbal, Mahwish Ejaz, Mahrukh Bibi, Iftikhar Ahmed, Armghan Shahzad and
Ghulam Muhammad Ali Molecular genetic variation for stripe rust resistance in Pakistani
spring wheat
M. Irfaq, Mir Ajab Khan, Gul Sanat Shah Khattak, Abdul Jabbar Khan, Tila Mohammad
and Fazle Subhan - Number of genes and their effects controlling grain filling duration in two
wheat (Triticum aestivum L.) crosses

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OPBG 21 M.I. Haque and Syed Zia-ul-Hussnain - Acidovorax avenae subsp. avenae first time causing
red stripe of sugarcane in Pakistan
OPBG 20 Muhammad Javad Seghatoleslami, Gholamreza Mousavi and Mahdi Jafari - The effect of
planting date, irrigation and nitrogen on some traits of forage millet (Pennisetum americanum var.
Nutrifeed)
OPBG 23 M. Ramzan Anser, Faisal Zahoor, Muhammad Azim Malik, Khalid Mahmood, Mushtaq
Hussain Kazmi, Muhammad Raheed and S.H. Raza - Wheat response to various tillageherbicide interactive systems under semi-arid climate
OPBG 24 M. Yasin Ashraf, M. Ashraf, M. Akhter and Javed Akhter - Improvement in fruit yield,
quality and fruit droping control in kinnow (Citrus reticulata Blanco) through application of
growth regulators, potassium and zinc
OPBG 25 Nizamuddin Depar, Inayatullah Rajpar, Muhammad Yousuf Memon, Javaid Ahmed Shah
and Muhammad Afzal Arain - Tailoring some coarse and fine rice genotypes for low-zinc-input
sustainable agriculture
OPBG 26 Sadar Uddin Siddiqui, Toshihiro Kumamaru and Hikaru Satoh - Variation and distribution
in seed storage starch amylose content and its associated 60kd waxy protein band in Pakistan rice
genetic resources
OPBG 27 Sajjad Hussain, Muhammad Akbar Anjum, Franck Curk, Franois Luro, Gilles Tison Performance evaluation of common clementine on various rootstocks
OPBG 28 Sajid-ur-Rahman and Tanwir Ahmad Malik - Tagging genes for velvet hairiness in cotton
using RAPD markers and bulked segregant analysis
OPBG 29 Sana Liaqat, Madiha Sadiq, Kousain Kousar, Muhammad Fayyaz, Farrakh Mehboob,
Robina Khan, Sumaira Farrakh - Assessment of genetic diversity in stripe rust resistant
NUWYT lines using microsatellite markers
OPBG 30 Shafqat Farooq, Hafeez Sadaqat and Farooq e Azam - Screening and stability analysis of salt
tolerance wheat genotypes under fields of higher salinities
OPBG 31 Shahid Masood Shah, Ghulam Shabir, Muhammad Sabar, Kashif Aslam, Javed Iqbal
Wattoo, Shahzad Aamir Naveed, Nourin Ashiq, Raheela Waheed, and Muhammad Arif Genetic diversity in basmati and non-basmati rice varieties based on microsatellite markers
OPBG 32 Shahid Nazir and Muhammad Sarwar Khan - Integration of novel chlorophyll genes from
black pine into the chloroplast genome of tobacco
OPBG 33 Shehla Shinwari, Abdul Samad Mumtaz, M. Ashiq rabbani, Fazal Akbar and Zabta Khan
Shinwari - Genetic divergence in taramira (Eruca sativa L.) germplasm based on quantitative and
qualitative characters
OPBG 34 Seyyed Gholamreza Moosavi, Mohamad Javad Seghatoleslami, Amir Ebrahimi and
Zeinolabedin Jouyban - The effect of nitrogen rate and plant density on morphological traits and
essential oil yield of coriander
OPBG 35 Syed Mubashar Sabir, Syed Rizwan Abbass, Syed Asad Hussain Shah, Syed Dilnawaz
Ahmad - Antioxidant activitxy and protecting ability of different cultivars of sugarcane against
DNA damage
OPBG 36 Wajid Nasim, Muhammad Farroq Hussain Munis, Hassan Javed Chaudhary, Muhammad
Shahid - Climate change impact on sunflower productivity under agro-environmental conditions
of Pakistan: Simulation & field study
OPBG 37 Z.A. Deho, S. Laghari, S. Abro, M.A. Arain, S.D. Khanzada and Fakhruddin - Agronomic
evaluation of cotton (Gossipium hirsurum L.) advanced strains for fiber quality and yield
parameters under Tando jam environmental conditions

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Poster Presentations
PPBG 1
PPBG 2
PPBG 3
PPBG 4

Abdul Sattar, Mumtaz A. Cheema, M.A. Wahid, M.F. Saleem, H. Munir, Fahd Rasul and
N. Sarwar - Phenology and accumulative heat unit of various wheat cultivars under late sowing
high temperature
Abida Kausar, M. Yasin Ashraf, Mubashir Niaz, M. Ashraf and Qaiser Abbas - Some
physiological and genetical determinents of salt tolerance in sorghum (Sorghum bicolor L.):
Biomass production and salinity mediated nitrogen metabolism
Aftab Wajid, Ashfaq Ahmad, Gerrit Hoogenboom, Tasneem Khaliq and M. Usman Modeling growth, development and seedcotton yield of promosing cotton cultivars at varying
nitrogen increments with different planting dates under DSSAT
Allah Wasaya, Fayyaz-ul-Hassan, Muhammad Tahir , Muhammad Ansar and Abdul
Manaf - Physiological expressions and dry matter production of maize (Zea mays L.) in response
to tillage and nitrogen application

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PPBG 5
PPBG 6
PPBG 7
PPBG 8
PPBG 9
PPBG 10
PPBG 11
PPBG 12
PPBG 13
PPBG 14

PPBG 15
PPBG 16
PPBG 17
PPBG 18
PPBG 19
PPBG 20
PPBG 21
PPBG 22
PPBG 23
PPBG 24
PPBG 25
PPBG 26

PPBG 27

PPBG 28

Ammad Abbas, Yusuf Zafar, Muhammad Atif, Andrew H. Paterson Mehboob-ur-Rahman

- Preliminary studies of association mapping in cotton
Ammara Rauf, Farooq A. Khan, Muhammad Aslam and Muhammad Nadeem Anwar Assessment of salt tolerance among different sunflower accessions
Amjad Ur Rahman and Abdul Samad Mumtaz - Evaluation of genetic diversity in bread
wheat (Triticum aestivum L.) by SDS-page analysis
Arshad Javaid and Saima Rauf - Bioassays guided fractionation of Chenopodium album L., for
evaluation of its antifungal activity to control onion basal rot pathogen Fusarium oxysporum f. sp. Cepae
Asif Javaid, Abdul Ghafoor and M. Shahid Masood - Exploring the genetic potential of
Plantago ovata germplasm
Asma Hassan, Shahzada Sohail Ijaz, Safdar Ali, Muhammad Ansar, Khalid Saifullah Khan
and Qaiser Hussain - Tillage and crop sequence effect on soil organic carbon fractions and
aggregate stability in dryland Pothwar, Pakistan
Asma Safdar, Sajid Aleem Khan and M.A. Safdar - Incidence of Botryodiplodia theobromae
on guava orchards in district sheikhupura its chemical management
Aurangzeb Rao, Syed Dilnawaz Ahmad, Syed Mubashar Sabir, Shahid Awan, Asad
Hussain Shah, Syed Rizwan Abbas, Saima Shafique, Sardar Ali and Fareed Khan Antioxidant activity and lipid peroxidation of selected wheat cultivars under salt stress
Aysesha Tania, Abida Akram, Nafeesa Qudsia Hanif, Muhammad Arshad and Abdul Rauf
- Nutritional profile, mycoflora assessmesnt and afaltoxin contamination in chickpea (Cicer
arietinum L.)
Azra Yasmeen, Shahzad Maqsood Ahmed Basra, Muhammad Mansoor Javaid, Hafeez-urRehman, Abdul Wahid and Nazim Hussain - Effect of exogenous application of natural and
synthetic growth enhancers on quantitative and qualitative attributes of tomato (Lycopersicum
esculentum)
Erum Mukhtar, Ejaz Hussain Siddiqi, Khizar Hayat Bhatti, Khalid Nawaz and Khalid
Hussain - Gas exchange attributes can be valuable selection criteria for salinity tolerance in
canola cultivars (Brassica napus L.)
Faisal Hafeez, Waseem Akram, Unsar Naeem-Ullah, Khuram Zia and Hafiz Azhar Ali
Khan - Boicidal properties of citrus oils against dengue mosquito Aedes albopictus
Faiza Mushtaq, Shakeel A. Jatoi, Shah Jehan Baloch, Waseem Hassan Shah and Sadar
Uddin Siddiqui - Diversity analysis in chilies for agronomic traits and total seed protein profile
Fakhra Ambreen, Noshin Ilyas, Qudsia Bano, Rehmatullah Qureshi, Muhammad Arshad,
Naveed Iqbal Raja - Beneficial effect of Vigna radiata L., to subsequent wheat crop
Farah Naz, C.A. Rauf and I.U. Haque and G. Irshad - Inhibition of Rhizoctonia solani with
isothiocyanates produced by brassicaceae species
G. Sarwar, M. Hassan and A. Hussain - Genotypic environment interaction of castorbean
grown over multiple years
G.S. Channa, A.R. Mahar, I. Rajpar, Zia-ul-hassan, J.H. Umrani and W.A. Maitlo - Comparing
salinity tolerance of five high yielding, non-aromatic rice (Oryza sativa L.) cultivars of Sindh
Ihsan Ullah, Shahid Nazir, Muhammad Zaffar Iqbal and Anam Shahbaz - Molecular
characterization of linseed germplasm using DNA markers
Khalil Ahmed Laghari and Mahboob Ali Sial - Wheat germplasm characterization on the basis
of morphological markers
Mahboob Ali Sial, Muhammad Ahmed Arain, Amanullah Baloch, Khalil Ahmed Laghari
and Shafi Muhammad Jariko - Genetic analysis for some quantitative traits in F4 segregating
population of wheat
Malka Saba and Abdul Nasir Khalid - Species diversity of genus Puccinia Pers.
(Basidiomycota; Uredinales) parasitizing poaceous hosts from Pakistan
Maqsood Ahmed, Syed Mubashar Sabir, Qaisar Shabbir M. Riaz Khan, R. Mohib Muazzam
and Abid Yaqoob - Physico-chemical characteristics, phenolic content and antioxidant activities of
apple (Malus domestica Borkh) varities/genotypes from Azad Jammu and Kashmir
Mubashir Hussain, Muhammad Ansar, Safdar Ali, Ahmad Sher and Hafiz Muhammad
Jhanzab - Physiological and forage performance of hybrid vs composite Brassica varieties under
rainfed conditions
Muhammad Adnan, Asif Ahmad, Anwaar Ahmed, Nauman Khalid, Imran Hayat and
Iftikhar Ahmed - Chemical composition and sensory evaluation of tea (Camellia sinensis)
Commercialized in Pakistan

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PPBG 29 Muhammad Akhtar, Asif Naeem, Faqir Hussain, M. Yasin Ashraf, Javed Akhter and K.
Mahmood - Enhancing phosphorus use efficiency in cereals by phosphoric acid application in
alkaline calcareous soils
PPBG 30 Muhammad Ali, Mukhtar Ahmad, Naveed Anjam, Hafeez-ur-Rehman and Sudheer Tariq Effect of Boron and GA3 on the flowering and fruit setting in Olive (Oleaeuropeae L.) cv. uslu.
PPBG 31 Muhammad Ali Khan, Muhammad Atiqullah Khan and Shamim-ul-Sibtain Shah - Effects
of phosphorus levels alone or in combination with farmyard manure on growth, yield and nutrient
contents of wheat in rainfed conditions
PPBG 32 Muhammad Arif, Muhammad Azim Malik, M. Ramzan Anser, Zammurad Iqbal Ahmed
and M. Ansar - Spatio-temporal cropping systems to enhance harvest of residual soil moisture
content for succeeding crops in Pothwar plateau
PPBG 33 Muhammad Arshad, M. Ayub Khan and Muhammad Amjad - Performance of locally
developed sunflower hybrids in Pakistan
PPBG 34 Muhammad Asghar, Afsari S. Qureshi, Fayaz Ahmad Chaudhry, Asghar Ali, Mukhtar
Ahmad, Abdul Qadir, Asghar Abbas, Mhammad Faisal Anwar Malik and Zafeer Saqib Effects of gamma () irradiation on hydrocyanic acid contents in Sudan grass (Sorghum vulgare
var, Sudanese)
PPBG 35 Muhammad Aslam, Amer Maqbool, Qamar uz Zaman and Haris Bilal - Physiological and
biochemical response of cotton genotypes to different salinity levels at early growth stages
PPBG 36 Muhammad Awais, Aftab Wajid, Ashfaq Ahmad, Tasneem Khaliq, M. Farrukh Saleem,
Usman Bashir and M. Habib ur Rehman - Growth, yield and radiation use efficiency of maize
hybrids at varying nitrogen levels
PPBG 37 Muhammad Haroon, Muhammad Saeed, Mudassar Iqbal, Hamida Bibi and Jehanghir
Khan - Developing a sustainable and eco-friendly weed management in maize
PPBG 38 M. Hassan, A. Rashidand I. Cakmak - Improvement in flag-leaf area of wheat by zinc
application: its effect on yield and grain-zinc concentration
PPBG 39 M. Iftikhar, S. Akhter, A. Rehman, T.A. Khattak, K. Kaleem, M. Shoaib, Z.R. Khalil, and
M. I. Khattak - Effects of Aniseed (Pimpinella anisum) on overall performance and milk
composition of Damani goats
PPBG 40 Muhammad Ijaz, Fayyaz-ul-Hassan, M.I. Haque, C.A. Rauf and M. Tariq - Influence of crop
rotation and sowing time on epidemic of cercospora leaf spot of peanut under rainfed conditions
PPBG 41 Muhammad Ilyas Khokhar, Jaime A. Teixeira da Silva, M. Zaffar Iqbal, Saleem Akhtar
and Mubashir Niaz - Evaluation of barley genotypes for yielding ability and drought tolerance
under irrigated and water-stressed conditions
PPBG 42 Muhammad Ishtiaq, Tanveer Hussain and Mehvish Maqbool - Rapid declining of mango,
citrus and sheesham trees: An environmental issue & its consequences on socioeconomics of
bhimber area (AK) Pakistan
PPBG 43 Muhammad Kashif Shahzad Sarwar, Mehboob-ur-Rahman, M. Yasin Ashraf Muhammad
Zaman and Yusuf Zafar - Genetic variability in proline and its relationship with yield and yield
parameters of cotton cultivars grown under water stress conditions
PPBG 44 Muhammad Mazhar Hussain, Asif Saeed, Asif Ali Khan, Amir Shakeel, Nadeem Saeed and
M. Massub Tehseen - Analysis of combining ability status among parents and hybrids in Tomato
PPBG 45 Muhammad Rizwan, K. Mahmood, M.Y. Ashraf, M.I. Chughtai, A.R. Awan and M. Saleem
- Screening of forage genotypes for salt tolerance and forage production in saline environments in
Pakistan
PPBG 46 Muhammad Tasdiq Hussain Shahid, Farooq Ahmad Khan, F.M. Azhar, Bilquees Fatima
and Muhammad Aslam - Variability assessed in red rot resistant somaclones of sugarcane
genotype S97US297 in R1 and R2 generations
PPBG 47 Muhammad Yaseen, Muhammad Arshad and Wazir Ahmed - Effect of foliar feeding on
biofortification of wheat
PPBG 48 Mushtaque Ahmed Jatoi - Date palm cultivation in sindh: current status and future prospects
PPBG 49 Nadia Mubarik, Aqib Iqbal, Ijaz Ali and Zahoor Ahmad Swati - Chemicals application to
alleviate drought stress and enhance productivity of Brassica napus cv Bulbul-98 under limited
moisture conditions
PPBG 50 Naeem Sarwar, Muhammad Maqsood, Muhammad Ashfaq, Ehsan Ullah, Hakoomat Ali,
Tasneem Khaliq, Khuram Mubeen and Abdul Sattar - Response of fine rice (Oryza sativa L.)
to application of various micronutrients in different rice cultures
PPBG 51 Nafisa and Amna Shoaib - Expression of defensin like gene in Pisum sativum

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PPBG 52 Naseem Sharif, Noor-un-Nisa Memon, Fakhruldin Razi and Muhammad Jafar Jaskani Categorization of ber germplasm through bearing and associated Characters
PPBG 53 Nausherwan Nobel Nawab, Abid Hussain, Ghulam Jeelani, Muhammad Sudheer Tariq, Taj
Naseeb Khan, Muhammad Farooq and Ali Raza Gurmani - Inheritance of okra leaf type,
gossypol glandsand trichomes in cotton
PPBG 54 Nazim Hussain, Muhammad Bismillah Khan, Riaz Ahmad and Niaz Ahmed - Agrophysiological response of some wheat cultivars to phosphorus
PPBG 55 Niaz Ahmed, Muhammad Arif Ali,Muhammad Abid, Fiaz Ahmad and Nazim Hussain Effect of zinc fertilization on dry matter production and mineral composition of irrigated cotton
PPBG 56 Omer H. Malik, Aman U. Malik, Habat U. Asad, Muhammad Amin, Syed A. Raza and
Muhammad S. Khalid - Mango fruit quality, storability and marketability in response to
precooling and various fungicidal treatments
PPBG 57 Qurat-ul-Ain Bughio, Pervish Sial, Mahboob Ali Sial and Khalil Ahmed Laghari - Effects of
gamma irradiation on seedling stage of different wheat varieties
PPBG 58 S. Ahmad, H. Ali, S.U. Khan, U. Farooq, M. Zia-ul-Haq and M.A. Khan - Nitrogen and
radiation use efficiencies of C4 summer cereals to nitrogen split application
PPBG 59 S. Ahmed, N. Zaman and S. A. Khan - Effect of intercropping on root rot disease and
agronomic performance of groundnut
PPBG 60 Saiema Rasool, T.O. Siddiqi and Parvaiz Ahmad - Genetic diversity as revealed by RAPD
analysis among the chickpea genotypes
PPBG 61 Saleem Akhtar , Mubashir Niaz , Muhammad Younas, Sajid ur Rehman , Muhammad
Zaffar Iqbal and Tehreema Iftikhar - Comparison of wheat somaclones with their respective
parents under salt stress conditions
PPBG 62 Sanam Asif, Abida Akram, Nafeesa Qudsia Hanif, Rahmatullah Qureshi And Abid Riaz Assessment of seed-borne mycoflora and nutritional profile of lentil (Lens culinaris) grown in
arid region
PPBG 63 Sana Ullah, Fahd Rasul, A. Sattar, Mumtaz A. Cheema, M.F. Saleem, M.A. Wahid and A.
Ghaffar - Zinc application responses of autumn planted sunflower hybrids on phenological, yield
and quality attributes
PPBG 64 Shah Fahad, Amjad ur Rahman, Zia-ur-Rehman Mashwani, Abdul Samad Mumtaz and
Asghari Bano - Efficacy of different herbicides for weed management in wheat crop
PPBG 65 Shahid J. Butt - Production and quality improvement of lettuce (Lactuca sativa L.) through
containerized growing media under cold glasshouse conditions
PPBG 66 Shakeel A. Jatoi and Kazuo N. Watanabe - Diversity analysis and relationships among ginger
landraces
PPBG 67 Shayan Syed, Mahboob Ali Sial and Khalil Ahmed Laghari - Comparative performance of
semi-dwarf and dwarf wheat genotypes
PPBG 68 Shazia Shahzaman, Farah Naz, C.A. Rauf, I.U. Haque and K.Riaz - Pathogenic Variations
and characterization of Streptomyces scabies isolates from potato tubers in Rawalpindi district
PPBG 69 Sheikh Muhammad Iqbal, Abdul Ghafoor and Iftikhar Ahmad - Investigations for
complexity of Ascochyta blight of chickpea and identification of resistant sources
PPBG 70 Shumaila Bano, Ghulam Mustafa, Sana Bashir, SamiUllah Khan, Muhammad Naveed and
Abdul Samad Mumtaz - Probing genetic resistance against stem rust in wheat varieties of
Pakistan
PPBG 71 Shujaat Ali, Azhar Hussain Shah Rahmani Gul, Safdar Hussain Shah, Habib Ahmad and
Zafar Iqbal - Morpho-agronomic characterization of okra germplasm
PPBG 72 Sohail Akhter, Farooq Ahmed Khan, Muhammad Aslam Javed and Muhammad Ashfaq
Anjum - Assessment of genetic diversity in some accessions of Saccharum officinarum L., by
means of morphological characters, with Principal Component Analysis (PCA)
PPBG 73 Sohail Akhter, Farooq Ahmed Khan, Muhammad Aslam Javed and Muhammad Ashfaq
Anjum - Microsatellite (SSR) markers a tool; for genetic diversity assessment among sugarcane
accessions
PPBG 74 Sonia George, Shakeel A. Jatoi, Nasir M. Minhas and Sadar Uddin Siddiqui - Genotypic
differences against PEG simulated drought stress in Tomato
PPBG 75 Sumera Anwar, M. Yasin Ashraf, Mumtaz Hussain and Muhammad Ashraf - Cadmium
induced changes on growth, yield and element uptake of maize and its phytoextraction through
EDTA and citric acid
PPBG 76 Syed Mehar Ali Shah and Muhammad Riaz - Genetic diversity in indigenous rice germplasm
for qualitative and quantitative traits

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PPBG 77 Syed Muhammad Akmal Rahim, Farkhanda Jabeen and Syed Muhammad Ajmal Rahim A technique for land management alternatives based on identification of appropriate tree species
corresponding to particular soil attributes of district vehari, (Punjab), and Pakistan
PPBG 78 Tasneem Khaliq, Ashfaq Ahmed, Ghulam Mustafa Tahir, Jamshad Hussain and M. Imran
Akram - Impact of different planting patterns on growth, yield and radiation use efficiency of
spring maize (Zea mays L.) in semiarid environment
PPBG 79 Waqas Hussain, Zahid Akram, Talat Mehmood, Ghulam Shabbir, Rehmatullah Qureshi
and Abdul Mujeeb Kazi - Evaluation of molecular mapping population from wheat/synthetic
hexaploid crosses for drought tolerance
PPBG 80 Wazir Ahmed, Muhammad Yaseen, Muhammad Arshad and Muhammad Shahid - Salicylic
acid pre-soaking for germination of sweet pepper under salt stress
PPBG 81 Usman Tariq, Adnan Younis, Shoaib Ur Rehman, Atif Riaz and Mansoor Hameed Evaluation of rice hull as potting substrate for growth and flowering of Dahlia hortensis
PPBG 82 Zahid Hussain, Fazal Munsif, Khan Bahadar Marwat, Riaz Ahmad Afridi and Shahida Bibi
- Evaluation of various herbicides for weed management in potato in Peshawar Pakistan

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Theme 2: Biotechnology / Biochemistry / Bioinformatics

Oral Presentations
OPBB-1

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OPBB-3
OPBB-4
OPBB-5
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OPBB-7
OPBB-8
OPBB-9
OPBB-10
OPBB-11
OPBB-12
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OPBB-14
OPBB-15

Ahmad Ali, Muhammad Arshad, Anna Maria Mastrangelo, Pasquale De Vita, Awais
Rasheed, Alvina Gul-Kazi, Tariq Mahmood and Abdul Mujeeb-Kazi - Comparative
assessment of glutenin composition and their relationship with grain quality traits in bread wheat
germplasm
Armghan Shahzad, Sami Ashraf, Farwa Karamat, Muhammad Iqbal, Jalal-ud-Din and
Ghulam Muhammad - Quantitative trait loci mapping of drought tolerance at germinaiton stage
in bread wheat
Bushra Hafeez Kiani, and Bushra Mirza Comparative Artemisinin analysis in Artemisia annua
and Artemisia dubia transformed with two different Agrobacteria harbouring rol ABC gene
Fariha Khan, Tasawar Sultana, Farah Deeba and S. M. Saqlan -Dynamics Of Mrna Of
Glycine-Rich Rna-Binding Protein During Wounding, Cold And Salt Stresses In Nicotiana
Tabacum
Holger Densow, Abdul Razaque Memon, Elif Yuzbasioglu, Matthias Fricke, Markus Sauer
and Karsten Niehaus - Molecular cloning and characterization of ARF1 and COPI coat proteins
from Medicago truncatula cv. Jemalong
Iram Liaqat and Shahid Khan - Bacaterial biofilm formation inhibition by blocking flagellar
assembly
Idris Arslan, Ali Celik, Kristina Jenett-Siems and Matthias F. Melzig - In vitro cytotoxic
enhancing activity of triterpen saponins from Gypsophila pilulifera on saporin a type-I RIPs
Imran Mahmood, Abdul Razzaq, Zaheer-ud-Din Khan, Ishfaq Ahmad Hafiz and Shuaib
Kaleem - Evaluation of tissue culture responses of promising wheat (Triticum aestivum L.)
cultivars and development of efficient regeneration system
Kanwal Batool, Sheeraz Ahmed, Chaudhary Abdul Rauf and S.M. Saqlan - Amplification
and sequencing of internal transcribed regions 1& 2, and 5.8S rDNA from local isolates of
Fusarium species
Khizar Hayat Bhatti, Aminullah Shah, Qaisar Mehmood, Khalid Nawaz, Khalid Hussain,
Ejaz Hussain Siddiqi, Wu Jiahe and Zhu Chuanfeng - Transgenic tobacco with rice fae gene
shows enhanced resistance to drought stress
Mariam Rukhama Farman, Maria Saleem, Saadia Naseem and Fauzia Yusuf Hafeez Mining of polymorphic microsatellite markers In Silico for diagnosis of basmati rice adulteration
Maryum Zeb, Muhammad Kausar Nawaz and Rani Faryal - Development of putative
molecular markers to trace durable rust resistance genes in wheat breeding stocks
Muhammad Azeem Asad, Yan Ren, Xianchun Xia, Chengshe Wang, Zhonghu He Molecular mapping of stripe rust resistance gene Yrsn 78 in wheat line Shaannong 78
M. Farooq Hussain Munis, Asghari Bano, Hassan Javed Chaudhary, Muhammadi Optimization of quantitative real-time PCR analysis for reliable detection and quantification of
Fusarium oxysporum in wheat
Muhammad Ishtiaq Ch, Abdul Samad Mumtaz, Mehwish Maqbool, Yi Wang - Leaf
Proteome Analysis of Clematis chinensis: a Traditional Chinese Medicine (TCM) by Twodimensional Electrophoresis Technique

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OPBB-16 Muhammad Younas Khan Barozai - Profiling the Carrot (Daucus carota) MicroRNAs and their
Targets
OPBB-17 Munazza Ihtisham, Ihsan-ul-Haq, Sara Sarwar, Samreen Saleem, Laila Jafri, Nazif Ullah and
Bushra Mirza - HPLC-DAD analysis and free radical scavenging potential of quercus dilatata
OPBB-18 Neelam Taj, Muhammad Zia, Yamin Bibi, Naveed Iqbal and M. Fayyaz - Factors affecting
Agrobacterium tumefaciens mediated genetic transformation of Soybean cultivar NARC-7
OPBB-19 Nabila Tabbasam, Yusuf Zafar, Mehboob-ur-Rahman and Andrew H Paterson - BAC
derived new SSRs for use in cotton (Gossypium spp.) improvement
OPBB-20 Raheela Rehman, Kausar Nawaz Shah, M. Shahid Masood, Muhammad Arshad, M. Fahim
Abbas and Abdul Ghafoor - Genetic divergence among Pakistani bread wheat varieties and
advanced lines for randomly amplified polymorphic dna (RAPD) markers
OPBB-21 Rehan Naeem and Bushra Mirza - Development and characterization of barley core collection:
A strategy for germplasm management
OPBB-22 Riaz-ur-Rehman, Gaung Lu,Abdul Mannan, Muhammad Fayyaz Chaudhary, Muhammad
Zia - Evaluation of Caralluma tuberculata plant and callus extracts as free radical scavenger
OPBB-23 Sadia Banaras, Sitwat Aman, Maryam Zafar, Mudassar Khan, Safdar Abbas, Zabta Khan
Shinwari, Samina N Shakeel-Molecular identification and comparative analysis of novel 18S
ribosomal RNA genomic sequences of wide range of wild medicinal plants
OPBB-24 Samina N. Shakeel, Sitwat Aman, Noor-ul-Haq and Scott A. Heckathorn Dawn Luthe Proteomic and transcriptomic approach to explore the molecular basis of adaptation of agave
americana to heat stress
OPBB-25 Shahid Nazir and Muhammad Sarwar Khan - Integration of novel chlorophyll genes from
black pine into the chloroplast genome of tobacco
OPBB-26 Shahzadi Faiza Safdar and Hamid Rashid - Alternate resistance gene against yellow rust in
wheat
OPBB-27 Siffatullah Khan, Shama Naz, Reema and Rehan Naeem - Genetic fingerprinting of local
turmeric genotypes using rapds
OPBB-28 Sarwat Jahan And Mehreen Khan - Comparative analysis of antioxidants against cadmium
induced reproductive toxicity in adult male rats
OPBB-29 S. Rani, S. Niaz , U. Younis, S. A. Malik, Z. Mahmood and R. Raja - Analysis of Pakistani
rice genome polymorphism by using RAPD PCR
OPBB-30 Tahsin Gulzar, Sadaf Ambreen, Muhammad Younas and Zafar Iqbal - Assessment of
genetic relationships among wheat genotypes by rust resistant markers and bioinformatics tools
OPBB-31 Zahid Ali, Hans Martin Schumacher, Heiko Kiesecker and Hans Joerg Jacobsen - Selection
of Transgenic Cells/Explants using Na+/H+ antiporter gene as a selectable marker A sustainable
approach

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Poster Presentations
PPBB 1

Abbas S.R, S.D. Ahmad, S.M. Sabir, A.H. Shah, S.I. Awan and A.Majeed - Comparative lipid
peroxidation, leaf membrane thermostability, and antioxidant system in thirteen sugarcane
genotypes on differing PEG concentrations
PPBB 2 A.A. Abul-soad, G. S. Markhand and M.A. Lakho - In vitro rooting and acclimatization of
pineapple (Ananas comosus L. Merr.)
PPBB 3 Abdul Ghafoor - Exploitation of genetic diversity for development of powdery mildew resistant
and lodging tolerant genotypes of Pisum sativum throughmarker assisted selection
PPBB 4 Abdul Razaque Memon, Lamija Subasic, Birsen C Keskin and Yasemin Yildizha Genomics and transcriptomics analysis of metal accumulator plants in Brassicaceae
PPBB 5 Alveena Tariq, Safdar Ali And Zaheer-ud-Din-Khan - Detection and appraisal of phellandrene in callus culture of Momordica charantia L. cv. Jaunpuri and Jhalri
PPBB 6 Amjad Hameed, Madiha Goher and Nayyer Iqbal - Biochemical indices of drought tolerance
in wheat at sarly seedling stage
PPBB 7 Amna Bashir, Tariq Mahmood, Beenish Saba Raja and Mian Nazish Adnan - Spatiotemporal monitoring of ground level ozone in twin cities of Pakistan
PPBB 8 Azhar Mehmood, Muhammad Sajid, Habib Ahmad and Azhar Hussain Shah - Effect of
Different Concentrations Of Ga3 on in Vitro Micropropagation of Potato Varieties
PPBB 9 Bushra Huma, Tanzila Sahar, Sobia Aleem, Humara Naz Majeed and Munir Ahmad
Sheikh - Effect of green tea on the production of advanced glycation end products
PPBB 10 Darima Habib, Muhammad Zia, Yamin Bibi, Muhammad Fayyaz Chaudhary - Antioxidant
enzymes activities during regeneration of Argyrolobium roseum through callogenesis and
organogenesis

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PPBB 11 Ghazala Nasim and H. M. Waqas - Mycographic analysis of macromycetes of ayubia national
park, development of identification software and indication of threatened species
PPBB 12 Habib Ahmad and Shahida Hasnain - Elaborating the role of genome B and C for suppressing
homoeologous pairing in genome A of brassica
PPBB 13 Israr Ahmad, Habib Ahmad, SajidulGhafoor and Ajmal Iqbal - Reassessment of Mentha
species from Kunhar river catchment
PPBB 14 Jharna Rafaqat, Farooq Latif, Muhammad Hamid Rashid, Tehreema Iftikhar and
Mubashir Niaz - Avicelase (exoglucanase) productivity of Humicola insolens FLN-1 grown
under submerged conditions on various carbon sources
PPBB 15 Jharna Rafaqat, Farooq Latif, Hamid Rashid, Tehreema Iftikhar and Mubashir Niaz Production, partial purification and characterization of avicelase (exocellulase) from Humicola
insolens FLN-1
PPBB 16 Kaleem Ullah Kakar, Bilal Haider Abbasi, Zarqa Nawaz, Guanlin Xie - Effects of thidiazuron
on In vitro plant regeneration and radical scavenging activity in Brassica rapa var. turnip
PPBB 17 Kaleem Ullah Kakar, Zerqa Nawaz, Bilal Haider Abbasi - Plant regeneration from seed
derived callus of medicinally important Brassicarapa var. rapa
PPBB 18 Laila Fayyaz, Farhatullah, Sikandar Shah, Sidra Iqbal, and Mehwish Kanwal - Analysis of
genetic variability in interspecific progenies of Brassica napus/ Brassica campestris using (SSRs)
PPBB 19 Madiha sadiq and Rehana Asghar - Study of polypeptides induced by drought stress in some
local varieties of barley (Hordeum vulgare L.)
PPBB 20 Manazza Shahzad, Rahmatullah Qureshi, Mehmooda Munazir, Muhammad Arshad and
Muhammad Gulfraz - Antibacterial activity of leaves extract of Moringa oleifera lam. from thal
desert, Pakistan
PPBB 21 Mansoor Ahmad, Zahoor Ahmad Swati, Imtiaz Ali Khan and Muhammad Naeem - Morphobiochemical characterization of brassica oilseed genotypes
PPBB 22 Mansoor Ahmad, Zahoor Ahmad Swati, Muhammad Naeem and Imtiaz Ali Khan - Morphobiochemical losses in brassica oilseed genotypes due to aphids
PPBB 23 Mubashir Niaz, Tehreema Iftikhar and Muhammad Hassan Raza - Eco-cultural optimization
for glucoamylase production by Penicillium chrysogenum under solid substrate conditions
PPBB 24 Mubashir Niaz, Tehreema Iftikhar and Sidra - Comparative studies on the biosynthesis of
triglycerol acyl hydrolases by wild & mutant derivatives of Fusarium sp.
PPBB 25 M.N. Khalid and Mustafa Shameel - Studies on the phycochemistry and biological activity of
Spirogyra rhizoides (chlorophycota)
PPBB 26 M. Farooq Hussain Munis, Asghari Bano, Hassan Javed Chaudhary, Muhammadi Optimization of quantitative real-time PCR analysis for reliable detection and quantification of
Fusarium oxysporum in wheat
PPBB 27 M. Shahid, C. Dumat, B. Pourrut, C. Laplanche, J. Silvestre and E. Pinelli - Early steps of
lead-induced oxidative stress to vicia faba roots: role of lead speciation
PPBB 28 Muhammad Ali and Firdaus-e-Bareen - Indigenous microorganisms from the macroenvironment consistence system of bamboo
PPBB 29 Muhammad Ashraf, Abdul Ghafooor and Guo Dong Wei - Androgenesis through isolated
microspore culture for the production of haploid plants in wheat (Triticum aestivum L.)
PPBB 30 Muhammad Ishtiaq, Tanveer Hussain and Mehwish Maqbool - Bioinformatics and its
application in agriculture and plant systematics
PPBB 31 Muhammad Abdul Rab Faisal Sultan, Shahjahan Shabbir Ahmed, Imran Ahmad and
Mudassar Khan - Mineral profile analysis of indigenous feedstuff for dairy animals
PPBB 32 Muhammad Din, Abdul Ghani and Muhammad Younas Khan Barozai - Identification of
micrornas from 12 plant species of Fabaceae
PPBB 33 Nadar Khan, Muhammad Tehseen Zaheer Tanoli, Naushad Ali, Malik Ashiq Rabbani,
Abdul Ghafoor and Muhammad Shahid Masood - Diversity in seed storage proteins in oat
(Avena sativa L.) germplasms from Pakistan-I: Variation in 12S globulin acidic subunits
PPBB 34 Naila Ali and Humera Afrasiab - In vitro germination and callogenesis of safflower (Carthamus
tinctorius L .)
PPBB 35 Najamuddin Solangi, Adel Ahmed Abul-Soad & Ghulam Sarwar Markhand - Somatic
embryogenesis protocol for mono-embryonic mango (Mangifera indicaL L.) varieties
PPBB 36 Neelma Munir, Faiza Khan, Sumera Javed, Farah Aslam and Shagufta Naz - Optimization
of microwave assisted extraction from Bryophyllum leaves and antifungal response of crude
extracts

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PPBB 37 Neelma Munir, Faiza Khan, Sumera Javed, Farah Aslam and Shagufta Naz Standardization of culture conditions for various algal species and effect of various solvents on
the lipid yield
PPBB 38 Pakeeza Amber, Abida Akram and Zahid Akram - Molecular evaluation of Sclerotium rolfsii
isolates from chickpea through rapd primers
PPBB 39 Raja Tahir Mahmood, M. Javaid Asad, Nazia Mehboob, Saqib H.H., M. Asghar and M.
Gulfraz Solid state fermentation of corn stover by A. Sydowii for the production of indigenous
exoglucanase
PPBB 40 Riaz-ur-Rehman, Muhammad Faheem, Muhammad Saleem Akhtar, Abid Mahmood Morphogenic influence of explants to TDZ and other phytoharmones on the organogenesis of
Dahlia variabilis Red Skin
PPBB 41 Rifat Hayat, Iftikhar Ahmed, Yeseul Sin, Jayoung Paek, Muhammad Ehsan, Akira Yokota,
Muhammad Iqbal and Young H. Chang - A moderately boron-tolerant candidatus novel soil
bacterium lysinibacillus pakistanensis sp. nov. cad., isolated from soybean rhizosphere (Glycine max L.)
PPBB 42 Roheena Abdullah, Ikram-ul-Haq, Zahid Ali Butt, Tehreema Iftikhar and Mehwish Iqtedar
- Strain improvement and optimization of media for the production of alpha amylase by
Aspergillus oryzae
PPBB 43 Rukhsana Jabeen - Potential of azadirachtin-d fraction against Xanthomonasoryzae pv.
Oryzaecause bacterial leaf blight disease in rice
PPBB 44 Sabahat Anwar and Humera Afrasiab - Effect of different growth regulators and media on
callus induction in amla (Emblica officinalis)
PPBB 45 Saba Asad, Madeehahussain, Ayesha Siddequa, Qurrat-ul-Ain and Habib Bokhari Detection of molecular markers by comparative sequence analysis of enzymes from
Mycobacteria species
PPBB 46 Sadaf Naseem, and Ghazala Nasim - Biocontrol of Euphorbia Helioscopia using fungal
pathogens
PPBB 47 Sadia Intzaar, Muhammad Akram and Humera Afrasiab - Micropropagation and shoot
proliferation of pygmy groundcherry (Physalis minima L.): A threatened medicinal herb
PPBB 48 Sadia Ishaq, Imran Hashmi, Zarrin Fatima Rizvi and Shaukat Farooq - The effect of high
concentrations of methyl parathion at various temperatures and ph values on growth of
pseudomonas IES-PS-1
PPBB 49 Sadia Rizwan and Faheem Aftab - Effect of different pretreatments on breaking seed dormancy
and In vitro germination in Jatropha curcas L.
PPBB 50 Safida Anwar, and Ghazala Nasim - Intercomparison of rhizospheric microbial flora of wild
and modified varieties of some economically important cereal crops
PPBB 51 Sahib Gul Afridi, Habib Ahmad Imtiaz A. Khan and Mukhtar Alam - Phytochemical
characterization of tea genotypes based on their main quality componenets
PPBB 52 Saira Jabeen and Ghazala Nasim - Increase in rice (Oryza sativaL L.) seed vigor and
subsequent growth through potassium humate application
PPBB 53 Sana Khan and Ghazala Nasim - Disease constraints of hydroponically grown ginger (Zingiber
officinale Rosc.)
PPBB 54 Sarwat Iftikhar, Amjad ur Rahman, Yasir Ihtesham, Uzma Khan and Rizawana Aleem
Qureshi - Antioxidant and antimicrobial potential of Ajuga bracteosa L., and Otostegia limbata
L., against pathogenic microorganisms
PPBB 55 Shaista Khan, Allah Bux Ghanghro, Farah N. Talpur, AN. Memon, M.S. Memon and
Ibtessam Tahir - Quantitative analysis of wheat proteins in different varieties grown in Sindh,
Pakistan
PPBB 56 Shahida Perveen, Rahmatullah Qureshi, Noshin Ilyas, Gul Rahim and M. Gulfraz Preliminary phytochemical screening and antioxidant potential of Cymbopogon jwarancusa
(Jones) Schult.
PPBB 57 Shakra Jamil, Ihsan Ullah, Muhammad Zaffar Iqbal and Sobia Jabeen - A simple genotypeindependent protocol for direct regeneration and transformation in Indian mustard
PPBB 58 Shumaila Tahir, Asghari Bano, Warda Shahid, M. Aqeel Kamran, Amna, Rabya Mufti and
Hassan Javed Chaudhary - Ethno medicinal and Pharmacological Investigation of Araucaria
columnaris Hook and Cycas revoluta Thunb.
PPBB 59 Siddra Sardar, Safdar Hussain Shah, Azhar Hussain Shah and Zahoor A. Swati - Effects of
osmotic and ionic stresses on regeneration capacity of rice (Oryza sativa L.) calli
PPBB 60 Tahsin Gulzar, Sadaf Ambreen, Muhammad Younas and Zafar Iqbal - Assesment of genetic
relationships among wheat genotypes by rust resistant markers and bioinformatics tools

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PPBB 61 Tehreema Iftikhar, Mubashir Niaz, Qamar Batool and Muhammad Anjum Zia Biosynthesis of triacylglycerol acyl hydrolases by Penicillium citrinum through submerged
fermentation technique
PPBB 62 Tehreema Iftikhar, Mubashir Niaz and Maria Naqvi and Rukhsana Jabeen - Biosynthesis of
amyloglucosidase by Penicillium citrinum using solid substrate fermentation technique
PPBB 63 Tehreema Iftikhar, Mubashir Niaz, Asma Tariq and Mariam Iftikhar - Comparative studies
on the biosynthesis of triacylglycerol acylhydrolases by a wild and mutant strain of Penicillium
chrysogenum through submerged fermentation technique
PPBB 64 Tehreema Iftikhar, Mubashir Niaz, Yasir Hussain and Zaib un Nisa - Comparative studies on
the biosynthesis of triacylglycerol acylhydrolases by a wild and mutant strains of Aspergillus
niger through submerged fermentation technique
PPBB 65 Tehreema Iftikhar, Mubashir Niaz, Hina Sarwat and Faiza Akram - Comparative studies on
the lipase biosynthesis of wild and mutant strains of Trichophyton sp. (MBL 23) through
submerged fermentation
PPBB 66 Tehreema Iftikhar, Mubashir Niaz, Shamoona Shahid and Muhammad Asad ullah Fazal A comparative account of two ascomycetous fungi for the biosynthesis of b-D-fructofuranoside
fructohydrolase through solid state fermentation technique
PPBB 67 Tehreema Iftikhar, Mubashir Niaz, Qurat-ul-Ain Rahat and Muhammad Anjum Zia Process optimization for the biosynthesis of b-D-fructofuranoside fructohydrolase by a locally
isolated culture of Alternaria alternate
PPBB 68 Tehreema Iftikhar, Mubashir Niaz, Muhammad Khan Rafiq and Rukhsana Jabeen Studies on the biosynthesis of triacylglycerol acyl hydrolases by Penicillium sp. isolated from
pickle (MBL-40) through solid substrate fermentation technique
PPBB 69 Tehreema Iftikhar, Mubashir Niaz, Anila Nureen and Roheena Abdullah - Production,
partial purification and characterization of triacylglycerol acyl hydrolases by Fusarium sp. under
submerged fermentation techniques
PPBB 70 Tehreema Iftikhar, Mubashir Niaz, Anam Anwar and Zahid Butt - Process optimization for
the biosynthesis of b-D-fructofuranoside fructohydrolase by a locally isolated culture of Rhizopus
oligosporus
PPBB 71 Tehreema Iftikhar, Mubashir Niaz, Maria Aleem and Amber Imtiaz - A comparative
account of fermentation techniques for the biosynthesis of triacyl glycerol acly hydrolases by a
wild strain of Bacillus subtillus
PPBB 72 Tehreema Iftikhar, Mubashir Niaz and Rabiya Ashraf and Maryam Yousaf - A comparative
account of two ascomycetous fungi for the biosynthesis of Triacyl glycerol acyl hydrolases
through solid substrate fermentation technique
PPBB 73 S. Ahmed and U. Ilyas - Optimization of extraction process of cmcase from fermented matrix of
Vigna mungo in solid state fermentation
PPBB 74 Umbreen Rashid, Muhammad Rashid Khan, Shumaila Jan, Jasia Bokhari, Naseer Ali Shah
and Bushra Ahmad - Phytochemical analysis and study of antibacterial, antifungal and brine
shrimp cytotoxicity activities of Fagonia olivieri (dc).
PPBB 75 Umera Dogar, Dr. Khan Rass Masood and Arooj Naseer - Bisaccate pollen from late permian,
chhidru formation, western salt range, Pakistan
PPBB 76 Waseem Safdar, Hamid Majeed and Barkat Ali - In-vitro clonal propagation of
Withaniasomnfera: An important medicinal plant
PPBB 77 Waseem Safdar, Hamid Majeed and Barkat Ali - In vitro micropropagation of an important
medicinal plant: Wattakaka volubilis L.
PPBB 78 W. Ijaz, M. Ahmed, Fayyaz-ul-hassan, Muhammad Aqeel Aslam and Muhammad Aslam Dynamic modeling of phosphorous under changing climate
PPBB 79 Zahid Ali Butt, Ikram-ul-Haq, Hamid Mukhtar, Roheena Abdullah and Maqsood Ahmad Production of alginate by azotobacter vinelandii EMS-45 in a stirred fermentor
PPBB 80 Zohra Bibi, Mukhtar Ahmed, F.U. Hassan and Jabar Zaman Khan Khattak - Statistical and
dynamical modeling of wheat (triticum aestivum L.) fungal diseases under climate change
PPBB 81 Zanib Ahmed, Muhammad Asgher and Hafiz Muhammad Nasir Iqbal - Comparative study
on bioethanol production from Saccrharomyces cerevisiea through alkali and enzymatic
hydrolysis of sugarcane bagasse
PPBB 82 Ikram-ul-Haq, Uzma Hameed and Sardar Junaid Bahadur Khan - Exploration of Candida
species for the production of glucoamylase using agricultural by-products
PPBB 83 Ikram-ul-Haq, Uzma Hameed and Maha Khan - Development of fungal consortium for the
production of endo-1,4--glucanase

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PPBB 84 Uzma Hameed, Fareeha Raza, Ikram-ul-Haq and Mehmood Ali Khan - Lactose as
alternative inducer for the production of recombinant -amylase from Thermotoga petrophila
under the influence of T7 promoter
PPBB 85 Muhammad Mohsin Javed, Muhammad Asjad Khan, Muzaffar Javed, Umer Karim, Sana
Zahoor and Ikram-ul-Haq - Potential of microbial and plants -glucans toward lowering of
LDL and enhancing of HDL
PPBB 86 Sana Zahoor, Muhammad Mohsin Javed and Ikram-ul-Haq - Biotechnologically important
thermophilic bacterium isolated from the hot spring of Azad Kashmir, Pakistan
PPBB 87 Zahoor Ahmad Sajid and Faheem Aftab - Plant regeneration from in vitro-selected salt tolerant
callus cultures of Solanum tuberosum L.
PPBB 88 Arooj Yousaf Khan and Zakia Latif - Screening of medicinal natural extratcs for their
antibacterial activity against Salmonella species

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Theme 3: Plant Physiology / Stress Physiology / Microbial Interactions

Oral Presentations
OPPP 1
OPPP 2
OPPP 3
OPPP 4
OPPP 5
OPPP 6
OPPP 7
OPPP 8
OPPP 9
OPPP 10
OPPP 11
OPPP 12
OPPP 13
OPPP 14
OPPP 15

OPPP 16
OPPP 17

Abid Riaz, Farooq A. Shah, and Tariq M. Butt - Intra specific variability among Metarhizium
anisopliae strains in their ability to produce balstospores in liquid culture media
Abdul Razzaq, QasimAli, Abdul Qayyum, Imran Mahmood, Muhammad Ahmad,
Muhammad Rasheed - Physiological Responses and Drought Resistance Index of Nine Wheat
(Triticum aestivum L.) Cultivars under Different Moisture Conditions.
Adnan Riaz, Abid Riaz, Farah Naz and Javed Asad - Phenylalanine ammonia-lyase and
peroxidase activity in brown rust infected tissues of pakistani wheat cultivars
Amjad Iqbal, Zahid Ullah and Mushtaq Ahmad - Presence of bioactive compound in lepidium
sativum L., exudates: Role in allelopathy
Amanullah and B.A. Stewart - Shoot to root ratio differ in warm season C4-cereals in response
to plants competition under low and high water levels
Ammara Ahad, Asma Maqbool and Kauser A. Malik - Agrobacterium tumefaciens mediated
optimization of transformation in Eucalyptus camaldulensis
Aasma Tufail, Muhammad Arfan, Ali Raza Gurmani and Asghari Bano - Salicylic acid
induced salinity tolerance in maize (Zea Mays)
Armghan Shahzad, Sami Ashraf, Farwa Karamat, Muhammad Iqbal, Jalal-ud-Din A.
Mujeeb-Kazi and Ghulam Muhammad Ali - Quatitative trait loci (QTLs) mapping of drought
tolerance at germinaiton stage in a wheat population derived from synthetic hexaploid and opata
Chandni Yaqoob, Humera Aslam Awan, Asma Maqbool and Kauser Abdulla Malik Microbial diversity of the rhizosphere of kochia (Kochia indica) growing under saline conditions
Erum Mukhtar, Ejaz Hussain Siddiqi, Khizar Hayat Bhatti, Khalid Nawaz and Khalid
Hussain - Gas exchange attributes can be valuable selection criteria for salinity tolerance in
canola cultivars (Brassica napus L.)
Fakhra Shamim, Giles N. Johnson, Abdul Waheed, Habib-ur-Rehamn Athar and S. M.
Saqlan Naqvi - Higher antioxidant capacity protects photosynthetic activities as revealed by chl a
fluorescence in drought tolerant tomato genotypes
Farhana Kausar and Muhammad Shahbaz - Interactive effect of foliar application of nitric
oxide (NO) and salinity on wheat (Triticum aestivum L.)
F.A. Bughio, S.M. Mangrio, T.M. Jahangir, S.A. Abro and Hadi Bux - Physio-morphological
responses of native Acacia nilotica to Eucalyptus allelopathy
G.S. Channa, A.R. Mahar, I. Rajpar, Zia-ul-hassan, J.H. Umrani and W.A. Maitlo Comparing salinity tolerance of five high yielding, non-aromatic rice (Oryza sativa L.) cultivars
of Sindh
Ghazala Nawaz, Muhammad Jamil, Muhammad Mudasar Aslam, Shakirullah Khan,
Sajjad Asaf, Lubna, Amber Gul and Shafiq-ur-Rehman - Effect of plant derived smoke
solution on adventitious roots of Ipomoea marguerite, Eppipremnum pinnatum and Rosa indica in
comparison with auxin (indol-3-butyric acid)
Hira Kalim, M.Farman, Asghari Bano, Tanveer Ahmad and Nadeem Ahmed - Study of
phytochemical constituents from Ricinus communis Linn., roots and their effect on different
strains of bacteria and fungi
Humera Aslam Awan, Ahmad Zaheer, Asma Imran, Sajjad Mirza and Kauser Abdullah
Malik - Spatial metagenomic analysis of bacterial community associated with wheat rhizosphere
grown in rhizobox

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OPPP 18
OPPP 19
OPPP 20
OPPP 21
OPPP 22
OPPP 23
OPPP 24
OPPP 25
OPPP 26
OPPP 27
OPPP 28
OPPP 29
OPPP 30

OPPP 31
OPPP 32

OPPP 33
OPPP 34
OPPP 35
OPPP 36
OPPP 37

Humaira Yasmin and Asghari Bano - Screening of PGPR isolates from semi-arid soil and their
implication to alleviate drought stress
Ihsanullah Daur and Ahmed A. Bakhashwain - The effects of humic acid on the growth and
quality of maize fodder under organic management
Imtiaz Ahmad Qamar, Maqsood Ahmad, Gulshan Riaz and Sartaj Khan - Performance of
summer forage legumes and their residual effect on subsequent oat crop in subtropical subhumid
Pothwar (Pakistan)
Jaffar Ali, Najma Yaqub, Faheem Aftab - In vitro development and improvement of chromium
(vi) affected adventitious roots of solanum tuberosum l. With GA3 and IAA application
Jehan Bakht, Shehla Khan and Mohammad Shafi - Antimicrobial potentials of fresh Allium
cepa against gram positive and gram negative bacteria and fungi
Muhammad Adnan, Asif Ahmad, Anwaar Ahmed, Nauman Khalid, Imran Hayat, Iftikhar
Ahmed - Chemical composition and sensory evaluation of tea (Camellia Sinensis)
commercialized in Pakista
M. Ahmed, Fayyaz-ul-Hassanand Muhammad Aslam - Proline accumulation in bread wheat
(Triticum aestivum L.) under different environmental conditions
Muhammad Ashfaq Anjum, Zahir Ahmad Zahir, Sher Muhammad Shehzad, Muhammad
Arshad and Muhammad Ashraf - Precursor (L-tryptophan)-inoculum (rhizobia) interactions for
improving growth, yield and nodulation of mung bean (Vigna radiata L.)
Muhammad Kamran, Abdul Latif Khan, Qari Muhammad Imran, Amana Khatoon,
Muhammad Waqas, Noreen Imran, In-Jung Lee and Shafiq-ur-Rehman - Effect of plant
extracted smoke and reversion of abscisic acid stress on lettuce
Muhammad Saleem, M. Y. Ashraf, Sabir Ali Sabir, M. Rizwan, Khalid Mahmood and J.
Akhtar - Evaluating plant water-use efficiency and yield of coarse rice advance lines/varieties for
soil with limited moisture content
Muhammad Yahya Khan, Hafiz Naeem Asghar, Muhammad Usman Jamshaid and Zahir
Ahmad Zahir - Effect of microbial inoculation on plant growth and remediation of chromium
contaminated soil
Qudsia Bano, Noshin Ilyas, Asghari Bano, Nadia Zafar, Abida Akram, Fayaz ul Hassan and
Muhammad Arshad - Effect of Azospirillum inoculation on maize (Zea mays L.) under drought stress
Rifat Hayat, Iftikhar Ahmed, Yeseul Sin, Jayoung Paek, Muhammad Ehsan, Akira Yokota,
Muhammad Iqbal and Young H. Chang - A moderately boron-tolerant candidatus novel soil
bacterium Lysinibacillus Pakistanensis sp.nov.cad., isolated from soybean rhizosphere (Glycine
max L.)
Saba Anwar, Muhammad Iqbal, Hafiz M. Akram, Naeem Iqbal and Mubashir Niaz Influence of drought applied at different growth stages on the yield and qualitative traits in maize
Sami Ullah Khan, Ayub Khan, Ali Raza Gurmani, Jalal-Ud-Din ,Fayyaz-Ul-Hassan,
Muhammad Saeed, Hakim Khan, Muhammad Liaqat, Sher Aslam and Abdul Qayyum - Oil
yield, fatty acid profile, seed yield and yield attributes of sunflower as influenced by autumn
planting conditions in Islamabad
Seema Mahmood, Shabnam Ishtiaq, Muhammad Ibrahim Malik and Ali Ahmed Differential growth and photosynthetic responses but similar pattern of metal accumulation in
sunflower (Helianthus annuus L.) cultivars at elevated level of lead and mercury
Shahzad Munir, Zabid Ullah, Mehwesh Afreen, Noureen Banoori, Iqbal Nisa, Qaiser Jamal,
Rashid Azim Khan and Muhammad Anees - Chitinolytic activity of indigenous Trichoderma
spp. against different fungal phytopathogens
Shamim Akhtar, Armghan Shahzad, Muhammad Arshad, Fayyaz-ul-Hassan and
Rahmatullah Qureshi - Morpho-physiological evaluation of groundnut (Arachis hypogaea L.)
Genotypes for iron deficiency tolerance
Ahmad, S., M. Khalid, S.S. Akhtar and M.B. Hussain - Inoculation of Rhizobium
leguminosarum with ACC-deaminase containing PGPR for improving growth, nodulation and
yield of lentil
Wazir Ali Maitlo, Ghulam Sarwar Markhand, Adel Ahmed Abul-Soad and Abdul Mubeen
Lodhi - Chemical control of sudden decline disease of date palm (Phoenix dactylifera L.) in
Sindh, Pakistan

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Aftab Wajid, Ashfaq Ahmad, Gerrit Hoogenboom, Tasneem Khaliq and M. Usman Modeling growth, development and seedcotton yield of promosing cotton cultivars at varying

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nitrogen increments with different planting dates under DSSAT

Aftab Wajid, Ashfaq Ahmad, Javed Iqbal, Tasneem Khaliq and Gerrit Hoogenboom Evaluation of oilcrop-sun model for different planting densities at various nitrogen rates under
semi-arid conditions of Punjab-Pakistan
A. Majeed, Riaz A. Mann, M. Saleem, A. Wahab, M. Asim and A. Bano - Water-saving rice
production using alternate wetting and drying technique in rice based cropping system in Sindh,
Pakistan
Alamgir Alvi, M. Amjad Qureshi, N. Akhtar, A. Iqbal, F. Mujeeb and L. Ali - Potential of
rhizobium inoculation to enhance the yield and nutrient use efficiency of spinach
Ali Raza Gurmani, Asghari Bano, Najeeb Ullah, Ayub Khan, Jinlin Zhang and T.J. Flowers
- Exogenously applied silicate and abscisic acid ameliorates the growth of salinity stressed wheat
(Triticum aestivum L.) seedlings through Na+ exclusion
Ameer Khan, Zara Shaheen and Amin Shah - Amelioration of salt stress in wheat (Triticum
aestivum L.) by foliar application of nitrogen and potassium
Amna Imran and A.N. Khalid - Ectomycorrhizal diversity of himalayan spruce (Picea
Smithiana)
Amna Shoaib, Nida Aslam, Saba Khurshid, Sundus Akhtar and Nafisa - Green mould as
potential scavenger of Cr(VI)
A. Kanwal, A. Wahid, M. Farhan and Y. Zhao - ICPS-87- risk assessment of lead toxicity
through growth performance of wheat plants in Punjab, Pakistan
Annum Khalid, Naeem Iqbal, M. Yasin Ashraf, Makhdoom Hussain, Javeed Anwar and
Muhammad Khalid Tanvir - Acclimation of cimmyt wheat (Triticum aestivum L.) Lines to
rainfed enviroment: Water use efficiency and grain yield
Arooj Naseer, Khan Rass Masood and Umera Dogar - Seed ferns flora from early permian
(artinskian), amb formation, western salt range, Pakistan
Asghari Bano, Asia Nosheen and Faizan Ullah - Plant growth promotion and seed quality
enhancement of Ethopian mustard as influenced by bioinoculants supplemented with mineral
fertilizers
Asim Shehzad and Asghari Bano - Effects of microbes in Biofilm and their effects on plant
growth
Ather Mahmood and Muhammad Iqbal - Effect of pre-anthesis ascorbic acid application on
the post-anthesis high temperature stress tolerance in spring wheat
Atif Riaz, Adnan Younis, Shoaib Munir , Mansoor Hameed, Asif Riaz Taj and Sitwat Riaz Effect of drought stress on growth and flowering of marigold (Tagetes erecta)
Awais Afzal, Muhammad Irfan and Kauser Abdulla Malik - Molecular diagnostics of
foodborne pathogens (Salmonella sp.)
Azra yasmeen, Shahzad Maqsood Ahmed Basra, Muhammad Mansoor Javaid, Hafeez-urRehman, Abdul Wahid and Nazim Hussain - Effect of exogenous application of natural and
synthetic growth enhancers on quantitative and qualitative attributes of tomato (Lycopersicum
esculentum)
Edwin John, Asma Maqbool and Kauser A. Malik - Agrobacterium Tumefaciens mediated
optimization of transformation in Populus deltoids
Ejaz Ashraf, Yasir Iftikhar, Samiullah and Zahoor Hussain - Impact of different cultivation
practices by citrus growers on citrus greening disease in Sargodha, Pakistan
Fakhar Mujeeb, M. Amjad Qureshi, N. Akhtar, A. Iqbal, A. Alvi and L. Ali - Interactive
effect of phosphate solublizing bacteria and rhizobium inoculation on berseem
Farkhanda Jabeen and Maria Bashir - Isolation and characterization of chlorpyrifos degrading
bacteria from the agricultural soils
Fazal Hadi, Muhammad Arif and Farrukh Hussain - Effect of different nitrogen levels and
cutting on growth behavior of dual purpose barley
Ghulam Abbas, Zahid Hussain, Muhammad Saqib, Muhammad Atiq-ur-Rehman,
Muhammad Arshad, Khalil Ahmed and Muhammad Atif Riaz - Effect of salinity on growth
and ionic composition of Acacia nilotica
Ghulam Nabi, M. S. Akhtar, M. Mahmood-ul-Hussan and M. Memon - Plant growth
attributes of rice cultivars under parachute transplanting
Hidayatullah, M. Memon, Saeed Ahmed and Tariq Ziad - Molybdenum application promotes
nodulations and yield of peas
Iqbal Hussain, Sobia Qurat-ul.Ain, Rizwan Rasheed, Saqib Mahmood and Muhammad

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Iqbal - Response of maize seedling to time and cadmium application

Iram Shahzadi, Naeem Iqbal, Saleem Akhtar, Sajid-ur-Rahman and Zafar Iqbal Somaclones development for higher wheat (triticum aestivum L.) grain yield: Growth and
biochemical attributes
Jalal-ud-Din, Sami ullah, Noor Elahi Jan, Ahmad Khan and Ali Raza Gurmani Physiological and biochemical responses of tomato (Lycopersicon esculentum Mill.) to high
temperature stress
Khalil Ahmed, Muhammad Saqib, Javaid Akhtar, Rashid Ahmad - Interactive effects of
salinity and boron toxicity on growth and physiological parameters of maize
Khizar Hayat Bhatti, Aqsa Azam, Norin Jahan, Amin Shah, Kahlid Nawaz, Khalid
Hussain, Ejaz Hussain Siddiqi and Qaisar Mehmood - Cadmium toxicity attenuation by
parthenium: A case study
Maqsood Ahmad, Abdul Wahid, Zahid Ali Butt and Yuechun Zhao - Ascertaining the impact
of hexavalent chromium through wheat (Triticum aestitvum L.) Plants in Punjab, Pakistan
Maria Maqsood, Humera Aslam Awan, Asma Maqbool and Kauser Abdulla Malik Microbial diversity and its salt tolerance in rhizosphere of Suaeda fruticosa
Mehwesh Afreen, Rashid Azim, Naureen Banoori, Iqbal Nisa, Qaiser Jamal, Zabid Ullah
and Muhammad Anees - Biocontrol potential of the indigenous Trichoderma isolates against
plant pathogenic strains of Fusarium oxysporum
Motsim Billah, Matiullah Khan, Tanveer Ahmed and Asia Munir - Effect of P-enriched
compost on growth and yield of wheat (Triticum aestivum L.) and its residual impact on
groundnut (Arachis hypogeal L.) productivity
Muhammad Abdul Rab, Faisal Sultan , Liu Hui , Zhao Hui Xian - The screening of water
stress tolerant wheat cultivars with physiological indices
Muhammad Adeel, Humera Aslam Awan, Asma Maqbool and Kauser Abdulla Malik Study of microbial diversity in cotton (Gossypium hirsutum) rhizosphere
Muhammad Akhtar, Asif Naeem, Faqir Hussain, M.Yasin Ashraf, Javed Akhter and K.
Mahmood - Enhancing phosphorus use efficiency in cereals by phosphoric acid application in
alkaline calcareous soils
M. Akhtar and B. Ghazala - Taxonomic study of freshwater chlorophycota and euglenophycota
from tehsil kasur
M. Amjad Qureshi, A. Iqbal, F. Mujeeb, S. Jamil, N. Akhtar, A. Alvi and L. Ali - Microbial
populations and organic carbon flux after the application of weedicides in wheat
Muhammad Ahmed Akram, Zahir Ahmad Zahir and Muhammad Baqir Hussain - Impact
of multi-strain inoculation on growth and productivity of rice
Muhammad Aqeel and Mumtaz Hussain - Response of mungbean [Vigna radiata (L.)
Wilczek.] to cadmium and nickel applied as soil treatment
Muhammad Baqir Hussain, Zahir Ahmad Zahir and Sajid Mahmood - Impact of plant
growth promoting rhizobia on growth, physiology and yield of maize
Muhammad Akram And Faheem Aftab - Fruit size and sampling sites reveal useful
information on seed dormancy, viability and germination in teak (Tectona grandis L.)
M. Amjad Qureshi, A. Iqbal, L. Ali, Armghan Shehzad and A. Khan - Oilseeds and PGPR:
Potential combination for phytoremediation
Muhammad Amjad Qureshi, A. Iqbal, N. Akhtar, A. Khan and L. Ali - Precursor (Ltryptophan)-inoculum (Rhizobium) interaction for promoting wheat yield
Muhammad Atiq-Ur-Rahman, Muhammad Saqib, Khawer Saleem, Ghulam Abbas,
Muhammad Arshad, Khalil Ahmed and Muhammad Atif Riaz - Effect of salinity on growth
and macro and micro nutrient uptake of different wheat (Triticum aestivum L.) genotypes
M. A. Shakir, Jamil S., Sohail I., Jamil M and A. Bano - Saline agriculture an innovative
strategy for enhancing land and water availablity in South Punjab
M. Shahzad, M. Anwar-ul-Haq and J. Akhtar - Behavior of different wheat (Triticum aestivum
L.) genotypes in saline soil
M. Tariq Javed, Sabaz A. Khan, A. Bano, T. Landberg and M. Greger - Relevance of
cadmium and proton stress in Elodea canadensis for phytofiltration
M. Yasin Ashraf, M. Ashraf, M. Akhter and Javed Akhter - Improvement in fruit yield,
quality and fruit droping control in kinnow (Citrus reticulata Blanco) through application of
growth regulators, potassium and zinc
N. Bangash, A. Khalid, T. Mahmood and T. Siddique Screening rhizobacteria containing
ACC-deaminase for growth promotion of wheat seedlings under water stress

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N. Batool, M. Arshad and Fayyaz-ul-Hassan - Effects of siliqua position on phsico-chemical

composition of Brassica napus L., seeds
Nabeela Abid, Aftab Bashir, Asia Khatoon, Muhammad Irfan, Nufaid Khan, Mehwish
Asharf, Zeeshan Majeed, Asma Maqbool and Kauser Abdulla Malik - Development of
transgenic wheat with low phytate for increasing bioavailability of iron and zinc
Nadeem Ahmed - Toxicity of lead, chromium and cadmiummetal ions in vegetables irrigated
with city effluent of Islamabad
Naseem Akhtar , M. Amjad Qureshi, S. Jamil, A. Iqbal, F. Mujeeb and L. Ali - Phosphate
solubilizing potential of Rhizobium and Bacillus species for enhancing available phosphorus in
maize crop
Nazila Azhar, M.Y Ashraf, M. Ashraf, A. Hameed and M. Hussain - Response of antioxidant
enzymes activities in sunflower (Helianthus annuus L.) to EDTA grown in Pb contaminated medium
Nizamuddin Depar, Inayatullah Rajpar, Muhammad Yousuf Memon, Javaid Ahmed Shah
and Muhammad Afzal Arain - Biomass accumulation and potassium substitution by sodium of
rice genotypes under salinity stress in hydroponics
Noor Elahi Jan, Jalal-ud-Din and Saneyuki Kawabata - Impact of saline-alkali stress on the
accumulation of soluble solids in tomato fruits
Rafia Mubaraka, Zahir Ahmad Zahir, Muhammad Baqir Hussain and Muhammad Khalid
- Screening competent rhizobial isolates for combating salinity stress in wheat seedlings
Raheela Rehman, Kausar Nawaz Shah, M. Shahid Masood, Muhammad Arshad, M. Fahim
Abbas and Abdul Ghafoor - Genetic divergence among pakistani bread wheat varieties and
advanced lines on randomly amplified polymorphic DNA (RAPD) markers
Ratooba S. Hashmi, Nafees A. Khan, Samiullah, Habib Ahmad, Khajawa S. Ahmad and
Muhammad M. Qayyum - Influence of nitrogen fertilization on leaf area, photosynthesis and oil
yield of japenese mint (Mentha arvensis L.)
Roshan Zamir, Shahid Akbar Khalil, Syed Anwar Shah, Syed Tariq Shah, Nisar Ahmad
and M. Tariq - Performance of sugarcane genotypes/germplasm against frosttolerance, high cane
and sugar yield under the agro climatic conditions of Peshawar valley
Sabeeh-ur-Rasool Sabir, Mumtaz Hussain and Muhammad Aqeel - Morpho-physiological
responses of wheat (Triticum aestivum L.) to simulated acid rain and micro nutrients
Sabaz Ali Khan, Muhammad Tariq Javed, Muhammad Shahzad, Asghari Bano, Henk J.
Schouten and Evert Jacobsen - Acidity is an important determinant for fruit taste in apple
Sadaf Niaz, Tanveer Akhtar, Muhammad Shafiq, Nuzhat Sial, Abdul Ghaffar and Abul
Hasanat - Punica granatum (Anar, fruitrind) as an alternative medicine for parasitic diseases
Samiullah, Asghari Bano, Sisay Girmay and Ghee Tan - Sulforhodamine b (SRB) cell
proliferative assays of Suaeda fruticosa against human lung carcinoma (LU-1) and hormone
dependent prostrate carcinoma (LNCAP)
S.A. Khuhro, K.S. Memon and M. Memon - Assessing potassium nutrition status of sugarcane
through soil and plant analysis
Amna Imran and A.N. Khalid - Ectomycorrhizal diversity of Himalayan spruce (Picea
smithiana)
S. Memon, K.S. Memon and M. Memon - Effect of pressmud on the growth, yield and
chemical composition of maize
Sehrish Jamil, M. Amjad Qureshi, N. Akhtar, A. Iqbal, F. Mujeeb and L. Ali - Effect of
Rhizobium and PGPR inoculation on the growth and yield of Soybean (Glycine max L.)
Shahid Akbar Khalil, Roshan Zamir, Nisar Ahmadand Syed Anwar Shah - High frequency
indirect plants regeneration from leaf explants in Stevia rebaudiana (Bert.)
Shahida N. Khokhar, Mohammad Ali Khan, Aftab Afzal and Rizwan Ahmad - Grain yield
and Nitrogen distribution in wheat in response to inoculation by Azospirillum spp., and
Azorhizobium spp in two different soils of Pakistan
Shah F.R., N. Ahmad, K.R. Masood and J.R. Peralta-Videa - Physiological effect of the
wastewater of Hudiara drain on Dalbergia sissoo (Roxb.)
Shamyla Nawazish, Mumtaz Hussain, M. Ashraf and M.Y. Ashraf - Phyto-monitoring of
metal pollution released by automobiles along Motorway (M-3) and National Highway (N-5) in
Pakistan
Shazia Iram, Sehrish Iqbal and Kousar Parveen - Heavy metal remediation by fungi
Sobia Zulfiqar and Muhammad Shahbaz - Response of canola (Brassica napus L.) to foliar
applied triacontanol under saline and non-saline conditions
Tamoor-ul-Hassan and Asghari Bano - Physiological and biochemical charaterization of

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Tanveer Ahmad, M.Farman, Asghari Bano, Hira Kalim and Nadeem Ahmed Phytochemical screening of four different root extracts of Ageratum conyzoides Linn. and
potential role in antibacterial and antifungal activity
Urva-Til-Vusqa, Noshin Ilyas, Hafsa Aman, Muhammad Arshad and Azeem Khalid Evaluation of plant growth promoting rhizobacteria associated with wheat under drought stress
Umair Akhtar, Humera Aslam Awan, Asma Maqbool and Kauser Abdulla Malik - Effect of
pgrs (n2 fixers) on growth of wheat
Yasir Iftikhar, S.M. Mughal, M.M.Khan, M.A. Khan, M.A. Nawaz and Z. Hussain Symptomatic expression of tristezainfected citrus trees in Pakistan
Zabta Khan Shinwari, Nisar Ahmad, Javid Hussain, Razia Perveen and Haider Ali Antimicrobial evaluation and proximate profile of Nepeta leavigata, Nepeta kurramensis and
Rhynchosia reniformis
Zahid Ali and Fauzia Yusuf Hafeez - Plant growth promoting rhizobacteria (PGPRs) enhance
seedling vigor and seedling growth in cotton
Zahoor Ahmed, Asghri Bano, Ali Raza Gurmani, Sami Ullah Khan, Nowsherwan Noble
Nawab and Jalal-ud-Din - Gibbrellic acid alleviates adverse effects of salinity stress by
optimizes ions and increases growth and yield of peas (Pisum Sativum L.)
Zia-ur-Rehman Mashwani, Mir Ajab Khan, Mushtaq Ahmad, Muhammad Zafar Classification of forage grasses species based on mineral composition by principal component
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Theme 4: Plants & Environment

Oral Presentations
OPPE 1
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OPPE 3
OPPE 4
OPPE 5
OPPE 6
OPPE 7
OPPE 8
OPPE 9
OPPE 10
OPPE 11
OPPE 12
OPPE 13
OPPE 14
OPPE 15

Alia, Bushra Jabeen, Shahida N. Khokhar, Aftab Afzal and Saeed A. Asad - Phosphate
solubilizing bacteria associated with vegetables roots in different ecologies
Amir Muhammad Khan, Mian Muhammad Anwar-ul-Hassan Tahir, Altaf Ahmed Dasti,
Shafiq u Rehman, Rizwana Aleem Qureshi, Ishtiaq Hussain, Syed Aneel Gilani, Faizan
Ullah and Rehan Naeemand Waheed Murad - Life form diversity in vegetation of the
cholistan desert
Amna Bano, Sher Akbar, Gul Bano, Mudassar Israr Zaidi, Rabeea Zafar - Study of
pollutants (toxic/heavy metals) in some edible fruits of Quetta
Amna shoaib, Nida Aslam, Sundus Akhtar and Saba Khurshid - Green mold as a potential
scavenger of CR(vi)
Ashfaq Nazir, Riffat Naseem Malik and Hamayun Shaheen - Pytosociological studies of the
vegetation of sarsawa hills district Kolti, Azad Kashmir
Beenish Malik and Sheikh Saeed Ahmad - An appraisal of ecological distribution of
herbaceous flora at gatwala forest park (GFP), Faisalabad, Pakistan
Kanwal Waqar, Iftikhar Ahmad, Rehana Kausar, Tuseef Tabassum and Muhammad Ashiq
- Use of bioremediated sewage effluent for fish survival
Farooq Ahmad, Amin U. Khan and Abdullah Yasar - The Potential of Chlorella vulgaris for
wastewater treatment and biodiesel production
Iram Shahzadi, Azeem Khalid, Shahid Mahmood, Tariq Mahmood and Muhammad
Arshad - Effect of ACC deaminase containing bacteria on growth of wheat seedlings applied
with chromium contaminated water
Muhammad Ali Khan, Noor-ul-Amin, Sikandar Hayat, Muhammad Sajid, Imran Ahmad,
Asif Shah and Muhammad Rezaul Kabir - Therapeutic horticulture: influencing psychological
responses of surgical patients and their environmental assessment scale
Muhammad Farhan, Abdul Wahid, Amina Kanwal and J.N.B. Bell - Synthesis of activated
carbon from tree sawdust and its usage for diminution of color and cod of paper-mill effluents
Muhammad Ibrar Shinwari, Maryum Ibrar Shinwari and Yoshiharu Fujii - Allelopethic
evaluation of shared invasive plants and weeds of pakistan and japan for environmental risk
assessment
Munir Ozturk, Serdal Sakcali, Salih Gucel, Ali Celik and Volkan Altay - Diplotaxis tenuifolia
l. (dc) as a biomonitor of heavy metal pollution alongside the roads in Turkey
Nazish Javaid and Muhammad Ajaib - Antioxidant and antimicrobial activities of an
ethnobotanically important plant Helinus lanceolatus Wall. ex Brandis of district Kotli, Azad
Jammu & Kashmir
Noshin Arif, Mehwish Jamil Noor and Rabia Tariq - Effect of particulate matter on leaf

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pigment contents, stomata and leaf area of selected roadside plant species of Sialkot
OPPE 16 Rafiq Ahmad, Yasmine Zuily-Fodil, Chantal Passaquet, Olivier Bethenod and Anne
Repellin - Bacterial expression, purification and partial characterization of new recombinant
cysteine protease from maize leaves: Post-transcriptional changes under ozone stress
OPPE 17 Saadullah Khan Leghari, Mudassir Asrar Zaidi and Abdul Kabir Khan Achackzai - Effect
of air pollution on the leaf morphology of common plant species of quetta city
OPPE 18 Sardar Khan, Alia Naz, Shaikh Saeed Ahmad and Saeeda Yousaf - Toxicity and
bioaccumulation of heavy metals in spinach seedlings grown on freshly contaminated soil
OPPE 19 Sehrish Sadia, Shahida Khalid, Rahmatullah Qureshi, Sana Riaz, Yasir Arafat and Tauseef
Anwar - Allelopathic interactionof Tagetes minuta L. - an environmentally safe bioherbicide
OPPE 20 Shazia Iftikhar, Sadia Kanwal and Iftikhar Ahmad - Wastewater and soil quality assessment
of Nullah Lai of Pakistan
OPPE 21 Shazia Iftikhar, Sadia Kanwal and Iftikhar Ahmed - Accumulation of heavy metals by In
vitro cultures of plants
OPPE 22 Shazia Iram, Amarra Arooj and Kousar Parveen - Tolerance potential of fungi isolated from
polluted soil of Multan
OPPE 23 Shazia Iram, Khadija Basharat and Iftikhar Ahmad - Tolerable analysis of the fungi of the
peri-urban agricultural area
OPPE 24 Shazia Iram, Sehrish Iqbal and Kousar Parveen - Heavy Metal Remediation By Fungi
OPPE 25 Shereen Khaliq, Azeem Khalid, Beenish Saba, Shahid Mahmood and Muhammad T.
Siddique - Effect of ACC deaminase bacteria on tomato plants containing azo dye wastewater
OPPE 26 Syeda shaima Meryem, Azra Yasmin -Effects of lead resistant bacteria on the early growth of
vigna mungo L. (Hepper) under lead stress
OPPE 27 Sumera Farooq - Mangrove ecosystem threats and management
OPPE 28 Tauseef Anwar, Shahida Khalid, Yasir Arafat, Sehrish Sadia and Sana Riaz - Management
of Avena fatua l. And Rumex dentatus L., in associated crops with plant extracts
OPPE 29 Volkan Altay, Ibrahim Ilker Ozyigit, Mustafa Keskin, Goksel Demir and Ibrahim Ertugrul
Yalcin - An ecological study of endemic plant Polygonum istanbulicum keskin and its environs
OPPE 30 Waqas Hussain, Zahid Akram, Talat Mehmood, Ghulam Shabbir, Rehmatullah Qureshi, U.
Shaukat and Abdul Mujeeb Kazi - Evaluation of molecular mapping population from
wheat/synthetic hexaploid cross for drought tolerance
OPPE 31 Ilhan Dogan, Ibrahim Ilker Ozyigit, Azim Ozturk, Serdal Sakcali, Guzin Kekec, Goksel
Demir, Filiz Vardar, Ebru Artam Tarhan and Sezen Igdelioglu - Physiological and genotoxic
alterations induced by boron in sunflower Helianthus annuus L.
OPPE 32 Ibrahim Ilker Ozyigit, Ilhan Dogan, Bulent Eskin, Mustafa Keskin, Goksel Demir and
Ibrahim Ertugrul Yalcin1 - Mineral nutient uptake status of endemic isoetes anatolica prada &
rolleri populations from Bolu, Turkey
OPPE 33 Saeed Ahmad Asad, Scott Young and Helen West- Effect of Ni and Cd on glucosinolate
production in Thlaspi caerulescens
OPPE 34 Rafiq Ahmad, Yasmine Zuily-Fodil, Ulrike Jana and Anne Repellin- The influence of
earthworm apporrectodea caliginosa on the iron transport in two species of arabidopsis
OPPE 35 Jam Nazeer Ahmad, Sandrine Eveillard and Rafiq Ahmad- Interaction of defense pathways in
two different isolates of stolbur phytoplasma-infected tomato
OPPE 36 Jam Nazeer Ahmad, Rafiq Ahmad, Lilly Maneta Peyret and Patrick Morau- Study of lipidprotein interaction in the secretory pathway of plant cell by raising and using anti-lipid antibodies
against particular lipids and proteins in arabidopsis and tobacco plants
OPPE 37 Sardar Khan, Alia Naz, Said Muhammad and Salma - Heavy metals toxicity and their
bioaccumulation in purslane (Portulaca oleracea) seedlings grown in the green house
enviornment
OPPE 38 Bulent Eskin, Ibrahim Ilker Ozyigit, Ilhan Dogan, Volkan Altay, Goksel Demir and Memduh
Serin - Some physiological and autecological features of Centaurea kilaea Boiss., from Turkey
OPPE 39 Goksel Demir, Ibrahim Ilker Ozyigit, Ilhan Dogan, Bulent Eskin and Mustafa Keskin Mineral elements in Veronica scutellata L., (Grassleaf speedwell) from Bolu-Turkey: Soil-plant
interactions
OPPE 40 Ibrahim Ilker Ozyigit, Azim Ozturk, Ebru Artam Tarhan, Sezen Igdelioglu and Goksel
Demir - The effects of Ni on Helianthus annus L., tissue cultures in different developmental
phases
OPPE 41 Ilhan Dogan, Ibrahim Ilker Ozyigit, Azim Ozturk, Serdal Sakcali, Guzin Kekec, Goksel

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Demir, Filiz Vardar, Ebru Artam Tarhan and Sezen Igdelioglu - Physiological and genotoxic
alterations induced by boron in Helianthus annuus L. (Sunflower)

Poster Presentations
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Aasma, Muhammad Zakria, Shahzad Asad and Atif Jamal - Prevalence of karnal bunt of
wheat in Punjab and Khyber Pakhtunkhwa (Pakistan)
Adnan Younis, Atif Riaz, Muhammad Qasim, Muhammad Nadeem and Mansoor Hameed Naturalistic planting in urban landscape increase biodiversity
Asma Hassan, Shahzada Sohail Ijaz, Safdar Ali, Muhammad Ansar, Khalid Saifullah Khan
and Qaiser Hussain - Tillage and crop sequence effect on soil organic carbon fractions and
aggregate stability in dryland Pothwar, Pakistan
Adeela Nazar and Mehwish Jamil Noor - Temporal variation in chlorophyll A, B and
carotenoid in selected tree species of district Chakwal
Afroz R. Khan, Mohammad Saeed Safia Gull, Tahira Mengle and Ruksahana Jabeen Phytochrome mediated germination responses in red.far red irradiated Cucurbita pepo L., and
Citrullus vulgaris T seeds under different temperatures
Afroz R. Khan, Tahira Mengle, Safia Gull, Ruksahana Jabeen and Mohammad Saeed Allelopathy effect of Melia azedarach L., on test specie
Ahmet R. Mermut - Carbon stocks, cycling, sequestration and food security
Ali Abulgasim El Agael - Efficacy of libyan isolates of Bacillus subtilis against Fusarium
oxysporum and Rhizoctonia solani
Ali Noman, Qasim Ali, Mansoor Hameed, Tahir Mehmood and Naeem Iqbal - Inter-cultivar
genetic potential of Hibiscus rosa-sinensis for adaptation to changing environmental conditions in
relation to various leaf anatomical characteristics
Ammara rauf, Farooq A. Khan, Muhammad Aslam and Muhammad Nadeem Anwar Assesment of salt tolerance among different sunflower accessions
Amna Bano, Sher Akbar, Gul Bano, Rabeea Zafar and Juma Khan Kakar - Study of
pollutants (Toxic/Heavy metals) in some edible fruits of Quetta
Aqsa Azam, Khizar Hayat Bhatti, Noreen Jahan, Amin Shah, Qaisar mehmood, Ejaz
Hussain Siddiqi, Khalid Nawaz, Khalid Hussain and Ishtiaq Hussain - Nickel toxicity
mitigation from soil by parthenium using as phytoremediation tool
Asifa Shafeeq, Zahid Ali Butt, Sohaib Muhammad and Maqsood Ahmed - Impact of
different nickel concentrations on growth, biomass and some yield characterstics of wheat
(Triticum aestivum L. var. Shafaq-06)
Asif Sajjad, Shafqat Saeed, Muhammad Amjad Bashir and Asad Masood - Floral host plant
range of butterflies (Lepidoptera) at Multan, Pakistan
Asrar Ali, Ehsan-ul-Haq, Abdul Rehman, Waseem Gillani, Javed Khan and Maria Rauf Biological parameters and predatory potential of Menochilus sexmaculatus Fab. (Coleoptera:
Coccinellidae) at varying temperature on Rhopalosiphum padi L.
Aysesha Tania, Abida Akram, Nafeesa Qudsia Hanif, Muhammad Arshad and Abdul Rauf Nutritional profile, mycoflora assessmesnt and afaltoxin contamination in chickpea (Cicer arietinum L.)
Fahim Arshad, Khan Rass Masood And Tahira Malik - Late palaeocene pteridophytic spores
from patala formation, western salt range, Pakistan
Fahim Nawaz, Rashid Ahmad, Muhammad Yasin Ashraf, Ejaz Ahmad Waraich and
Muhammad Ashraf - Effect of rate and duration of selenium seed primingon growth of wheat
(Triticum aestivum L.) seedlings under drought stress
Faisal Hafeez, Waseem Akram, Unsar Naeem-Ullah, Khuram Zia and Hafiz Azhar Ali
Khan - Biocidal properties of citrus oils against dengue mosquito Aedes albopictus
Faiza Shaikh, Afsheen Zehra, M. Zaheer Ahmed, Bilquees Gul, Raziuddin Ansari and M.
Ajmal Khan - Impact of dormancy regulating chemicals on seed dormancy of three medicinally
important grasses
Farah-Anwar Dal, Abdul Hayee Memon, Muhammad Shaiq Ali, S.M. Leghari and Mehnaz
Dal -Taxonomic and phycochemical investigation of Gloeocapsa compacta from the ponds of
district Badin, Sindh
Farzeen Khan, Irfan Aziz and M. Ajmal Khan - Comparative effects of exogenous glycine
betaine and ascorbic acid on gas exchange and leaf sclerophylly indices of Ceriops tagal under
salt stress
Ghazala Nasim and H.M. Waqas - Mycographic analysis of macromycetes of Ayubia National
Park, development of identification software and indication of threatened species

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Ghazala Shaheen, Mudassir Asrar Zaidi, Sadulla Laghari and Fahim - Role of epidermal
morphology in the identification of some medicinal plants of Quetta
Hamd Meer, Shazia Iram, Iftikhar Ahmad, Faisal Sohail Fateh and Munawar Raza Kazmi
- Identification and characterization of post harvest fungal pathogens of mango from domestic
markets of Punjab
Iffat Tahira, Salma Khatoon, Nafeesa Qudsia Hanif, Nighat Sultana and Kishwar Nazir
Sultana - Mycotoxigenic fusarium spp., and fusariotoxin in maize grains, collected from different
climatic zone of Pakistan
Iftikhar Ahmed, Muhammad Kamran Haider, Muhammad Iqbal, Hina Javed, Qanita
Rashid, Shazia Erum, Nauman Khalid, Muhammad Zakria and Ghulam M. Ali - Molecular
identification, phylogenetic analysis and biochemical characterization of endophytic bacterial
population associated with citrus canker infected leaves in Pakistan
Ijaz Ahmed Khan, Address Khan and Rahimdad - Effect of different plants extracts and
herbicides on infesting flora and yield and yield components of chickpea
Iqnaa Naseer and Muhammad Sajid Aqeel Ahmad - Growth analysis of some chickpea (Cicer
arietinum L.) Lines under salt stress
Irfan aziz, Farzeen Khan and M. Ajmal Khan Contribution of compatible osmolytes and
antioxidant enzymes in salt tolerance of Avicennia marina
Kaniz fatima, Khan Rass Masood and Riffat Jabeen - Palynological characterization of tobra
formation from burihkel section, western salt range, Pakistan
Kaniz Fatima, Khan Rass Masood and Fahim Arshad - Palynology of early triassic shale from
khan zaman nala, western salt range, Pakistan
Khadija Rafiq and Firdaus-e-Bareen - Role of EDTA in enhancement of heavy metal uptake
and their root to shoot partitioning in Helianthus annuus
Khadim Hussain, Shafqat Saeed and Malik Saeed Ahmed - Effect of earthworm processed
farmyard manure on the growth and yield of tomatoes
Kiran Munir, Rani Faryal, Fauzia Y. Hafeez and Abdul Hameed - Oven dried biomass of
indigenous A. niger: A candidate biosorbent for bimetal removal
Maria Rauf, Ehsan-ul-Haq, Abdul Rehman, Waseem Gillani, Javed Khan and Asrar Ali Biology and predatory potential of coccinella septempunctata linn. On green bug Schizaphis
graminum R., under controlled conditions
Mehwish Jamil Noor and Mir Ajab Khan - Mellissopalynological studies of honeybee flora of
Islamabad, Pakistan
Mubashir Niaz, Tehreema Iftikhar, Muhammad Kamran Bashir and Lal Khan Babar Integrated fertilizer and pesticide management for Bt-and Non-Bt-cotton (Gossypium hirsutum L.)
Muhammad Adnan, Asif Ahmad, Anwaar Ahmed, Nauman Khalid, Imran Hayat and
Iftikhar Ahmed - Chemical composition and sensory evaluation of tea (Camellia sinensis)
commercialized in Pakistan
Abdul Hameed, Salman Gulzar, Bilquees Gul and M. Ajmal khan - Growth, water relations
and photosynthetic responses of a coastal halophyte Limonium tocksii to salt stress
Muhammad Aqeel and Mumtaz Hussain - Response of mungbean [Vigna radiata (L.)
Wilczek.] To cadmium and nickel applied as soil treatment
Muhammad Fiaz, Habib Ahmad,Abdul Nasir Khalid - Addition of Telial stages of Cerotelium
and Phakopsora species (Basidiomycota; Uredinales from Pakistan
M. Shahid, C. Dumat, J. Silvestre and E. Pinelli - Lead-induced toxicity to Vicia faba pigment
contents: Comparison of free and complexed lead
M. Shahid, E. Pinelli, C. Laplanche, B. Pourrut, J. Silvestre and C. Dumat - Role of metal speciation
in early steps of lead-induced ROS production and lipid peroxidation in Vicia faba L., seedlings
M. Hussain Khajak, Mohammad Saeed, Dost M. Baloch, M. Naeem Shahwani, Nazeer
Ahmed and Agha. M. Raza - A comparative study of cone and seed morphological
characteristics of Juniperus excelsa in balochistan, Pakistan
Muhammad Ibrar Shinwari, Maryum Ibrar Shinwari and Yoshiharu Fujii - Phytotoxicity
evaluation of medicinal plants and weeds for agro-environment risk assessment: Leaf-litter by
sandwich method
Muhammad Shoiab Amjad, Zahid Hussain Malik and Nafeesa Zahid Malik - Phenological
patterns among the vegetation of ganga chotti and bedori hills in a moist temperate to alpine forests
Muhammad Shoaib Amjad, Malik Zahid Hussain and Nafeesa Zahid Malik - High altitude
forest composition diversity and its component in a part of Ganga Chotti and Bedori hills district

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bagh. Azad Jammu and Kashmir, Pakistan

Muhammad Shoaib Amjad, Nafeesa Zahid Malik, Zahid Hussain Malik and Muhammad
Arshad - Life form and leaf size spectra reported in moist temperate forest of Pir-Chinassi hills,
District Bagh Azad Jammu & Kashmir
Muhammad Shoaib Amjad, Zahid Hussin Malik, Nafeesa Zahid and Muhammad Arshad Aggregation and regeneration capacity of vegetation in Kotli hills Azad Jammu and Kashmir
Muhammad Shoaib Amjad, Zahid Hussain Malik, Sidra Rafique and Nafeesa Zahid Malik
- Phytosociology of some weeds of wheat communities around kotli fields western himalaya
Muhammad Tasdiq Hussain Shahid, Farooq Ahmad Khan, F. M. Azhar, Bilquees Fatima
and Muhammad Aslam - Variability assessed in red rot resistant somaclones of sugarcane
genotype S97 US297 in R1 and R2 generations
Munazzah Meraj, Sadia Javed, Rao Irfan and Khalil-ur-Rahman - Controlling ammonia
volatilization from urea surface applied to loamy soil
Nafisa Shoaib and Pirzada J.A. Siddiqui - Sensitivity of some marine cyanobacterial species to
methyl parathion (Organophosphate pesticide)
Noor-ul-Ain Soomro, Basir Ahmed Arain, M.T. Rajput and Nabila Shah Jilani Ougenioxylon chinjiensis sp. Nov., a new fossil species of the family Leguminosae from chinji
formation salt range, Punjab Pakistan
Qamar Saeed, Syed Muhammad Zaka, Shoaib Freed and Shafqat Saeed - Lucerne as trap
crop in wheat for development of predators population against wheat aphids (Aphidaidae:
Homoptera)
Riffat Jabeen, Khan Rass Masood and Samia Akram - Early eocene filicinean spores from
the Ghazij formation, north east Balochistan, Pakistan
R. Khanum and A. S. Mumtaz - The ecogeographic analysis of wild Asclepiads of Pakistan
S. Ahmed, A. Wahid and S. S. Ahmad - Responses of various canola cultivars against ambient
ozone pollution
Saba Iftikahr, Tariq Mahmood, Irfan Aziz, Beenish Saba and Azeem Khalid - Status of
Biological carbon under different land uses in Rawalpindi district
Sabeeh-ur-Rasool Sabir, Mumtaz Hussain and Muhammad Aqeel - Morpho-physiological
responses of wheat (Triticum aestivum L.) to simulated acid rain and micro nutrients
Sadaf Naseem and Ghazala Nasim - Biocontrol of Euphorbia helioscopia using fungal
pathogens
Sadia Sagheer, Khan Rass Masood, Fahim Arshad and Riffat Jabeen - Miospore assemblage
(Trilete) from tredian formation (Zaluch gorge), Western Salt Range, Pakistan
Saeed Akhter Abro, Amanullah Mahar, Sher Mohammad Mangrio and Farooque A.
Bughio - Exotic conocorpus plantations and urban ecology of hyderabad city: A threat analysis
Safida anwar and Ghazala nasim - Inter-comparison of rhizospheric microbial flora of wild and
modified varieties of some economically importanat cereal crops
Sanam Asif, Abida Akram, Nafeesa Qudsia Hanif, Rahmatullah Qureshi and Abid Riaz Assessment of seed-borne mycoflora and nutritional profile of lentil (Lens culinaris) grown in
arid region
Sana Khan and Ghazala Nasim - Disease constraints of hydroponically grown ginger (Zingiber
officinale Rosc.)
Sana Riaz, Shahida Khalid, Riaz Chattha and Sehrish Sadia - Role of Carica papaya L., in
reducing environmental hazards of herbicides through allelopathy
Sana Riaz, Shahida Khalid, Riaz Chattha, Sehrish Sadia, Yasir Arafat and Tauseef Anwar Weed management by Cuscuta L. (parasitic plant)
Saira Jabeen and Ghazala Nasim - Increase in rice (Oryza sativa L.) seed vigor and subsequent
growth through potassium humate application
Saira Bano, Muhammad Aslam, Shahzad M.A. Basra and Khalid Aziz - Physiological
response of different maize accessions at early growth stage under low temperature stress
Sahar Khalid, Zaheer-ud-din Khan and Sohaib Muhammad - Assessment of carbon storage
and sequestration in kathar game ecological reserve
Sardar Khan, Alia Naz and Shaikh Saeed Ahmad - Toxicity and bioaccumulation of heavy
metals in spinach seedlings grown in freshly contaminated soil
Sehrish Sadia, Shahida Khalid and Rahmatullah Qureshi - The intriguing behavior of Tagetes
minuta L.
Shahid George, Nyla Shuja, Shazia Iram, Iftikhar Ahmad and Ehsan Akhtar - Effect of ultra
violet light on chromium tolerant isolate of Aspergillus niger

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Shahid George, Tariq Mahmood, Irfan Aziz and Beenish Saba - Effect of temperature
fluctuations on fate of organic matter in soils of semi-arid region
Shazia Iram, Amarra Arooj and Kausar Perveen - Metal tolerance potential of fungi isolated
from polluted soil of Multan
Shazia Iram, Mehreen Naz, Ambreen Aziz and Kausar Perveen - Tolerance and genetic
variations among different species of fungi from contaminated soils
Shujaul M Khan, Sue Page , Habib Ahmad, Zahidullah, Mushtaq Ahmad and David
Harper -Phyto-climatic gradient of vegetation and habitat specificity of indicator species in the
high elevation Western Himalayas
Sidra Akram, Azeem Khalid, Tariq Mahmood and Muhammad Arshad - Effects of ACCdeaminase bacteriaon growth and yield of wheat (Triticum aestivum) under rainfed conditions
Sumera Rasheed and Mehwish Jamil Noor - Temporal variation in leaf pigment contents in
selected tree species growing along road Major networks in Rawalpindi
Sundas Imtiaz Qureshi and Azra Yasmin - Bahaviour of lentil seedlings in the presence of lead
and lead resistant bacteria
Syed Attique Hussain, Muhammad Ishtiaq, Zaheer Ahmed and Mehwish Maqbool - Effect of
auto-exhaust emission on the morphological and anatomical characters of Cassia siamea and Solanium
nigrum L., growing in different areas of district Gujrat Pakistan and district Bhimber Azad Kashmir
Tahira Malik , Khan Rass Masood and Fahim Arshad - Lower triassic palaeoclimatic
interpretations based on palynological data from Mianwali formation, Western Salt range, Pakistan
Tauseef Anwar, Shahida Khaldand Yasir Arafat - Euphorbia helioscopia L., as a source of
bio-herbicide
T.Z. Shamsi and A. Jabeen - Ethnobotanical survey of selected towns of Gujranwala, Punjab
U. Younis, S.A. Malik, S. Niaz, R. Raja and S. Mahmood - Dust accumulation capacity and
alteration of various biometric and biochemical traits in cultivated populations of Ficus carica L.
Zafar Farooqui, Seema Shafique and Pirzada J. A. Siddiqui - Growth of mangrove plants,
Avicennia marina and Rhizophora mucronata, growing in the Indus Delta (Hajambro Creek)
Zahid Ullah, Mushtaq Ahmad, Muhammad Ilyas, Mir Ajab Khan and Muhammad Zafar Phytodiversity, life form, communities and species distribution pattern in mountain ecosystem of
mian dam Pakistan

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Theme 5: Taxonomy & Ethnobotany

Oral Presentation
OPTE-1

Abdul Nazir, Mir Ajab Khan, Farooq Ahmad, Mushtaq Ahmad ,Muhammad Zafar,
Mansoor Hameed, Tahira Nawaz, Khawaja Shafique Ahmad and Shabnum Shaheen Foliar epidermal features as an aid to the identification of grasses of Tribe Andropogoneae
(Poaceae) from Pothohar region of Pakistan
OPTE-2 Akbar Ali Meo and Mir Ajab Khan - Palynological diversity of echinops in the tribe cardueae
(Astereae) from Pakistan
OPTE-3 Ali elik - Endemic plants of aydin mountains and conservation strategies
OPTE-4 Ali elik, Kutret Gezer, Ouzhan Kaygusuz and Ahmet Ermi - Effect of Gypsophila
pilulifera Boiss. & Heldr., over breeding of pleurotus ostreatus (Jacq. Ex Fr.) Kumm.
OPTE-5 Amin Shah, Shahzad Hussain, Khizar Hayat Bhatti, Noor-ud-Din, Mushtaq Ahmad,
Muhammad Zafar, Ameer Khan and Muhammad Ishtiaq - An ethnobotanical study of
Medicinal plants in Semi-Tribal Area, Kurd Sharif & Sho (District Karak (Khyber
Pakhtunkhawah)
OPTE-6 Arshad Mehmood Abbasi, Mir Ajab Khan, Mushtaq Ahmad, Muhammad Zafar, Ghulam
Mujtaba Shah, Kirn Yasmin Khan and Rabia Niamat - Ethno-medicinal assessment of some
selected wild edible fruits and vegetables of Lesser-Himalayas, Pakistan
OPTE-7 Asad Ullah, Abdur Rashid and Syeda Nighat Parveen - Linking plant biodiversity with
conservation and livelihood improvement of inhabitants of Kalash Valley, District Chitral
OPTE-8 Farooq Ahmad, Mir Ajab Khan, Mushtaq Ahmad, Mansoor Hameed, Muhammad Ashraf,
Muhammad Zafar, Muhammad Arshad, Tahira Nawaz and Khawaja Shafique Ahmad Tetrapogon cenchriformis and Parapholis strigosa, (Poaceae), two new reports from Pakistan
OPTE-9 Ghulam Mujtaba Shah, Mushtaq Ahmed, Manzoor Hussain, Zafar Jamal, Arshad
Mahmood Abbasi and Muhammad Zafar - Phytotherapy among the rural women of
Abbottabad District Northern Pakistan
OPTE-10 Habib Ahmad - Floristic diversity and vegetation distribution in the siren river catchment Pakistan

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OPTE-11 Hamayun Shaheen, Zahid ullah and Mushtq Ahmad - Indigenous phyto-remedies practiced to
cure feminine diseases in tribal communities of Kashmir Himalayas
OPTE-12 Humaira Saeed, Ghulam Mujtaba Shah, Manzoor Hussain, Habib Ahmed and Shazia
Sultana - Ethnobotanical studies and conservation status of trees of district Abbottabad, Pakistan
OPTE-13 Khalid Ahmad, Mushtaq Ahmad, Zia-ur-Rehman Mashwani, Muhammad Yousaf Ethnobotonical apprisial in southern plains of Takht-e-Suleman Hills
OPTE-14 Malik Farooq Ahmed, Mir Ajab Khan, Mushtaq Ahmed, Muhammad Zafar and Hafiz
Muhammad Baksh - Floristic inventory of Khabaki wetland ramsar site of the salt range wetland
complex, Valley Soon Sakesar District Khushab
OPTE-15 Muhammad Ayub, Mushtaq Ahmad, Muhammad Zafar, Sofia Rashid, Haleema Sadia and
Abida Bano - Ethnobotany and conservation status of floral biodiversity of Himalayan Range of
Azad Jammu and Kashmir, Pakistan
OPTE-16 Muhammad Ibrar Shinwari, Misbah-u-Din, Zafeer Saqib, Maryum Ibrar Shinwari Sustainability assessment of traded medicinal plants of Beha Valley in Swat - Pakistan
OPTE-17 Muhammad Ishtiaq Ch., Mehwish Maqbool and Tanveer Hussain - Role of indigenous
knowledge in biodiversity conservation of an area: A case study on tree ethnobotany of soona
valley, district Bhimber Azad Kashmir, Pakistan
OPTE-18 Munir Ozturk, Aykut Guvensen1, Salih Gucel and Volkan Altay - An overwiev of the
atmospheric pollen in Turkey and the Northern Cyprus
OPTE-19 Niaz Mohammad Tareen, Tahira Mengal, Saeed-ur-Rehman, Uzma Khan, Rasool Bukhsh
Tareen, Mushtaq Ahmad and Afroz Rais - Womens indigenous knowledge about medicinal
plants of kuchlak area district Quetta, Balochistan, Pakistan
OPTE-20 Qamar Abbas, Rehmat ullah Qureshi, Sher Wali Khan, Arifun Nissa Naqvi , Mushtaq
Ahmad and Ishtiaq Hussain - Floral inventory and ethnobotanical study of Naltar Valley Gilgit
district, Pakistan
OPTE-21 Rahmatullah Qureshi - Flora of Khanpur Dam, Punjab, Pakistan
OPTE-22 Sarfaraz Khan Marwat, Mir Ajab Khan, Khalid Usman, Mushtaq Ahmad and Muhammad
Zafar - Floristic Account of emergent-aquatic and marshland angiosperms of D.I. Khan district,
KPK, Pakistan
OPTE-23 Shabnum shaheen, Nidaa Haroon, Sehrish Ramzan, Farah Khan and Zaryab Khalid Systematic Identification of genus Brachiaria on the basis of vegetative and floral morphoPalynological markers (LM & SEM)
OPTE-24 Shamila Firdous, Habib Ahmed, Manzoor Hussain and Muqarrab Shah - Pollen morphology
of Ajuga L., Lamium L. and Phlomis L. from Abbottabad district
OPTE-25 Shazia Sultana, Mir Ajab Khan, Mushtaq Ahmad, Asghari Bano, Muhammad Zafar and
Zabta Khan Shinwari - Taxonomic and phytochemical authentication of herbal drug Barg-eHenna (Lawsonia innermis): A multiuse medicinal plant
OPTE-26 Shujaul M Khan, Sue Page, Habib Ahmad, Zahidullah, Mushtaq Ahmad and David Harper
- Phyto-climatic gradient of vegetation and habitat specificity of indicator species in the high
elevation Western Himalayas
OPTE-27 Siraj Ahmed Kakar, Rasool Bakhsh Tareen, Zia-ud-Din Sandhu, M Azam Kakar, Zafar
Iqbal, Saeed ur Rehman Kakar, Hameeda Jabeen and Shumaila Irum - In vitro and In vivo
anthelmintic activity of Ferula costata (Kor.) against gastrointestinal nematodes of sheep
OPTE-28 Syed Qaiser Abbas, Mubashir Niaz, Iftikhar Ali, Nabila Iftikhar, Rubab Ayesha and Tehreema
Iftikhar - Addition to fungi on Azadirachta Indica from Faisalabad and Gojra, Pakistan
OPTE-29 Tahira Mengal, Rasool Bukhsh Tareen, Zahoor Ahmad Bazai, Saeed-ur-Rehman Kakar,
Mir Ajab Khan, Mushtaq Ahmad, Muhammad Zafar, Niaz Mohammad Tareen and
Rukhsana Jabeen - An ethnobotanical study of district Mastung, Balochistan, Pakistan
OPTE-30 Yunus Dogan, Ilker Ugulu and Nazmi Durkan - Wild edible plants sold in the local markets of Izmir
OPTE-31 Zafar Jamal, Mir Ajab Khan, Manzoor Hussain, Ghulam Mujtaba Shah, Mushtaq Ahmad
and Muhammad Zafar - Ethnomedicinal plants used for gastrointestinal ailments by the rural
communities of Kaghan Valley, Mansehra, Pakistan
OPTE-32 Zahid ullah, Mushtaq Ahmad, Mir Ajab Khan, Muhammad Zafar and Richard Milne Phylogeny of carex L. (cyperaceae) from Pakistan based on matk gene sequence variation
OPTE-33 Zia-ur-Rehman Mashwani, Sadia Irum, Mir Ajab Khan, Mushtaq Ahmad and Muhammad
Arshad - Antioxidant potential of root bark of berberis lycium royle from Western Himalaya, Pakistan

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Poster Presentations
PPTE- 1

Akbar Ali Meo and Mir Ajab Khan - Pollen morphology as an aid to the identification of the
taxa of Tribe Vernonieae (Asteraceae)

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PPTE- 19
PPTE- 20
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PPTE- 22
PPTE- 23
PPTE- 24
PPTE- 25
PPTE- 26
PPTE- 27
PPTE- 28
PPTE- 29

Akbar Zeb, Shujaul Mulk Khan, Habib Ahmad, Gul Jan, Farzana Gul Jan
Mushtaq Ahmad and Hayat Ullah - Ethnomedicinal studies of dughalgay valley district swat,
Khyber Pakhtoonkhwa, province, Pakistan
Aman khan, Gul Jan, Muhammad Hamayun, Farzana Gul Jan, Mushtaq Ahmad,
Muhammad Zafar, Mohib Sha and Masood Jan - Diversity and conservation status of
medicinal plants of Naran valley Khyber Pakhtoonkhwa province, Pakistan
Atta Msohammad Sarangzai, Muhammad Youns khan barozai, Saeed ur Rehman and Alia
Ahmed - Ethnobotany of Juniperus excelsa in Balochistan Province, Pakistan
Aykut Guvensen, Salih Gucel, Volkan Altay and Munir Ozturk - Atmospheric pollens in
Turkey and their allergic effects
B. Ghazala S. Ahmad R. Raja - Taxonomical studies of Bacillarales of fish farms from Multan
city, Pakistan
Basir Ahmed, Nabeela Shah, Noorulain Soomro and Tahir - Dipterocarpoxylon ranilotensis Sp.
Nov. A new taxon of Dipterocarpiaceae from Ranikot fort area, district Jamshoro, Sindh Pakistan
Brian Gagosh Nayyar, Abida Akram, Muhammad Arshad, S.M. Mughal, Shaista Kkhund Mycoflora detected from seeds of Sesamum indicum L. in Sialkot
F. Zerrin Saltan and zer zaydn - Ethnobotany of Eskiehir and its environs
Faizul Haq, Zafar Iqbal, Habib Ahmad and Rahat Ullah - The ethnobotanical uses of trees
and shrubs in District Battagram, Khyber Pakhtunkhwa, Pakistan
Farkhanda Jabeen and Hira Ishfaq - Studies on antimicrobial activity of some selected
medicinal plants
Farzana Gul Jan, Muhammad Hamayun, Gul Jan, Mir Ajab Khan, Mushtaq Ahmad,
Muhammad Zafar and Mohib Sha - Ethnoveterinary plant remedies used by Gujjar
Communities in Northern areas of Pakistan
Farzana Muhammad Ali, Zaheer-ud-din Khan and M. Ajaib - An ethnofloristic study of the
plants of District Nankana Sahib, Pakistan
Gul Jan, Mir Ajab Khan, Farzana Gul Jan, Muhammad Hamayun, Mushtaq Ahmad,
Muhammad Zafar, Rafiullah Khan and Aman Khan - Exploitation and prospects for
conservation: Plant diversity of Dir Kohistan Valley (Khyber Pakhtoonkhwa), Pakistan.
Hafsa Raheem, Tahira Mengal, Saeed-ur-Rehman, Rasool Bukhsh Tareen, Mushtaq
Ahmad Niaz, Mohammad Tareen and Safia Gul - Ethno-medicinal plants used to cure women
diseases by women of Khanozai district Pishin, Balochistan, Pakistan
Iftikhar Ahmad Khan and Habib Ahmad - Conservation issues of tree flora in natural habitats
of totalai tract ditrict Buner
Javed Imran, Zhang ShuoXin, Shahjahan Shabbir Ahmed, Muhammad Abdul Rab Faisal
Sultan, Khadim Hussain and Yuan Jie, Cheng Fei - Sustainable forest management (SFM) in
Pakistan: history, problems and challenges
K. Sultana, Naveeda Riaz, Gulshan and Ali Nauman Khan - Contribution to the mushroom
flora from twincity: Rawalpind & Islamabad
Khalid Rahman, Mushtaq Ahmad, Mir Ajab khan and Muhummad Ibrar Shinwari Ethnobotanical evaluation of medicinal plants of sabirabad and allied peripheries district Karak, Pakistan
Liaqat Ali, Najeeb ur Rehman, Ahmed Al-Harrasi, Ahmed Al-Rawahi, Javid Hussain and
Hidayat Hussain - New boswellic acid derivatives from the omani frankincense (Boswellia sacra)
Liaqat Ali, Najeeb-ur-Rehman, Ahmed Al-Harrasi, Ahmed Al-Rawahi, Rashid Al-Harrasi,
Javid Hussain and Hidayat Hussain - Nutritional assessment and the composition of essential
oils obtained from different grades of Omani Frankincense resin and leaves from Boswellia sacra
Malka Saba and Abdul Nasir Khalid - New Records of Uredinales (Basidiomycota) from Azad
Jammu & Kashmir and adjacent northern areas of Pakistan
Manzoor Hussain, Muqarrab Shah and Sadaf kayani - Palynological characterization of
certain Papilionaceous trees and shrubs of Kaghan valley Mansehra Pakistan
Maryum Ibrar Shinwari, Mir Ajab Khan and Muhammad Ibrar Shinwari Nomenclatural ambiguity in traded medicinal plants in Pakistan: Case-I, Gul-e-Gaozaban
Masood Jan, Farhatullah, Gul Jan and Farzana Gul Jan. Genetic diversity in indigenous
soybean germplasm and its us ancestral lines using morphological and molecular markers
Misbah Manzoor, Mufakhira Jan Durrani, Rukhsana Jabbeen, Shazia Irfan and
Farkhanda - Medicinal folk remedies of vegetables
Muhammad Ikram, Nausheen, M.Nisar, Sumaira Shaheen, Gul Jan and Farzana Gul Jan.
Genetic diversity in Pumpkin genotype: First report from Pakistan
Muhammad Ishtiaq, Mehvish Maqbool and Tanveer Hussain - Traditional culture and natural
resource management: A case study of bokarwal tribe in Tehsil Samahni (AK)
Mustajab Ahmed, Zafar Jamal, Shahid Nawaz, Ghulam Mujtaba Shah, Mushtaq Ahmad and

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Muhammad Zafar - Ethnobotanical studies on plant resources of Kukmong, District Abbottabad
PPTE- 30 Nabila Shah Jillani, Syeda Saleha Hassaney and M.T. Rajput - Vegetation of Rani Kot Fort
aera: A historical heritage of Sindh, Pakistan
PPTE- 31 Najeeb ur Rehman, Liaqat Ali, Ahmed Al-Harrasi, Ahmed Al-Rawahi, Rashid Al-Harrasi,
Javid Hussain and Hidayat Hussain - Evaluation of physico-chemical characteristics and GCMS profile of fruits and seeds of omani date palm (Phoenix dactylifera L.)
PPTE- 32 Najeeb Ur Rehman, Liaqat Ali, Hidayat Hussain, Javid Hussain and Ahmed Al-Harrasi Phytopharmacology of Nepeta clarkei
PPTE- 33 Naveed Alam, Mushtaq Ahmad, Muhammad Zaffar, Zahid Ullah, Kashmala Syed
- Ethnobotanical profile of 100 indicator species in tropical dry deciduous forests of Pakistan
PPTE- 34 Nisar Ahmad, Hina Fazal and Bilal Haider Abbasi - Medicinal plants profile of Madyan
Valley, District, Swat, Pakistan
PPTE- 35 Pirzada J.A. Siddiqui, Zaib-un-Nisa Burhan, Seema Shafique, Pervaiz Iqbal, Amjad Ali and
Shabir Ali Amir - Preliminary record of phytoplankton diversity and abundance along
Balochistan coast
PPTE- 36 Rahmatullah Qureshi, Amna Bibi, Muhammad Arshad and Shahida Khalid - Medicago
Scutellata (L.) Mill.: A new record for Pakistan
PPTE- 37 Rizwana Nawaz, Abdul Nasir Khalid and Muhammad Hanif - The genus agaricus
(Basidiomycota; Agaricales), its Diversity and Phylogeny from Lahore
PPTE- 38 S. Niaz, U. Younis, R. Raja, S. Suleman and A.A. Dasti - An annotated checklist, identification
key and illustration of the vascular flora of sandy deserts of Punjab, Pakistan
PPTE- 39 S. Sarwar, A. N. Khalid and B. Dentinger - A new species of Suillus from coniferous forests of
Pakistan based on morpho-anatomical and molecular analysis.
PPTE- 40 Sadaf Kayani, Manzoor Hussain, Misabah Tariq, Mir Ajab Khan, Mushtaq Ahmad and
Muhammad Zafar - Morpho-palynological studies of certain plants belonging to the genus
solanum from Abbottabad, Pakistan
PPTE- 41 Sadia Sadiq, Sumera Iqbal and Khajista Jabeen - Antifungal activity of Berberis aristata DC.
Exudate (Rasaunt) collected from local market of Lahore, Pakistan
PPTE- 42 Safia Janjua and Maliha Shahid - Phytochemical analysis and determination of medicinal
potential of organic kitchen waste
PPTE- 43 Safia Janjua, Maliha Shahid and Zafar Iqbal. Documentation of floral diversity of Banjosa
game reserve of Azad Jammu and Kashmir.
PPTE- 44 Samia Akram, Khan Rass Masood and Riffat Jabeen - Palynological evidence of
pteridophytic plant communities in late permian (dzulfian) deposits, western salt range, Pakistan
PPTE- 45 Samiullah and Asghari Bano - Traditional ethnobotanical uses of halophytes collected from
Katlang Region District Mardan, Pakistan
PPTE- 46 Sarfraz Ahmad, Muhammad Islam, Nayyar Razzaq and Muhammad Aslam Gill Production potential of economically important medicinal and aromatic plants in Balochistan,
Pakistan
PPTE- 47 Shujaul M Khan, Sue Page, Habib Ahmad, Zahidullah, Hamayun Shaheen, Mushtaq
Ahmad and David Harper - Health-giving flora and ethnoecological knowledge in the western
Himalayas; the Naran valley Pakistan
PPTE- 48 Shumaila Tahir, Asghari Bano, Warda Shahid, M. Aqeel Kamran, Amna, Rabya Mufti and
Hassan Javed Chaudhary - Ethno medicinal and pharmacological investigation of Araucaria
columnaris Hook., and Cycas revoluta Thunb.
PPTE- 49 Sofia Khalid and Sumaira Shan - Ethnobotanical study of plant resources in the Looni Village,
Tehsil Kallar Syedan, District Rawalpindi, Pakistan
PPTE- 50 Syeda Bint-e- Zahira and A.N. Khalid - New records of Trichoderma species from Pakistan,
identification based upon its-rDNA sequencing
PPTE- 51 Syeda Huma Bokhari, Barkat Ali, Mushtaq Ahmad, Muhammad Zafar, Iftikhar H. Shah
and Mir Ajab Khan - Palynomorphological studies of selected medicinal plants from Haripur,
Khyber Pakhtunkhwa, Pakistan
PPTE-52 Tayiba Ashraf and Abdul Nasir Khalid - Diversity and phylogeny of Helvella (Ascomycota:
Pezizales) from Himalayan moist temperate forests of Pakistan
PPTE-53 Zahid Ullah, Mushtaq Ahmad, Muhammad Ilyas, Mir Ajab Khan, Muhammad Zafar and
Rasool Bakhsh Tareen - Phytodiversity, life form, communities and species distribution pattern
in mountain ecosystem of Miandam Pakistan
PPTE-54 Zeb-un-Nisa, Gul Jan, Mushtaq Ahmad, Farzana Gul Jan, Rafiullah Khan and Muhammad
Zafar. Study of morphology, anatomy and classification of stomata in selected weeds

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PPTE-55 Zerqa Nawaz, Kaleem Ullah Kakar Rizwana Aleem Qureshi and M. Ishtiaq - Draba
sherwalii (Brassicaceae), a new addition to flora of Pakistan
PPTE-56 Zia-ur-Rehman Mashwani, Mir Ajab Khan, Mushtaq Ahmad, Muhammad Zafar and
Muhammad Arshad - Anticancer activity of Geranium wallichianum (Geraniaceae) indigenous
to Western Himalayas, Pakistan

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Additional Abstracts for Oral Presentation

OPPP-38 Saiqa Razi, Zainab Saeed, Abida Bano, Erum Ashiq, Soniya Irshad, Zarqa Ayub, and
Shaista Kanwal - Impact of single and mixed species inoculations on the growth promotion of
economically important crops
OPBB 32 Madiha Tanveer, Hina-Ur-Razaq Qureshi, M. Qaiser Fatmi and Tayyaba Yasmin - In silico
prediction of regulatory elements and corresponding protein-dna interaction in plant promoters
OPPE 42 Mansoor Hameed, Muhammad Ashraf, Nargis Naz, Tahira Nawaz, Riffat Batool, M. Sajid
Aqeel Ahamd, Farooq Ahmad and Mumtaz Hussain - Anatomical adaptations of Cynodon
dactylon (L.) pers. from the salt range (Pakistan) to salinity stress. ii. leaf anatomy
OPTE 34 Khawaja Shafique Ahmad, Mansoor Hameed, Muhammad Sajid Aqeel Ahmad,
Muhammad Ashraf, Farooq Ahmad, Zabta Khan Shinwari, Sadaf Habib and Mumtaz
Hussain - Economic evaluation of some plant resources from Neelum Valley Azad Jammu &
Kashmir (AJ&K)

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Additional Abstracts for Poster Presentation

PPBB 89 Muhammad Javid Iqbal, Mehboob Ur Rahman and Amer Jamil - Marker trait association for
drought tolerance in hexaploid wheat (Triticum aestivum L.) germplasm
PPBB 90 Sara Shauket, Muhammad Hassan, Yasir Maqsood and Muhammad Javid Iqbal Proximate, mineral and fatty acid profile of linseed (Linum usitatissimum L.)
PPBB 91 Iram Zovia, Maria Mohyoudin, Muhammad Javid Iqbal and Amer Jamil - Isolation of actin
and gapdh partial genes from Nigella sativa & Capsicum annum

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AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

ORAL ABSTRACTS
OPBG 1

EFFECT OF NITROGEN FERTILIZER ON THE YIELD OF MUNGBEAN CULTIVARS

UNDER THE AGROCLIMATIC CONDITIONS OF QUETTA
ABDUL KABIR KHAN ACHAKZAI1* AND HABIBULLAH2
1

Department of Botany, University of Balochistan, Quetta, Pakistan.

2
Government Degree College, Mastung, Balochistan, Pakistan.
*
Corresponding authors e-mail: [email protected]

The aim of the study was to evaluate the yield response of mungbean [Vigna radiata (L.) Wilczek] cultivars
subjected to different levels of applied N fertilizer. To achieve the goal, an experiment was conducted in the
experimental field of Agricultural Research Institute (ARI), Quetta. The soil of the study area was basic in reaction, salt
free, medium textured having low organic matter & total N contents. Four diverse cultivars of mungbean viz., NM-92,
NM-98, M-1, and NCM-209 were grown in kharif season for two consecutive years i.e., 2007 and 2008. Six different
levels of N fertilizer were applied @ zero, 20, 40, 60, 80 and 100 kg ha-1. At the same time a constant dose of P2O5 and
K2O were also applied to each N level (except control i.e. zero). Urea fertilizer was used as a source of N, while TSP and
SOP as sources of P & K, respectively. The plot size for treatment and cultivar were kept as 22.8 and 2.40m2,
respectively. The plot was arranged in a randomized complete block design (RCBD). Fertilizer was kept in main plot and
cultivar as sub-plots. Results showed that except of pods plant-1 and pods length, all remaining yield and yield
components exhibited significant (p<0.05) response toward added fertilizer. A maximum yield plant-1 (18.99 g), yield
plot-1 (284.30 g) and yield ha-1 (1185 kg) were obtained in nitrogen fertilizer @ 40 kg ha-1. Results further showed that
cultivars also responded significantly in relation to receiving various levels of applied N fertilizers. A maximum pods
length (7.68 cm), 100 seed weight (6.30 g), yield plant-1 (14.25 g), yield plot-1 (214.3 g) and yield ha-1 (893.6 kg) were
obtained in NM-92 cultivar. Whereas, minimum values for the same attributes were recorded forNCM-209 cultivar,
respectively. Hence mungbean NM-98 and M-1 were found intermediate in response toward added fertilizer. The
correlation coefficient (r) studies exhibited that number of pods plant-1(r=0.351), pods length (r=0.255), yield plant-1
(r=0.189) and yield plot-1 (r=1.00) were significantly (p<0.05) and positively correlated with their grain yield (kg ha-1).
Thus based on correlation studies it can be revealed that cultivars under cultivation displayed a wide range of variation
for most of the mentioned growth traits and could be exploited in breeding program to enrich the mungbean genetic
affluence.
OPBG 2

CALLOGENESIS AND EMBRYOGENESIS IN ORCHID (DENDROBIUM SABIN H.)

UNDER DIFFERENT LEVELS OF GROWTH REGULATORS
AKBAR ALI BHATTI1, ADNAN YOUNIS1, ATIF RIAZ1, BILQUEES FATIMA1,
MANSOOR HAMEED2 AND USMAN TARIQ1
1

Institute of Horticultural Sciences, University of Agriculture, Faisalabad 38040

2
Department of Botany, University of Agriculture, Faisalabad 38040

Callogenesis and embryogenesis of orchid (Dendrobium sabin H.) was studied on MS media containing different
levels of NAA for callogenesis, BAP alone and in combinations with NAA use for embryogenesis. Maximum (100%)
success percentage was observed in MS medium supplemented with NAA (0.2 mg L-1) after 10 days of culture and
minimum (50%) success percentage was observed in control. Success percentage decreased with increase in time span
and 93% success was observed in (0.2 mg L-1) NAA after 40 days of culture. The embryoids obtained from induced calli
showed greatest embryo formation (92%) at the highest level of NAA + BAP (0.4 mg L-1 + 0.4 mg L-1) than control
(76%) while BAP alone comparatively gave better results than control. It is concluded that leaf disc explant of orchid
induced maximum calli on callusing media at (0.2 mg L-1) of NAA and induced calli showed maximum embryogenic
response on media supplemented with MS medium at the highest level of NAA + BAP and developed somatic
embryogenesis.

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

OPBG 3

QUANTITATIVE TRAIT LOCI MAPPING OF DROUGHT TOLERANCE AT

GERMINAITON STAGE IN BREAD WHEAT
ARMGHAN SHAHZAD1,2*, SAMI ASHRAF2, FARWA KARAMAT2, MUHAMMAD IQBAL1,2,
JALAL-UD-DIN2,3 AND GHULAM MUHAMMAD ALI1,2
1
2

Plant Biotechnology Program (NIGAB),National Agriculture Research Center, Park Road 45500 Islamabad.
Department of Plant Genomics and Biotechnology, PARC Institute of Advance Studies in Agriculture, National
Agricultural Research Center, Park Road 45500 Islamabad.
3
Stress Physiology Program, National Agricultural Research Center, Park Road 45500 Islamabad.
*Correspondence: [email protected],

Drought is a major yield limiting factor in many crop species. Wheat is a major crop and staple food in Pakistan.
Genetic linkage map construction based on linked DNA markers spanning whole wheat genome and subsequent QTL
mapping for drought tolerance can enhance breeders ability for effective selection. We used an F8 population (80 lines)
derived from the cross of OPATA x SH-349 for drought tolerance at germination stage under controlled conditions. The
drought was induced by 15% PEG nutrient solution in acid washed sand medium under controlled conditions (14/10 hrs
light and dark and 250C). During four weeks of growth, data for germination percentage, germination rate index, shoot
root lengths and dry weights were recorded. These data along with marker data were used for QTL mapping using QTL
Cartographer V2.5 software. Single marker QTL analysis showed that 14 SSR markers were linked to QTLs for five
traits in both drought and control condition. Using simple interval mapping and composite interval mapping, QTLs for
different traits of interest were mapped on two linkage groups.On linkage group 1, QTLs for root length, shoot length,
dry biomass and germination percentage were mapped under control and drought conditions. On linkage group 2, QTLs
for germination percentage and germination rate were mapped under controlled and drought conditions. The population
will be screened with more SSR markers to increase the genomic coverage of this map.
OPBG 4

MANAGEMENT OF BLACK SCURF OF POTATO WITH EFFECTIVE MICROBES,

BIOLOGICAL POTASSIUM FERTILIZER (BPF) AND TRICHODERMA HARZIANUM
FARAH NAZ1*, C.A. RAUF1, I. AHMAD2, I.U. HAQUE1 AND A. RIAZ1
1

Department of Plant Pathology, PMAS-UAAR, Pakistan

Pakistan Agriculture Research Council, Islamabad Pakistan
Corresponding authors e-mail: [email protected]

The efficacy of soil application with microbial preparations viz. Trichoderma harzianum, effective microbe (EM)
culture and biological potassium fertilizer (BPF) was evaluated for the management of soil-borne inoculum of black scurf of
potato by sowing cv. Desiree. Soil application with three dosages of culture suspension of Trichoderma harzianum,
effective microbe (EM) culture and biological potassium fertilizer (BPF) were applied in the soil to know the efficacy of
these treatments in reducing the disease. Soil application with T. harzianum at the time of sowing followed by two and three
dosages at 20 days intervals gave significant protection to eyes with EGI of 30.55%, SK 24.07%, SCI 36.10%, StCI
30.60%, BSDI 26.43%, and YR of 35.09% against the fungus which ultimately contributed to better crop stand and
increased yield as compared to inoculated control and rest of the treatments. Soil application with T. harzianum gave
significant protection to eyes, sprouts and stolons, against soil-borne inoculum of the fungus which ultimately contributed to
better crop stand and increased yield as compared to inoculated control and rest of the treatments.
OPBG 5

LEAF RUST RESISTANCE IN SEMI DWARF WHEAT CULTIVARS: A CONSPECTUS

OF POST GREEN-REVOLUTION PERIOD IN PAKISTAN
GHULAM MUSTAFA*, MUHAMMAD MAQSOOD ALAM, SAMI ULLAH KHAN, MUHAMMAD NAVEED
AND ABDUL SAMAD MUMTAZ
Department of Plant Sciences, Quaid-i-Azam University, Islamabad Pakistan
*
Corresponding author: [email protected]

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

Leaf rust, the most prevalent biotic stress in wheat growing areas, remains a global concern for wheat production.
The pattern of leaf rust varies across countries and warrants assessing the pathogen and deployed resistant genes in
cultivars. This study evaluates the genetics of thirty-eight wheat varieties using molecular markers for six significant Lr
genes: Lr10, Lr13, Lr24, Lr26, Lr27 and Lr31 for the region. The analysis showed the presence of all six genes in only
four varieties. Due to minor differences in loci, the PCR based screening could lead to erroneous results. Therefore, a
novel ABI 3730 capillary array method was used with sensitivity of detecting as little as one nucleotide difference. This
not only has verified their successful application in varieties with diverse genetic background but has helped in reliably
detecting the presence of gene in commercial varieties. The STS markers (names) showed high efficiency verifying the
presence of four effective genes (Lr10, Lr21, Lr24 and Lr27) in our local cultivars with parallel analysis from field trial
at Regional Agriculture Institute, Bahawalpur, Pakistan. The data thus collated provided useful information in marker
assisted screening to integrate effective resistant genes. The data revealed wide distribution of Lr10, Lr13, Lr26, Lr27Lr31 and the absence of Lr21 and Lr24 in the Pakistani cultivars. Thus a clear gene pyramiding plan for wheat breeding
may be developed.
OPBG 6

FIELD EVALUATION OF THREE SAUDI ARABIAN DATE PALM VARIETIES (AJWA,

SAFAWI AND RUTHANA) AT KHAIRPUR, PAKISTAN
GHULAM SARWAR MARKHAND1, ADEL AHMED ABUL-SOAD2 AND MUSHTAQUE AHMED JATOI1
1

Date Palm Research institute, Shah Abdul Latif University, Khairpur, Pakistan.
2
Horticulture Research Institute, Agricultural Research Centre, Cairo, Egypt.
Corresponding authors e-mail: [email protected]

The most of the varieties of Khairpur are sensitive to monsoon rains which synchronize with the ripening season.
Urgent and rapid demand is being required for the high quality varieties resistant to monsoon rains. Offshoots of three
well known date palm varieties Ajwa, Safawi and Ruthana from Al-Madina, Kingdom of Saudi Arabia were brought and
cultivated in 2006 to study the adaptability of these varieties at the climatic conditions of Khairpur, Sindh, Pakistan. It is
worth to mention that there is no evidence for these three cultivars in Pakistan and particularly Ajwa is restricted to
climatic conditions of Al-Madina, Saudi Arabia and if established anywhere, variation occurred in the fruit size, texture
and quality. The Sites were selected after comprehensive study of official records to find common climatic conditions in
Khairpur and Al-Madina. The reading included minimum and maximum temperatures and relative humidity. An
appropriate Performa was designed to drop the scientific and commonly used properties such as: Fruit length, fruit
diameter, Flesh weight, stone weight, pH and Total Soluble Solids (TSS). New crop obtained in 2009. Previous data
were recorded for 3 sequential seasons (2009-11). The general features of date palm culture in Al-Madina, Saudi are not
much different from those in Khairpur, especially when climate, cultivation and utilization is concerned. It was
concluded from the study that the climatic conditions of Khairpur are very much suitable for planting of alien varieties of
dates like Ajwa, Safawi and Ruthana and other similar Saudi date palm varieties.
OPBG 7

IDENTIFICATION AND CHARACTERIZATION OF POST HARVEST FUNGAL

PATHOGENS OF MANGO FROM DOMESTIC MARKETS OF PUNJAB
HAMD MEER1, SHAZIA IRAM1, IFTIKHAR AHMAD2, FAISAL SOHAIL FATEH2
AND MUNAWAR RAZA KAZMI3
1

Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi.

PARC Institute of Advanced Studies in Agriculture, National Agricultural Research Center, Islamabad.
3
Programme Officer, ACIAR, Australian High Commission.

A systematic survey was conducted during October 2011 to assess the status of major post harvest diseases of
mango fruit in the major markets of Punjab. The data regarding prevalence, incidence and severity was collected and
then pathogen was isolated by tissue segment method. General and specific medias were used to isolate the fungi and
then eventually, frequency of each fungus was calculated media wise and location wise as well. The present study
indicated that anthracnose and stem end rot were 100% prevalent diseases of Punjab. Present study revealed that
anthracnose caused by Colletotrichum gleosporiodes, stem end rot caused by Lasiodiplodia theobromae, Alternaria rot
caused by Alternaria alternata and Aspergillus rot caused by Aspergillus niger and these were major post harvest
diseases that damage the mango fruit after harvesting. As far as, it was also observed that malt extract agar and V-8 agar
were the best medias for the growth of Lasiodiplodia theobromae and Colletotrichum gleosporiodes fungi. By present
investigation it would be possible to study the post harvest fungal pathogens of mango for their pathogenic behavior,
fungicides resistance and genetic variability and these all investigations will be very helpful for the management of postharvest diseases of mango.

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

OPBG 8

EFFECT OF PRUNING ON SEX EXPRESSION AND ITS RELATION WITH

PHYTOHORMONES CONTENT IN MONOECIOUS CUCUMBER
HIDAYATULLAH1*, ASGHARI BANO2 AND KHALID MAHMUD KHOKHAR1
1
Vegetable Crops Research Programme, Horticulture Research Institute, National Agricultural Research Center
Islamabad, Pakistan, 2Professor, Department of Biological Sciences, Quaid-e- Azam University Islamabad, Pakistan
* Corresponding authors e-mail address: [email protected]

The effect of mechanical stress on sex expression in cucumber cultivar Sialkot selection was examined. This
mechanical stress was created by pruning of lateral branches. Seeds were sown in the field and after reaching the
required number of lateral branches according to respective treatment, each new coming lateral branch were pinched off.
The greatest increase in female flower production, lowest to sex ratio, highest number of fruits/plant and yield were
recorded with the treatment T4 (main stem with 3 lateral branches) compared with control (without pruning). This is due
to decrease in endogenous GA level at blooming and fruiting stages and increase in IAA level at flower initiation,
blooming and fruiting stages. Yield increase in relation to control recorded up to 61 %.
OPBG 9

LINE TESTER ANALYSIS FOR GRAIN YIELD AND YIELD RELATED TRAITS IN
MAIZE VARIETY SARHAD WHITE
HIDAYAT UR RAHMAN1*, ASIF ALI1, ZAHIR SHAH2, M. IQBAL3 AND M. NOOR1
1

Deptt. of P.Breeding & Genetics, Agricultural University,Peshawar,Pakistan

2
Deptt. of Soil & Env. Sciences, Agricultural University,Peshawar,Pakistan
3
Cereal Crops Research Institute, Pirsabak, Nowshera, Pakistan
Corresponding authors e-mail: [email protected]

This study was conducted to evaluate the performance of testcross hybrids for grain yield and other morphological
traits. The objectives were to determine the magnitude of heterosis, combining ability, nature of gene action for ten
quantitative traits in testcross hybrids of maize variety Sarhad white. The experiment was conducted to generate 42
testcross hybrids from 14 S2 lines, three testers viz., Jalal, Kiramat and WD2. These along with five commercial checks
viz., Azam, 3025, 30P45, 30K08 and Opener were evaluated in partially balanced lattice square design with two
replications at experimental farm Khyber Pakhtunkhwa Agricultural University, Peshawar, Pakistan, during 2010-2011
in two seasons i.e. in spring (February - June) and summer (July - October). Data were recorded on days to tasseling,
silking and pollen shedding, anthesis silking interval (ASI), plant height, ear height, ear length, number of kernel rows
per ear,100 grain weight and grain yield. Results revealed the existence of highly significant differences among testcross
hybrids for all parameters except kernel rows per ear, which was non significant. Lines effect was significant for plant
height, non significant for the traits viz., days to tasseling, pollen shedding, cob length, kernel rows per ear, while rest of
the traits exhibited highly significant variations among female (Lines). Testers and interaction effect was highly
significant for all the traits under study except ASI and kernel rows per ear, which were non significant for testers.
However, highly significant variations were observed for all the traits except kernel rows per ear. Testcross hybrids
expressed significant variability for 10 quantitative characters studied. The study on heterosis for grain yield revealed
that 14 testcross hybrids showed significantly positive and 28 testcross hybrids showed significantly negative mid parent,
while 6 testcross hybrids expressed significantly positive and 36 testcross hybrids expressed significantly negative better
parent heterosis. For grain yield significant standard heterosis in positive direction was observed for 8 testcross hybrids
over the check Azam, for 2 testcross hybrids over 30P45, for 30 testcross hybrids over 3025, for 29 testcross hybrids
over 30K08 and for 8 testcross hybrids over the commercial check Opener. It is suggested to evaluate the identified
testcross hybrids in large scale trial to confirm their superiority. The study on the combining ability revealed that the
lines viz., SWAJK 1-4 , SWAJK 3-1 , SWAJK 3-3, SWAJK 3-4 , SWAJK 4-3 and SWAJK 4-4 revealed significant
GCA effects and the tester WD2 was found to be best general combiners than the rest. Regarding SCA effects, 12
testcross hybrids showed significant positive SCA effects for grain yield. Promising hybrids involving high high
parental GCA effects for grain yield may be used for further improvement of lines by selection in advanced generations.
OPBG 10

MOLECULAR CHARACTERIZATION OF OLIVE GERMPLASM USING DNA MARKERS

IHSAN ULLAH1*, SYEDA MAARIYAH HASNI2, MUHAMMAD ZAFFAR IQBAL1,
MUHAMMAD NAWAZ2 AND SHAKRA JAMIL1
1

Agricultural Biotechnology Research Institute, AARI, Faisalabad; 2Govt. College University, Faisalabad
*
Correspondening authors e-mail: [email protected]

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

Olive (Olea europea L.) is priced for its premium oil quality as well as table use. Extensive natural plantation of wild
species of olive exists in several parts of Pakistan particularly in Khyber-Pakhtoonkhwa and Balochistan indicating possibility
of successful plantation of cultivated olives. However, endemic olive accessions found in Pakistan are uncharacterized,
hindering any systematic plan to propagate and promote commercial cultivation. Hence the present study was conducted at
Agricultural Biotechnology Research Institute, Faisalabad in context to characterize a set of olive germplasm by using DNA
markers. A total of 32 olive varieties/genotypes were subjected to 100 Random Amplified Polymorphic DNA (RAPD) markers
for molecular characterization through determination of genetic distance and relatedness among olive genotypes and
construction of a dendrogram for assessment of their phylogenic relationships. The eightyinformative DNA markers identified
through RAPD profiling varied in size from 290 to 2150 bp. The resulting similarity matrix revealed a high degree of mean
genetic diversity of 29% among the genotypes studied. The genotypes Hamdi and Nocellara emerged as most similar with
value of 96%. The genotypes Mavrino and HP-Olive were found most dissimilar with dissimilarity coefficient of 50%. Cluster
analysis using NTSYspc 2.2 software groped 32 genotypes into sevenmain clusters i.e. A, B, C, D, E, F and G. It was
concluded that a considerable genetic diversity exists among Pakistani olives. The information generated here will be useful for
characterization of olive germplasm and finding variety specific markers.
OPBG 11

ANALYSIS OF SOIL MICROBIAL BIOMASS DYNAMICS IN RAINFED WHEAT

FIELDS IN ARID ZONE OF PAKISTAN
ISRAR ASGHAR1, MUHAMMAD AKMAL1, MUHAMMAD ISHTIAQ CH2* MEHWISH MAQBOOL2,
KHALID SAIFULLAH KHAN1 AND GHULAM SHABBIR1
1

Department of Agriculture Research, Pir Mehr Ali Shah University Rawalpindi, (UAAR) Pakistan
Department of Botany, Mirpur University of Science and Technology, Bhimber, Azad Kashmir, Pakistan
*Corresponding author: [email protected]

In order to investigate Soil Microbial Biomass (SMB) dynamics in rainfed wheat fields, a field (model) experiment
was conducted in research farm of Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi in 2010. Wheat (Triticum
asitiuam, Chakwal-50) was grown under three treatments of recommended doses of nitrogen and phosphorous (NP) as
control (CK); no other fertilizer was used. In study half recommended dose of NP ( NP) at the rate of 40:20 kg NP ha-1
and full recommended dose of NP (NP) at the rate of 80:40 kg NP ha-1 was applied. A composite soil sample was taken
from the depth of 0-15 cm from each treatment, every month throughout the growing period of crop. Each time soil
microbial biomass parameters as soil microbial biomass C, N, P,C:N and C:P (Cmic, Nmic, Pmic, Cmic: Nmic and Cmic: Pmic)
were determined by using fumigation extraction method and the results were co-related with the growth stages of wheat ,
climatic parameters (Air temperature, Soil temperature, Rainfall and Soil moisture content) and the effect of treatments.
the results indicated that Cmic, Cmic: Nmic and Cmic: Pmic were highest at the early stage of wheat crop then declined in the
middle and lowest at the crop maturity while Nmic and Pmic were low in the initial growth period of wheat crop then
reached to their peak point in middle and were lowest at the stage of maturity. All indices of SMB were increased by
providing a balance fertilizer which was cleared from the results, in CK the total amount of Cmic, Nmic, Pmic, Cmic: Nmic
and Cmic: Pmic during whole wheat growth was 861.08 mg kg-1, 84 mg kg-1, 31.91 mg kg-1,83.81 mg kg-1and 224.19 mg
kg-1 while in NP it was1260.4 mg kg-1,104.07 mg kg-1,45.69 mg kg-1,98.35 mg kg-1 and 230.33 mg kg-1 and the values
of these parameters in NP was 1435.42 mg kg-1,112.68 mg kg-1,59.65 mg kg-1,102.78 mg kg-1 and 198.5 mg kg-1,
respectively. Seasonal variation was more visible in this study. The climatic data indicated that there was extreme
drought as in initial four months (October, November, December and January). The average rainfall was only 8.2 mm
while in next four months (February, March, April and May) it was higher as compared to initial period the average
rainfall during this period was 70 mm during this period .The average values of Cmic (155.79 mg kg-1), Cmic: Nmic (12.72
mg kg-1) and Cmic: Pmic (28.57 mg kg-1) were high during drought period while Nmic (12.33 mg kg-1)and Pmic (5.65 mg kg1
) were low during this period as compared to rainy season. SMB was also fluctuated by temperature, both air and soil
temperature was involved in this variation. Cmic, Pmic and Cmic: Nmic were high in winter when the temperature was much
low as in January the average temperature was 12oC and was high in spring while Nmic and Cmic: Pmic were high in
summer when the average temperature of May was 29oC.The fluctuation in pH was also observed in this study but it was
fluctuated up to 8 in the whole experiment even under treatments as well as seasonal changes. The considerable seasonal
changes and variation due to fertilization in SMB indicated the direct relationship of nutrient and season parameters with
overall SMB and its paramount impact on growth and yield of crop in arid zones of Pakistan.
OPBG 12

TISSUE CULTURE RESPONSES OF SOME WHEAT (TRITICUM AESTIVUM L.)

CULTIVARS GROWN IN PAKISTAN
KHALID MEHMOOD1, MUHAMMAD ARSHAD1*, GHULAM MUHAMMAD ALI2, RAHMATULLAH
QURESHI1 AND ABDUL RAZZAQ3
1

Department of Botany, Pir Mehr Ali Shah, Arid Agriculture University Rawalpindi, Pakistan

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

6
2

Plant Biotechnology, NIGAB, National Agriculture Research Center Park Road Islamabad, Pakistan
3
Department of Agronomy, Pir Mehr Ali Shah, Arid Agriculture University Rawalpindi, Pakistan
*Corresponding author. E-mail: [email protected].

Good tissue culture response for callus induction and regeneration is prerequisite for improvement of wheat through
genetic transformation. Tissue culture response of six wheat cultivars was studied using MS and N6 medium supplemented
with different concentration of 2,4-D (2,4-Dichlorophenoxyacetic acid) and BAP (6-benzylaminopurine) for callus induction
and regeneration, respectively. Mature seeds were used as explants. All cultivars exhibited best response for callus induction
and regeneration on MS medium as compared to N6 medium. However, significant differences among cultivars were
observed. Each cultivar responded differently at different levels of growth regulator for callus induction. Inqalab-91 and
Lasani-08 showed maximum callus induction (90 %) and (78.78 %), respectively at 3mg/l of 2, 4-D. Tatara showed 84.43 %
callus at 2.0mg/l, chakwal-97 77.08 % at 2.5 mg/l while GA-02 and Khyber showed 74.30 % and 65.97 % callus induction
response, respectively, at 3.5 mg/l of 2, 4-D. As regards regeneration, direct shoots and roots development were observed by
using different concentration of BAP. Significantly higher regeneration (59.33%) was observed in Chakwal-97 with 3.0 mg/l of
BAP while least regeneration was observed in Khyber (17.33%) at 4.0 mg/l among all cultivars. It was also observed that all
cultivars showed shoot as well as root development with 3 and 5mg/l of BAP. Using 8mg/l agar rather than 6 and 10 mg/l
significantly enhanced regeneration ability of cultivars. The results of present findings will be helpful for selecting the most
tissue culture responsive cultivars for genetic transformation against different biotic and abiotic stresses as well as for
improvement of important agronomic traits of wheat crop.
OPBG 13

GLU-DT1 ALLELIC VARIATION IN SYNTHETIC HEXAPLOID WHEATS DERIVED FROM

DURUM CULTIVAR DECOY AEGILOPS TAUSCHII ACCESSIONAL CROSSES
MADEEHA KHALID1, TARIQ MAHMOOD1, AWAIS RASHEED1, ALVINA GUL KAZI2
AND ABDUL MUJEEB-KAZI3*
1

Plant Biochemistry and Molecular Biology Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad,
Pakistan; 2Atta-ur-Rehman School of Applied Biosciences (ASAB), National University of Science and Technology
(NUST), Islamabad, Pakistan; 3Wheat Wide Crosses and Cytogenetic Program, National Institute for Biotechnology and
Genetic Engineering (NIBGE), Faisalabad, Pakistan
Corresponding author: [email protected]
Characterization of high molecular weight glutenin subunits is the fundamental approach for categorizing genotypes
with good bread making quality. Allelic variation at Glu-Dt1 locus is major determinant of bread wheat end use quality.
In synthetic hexaploid wheats (SHWs), the D-genome encodes numerous allelic variants of high molecular weight
glutenin subunits that require appropriate identification prior to their exploitation for bread wheat improvement. This
study was conducted to identify allelic variation at Glu-Dt1 locus of 47 accessions of D-genome synthetic wheats derived
from the crossing of durum cultivar Decoy with different accessions of Aegilops tauschii. Biochemical (SDS-PAGE)
and molecular marker techniques were used to stringently characterize allelic differentiation. Ten different alleles at GluDt1 locus were observed which formed 16 different subunit combinations. The frequency of inferior quality encoding
allele, 1Dx2+1Dy12, was low (19.14%) as compared to the frequency of superior quality encoding allele, 1Dx5+1Dy10
(21.27%). Additional validation was carried out with co-dominant molecular markers for Glu-A1c (Null), Glu-D1d
(1Dx5+1Dy10), Glu-D1a (1Dx2+1Dy12) and Glu-D1-1g (1Dx2.1) alleles. The high number of glutenin subunits
observed in SHWs are suggestive that somewhat narrow genetic base for D-genome encoded glutenin subunits in bread
wheat may be broadened by exploiting this diploid genomic grass resource through targeting allelic transfers from the
synthetic hexaploid genetic stocks. The identification of these new allelic combination resources in SHWs also provide
an option replacing other inferior alleles within the D-genome within elite cultivars by better allelic variants that have
become available at the Glu-Dt1 locus of SHWs being inherited from diverse Ae. tauschii accessions.
OPBG 14

EFFECT OF POLLEN SOURCES AND PLOIDY LEVELS ON DOUBLED

HAPLOID PRODUCTION IN WHEAT
MAKHDOOM HUSSAIN1, MUBASHIR NIAZ3*, MUHAMMAD IQBAL3, JAVED AHMAD1, MUHAMMAD
ASIF2, G.M. SUBHANI1, SAJID-UR-RAHMAN2 AND TEHREEMA IFTIKHAR3
1
Wheat Research Institute, Faisalabad, Pakistan,
Agri. Biotechnology Research Institute, Faisalabad, Pakistan
3
Laboratory of Mycology & Biotechnology, Department of Botany, Government College University, Faisalabad
*
Corresponding Author: [email protected]
2

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

Field experiments carried out to analyse the efficiency of pollen parents reflected that embryo formation was
significantly affected by maize pollen sources in wheat x maize crossing. Crosses among selected pollen sources, FSH399, Sultan and 73Q3 were found as efficient pollen parents that yielded higher embryo formation (28.0%) when
crossed with wheat. Various pollen sources were evaluated for the efficiency of embryo formation. The results expressed
that crosses between wheat F1MH-97 x Auqab-2000 and the maize cultivar Sadaf yielded on average 37.67%
embryos/pollinated florets, so Sadaf was identified as best pollen source to use in wheat x maize crossing.Results of
crosses between the best pollen sources and various female wheat parents of different ploidy levels (hexaploid and
tetraploid) indicated that hexaploid wheat perform better than tetraploid in crosses with maize. However, successful
production of embryos at a relatively high frequency could be achieved in tetraploidwheats with FSH-399. The highest
frequency of seed set was produced from a cross between the hexaploid wheat Cross MH-97 x Auqab-2000and Neelum.
OPBG 15

DEVELOPING PROTOCOL FOR GENETIC TRANSFORMATION OF GRAPES

(VITIS VINEFERA) KING, S RUBY C.V
MAQSOOD AHMED1*, HAFIZ ASHFAQ AHMED3, RAHMATULLAH QURESHI2,
NADRA KHAN1, R. MOHIB MUAZZAM1 AND M. RIAZ KHAN1
1

Department of Horticulture, Faculty of Agriculture, The University of Poonch- Rawalakot (AJK)

2
Deparment of Botany, PMAS Arid Agriculture University, Rawalpindi-Pakistan
3
Department of Horticulture, PMAS Arid Agriculture University, Rawalpindi-Pakistan
*
Corresponding authors e-mail: [email protected]

The grape (Vitis vinifera L.) belongs to the family Vitaceae, is commercially grown in the world. This crop is seriously
damaged by fungal diseases and growers are being faced extensive losses in yield and quality annually. This problem can be
overcome by breeding and by application of chemical but unfortunately, both struggles still ineffective due to long time
process of breeding and chemical / fungicides may have hazardous health effect and undesirable environmental risk.
Method of genetic transformation enhances defense mechanism of plant against disease. The present study was therefore,
carried out to develop protocol for genetic transformation of King,s Ruby variety of grapes through Agrobacterium
tumefaciens. The chitinase for (fungal resistance) and GUS gene (for phenotypic expression of transgenes) was introduced
in callus, developed from in vitro leaves through Agrobacterium strain (LBA4404) harbouring plasmid pBI121 nptII as
selectable marker gene in GUS and hptII for chitinase gene.For tissue culture and transformation parameters, in vitro shoot
tips were established and significantly higher number (5.33) and length of shoots (2.7 cm) was recorded when half strength
MS media was supplemented with BAP and GA3 @ 1 mg-l and 0.1 uM-l. The highest callus induction rate (73.0%) was
obtained on media having 2-4-D+ BAP+NAA at the rate of 2.0mg-l, 0.3mg-l and0.2 mg-l. Concerning regeneration rate,
maximum was 53.33% as a result of treatment combination NAA and BAP with concentration of 0.5 mg-l + 1.5 mg-l.
Regarding transformation efficiency rate, ten minutes inoculation period with bacterial suspension showed the highest
transformation efficiency rate 2.83 with chitinase gene but with Gus gene maximum transformation efficiency was 2.5.
Infected calli with chitinase gene and Gus gene co-cultivated for 2 days showed the maximum transformation efficiency
2.75 and 3.25. For elimination of excess bacteria, cefotaxime sodium treatment (300 mg-l) showed the highest survival rate
3.16 for chitinase gene and 2.5 for calli treated with Gus gene. Among washing duration treatments, 45 minutes washing
have shown best results in calluses treated with chitinase gene (3.08) and Gus gene (3.08) than remaining all other
treatments. The maximum i.e.2.83 transformation efficiency rate was achieved at 10 mg-l of hygromycin for selection of
transformed calli. Highest (2.25) transformation efficiency and survival rate was yielded when kanmycin was used`at the
rate of 100 mg-l. Five out of 8 calli showed positive expression and 62.5 % transformation efficiency through histochemical
GUS assay. The presenceand stable integration of transgenes in transgenic calli was confirmed by PCR in case of infection
with chitinase gene. Present results navigated that optimization for genetic transformation was simple and efficient
technique, could be helpful for further genetic improvement against fungal disease, reduce the demand of fungicides and
also overcome the hazardous heath and environmental risks.
OPBG 16

THERAPEUTIC HORTICULTURE: INFLUENCING PSYCHOLOGICAL RESPONSES OF

SURGICAL PATIENTS AND THEIR ENVIRONMENTAL ASSESSMENT SCALE
MUHAMMAD ALI KHAN1,2*, NOOR UL AMIN2, MUHAMMAD SAJID2, ASIF SHAH1
AND MUHAMMAD REZAUL KABIR1
1

Department of Ornamental Horticulture and Landscape Architecture, China Agricultural

University, Haidian District, Beijing 100193, P R China
2
Department of Horticulture, Khyber Pukhtookhwa Agricultural University, Peshawar, Pakistan
*
Corresponding Author: [email protected]

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

Therapeutic impact of foliage plants and flower arrangements were evaluated on the post operative recovery of
surgical patients in hospital wards. Two hundred and seventy surgical patients were randomly assigned to hospital wards
with plants and without plants representing ward A and ward B, respectively, making a lot of one hundred and thirty five
patients in each comparing ward. Eight species of flowers and foliage plants were placed in ward A. Various methods
like Patients questionnaire, small group discussion with ward nurses and focal interviews with ward doctors were used
for collecting patients data. Data collected includes patients physiological status evaluating traits viz., ratings of
patient's anxiety, fatigue, pain intensity and patients social behavior, Environmental Assessment Scale, Patients
personal preferences for plants and flowers and intake of postoperative analgesics. Statistically analysis of data was done
through Statistical Package for Social Studies (SPSS) by using chi-square test. More effective health signs were observed
for patients admitted in the ward A than those in the ward B. Patients admitted in ward A had significantly fewer
consumption of postoperative analgesic because of exhibiting more controlled and normal psychological and cognitive
responses. Patients stays in plants and flowers experienced less intensity of pain, anxiety, fatigue, elevated mood and
were socially more active and friendly towards other patients and nursing staff, expressing more positive feelings and
satisfying comments about their ward when compared with patients in the control group i-e., ward B. A more calm,
soothing and pleasing ward environment was described by patients stayed in ward with plants and flowers in comparison
to patients in no plants ward. Additionally most of the patients in ward A preferred Cut flowers, especially red color
roses over the green and variegated foliage plants. Furthermore, Small group discussion and focal interviews with nurses
and doctors affirms that arrangement plants and flowers in hospitals is an effective, inexpensive and more productive
complementary approach in medical field for surgical patients. These findings confirm and approve the application of
therapeutic horticultural approach for patients in hospitals.
OPBG 17

FORAGE YIELD AS AFFECTED BY COMMON VETCH IN DIFFERENT SEEDING

RATIOS WITH WINTER CEREALS IN POTHOWAR REGION OF PAKISTAN
MUHAMMAD ASAD MUKHTAR1, MUHAMMAD ANSAR1*, SHAHZADA SOHAIL IJAZ2,
MUHAMMAD AZEEM MALIK1 AND GHULAM SHABBIR3
1

Department of Agronomy, PMAS-Arid Agriculture University Rawalpindi

Department of Soil Science, PMAS-Arid Agriculture University Rawalpindi
3
Department of Plant Breeding and Genetics, PMAS-Arid Agriculture University Rawalpindi
*Corresponding authors e-mail: [email protected]
2

In rainfed area of Pothowar, there is little trend to inter or mix crop the cereals with legumes for production of
nutritious forage. The main problem for raising livestock in these areas is the provision of green fodder for animal during
winter months. The livestock community has no option except to feed wheat straw and summer cereal stalks to their
livestock which are nutritionally not rich source of feed for animals. Therefore, an experiment was conducted to evaluate
the forage yield of three winter cereals viz: oat, wheat and barley by mixing with vetch legume in three different ratios as
87:13, 75:25, 50:50 in addition to establish pure stands of cereals as well as vetch to find out the best yielding seeding
combination. The experiment was conducted at University Research Farm (URF) Chakwal Road PMAS-Arid
Agriculture University Rawalpindi during winter 2010 -2011. From the results it was concluded that cereal - vetch
combination in 75:25 seeding ratio performed better in terms of green as well as dry matter yield production irrespective
of mixed cereal crop. Land equivalent ratio showed that oat-vetch, barley vetch and wheat vetch 75:25 seeding ration
resulted 1.06, 1.04 and 1.10 LER values which clearly indicates the advantage of cereal-vetch combinations over pure
stands. Similarly, oat-vetch, barley- vetch and wheat-vetch in 75:25 seeding ration resulted 6.16%, 4.8% and 10.00%
higher water use efficiency (WUE) and an average 16.28 %, 16.80% and 23.90% higher photosynthetic active radiation
(PAR) values than their respective pure stands indicating the efficient utilization of natural resources. The wheat-vetch,
oat-vetch and barley 75:25 seeding ratio produced 7 %, 4% and 5% respectively higher green forage yield than their
respective cereal crops sown in pure stand. The number of tillers and leaves per plant increased with the increase of
seeding proportion of vetch plants in the respective mixtures clearly indicating the beneficial effect of cereal legume
mixture on forage yield.
OPBG 18

VIRULENCE PATTERN OF STRIPE RUST (PUCCINIA STRIIFORMIS) IN PAKISTAN

MUHAMMAD FAYYAZ, ATIQ-UR-REHMAN RATTU, SHAHZAD ASAD, MUHAMMAD IQBAL*, KHALIL
AHMED KHANZADA, MUHAMMAD ZAKRIA, JAVED IQBAL MIRZA AND SHAMIM IFTIKHAR
Crop Diseases Research Programme, IPEP, NARC, Islamabad
*
Plant Biotechnology Programme, NARC, Islamabad

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

Wheat stripe rust (Yellow rust disease) caused by fungus Puccinia striiformis f.sp. tritici is the major problem of
wheat production in the most parts of Pakistan and appears every year in the country. Monitoring of the pathogen
virulence factors and their changes provides basic information for the development of an early warning system to
breeders and researchers. To monitor the yearly regular virulence changes of yellow rust, a nursery specially designed
comprised of yellow rust differentials received from ICARDA and released Pakistani commercial wheat varities were
planted at hot spots of yellow rust for the five consecutive years 2005-2010. Upon the appearance of high infection and
severity under natural infection on susceptible variety Morocco (universal check) the response of each line was assessed.
In five year studies, the lines showed the similar behavior at all the tested location. Results revealed that no virulence
was observed on yellow rust resistance genes Yr3,Yr5, Yr10, Yr15, YrSP, and YrCVat all locations . Virulence on genes
for yellow rust resistance Yr1, Yr2, Yr6, Yr7, Yr8, Yr9, Yr17, Yr18, Yr24,Yr26, Yr27, Yr28, Yr29 and Yr31 was common
during 5 years of study at almost all locations. Some isogenic lines in different genetic back ground responded
differently. Wheat commercial varieties showed erratic pattern. Seher-2006 leading wheat variety was found resistant to
yellow rust at all tested locations. Newly released wheat varieties Faislabad-2008, Lasni-08, Aas-2010 , Hashim-08,
Janbaz, Ghaznavi, BARS-2009, NIFA-Barsta10, NARC-2009 and AARi-2010 were found effective against the
prevailing races of yellow rust. Year 2007-08 was totally escaped for yellow rust in the Pakistan.
OPBG 19

MOLECULAR GENETIC VARIATION FOR STRIPE RUST RESISTANCE IN

PAKISTANI SPRING WHEAT
MUHAMMAD IQBAL1,2*, MAHWISH EJAZ1,2, MAHRUKH BIBI3, IFTIKHAR AHMED1,2,
ARMGHAN SHAHZAD1,2 AND GHULAM M. ALI1,2
1

National Institute for Genomics and Advance Biotechnology, NARC, Islamabad

Department of Plant Genomics & Biotechnology, PARC, National Agricultural Research Centre, Islamabad
3
Department of Plant Breeding & Genetics, PMAS Arid Agriculture University, Rawalpindi

Stripe rust caused by Puccinia striiformis f. sp. tritici is one of the major biotic constraints to wheat production
throughout the world. Stripe rust can be effectively controlled by developing resistant wheat varieties. This, however,
requires identification of resistant sources to be used as parents in breeding programs. Molecular markers provide a quick
way of detecting rust resistance genes in adapted wheat material. The present study was conducted to detect stripe rust
resistance genes in 60 Pakistani adapted spring wheat varieties using 11 pairs of microsatellite and sequence tagged site
markers. In this study, maximum frequency of stripe rust resistance gene was observed for Yr26 (72-80%) followed by
Yr5 (45%), Yr10 (30%), Yr9 (25%), Yr18 (8-15%) and Yr17 (11%), respectively. Results of this study can help wheat
breeders in pyramiding rust resistance genes in future wheat varieties using Marker Assisted Selection.
OPBG 20

NUMBER OF GENES AND THEIR EFFECTS CONTROLLING GRAIN FILLING

DURATION IN TWO WHEAT (TRITICUM AESTIVUM L.) CROSSES
MUHAMMAD IRFAQ1*, MIR AJAB KHAN2, GUL SANAT SHAH KHATTAK1, ABDUL JABBAR KHAN,
TILA MOHAMMAD1 AND FAZLE SUBHAN
1

Plant Breeding and genetics Division, Institute for Food and Agriculture (NIFA), Peshawar, Pakistan.
2
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
*
Corresponding authors e-mail: [email protected]

Genetic effects for grain filling duration (GFD) were determined in two bread wheat combinations (B-92 x
Frontana: Cross 1; Inqilab-91 x FS: Cross 2) during 2006-07 and 2008-09. The genetic effects were determined by using
joint segregation analysis (JSA) as statistical procedure designed for six basic populations P1, F1, P2, B1, B2, and F2. The
results indicated that the trait in the crosses was controlled by mixed action of two major genes along with polygene with
their additive and dominant effects. Negative additive effects were found due to both first and second major genes in the
crosses in both years indicating that the major genes may affect the trait adversely except cross 1 during first year where
the additive effect was positive due to the second major gene. However, positive additive effects due to polygene were
observed in the crosses during both seasons. Transgressive segregates for both long and short GFD indicated the
dispersion of favorable and adverse genes in the parents. Major genes heritability for the trait was higher compared to
that of the polygene in B1, B2 and F2 for the crosses with highest environmental influence. Additive genetic effect of
major genes and polygene were pronounced thus selection of desirable recombinants for both short and long GFD may
be delayed up to advance generations such that maximum favorable genes are accumulated in the recombinants.

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

10

OPBG 21

ACIDOVORAX AVENAE SUBSP. AVENAE FIRST TIME CAUSING RED

STRIPE OF SUGARCANE IN PAKISTAN
M. I. HAQUE 1* AND SYED ZIA-UL-HUSSNAIN2
1
2

Department of Plant Pathology, Pir Mehr Ali Shah (PMAS) Arid Agriculture University Rawalpindi, Pakistan
Department of Plant Pathology, Pir Mehr Ali Shah (PMAS) Arid Agriculture University Rawalpindi, Pakistan
*
Corresponding authors e-mail: [email protected]

Acidovorax avenae subsp. avenae is the causal agent of red stripe disease of sugarcane, an important new disease
appeared from the last two years on the sugarcane promising clones in Punjab Province, Pakistan. Watery green stripe near
the midrib of the leaf was observed on promising clones of sugarcane viz: NSG-49, CPSG-2453, CP-NIA-82-223,
CSSG-2402 and US-114 planted during autumn 2009-2011. Bacterial colonies were obtained from infected leaf of the
variety CSSG-2402 on yeast extract dextrose chalk agar (YDC) media. The Isolate were tested for gram reaction,
oxidase,urease production, citrate utilization and catalase activity. DNA was extracted and 1500bp 16S rRNA gene was
amplified by using universal primer. Amplified product has been purified and in the process of sequencing for the
identification of Acidovorax avenae subsp. Avenae.On the bases of morphological appearance, biochemical and
molecular characterizations bacteria was identified as Acidovorax avenae subsp. avenae. The strain was detected by direct
antibody coating Enzyme Linked Immunosorbent Assay (DAC-ELISA) using antiserum raised against Acidovorax
avenae subsp. avenae. 27 Clones were used for ELISA test at 405 nm. The bacterium suspension (5 107 CFU/ml) was
prepared from freshly grown pure culture of A. avenae subsp. Avenae forinoculation into the growing points. The
pathogen was re-isolated from the inoculated plants and identified as A. avenae subsp. avenae. To our knowledge this is
the first report of red strip of sugarcane in Punjab province of Pakistan.
OPBG 22

THE EFFECT OF PLANTING DATE, IRRIGATION AND NITROGEN ON SOME TRAITS

OF FORAGE MILLET (PENNISETUM AMERICANUM VAR. NUTRIFEED)
MOHAMMAD JAVAD SEGHATOLESLAMI1, GHOLAMREZA MOUSAVI2 AND MAHDI JAFARI3
1

Associated professor of Islamic Azad University, Birjand branch, Iran

2
Assistant professor of Islamic Azad University, Birjand branch, Iran
3
Former M.Sc. Student of Islamic Azad University, Birjand branch, Iran
In order to study the effect of irrigation, nitrogen and planting date on forage millet an experiment was conducted in
Nehbandan, Iran in 2010. A double split plot design based on randomized complete block with three replications was
used. Two planting dates (21th May and 9th June), two irrigation intervals (one and two weeks) and three nitrogen levels
(75, 150 and 225 kg ha-1) were as main plot, sub plot and sub sub plot, respectively. The results showed that planting
date, irrigation interval and nitrogen levels had a significant effect on the most measured traits. The second planting date
(9th June) reduced significantly plant height, stem diameter and leaf to stem ratio, but increased tiller number per plant
and biomass yield. Although every other week irrigation significantly declined plant height, stem diameter, tiller number
per plant, biomass yield and WUE, but increased leaf to stem ratio. Nitrogen application increased significantly all above
mentioned traits. Interaction of irrigation and nitrogen on WUE was significant. In weekly irrigation treatment (non
stress condition) application of 225 kg ha-1 N compared to 75 kg ha-1 N, increased WUE, but in every other week
irrigation treatment (water stress condition) high quantity nitrogen application decreased WUE. In conclusion the highest
biomass yield was obtained in the second planting date, weekly irrigation and application of 225 kg ha-1 nitrogen.
OPBG 23

WHEAT RESPONSE TO VARIOUS TILLAGE-HERBICIDE INTERACTIVE SYSTEMS

UNDER SEMI-ARID CLIMATE
M. RAMZAN ANSER2, FAISAL ZAHOOR1, MUHAMMAD AZIM MALIK2, KHALID MAHMOOD1,
MUSHTAQ HUSSAIN KAZMI1, MUHAMMAD RAHEED2 AND S. H. RAZA3
1

Department of Agronomy, Faculty of Agriculture, Rawalakot, University of Azad Jammu & Kashmir
2
Department of Agronomy, PMAS-Arid Agriculture University, Rawalpindi
3
Institute of Plant Nutrition, Olshausenstr 40-60 24098, CAU Kiel, Germany

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Rainfed wheat is the major stay of resource-poor farming communities to earn livelihood in drought and erosion hit
northern region of Punjab (Pothwar) where it is growth in rotation with summer fallow. Intensive tillage system
involving one deep ploughing with Moldboard plough at the onset of monsoon during summer following by 7-8 shallow
cultivations during the fallow period (till wheat sowing) has been regarded as one of the best management practice
(BMP) to control weeds and harvest rainwater in soil profile for succeeding wheat. However, the trends of farmers
shifting to conservation tillage in dryland areas with Eco-fallow management system lead us to conduct a field studies to
develop alternate, farmer friendly and sustainable tillage systems. This research evaluated the impact of various tillageherbicide interactive systems on insitu rainwater harvesting and wheat yield. The experiment integrated glyphosate as
eco-fallow management. The experiment was laid out in Randomized Complete Block Design with three replications and
net plot size of 14 m x 10 m. Wheat cultivar GA-2002 was planted as a test crop. The data showed the superiority of
conservation tillage in terms of conservation of moisture and increasing wheat grain yields. Results also elaborated that
tillage cannot be completely eliminated for profitable fallow management. However, deep ploughing with moldboard
followed by single application of glyphosate proved potential option for substituting shallow tillage carried out during
summer season. The additional benefits under this tillage system included saving in fuel, labour and lower depreciation
and maintenance costs for tillage machinery in addition to unquantifiable environmental benefits.
OPBG 24

IMPROVEMENT IN FRUIT YIELD, QUALITY AND FRUIT DROPING CONTROL IN

KINNOW (CITRUS RETICULATA BLANCO) THROUGH APPLICATION OF GROWTH
REGULATORS, POTASSIUM AND ZINC
M. YASIN ASHRAF1, M. ASHRAF2, M. AKHTER1 AND JAVED AKHTER1
1

Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan

2
Department of Botany, University of Agriculture, Faisalabad, Pakistan

Kinnow fruit (Citrus reticulata) is one of the best commercial fruits of Pakistan. It is cultivated on a large area in the
Punjab province due to its reasonably higher yield, quality, taste and flavor than those of the other citrus fruits.
However, its average yield in Pakistan is far below than that of the other citrus growing countries of the world. Fruit
dropping is one of the promising reasons of low citrus fruit yield in Pakistan, which is thought to be mainly due to
hormonal imbalance in the plants. This imbalance may occur due to nutrient deficiency in soils of orchards, water
shortage and insect pest attack to the citrus trees. Therefore, some experiments were conducted to assess the influence of
growth regulators [2-4 D and salicylic acid (SA)] and nutrients like potassium (K) and zinc (Zn) to improve yield and
quality of citrus fruit and control the fruit drop at four selected sites in the citrus growing tract of Punjab, Pakistan. Foliar
applications of 2-4 D, SA, K and Zn significantly improved the number of fruits per plant, fruit weight, juice percentage,
total soluble solids (TSS), acidity, ascorbic acid, and TSS/acid ratio and reduced the fruit drop. Application of 2-4 D + K
+ Zn and SA + K + Zn showed beneficial effects on all the afore-mentioned parameters.
OPBG 25

TAILORING SOME COARSE AND FINE RICE GENOTYPES FOR LOW-ZINC-INPUT

SUSTAINABLE AGRICULTURE
NIZAMUDDIN DEPAR1*, INAYATULLAH RAJPAR2, MUHAMMAD YOUSUF MEMON1, JAVAID AHMED
SHAH1 AND MUHAMMAD AFZAL ARAIN1
1

Soil Science Division, Nuclear Institute of Agriculture,Tandojam

Department of Soil Science, Sindh Agriculture University Tandojam
*
Corresponding authors e-mail: [email protected]

Rice genotypes differ in their zinc (Zn) deficiency tolerance. Genotypes with high Zn-use-efficiency are considered
part and parcel of the low-Zn-input sustainable agriculture. To explore genotypic differences in response to Zn
deficiency, a hydroponics experiment was conducted with 20 salt-tolerant and salt-sensitive rice genotypes including IR36 as Zn efficient check using completely randomized design. One hundred seeds (sterilized with 1% sodium
hypochlorite for 3 minutes) of each rice genotype were germinated in Petri dishes for 6 days. These seedlings were then
transplanted in an aerobic chelate-buffered nutrient solution. Three levels of Zn activities (2, 10 and 40 pM) were
imposed for 35 days in hydroponics tanks. The pH of nutrient solution was maintained between 5.4 and 5.6 using 3.0
mM 2-(4-morpholino)-ethanesulfonic acid (MES). Phosphorus supplement was checked to avoid toxic levels of P
accumulation at 2 pM (Zn2+) activity. Zinc efficiency varied 26.7 to 71% among the tested genotypes. Seven genotypes
proved to be Zn-efficient, 05 Zn-inefficient, and the left-over 12 were classed as intermediate. The rice genotypes Shua92, Shandar, IR-36 and IR-8 were the most Zn efficient whereas, RG-120, Sarshar and Latifee were observed as most Zn
inefficient. Zn-efficient genotypes extracted more Zn in their shoots than inefficient genotypes, but the correlation
between shoot Zn and shoot dry matter production was poor. All the genotypes accumulated higher concentrations of
iron (Fe), copper (Cu), manganese (Mn), and phosphorus (P) at Zn deficient level as compared to adequate levels.

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OPBG 26

VARIATION AND DISTRIBUTION IN SEED STORAGE STARCH AMYLOSE CONTENT

AND ITS ASSOCIATED 60KD WAXY PROTEIN BAND IN PAKISTAN
RICE GENETIC RESOURCES
SADAR UDDIN SIDDIQUI1,2*, TOSHIHIRO KUMAMARU2 AND HIKARU SATOH2
1

National Agricultural Research Centre, Islamabad, Pakistan; 2 Kyushu University, Fukuoka, Japan.
*
Corresponding authors e-mail: [email protected]

Starch is one of the principal constituent (constituting about 90% of its dry weight) of rice grain determining the
cooking quality. Cooked grain resistance to disintegration is also related to amylose content, with high amylose rice
being the most resistant and waxy rice the least resistant. Due to the importance of variation and mutation in genetical
studies and being material source for improvement in existing varieties, search for diversity (apparent amylose content)
in rice was carried out. A large variation was recorded for the apparent amylose content as determined by colorimetric
method. The amylose content ranged between 5 to 30%. The geographical distribution of amylose content in the four
regions of collection showed that the widest variation was recorded from Khyber Pakhtunkhwa/Northern Areas. In
Pakistan rice cultivars two types of banding pattern was observed for 60kD waxy protein band, which is related to the
synthesis of amylose. The 60kD high type which is Wxa and mainly present in IR36 types was dominant in Pakistan rice
cultivars (89.3%), while, the 60kD low type were only 10.7%. The 60kD high types were distributed in all locations
while the 60kD low types were mainly found in the Khyber Pakhtunkhwa / Northern Areas. This study suggests that
greater diversity exist in Khyber Pakhtunkhwa and NA of Pakistan for starch characteristics and genetic erosion has
occurred in Punjab probably due to introduction of improved varieties.
OPBG 27

PERFORMANCE EVALUATION OF COMMON CLEMENTINE ON

VARIOUS ROOTSTOCKS
SAJJAD HUSSAIN1*, MUHAMMAD AKBAR ANJUM1, FRANCK CURK2,
FRANOIS LURO2 AND GILLES TISON2, 3
1

Department of Horticulture, Univ. College of Agriculture, Bahauddin Zakariya University, Multan60800, Pakistan
2
INRA, UR1103 Gntique et Ecophysiologie de la Qualit des Agrumes, F-20230 San Giuliano, France.
3
AREFLEC Association Rgionale dExprimentation Fruits et Lgumes de la Corse F-20230 San Giuliano, France.
*
Corresponding authors e-mail:[email protected]

Common Clementine (Citrus clementina Hort. Ex Tan.) was grafted on nine rootstocks i.e. Kryder Trifoliate, Towne
Trifoliate, Rich Trifoliate, Beneke Trifoliate, Holansis Trifoliate, AA18 Trifoliate, Gou Tou Sour orange, Da Hong Pao
Mandarin and Carrizo Citrange during 1992. The grafted plants flowered in 1996 and these were evaluated for fruit yield
and quality over a period of 12 years (1996 2007). Fruit yield was inconsistent with great variation when Clementine
scions were grafted on Gou Tou Sour orange and Da Hong Pao Mandarin. The rootstock Carrizo Citrange resulted in
significantly higher and Da Hong Pao Mandarin significantly lower average fruit yields for the last four years of trail.
However, Holansis Trifoliate gave maximum percentage of large sized fruits, followed by Kryder Trifoliate and Towne
Trifoliate. Significantly higher percentage for medium sized fruits was recorded in case of Da Hong Pao Mandarin,
followed by Gou Tou Sour orange. Maximum average fruit weight was recorded for Kryder Trifoliate and Holansis
Trifoliate, followed by Towne Trifoliate, Rich Trifoliate, AA18 Trifoliate, Beneke Trifoliate, Carrizo Citrange and Da
Hong Pao Mandarin. Juice percentage in Clementine fruits was significantly higher for Carrizo Citrange and all the
Trifoliate rootstocks except AA18 Trifoliate which stood at par with Gou Tou Sour orange and Da Hong Pao Mandarin.
Total soluble solids were also significantly higher for all the trifoliate rootstocks followed by the Carrizo Citrange. No
significant differences were found for acidity content of the juice for the rootstocks used. However, TSS: acidity was
significantly higher for Carrizo Citrange, followed by Kryder Trifoliate and Towne Trifoliate. These results indicated that
Carrizo Citrange performed better as compared to other rootstocks while Da Hong Pao Mandarin and Gou Tou Sour
orange were not suitable rootstocks for common Clementine.
OPBG 28

TAGGING GENES FOR VELVET HAIRINESS IN COTTON USING RAPD MARKERS

AND BULKED SEGREGANT ANALYSIS
SAJID-UR-RAHMAN1 AND TANWIR AHMAD MALIK2
1

Agricultural Biotechnology Research Institute, AARI, Faisalabad;

Dept.of Plant Breeding and Genetics, Uni. of Agriculture, Faisalabad.
Corresponding author: [email protected]

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Trichomes also known as hairs are one of the major important morphological parameters of plant resistance to
insects in cotton. The larvae of cotton pink bollworm hatching from eggs laid on the vegetative parts of cotton plants are
disoriented by trichomes and hence, the number of larvae reaching the bolls is reduced. Variations in form, function and
distribution of hairs within a species have been exploited in developing insect resistant cultivars. The DNA marker for
the velvet hairiness trait was identified using Random Amplified Polymorphic DNA (RAPD) technique through bulked
segregant analysis. A cross of velvet hairy and less hairy genotypes was made and segregating F2 generation was
developed for DNA marker studies. Out of the 320 RAPD primers used, 36 showed polymorphism among the parents.
Primer GLG-6975 showed tight linkage with the trait after segregation analysis of the individual F2 plants. This RAPD
marker may be converted into SCAR (Sequence characterized amplified regions) in future for use in molecular breeding
or genetic studies.
OPBG 29

ASSESSMENT OF GENETIC DIVERSITY IN STRIPE RUST RESISTANT NUWYT

LINES USING MICROSATELLITE MARKERS
SANA LIAQAT1, MADIHA SADIQ1, KOUSAIN KOUSAR1, MUHAMMAD FAYYAZ2, FARRAKH
MEHBOOB2, ROBINA KHAN1 AND SUMAIRA FARRAKH1*
1

Department of Bioinformatic and Biotechnology, International Islamic University Islamabad

2
National Agriculture Research Center, Islamabad
*
Corresponding authors e-mail:[email protected]

Stripe rust is one of the most devastating diseases, caused by Puccinia striiformis f. sp. tritici, affecting a huge
amount of wheat crops worldwide. In this study, the genetic diversity of 16 National Uniform Wheat Yield Trial
(NUWYT) candidate lines was evaluated by using 22 screened microsatellite markers. These lines were found resistant
for stripe rust at adult plant stage. Microsatellite markers identified 16 loci and detect a total of 38 alleles, with an
average of 2.375 alleles per locus. The number of alleles ranged from 1 to 5 alleles and the highest number of alleles
were associated with B genome (25), as compared to D (11) and A (2) genomes. The allelic Polymorphism Index
Content (PIC) reflecting the gene diversity of these microsatellite markers ranged from 0.00 to 0.66, with an average of
0.2702. The maximum PIC value of 0.6686 was observed for xgwm 159-5B and 0.6423 for xgwm 413-1B. The gene
diversity ranged from 0.00 to 0.710, with an average of 0.304. The genetic similarity matrix was used to construct a
dendrogram and the cluster analysis was performed by the use if UPGMA algorithm. This divided the entire 16
candidate lines into 3 main clusters on the basis of their similarity. Our results indicate that the genetic diversity among
the 16 candidate NUWYT lines was very narrow.
OPBG 30

SCREENING AND STABILITY ANALYSIS OF SALT TOLERANCE WHEAT

GENOTYPES UNDER FIELDS OF HIGHER SALINITIES
SHAFQAT FAROOQ1, 2*, HAFEEZ SADAQAT3 AND FAROOQE AZAM2
1

Nuclear Institute for Agriculture and Biology (NIAB), P. O. Box 128, Jhang Road, Faisalabad
Pakistan Institute of Engineering and Applied Sciences (PIEAS), P. O. Box NILORE, Islamabad
3
Department of Plant Breeding and Genetics, University of Agriculture Faisalabad
*
Corresponding authors email: [email protected]
2

Potential salt tolerant genotypes that could survive under saline fields of Electrical Conductivity (EC) ranging
between 15-20 dS m-1 were selected for screening under salinity levels ranging between ECe20-25dS m-1. The objectives
were i) to test the extent of salt tolerance in the test material and ii) identification of and stable wheat genotype.
Experiment of was conducted in the saline fields of Pacca Ana near Faisalabad in 2004 in split plot design with three
replications: salinity treatments as main plot and wheat genotypes as subplot. The harvesting was made from the one
meter square plot and grain yield (Gram plot-1) was calculated. Of the ten genotypes, 3 along with both tolerant and
sensitive varieties planted as check could not germinated. The remaining 5 genotypes exhibited grain yield in the range
of 14.4-1.6 g m-2. These five genotypes along with sensitive check were studied for stability parameters. Analysis of
variance for genotypes, environment and their interaction based on means over replicate indicated highly significant
(p<0.001) differences. Three lines deviated non-significantly, one significantly (p<0.05) while the sensitive check and
genotype WL-1073 deviated highly significantly (p<0.001) from zero. Overall mean values for grain yield also differed
significantly. Two lines exhibited grain yield more than the average yield of all the six genotypes. Linear regression
coefficient (bi) and deviation from regression (S2d): the two stability parameters also indicated significant differences

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(p<0.01 and 0.001) in individual genotypic response towards salinity as their values ranged between 0.37 (L-9) and 1.84
(L-22). None of the genotypes exhibited bi-value equal to 1.0 although, value of Chakwal-97 was nearly equal to 1.0
while WL-1073 exhibited the highest bi-value (1.84) followed by WL-1076 (1.64) and Inqlab-97: sensitive check (0.75).
Significant variations were also observed in deviation from regression. None of the genotypes exhibited S2d value equal
to 0. This paper will describe details and significance of these observations.
OPBG 31

GENETIC DIVERSITY IN BASMATI AND NON-BASMATI RICE VARIETIES

BASED ON MICROSATELLITE MARKERS
SHAHID MASOOD SHAH, GHULAM SHABIR, MUHAMMAD SABAR, KASHIF ASLAM, JAVED IQBAL
WATTOO, SHAHZAD AAMIR NAVEED, NOURIN ASHIQ, RAHEELA WAHEED AND MUHAMMAD ARIF

National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan Institute of Engineering and Applied
Sciences (PIEAS), Nilore, Islamabad, Pakistan
Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. The purpose of
this study was to evaluate the genetic diversity within a diverse collection of rice (Oryza sativa L.) accessions, and to
determine differences in the patterns of diversity within the aromatic and non-aromatic rice varieties. Forty rice
accessions were evaluated by means of 24 microsatellite markers distributed over the whole rice genome. A total of 66
alleles were detected at 24 SSR loci, and the number of alleles per marker ranged from 2 to 4, with an average of 2.75.
Polymorphism information content (PIC) value ranged from 0.0476 (RM315) to as high as 0.5993 (RM252), with an
average of 0.3785 per marker. The average genic diversity over all SSR loci for the 40 genotypes was 0.4477, ranging
from 0.0488 to 0.6638. Major allele frequency ranges from 0.4250 to 0.9750, with an average of 0.6472. The
dendrogram based on the cluster analysis by microsatellite polymorphism, grouped 40 rice cultivars into three groups
effectively differentiating basmati cultivars from non-aromatic cultivars. These results could be useful for monitoring
purity, genotype identification and for plant variety protection.
OPBG 32

INTEGRATION OF NOVEL CHLOROPHYLL GENES FROM BLACK PINE

INTO THE CHLOROPLAST GENOME OF TOBACCO
SHAHID NAZIR AND MUHAMMAD SARWAR KHAN*
National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road Faisalabad, Pakistan
Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad Pakistan
*Corresponding authors e-mail: [email protected]
Gymnosperms owing the presence of dark-operative protochlorophyllide oxidoredcuatse pathway are able to reduce
protochlorophyllide to chlorophyllide leading to the formation of chlorophyll in dark, whereas angiosperms are unable to
do this and are less photosynthetically efficient. Plastid encoded genes chlL and chlN are reported to be involved in the
functioning of the enzyme Dark-operative Protochlorophyllide Oxidoreductase (DPOR). The genes were isolated from
black pine and cloned into the species-specific chloroplast targeting vector; harboring FLARE-S, a fluorescent selection
marker. The final transformation vector was used to introduce genes into the tobacco chloroplast genome using biolistic
approach. Here, we report stable integration of both genes along with translationally fused marker genes (gfp and aadA)
into the inverted repeat region of plastome which was confirmed by Polymerase Chain Reaction and Southern blot
analysis. Morphological and physiological analyses of the transgenic plants compared with non-transformed wild type
tobacco plants revealed that the activation of dark-operative pathway requires additional factors/genes to chlL and chlN
genes to regulate the plant cell machinery.
OPBG 33

GENETIC DIVERGENCE IN TARAMIRA (ERUCA SATIVA L.) GERMPLASM BASED

ON QUANTITATIVE AND QUALITATIVE CHARACTERS
SHEHLA SHINWARI1, ABDUL SAMAD MUMTAZ1*, M. ASHIQ RABBANI2, FAZAL AKBAR3
AND ZABTA KHAN SHINWARI3
2

1
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Institute of Agri-Biotechnology & Genetic Resources, NARC, Islamabad, Pakistan
3
Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan
*
Corresponding authors Email: [email protected]

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The breeding potential of the Eruca sativa (Taramira) genotypes held in IABGR gene-bank has not been exploited
to date. A total of 100 Eruca sativa genotypes collected from various eco-geographical regions of Pakistan were assessed
to estimate the phenotypic diversity for various quantitative and qualitative characters. A significant level of
morphological diversity was recorded for a number of traits. The correlation coefficient analysis suggested that some
traits had significant positive correlation with seed yield. Multivariate analysis was executed in order to set up similarity
and dissimilarity patterns. Principal component (PC) analysis explained a high level of variation for most of the
qualitative and quantitative agronomic characters. Cluster analysis suggested that genotypes were mainly grouped due to
their morphological dissimilarities. Best Taramira accessions have been chosen on the basis of their most excellent
agronomic performance from 100 genotypes. Our findings have an important application for Eruca sativa germplasm
evaluation, improvement, classification and preservation in Pakistan.
OPBG 34

THE EFFECT OF NITROGEN RATE AND PLANT DENSITY ON MORPHOLOGICAL

TRAITS AND ESSENTIAL OIL YIELD OF CORIANDER

SEYYED GHOLAMREZA MOOSAVI*, MOHAMAD JAVAD SEGHATOLESLAMI, AMIR EBRAHIMI

AND ZEINOLABEDIN JOUYBAN
1

Islamic Azad University, Birjand Branch, Birjand, Iran

Corresponding authors e-mail :[email protected]

In order to study the effect of nitrogen rate and plant density on morphological traits and essential oil and fruit yield
of coriander, a split-plot experiment was carried out in research field of Islamic Azad University, Birjand Branch, Birjan,
Iran in 2010 based on a randomized complete block design with three replications. The main plots were nitrogen rates at
four levels (0, 40, 80 and 120 kg N/ha) and the sub-plots were plant densities at three levels (30, 40 and 50 plants/m2).
The results showed that nitrogen rate had significant effect on fruit yield, essential oil percent and yield traits and
interaction between nitrogen rate and plant density t only affected fruit yield but change in plant density significantly
affected all traits except essential oil percent. Means comparison showed that as N fertilization rate was increased from 0
to 80 kg N.ha-1, plant height and fruit yield were increased by 19.8 and 74.1 %, respectively. Also, essential oil percent
increased from 0.153 to 0.33% and essential oil yield was grater 2.68 times. Moreover, means comparison showed that
the increase in plant densityfrom 30 to 50 plants/m2 increased plant height, first fruit distance from ground, fruit and
essential oil yield by 14.3, 27.6, 31.3 and 36.8%, respectively while stem diameter and branch number per main stem
were decreased by 22.2 and 13.9%, respectively. Given the results of the study, the treatment of 80 kg N/ha application
with the density of 50 plants/m2 recommended for the cultivation of coriander in Birjand, Iran.
OPBG 35

ANTIOXIDANT ACTIVITXY AND PROTECTING ABILITY OF DIFFERENT

CULTIVARS OF SUGARCANE AGAINST DNA DAMAGE
SYED MUBASHAR SABIR1*, SYED RIZWAN ABBASS2, SYED ASAD HUSSAIN SHAH2
AND SYED DILNAWAZ AHMAD2

1
Department of Eastern Medicine and Surgery, University of Poonch Rawalakot Azad Kashmir Pakistan
Department of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot, Azad Kashmir Pakistan
*
Corresponding authors email: [email protected]

High intake of natural antioxidants has been associated with lower incidence of chronic diseases such as cancer and
heart diseases. Thus, there is need to find out the diet rich in antioxidants and phenolics. Saccharum officinarum was
carried out to evaluate and explore new potential sources for natural antioxidants. DNA damage caused by exposure to
reactive oxygen species is one of the primary causes of DNA decay in most organisms. Antioxidant activity was
determined by 2,2-Diphenyl-1-picrylhydrazyl method. The phenolic content was determined by Folin-Ciocalteu,s
reagent. Whereas, site specific DNA damage was induced by using Fe+H2O2 and the gel pattern was studied using
agarose gel electrophoresis. The aqueous extracts of leaves of thirteen varieties of sugarcane were studied for their
antioxidant activity and protective effect on DNA damage. These varieties showed good antioxidant properties (IC50
values ranged from 18.751.2 to 27.670.45 g/ml) and possess strong capability to protect DNA damage induced by
hydroxyl radical generated in Fenton reaction. The high antioxidant activity and DNA protecting ability of sugarcane
may be due to their phenolic content.

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OPBG 36

CLIMATE CHANGE IMPACT ON SUNFLOWER PRODUCTIVITY UNDER AGROENVIRONMENTAL CONDITIONS OF PAKISTAN: SIMULATION & FIELD STUDY
WAJID NASIM1, 2*, MUHAMMAD FARROQ HUSSAIN MUNIS1, HASSAN JAVED CHAUDHARY1
AND MUHAMMAD SHAHID1
1

Department of Plant Sciences, Quaid-i-Azam University, Islamabad (45320), Pakistan

Department of Environmental Sciences, COMSATS Institute of Information Technology (CIIT-Vehari) 61100, Pakistan.
*
Corresponding authors Email: [email protected]

Sunflower is the worlds third most important oil-producing crop, accounting for about 13% of the total world edible oil
production. Local availability of edible oilseeds in Pakistan hardly meets the demand and this is the scenario since decades. It is
much susceptible to climatic variables and hence climate could have significant effect on its production. The present study of
simulation evaluates the impact of climate change on sunflower crop in contrasting agro-environments of the Punjab-Pakistan.
The validated model (OILCROP-SUN model) has reasonably predicted phenology, crop growth and yield of sunflower crop.
The crop was found to be sensitive to changes in carbon dioxide (CO2) and temperature. Future climate change scenario
analysis showed that sunflower yields are likely to reduce in both irrigated and rainfed conditions. The increase in CO2
concentration from 360 ppm to 550 ppm has no affect on crop duration at different experimental locations. On the other hand,
total dry matter (TDM) will increase with increased CO2 concentration. Difference in the average values of crop duration in
sunflower hybrids were 2.22%, 3.88% and 4.37% at Multan, Faisalabad and Gujranwala experimental locations, respectively.
The achene yield was also significantly affected by the increase in CO2 concentration from 360 ppm to 550 ppm at all the
experimental locations. These effects gave maximum with differences of 4.23% at Gujranwala, followed by Faisalabad with
the difference of 3.87% and minimum difference 2.14% was observed at Multan experimental locations, respectively with 550
ppm CO2. Results of present study showed that, increasing temperature will shorten crop duration from planting to
physiological maturity (with difference of almost 14 days in 2020's and 21 days in 2050's, respectively), thus retards the growth
and development and ultimately decreased the yield than current situation at all the experimental locations. Future studies for
possible effects of climate change on field crop production should therefore includes, more number of weather stations to better
reflect the heterogeneity in different cropping areas. Standardization of field crop production technology through crop growth
models should be initiated. Development of site specific mitigation strategies to enhance agricultural productivity under
changing climate scenarios.
OPBG 37

AGRONOMIC EVALUATION OF COTTON (GOSSIPIUM HIRSURUM L.) ADVANCED

STRAINS FOR FIBER QUALITY AND YIELD PARAMETERS UNDER
TANDO JAM ENVIRONMENTAL CONDITIONS
Z.A. DEHO, S. LAGHARI, S. ABRO, M.A. ARAIN, S.D. KHANZADA AND FAKHRUDDIN
Plant Breeding and Genetics Division, Nuclear Institute of Agriculture (NIA) Tando Jam
Corresponding author: [email protected]
Forty three advanced strains of cotton (Gossypium hirsutum L.) were evaluated along with three commercial check
varieties Sadori NIA-ufaq and CRIS-134 at Nuclear Institute of Agriculture (NIA), Tando jam during 2011-2012 for their fiber
quality and yield parameters. The trial was conducted in RCBD with three replicates. Five plants from each entry of random
selected for taking fiber quality and yield parameters. The data were recorded on sympodial branches plant-1, boll weight plant1
, seed index (g), staple length (mm), ginning out turn %, micronaire g inch -1 and seed cotton yield kg ha-1. Five strains were
higher (26-28) in sympodial branches plant -1 than check varieties (24-18), seven strains were boll weight ranging from (3.06g 3.50g) than check varieties (2.27 g - 2.57g). Eight strains gained higher seed index ranging from (7.06g to 7.76g) than check
varieties (6.50g - 6.86g),eleven strains possessed more staple length (28.03mm - 29.83mm) than check varieties (27.14mm 28.00mm). Five strains were higher in ginning out turn percent (36.47% - 40.68%) than check varieties (35.42% - 36.37%).
Two strains took fine fiber (3.36-3.38) and rest of strains and checks are medium micronaire values (4.00-4.40). Similarly
seven advanced strains had significantly more seed cotton yield kg ha -1 ranging from (4240-4867 kg ha -1) than check varieties
(2959-4100 kg ha -1).

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POSTER ABSTRACTS
PPBG 1

PHENOLOGY AND ACCUMULATIVE HEAT UNIT OF VARIOUS WHEAT CULTIVARS

UNDER LATE SOWING HIGH TEMPERATURE
ABDUL SATTAR1*, MUMTAZ A. CHEEMA1, M.A. WAHID1, M.F. SALEEM1, H. MUNIR2,
FAHD RASUL1 AND N. SARWAR1
1

Department of Agronomy, University of Agriculture, Faisalabad-38040, Pakistan

Department of Crop Physiology, University of Agriculture, Faisalabad-38040, Pakistan
*
Corresponding authors e-mail: [email protected]

Delay in wheat sowing in rice-wheat cropping system is perhaps the one of the major factors responsible for low
crop yield. This reduction in yield is due to the sub-optimal temperature during the different phenological stages of
wheat crop. Therefore, phenological performance of five newly developed wheat varieties (viz. Lasani-2008,
Faisalabad-2008, Shafaq-2006, Sahar-2006 and Inqlab-91) were evaluated under two growing environments; one is
normal growing environment (sowing at November 10) and the other is heat stressed environment (sowing at December
10). Results indicate that normal sowing took more number of days to attain different phenological stages, higher
accumulative heat unit and heat use efficiency as compared to the late sowing. In case of late sowing, the varieties
phased a significant level of high temperature stress that also significantly affected the required days to attain crown
roots, tillering, booting, heading, anthesis, grain filling and maturity of all varieties including the yield as compared to
normal sowing. Under late sowing high temperature, Faisalabad-2008 took maximum days and higher accumulative heat
unit to attain all phenological stages. To conclude variety Faisalabad-2008 ontogenically being more plastic performed
well under late sown high temperature conditions. Nonetheless variety Iqlab-91 was at the top when planted timely.
PPBG 2

SOME PHYSIOLOGICAL AND GENETICAL DETERMINENTS OF SALT TOLERANCE

IN SORGHUM (SORGHUM BICOLOR L.): BIOMASS PRODUCTION AND
SALINITY MEDIATED NITROGEN METABOLISM
ABIDA KAUSAR1, M. YASIN ASHRAF2*, MUBASHIR NIAZ1, M, ASHRAF3 AND QAISER ABBAS1
1

Department of Botany, GC University, Faisalabad, Pakistan;

Nuclear Institute of Agriculture and Biology, Faisalabad, Pakistan;
3
Department of Botany, University of Agriculture, Pakistan
Corresponding authors e-mail:[email protected]

Salinity stress limits the crop production due to which shortage in food, feed and industrial raw materials are being
observed all over the world. The plant productivity can be increased by introducing salt tolerant crop cultivars.
Therefore, a study to identify the physiological and biochemical determinants for salt tolerant in sorghum was conducted
under greenhouse conditions. Physiological and biochemical attributes i.e., biomasss, proteins, total nitrogen, total free
amino acids, nitrate reductase activity (NRA) and nitrous oxide were estimated in two salt tolerant and two salt sensitive
genotypes/cultivars of sorghum. Salinity stress reduced biomass mass of shoots (22.3%) and roots (24%), total proteins
(34%), NRA (13%), nitrous oxide (54%), total nitrogen of shoots (52%) and roots (45.4%) while increase in total free
amino acids (37%) were recorded for all the sorghum genotypes/cultivars. On the basis of results obtained using the
above physiological and biochemical traits, sorghum genotypes/cultivars JS-2002 and Sandalbar were categorized as
tolerant, Noor medium sensitive and FJ-115 as sensitive one. The results also indicated that the physiological and
biochemical attributes can be used to identify the salt tolerant and sensitive sorghum genotypes/cultivars leading.
PPBG 3

MODELING GROWTH, DEVELOPMENT AND SEED COTTON YIELD OF PROMOSING

COTTON CULTIVARS AT VARYING NITROGEN INCREMENTS WITH
DIFFERENT PLANTING DATES UNDER DSSAT
AFTAB WAJID1, ASHFAQ AHMAD1*, GERRIT HOOGENBOOM2, TASNEEM KHALIQ1 AND M. USMAN1
Agro-Climatology Lab. Dept of Agronomy University of Agriculture, Faisalabad, Pakistan
2
Washington State University, USA
*
Corresponding Author: [email protected]

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

18

CSM-CROPGRO-Cotton Model under DSSAT V 4.0.2.0 was used for dynamic simulation of development, growth
and seed cotton yield of four cotton cultivars (CIM-496, CIM-506, NIAB-111 and SLH-284) at varying N increments
(50, 100, 150 and 200 kg ha-1 sown at different timings (20 May and 10 June) at three locations (Faisalabad, Multan and
Sahiwal). Model performance was satisfactory for crop phenology at all locations with a % error of 1.49 and RMSE
0.68. According to model simulations May sown crop maturity ranged between 172 to 176 days as against June sown
which took between 147 to 153 days which were in close proximity with the observed values at three locations. As
regards canopy development the simulated and observed values were much closer to each other with low root mean
square error ranging from 0.79 to 1.26. Leaf area index prediction was 4.17 compared to observe 4.16 values for May
sown and 3.22 to 3.17 in June sown crop. Coefficient of regression for the pooled data was 0.87 but values were higher
at different locations. Model overestimated total dry matter at all locations with low RMSE of 289.52 kg ha-1 giving
strong relationship of 95 % between simulated and observed data. CROPGRO-Cotton Model over predicted by 8 % of
simulated seed cotton yield in early sown than late sown. Root mean square error for low Nitrogen (50 kg ha-1
application crop was 42-198 kg ha-1 than high dose of N (200 kg ha-1) that ranged between 95-195 kg ha-1. Coefficient of
regression for different locations ranging from 0.93 to 0.99 and for pooled data 0.994 %. Overall Model performance
under DSSAT was good. There is a dire need to assess impact of climate variation on seed cotton yield under various
climatic regions to ensure fiber quantity in future.
PPBG 4

PHYSIOLOGICAL EXPRESSIONS AND DRY MATTER PRODUCTION OF MAIZE (ZEA

MAYS L.) IN RESPONSE TO TILLAGE AND NITROGEN APPLICATION
ALLAH WASAYA1*, FAYYAZ-UL-HASSAN1, MUHAMMAD TAHIR 2, MUHAMMAD ANSAR1
AND ABDUL MANAF1
1

Department of Agronomy, PMAS- Arid Agriculture University Rawalpindi, Pakistan

2
Department of Agronomy, University of Agriculture Faisalabad, Pakistan
*
Corresponding authors email: [email protected]

Subsoil compaction and injudicious use of nitrogenous fertilizers are the key factor for lowering the maize yield which
can be improved by ameliorating the subsoil compaction and proper application of nitrogen. Physiological expressions and
dry matter production of maize (Zea mays L.) in response to tillage and nitrogen application was studied through two years
field experiments conducted at University of Agriculture Faisalabad, Pakistan during 2008 and 2009. The experiments
comprised of three tillage systems (conventional tillage, tillage with mouldboard plough + 2-cultivations and tillage with
chisel plough + 2-cultivations) and three nitrogen rates (100, 150 and 200 kg ha-1) laid out in randomized complete block
design (RCBD) with split plot arrangement replicated thrice. Tillage systems and nitrogen levels significantly affected
physiological attributes and dry matter production in maize. Significantly larger leaf area index, leaf area duration, crop
growth rate and dry matter obtained from chisel tilled plots compared with other tillage systems. Increasing nitrogen
application rate resulted in increased maize physiological attributes and dry matter production. Significantly larger leaf area
index, leaf area duration, crop growth rate, dry matter production and harvest index recorded with 200 kg ha-1 nitrogen
application. Therefore, it may be concluded that maize hybrids should be grown with 200 kg ha-1 nitrogen application by
preparing the field with chisel plough followed by cultivator to obtain higher dry matter yield.
PPBG 5

PRELIMINARY STUDIES OF ASSOCIATION MAPPING IN COTTON

AMMAD ABBAS1, YUSUF ZAFAR1, MUHAMMAD ATIF1, ANDREW H PATERSON2
AND MEHBOOB-UR-RAHMAN1*
1

National Institute for Biotechnology & Genetic Engineering (NIBGE), PO Box 577 Jhang Road Faisalabad Pakistan
2
Plant Genome Mapping Laboratory, University of Georgia, Athens, 30602, GA, USA
*
Corresponding authors email: [email protected]

To the extent of our knowledge conventional DNA marker studies and finding their utilities in marker-assisted
breeding are handicapped in cotton due to low genetic diversity. Cotton leaf curl disease (CLCuD), a disease of viral
origin, has substantially depresses cotton production in Pakistan, thus a major handicap in achieving sustainability.
Disease was also reported in neighboring countries like China and India. The present study was designed to find out the
DNA markerspredominately simple sequence repeats (SSRs) associated with the genes conferring tolerance and or
resistance to CLCuD followed by testing their efficacy in marker assisted breeding in cotton. Initially, a total of 10
cotton genotypes (five highly tolerant and five high susceptible) of diverse origin were selected for the study including
nine Gossypium hirsutum accessions and one Gossypium arboretum accession (immune to the disease). We surveyed
322 SSRs derived from bacterial artificial chromosome (BAC) ends of Gossypium raimondii genome sequences. Out of
these, 65 primer pairs were polymorphic. The polymorphism percentage was 20.18 and the extent of genetic similarity
was in the range of 81.7% to 98.7%. The similarity matrix was used for studying their phylogenetic relationship using

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

19

unweighted pair group method of arithmetic means (UPGMA). Dendrogram showed grouping of genotypes in two
distinct clusters comprising of tolerant and susceptible genotypes except G. arboreum confirming its distinct origin. The
G.arboreum is a diploid species and is one of the progenitors of cultivated tetraploid species. Out of the polymorphic
markers, two SSR markers PR-91 and CM-43 that were amplified in tolerant genotypes which were subsequently
surveyed on 185 cotton genotypes. The markers were present in 23 highly tolerant cotton genotypes building a strong
association between resistance trait and marker. These preliminary results set a stage for doing in depth marker-trait
association studies which will be instrumental for initiating marker assisted selection.
PPBG 6

ASSESSMENT OF SALT TOLERANCE AMONG DIFFERENT SUNFLOWER ACCESSIONS

AMMARA RAUF1, FAROOQ A. KHAN1, MUHAMMAD ASLAM1 AND MUHAMMAD NADEEM ANWAR2
1

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad.

2
The European Sixth Form College, Malaga, Spain.
*
Corresponding authors email: [email protected]

Twenty sunflower (Helianthusannuus L.) accessions were evaluated against three different salinity levels.
Triplicated completely randomized design was followed. Salinity was developed with NaCl to achieve the final salinity
levels of 3dSm-1, 6 dSm-1, and 9 dSm-1, whereas control contained tap water. Data of 60 days old ten seedlings from each
entry was recorded and analyzed. Accessions G-36, G-61, A-23, A-6, and A-185 performed better in both controlled and
saline conditions. These accessions showed better biomass production and high shoot and root growth by least
concentration of Na+ and higher concentration of K+ and Cl- in leaf sap resulting in better K+:Na+.
Key words: Sunflower, salinity, genetic variability.
PPBG 7

EVALUATION OF GENETIC DIVERSITY IN BREAD WHEAT

(TRITICUM AESTIVUM L.) BY SDS-PAGE ANALYSIS
AMJAD UR RAHMAN* AND ABDUL SAMAD MUMTAZ
Department of Plant Sciences, Quaid-i-Azam University, Islamabad-Pakistan
*
Corresponding authors email: [email protected]
Genetic diversity of wheat varieties are of great interest in reducing genetic vulnerability which lead to stable control
of production. Wheat seed-storage proteins from 42 wheat genotypes were analyzed by sodium dodecyl sulphate
polyacrylamide gel (SDS-PAGE). Electrophorogram for each variety were scored and Jaccards similarity index (JSI)
was calculated. Genetic diversity of wheat was evaluated via unweighted pair group method with arithmetic averages
(UPGMA) cluster analysis by constructing dendrogram for high and low molecular weight (HMW, LMW) gluten
subunit bands. The greatest similarity index (97.4%) was observed between 2SRRSN-6038 and Zarghoon-79, while the
lowest similarity index (31.6%) was observed between 2SRRSN-6013 and Abadgar-93. It is concluded that seed storage
protein profiles could be useful markers in the studies of genetic diversity and classification of genotypes, which can be
used to improve the efficiency of wheat breeding programs in cultivar development especially in a developing country
like Pakistan.
PPBG 8

BIOASSAYS GUIDED FRACTIONATION OF CHENOPODIUM ALBUM L. FOR

EVALUATION OF ITS ANTIFUNGAL ACTIVITY TO CONTROL ONION BASAL ROT
PATHOGEN FUSARIUM OXYSPORUM F. SP. CEPAE
ARSHAD JAVAID* AND SAIMA RAUF
Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
Corresponding authors e-mail: [email protected]
Present study was carried out to investigate the antifungal activity of different parts of Chenopodium album for the
management of Fusarium basal plate rot disease of onion (Allium cepa) caused by Fusarium oxysporum f. sp. cepae. In
laboratory bioassays, the effect of methanolic leaf, stem, root and inflorescence extracts of 0.5, 1.0,3.0% (w/v) of C.

20

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

album was investigated against growth of the target fungal species. All the extracts showed variable antifungal activity.
Inflorescence extract was found to be the most effective where different concentrations of the extract reduced the fungal
biomass by 2480%. Methanolic inflorescence extract was successively extracted with n-hexane, chloroform, ethyl
acetate and n-butanol in increasing order of polarity. Ethyl acetate fraction exhibited the highest antifungal activity
resulting in 68-100% reduction in fungal biomass. Three fractions viz. A, B and C were isolated from ethyl acetate
fraction of inflorescence extract through thin layer chromatography (TLC). TLC fraction A exhibited the highest
antifungal activity with minimum inhibitory concentration (MIC) of 250 g mL-1. The present study concludes that
antifungal constituents of ethyl acetate fraction of methanolic inflorescence extract of C. album can be used as natural
fungicides for the control of basal plate rot pathogen of onion.
PPBG 9

EXPLORING THE GENETIC POTENTIAL OF PLANTAGO OVATA GERMPLASM

ASIF JAVAID*, ABDUL GHAFOOR AND M. SHAHID MASOOD
Plant Genetic Resources Program, Institute of Agri. Biotechnology & Genetic Resources, National Agricultural
Research Center, Park Road, Islamabad, Pakistan
*
Corresponding authors email: [email protected]
Plantago ovata, commonly known as Ispaghol in Pakistan, is an important medicinal plant. Research about its
medicinal properties is expected to increase its economic value. However, genetic potential of available Plantago ovata
germplasm is not fully explored for utilization in crop improvement. The present experiment was conducted to study the
genetic potential of 47 Plantago ovata accessions from India(4), Mexico(1), USA(27) and different parts of Pakistan(15)
being stored at Gene Bank of Plant Genetic Resources Program, NARC, Islamabad. The experiment was planted at
Cholistan Institute of Desert Studies, Bahawalpur and data were recorded for agronomic traits including plant height,
tillers per plant, inflorescence length, 1000-grain weight and grain yield. Significant genetic variation was found for
tillers per plant and grain yield while low variance was observed in case of plant height, inflorescence length and 1000grain weight. Nine accessions were observed with grain yield of 60-80g per row with row length of 2 meters while three
accessions had grain yield above 80g per row. These accessions are selected for future use in crop improvement. Cluster
analysis using UPGMA for five agronomic traits divided the accessions into four major clusters that helped in describing
the genetic diversity of germplasm.
PPBG 10

TILLAGE AND CROP SEQUENCE EFFECT ON SOIL ORGANIC CARBON FRACTIONS

AND AGGREGATE STABILITY IN DRYLAND POTHWAR, PAKISTAN
ASMA HASSAN, SHAHZADA SOHAIL IJAZ, SAFDAR ALI, MUHAMMAD ANSAR,
KHALID SAIFULLAH KHAN AND QAISER HUSSAIN
Department of Soil Science & SWC PMAS-Arid Agriculture University, Rawalpindi
Corresponding authors email: [email protected]
Soil organic carbon (SOC) is emerging as a key indicator for assessing sustainability of soil and crop management
systems. Tillage systems and crop sequences greatly alter the SOC fraction but the effects are site specific. A field
experiment was conducted in a warm subhumid subtropical dryland Pothwar of Pakistan to evaluate the effect of
different tillage systems and crop sequences on soil organic carbon fractions. The treatments were arranged in a split plot
design having tillage treatments (conventional tillage, CT; minimum tillage, MT and moldboard plow, MP) in main plots
while crop sequences (FallowWheat, FW; MungbeanWheat, MW; sorghumwheat, SW; green manurewheat, GW
and mungbeanchickpea, MC) in subplots. The highest C mineralization was observed under MT tillage in combinations
with SW, GW and MW rotations (447.67, 441.73 and 406.40 g g-1 soil day-1 respectively). The highest particulate
organic carbon (POC) was observed in the combination of MP tillage with FW crop sequence (12.17 Mg ha-1), while
least POC was under CC tillage with MW crop sequence (2.82 Mg ha-1) and MT tillage with GW crop sequence (2.91
Mg ha-1). Total organic carbon varied non-significantly between 11.45 to 12 89 Mg ha-1 for tillage systems and between
8.89 to 14.41 Mg ha-1 for crop sequences. The highest proportion of stable aggregate was observed under combinations
of MT tillage with SW and MC sequence (31.25 and 27.22%, respectively), while the least aggregate stability was
observed under MP tillage with MC sequence (5.00 Mg ha-1). It is concluded that minimum tillage and elimination of
fallow enhances active SOC fraction and soil aggregation under subtropical dryalnd conditions.

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

21

PPBG 11

INCIDENCE OF BOTRYODIPLODIA THEOBROMAE ON GUAVA ORCHARDS IN

DISTRICT SHEIKHUPURA ITS CHEMICAL MANAGEMENT
ASMA SAFDAR1*, SAJID ALEEM KHAN2 AND M. A. SAFDAR3
1

Rice Research Institute, Kala Shah Kaku

Department of Plant Pathology, University of Agriculture, Faisalabad
3
Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad
*
Corresponding authors email: [email protected]
2

A survey was carried out in District Sheikhupura for the assessment of guava decline. Maximum disease prevalence
(100%) and disease incidence (36%) was recorded in Tehsil Sharaqpur Samples of plant roots, shoots and soils were
randomly collected for the isolation and identification of pathogens. Colonization percentage of the Botryodiplodia
theobromae in 1987 tissues of 326 samples was counted to be (48.84%) maximum, followed by Fusarium oxysporum
f.sp. psidii(44.10%), Phytophthora parasitica (38.10%), Fusarium solani (35.10%) Helminthosporium spp. (15.20%)
and Curvularia spp., (11.20%). Aspergillus flavus and Aspergillus nigerwere also isolated from the samples but mostly
from twigs. Rhizopus spp. was the least frequent fungi. Isolated fungi were multiplied and purified on PDA. Most
dominating isolated fungus (Botryodiplodia theobromae) was evaluated for pathogenicity. Management of the B.
theobromae was done under in vitro and in vivo conditions. In vitro efficacy of seven fungicides viz. Carbendazim,
Thiophanate-methyl, Alliette, Acrobat MZ 75/667WP, Dithan M-45 80% WP, Mancozeb 80% WP and Metalaxyl plus
Mancozeb 72% WP was evaluated against Botryodiplodia theobromae by Inhibition zone technique at different doses
viz. recommended (R), 0.75R, 0.50 and 0.25R. All the employed doses of the test fungicides significantly reduced the
biomass of the test fungal specie but recommended dose rate reduced more significantly. In field experiment,
Carbendazim was found to be more effective than other fungicides checked in reducing the fungal infection in guava
trees, suppressing the dieback and wilting resulting in significant enhancement in vegetative growth of plants.
PPBG 12

ANTIOXIDANT ACTIVITY AND LIPID PEROXIDATION OF SELECTED WHEAT

CULTIVARS UNDER SALT STRESS
AURANGZEB RAO1*, SYED DILNAWAZ AHMAD1, SYED MUBASHAR SABIR2, SHAHID AWAN1, ASAD
HUSSAIN SHAH1, SYED RIZWAN ABBAS1, SAIMA SHAFIQUE, SARDAR ALI AND FAREED KHAN1
1

Department of Plant Breeding and Molecular Genetics, University of the Poonch Rawalakot Azad Kashmir Pakistan
2
Department of Eastern Medicine and Surgery, University of the Poonch Rawalakot Azad Kashmir Pakistan
Corresponding authors email: [email protected]

Antioxidants and dietary fibers are compact sources that are recommended for healthy diets in whole grain foods.
The antioxidant activity, total phenolic content and lipid peroxidation was estimated among fifteen cultivars of wheat
which are commonly used in Pakistan and the effect of salt stress on the antioxidant activity and phenolic content was
also evaluated. The two parameters had a strong correlation (r = 0.98) for diverse array of wheat. The results revealed
that different concentrations of wheat (25-300 g/ml) showed antioxidant activity under salt stress (i.e. electrical
conductivity, 2EC, 4 EC, 8 EC and 16 EC). The inhibitory concentration (IC50 values) of different cultivars of wheat
ranged from 22.9 to 27.01 g/ml. On the basis of comparison of scavenging percentage of non-stress and stressed wheat
groups, we have concluded that LU26-CTR, PAS-90, BARS, NARC, WAFAQ, LISANI, SEHAR, MEHRAJ and
SHAFAQ are salt tolerant varieties. The change of phenolic content under salt stress suggests that wheat uses antioxidant
properties of phenolics as a mechanism of salt stress. Whereas, the results of lipid peroxidation has indicated that LU26CTR, PAS-90, BARS, NARC, FSD-08, PIRSBAK-09, SEHAR and SH-03 are salt resistant varieties as they showed a
less percentage increase in lipid peroxidation (malondialdehyde content) compared to control at maximum dose of salt.
PPBG 13

NUTRITIONAL PROFILE, MYCOFLORA ASSESSMESNT AND AFALTOXIN

CONTAMINATION IN CHICKPEA (CICER ARIETINUM L.)
AYSESHA TANIA1, ABIDA AKRAM1, NAFEESA QUDSIA HANIF2*, MUHAMMAD ARSHAD1
AND ABDUL RAUF1
1

Department of Botany, PMAS - Arid Agriculture University Rawalpindi, Romer Labs, Pakistan Rawalpindi,
2
Romer Labs, Pakistan-Rawalpindi
*Corresponding author: [email protected]

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

22

Chickpea (Cicer arietinum L.), a very common and cultivated crop is belonging to family Fabaceae. During present
study, fifty samples (n=50) collected from three arid districts of Punjab put forth for assessment of mycoflora, aflatoxins
contamination and nutritional profile. The results revealed sixteen fungal isolates belonging to 6 fungal generas were
observed in chickpea samples. Observations revealed that A. niger, A. flavus, mucor and Alternaria alternata, were
predominant fungi. A total of 333 fungal isolates were identified. Co-occurrence of fungal isolates showed maximum
number of fungal isolates were twelve out of sixteen (n=16), whereas, minimum number of fungal isolates were only two.
The comparative study of mycoflora of three districts of Punjab showed that highest incidence of fungal flora was found in
samples collected from district Rawalpindi (12 isolates) followed by Khushab district (117 isolates) and district Chakwal
(isolates 96). Among chickpea samples, only two samples were found positive for aflatoxin B1 with value 19ppb and 18 ppb
of samples Khushab and Rawalpindi respectively. Fifty (n=50) chickpea seed samples were analyzed for nutritional profile.
The comparative nutritional profile of chickpea seed samples of 3 districts of Punjab showed that high protein contents were
found in Chakwal district (23.47%) followed by Rawalpindi district (22.96%) and Khushab district (22.79%) respectively.
The moisture content of seed samples of Chakwal (6.40%) was higher followed by Khushab (5.90%) and Rawalpindi
(5.52%). The higher crude fiber content was found in samples collected from Khushab district (5.89%) followed by
Chakwal (5.89%) and Rawalpindi district (5.83%). Similarly, crude fat of seed samples of Rawalpindi district was 5.41%
followed by Chakwal (5.09%) and Khushab (5.00%). While crude ash content of district Rawalpindi was higher i.e. 3.16%
followed by Khushab (3.12%) and Chakwal (2.99%) respectively.
PPBG 14

EFFECT OF EXOGENOUS APPLICATION OF NATURAL AND SYNTHETIC GROWTH

ENHANCERS ON QUANTITATIVE AND QUALITATIVE ATTRIBUTES
OF TOMATO (LYCOPERSICUM ESCULENTUM)
AZRA YASMEEN1, SHAHZAD MAQSOOD1, AHMED BASRA1, MUHAMMAD MANSOOR JAVAID1,
HAFEEZ-UR-REHMAN2, ABDUL WAHID4 AND NAZIM HUSSAIN1
1

Department of Agronomy, University College of Agriculture, Bahauddin Zakariya University, Multan

2
Department of Crop Physiology, University of Agriculture Faisalabad, 38040, Pakistan
3
Department of Agronomy, University College of Agriculture, University of Sargodha, 40100, Pakistan
4
Department of Botany, University of Agriculture Faisalabad, 38040, Pakistan
* Corresponding authors email: [email protected]
In pot experiment, soil and foliar application of a natural and synthetic plant growth enhancer i.e. moringa leaf
extract (MLE) and benzyl amino purine (BAP), respectively was estimated for growth and development of tomato. The
treatments were control (distilled water), MLE0 (100 % pure MLE), MLE10, MLE20 and 30 (10, 20 and 30 times
diluted MLE, respectively) and BAP (50 mg L-1). The foliar application of BAP and MLE30 enhanced the number of
vegetative and flowering branches of tomato as compared to other treatments. Whereas, soil application of MLE 30 and
MLE 20 enhanced the vegetative and flowering branches, respectively. Similarly, foliar application of BAP and MLE30
showed maximum flower number and heaviest fruit weight per plant. Exogenous application of BAP or MLE 30
comparatively increased chlorophyll a and decreased chlorophyll b contents. Total soluble protein was maximum with
BAP, MLE 20 and MLE 30 either soil or foliar application. In comparison, foliar spray of MLE 30 recorded maximum
contents of enzymatic antioxidants superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), total phenolics and
fruit lycopene contents in tomato. Foliar application of MLE or BAP was more effective for tomato growth and
development as compared to soil applied.
PPBG 15

GAS EXCHANGE ATTRIBUTES CAN BE VALUABLE SELECTION CRITERIA FOR

SALINITY TOLERANCE IN CANOLA CULTIVARS (BRASSICA NAPUS L.)
ERUM MUKHTAR, EJAZ HUSSAIN SIDDIQI, KHIZAR HAYAT BHATTI*,
KHALID NAWAZ AND KHALID HUSSAIN
Department of Botany, Institute of Chemical and Biological Sciences, University of Gujrat, Gujrat-50700, Pakistan.
*
Corresponding authors e-mail: [email protected]
Present study was carried out to access inter-cultivar variation for salt tolerance in canola (Brassica napus L.) by
using photosynthetic attributes including photosynthetic pigments as selection criteria. Four cultivars of canola viz.
Oscar, Ac Excel, Cyclone and Dunkled were screened at 120 mM NaCl at vegetative stage. Salt stress reduced
photosynthetic rate (A), transpiration rate (E), stomatal conductance (gs), sub-stomatal CO2 (Ci) conc. at different growth

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

23

stages. Salt stress also markedly reduced chlorophyll a, chlorophyll b and total chlorophyll contents. Generally, plant
biomass declined under the salt regime of all the cultivars. Nevertheless, cultivar Dunkled had higher, Oscar and Ac
Excel intermediate while; Cyclone had lower shoot and root fresh weight under saline condition. However, cultivar
Cyclone was lower in chlorophyll a and chlorophyll b under the salt stress in contrast to Ac Excel. A significant variation
was also shown in gas exchange attributes under the stress. Photosynthetic (A) and transpiration rates (E) was higher in
Dunkled than of Oscar and Cyclone under the stress. On the basis of data, it may be concluded that fresh weight of shoot
and root had positive correlation with physiological photosynthetic rate (A) among all the four cultivars; thus,
photosynthetic rate (A) can be an effectual selection criteria for salt tolerance under salt regime.
PPBG 16

BIOCIDAL PROPERTIES OF CITRUS OILS AGAINST DENGUE

MOSQUITO AEDES ALBOPICTUS
FAISAL HAFEEZ1, WASEEM AKRAM2, UNSAR NAEEM-ULLAH3, KHURAM ZIA2
AND HAFIZ AZHAR ALI KHAN4
1

Entomological Research Institute (ERI), AARI, Faisalabad

Department of Agri. Entomology, University of Agriculture, Faisalabad
3
Water Management Office, Multan
4
Department of Entomology, University College of Agriculture, Bahauddin Zakariya University, Multan
Corresponding authors E-mail: [email protected]
2

Ethnobotanical studies provide valuable resources for the development of new product. Recently, plant based
compounds have got much attention because these are risk-free and eco-friendly. In the course of screening naturally
occurring oils from plants; especially, the potential of citrus seed and peel were investigated. Five citrus (seed and peel)
oils were tested against dengue mosquito, Aedes albopictus as larvicide, adulticide and repellent. The results showed
sweet lime (FSD) possess highest potential in all biological actions (larvicide, adulticide and repellent) followed by
sweet lime (SWL). Whereas grapefruit peel exhibited maximum potential against larval as well as adult stages of the test
insect. However, the seed oils are effective than peel oils and the oils extracted from Faisalabad (FSD) collected citrus
fruits are more potent than that of Sahiwal (SWL).
The present study indicates that the oils extracted from citrus seeds are very effective in suppressing the population
of dengue mosquito. Further, research must be needed to know the variation (potential) on the basis of fruit harvesting,
soil factors and environmental factors.
PPBG 17

DIVERSITY ANALYSIS IN CHILIES FOR AGRONOMIC TRAITS AND

TOTAL SEED PROTEIN PROFILE
FAIZA MUSHTAQ1, SHAKEEL A. JATOI2, SHAH JEHAN BALOCH3, WASEEM HASSAN SHAH1
AND SADAR UDDIN SIDDIQUI2*
1

University College of Agriculture, Sargodha University, Sargodha

Institute of Agri-biotechnology and Genetic Resources, NARC, Islamabad-45500
3
Faculty of Agriculture, Gomal University, D.I.Khan
*
Corresponding author: [email protected]

Chilli is one of the versatile and remunerative vegetable crops. Genetic diversity assessment has been made in local
and exotic chilli germplasm on the basis of morpho-agronomic traits and seed storage protein profile at Institute of Agribiotechnology and Genetic Resources, NARC, Islamabad during 2010-11. High variance for fruit yield, no of fruits per
plant, plant height, no of seed per fruit and seed diameter was observed. The correlation coefficients displayed significant
and positive correlation among yield contributing traits. Leaf length and width had a significant and positive correlation
with yield which can further be addressed for chili breeding. Fruit length showed significant correlation with pedicel
length, total fruit yield and single fruit yield and showed non significance to others. Fruit yield per plant show significant
correlation with fruit wall thickness and seed diameter. Cluster analysis placed all accessions into different groups
regardless of their collection, origin. However, genetic relationship among these accessions revealed useful information
for further in-depth studies. Contrary to morpho-agronomic traits, variability observed for seed-protein profile based on
SDS-PAGE was low among chili accessions. The preliminary information on state of genetic variability would be useful
in devising meaningful crop improvement program in chilli.

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PPBG 18

BENEFICIAL EFFECT OF VIGNA RADIATA L. TO SUBSEQUENT WHEAT CROP

FAKHRA AMBREEN, NOSHIN ILYAS*, QUDSIA BANO, REHMATULLAH QURESHI,
MUHAMMAD ARSHAD AND NAVEED IQBAL RAJA
Department of Botany, PMAS-Arid Agriculture University Rawalpindi.
*
Corresponding author: [email protected]
Crop rotation is a supportive management practice in which legumes greatly improve the growth and yield of
subsequent cereal crop particularly wheat. In this study, effects of mungbean on the following wheat crop were
determined. Experiment was done with two sets of wheat crop, one set of wheat crop was grown following mungbean
crop, while second set was grown after fallow period. Contribution of wheat crop towards soil quality was evaluated in
terms of comparison of soil physical and chemical properties before and after sowing of mungbean. Plant density, total
biomass, grain and straw yield, concentration of micro and macronutrients and phytohormones of wheat crop following
mungbean were determined. Comparison between data obtained from wheat crop after mungbean and wheat crop after
fallow period was done. Result showed increased amount of soil phosphorous from 10ppm to 38ppm, potassium from
130ppm to 300ppm, nitrate-nitogen from 12ppm to 22ppm and soil moisture content by 12% after mungbean. Increased
amount of nitrogen and other nutrients in wheat crop was also observed. This study proved that crop rotation is helpful to
increase yield of wheat crop due to increased amount of nitrogen and other micro and macro nutrients by mungbean as
compared to wheat which follow the fallow period.
PPBG 19

INHIBITION OF RHIZOCTONIA SOLANIWITH ISOTHIOCYANATES

PRODUCED BY BRASSICACEAE SPECIES
FARAH NAZ*, C.A.RAUF AND I.U.HAQUE AND G. IRSHAD
*

Department of Plant Pathology, PMAS-UAAR, Pakistan

*
Corresponding authors e-mail: [email protected]

The efficacy of bio-fumigation crops viz. Indian mustard (Brassica juncea), canola (Brassica napus), black mustard
(Brassica nigra), radish (Raphanus sativus), turnip (Brassica rapa) and cabbage (Brassica oleracea) as green manure
and soil incorporation of chopped green leaves of radish (Raphanus sativus) turnip (Brassica rapa) and cabbage
(Brassica oleracea) was evaluated to check the potential disease-suppressive activity of allelochemicals produced by
glucosinolates hydrolysis following soil incorporation in managing the soil-borne inoculum of black scurf of potato
against susceptible cultivar Desiree. Ten treatments viz. green manuring of 25 days old Indian mustard (B. juncea),
canola (B. napus), black mustard (B. nigra), radish (R. sativus), turnip (B. rapa) cabbage (B. oleracea) and soil
incorporation with chopped leaves of radish (R. sativus),turnip (B. rapa) andcabbage (B. oleracea) were employed and
compared with inoculated control. Disease was evaluated using parameters i.e. eyes germination inhibition (EGI),
sprouts killing (SK), stem canker index (SCI), stolon canker index (StCI), black scurf disease index (BSDI) and yield
reduction (YR). Green manuring of canola with 19.91% (EGI), 18.13% (SK), 28.95% (SCI), 25.10% (StCI), 28.11%
(BSDI) and 28.86% yield reduction followed by the soil incorporation of chopped leaves of turnip (B. rapa) canola with
25.58% (EGI), 21.73% (SK), 31.33% (SCI), 28.15% (StCI), 34.33% (BSDI) and 40.07% yield reduction,significantly
managed and reduced the soil-borne inoculum of R. solani and distinguishably exhibited the higher efficacy of
allelochemicals produced by glucosinolates hydrolysis following soil-incorporation as compared to the rest of the
treatments and conferred significant protection against soil-borne inoculum of the fungus when compared with the
inoculated control and rest of the treatments. The roots of turnip are generally used as vegetable and the leaves are
thrown away as litter. These leaves can be collected and incorporated in soil as compared to the green manuring of
canola (B. napus) which not only involve the cost of the seed but also an extra 25-30 days of farm practices. Increased
doze of turnip leaves may further reduce the losses caused by black scurf disease.
PPBG 20

GENOTYPIC ENVIRONMENT INTERACTION OF CASTORBEAN

GROWN OVER MULTIPLE YEARS
G. SARWAR, M. HASSAN AND A. HUSSAIN
Nuclear Institute for Agriculture and Biology (NIAB) Faisalabad Pakistan. PO Box 128
Corresponding authors e-mail: [email protected]

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This research work was performed at Nuclear Institute for Agriculture and Biology (NIAB) Faisalabad Pakistan and
castor mutants under study were obtained after treating three castor varieties viz. DS-30, C-176 and DC-15 with gamma
rays ranging from 100-500 Grey during Kharif 2003. The selection was performed in M2 generation and confirmation of
characters in the subsequent generations. Vigorous selections remained in progress during each succeeding generation
for desirable yield and yield components. Then afterward these elite selected mutant lines were evaluated from 2007 to
2010 in randomized complete block design (RCBD) in three repeats. Combined analysis of variance indicated that
genotypes (A), environment (B) and interaction between A B was highly significant. Overall mean performance of
castor mutants showed significant differences with maximum seed yield (2776 kg ha-1) estimated in mutant NIAB
Castor-2012 and minimum in standard check DS-30 (1825 kg ha-1). NIAB Castor-2012 produced 52.10% higher seed
yield followed by L-57-32-784 (39.78%) and M-7-35-1-2 (35.34%). Regarding regression coefficient (b), mutant line L
36-24-124 was more near to unity followed by NIAB Castor-2012. Standard check DS-30 not only produced poorest
seed yield but had very less regression coefficient value (0.585) which indicated that variety is most suited to special
type of environment. The maximum value of regression coefficient was estimated in mutant DC-1525421 (b=1.299)
which showed that the line is most suited for rich environment. Standard deviations to regression (Sd2) values were not
higher and less than unity and ranged from 0.107 to 0.390. Mutant line NIAB Castor-2012 had minimum value and
standard check DS-30 with highest value. Moreover, mutant line NIAB Castor-2012 had less maturity period (125 days)
as compared to 200 days of DS-30 and it can easily be harvested in one cutting operation before cultivation of wheat and
can be best fitted in wheat-castor rotation. Development of this early mutant has created the chances of adaptation of
economically potential castor as major a crop that will contribute in meeting the increasing demands of castor oil.
PPBG 21

COMPARING SALINITY TOLERANCE OF FIVE HIGH YIELDING, NON-AROMATIC

RICE (ORYZA SATIVA L.) CULTIVARS OF SINDH
G.S. CHANNA1, A.R. MAHAR1*, I. RAJPAR2, ZIA-UL-HASSAN2, J.H. UMRANI3 AND W.A. MAITLO4
1

Department of Botany, Shah Abdul Latif University, Khairpur, Pakistan

2
Department of Soil Science, Sindh Agriculture University, Tandojam.
3
Department of Crop Physiology, Sindh Agriculture University, Tandojam.
4
Date Palm Research Institute, Shah Abdul Latif University, Khairpur
*
Corresponding authors E-mail: [email protected]
Rice (Oryza sativa L.) is the second staple food crop of Pakistan. It occupies about 10% of the total cultivated area
in Pakistan. However, its growth and yield reduction is a serious issue in salt-affected areas. This study was conducted to
evaluate salinity ( NaCl ) tolerance of five local non aromatic, coarse rice cultivars viz; IR-6, IR-8, DR-82, DR-83 and
DR-92. The study comprised of a hydroponics experiment. That was conducted at the Department of Soil Science, Sindh
Agriculture University Tandojam. The pure seeds of all five cultivars were obtained from Rice Research Institute
Larkana Sindh, Pakistan. The nursery of each cultivar was initially raised in double acid washed river bed sand on
distilled water for a period of two weeks. Thereafter homogenous seedlings of each cultivar were transplanted into
plastic tubs containing 25 liters of 40 mM, 80 mM, 120 mM, and 160 mM NaCl solutions. The experiment was launched
following a two factor completely randomized design with five replications. Plants were allowed to grow up to six weeks
after transplanting. The results showed that the plants of cultivar DR-92 grown in all treatments were taller, with more
leaves and had higher dry root and shoot weights as compared to other cultivars. The better performance of cultivar DR92 was associated with less Na more K+ and higher K+/ N+a ratio than all other cultivar tested.
PPBG 22

MOLECULAR CHARACTERIZATION OF LINSEED GERMPLASM

USING DNA MARKERS
IHSAN ULLAH*, SHAHID NAZIR, MUHAMMAD ZAFFAR IQBAL AND ANAM SHAHBAZ
Agricultural Biotechnology Research Institute, AARI, Faisalabad.
Corresponding authors E-mail:[email protected]

DNA markers are useful tools for assessment of genetic diversity and phylogenetic analysis. Though co-dominant markers
like microsatellites are more useful for diversity studies, however high cost incurred on characterization of such marker has
confined its development and application to economically important crops. Random amplified polymorphic DNA (RAPD) are
influential markers for diversity studies in orphan crops like linseed where no co-dominant markers are available. The present
study was aimed for molecular characterization of linseed germplasm comprising 40 lines using RAPDs. A total of 120 RAPD
primers were employed and 82 were found informative indicating a high level of polymorphism of 68%. The amplified
products ranged from 250 to 1850bp in size. RAPD fingerprinting produced 92 markers, however, 85 unambiguous and scoreable loci were utilized for diversity analysis. Resulting similarity matrix revealed a moderate degree of mean genetic diversity
of 16% among the lines studied. In pair wise combinations, L-1 and L-4 emerged as most related with values of 91%, whereas

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26

L-20 and L-27 were found most dissimilar with similarity coefficient of 38%. Cluster analysis conducted through NTSYspc
2.2 using 85 loci, data could group 30 genotypes into five main clusters A, B, C, D and E containing 3, 5, 2, 5, 15 lines
respectively. To the best of knowledge, present study is first report on genetic diversity analysis of linseed germplasm
maintained in Pakistan using DNA markers. The information obtained here on genetic distances will be useful in future cultivar
development and molecular characterization of linseed germplasm.
PPBG 23

WHEAT GERMPLASM CHARACTERIZATION ON THE BASIS OF

MORPHOLOGICAL MARKERS
KHALIL AHMED LAGHARI AND MAHBOOB ALI SIAL*
*

Nuclear Institute of Agriculture (NIA) Tandojam

Corresponding authors e-mail: [email protected]

Germplasm, the collection of gene pool is playing a vital role to improve the crop efficiency through breeding
procedures. Breeders are always in search of old and novel genetic stock to strengthen their plant breeding programe and to
incorporate available desirable genes in the existing material. To enrich the wheat breeding and to widen the genetic
diversity, gene bank having diversified landraces, old and new local and exotic wheat varieties, advance/mutant lines
possesses various multiple genes for desired traits likewise high grain yield, stress tolerance, disease and lodging resistance
and good grain quality has always been the top priority of the breeders. Wheat breeding programme at Nuclear Institute of
Agriculture (NIA) has collection of new and old indigenous and exotic germplasm consisting of 312 genotypes possesses
multiple traits. Germplasm comprised of wild wheat species, diploid, tetraploid and hexaploid wheats. Keeping in view the
importance of this novel stock, the germplam was characterized morphologically for its better validation to facilitate the
future breeding. The data was recorded for various traits like time of ear emergence and maturity, plant height, disease and
lodging resistance, seed color and size etc. The present paper will present the detailed information of the wheat gene-pool
available at NIA, Tandojam; it will be helpful for the breeders of Pakistan and globally.
PPBG 24

GENETIC ANALYSIS FOR SOME QUANTITATIVE TRAITS IN F4 SEGREGATING

POPULATION OF WHEAT
MAHBOOB ALI SIAL1, MUHAMMAD AHMED ARAIN2*, AMANULLAH BALOCH2, KHALIL AHMED
LAGHARI1 AND SHAFI MUHAMMAD JARIKO2
1

Nuclear Institute of Agriculture (NIA), Tandojam

Department of Plant Breeding & Genetics, Sindh Agriculture University Tandojam
*
Corresponding author: [email protected]

Twenty five F4 segregating progenies of bread wheat were evaluated along with their parental lines under field
conditions. Genetic analysis was performed by calculating the genetic parameters viz., heritability (%) in broad sense,
genetic advance (G.A), genetic variance (v.g) phenotypic variance (V.p.) and the environmental variance (v.e.). Observed
means were compared by standard error. Wide genetic variation among progenies were observed for various morphological
traits viz., grain yield, 1000-kernel weight, heading time and maturity period as compared to their parental lines. Results
revealed that the seven progenies out of 25 showed the highest heritability (%) in broad sense for 1000-grain weight ranging
from 80-95% coupled the highest genetic advance (7.38 to17.34). Eight crosses combinations showed highly significant
improvement over their both the parental lines for 1000-kernel weight and yield traits. Six segregating populations showed
early maturity (106-116 days) than their parental lines. Genetic analysis of various yield components provides a valid
understanding of the segregating progenies and to know the useful parental line for future cross breeding.
PPBG 25

SPECIES DIVERSITY OF GENUS PUCCINIA PERS. (BASIDIOMYCOTA; UREDINALES)

PARASITIZING POACEOUS HOSTS FROM PAKISTAN
MALKA SABA* AND ABDUL NASIR KHALID
Department of Botany, University of the Punjab,
Quaid-e-Azam Campus, Lahore, 54950, Pakistan.
*
Corresponding authors e-mail: [email protected]

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27

Poaceous weeds are an important competitor of all economically important crop plants worldwide as well as in
Pakistan. This paper presents new records, host records and distribution of some graminicolous rust fungi from Pakistan.
During August 2009 to October 2010, different areas of Pakistan including Fairy Meadows (Northern Areas), Jalkhud,
Khaira gali, Khanspur-Ayubia, Khanspur-village, Sharan (Khyber Pakhtoonkhaw), Lawat, Leepa valley and Sharda
(Azad Jammu & Kashmir) were surveyed for rust fungi. As a result of this study, twelve (12) Puccinia species were
found parasitizing fourteen (14) different host plants belonging to Poaceae. Among these, Puccinia brachypodiiphoenicoidisvar. davisii and P. substriata var. imposita are described as new records for Pakistan. Telial stage of
Puccinia brachypodii var. arrhenatheri is described for the first time from Pakistan. In addition, Agrostis gigantea for
Pucciniaagrostidis-caninae and P. brachypodii var. poae-nemoralis, Agrostis stolonifera for P. agrostidis-caninae,
Agropyron striatum for P. graminis subsp. graminicola, Digitaria setigera for P. substriata var. imposita, Helictotrichon
virescence and Sporobolus arabicus for P. coronata var. himalensis, Koeleria macrantha for P. brachypodii var.
arrhenatheri, Piptatherum laterale for P. brachypodii-phoenicoidis var. davisii, Poa polycolea for P. brachypodii var.
poae-nemoralis and Themeda anathera for P. versicolor are new poaceous hosts for these rust fungi from Pakistan.
Puccinia cynodontis, P. melanocephala and P. sorghi have previously been reported from Pakistan but herein described
as new records for Azad Jammu & Kashmir, Pakistan. In addition to the light microscopy, Scanning Electron
Microscopy (SEM) has been utilized to magnify wall ornamentation of spores for accurate identification. This
information will ultimately lead to the use of these fungi as biocontrol of weeds to increase agricultural productivity.
PPBG 26

PHYSICO-CHEMICAL CHARACTERISTICS, PHENOLIC CONTENT AND

ANTIOXIDANT ACTIVITIES OF APPLE (MALUS DOMESTICA BORKH)
VARITIES/GENOTYPES FROM AZAD JAMMU AND KASHMIR
MAQSOOD AHMED1*, SYED MUBASHAR SABIR2, QAISAR SHABBIR 1 M.RIAZ KHAN1,
R. MOHIB MUAZZAM1 AND ABID YAQOOB 1
2

1
Department of Horticulture, Faculty of Agriculture, The University of Poonch-Rawalakot (AJK)
Department of Eastern Medicine and Surgery, Faculty of Agriculture, The University of Poonch-Rawalakot (AJK)
*
Corresponding Author: E.mail:[email protected]

Apple (Malus domestica Borkh) belongs to the Rosaceae family, is one of the dominant orchard crop of Azad
Jammu and Kashmir and abundantly grown in all temperate zone. The objective of this study was to compare the
physico-chemical properties and antioxidant activity of apple varieties/genotypes grown in temperate region of Azad
Jammu and Kashmir. To characterize the available genotypes from three locations of Poonch Division (Rawalakot, Bagh
and Sudhnotti), fourty five varieties/ genotypes were selected on the basis of plant vigour and quality of fruits. Selected
sites were visited thrice at the stage of flowering, fruit setting and fruit maturity. Regarding in situ observation for plant
morphology, selected varieties/genotype number of branches varied from 4.55(Genotype C ) to 6.66 (Starking
Delicious), average number of flowers ranged from 2567(genotype C) to 3271(Starking Delicious).Flowering duration,
average number of fruit set ,fruit set percentage ,fruit maturity percentage and days taken from fruit set to the fruit
ripening varied from 14.78(Red Delicious) to 17.22 days (Genotype A), 454.2 (Genotype B) to 582.7(Red Delicious),
15.29 (genotype B) to 19.37% (Chotta), 13.12(genotype B) to 17.06%(Spartan) and 139(Genotype C) to 155days
(Kashmiri and Royal Galla) respectively. Leaf area varied from 25.01(Spartan) to 39.98(Royal Galla). Fruit weight
varied from 122.6(Spartan)to 209.2 g(Royal Galla) and for interaction 224.5 g was noted in Royal Galla at Bagh. Fruit
length and fruit diameter ranged from 4.88(genotype A) to 6.43 cm (Royal Galla) and 5.16(Genotype A) to 6.80 cm
(Royal Galla) respectively. pH, total soluble solids (TSS), vitamin C content, acidity%, reducing sugar, non reducing
sugar and dry matter content varied from 3.62(Ammri) to 4.0(Royal Galla),12.06(Starking)to 14.47 Brixo
(Ammri),12.33(Banki) to 14.01 mg/100g(Royal Galla),0.25(Genotype C) to 0.36 %(Ammri), 7.50(Golden Delicious) to
8.64 mg/100g (Kala Kalu), 1.89 (Kala Kalu) to 2.34mg/100g (Royal Galla) and 14.05 (Starking) to 16.47% (Ammri)
respectively. The interaction values for Vitamin C 14.35 mg/100 g was noted in Red Delicious at Sudhnotti. The values
of total phenolic content, total antioxidant activity and total monomeric anthocyanin content ranged from2.20 (Royal
Galla) to8.15 mg/g(Kala Kalu),81.72 (Banki) to 165.90 g/ml (Genotype A) and (Genotype A) and 4.86(Spartan)to
7.40mg/L(Genotype B) respectively . Interaction effect for total phenolic content, total antioxidant activity and total
monomeric anthocyanin content was seen in 12.10 mg/g in Ammri at Rawalakot, 211.30 mg/ml in Genotype B at
Sudhnotti and 8.83mg/L in Genotype B at Bagh location respectively. The result suggested that genotype is the main
factor that determine the composition of bioactive compounds in apples and this provides important information on how
to make the best use of the apple varieties investigated. The evaluation of these genotypes was not only provide
important information regarding nutritional status of genotypes for the commercial exploitation but also navigate the
biodiversity exist in Malus germplasm in this region.

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PPBG 27

PHYSIOLOGICAL AND FORAGE PERFORMANCE OF HYBRID VS COMPOSITE

BRASSICA VARIETIES UNDER RAINFED CONDITIONS
MUBASHIR HUSSAIN, MUHAMMAD ANSAR*, SAFDAR ALI, AHMAD SHER,
AND HAFIZ MUHAMMAD JHANZAB
Department of Agronomy, PMAS-Arid Agriculture University Rawalpindi, Pakistan
*
Corresponding authors email: [email protected]
Fodder crops are grown in rainfed areas on very limited area due to small land holdings and competition area wise with
food and cash crops. Livestock is an alternate source of economic stability and livelihood in dry land farming conditions
especially when crop failed due to harsh climate. Development of livestock in marginal areas and drier is not possible without
nutritional forage. Keeping in view this background, a field experiment was carried out at University Research Farm (URF),
Chakwal Road Rawalpindi during winter 2010-11 to evaluate physiological and forage response of hybrid vs composite
brassica varieties under rainfed conditions. Experiment consisting of one composite (Chakwal Sarson) and three hybrid (Hyola401, Omega-1 and Omega-3) of brassica was arranged in randomized complete block design with three replications. The
results regarding physiological and forage yield performance showed significant difference among each-others. Maximum
values of physiological parameters such as photosynthetic rate (4.3 mol m-2 s-1), transpiration rate (0.9 mol m-2 s-1), stomatal
conductance (0.21 mol m-2 s-1), stomatal resistance (45.8 m2 s mol-1) and photosynthetic active radiation (1536.2 mol m-2 d-1)
were recorded by hybrid brassica Hyola-401 followed by Omega-3 and Omega-1 and minimum by composite brassica cultivar
Chakwal sarson. Highest forage yield of 70 t ha-1 can be obtained for hybrid brassica Hyola-401 as a result of better
performance. On the basis of these investigations, it is concluded that Hyola-401 hybrid brassica can be adopted for more
forage production under rainfed conditions. The obtained results will be presented in the conference.
PPBG 28

CHEMICAL COMPOSITION AND SENSORY EVALUATION OF TEA (CAMELLIA

SINENSIS) COMMERCIALIZED IN PAKISTAN
MUHAMMAD ADNAN1, ASIF AHMAD1, ANWAAR AHMED1, NAUMAN KHALID2, IMRAN HAYAT1
AND IFTIKHAR AHMED3*
1

Department of Food Technology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Graduate School of Agriculture and Life Sciences, Department of Global Agriculture, University of Tokyo, Japan
3
National Institute for Genomics and Advanced Biotechnology, NARC, Islamabad, Pakistan
*
Corresponding authors e-mail: [email protected]

The quality of black and green commercial tea samples was accessed by physicochemical analysis for mineral
composition and sensory evaluation. Significant variations in physicochemical and organoleptic parameters observed. The
moisture, protein, fat, crude fiber, water extracts and ash contents of the commercial tea samples were found to be in the range
of 2.467.47 , 0.871.141, 0.942.15, 11.2317.21, 32.3453.61, and 3.295.86%, respectively, whereas caffeine and catechin
were found in the range of 2.344.33% and 07.44%, respectively. The highest percentage of moisture, protein, fat, and crude
fiber contents were observed in green tea samples while highest percentage of ash and water extracts were observed in black
tea samples. Calcium, magnesium, sodium, potassium and manganese were found to be in the range of 1.473.84, 2.975.66,
0.391.83, 3.014.00, 1.092.43 mg/l, respectively, with maximum amounts found in green tea as compared to black tea.
PPBG 29

ENHANCING PHOSPHORUS USE EFFICIENCY IN CEREALS BY PHOSPHORIC ACID

APPLICATION IN ALKALINE CALCAREOUS SOILS
MUHAMMAD AKHTAR*, ASIF NAEEM, FAQIR HUSSAIN, M. YASIN ASHRAF, JAVED AKHTER
AND K. MAHMOOD
Nuclear Institute for Agriculture and Biology, Jhang Road Faisalabad
*
Corresponding authors e-mail: [email protected]
Major causes of low P use efficiency are the inefficient existing phosphate fertilizers and inappropriate methods of
their application. Studies were conducted to investigate the impact of premixing, subsurface placement and fertigation of
P fertilizers (phosphoric acid, DAP and TSP) on P use efficiency and yield of wheat, maize and rice. In general, lower P
rates (22 & 44 kg P2O5 ha-1) in wheat exhibited higher P use efficiency. Phosphoric acid was found a better P source
while its placement in wheat & maize was a better method of application. Relative efficiency of the P sources in wheat &

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29

maize was generally in the order: phosphoric acid > TSP > DAP and performance of methods of application in wheat &
maize was in the order: placement 5 cm below seed > fertigation at first irrigation > premixing at sowing. Phosphorus
uptake was influenced significantly by different rates of P fertilizer application. In general, maximum P uptake was
observed in case of the highest rate of phosphoric acid (88 kg ha-1 P2O5) applied as subsurface placement. Phosphorus
treatments also improved N uptake by grain. The post harvest soil analysis showed no adverse effect of phosphoric acid
addition on soil properties (pH, EC, CaCO3, organic matter) even on addition of its highest rate. Overall, the studies
revealed phosphoric acid as a better and cheaper alternative to DAP/TSP for wheat, maize and rice production.
PPBG 30

EFFECT OF BORON AND GA3 ON THE FLOWERING AND FRUIT SETTING

IN OLIVE (OLEAEUROPEAE L.) CV. USLU.
MUHAMMAD ALI, MUKHTAR AHMAD, NAVEED ANJAM, HAFEEZ-UR-REHMAN AND SUDHEER TARIQ

Fruit Crops Program, Horticultural Research Institute, NARC. Islamabad

Corresponding authors e-mail: [email protected]
A field experiment was carried out to evaluate the efficiency of Boron and Gibberellic Accid (GA3) on fruit setting
phenomenon in Olive cultivar Uslu at the Experimental Olive fruit orchard at NARC, HRI during 2010. The Olive plants
were sprayed with 3 levels of Boron @ 0.03, 0.06 and 0.09 %) and (GA3) was also sprayed in 3 levels (0.20ppm,
0.25ppm and 0.30ppm) after flowering after flowering opening. The results showed that maximum flowering (No. 778)
with the application of Boron treatment @ 0.09%, while GA3 maximum flowering (No. 189) with 30 ppm treatment.
Maximum fruit setting (34) was recorded with Boron spray @ 0.06%, and it was followed by the application of Boron @
0.09% (28) and maximum fruit setting (21) was observed with GA3 treatment @ 20ppm.Maximum fruit set on the basis
of total number of flowers was also observed by the application of Boron treatment @ 0.06% (2.14), while GA3
maximum fruit set (1.09) was recorded with GA3 treatment @ 20ppm. On the basis of above results it may be concluded
that Boron @ 0.06% and GA3 @ 20ppm was optimum for improvement in number of flowers, fruit set, fruit size and
even fruit weight in Olive cv Uslu
PPBG 31

EFFECTS OF PHOSPHORUS LEVELS ALONE OR IN COMBINATION WITH

FARMYARD MANURE ON GROWTH, YIELD AND NUTRIENT
CONTENTS OF WHEAT IN RAINFED CONDITIONS
MUHAMMAD ALI KHAN*, MUHAMMAD ATIQULLAH KHAN AND SHAMIM-UL-SIBTAIN SHAH
Farm Operations & Services NARC, Islamabad
Corresponding e-mail: alikhan.fos @gmail.com

A field experiment was conducted at National Agricultural Research Centre, Islamabad to assess the effects of
different combinations of phosphorus (P) fertilizer with or without farmyard manure (FYM) on growth, yield and
nutrient contents of wheat during 2008-09. The soil of experimental area was sandy, clay, alkaline calcareous in nature
with low fertility and deficient in phosphorus. Single super phosphate as P source (@ 20, 30, 40 and 50 kg/ha) was
applied with or without FYM at the time of sowing. Because of different combinations of fertilizer P and FYM,
statistically significant differences in biological yield, grain yield and yield components of wheat were recorded.
Maximum wheat grain yield of 4580 kg ha-1 was obtained with the application of full fertilizer and FYM. Minimum
grain yield and biological yield were recorded with no fertilizer and only FYM application. It is suggested that P 50
kg/ha with combination FYM is recommended for growing of wheat in rainfed conditions.
PPBG 32

SPATIO-TEMPORAL CROPPING SYSTEMS TO ENHANCE HARVEST OF RESIDUAL

SOIL MOISTURE CONTENT FOR SUCCEEDING CROPS IN POTHWAR PLATEAU.
MUHAMMAD ARIF, MUHAMMAD AZIM MALIK, M. RAMZAN ANSER*,
ZAMMURAD IQBAL AHMED AND M. ANSAR
Department of Agronomy, Pir Mehr Ali Shah, Arid Agriculture University, Rawalpindi, Pakistan
*
Corresponding author Email: [email protected]

30

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

Soil moisture deficiency is the major yield limiting factor in rainfed areas of Punjab. A series of field experiments
was therefore, conducted to study the effect of ten different spatio-temporal cropping systems on the harvest of residual
soil moisture content for succeeding crops in Pothwar plateau. Three distinct agro-ecologies were selected for the study
representing high, medium and low rainfall conditions. The three year study (2003-2006) evaluated various cereal and
legume-based mono and multiple cropping systems including representative summer and winter crops of the rainfed
region i.e. Wheat, Canola, Groundnut, Maize, Oat, Sunflower and Mungbean. The results revealed that legume-based
cropping systems conserved relatively a higher percentage of volumetric soil moisture (9-13%) at all locations in
comparison to non-legume based systems indicating the need to include legumes in the conventional mono cropping
systems. The sunflower-based system with Mungbean as intercrop and groundnut-based cropping system harvested 13
and 16% higher soil moisture than maize-based system for succeeding winter crops, respectively under high rainfall
conditions. The groundnut-based systems harvested 19% higher soil moisture compared to conventional wheat based
system (fallow-wheat) in high rainfall zone. Under medium and low rainfall agro-ecologies, groundnut-based cropping
systems harvested 27% higher residual soil moisture compared to sunflower + mungbean intercropping system. The
residual soil moisture availability to a soil depth of 0-30 cm was enhanced by 5 and 7% in sunflower + mungbean
intercropping and groundnut-based cropping systems as compared to maize-based cropping systems, respectively in
high rainfall zone. Similarly, residual soil moisture harvesting was increased by 7 and 14% in groundnut-based cropping
in comparison to sunflower + mungbean intercropping and maize-based cropping systems under medium rainfall
conditions. The harvest of residual volumetric soil moisture content was also enhanced by 10 and 17% in groundnutbased crop sequences compared to sunflower + mungbean intercropping and maize based cropping systems under low
rainfall zone of Pothwar tract.
PPBG 33

PERFORMANCE OF LOCALLY DEVELOPED SUNFLOWER HYBRIDS IN PAKISTAN

MUHAMMAD ARSHAD*, M. AYUB KHAN AND MUHAMMAD AMJAD
Oilseeds Program, Crop Sciences Institute,National Agricultural Research Centre, Islamabad, Pakistan
*
Corresponding authors e-mail: [email protected]
Among locally developed sunflower hybrids, 48 hybrids were evaluated in a triplicate RCBD for nine agronomic
traits under field conditions at NARC, Islamabad during spring 2011. The data were analyzed for genetic parameters
including correlation coefficients and path analysis along with multivariate analysis. Significant differences among for
all the traits indicating the diverse nature of hybrids, whereas correlation and path analyses highlighted the importance of
seed yield contributing traits among the hybrids. The negative correlation of oil contents with seed yield had been long
been reported that is suggested to break it using novel breeding techniques. Cluster analysis based on Euclidean
dissimilarity revealed seven clusters at 25% linkage distance. Varying numbers of hybrids were grouped according to
agronomic performance that indicated the worth of cluster analysis for depicting diversity among the hybrids. The
hybrids with various mean values could be the basis for identifying the best hybrid/s for general cultivation.
PPBG 34

EFFECTS OF GAMMA () IRRADIATION ON HYDROCYANIC ACID CONTENTS IN

SUDAN GRASS (SORGHUM VULGARE VAR, SUDANESE)

MUHAMMAD ASGHAR, AFSARI S. QURESHI, FAYAZ AHMAD CHAUDHRY, ASGHAR ALI, MUKHTAR
AHMAD, ABDUL QADIR, ASGHAR ABBAS, MHAMMAD FAISAL ANWAR MALIK AND ZAFEER SAQIB
1Rangeland Research Institute, National Agricultural Research Centre, Park Road, Islamabad; 2 Department of
Biochemistry, Quaid-e-Azam University, Islamabad;.3 Department of Microbiology, Quaid-e-Azam University,
Islamabad; 4Pulses Program, Crop Sciences Institute, National Agricultural Research Centre Islamabad; 5Department
of Agronomy, PMAS-Arid Agriculture University Rawalpindi; 6Assistant Professor, College of Earth & Environmental
Sciences University of Punjab Lahore; 7Department Of Chemistry Government Degree College, Satellite Town
Rawalpindi; 8Department of Agriculture Abdul Wali Khan University,Umbaar Campus, Swabie, Pakhtoon Khawah,

Pakistan; 9Department of Environmental Sciences International Islamic University Islamabad.

*
Corresponding authors e-mail: [email protected]
Hydrocyanic acid is a strong poison. The toxicity of hydrocyanic acid is due to its great heavy metal affinity leading
to cytotoxic hypoxia. At low concentration, it is bound to other compounds. It is also present in many foods and species.
Cyanogenic glucosides occur naturally in many species of plants including Sudan grass. Cyanogenesis is the biochemical

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

31

process in which release of hydrocyanic acid (HCN) occurs when plant tissue is damaged as it occurs on ingestion in the
grazing animals. The presence of cyanogenic glucosides in Sudan grass (Sorghum vulgare var, Sudanese) is an antinutritional factor due to which the potential exists for cyanide to pre-dispose selenium deficiency in grazing animals. A
considerable genotypic variation in cyanogenesis is present in Sudan grass and it is important that highly cyanogenic
Sudan grass lines are identified to ensure that germplasm used in breeding programs does not lead to the release of
cultivars that exceed safe levels of this anti-nutritional element. An Australian procedure based on the picrate assay and
utilizes computer imagery and calibration relationships between spectral intensity (Red, Green, Blue bands) of the color
reaction on picrate paper with cyanide in standard solutions to predict hydrocyanic acid concentration in sudan grass
leaves was followed. Different doses of gamma rays were applied to develop the variability for the reduction of HCN in
Sudan grass under study. Regression Models developed through R for CN vs. Treatments of Gamma ( )irradiation
showed negative correlation which mean that with the increase in radiation dose rate the values of CN decreased
significantly (P<0.05). A few treatments i.e. T4, 6, 8 &10 proved to be very much effective in the reduction of HCN
Contents significantly (P<0.05) and destructed HCN contents substantially as compared with T1 (Control) & all other
treatments. This may be due to precursor of HCN or CN ions affected by radiation due to which production of HCN is
reduced. This could be cyanogen glucoside in the plant or seed or check on the enzymatic activity producing HCN. The
variability in these doses may be manipulated for the development of reduction or fully destruction of HCN in Sudan
grass and HCN free varieties or mutants may be produced in this way.
PPBG 35

PHYSIOLOGICAL AND BIOCHEMICAL RESPONSE OF COTTON GENOTYPES TO

DIFFERENT SALINITY LEVELS AT EARLY GROWTH STAGES
MUHAMMAD ASLAM*, AMER MAQBOOL, QAMAR UZ ZAMAN AND HARIS BILAL
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
*
Corresponding authors e-mail: [email protected]
The study was carried out to screen different cotton (Gossypium hirsutum L.) genotypes for salt tolerance by using
solution culture method. Different physiological and biochemical standards were used for screening against salt
tolerance.Thirteen cotton genotypes were evaluated for comparative performance at three NaCl concentrations viz.
control 0 ds/m, 8 ds/m and 16 ds/m. Triplicated complete randomized design was followed.Plants were harvested forty
five days after the imposition of the treatments. Ten seedlings from each accession were taken to record data. Depending
upon the response of genotypes at 8 ds/m, NIAB-111 and Russian (RL) were found most susceptible and SLH-41 and
UCD-581 were found most tolerant, whereas at 16 ds/m, NIAB-111 and MS-39 were determined as most susceptible and
Grppg-25, FH-982, TX-DOS-5-76C and Russian okra (RL) as most tolerant. According to cluster analysis, these
genotypes were grouped in 7 different clusters on the basis of their similarities regarding response to salt stress.
PPBG 36

GROWTH, YIELD AND RADIATION USE EFFICIENCY OF MAIZE HYBRIDS AT

VARYING NITROGEN LEVELS
MUHAMMAD AWAIS*, AFTAB WAJID, ASHFAQ AHMAD, TASNEEM KHALIQ, M. FARRUKH SALEEM,
USMAN BASHIR AND M. HABIB UR REHMAN
Department of Agronomy, University of Agriculture, Faisalabad, 38040, Pakistan
*
Corresponding authors e-mail:[email protected]
Nutrients have a well-known effect on growth and yield of cereals. Among various nutrients, nitrogen plays a key
role in improving maize yield but demand of crop for nitrogen varies with the cultivars. The study was conducted at
Agronomic Research Area, Department of Agronomy, University of Agriculture, Faisalabad during 2009 with objectives
of optimizing N fertilization and also to improve its utilization efficiency for promising cultivars of maize. The
experiment was laid out in randomized complete block design with split plot arrangement. Data on leaf area index (LAI),
total dry matter (TDM), fraction of intercepted radiation (Fi), accumulated radiation interception (Sa), and grain yield
were taken into account for calculating radiation use efficiency (RUE). Mean maximum value of fraction of incident
intercepted radiation (Fi) remained 96 % at 75 days after sowing (DAS) due to maximum crop canopy development. The
hybrid Monsanto-919 used radiation more efficiently (3.53 g MJ-1) for TDM accumulation and (1.42 g MJ-1) for grain
yield. Nitrogen application of 300 kg ha-1 statistically proved to be better to convert radiation into dry matter production.

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32

PPBG 37

DEVELOPING A SUSTAINABLE AND ECO-FRIENDLY WEED MANAGEMENT IN MAIZE

MUHAMMAD HAROON, MUHAMMAD SAEED*, MUDASSAR IQBAL, HAMIDA BIBI AND JEHANGHIR KHAN

Khyber Pakhtunkhwa Agricultural University Peshawar

Corresponding authors e-mail: [email protected]

To determine the effect of different mulching treatments against weeds in maize, an experiment was laid out in
simple RCB Design in summer during 2011. The mulching treatments consist of farmyard manure, chicken manure,
black plastic, white plastic, hand weeding, weed as mulch and weed check. The data were recorded on nitrogen content
in weed (%), weed frequency (%), fresh weed biomass (kg ha-1), dry weed biomass (kg ha-1), plant height (cm), fresh
biological yield (kg ha-1), harvest index (%) and cost benefit ratio. Higher nitrogen content in weeds were observed in
chicken manure which are statistically at par with farmyard manure while lower nitrogen content were recorded in hand
weeded plots. Maximum weed frequency (71.61 %) was recorded for Cyperus rotendus L., while the minimum weed
frequency was observed for Convolvulus arvensis L.(9.52). Highest fresh weed biomass was recorded in weedy check
(389.90 kg ha-1) and the lowest fresh weed biomass was recorded in hand weeding (35.10 kg ha-1) followed by black
plastic (92.93 Kg ha-1) and white plastic 128.70 Kg ha-1). Different mulching treatment did not significantly affected
plant height. Highest biological yield (8979 kg ha-1) was recorded in hand weeding plots however, it was statistically at
par with black plastic producing biological yield (8671.7 kg ha-1) while minimum biological yield (3800 kg ha-1) was
recorded in weedy check plots. Maximum cost-benefit ratio was observed for hand weeding plots (6.5) while minimum
cost-benefit ratio was observed for chicken manure (4.6). The results showed that it is necessary to cover the soil surface
with different materials to obtain high biological activity, retain soil moisture and to achieve a good control of weeds.
PPBG 38

IMPROVEMENT IN FLAG-LEAF AREA OF WHEAT BY ZINC APPLICATION: ITS

EFFECT ON YIELD AND GRAIN-ZINC CONCENTRATION
M. HASSAN1*, A. RASHID2* AND I. CAKMAK3
1

Plant Breeding & Genetics Division, Nuclear Institute for Agriculture & Biology (NIAB), Faisalabad, Pakistan
2
Pakistan Academy of Sciences, 3 Constitution Avenue, Islamabad, Pakistan
3
Faculty of Engineering & Natural Sciences, Sabanci University, Istanbul, Turkey
*
Corresponding authors e-mail: [email protected]

Five field experiments under uniform set of different Zn treatments were conducted in Zn-deficient soils of Pakistan.
Wheat (Triticum aestivum L., cv. Sehar-2006) was planted at each sites. Each experiment comprised of 10 treatments:
(1) RFU (Recommended Fertilizer Use, i.e., 120 kg N as urea+ 80 kg P2O5 ha-1 as triple superphosphate); (2) RFU + Soil
Zn(i.e., 50 kg ZnSO4.7H2O ha-1); (3) RFU + Foliar Zn (i.e., 0.5 % solution of ZnSO4.7H2O); (4) RFU + Soil Zn + Foliar
Zn (i.e., 50 kg ZnSO4.7H2O + 0.5 % ZnSO4.7H2O ha-1); (5) RFU + OMEX- I, Foliar Zn (i.e., Omex-Type- I Foliar Zn);
(6) RFU + Omex Type-II Foliar Zn; (7) Urea-Zn (120 kg N + 80 kg P2O5 + 25 kg ZnSO4.7H2O ha-1); (8) RFU+MESZZn (Mosaic-soil Zn); (9) MESZ-Zn (Mosaic-soil Zn)+Foliar Zn (as above); and (10) Urea-Zn + Foliar Zn (as above).
The experiment was carried out as a randomized complete block design with six replications by keeping plot size of 3.6
x 9.6 m . Maximum increase in flag-leaf area 21.69 cm-2 was achieved by treatment (7) Urea-Zn; however, increase in
flag-leaf area with treatments: (9) MESZ-Zn + Foliar Zn , (6) RFU + OMEX-II Foliar Zn, (10) Urea-Zn + Foliar Zn, and
(5) RFU + OMEX-I Foliar Zn, was at par with maximum value. Similarly, significant incresae in grain yield was
observed by both soil application and foliar spray of Zn in all experiments (P < 0.05; Table 1). Highest yield of 5.01 t ha1
was obtained with soil applied MESZ-Zn; however, grain yields obtained with RFU + soil Zn + foliar Zn and RFU +
Omex Type-II foliar Zn were similar to the maximum yield. All zn treatments increased flag-leaf area, gain zn
concentration, and grain yield (p < 0.05); and foliar feeding was more effective than soil applied zn in zn-deficient
calcareous soils.
PPBG 39

EFFECTS OF ANISEED (PIMPINELLA ANISUM) ON OVERALL PERFORMANCE AND

MILK COMPOSITION OF DAMANI GOATS
M. IFTIKHAR1, S. AKHTER1,A. REHMAN1*, T. A. KHATTAK2, K.KALEEM2, M. SHOAIB4,
Z. R. KHALIL1, AND M. I. KHATTAK3
1

Department of Livestock Management, KP Agricultural University Peshawar, Pakistan.

2
Livestock Research and Development Station, Dera Ismail Khan, Pakistan.

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

33

Centre of Animal Nutrition, Livestock Research and Development, Peshawar. Pakistan.

4
Gomal college of Veterinary sciences, Gomal University D I Khan
*
Corresponding authors E-mail: [email protected]

Aniseed (Pimpinella Anisum,) is an annual plant, found in warm climates, of average height of 30-50cm with
grayish-brown color. Botanically aniseed belongs to family Apiaceae (Umbelliferae) from class Magnoliospida.
Medicinally this plant is used as growth promoter & immune stimulator and increased dry matter intake. An experiment
was carried out to evaluate the effect of different doses of aniseed on Feed intake (FI), Weight gain (WG), milk yield
(MY) and milk composition of Damani goats at Livestock research and Development station, Dera Ismail Khan. A total
of 20early lactating Damani goats (n=20) with homogenous characteristics were randomly grouped on the basis of three
different concentration of aniseed viz. 1, 2 and 3.0g/kg body weight supplementation, whereas one group was assigned
as control group. Data regarding FI, WG, MY and milk composition were recorded at weekly interval for 8 weeks. Milk
samples for milk composition were determined by method described by Association of Official Chemists Inc. Virginia,
USA. Results showed highly significant effect (P<0.05) of aniseed on FI, WG, MY and milk composition except ash and
Total Solid (TS). Aniseed supplementation @3g/kg body weight boost feed intake from 4.317 to 5.865kg/day, milk yield
from 1022 to 1432ml/day. Regarding milk composition significant increase in milk protein, lactose, and SNF while
significant decrease in milk fat% was observed. Further studies on the immune aspect of the said plant may be conducted
in animals and human.
PPBG 40

INFLUENCE OF CROP ROTATION AND SOWING TIME ON EPIDEMIC OF

CERCOSPORA LEAF SPOT OF PEANUT UNDER RAINFED CONDITIONS

MUHAMMAD IJAZ1*, FAYYAZ-UL-HASSAN2, M. I. HAQUE2, C.A. RAUF2 AND M. TARIQ

1

Plant Pathologist Barani Agricultural Research Institute, P.O. Box 35 Chakwal,Pakistan

2
PMAS Arid Agriculture University Rawalpindi, Pakistan
*
Corresponding authors e-mail: [email protected]

Cercospora leaf spot (CLS) is yield reducing biotic stress of peanut under rainfed conditions of crop cultivation.
Crop rotation with non-host crops and alteration in sowing time aims at to mitigate disease impact on yield and yield
parameters. Sowing of peanut crop in fallow land or in land with one or two years rotation of non-host crop of
Cercospora leaf spot delayed disease initiation from 58 to108 days and gave better yields than plots with left over seed of
previous year. Lesser infection percentage increase rates13.91, 11.72 and 7.71 in fields with one year, two year and
fallow rotation lands respectively, than in fields with left over seed (18.98%) resulted in lesser area under disease
progress curve. Under experiment on influence of planting time on Cercospora leaf spot disease initiated 23- 65 days
early in late sown crop (20th May to 30th June) than in earlier sown crop. Minimum initial infection percentage 3.64 to
5.32 was recorded in 30th April to 20th May sown crop. Minimum area under disease progress curve (audpc) 1606 2006
and minimum final infection percentage 72.18- 89.28 were recorded in 10th to 30th June sown crop. Among parameters
like days to disease initiation, initial infection percentage, final infection percentage, audpc and yield positively
correlates (r= 0.47- 0.90) with days to disease initiation and negatively with amount of initial infection percentage.
PPBG 41

EVALUATION OF BARLEY GENOTYPES FOR YIELDING ABILITY AND DROUGHT

TOLERANCE UNDER IRRIGATED AND WATER-STRESSED CONDITIONS
MUHAMMAD ILYAS KHOKHAR1*, JAIME A. TEIXEIRA DA SILVA2, M. ZAFFAR IQBAL1, SALEEM
AKHTAR1 AND MUBASHIR NIAZ3
1

Agri. Biotechnology Research Institute, Ayub Agriculture Research Institute, Faisalabad.

Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan
3
Department of Botany, Government College university, Faisalabad, Pakistan
*
Corresponding authors e-mail: [email protected]

Twelve barley genotypes based on different selection methods were evaluated for stress tolerance under drought and
irrigated conditions. The results of a correlation matrix revealed highly significant associations between Grain Yield
(Yp) and Mean Productivity (MP), Stress Tolerance Index (STI), Geometric Mean Productivity (GMP) and Yield Index
(YI) under irrigated conditions while Mean Productivity (MP), Yield Stability Index (YSI), Stress Tolerance Index
(STI), Geometric Mean Productivity (GMP) and Yield Index (YI) had a high response under stressed condition. Based

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

34

on a principal component analysis, Geometric Mean Productivity (GMP), Mean Productivity (MP) and Stress Tolerance
Index (STI) were considered to be the best parameters for selection of drought-tolerant genotypes. The 2-row barley
genotypes genotypes B-07023 and B-07021 performed better in yield response under drought conditions and were also
found to be more stable under stress conditions. Furthermore, drought stress reduced the yield of some genotypes while
others were tolerant to drought, suggesting genetic variability in this material for drought tolerance.
PPBG 42

RAPID DECLINING OF MANGO, CITRUS AND SHEESHAM TREES: AN

ENVIRONMENTAL ISSUE AND ITS CONSEQUENCES ON SOCIOECONOMICS OF
BHIMBER AREA (AK) PAKISTAN
MUHAMMAD ISHTIAQ*, TANVEER HUSSAIN AND MEHVISH MAQBOOL
Department of Botany, Mirpur University of Science and Technology, Bhimber, Azad Kashmir, Pakistan
*
Corresponding authors e-mail: [email protected]
A new and rapidly spreading disease decline of mango (Mangifer indica), citrus (Citrus esculentus) and shesham
(Dalbergia sisso) trees in Bhimber area has been investigated spatially. The rapid declining of these species population has
threatened their fruit production and timber quality in Azad Kashmir Pakistan. This loss not only shed their impacts on
socioeconomics of people of Bhimber area but also shifted the paradigm towards biotic and abiotic stress on other species in
forest ecosystem. In present study mango, citrus and shesham trees declining due to different factors are being taken into
consideration. The samples were obtained from healthy as well as diseased plants both from field and by artificially
inoculated plants. A comparison of the diseased and healthy vascular tissues (from collar portion, roots and branches)
anatomy were observed by submerging these sample pieces into water and boiling to make them soft. Several fungi
including Botryodiplodia theobromae, Ceratocystis fimbriata, Phoma spp, Aspergilus spp, Cladiosporium spp, and
Nattrasia spp were found. These pathogens invade in the xylem tissues and block the up take of mineral and nutrients,
ultimately which result the declining of the tree. These pathogens primarily infect through wounds on the trunk or branches
and rapidly invade the rays and fibers. A wood beetle is also responsible for wound of mango tree, and through this path
these pathogen enter into the plant. Phytosociological and anatomical characteristics of healthy and decline mango, citrus
and shesham trees were quite different not only from each other but also int. Due to fungal infection the vessel diameter,
vessel length, ray height, ray width, fiber length and lumen diameter of decline tree was larger as compare to that of healthy
samples. The present study will be help full for the plant pathologist to find out the solution of the declining. The current
study will be useful to assess the biodiversity loss and their consequences on the socioeconomics of society.
PPBG 43

GENETIC VARIABILITY IN PROLINE AND ITS RELATIONSHIP WITH YIELD AND

YIELD PARAMETERS OF COTTON CULTIVARS GROWN UNDER
WATER STRESS CONDITIONS
MUHAMMAD KASHIF SHAHZAD SARWAR1*, MEHBOOB-UR-RAHMAN1, M. YASIN ASHRAF2,
MUHAMMAD ZAMAN1 AND YUSUF ZAFAR3
1
National Institute for Biotechnology & Genetic Engineering, Faisalabad, Pakistan
Plant Stress Physiology and Biochemistry Lab, Nuclear Institute for Agriculture & Biology, Faisalabad, Pakistan
3
Agriculture and Biotechnology Wing, Biosciences Division, Pakistan Atomic Energy Commission, Islamabad, Pakistan
*
Corresponding authors e-mail: [email protected]
2

Water scarcity is an important factor limiting cotton production worldwide particularly in Pakistan. To identify
drought tolerant genotypes, it is vital to understand their genetic variation for different biochemical traits under water
limited conditions. In the present study, cotton (Gossypium hirsutum L.) accessions were evaluated under two irrigation
regimes viz. well watered (W1) and limited water (W2) conditions. Before physiological maturity, cotton leaves were
collected and analyzed for praline accumulation. At maturity, data regarding yield and yield parameters were recorded.
Significant reduction in case of proline, and yield parameters was observed under W2 condition in all the genotypes;
however, Correlation between the yield parameters ofcotton and proline was determined. This study shows that proline
was regulated genetically and environmentally in the tested cotton genotypes. It was concluded that proline can be used
as biochemical marker for screening cotton germplasm for drought tolerance as well as for evolving high yielding
drought tolerant varieties of this crop. The findings are useful in bridging plant biochemistry and molecular biology for
identifying and selecting genes involved in conferring drought tolerance in cotton.

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PPBG 44

ANALYSIS OF COMBINING ABILITY STATUS AMONG PARENTS AND HYBRIDS IN TOMATO

MUHAMMAD MAZHAR HUSSAIN*1, ASIF SAEED1, ASIF ALI KHAN1, AMIR SHAKEEL1,
NADEEM SAEED2 AND M. MASSUB TEHSEEN1
1

Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

Department of Mathematics and statistics, University of Agriculture, Faisalabad, Pakistan
Corresponding authors e-mail: [email protected]

+A set of 12 F1s developed by line tester mating design were studied to investigate the nature and relative
contribution of general combining ability, specific combing ability and simple correlation of selected tomato cultivars for
yield and yield related traits in tomato. Data recorded for different traits as plant height, fruit length, fruit width, fruit
weight, number of fruits per cluster, number of flowers per cluster, days to first picking, number of branches per plant
and fruit setting percentage showed that variability among crosses was mainly due to contribution of lines and line
tester interaction. Correlation studies showed that fruit yield per plant has strong positive correlation and significant
values (p0.01) for branches per plant and fruit setting per percentage while plant height had negative significant
correlation for number of flowers per cluster. For specific combing ability, cross combinations CLN-2001 BL-1175,
BL-1173 BL-1175, CLN-2001A BL-1176 and cross BL-1173 BL-1176 showed positive specific combining ability
effects for fruit yield per plant while lines CLN-2001A and BL-1174 were found as good general combiners in term of
fruit yield per plant. Among testers, BL-1176 and 17895 showed good general combining ability effects for fruit yield
per plant. So these lines may be useful as parents in a local varietal development program.
PPBG 45

SCREENING OF FORAGE GENOTYPES FOR SALT TOLERANCE AND FORAGE

PRODUCTION IN SALINE ENVIRONMENTS IN PAKISTAN
MUHAMMAD RIZWAN*, K. MAHMOOD, M.Y. ASHRAF, M.I. CHUGHTAI, A.R. AWAN AND M. SALEEM
Nuclear Institute for Agriculture and Biology (NIAB), P.O. Box No. 128, Faisalabad, Pakistan
*
Corresponding authors e-mail: [email protected]
In Pakistan, about 6.68 Mha of arable land is affected by salinity and/or sodicity to varying degrees. The saltaffected areas face the problems of both shortage and quality of fodder to meet feeding/dietary requirements of livestock
animals. The objective of present work was to screen forage germplasm for improving productivity of saline marginal
lands with brackish water. A large number of varieties provided by ICBA including summer forage species, Sorghum
(25) and Pearl millet (30), and winter forages species Barley (25), Brassica (4), Alfafa (8) and Fodder beet (7) were
screened under saline field conditions. Local forages, Berseem (4) and Oat (4), were also included in the trials. Forage
production was determined at three salinity levels in the field, i.e. low (3-8 dS m-1), medium (8-12 dS m-1) and high (1218 dS m-1). Winter forages were irrigated with tube-well water (EC 3.9 dS m-1, pH 8.0, SAR 24.9 and RSC 7.6 meq l-1)
while summer forages were generally irrigated with tube-well water but occasionally with canal water. Further
evaluation of selected forage species/varieties was done at farmers fields under low (ECe 5 dS m-1) and medium (ECe10
dS m-1) salinity. Biomass yield was gradually reduced with increase in salinity of the soil. Forage production of most of
the tested species may not be economical at high salinity levels above 12 dS m-1. Most of the barley, Brassica,Sorghum
and Pearl millet varieties provided by ICBA were either superior or similar in dry matter production as compared to local
varieties. Among winter forages, Brassica, oat and barley had higher yields than others under low and medium salinity,
however, at high salinity the differences between species were narrow. Pearl millet had outstandingly higher yield than
Sorghum particularly under low salinity. Barley cultivars 116/2A, 91/2A 57/2D and 59/3A, Brassica varieties Hobson,
Hyola-60 and Hyola-43, Fodder beet variety Maestro, Oat F-311 and Berseem Faisalabad Super late produced maximum
dry matter at low to medium salinity. Two local varieties of alfalfa: AARI-lucerne and Sargodha-lucerne proved superior
to other local or ICBA provided varieties. Sorghum genotypes ICSR-9302-1, ICSR-172, ICSV-745 and Speed Feed and
Pearl millet varieties, IP-22269, IP-6111 and IP-19586 produced higher biomass yield over a range of salinity levels.
Based on further 2 year data from testing on farmers fields, the ICBA provided germplasm (Barley, Sorghum and
Pearl millet) gave higher forage yields, particularly under low and medium salinity (up to 12 dS m-1), as compared to
local genotypes. Cultivation of selected forages resulted in 40-50% increase in forage production and thus carrying
capacity of saline marginal lands (12 dS m-1) on private farms. The work conducted under this study has confirmed the
potential for use of saline lands and brackish water for forage production on sustained basis.

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PPBG 46

VARIABILITY ASSESSED IN RED ROT RESISTANT SOMACLONES OF SUGARCANE

GENOTYPE S97US297 IN R1 AND R2 GENERATIONS
MUHAMMAD TASDIQ HUSSAIN SHAHID1*, FAROOQ AHMAD KHAN2,
F.M. AZHAR2, BILQUEES FATIMA3 AND MUHAMMAD ASLAM2
1

Agri. Biotechnology Research Institute (AARI), Faisalabad, Pakistan

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan
3
Department of Horticulture, University of Agriculture, Faisalabad, Pakistan
*
Corresponding authors e-mail:[email protected]

Sugarcane globally is a major source of commercial sugar. In world production Pakistan is 5th in ranking Breeding
sugarcane under climatic conditions prevailing in Pakistan is very difficult. Somaclonal variation provides an alternate to
generate variability in the existing genotypes. Fifteen red rot resistant entries (somaclones) selected from R0 generation
were studied in R1 and R2 generations in three replications using randomized complete block design. Analysis of variance
and different genetic parameters were calculated. Cluster analysis was done using Wards method. Somaclones showed
highly significant difference in all the agronomic and quality traits except pol and purity in R1 generation. Whereas in R2
generation significant and highly significant differences were observed in all the parameters studied. A wider variation was
observed in all the characters especially in morphological traits. Variance computed in the table indicated that maximum
variation was found for leaf area, plant height, cane height, number of millable canes and cane yield. The genotypic
variances for the said characters were 897.34, 142.23, 87.58, 75.65 and 59.57 respectively. Genotypic variance followed
the same trend as of phenotypic variance for all the characters. High genotypic coefficient of variability as well as
phenotypic coefficient of variability percentage was observed for number of internodes, number of millable canes, cane
yield and cane weight. Heritability estimates in broad sense were relatively high for almost all the traits studied
especially cane thickness, number of millable canes, cane yield, cane weight and leaf area in R1 generation whereas in R2
generation only plant height, number of internodes and leaf area showed high heritability. This indicated the presence of
additive type of gene action in the expression of these parameters. In both the generations somaclones divided into
three clusters. Formation of clusters indicated the presence of similarities with in cluster and divergence with the
somaclones present in the other clusters. Two years studies authenticate that these are the somaclones which are on
the basis of parameters studied close to each other and with the parent, however somaclones are resistant to red rot
and parent is susceptible to red rot disease.
PPBG 47

EFFECT OF FOLIAR FEEDING ON BIOFORTIFICATION OF WHEAT

MUHAMMAD YASEEN*, MUHAMMAD ARSHAD AND WAZIR AHMED
Institute of Soil & Environmental Sciences, University of Agriculture, Faisalabad
*
Corresponding authors e-mail: [email protected]
Wheat grown on calcareous or alkaline soils produces grain/ flour deficient in micronutrients particularly Zn and Fe
etc. Moreover, in recent years, production of wheat is going down due to imbalanced use of nutrients particularly
micronutrients. Soil application of micronutrients merely meets the demand of wheat for micronutrients due to fixation
in soils. However, alternate and reliable approach for biofortification of wheat is foliar application of micronutrients. A
mixture of six micronutrients was formulated by adding an appetizer that improves the uptake of nutrients from foliage
surface. A series of experiments were conducted on former fields in the district of Toba Tek Singh with collaboration of
Endowment Fund Secretariat, University of Agriculture, Faisalabad to evaluate the effect of foliar application of
multinutrients plus soil applied fertilizers on growth and yield of wheat. Multinutrients were sprayed @ 500, 1000, 1500
ml acre-1 in the presence of soil applied recommended dose of NPK fertilizers while NPK fertilizer alone was kept as
control. Results revealed that foliar application of multinutrients significantly affected yielding parameters of wheat that
ultimately caused increase in wheat yield per hectare. Foliar application of multinutrients also affected biological yield of
wheat and improved wheat grain production by 25-30% compared to soil applied fertilizers. Moreover, foliar feeding
increase uptake of Fe, Zn, Cu, B and Mn by wheat grains by 37, 64, 82, 63 and 52% over grains produced by
traditionally used fertilizers, respectively. Similarly, significant increase in benefit-cost ratio and value-cost ratio makes
foliar application of multinutrients economically more cost effective and more reliable supplementary approach for
farmers. Comparison of all tested rates showed that application of foliar spray of multinutrients at the rate of 1000 ml
acre-1 in two splits must be applied along with soil applied NPK fertilizers to get maximum grain yield of wheat.

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

37

PPBG 48

DATE PALM CULTIVATION IN SINDH: CURRENT STATUS AND FUTURE PROSPECTS

MUSHTAQUE AHMED JATOI*
Date Palm Research Institute, Shah Abdul Latif University, Khairpur, Sindh, Pakistan.
*
Corresponding authors e-mail: [email protected]
Date palm is one of the oldest plants cultivated by man and its origin is thought to be Mesopotamia (Iraq) or Gulf
region. However the Date seeds at least 5000 years old found in the storage godowns in the excavation of Moen-jo-Daro
storage vases indicates the presence of date palm cultivation in ancient Sindh. Khairpur is the biodiversity centre of dates
in Pakistan having more than 300 date varieties. Almost 85% of the Sindh dates produced only in Khairpur. Although,
Pakistan ranked 5th in terms of production and 3rd in terms of Export (quantity wise) in the World, but with 382 $/ton is
not even enlisted/ranked among top 20 exporting countries in terms of Unit value ($/ton). The export difficulties of dates
is mainly due to using centuries old fashioned traditional methods of pre and post harvesting, dearth of advanced dates
processing and preservation units, standardized packaging and presentation, national branding and market diversification
and establishment of modern marketing system facilities. The strength and weaknesses of date palm production and
marketing are highlighted and discussed in this study under following sections: Location and growing areas, cultivar
selection, labour versus mechanization, production and harvesting, marketing and economic viability and sustainability.
The study aimed to highlight the de facto situation of the date palm industry in Sindh and to improve the circumstances.
PPBG 49

CHEMICALS APPLICATION TO ALLEVIATE DROUGHT STRESS AND ENHANCE

PRODUCTIVITY OF BRASSICA NAPUS CV BULBUL-98 UNDER LIMITED
MOISTURE CONDITIONS
NADIA MUBARIK, AQIB IQBAL*, IJAZ ALI AND ZAHOOR AHMAD SWATI
Institute of Biotechnology and Genetic Engineering, Khyber Pukhtoonkhwa Agricultural University Peshawar, Pakistan
*
Corresponding authors e-mail: [email protected]
Drought stress greatly affects productivity and growth of plants and plays a central role in their geographical
distribution. Many studies in recent years have shown that exogenous application of various chemicals can enhance plant
stress resistance, a phenomenon known as priming. In the present experiment, Brassica napus seedlings were pre-treated
with CaCl2.2H2O and H2O2 in different concentration as foliar sprays prior to exposure to drought stress conditions. Pretreatment of plants with these chemicals resulted in a decrease in RWL (rate of water loss), quantities of H2O2 and damage
to cellular membranes. Similarly, an increase in RWC (relative water content) and chlorophyll content, and induction of
proline and specific proteins was also noted under drought stress in chemically primed seedlings. The proteins differentially
accumulated and induced after the interactive effect of these chemicals and water availability were also identified.
PPBG 50

RESPONSE OF FINE RICE (ORYZA SATIVA L.) TO APPLICATION OF VARIOUS

MICRONUTRIENTS IN DIFFERENT RICE CULTURES
NAEEM SARWAR1*, MUHAMMAD MAQSOOD1, MUHAMMAD ASHFAQ1, EHSAN ULLAH1, HAKOOMAT
ALI2, TASNEEM KHALIQ1, KHURAM MUBEEN1 AND ABDUL SATTAR1
1

Department of Agronomy, University of Agriculture, Faisalabad 38040 (Pakistan)

Department of Agronomy, University College of Agriculture, Bahuddine Zakariya University, Multan (Pakistan)
*
Corresponding authors e-mail: [email protected]

Rice cultivation is in shift from flooded to aerobic condition for water wise rice cultivation all over the world. Thus
the field experiment was conducted to explore the role of boron and zinc on growth and yield of fine rice in different rice
cultures: Aerobic rice, Flooded rice and Flooding for two weeks after transplanting and then maintained at field
capacity up to panicle initiation and again kept flooded starting from panicle initiation up to physiological maturityin
growing seasons of year 2009 and 2010. Study revealed that changing soil water contents, micronutrients dynamics in
the soil was changed which had significant effect on growth and yield of fine rice. The growth parameters (LAI, LAD,
TDM, NAR and chlorophyll contents) of rice crop were significantly hassled when it was subjected to grow in aerobic
condition. However, crop was not affected significantly when it was grown in modified rice culture and performed as
good as the normal flooded rice. Results also indicated that introduction of aerobic rice cultivation for fine rice can
increase the deficiency problem of micronutrients (B & Zn) in calcareous soils.

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PPBG 51

EXPRESSION OF DEFENSIN LIKE GENE IN PISUM SATIVUM

NAFISA1AND AMNA SHOAIB2
Institute of Agricultural Sciences, University of Punjab, Pakistan
*
Corresponding authors e-mail : [email protected]
Food contamination problems due to biotic and abiotic stresses are important issue of the modern word. The current
work was performed to investigate the influence of southern blight diseases on germination and growth rate of pea
(Pisum sativumL.)grown under abiotic stress of Cu(II).In pot trials, Cu(II) was applied @ 1.25, 2.5, 3.75 and 5.0 mg Kg-1
in 3 kg of soil filled in 12cm x 12 cm (diameter x depth) plastic pots. Inoculum of Sclerotium rolfsii was applied @ 7 g
in 3 Kgof soil. Important growth parameters were recorded in 4-week and 8-week old pea seedling. Results showed that
germination rate, shoot and root growth parameters significantly (P>0.05) reduced up to 40%, 10-35% and 10-40% in
plant treated with pathogen. There was30%, 10-30%,3-30%, 10-40% reduction in germination rate, shoot length, fresh&
dry biomass and 5-30%, 5-24%,3-50% suppression in root length, fresh & dry biomass of 30 days and 60 days old plants
in the plants. Morphological symptoms like chlorosis, crinkling and wilting of the bottomleaves stunt root and stems
were observed at both harvested time. The expression of defensin like gene (Def) were checked thorough PCR
techniques. Plant under pathogenic conditions showed higher expression of Def. Plants suffered both pathogen and metal
stress environments demonstrated that the band thickness increased with increasing metal doses.It can be concluded that
the synergistic effect of Sclerotium rolsii and copper produce more resistant in plants. So, only the recommended amount
of copper based pesticides must be used to control the disease. Excess usage of them can result in land degradation that
in turn affects food safety and crop production. Therefore, in future there should be minimum use of such soil
contaminates and research should be focused on land reclamation with eco and environment friendly plants.
PPBG 52

CATEGORIZATION OF BER GERMPLASM THROUGH BEARING AND

ASSOCIATED CHARACTERS
NASEEM SHARIF1*, NOOR- UN- NISA MEMON2 FAKHRULDIN RAZI3 AND MUHAMMAD JAFAR JASKANI4
1
Horticultural Research Institute, AARI, Faisalabad, Pakistan,
Department of Horticulture, Sindh Agriculture University, Tandojam. Pakistan,
3
Citrus Nursery Sanitation Laboratory, Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan
4
Plant Tissue Culture Cell, Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan
*
Corresponding authors e-mail: [email protected]
2

Thirty four commercial varieties of local underutilized fruit Ber were categorized to overcome the glut market
dilemma at Horticultural Research Institute AARI, Faisalabad and Jujube Research Station, Tandojam Sindh during
2010-2011. Traits viz., pedicel length (cm), calyx color, period of blooming (days), peak time of blooming (days), time
of fruit set (days), peak time of fruit set (days), time of harvest (days), and peak time of fruit harvest (days). Results
revealed significant difference among pedicel length ranged from 0.32cm (Gorh) to 0.58cm (Dehli Sufaid) for Faisalabad
germplasm and 0.25cm (Khirol Ratam) to 0.62cm (Gola Late) for Sindh germplasm while no variation was recorded for
calyx color. Varieties were classified as early, medium and late. At Faisalabad conditions Karela, Umran, Khobani were
classified as early; Fauladi, Suffon, Anokhi as mid and Dehli White, Gorh and Dilbhar as late varieties. In Sindh Gola
Early, Gola Grape, Soofi Umran and Soofi Sialkoti as first-rate early, Gola late, Gola Saffina, Gola Kararri, Soofi Gilli,
Soofi Sanghar and Soofi Local as mid whereas White Chambeli, Khirol Mukhri, Khirol Desi, and Khirol Ratam
categorized as late varieties.
PPBG 53

INHERITANCE OF OKRA LEAF TYPE, GOSSYPOL GLANDS AND

TRICHOMES IN COTTON
NAUSHERWAN NOBEL NAWAB1*, ABID HUSSAIN2, GHULAM JEELANI1, MUHAMMAD SUDHEER
TARIQ3, TAJ NASEEB KHAN1, MUHAMMAD FAROOQ1 AND ALI RAZA GURMANI4

Vegetable Crops Research Programme, Horticultural Research Institute, NARC, Islamabad, Pakistan.
2
Director Cotton Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan.
3
Horticultural Research Institute, NARC, Islamabad, Pakistan.

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39

Plant Physiology, Crop Sciences Institute, NARC, Islamabad, Pakistan.

*Corresponding author: [email protected]

Cotton growing in Pakistan is facing a great threat from the insect pest perspective, which has developed resistance
in the versatile insect pest population against the high doses of chemical sprays used in the past years. Such a growing
resistance in the cotton insect pests against the insecticide/pesticide application needs to be redressed. Insect nonpreference traits exist in nature which can reduce the insect pest population by ultimately reducing the high cost being
spent on the insecticide/pesticide application. In cotton, the traits like okra leaf type, trichomes and gossypol glands
confer significant resistance against some of the major insect pests including the sucking and chewing insect complex.
These traits are oligogenically controlled. Inheritance of these traits is simple. Crosses were made among the contrasting
traits. The segregating populations along with the parents, F1 and back crosses for each trait under study were subjected
to the inheritance studies.Okra leaf type, trichomes and gossypol glands behaved like any incompletely dominant allele.
The chi-square well fits to 1:2:1 in the F2.
PPBG 54

AGRO-PHYSIOLOGICAL RESPONSE OF SOME WHEAT

CULTIVARS TO PHOSPHORUS

NAZIM HUSSAIN 1 * , MUHAMMAD BISMILLAH KHAN 1 , RIAZ AHMAD 2 AND NIAZ AHMED 1

University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

2
University of Agriculture Faisalabad, Pakistan
*
Corresponding author: [email protected]

Agro-physiological response of some wheat cultivars to differential phosphorus fertilization were conducted at the
Experimental Farm, University College of Agriculture, Bahauddin Zakariya University, Multan (Pakistan). Experiments
were carried out for two consecutive years. The soil was a calcareous clay loam and the climate was arid in nature. Each
experiment was replicated four times in a randomized complete block design (RCBD) with split plot arrangement. Five
wheat cultivars were sown with different levels of phosphorus fertilization. Experimental treatments were as Phosphorus
levels P1= 60, P2 = 90, P3 = 120 (kg ha-1) and Wheat cultivarsV1= Uqab-2000,V2 = Inqulab-91,V3 = Bhakar-2002, V4=
Iqbal-2000, V5 = MH-97 were used.The crop was sown during the second week of November each year. The data on
various agronomic and physio-chemical characteristics of the crop were recorded following standard procedures. Various
cultivars of wheat and different P levels produced variable total dry matter and grain yield. However, grain yield in
Inqulab-91 and Iqbal-2000 was the highest and equal during both the years. Application of 120 kg ha-1 P2O5 recorded the
maximum grain, straw and biological yield ha-1. Sowing of Inqalab-91 with the application of 120 kg ha-1 P2O5
materialized into the highest net field benefit and benefit cost ratio while the lowest were recorded for Uqab-2000
fertilized with 60 kg ha-1 P2O5. Inqulab-91 and Iqbal-2000 out yielded all other cultivars, however, were at par with each
other for grain yield.
PPBG 55

EFFECT OF ZINC FERTILIZATION ON DRY MATTER PRODUCTION AND MINERAL

COMPOSITION OF IRRIGATED COTTON
NIAZ AHMED1*, MUHAMMAD ARIF ALI1, MUHAMMAD ABID1, FIAZ AHMAD2 AND NAZIM HUSSAIN1
1

University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan.

2
Central Cotton Research Institute, 60500 Multan, Pakistan.
*
Corresponding author: [email protected]

Studies on effects of Zinc (Zn) nutrition on biomass production and its concentration in irrigated cotton (Gossypium
hirsutum L.) are limited. Thus, a permanent layout [two-years field experiment (2004 and 2005)] was conducted to study
the impact of Zn fertilizer at 0.0, 5.0, 7.5, 10.0, and 12.5 kg ha-1 on the chemical composition and biomass production of
cotton plant at maturity. The soil at experimental site was alkaline (pH 8.1), calcareous (CaCO3 5.6%), and silt loam
(Typic Haplocambid)The concentration of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg),
boron (B), copper (Cu), iron (Fe), manganese (Mn), and Zn were determined in leaves, stems, burs, seed and lint.
Results showed that increasing Zn levels significantly caused the progressive accumulation of Zn in leaves and other
plant parts. The N, K, B and Zn concentrations in leaves, seed, stems, burs and lint were increased with Zn rates;
whereas concentrations of P, Ca, Mg, Cu, Fe and Mn were decreased. Increased Zn application exerted positive effect on
dry matter production could be explained by the improvement in the nutritional status of the essential macronutrients and
micronutrients.

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PPBG 56

MANGO FRUIT QUALITY, STORABILITY AND MARKETABILITY IN RESPONSE TO

PRECOOLING AND VARIOUS FUNGICIDAL TREATMENTS
OMER H. MALIK*, AMAN U. MALIK, HABAT U. ASAD, MUHAMMAD AMIN,
SYED A. RAZA AND MUHAMMAD S. KHALID
Postharvest Research and Training Centre (PRTC), Institute of Horticultural Sciences, University of Agriculture,
Faisalabad (38040), Pakistan,
*
Corresponding authors email: [email protected]
Postharvest disease incidence along the supply chains does limit the shipment potential of mango fruit around the
globe. These studies were targeted to study the impact of precooling and efficacy of a Syngenta product viz a viz Tecto
(a.i. Thiabendazole-500g/L) at different concentrations alone and in combination with Sportak (a.i. Prochloraz-450g/L)
against postharvest disease development in a commercial mango cv. Sufaid Chaunsa of Pakistan. The fruit subjected to
on-farm forced air precooling and transported under low temperature conditions (11C; 80-85%RH) had significantly
(P0.05) lesser disease and better fruit quality along with more shelf life and higher marketability index as compared to
non-precooled and traditionally transported mangoes. Moreover, non-precooled fruit had significantly higher rate of
respiration (CO2 liberation) and more weight loss as compared to precooled fruit. Among the tested fungicidal
combinations, combined application of Tecto @ 2000 ppm with sportak @0.5ml/L (Tank mix; HWT-52C; 5 min)
performed better with significant disease control (i.e. stem end rot, side rot and anthracnose) and more marketability
index during storage and ripening. The tested alone concentrations of Tecto (1000, 1500 and 2000 ppm) could not
perform well in this regard. However, the effect of Tecto 2000 ppm was relatively better (but non-significant) in terms
of marketable fruit followed by Tecto-1500ppm than other Tecto concentrations, carbendazim and mancozeb. This paper
discusses the effect of precooling and different fungicidal treatments (alone or in combination) on postharvest disease
development, marketability, shelflife and physico-chemical quality of Sufaid Chaunsa mangoes.
PPBG 57

EFFECTS OF GAMMA IRRADIATION ON SEEDLING STAGE OF

DIFFERENT WHEAT VARIETIES
QURAT-UL-AIN BUGHIO1*, PERVISH SIAL1, MAHBOOB ALI SIAL2 AND KHALIL AHMED LAGHARI2
1

Department of Plant Breeding & Genetics, Sindh Agriculture University Tandojam

2
Nuclear Institute of Agriculture (NIA), Tandojam
*
Corresponding author: [email protected]

Induced-mutation is playing a significant role in crop improvements. To determine the effects of various doses of
gamma on seed germination and some other growth traits, the experiment was conducted under laboratory conditions.
The dry seeds of two widely adapted commercial wheat varieties Khirman and TD-1 along with two advance lines NIASaarang (drought tolerant) and ESW-9525 (salt-tolerant) were irradiated using 60Co gamma rays with different doses
viz., 150 Gy, 200 Gy, 250 Gy and 300Gy. Data were recorded on germination (%), shoot length (cm), root length, shoot
fresh weight (mg) and root fresh weight was statistically analyzed. All the measured seedling characters showed
significant (P>0.05) reduction with the increase of gamma-irradiation dose i.e. 250 and 300 Gy respectively; however,
germination delayed due to higher doses of gamma rays. Genotypes showed differential response to various doses.
Genotypes Khirman and ESW-9525 were found more sensitive as compared to NIA-Saarnag and T.D-1. Khirman and
NIA-Saarang were more responsive to higher doses as compared to T.D-1 and ESW-9525 for shoot length. The
information generated through present studies will be useful for the breeders to select mutant plants with desirable traits
in successive mutation generations and to determine the suitable dose to obtain more genetic variability.
PPBG 58

NITROGEN AND RADIATION USE EFFICIENCIES OF C4 SUMMER CEREALS TO

NITROGEN SPLIT APPLICATION
S. AHMAD1*, H. ALI1, S.U. KHAN1, U. FAROOQ1, M. ZIA-UL-HAQ1 AND M.A. KHAN2
1

Department of Agronomy, Bahauddin Zakariya University, Multan-60800, Pakistan

2
Executive District Officer Agriculture, Chiniot-35400, Punjab, Pakistan
*
Corresponding authors email: [email protected]

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

41

We studied the response of three C4 summer cereal crops i.e. maize (Zea mays L.), millet (Pennicetum americanum L.)
and sorghum (Sorghum bicolor L.) cultivars to split nitrogen for nitrogen and radiation use efficiencies (N&RUEs) under
irrigated arid environment. Field studies were conducted at the Agronomic Research Area, Bahauddin Zakariya University
Multan, Pakistan during the summer seasons 2010 and 2011. The treatments consisted of three cultivars each of three summer
cereal crops i.e. maize (V1 = Sahiwal-2002, V2 = MMRI Yellow and V3 = Pearl), millet (V1 = YBS-93, V2 = YBS-89 and V3 =
18 BY) and sorghum (V1 = Jumbo, V2 = YSS-98 and V3 = YSS-9) and five nitrogenous splits i.e., NS0 = control, NS1 = whole
N at sowing, NS2 = N at sowing + N at 1st irrigation, NS3 = 1/3 N at sowing + 1/3 N at 1st irrigation + 1/3 N at 2nd
irrigation and NS4 = N at sowing + N at 1st irrigation + N at 2nd irrigation + N at 3rd irrigation. Among cultivars Pearl,
18 BY and Jumbo produced the highest total dry matter (TDM) and grain yield, respectively. Agronomic nitrogen use
efficiency (ANUE) for maize, millet and sorghum varied from 15.38 to 33.47 kg kg-1. Among N splits, NS3 recorded the
highest ANUE of 19.11 to 38.05 kg kg-1 for maize, millet and sorghum crops, respectively. Economic nitrogen use efficiency
(ENUE) for maize, millet and sorghum crops ranged from 5.04 to 9.12 $ kg-1. Radiation use efficiency (RUE) for C4 summer
cereal crops ranged from 2.11 g MJ-1 to 3.47 g MJ-1. However, higher RUE values were observed in case of NS3 split and the
lowest were found in case of NS4 split for maize, millet and sorghum crops.
PPBG 59

EFFECT OF INTERCROPPING ON ROOT ROT DISEASE AND AGRONOMIC

PERFORMANCE OF GROUNDNUT
S. AHMED*, N. ZAMAN AND S.A. KHAN
Institute of Agricultural Sciences, University of the Punjab, Lahore-54000, Pakistan]
*
Corresponding authors email: [email protected]
The experiment was comprised of five treatments viz. (i) Negative control (No treatment) (ii) Positive control (Fusarium
solani (FS) inoculation alone) (iii) Pennisetum glaucum + FS (iv) Sorghum bicolor + FS (v) Zea mays+ FS. Plants were free of
disease in negative control treatment. In positive control disease incidence was 98%. Intercropping of P. glaucum,S. bicolor
and Z. mays significantly reduced disease incidence to 77%, 44% and 55% respectively. In positive control disease severity
rating was in range of 0-3. P. glaucumand S. bicolor reduced the disease severity to 0-1 and 0-2 respectively. In groundnut
intercropping with Z. mays disease severity was 0-3. Plant mortality was 21% in positive control. Intercropping of P.
glaucumreduced the mortality to 21% where as S. bicolor and Z. mays reduced the plant mortality to 17% and 18%
respectively. All treatments significantly affected groundnut yield. In negative control yield was highest and lowest in positive
control. Z. mays intercropping alleviated the negative effect of F. solani inoculation to a significant level.
PPBG 60

GENETIC DIVERSITY AS REVEALED BY RAPD ANALYSIS AMONG

THE CHICKPEA GENOTYPES
SAIEMA RASOOL1, T.O. SIDDIQI1 AND PARVAIZ AHMAD2
1

MolecularEcology Lab, Department ofBotany, JamiaHamdard,NewDelhi,India-110062

2
Department of Botany, A.S. College, 190008, University of Kashmir, Srinagar, India
*
Corresponding authors email: [email protected]

The objectives of this study were to estimate the genetic diversity and to evaluate the relationship between eight
chickpea genotypes that is the valuable source for breeding. Chickpea seed is a good source of carbohydrates and
proteins, constituting 80% of the total dry seed weight. The variable response of chickpea genotypes were characterized
by using the 15 RAPD primers. The total numbers of amplification products generated were 915, and among them 898
were found to be polymorphic. The number of amplification products ranged from 28-81 from 15 arbitrary primers. The
molecular weight of the generated bands in the present study ranged from 100 bp -2968 bp. Primers BG-30, C and OPA02 generated the maximum number of amplified products. Minimum number of 28 RAPD products were obtained with
OPA-04. 16 RAPD products were recorded as unique or species specific and resulted in 98.1% of polymorphism.
PPBG 61

COMPARISON OF WHEAT SOMACLONES WITH THEIR RESPECTIVE PARENTS

UNDER SALT STRESS CONDITIONS
SALEEM AKHTAR1 , MUBASHIR NIAZ2* , MUHAMMAD YOUNAS1 , SAJID UR REHMAN1, MUHAMMAD
ZAFFAR IQBAL1, AND TEHREEMA IFTIKHAR2
1

Agricultural biotechnology Research Institute, AARI., Faisalabad, Pakistan

Laboratory of Mycology & Biotechnology, Department of Botany, G.C. University, Faisalabad, Pakistan
*
Corresponding authors: [email protected]

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42

To induce the genetic variability in wheat for salt tolerant, ten wheat varieties i.e. Chenab-70, Punjab-76, Pasban-90,
Inqulab-91, Punjab-96, Uqab-2000, AS-2002, Bhakkar-2000 and Ufaq-2002 were used to develop salt tolerant wheat
somaclones at four salinity levels, i.e., 0, 50, 100 and 150 mM NaCl. The so developed somaclones of various wheat
genotypes along with their parents/ mother plants were sown in pots having artificially developed four levels of salinity i.e.,
0, 4, 8 and 12 dSm-1 using NaCl salt to study and compare different agronomic traits i.e. number of spikes per plant,
number of grains per spike, 100 grain weight and yield per plant among themselves. It was observed that salinity decreased
No. of spikes, spikelets, 100 grain weight and yield per plant. The maximum 100 grain weight in control treatment was
observed in AS-2002(P) and Bhakhar-2000(P) followed by Inqulab-91(S) and Punjab-76(P) as compared to other
somaclones/genotypes of wheat. Uqab-2000(S) was also at par with its parent Uqab-2002(P). At EC level of 4dSm-1,
Inqulab-91(P), Inqulab-91(S) and Bhakhar-2000 (S) were salt tolerant. At EC 8dSm-1, Bhakhar-2000(S) and LU-26S (P)
were noted as the best tolerant while at EC12dSm-1 Inqulab-91(P) was superior to all other genotypes. As for as yield per
plant is concerned somaclones Inqulab-91(P) and Bhakhar-2000(S) were found salt tolerant at 8dSm-1 in yield per plant.
PPBG 62

ASSESSMENT OF SEED-BORNE MYCOFLORA AND NUTRITIONAL PROFILE OF

LENTIL (LENS CULINARIS) GROWN IN ARID REGION
SANAM ASIF1, ABIDA AKRAM1, NAFEESA QUDSIA HANIF2*, RAHMATULLAH QURESHI1 AND ABID RIAZ1
1

Department of Botany, PMAS Arid Agriculture University Rawalpindi, Romer Labs, Pakistan Rawalpindi,
2
Romer Labs, Pakistan, Rawalpindi, Pakistan
*
Corresponding authors email: [email protected]

Lentil (Lens culinaris) is a common and the oldest domesticated pulse and has relatively higher contents of protein,
carbohydrate and calories as compared to other legumes. During present study fifty samples from different arid regions
of Punjab viz. Chakwal (n=16), Khushab (n=17) and Rawalpindi (n=17) were investigated for its mycoflora and
nutritional profile. A total of 14 fungi including A. niger, A. flavus, A. ibericus, A. sydowi, A. fumigatus,
alternariatenuissima. A. solani, Penicillium notatum, Rhizopus nigricans, culvularia clavata, F.oxysporum,
Paecilomyces variotii, Mucor and an unknown spp. were isolated from lentil seeds and 240 fungal isolates were
identified. Among all lentil samples co-occurrence of fungal isolates were ranged from 1-11 species. The dominating
fungal isolates were A. niger, A. flavus, and mucor whereas Paecilomyces variotiiand Alternaria tenussima were
observed in minimum numbers. The comparative study of mycoflora of three districts of Punjab showed that highest
incidence of fungal flora was found associated with district Khushab (84 isolates) followed by Chakwal district (76
isolates) and district Rawalpindi (74 isolates). Fifty (n=50) lentil seed samples collected from 3 arid regions of Punjab
put forth for chemical analysis. The proximate analysis of seed samples of Chakwal showed average values of moisture
(5.521.05), protein (23.461.05), fiber (4.430.80), fat (1.570.39) and ash (2.430.40) contents. The nutritional profile
of seed samples of Khushab showed average values of moisture (5.190.78), protein (23.871.16), fiber (4.380.60), fat
(1.630.45) and ash (2.240.41). Similarly, the proximate analysis of lentil seed samples of Rawalpindi showed average
values of moisture (5.810.95), protein (24.320.89), fiber (4.670.93), fat (1.550.44) and ash (2.220.48). The
comparative nutritional profile of lentil seed samples of 3 districts showed that high protein contents were found in
Rawalpindi district (24.320.89) followed by Khushab district ( 23.871.16) and Chakwal district (23.461.05)
respectively. The higher crude fiber content was found in samples collected from Rawalpindi district (4.670.93)
followed by Chakwal (4.430.80) and Khushab district (4.380.60). Similarly, crude fat (%) of seed samples of
Khushab (1.630.45) district was followed by Chakwal (1.570.39) and Rawalpindi (1.550.44). While crude ash
content of district Chakwal was higher i.e. 2.43o.40 followed by Khushab (2.24o.41) and Rawalpindi (2.220.48)
respectively.
PPBG 63

ZINC APPLICATION RESPONSES OF AUTUMN PLANTED SUNFLOWER HYBRIDS

ON PHENOLOGICAL, YIELD AND QUALITY ATTRIBUTES.
SANA ULLAH1, FAHD RASUL1, A. SATTAR1*, MUMTAZ A. CHEEMA1, M.F. SALEEM1,
M.A. WAHID1 AND A. GHAFFAR2
1

Department of Agronomy, University of Agriculture, Faisalabad, Pakistan-38060

2
AARI, Jhang road Faisalabad, Pakistan
*
Corresponding authors email: [email protected]

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

43

Zinc (Zn) as important micronutrients for plants improves growth, seed yield and quality of agronomic crops.A field
trial was conducted to investigate the impact of Zn fertilizer on phenology, yield and oil contents of autumn planted
sunflower hybrids. Experiment comprised of two hybrids viz; HYSUN-33 and S-278 placed in main plots and six Zn
levels (control, 5, 10, 15, 20 and 25 kg Zn ha1) in sub plots. Results revealed that leaf area index, head diameter, number
of achenes per head, 1000 achenes weight, achenes yield, biological yield and harvest index were significantly affected
by different zinc levels whereas plant height, days taken to 50 % flowering, heading and maturity were statistically nonsignificant. The sunflower hybrid Hysun-33 recorded maximum seed yield at the rate of 15 Zn kg ha-1. Zinc applied @
15 kg ha-1 produced maximum values for all these traits as compared to control. Maximum oil and protein percentages
were obtained at 5 kg Zn ha-1 significantly higher from control but statistically at par with other zinc levels. This study
concluded that Zinc fertilization promoted growth, yield and quality of sunflower hybrids.
PPBG 64

EFFICACY OF DIFFERENT HERBICIDES FOR WEED MANAGEMENT IN WHEAT CROP

SHAH FAHAD1*, AMJAD UR RAHMAN 1, ZIA-UR-REHMAN MASHWANI 1, 2,
ABDUL SAMAD MUMTAZ1 AND ASGHARI BANO1
1

Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan

2
Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
*
Corresponding authors email: [email protected]

The field study was conducted at Agricultural Extension Farm, Dargai, KPK during 2011 to assess the comparative
efficacy of different herbicides for weed management in wheat crop under agro-climatic conditions of Pakistan. The
experiment was laid out with four replications in randomized complete block design (RCBD). The experiment consisted of
hand weeding a weedy check and six herbicides. The post emergence application of herbicides included Aim 40 DF @ 0.02
kg a.i. ha-1, Agritop 500GL-1 @ 0.43 kg a.i. ha-1, Isoproturon 50WP @ 1 kg a.i. ha-1, Puma super 75EW @ 0.75 kg, Topik
15WP @ 0.04 kg and Buctril super 60EC @ 0.45 kg. For comparison hand weeding and weedy check were also included.
In each replication six treatments of these six herbicides were kept. The significantly affected parameters were fresh weed
biomass (kg ha-1), thousand grain weight (g), number of tillers m-2, weed control efficiency (%) and grain yield (kg ha-1).
Statistical analysis showed that maximum weed efficiency (84%) was recorded for Isoproturon 50 WP whereas minimum
value (37%) was for Aim 40 DF. Similarly maximum number of tillers m-2 (250) was recorded for Isoproturon 50 WP and
minimum (133) in weedy check. The herbicide Isoproturon 50 WP @ 1.0 kg a.i ha-1 is applied as post emergence in wheat
performed well in the entire weed and wheat crop data and exhibited effectively weed control in wheat.
PPBG 65

PRODUCTION AND QUALITY IMPROVEMENT OF LETTUCE (LACTUCA SATIVA L)

THROUGH CONTAINERIZED GROWING MEDIA UNDER COLD
GLASSHOUSE CONDITIONS
SHAHID J. BUTT*
*

Department of Horticulture, PMAS-Arid Agriculture University Rawalpindi

*
Corresponding authors email: [email protected]

Plants grown in the containers are restricted to a limited volume of the media consequently, required the careful
management in maintaining the characteristics, like organic/inorganic contents, carbon nitrogen ratio, bulk density,
percentage of air, CEC, pH value of the media and the required nutrient feed in the form of solution. The use of solid
substrates including perlite, peat, straw bale, soil-based compost greenhouse border soils were experimented to grow the
seedlings (transplants) and then planting media for the growth and development of cos lettuce (Lectuca sativa L., var.
longifolia) in the cold (unheated) glasshouse to investigate the responses of seedlings and planting media as independent
entity and also demonstrated the effect of their interaction on the crop under question. Significant results of many
physical and yield parameters (mainly including plant height, head diameter, number of days from sowing to harvesting,
total and marketable plant weight, percentage of tight and loose heads, status of tip burn etc.) were appeared indicating
that different media have vital potential for the production of seedlings and production of cos lettuce. Seedlings grown in
perlite or peat compost lead to higher yield grown in different planting media through bag culture technique. Perlite with
bags culture produced the yield at parallel with greenhouse border soil; however, it possesses many advantages over the
other containerized growing media including border soil. The alternative use of straw bale substrates over soil and other
media exhibited significant performance in growth and development of lettuce plants. Containerized soil-based compost
in bag culture, though economical but requires great care especially to avoid compactness for improving growth and
yield. For high and quality winter crop production, perlite or border soil planting media in combination with peat or
perlite seedling media can be of good match for obtaining average yield of 4.78 kg/m2.

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44

PPBG 66

DIVERSITY ANALYSIS AND RELATIONSHIPS AMONG GINGER LANDRACES

SHAKEEL A. JATOI1* AND KAZUO N. WATANABE2
1

Plant Genetic Resources Programme, National Agriculture Research Centre,Islamabad-4500, Pakistan

Gene Research Centre, Graduate School of Life and Environmental Sciences,University of Tsukuba, Japan
*
Corresponding authors email: [email protected]

Ginger (Zingiber officinale) is a valuable spice and aromatic crop having medicinal significance. To have insight into
variability pattern and relationships among ginger landraces, the current study was undertaken at University of Tsukuba,
Japan during 2005-2006. High variance for plant height, rhizome weight, rhizome thickness, sheath length, tillers per plant
and leaf length was observed in the germplasm assayed. Principal component analysis explained 80% and 90% of the total
variation by the first four PCs during the year 2005 and 2006, respectively. The representative accessions acquired from the
genebank remained dispersed in the three clusters during both the years displaying variability among accessions. The ginger
accessions collected from local markets were found interspersed among all the clusters during both years. The ginger
genotypes displayed positive and significant correlations among different quantitative traits during the two years. Plant
height, leaves per tillers, tiller thickness appeared to be of prime importance as they directly influence rhizome yield, and
provide a good indication for futures emphasis in crop improvement in ginger. No relationship between distribution pattern
and acquisition source was observed in this study. The different approaches used to analyze different aspects of ginger
provided a deep insight and indicated existence of considerable variability and diverse base which has been prioritized for
crop improvement.
PPBG 67

COMPARATIVE PERFORMANCE OF SEMI-DWARF AND DWARF WHEAT

GENOTYPES
SHAYAN SYED1, MAHBOOB ALI SIAL2* AND KHALIL AHMED LAGHARI2
1

Department of Plant Breeding & Genetics, Sindh Agriculture University Tandojam

2
Nuclear Institute of Agriculture (NIA), Tandojam
Corresponding author: [email protected]

Semi-dwarf advance wheat genotypes possesses plant height above 100 cm and dwarf genotypes with below 80 cm
plant height were evaluated for yield and various yield associated traits along with check varieties Khirman, TD-1 and
Yecora. The experiment was conducted under normal (7th November) and late sowing (1st January). Semi-dwarf
genotypes MASR-07, MASR-64, NIA-10/8, BWM-84, BWS-77 and NIA-9/5 produced higher yield than dwarf
genotypes at both planting times. However, dwarf genotypes headed and matured earlier than semi-dwarf ones and also
had higher number of grains per spike, main spike yield and 1000-grain weight. These finding gave a clue that dwarf
genotype possess many good traits to produce higher yield but they could have required different agronomic practices
such as high inputs etc. This paper will discuss the detailed study of newly developed genotypes for their different traits
and will highlight the impact of Rht genes to improve the crop productivity.
PPBG 68

PATHOGENIC VARIATIONS AND CHARACTERIZATION OF STREPTOMYCES

SCABIES ISOLATES FROM POTATO TUBERS IN RAWALPINDI DISTRICT
SHAZIA SHAHZAMAN, FARAH NAZ*, C.A.RAUF, I.U.HAQUE AND K.RIAZ
Department of Plant Pathology, PMAS-UAAR, Pakistan
*
Corresponding authors email: [email protected]
Incidence and occurrence of common scab caused by Streptomyces spp. was probed in different areas of Rawalpindi
District viz. Taxilla, Hassan abdal, Qazi abad and New City (Taxilla).Survey was carried out at the time of harvest and
tubers were examined for the presence of scab lesions differ in color from brown to black; small raised cork-like tissue in
the region of the lenticels to large deep depressed areas. Mean Incidence of disease 81.75 % was recorded which was
significantly high. Out of four varieties viz. Bartina, Desiree, Sant & Cardinal sown in four fields, progeny tubers of
Bartina variety were found to have highest CSDI (94.66%) followed by Cardinal (78%), Desiree (68%) and Sant (65%).
Pathogen was isolated from diseased potato tubers and twenty five purified isolates were named after the areas viz. Qazi
Abad (Road Side) AQAR-1, AQAR-2, AQAR-3, AQAR-4, AQAR-5 from Taxilla, BTB-1, BTB-2, BTB-3,BTB-4, BTB-5

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

45

from New City, CNC-1, CNC-2, CNC-3, CNC-4, CNC-5 from Qazi Abad (Middle Block side), DQAB-1, DQAB-2,
DQAB-3, DQAB-4, DQAB-5 from Hassan Abdal, EHA-1, EHA-2, EHA-3, EHA-4, EHA-5. All isolates were found
positive for hypersensitive reaction on tobacco plant.Ten isolates viz. AQAR-1, AQAR-2, BTB-1, BTB-2, CNC-1, CNC-2,
DQAB1, DQAB-2, EHA-1 and EHA-2 when subjected to biochemical tests were Gram positive.Microscopy (100X)
revealed that cells were filamentous ~1 m wide with average length of the cells 10-100 m. The pathogenic variability
among these Streptomyces isolates was established on cv. Bartina. All the isolates showed positive pathogenic response on
cv. Bartina as compared with control where no common scab symptoms were observed on progeny tuber. Mean common
scab disease index (CSDI) revealed by these isolates on progeny tubers however, was significantly different from each
other. Isolate CNC-2 was found most virulent followed by CNC-1, DQAB-1, DQAB-2, BTB-I, BTB-2, AQAR-1, AQAR2, EHA-2 & EHA-1. Common scab was prevalent in the field located away from the road and the intensity decreased as
moved toward the road bank from the center of the field.
PPBG 69

INVESTIGATIONS FOR COMPLEXITY OF ASCOCHYTA BLIGHT OF CHICKPEA

AND IDENTIFICATION OF RESISTANT SOURCES

SHEIKH MUHAMMAD IQBAL, ABDUL GHAFOOR1 AND IFTIKHAR AHMAD1

1

Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan;

National Agricultural Research Centre (NARC), Park Road, Islamabad, Pakistan
Corresponding author: [email protected]

Chickpea (Cicer arietinum L.) is the most important winter food legume of Pakistan cultivated rainfed in the areas
of Punjab and Khyber Pakhtoon Khawa (KPK). Blight caused by Ascochyta rabiei (Pass.) Lab. is the most devastating
disease in all the chickpea growing countries including Pakistan. The inter-relationship of disease factors including
inoculum potential, plant age, effect of leaf wetness and latent period with pathogenesis revealed a linear relationship
between disease severity and inoculum concentration. Inoculation of chickpea cultivars; Punjab 91 and C 727 at
physiological stages ranging from 2 to 12 weeks (seedling to reproductive stage) resulted that 2-weeks old seedlings
were more susceptible to disease than the adult plants that suggested screening of huge nurseries at seedling stage
followed by tolerant lines to screen under field conditions. Two to three days incubation period coupled with 1-2 sprays a
day had a pronounced effect on disease development. Disease severity index (DSI) ranged from 44 to 82% indicating
maximum at vegetative stage in the cultivar C 727, whereas the lowest DSI was on the cultivar Dasht. In another
experiment, 42 isolates of A. rabiei sampled from chickpea growing areas exhibited variation in morphological and
cultural characteristics. The virulent isolates could be categorized on the basis of clusters. The susceptible differentials
(C727, ILC 263, C 44 and CM 72) were identified but no variety was resistant that might be due to complex nature of A.
rabiei. Clustering pattern indicated the exchange of breeding material and disease cultures among the researchers or high
heterogeneity in the isolates. One experiment comprising of 448 genotypes were screened against blight at two stages
and none of the genotypes was highly resistant at any stage, whereas 46 at seedling in the greenhouse and 94 at pod
formation stage in the field were resistant. Common genotypes, which were resistant at both the stages were suggested to
be utilized in breeding programme to build disease resistance pyramids due to complex nature of Ascochyta blight.
PPBG 70

PROBING GENETIC RESISTANCE AGAINST STEM RUST IN WHEAT

VARIETIES OF PAKISTAN
SHUMAILA BANO, GHULAM MUSTAFA, SANA BASHIR, AMJAD-UR-RAHMAN
AND ABDUL SAMAD MUMTAZ*
Department of Plant Sciences, Quaid-i-Azam University
Corresponding authors e-mail: [email protected]

Stem rust is a destructive disease of wheat worldwide especially Ug99 which threatens wheat production globally. The
geographical location of Pakistan demands an efficient method for identification of resistant genetic resources, to minimize
the Ug99 threat. The present study provides information about effective resistant genes Sr22, Sr24, Sr26, Sr31 using closely
linked molecular markers. The efficiency of markers was ascertained by comparative analysis of field trials in 2011 at
Kaghan, Pakistan. Results revealed that the effective Sr26 gene is absent in respective germplasm and making it an
appealing choice for pyramiding program under MAS. Our work appraises the extensive distribution of Sr22 gene in
Pakistani wheat varieties to increase the resistance against different isolates including Ug99. Pakistani wheat commonly
bears Sr24 and Sr31 but the TTKST and TTKSK have also been shown to exhibit virulence to these genes. Although these
genes do not provide an adequate level of resistance but they are effective in different combinations to develop durable
resistance. The data provided in this work will facilitate the marker-assisted selection to develop durable resistant in short
period by reducing environmental factors. This study concentre that mutable pathogenic microbes requires continuous
research and attention. The identification of truly durable resistant genes are indispensable for wheat stem rust.

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

46

PPBG 71

MORPHO-AGRONOMIC CHARACTERIZATION OF OKRA GERMPLASM

SHUJAAT ALI1, AZHAR HUSSAIN SHAH * RAHMANI GUL2, SAFDAR HUSSAIN SHAH4,
HABIB AHMAD3 AND ZAFAR IQBAL1
3

1
Department of Botany Hazara University Mansehra Pakistan; 2Agricultural Research Institute Swat, Pakistan;
Department of Genetics, Hazara University Mansehra Pakistan; 4 IBGE, Agricultural University, Peshawar, Pakistan
*
Corresponding author: email: [email protected]

In present study 36 genetically diverse genotypes of Okra were evaluated to find out their similarities and
differences based on morphological traits for the target of genetic advancement. Among all genotypes only twenty seven
succeeded to germinate. The experiment was laid out in Randomized Complete Block Design (RCBD) with two
replications. Morphological traits of the genotypes were measured according to the coding criteria specified by the
Standard International Crop Descriptor for okra. Cluster analysis based on 14 qualitative and 14 quantitative variables
grouped all the accessions into two linkages and four clusters. A dendrogram was primed to clarify the relationship
between the genotypes. One pair of genotypes showed 38% similarity which is the least percent of similarity between
any two genotypes in Okra population. Six pairs of genotypes were fifty (50%) similar, which indicates that they are in
line at half the characters evaluated. Five genotypes of short growth habit, less fruit length and vast fruit diameter
grouped under one linkage. All the data obtained disclosed that the genotypes significantly varied from each other with
respect to all the characters studied. Okra genotypes; 000034 and 000036 produced strong branches with fruits near to
the ground, but gave high yield. These could be chosen for crossing with other genotypes; 020535 and Bemisal with
orthotropic branching (erect growth), normal plant height & low yield in order to get a best type of okra varieties having
high yield and erect growth for commercial productions.
PPBG 72

ASSESSMENT OF GENETIC DIVERSITY IN SOME ACCESSIONS OF SACCHARUM

OFFICINARUM L. BY MEANS OF MORPHOLOGICAL CHARACTERS, WITH
PRINCIPAL COMPONENT ANALYSIS (PCA).
SOHAIL AKHTER1*, FAROOQ AHMED KHAN1, MUHAMMAD ASLAM JAVED2
AND MUHAMMAD ASHFAQ ANJUM2
2

1
University of Agriculture, Faisalabad, Pakistan
Agri. Biotechnology Research Intitute, AARI, Faisalabad, Pakistan
*
Corresponding authors email: [email protected]

Ten accessions of sugarcane (Saccharum officinarum L.) viz. CP-77-400, CP-72-2086, BF-129, CPF-234, SPF-213,
HSF-242, HSF-240, Col.72, Triton and No. 46 were evaluated for twelve characters and genetic diversity investigations
were done by PCA. The experiment was conducted in triplicated randomized complete block design (RCBD). Analysis
of variance portrayed highly significant differences among the accessions for all the traits. In Principal component
analysis (PCA) no. of millable canes, no. of stools per plant and internodal distance contributed positively towards
genetic diversity and all other traits contributed negatively towards diversity, in first two PCs i.e. PC1 and PC2. Most of
the varieties showed greater extent of genetic diversity contributed by morphological traits.
PPBG 73

MICROSATELLITE (SSR) MARKERS A TOOL; FOR GENETIC DIVERSITY

ASSESSMENT AMONG SUGARCANE ACCESSIONS.
SOHAIL AKHTER1*, FAROOQ AHMED KHAN1, MUHAMMAD ASLAM JAVED2
AND MUHAMMAD ASHFAQ ANJUM2
1

University of Agriculture, Faisalabad

Agri. Biotechnology Research Intitute, AARI, Faisalabad
*
Corresponding authors email: [email protected]
2

Thirty SSR primer pairs chosen randomly from the SSR primer collection were used to detect polymorphism in 17
sugarcane accessions. A total of 62 DNA fragments were generated by the 30 primers with an average of about 2.14

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

47

bands per primer. Bands that a primer yielded in the study ranged from 1 to 4. The genetic distances for SSR data using
17 sugarcane accessions, was constructed based on Nei (1978) and relationships between accessions were portrayed
graphically in the form of a dendrogram. The value of genetic similarity ranging from 62.90% to 90.30% was observed
among the 17 sugarcane accessions. The highest genetic similarity of 90.03% was seen among genotypes S-2003-US118 and S-2003-US-312. From present study it may be concluded that SSRs markers are best tool for investigation of
genetic diversity in sugarcane.
PPBG 74

GENOTYPIC DIFFERENCES AGAINST PEG SIMULATED DROUGHT

STRESS IN TOMATO
SONIA GEORGE1, SHAKEEL A. JATOI2*, NASIR M. MINHAS1 AND SADAR UDDIN SIDDIQUI2
1

Pir Mehr Ali Shah, Arid Agriculture University Rawalpindi, Pakistan.

Plant Genetic Resources Programme, NARC, Islamabad-45500, Pakistan.
*
Corresponding authors e-mail: [email protected]

Osmotic stress simulation at 4% PEG concentration was used to investigate the effect of drought stress on ten
tomato varieties at seedling stage. The parameters recorded were germination (%), root and shoot length (cm), fresh and
dry biomass (g). Seed sowing was conducted with distilled water (control) and PEG6000 solution using between paper
method. The data recorded after ten days of stress treatment showed variable responses under induced stress and revealed
significant differences for all the parameters investigated. Regarding relative performance, Punjab Chohara was found to
be the best genotype, followed by Feston and Ratan whereas Money Maker and Tom Round were the genotypes whose
relative performance remained poor under stressed conditions. The results obtained during this study can be used for
manipulation of tomato cultivars for further crop improvement.
PPBG 75

CADMIUM INDUCED CHANGES ON GROWTH, YIELD AND ELEMENT UPTAKE OF

MAIZE AND ITS PHYTOEXTRACTION THROUGH EDTA AND CITRIC ACID
SUMERA ANWAR1*, M. YASIN ASHRAF2, MUMTAZ HUSSAIN1 AND MUHAMMAD ASHRAF1
1*

Department of Botany, University of Agriculture, Faisalabad, Pakistan

2
Nuclear Institute of Agriculture and Biology, Faisalabad, Pakistan
*
Corresponding authors email: [email protected]

The experiment was conducted to test the potential of two chelating agents (citric acid and EDTA) on the uptake of
Cd and its effect on the growth, nutrient uptake and yield of two maize varieties. Two doses of Cd (150 and 300 mg kg-1
soil) were used with a single dose (0.5 g kg-1 soil) of EDTA and citric acid each. The accumulation of Cd was enhanced
by EDTA whereas citric acid reduced its uptake. Correlation between Cd contents in shoot, root and grain versus the
ionic content (Mg, K, Ca, P, Fe, Zn) showed negative relationship for most of the ions except phosphorous which
showed positive correlation. Yield per plant and total grains weight was reduced by the application of Cd and EDTA,
however, addition of citric acid countered the toxicity of Cd. Cadmium toxicity did not affect the 100 grain weight. But
Cd toxicity reduced the number of grains, instead of grain size. The variety viz. Pak Affgoee showed more reduction in
yield under Cd stress as compared to Sahiwal-2002.
PPBG 76

GENETIC DIVERSITY IN INDIGENOUS RICE GERMPLASM FOR QUALITATIVE

AND QUANTITATIVE TRAITS
SYED MEHAR ALI SHAH* AND MUHAMMAD RIAZ
Department of Plant Breeding and Genetics, Faculty of Crop Production Sciences
KP Agricultural University, Peshawar, Pakistan
*
Corresponding authors email: [email protected]
This study was performed to assess genetic diversity in indigenous rice germplasm for morphological characteristics at
KP Agricultural University, Peshawar during 2009 rice crop growing season. Seventy-seven accessions of rice were planted
in randomized complete block design using two replications. Data were collected on 11 qualitative and 16 quantitative

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

48

traits. The genotypes showed variation for some of the qualitative traits like flag leaf angle, ligule color, awning, awn color,
panicle type and apiculus color. Highly significant (p 0.01) differences among the genotypes were observed for culm
length, flag leaf length, flag leaf width, days to 50% flowering, panicle length, primary branches panicle-1, length of
primary branches panicle-1, secondary branches panicle-1, spikelets panicle-1, grain length, grain width, 1000-grain weight,
biological yield and grain yield. Cluster analysis for qualitative traits revealed four clusters at the dissimilarity level of 6.92
while for quantitative traits nine clusters were identified at the dissimilarity level of 5.44. The first and second principal
components of principal component analysis explained 21.3 and 13.2% of the total variation, respectively. The first
component was dominated by panicle length, primary branches panicle-1, length of primary branches panicle-1 and spikelets
panicle-1. The second component was characterized by days to flowering, biological yield, grain yield and 1000-grain
weight. Entry number 10 showed the longest panicles (35.7 cm) while entry 28 displayed the highest primary branches
panicle-1 (13.5). Maximum number of secondary branches panicle-1 (55) was observed for entry number 46 while the
highest number of spikelet panicle-1 (353) was observed for entry 50. Rice entry 56 showed maximum value (39 g) for
1000-grain weight while entry 26 displayed maximum grain yield (15833 kg ha-1). On account of excellent performance for
yield and yield associated traits, entries 10, 28, 46, 50, 56 and 26 are, therefore, recommended for onward use in rice
breeding programs for possible introgression of the desired traits into cultivated traits.
PPBG 77

A TECHNIQUE FOR LAND MANAGEMENT ALTERNATIVES BASED ON

IDENTIFICATION OF APPROPRIATE TREE SPECIES CORRESPONDING TO
PARTICULAR SOIL ATTRIBUTES OF DISTRICT VEHARI, (PUNJAB), AND PAKISTAN
SYED MUHAMMAD AKMAL RAHIM1*, FARKHANDA JABEEN2 AND
SYED MUHAMMAD AJMAL RAHIM1
1

Punjab Forest Department, Lahore 54770, Pakistan

Department of Botany, University of the Punjab, Lahore 54770, Pakistan
*
Corresponding authors email: [email protected]

Overexploitation and environmental degradation has led to limited forestry resource base in Punjab, Pakistan. As the
wood production from the state forests fails to meet the ever increasing demand, currently, >90% of fuel wood and
nearly 50% of timber is being contributed by the private sector, through Agroforestry, that involves tree plantation on
agricultural farm lands. The main objective of the present study relates to the development of maps using GIS software,
enabling selection of tree species, keeping in view the ecological perspective. The first phase of the study involved the
identification of soil series; complete physical and chemical analysis of soil characteristics; and identification of any soil
attribute limiting tree growth. The second phase of the study was based on assessment of feasibility of a particular area in
District Vehari, Punjab Pakistan, for growing a particular tree species. The information generated from the first phase
was utilized to prepare overlay maps, on the basis of which most suitable species corresponding to particular soil
attribute could be identified. This technique offers time and cost effective land management options for agroforesters.
PPBG 78

IMPACT OF DIFFERENT PLANTING PATTERNS ON GROWTH, YIELD AND

RADIATION USE EFFICIENCY OF SPRING MAIZE (ZEA MAYS L)
IN SEMIARID ENVIRONMENT
TASNEEM KHALIQ, ASHFAQ AHMED, GHULAM MUSTAFA TAHIR,
JAMSHAD HUSSAIN AND M. IMRAN AKRAM
Agro-Climatology Lab., Department of Agronomy, University of Agriculture Faisalabad, Pakistan
*
Corresponding authors email: [email protected]
A field experiment was carried out in 2010 to determine the effect of planting patterns on growth, yield and
radiation use efficiency of spring maize (Zea mays L) hybrids. The experiment was laid out in Randomized Complete
Block Design (RCBD) with Split plot arrangement having four replications. Three Hybrids (Pak-HIB-7177, Pak-HIB7188 and FSH-793) were randomized in main plots and four planting patterns i.e. S1 (Single row (SR) flat sowing), S2
(Single row-twin row (SR-TR) flat sowing), S3 (SR ridge sowing) and S4 (Single side and double side (SR-DR) ridge
sowing) in sub plots. Results indicated that genotypes and planting patterns affected significantly leaf area index and
crop growth rate. Maximum leaf area duration on 87 DAS was 214 days in FSH-793. Overall mean leaf area duration
was 197 days in the season. The effect of planting patterns on the fraction of intercepted radiation was significant at all
stages of crop growth and was highest (0.9389) at 72 DAS captured by H1. Maximum plant height (155.9 cm), cob

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

49

length (18.27 cm), number of grains per cob (361), 1000-grain weight (237.6 g), grain yield (5868 kg ha-1) and biological
yield (16500 kg ha-1) was produced by FSH-793. Whereas S4 pattern gave the highest values of all these parameters. The
hybrid FSH-793 performed better compared to other hybrids and planting pattern S4 where maize was sown on ridges in
single row double row contributed higher grain yield compared to other planting patterns.
PPBG 79

EVALUATION OF MOLECULAR MAPPING POPULATION FROM

WHEAT/SYNTHETIC HEXAPLOID CROSSES FOR DROUGHT TOLERANCE
WAQAS HUSSAIN1, ZAHID AKRAM1*, TALAT MEHMOOD1, GHULAM SHABBIR1,
REHMATULLAH QURESHI1 AND ABDUL MUJEEB KAZI2
1

Department of Plant Breeding and Genetics, PMAS-Arid Agriculture University Rawalpindi

2
Wheat Wide Crosses Program, NARC, Islamabad
*
Corresponding authors email: [email protected]

The novel genetic diversity of D genome can be exploited to address the devastating problem of drought in wheat
crop. To enhance the breeding efficiency in stress prone environments globally several molecular mapping populations
have been produced. The focus of this study was to phenotype the drought molecular mapping population (DR.MP-3),
derived from an opata/synthetic wheat hexaploid cross combination by various morphological and physiological
parameters for drought tolerance under In vivo and In vitro conditions.The results from the morphological parameters
showed that three genotypes viz. 118, 23 and 14 performed better for spike length, number of grains per spike and 1000
grain weight equally, under drought stress conditions. These genotypes also showed better defensive mechanisms under
In vitro conditions against drought through high accumulation of proline and more antioxidant activity. The best lines of
drought mapping population based on both morphological and physiological evaluation were 14, 17, 23, 55, 108, 118,
122, 150 and 152, as these genotypes performed best equally under both In vivo and In vitro testing. The present study
based on morphological and physiological evaluation suggested that unique genetic diversity from Aegilops tauschii can
be harnessed to get more yield by improving the existing cultivars against abiotic stress.
PPBG 80

SALICYLIC ACID PRE-SOAKING FOR GERMINATION OF SWEET PEPPER

UNDER SALT STRESS
WAZIR AHMED1*, MUHAMMAD YASEEN1, MUHAMMAD ARSHAD1 AND MUHAMMAD SHAHID2
1

Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad

Department of Department of Chemistry and Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad
*
Correspondence authors e-mail: [email protected]

A laboratory study was conducted to examine the role of salicylic acid (SA) pre-soaking on Capsicum annum seed
germination and seedling vigor under salinity stress. The study was carried out in Sanyo Incubator MIR-253 at 24oC. A
set of seeds was pre-soaked with H2O whereas three set of seeds were pre-soaked with 0.1, 0.3 and 0.5 mM salicylic acid
(SA), respectively for 3 hours under aerobic conditions. Three levels of salinity (0, 5 and 10 dS m-1) were used. Four
seeds were placed on whatman No. 42 in each petridish. Data related to germination of seeds was recorded after an
interval of 24 h. It was observed that SA pre-soaked seeds show an increase in percent germination rate (40-60%),
chlorophyll contents measured with portable chlorophyll meter (30-70%), seedling biomass (10-30%), shoot length (826%) and root length (12-40%) compared to H2O pre-soaked seeds under salinity stress. Results suggest that pre-soaking
of sweet pepper seeds with 0.3 mm SA has potential to mitigate reduction in seed germination of sweet pepper.
PPBG 81

EVALUATION OF RICE HULL AS POTTING SUBSTRATE FOR GROWTH AND

FLOWERING OF DAHLIA HORTENSIS
USMAN TARIQ1*, ADNAN YOUNIS1, SHOAIB UR REHMAN1, ATIF RIAZ1 AND MANSOOR HAMEED2
1

Institute of Horticultural Sciences, University of Agriculture, Faisalabad 38040

2
Department of Botany, University of Agriculture, Faisalabad 38040
*
Corresponding authors email: [email protected]

The effects of rice hull as potting substrates on growth and flowering of Dahlia hortensis was studied. Plants were grown
in rice hull substrate in different combinations with silt. The experiment was carried out in completely randomized design
(CRD) with five treatments. The results associated with plant growth indicated that plant height, number of leaves, number of

AGRICULTURE AGRONOMY, HORTICULTURE, PLANT BREEDING & GENETICS

50

flowers and corm weight were the maximum in silt+ rice hull combination (1:1 ratio by volume). Dahlia plants grown in silt+
rice hull combination (1:2 ratio by volume) produced early flowering, maximum flower diameter, flower stalk and side shoots.
Data regarding number of corms per plant was statistically non-significant. The present study revealed that a rice mill waste in
the form of rice hull is a good and cheap source as potting substrate constituent for the production and flowering of dahlia
plants.
PPBG 82

EVALUATION OF VARIOUS HERBICIDES FOR WEED MANAGEMENT IN

POTATO IN PESHAWAR PAKISTAN
ZAHID HUSSAIN1*, FAZAL MUNSIF2, KHAN BAHADAR MARWAT1,
RIAZ AHMAD AFRIDI3 AND SHAHIDA BIBI1
1

Department of Weed Science, 2Department of Agronomy, KP Agricultural University Peshawar

3
Agriculture Research Institute Tarnab Peshawar
*
Corresponding authors email: [email protected]

Weed control is one of the most important factors for a successful crop production and therefore the prevention of
weedcrop competition at an early stage plays a very important role. Potato is grown at large scale in Khyber
Pakhtunkhwa-Pakistan, but due to lack of proper attention potato yield is always quite lower in comparison with the
developed countries. Besides, other factors, weeds are one of the serious obstacles in producing lower yields. In this
context, an experiment was designed during 2009-10 at Agriculture Research Institute Tarnab Peshawar Khuber
Pakhtunkhwa Pakistan, in which there were a total of nine treatments including eight herbicides and a weedy check.
Metribuzin 70% WP (metribuzin), Gramoxone 200 SL + Dual gold 960EC (gramoxone + s-metolachlor), Sencor 70 WP
(metribuzin), Torrent 50 WDG (terbutryn + terbuthylazine), Solanum 500 SC (terbutryn + terbuthylazine), Dual gold
960EC (s-metolachlor), Multiquat 200 SL (gramoxone) and Gramoxone 200 SL (gramoxone) were the herbicidal
treatments that were evaluated. Among the herbicides, Metribuzin 70% WP, Torrent 50 WDG, Solanum 500 SC, and
Multiquat 200 SL were used as candidate herbicides for registration purpose and the rest of the herbicides were standard
ones for comparative evaluation. The results obtained revealed that all the herbicides had a significant effect on weed
density m-2 and also on tuber yield of potato. Herbicidal treatments convincingly reduced the weed population as
compared to the control treatments having 104 weeds m-2. The reduction in weed population ranged between 80.5 to
95.7% in the herbicidal treatments which was quite impressive effect. No crop injury was observed in any of the
herbicides used in the experiment; however the crop was kept in shelter while using the contact herbicides, gramoxone.
The herbicide treatments of Gramoxone 200 SL + Dual gold 960 EC @ 3.75 lit + 2.50 lit, Gramoxone 200 SL @ 3.75 lit,
and Multiquat 200 SL @ 3.75 lit ha-1 gave best potato tuber yields i.e. 15910 kg, 15288 kg, and 15022 kg ha-1,
respectively, which were statistically similar as well. Therefore, the respective increase in yields over weedy check was
36.13, 33.53, and 32.35% over weedy check treatments (10162 kg ha-1). It can be concluded that gramoxone can result in
best yields and must be used for weed control in potato using a shelter for the crop to avoid any injury.

BIOTECHNOLGY, BIOCHEMISTRY AND BIOINFORMATICS

51

ORAL ABSTRACTS
OPBB 1

COMPARATIVE ASSESSMENT OF GLUTENIN COMPOSITION AND THEIR

RELATIONSHIP WITH GRAIN QUALITY TRAITS IN BREAD WHEAT GERMPLASM
AHMAD ALI1*, MUHAMMAD ARSHAD1, ANNA MARIA MASTRANGELO2, PASQUALE DE VITA2,
AWAIS RASHEED3, ALVINA GUL-KAZI4, TARIQ MAHMOOD3 AND ABDUL MUJEEB-KAZI5
1

Department of Botany, Pir Mehr Ali Shah, Arid Agriculture University, Rawalpind
2
CRA-Cereal Research Centre, Strada Statale16, km 675, Foggia, Italy
3
Plant Biochemistry and Molecular Biology Lab, Department of Plant Sciences, Qauid-i-Azam University, Islamabad
4
National University of Science and Technology (NUST) Islamabad
5
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad.
*
Corresponding authors email: [email protected]
Baking industry exigencies and wider consumer preferences are highly demanding variable end product use of bread
wheat. The diversity in bread wheat to fulfill these demands is primarily dependent on the D-genome encoded glutenins
complemented by desirable glutenins from the A- and B- genome. The present study was designed to evaluate and
compare glutenin compositions and their effect on key quality parameters in D-genome synthetic hexaploid derivatives
(SHD) and conventional bread wheat (BW) germplasm. The germplasm set selected encompasses the earlier investigated
drought tolerant characteristics. Grain quality analyses have provided a stringent selection sieve to select the drought
tolerant genotypes with desirable end quality characteristics. Several unique D-genome encoded HMW-GS were found
along with favorable alleles at A- and B-genomes. D-genome encoded subunit Dx5+Dy10 which is known to encode
superior grain quality attributes was observed in 63.64% genotypes followed by 1Dx2+1Dy12 (30.91%). Apart from
HMW-GS, PCR based allele specific markers were used to identify allelic variation at Glu-3 loci (LMW-GS), which had
a significant effect on visco-elastic properties of wheat dough. These analyses are more robust and now considered
superior over the earlier conventional technique of SDS-PAGE for identifying LMW-GS. Several combinations of
favorable LMW-GS alleles were observed at Glu-A3 and Glu-B3 loci. Key quality parameters like protein,
sedimentation volume and carotenoids differed significantly within genotypes. Higher values for desirable quality values
were found in synthetic derivative genotypes and as well as in conventional bread wheat varieties. Our results have
established significant variability in quality characteristics and glutenin composition among D-genome synthetic
hexaploid wheat derivatives as compared to conventional bread wheat germplasm suggestive of their ability to improve
quality traits in bread wheat.
OPBB 2

QUANTITATIVE TRAIT LOCI MAPPING OF DROUGHT TOLERANCE AT

GERMINAITON STAGE IN BREAD WHEAT
ARMGHAN SHAHZAD1, 2*, SAMI ASHRAF2, FARWA KARAMAT2, MUHAMMAD IQBAL1,2, JALAL-UDDIN2,3 AND GHULAM MUHAMMAD ALI1,2
1
2

Plant Biotechnology Program (NIGAB), National Agriculture Research Center, Park Road 45500 Islamabad.
Department of Plant Genomics and Biotechnology, PARC Institute of Advance Studies in Agriculture, National
Agricultural Research Center, Park Road 45500 Islamabad.
3
Stress Physiology Program, National Agricultural Research Center, Park Road 45500 Islamabad.
*
Corresponding authors email: [email protected],

Drought is a major yield limiting factor in many crop species. Wheat is a major crop and staple food in Pakistan.
Genetic linkage map construction based on linked DNA markers spanning whole wheat genome and subsequent QTL
mapping for drought tolerance can enhance breeders ability for effective selection. We used an F8 population (80 lines)
derived from the cross of OPATA x SH-349 for drought tolerance at germination stage under controlled conditions. The
drought was induced by 15% PEG nutrient solution in acid washed sand medium under controlled conditions (14/10 hrs
light and dark and 250C). During four weeks of growth, data for germination percentage, germination rate index, shoot
root lengths and dry weights were recorded. These data along with marker data were used for QTL mapping using QTL
Cartographer V2.5 software. Single marker QTL analysis showed that 14 SSR markers were linked to QTLs for five
traits in both drought and control condition. Using simple interval mapping and composite interval mapping, QTLs for
different traits of interest were mapped on two linkage groups. On linkage group 1, QTLs for root length, shoot length,
dry biomass and germination percentage were mapped under control and drought conditions. On linkage group 2, QTLs
for germination percentage and germination rate were mapped under controlled and drought conditions. The population
will be screened with more SSR markers to increase the genomic coverage of this map.

52

BIOTECHNOLGY, BIOCHEMISTRY AND BIOINFORMATICS

OPBB 3

COMPARATIVE ARTEMISININ ANALYSIS IN ARTEMISIA ANNUA AND ARTEMISIA

DUBIA TRANSFORMED WITH TWO DIFFERENT AGROBACTERIA
HARBOURING ROL ABC GENES
BUSHRA HAFEEZ KIANI AND BUSHRA MIRZA
Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan
Artemisinin, a potent antimalarial drug and a major constituent of Artemisia, is effective against quinine resistant
strains of Plasmodium. Chemically it is an endoperoxide sesquiterpene lactone. It is a potent antimalarial drug that has
also been proven very effective in treatment of cancer. The rol ABC genes have been shown to enhance production of
secondary metabolites in plants, possibly through stimulation of defense pathway. The aim of the present study was to
enhance artemisinin production in the transformed and untransformed plants of Artemisia annua and Artemisia dubia
through transformation with rol genes by using Agrobacterium tumefaciens and Agrobacterium rhizogenes.
Agrobacterium tumefaciens strain LBA4404 containing pRT99 and Agrobacterium rhizogenes strain LBA8196 and 9402
harboring rol ABC genes were used for the transformation experiments. PCR and Southern hybridization confirmed the
T-DNA integration events in Artemisia dubia roots. The transformed roots proliferated much more vigorously compared
to untransformed roots. HPLC analysis showed higher concentration of artemisinin in transformed plants of both
A.annua and A.dubia transformed with control plants while no artemisinin was observed in control roots. Transcriptomic
study of A.annua and A.dubia allowed sequencing the transcriptome of these species for the first time. 16400 Contigs
were generated by aligning different transcriptome sequences. BLAST of 500 contigs was performed with nt/nr database.
264 contigs showed homology with genomes of different organisms. Up and down regulation and Putative gene
functions were predicted.
OPBB 4

DYNAMICS OF mRNA OF GLYCINE-RICH RNA-BINDING PROTEIN DURING WOUNDING,

COLD AND SALT STRESSES IN NICOTIANA TABACUM
FARIHA KHAN, TASAWAR SULTANA, FARAH DEEBA AND S.M. SAQLAN NAQVI
Department of Biochemistry, PMAS Arid Agriculture University Rawalpindi, Pakistan
*
Corresponding authors email: [email protected]
Glycine-rich RNA-binding proteins (GRPs) are RNA-binding proteins that contain one or more RNA recognition motif
(RRM) or consensus RNA-binding domains at their N-terminus and a glycine-rich domain at their C-terminus. Their
function is not well characterized but GRPs are implicated in plant stress responses, owing to the fact that their mRNA level
increases under these conditions. In order to gain an insight into the role at molecular level, the study was designed to
observe the dynamics of NtGRP1 during exposure to stresses i.e. wounding, cold and salt stress by real-time PCR.
Expression was found to be modulated by wounding in five months old plants. For cold stress, plants were transferred to
4C for 24 hours at different developmental stages, wherein expression of mRNA was found to be up-regulated. Variation in
transcript level was also seen in response to treatment with various concentrations of NaCl. The detailed characterization of
GRPs is expected to enhance our understanding about role of GRPs in plant stress metabolism.
OPBB 5

MOLECULAR CLONING AND CHARACTERIZATION OF ARF1 AND COPI COAT

PROTEINS FROM MEDICAGO TRUNCATULA CV. JEMALONG
HOLGER DENSOW1, ABDUL RAZAQUE MEMON2, ELIF YUZBASIOGLU3,MATTHIAS FRICKE4,
MARKUS SAUER4 AND KARSTEN NIEHAUS1
1

Department of Proteome and Metabolome Research, Bielefeld, University, POB 100131, D-33501 Bielefeld, Germany
2
Department of Genetics and Bioengineering, International Burch University, Francuske revolucije bb, 71000,
Sarajevo, Bosnia and Herzegovina
3
Istanbul University, Faculty of Science, Department of Biology, Istanbul, Turkey
4
Bielefeld University, Department of Physics, POB 100131, D-33501 Bielefeld, Germany

The integrity of the Golgi apparatus in both plant and mammalian cells is dependent upon a coordinated flow of
COPII (coatomer protein) coated vesicles in anterograde (forward) and of COPI coated vesicles in retrograde (backward)

BIOTECHNOLGY, BIOCHEMISTRY AND BIOINFORMATICS

53

direction. Although a fair amount of work on vesicular trafficking has been published in Arabidopsis thaliana not much
information is available related to the secretory pathway in other higher plants. In the present study we have used
Medicago truncatula, a model plant for legume species as for symbiotic and pathogenic interactions, to identify Arf1 and
COPI components of the early secretory pathway. Their localisation and interaction with the Golgi apparatus in the root
cells has been identified by confocal and two-photon laser microscopy. EST databases of the M. truncatula were
screened and putative homologues for all seven coatomer proteins and MtArf1 were identified. Two isoforms of the zCOPI subunit and MtArf1 genes were isolated from M. truncatula cDNA libraries and were sequenced. GFP fusions of
MtArf1, dominant active (Q71L), dominant inactive (T31N) forms of MtArf1and two isoforms of MtzCOP1 were
expressed in transgenic hairy root and their subcellular localisation were analyzed. Our results show that
MtArf111::GFP, Mtz-COP1::GFP and Mtz COP2::GFP were localized on mobile Golgi structures, streaming along the
ER network and were sensitive to brefeldin A, indicating their potential association with Golgi stacks. This study
demonstrates an important role of Arf1 and COP1 proteins in early secretory pathway in root cells of M. truncatula.
OPBB 6

BACATERIAL BIOFILM FORMATION INHIBITION BY BLOCKING

FLAGELLAR ASSEMBLY
IRAM LIAQAT1 AND SHAHID KHAN2
1,2

Institute of Microbiology, University of Agriculture, Faisalabad

Department of Biology, LUMS-school of Science and Engineering, Lahore

Biofilms formation is a major hazardous problem from both clinical and environmental perspective. Flagellummediated motility is important for biofilm formation by several gram-negative bacteria. >50 genes are involved in
flagellar biosynthesis and function in Salmonella typhimurium. The flagella basal body is a representative of Type III
protein secretion systems; used by several gram-negative bacterial pathogens to colonize foreign tissues and substrates.
The mechanism of flagellar assembly was analyzed in S. typhimurium, using bioinformatics analysis to identify
conserved structural elements. In this study, FliI a flagellar protein that is needed for flagellar assembly and may be
involved in a specialized protein export pathway was cloned and overexpressed. Using vital dyes, visualization of single
and motile was established based on optical microscopy techniques which will extend initial evidence that flagellummediated rotation is critical for biofilm formation. The flagellar basal body is a particularly convenient drug target, since
the architecture of most its components has been determined near atomic resolution and it is an ancient evolutionarily
conserved macromolecular assembly. The knowledge gained will also have implications for elucidation of the
mechanistic design principles underlying protein secretion complexes.
OPBB 7

IN VITRO CYTOTOXIC ENHANCING ACTIVITY OF TRITERPEN SAPONINS FROM

GYPSOPHILA PILULIFERA ON SAPORIN A TYPE-I RIPS
IDRIS ARSLAN1, ALI CELIK1, KRISTINA JENETT-SIEMS2 AND MATTHIAS F. MELZIG2
1

Pamukkale University, Faculty of Science and Arts, Biology, TR20017, Denizli, Turkey
Freie Universitt Berlin, Institute of Pharmacy, Pharmaceutical Biology, Knigin Luise str.2+4 D14195, Berlin, Deutschland

A cytotoxic triterpenoid saponin was isolated from the under-ground parts of Gypsophila pilulifera Boiss.& Heldr.
(Caryophyllaceae) naturally grow in the southwestern region of the Turkey. The structures of saponin was elucidated as
3-O--D-galactopyranosyl-(12)-[-D-xylopyranosyl-(13)]--D-glucuronopyranosyl quillaic acid 28-O--Dglucopyranosyl-(13)-[-D-xylopyranosyl-(14]--L-rhamnopyranosyl-(12)--D-fucopyranosyl ester on the basis
of extensive spectral analysis and chemical evidence. The separated triterpenoid saponin was isolated from Gypsophila
pilulifera for the first time. The saponin compound displayed significant cytotoxicity against A549 cell line with IC50
values >16M.
OPBB 8

TISSUE CULTURE INDUCED SOMACLONAL VARIATION:

A POTENTIAL SOURCE OF GENETIC VARIABILITY FOR DEVELOPING
DROUGHT TOLERANT PLANTS OF WHEAT
IMRAN MAHMOOD1, ABDUL RAZZAQ1*, MUHAMMAD RASHEED1, ABDUL QAYYUM1, MUHAMMAD
AHMAD1, M. M. QADEER BAIG2

BIOTECHNOLGY, BIOCHEMISTRY AND BIOINFORMATICS

54

Department of Agronomy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
2Department of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
*Correspondence: [email protected]
Drought, a serious threat to world agriculture, demands neo-breeding approaches. Tissue culture being a mutagenic
process induces somaclonal variations, which can be manipulated for improving drought tolerance of commercial
cultivars of wheat. Present study was conducted to explore potential of somaclonal variation to improve drought
tolerance of wheat. Micro-clumps of calli induced from immature embryos of wheat cv. GA-2002 were cultured on MS
based medium supplemented with PEG-6000 induced osmotic stress of -0.9 MPa for four week followed by another
selection cycle. PEG-6000 tolerant calli were regenerated to R0 somaclones and selfed to produce R1 seeds. The
progeny of R1 seeds (R1 generation) and their donor parent cv. GA-2002 were raised in pots and compared for drought
tolerance. Artificial water stress was induced by withholding water for 2, 4, 6, and 8 days along with control. Water
stress led to a reduction of relative water content (RWC), excise leaf water loss (ELWL), leaf succulence and specific
leaf weight (SLW), while an increase in ABA content of both R1 somaclones and their parent cv. GA-2002. The R1
somaclones showed significantly greater tendency to conserve RWC, leaf succulence and less ELWL in response to
higher regimes of water stress imposed for six or eight days. Similarly, significantly higher ABA contents were
accumulated by R1 somaclones than parent cv. GA-2002 in response to water stress of 4, 6 and 8 days. Results from
physiological bases of drought tolerance indicated that somaclonal mutants had higher drought tolerance than their
parent cv. GA-2002. It is concluded that tissue culture induced somaclonal variation are potential source of genetic
variability to be exploited for developing drought tolerant plants of wheat in the presence of suitable selection agent.
OPBB 9

AMPLIFICATION AND SEQUENCING OF INTERNAL TRANSCRIBED REGIONS 1& 2,

AND 5.8S RDNA FROM LOCAL ISOLATES OF FUSARIUM SPECIES
KANWAL BATOOL1, M. SHEERAZ AHMAD 1, CHAUDHARY ABDUL RAUF2 AND S.M. SAQLAN NAQVI1*
1

Department of Biochemistry
Department of Plant Pathology, PMAS Arid Agriculture University Rawalpindi
*
Corresponding authors email: [email protected]

Fusarium oxysporum is a phytopathogenic fungus. It is widely distributed around the globe. Classical classification
of F. oxysporum is based on phenotypic observations which highly vary and are also sensitive to environment. In
Fusarium taxonomy this problem is recently being addressed by sequence comparison at different loci. Internally
transcribed spacer (ITS) region including 5.8S rRNA coding region in ribosomal DNA is one of the favorite targets for
this purpose. The focus of present study was on the genetic diversity analysis of the ITS regions of rRNA gene complex
of local isolates of Fusarium. The genomic DNA of these isolates was amplified using FoxF, FoxR and FoxIR primers
designed at the end, and start of conserved 18S region, and between ITS1 and ITS2 respectively. FoxF and FoxR primer
set amplified ~500 bp product from all F. oxysporum strains. The amplified products were sequenced and sequence
analyses have shown that F. oxysporum f.sp. ciceri strains couple of SNPs. Similarly F. oxysporum f. sp. lentis has
shown variations with with two strains of F. oxysporum f. sp. ciceri at two position. Comparison of F. moniliforme
isolates with F. oxysporum isolates have revealed that 5.8S region is identical in all isolates while significant sequence
variation was observed in ITS regions of F. oxysporum and F. moniliforme. Insertions and deletions of many
nucleotides were observed at several positions which differentiate F. moniliforme from F. oxysporum. The phylogenetic
analysis revealed no significant different among local isolates and internationally reported sequences. From a clear
grouping of F. moniliforme and F. oxysporum isolates into different clades it may be evident that ITS regions are useful
for classifying F. oxysporum isolates at specie level.
OPBB 10

TRANSGENIC TOBACCO WITH RICE FAE GENE SHOWS ENHANCED

RESISTANCE TO DROUGHT STRESS
KHIZAR HAYAT BHATTI1*, AMINULLAH SHAH2, QAISAR MEHMOOD3, KHALID NAWAZ1, KHALID
HUSSAIN1, EJAZ HUSSAIN SIDDIQI1, WU JIAHE4 AND ZHU CHUANFENG4
1

Department of Botany, Institute of Chemical and Biological Sciences, University of Gujrat, Gujrat-50700, Pakistan
2
Department of Biological Sciences, University of Sargodha, Sargodha-40100, Pakistan
3
Department of Environmental Sciences, COMSATS-IIT, Abbottabad-22060, Pakistan

BIOTECHNOLGY, BIOCHEMISTRY AND BIOINFORMATICS

55

Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing-100101, China.

*
Corresponding authors e-mail: [email protected]

Plants have evolved various adaptative traits to cope successfully with the stresses. Among them, cuticular waxy
coating layer may serve as protecting barrier to diminish water loss, which consequently imparts drought resistance in
plants. In order to characterize the role of rice FAE in drought tolerance, the OsFAE transgene was incorporated into
tobacco via Agrobacteriummediated leaflets transformation with sense sequence orientation under control of
constitutive promoter CaMV35S. PCR and RT-PCR assays suggest that the OsFAE transgene has incorporated in
tobacco genome and over-expressed in the transformed tobacco leaves. The characterization assay revealed some
correlation between OsFAE transgene expression and drought tolerance in transgenic tobacco. The drought parameters
data reveal that the transformed tobacco lines exhibit relatively less wilting on withheld-water stress, early recovery from
the stress, containing higher relative water contents. Additionally, the transgenic tobacco lines exhibit more protein
contents after exposure to sub-lethal drought stress and relatively higher contents were measured in them as compared to
control on re-watering after 48 hours. Proline contents were found higher in the transgenic lines as compared to control
under drought on 6th day of with-held water stress. Data shows that leaf water potential was less negative in the selected
transgenic lines as compared to control on both; 10th day of with-held water stress and after 24 hours rehydration.
OPBB 11

MINING OF POLYMORPHIC MICROSATELLITE MARKERS IN SILICO FOR

DIAGNOSIS OF BASMATI RICE ADULTERATION
MARIAM RUKHAMA FARMAN, MARIA SALEEM, SAADIA NASEEM, FAUZIA YUSUF HAFEEZ
Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Park Road, Islamabad, Pakistan
Microsatellite markers including Simple Sequence Repeats (SSRs) are used to differentiate between species
including closely related cultivars due to their uniqueness for genome. The objective of the present study is to device a
molecular maker based procedure for the detection of adulteration of the highly valued basmati rice with non-basmati
rice. 10 publicly available genomic sequences for basmati rice (Oryza sativa indica group) were retrieved from the NCBI
database. The manipulation of these sequences was carried out for mining microsatellites, a total of 3258 microsatellites
were identified. This was accomplished by the use of microsatellite development software SSR Locator. The same
software designed 99 primers specific to the identified SSR markers. Simulated polymerase chain reaction (PCR)
eliminated primer redundancy to select 40 potential primers generating specific amplicons. The selected primer pairs
were analyzed further for their specificity by using NCBI BLASTN tool which ascertained that 4 out of the 99 designed
primers are specific solely to the basmati rice (indica group) genome, hence assuring the further utility of these primers
for the differentiations of the basmati rice from non-basmati rice for the eventual detection of adulteration in these two
cultivars of rice. Similarly, three complete sequences of non-basmati (japonica) rice were retrieved and from 4788
primers that were generated for japonica we propose 2 primer pairs specific to japonica rice cultivar to use for the
verification on adulteration of no-basmati rice in basmati rice sorts.
OPBB 12

DEVELOPMENT OF PUTATIVE MOLECULAR MARKERS TO TRACE DURABLE

RUST RESISTANCE GENES IN WHEAT BREEDING STOCKS
MARYUM ZEB1, MUHAMMAD KAUSAR NAWAZ2 AND RANI FARYAL1
1

Dept. of Biosciences, COMSATS Institute of Information Technology, Chak Shahzad, Park Road, Islamabad, Pakistan
2
Dept. of Plant Breeding and Genetics, PMAS Arid Agriculture University, Rawalpindi, Pakistan

Wheat plays a central role in Pakistans food economy in terms of production and consumption. Wheat shares 3.1%
of national GDP. Rusts are among the devastating diseases of wheat. A large number of rust resistance genes are known
in wheat for stripe (yellow), leaf (brown) and stem rust. Selection for durable resistance in rust breeding programs is
typically carried out in field screening nurseries or under controlled conditions only for advanced breeding lines. Marker
Assisted Selection (MAS) can be useful under such conditions. Availability of small number of DNA markers for
identification of genes involved in rust resistance makes MAS application difficult. Identification and designing of new
PCR primers may help to select rust resistant genotypes from wheat breeding stocks in early segregating generations.
This study is designed with aim to identify and design PCR primers for resistance genes in wheat breeding stocks. Data
and sequences were retrieved through extensive literature and databases search. Data mining was done on public domain
available databases i.e., NCBI nucleotide, GrainGenes and PlantGDB. A total of 12 sequences for leaf rust resistance, 13
for stripe rust resistance and 2 for stem rust resistance were retrieved. Homology studies, motif finding and their
phylogenetic analysis was done to infer their possible functions and to develop PCR primers to mark the rust resistance
genes in wheat germplasm. These markers were validated for detection of rust resistance gene among 16 local varieties,
which proved valuable for future wheat breeding.

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OPBB 13

MOLECULAR MAPPING OF STRIPE RUST RESISTANCE GENE

YRSN78 IN WHEAT LINE SHAANNONG78
MUHAMMAD AZEEM ASAD1,4*, YAN REN1, XIANCHUN XIA1, CHENGSHE WANG2 AND ZHONGHU HE1,3
1

Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and
Genetic Improvement, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, China
2
College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China
3
International Maize and Wheat Improvement Center, China Office, Beijing 100081, China
4
Nuclear Institute of Agriculture, Tando Jam, 70060, Pakistan
*
Corresponding authors email: [email protected]

Stripe rust, caused by Puccinia striiformis f. sp. tritici is an important disease in wheat growing areas worldwide.
Growing resistant cultivar is the best strategy to control stripe rust. The wheat line Shaannong 78 displays race-specific
resistance to stripe rust. To identify the genetic basis of resistance, Shaannong 78 was crossed with susceptible cultivar
Mingxian 169. Seedlings of the parents and F1, F2, and F3 populations were inoculated with race CYR 32 of Puccinia
striiformis f. sp. tritici under controlled green house conditions. One single dominant gene conferring resistance to stripe
rust was identified, designated as YrSN78. The resistance gene YrSN78 was tagged by screening 1,528 simple sequence
repeat (SSR), and two 1BL.1RS linked markers (XAF4 and XH20). In bulked segregant analysis, three molecular
markers XAF4, XH20, and Xbarc8 were closely linked to YrSN78 and mapped to chromosome 1BL.1RS with genetic
distances 1.7 and 2.8cM. Pedigree analysis and linked molecular markers suggested that YrSN78 is different from
previously identified genes Yr9, YrCn17 and YrR212, and it may be new gene or allele at Yr9 on chromosome
1BL.1RS. The gene YrSN78 in combination with other stripe rust resistance genes, and linked molecular markers could
be useful in marker assisted breeding programs for stripe rust resistance in wheat
OPBB 14

OPTIMIZATION OF QUANTITATIVE REAL-TIME PCR ANALYSIS FOR RELIABLE

DETECTION AND QUANTIFICATION OF FUSARIUM OXYSPORUM IN WHEAT
M. FAROOQ HUSSAIN MUNIS, ASGHARI BANO, HASSAN JAVED CHAUDHARY, MUHAMMADI
Department of Plant Sciences, Quaid-i-Azam University, Islamabad
Early detection of Fusarium oxysporum in wheat is very important for its effective control. Use of quantitative realtime PCR is a modern technique to detect and quantify fungal intensity. In this experiment, this technique has been used
to see susceptibility of four wheat cultivars (CS-12, FD-05, TJ-9, and ZY-19) to Fusarium oxysporum. Typical Fusarium
oxysporum symptoms like slight vein clearing, epinasty and wilting of leaves developed 8 days post infection (dpi) and
became severe 15 dpi. Apparently, these symptoms were more prominent in FD-05 and TJ-9, as compared to other two
varieties. Quantification of Fusarium oxysporum DNA in wheat seedlings on 8 and 15 dpi revealed the highest amount
of DNA in FD-05 and relatively higher amount of DNA in TJ-9 and CS-12 while the lowest DNA concentration was
found in ZY-19. The amount of fungal DNA in FD-05 remained constant on 8 and 15 dpi while it increased in all other
lines. In addition, real-time PCR was used as Fusarium oxysporum detection method in wheat field plants, irrigation
water and soil and the fungus was successfully detected in these field samples before the appearance of typical
symptoms. These results provided us an easy and comprehensive approach for reliable detection of Fusarium oxysporum
at early infection stages.
OPBB 15

LEAF PROTEOME ANALYSIS OF CLEMATIS CHINENSIS: A TRADITIONAL CHINESE

MEDICINE (TCM) BY TWO-DIMENSIONAL ELECTROPHORESIS TECHNIQUE
MUHAMMAD ISHTIAQ CH1*, ABDUL SAMAD MUMTAZ2, MEHWISH MAQBOOL1 AND YI WANG3
1

Department of Botany, Mirpur University of Science & Technology, Bhimber (Azad Kashmir), Pakistan
2
Department of Plant Sciences, Quaid-e-Azam University, Islamabad, Pakistan
3
Institute of Bioinformatics, Department of Chinese Traditional Medicines, College of Pharmaceutical Sciences,
Zhejiang, University, Hangzhou, P. R. China.
*
Corresponding authors email: [email protected]
Two-dimensional electrophoresis (2-DE) technique has become an important integral part of taxonomic, phylogenetic and
pharmaceutic studies in plants. Proteomics has been used here in identification of different taxa of genus Clematis employed as

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57

Traditional Chinese Medicines (TCMs) in China. Leaf proteins of Clematis chinesis and some other species of Clematis were
extracted using three different methods and their profiles resolved on 2-DE. Comparative analysis of employed extraction
methods revealed phenol-SDS method (PSM) to be the best protocol having protein quantity i.e. 2.350.345 mg/g. For
analytical and preparative runs proteins were detected by silver staining and comassie blue (CBB) staining, respectively.
Master maps of the three replicas of each species were compared by ImageMaster 2D Platinum software (Amersham
Biosciences). The results of this differential analysis predicted that nine proteins were solely present in C. chinensis when
compared with C. finetiana and C. armandii.The 2-DE gel pictorial profile depicted general distribution of 1085 spots on
stained gels and out of these only 255 protein spots (23.5%) were common to all analyzed taxa. The visualized protein spots
showed pI range from 3.0 to 10.0 (pH) and Mr of 7 kDa to 70 kDa. Nine proteins were exclusively specific to C. chinensis
when compared with C. finetiana and C. armandii. A trend of up-regulation in thirteen proteins in C. finetiana (0.75-0.95 fold)
and twelve proteins in C. armandii (1.05-1.66 fold) was found. A reverse perspective of down-regulation for seven proteins
(0.66-0.94 fold) was seen in C. finetiana and for three proteins (1.07-1.20 fold) in C. armandii. Intra-section genetic distance
(GD) was estimated 0.4~0.45 in analyzed species of subsection Clematis and inter-section GD was 0.25~0.55 among species
of subsection Rectae. Proteins were identified by LC-MS/MS technique. Identified proteins are mostly related to energy
metabolism (ATP synthesis), photosynthesis, environmental stimuli, regulating RNA metabolism, regulating growth hormone,
mitochondrial functions and regulating gene expression in response to cellular state. The efficiency and applicability of
proteomic approach as biomarker for identification of TCMs and other taxa has been described discussed.
OPBB 16

PROFILING THE CARROT (DAUCUS CAROTA) MICRORNAS AND THEIR TARGETS

MUHAMMAD YOUNAS KHAN BAROZAI
Department of Botany, University of Balochistan, Sariab Road Quetta, Pakistan
*
Corresponding authors email :[email protected]
MicroRNAs (miRNAs) are small, non-protein coding and negative regulatory RNAs approximately 18-26
nucleotides in length. The comparative genomic methodology due to their conserved nature is a rational approach for the
novel miRNAs discovery. In this study, total 17 novel miRNAs from 12 families were identified in an important
vegetable carrot (Daucus carota). All the miRNA families (dca-mir-156, 160, 167, 172, 774, 778, 854, 1310, 5015, 5030,
5658 and 5664) are found for the first time in carrot. All 17 miRNA precursors form stable minimum free energy
secondary structures and the mature miRNAs reside in the stem region of the secondary structures. Their putative targets
were also identified. These findings will be useful to understand the complicated negative gene regulation in an
important plant carrot.
OPBB 17

HPLC-DAD ANALYSIS AND FREE RADICAL SCAVENGING POTENTIAL

OF QUERCUS DILATATA L
MUNAZZA IHTISHAM1, IHSAN-UL-HAQ2, SARA SARWAR1, SAMREEN SALEEM1,
LAILA JAFRI1, NAZIF ULLAH1 AND BUSHRA MIRZA1*
1

Department of Biochemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan.

2
Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, Pakistan.
*
Corresponding authors email: [email protected]

Free radicals are molecules or atoms that have at least one unpaired electron which increases the chemical reactivity
of the molecule. The main objective of the present study was to evaluate the antioxidant potential and HPLC analysis of
antioxidant compounds (rutin, quercetin and gallic acid) in Quercus dilatata L. The antioxidant activity of Q. dilatata L.
extracts/fractions was determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay.
Four partitioned fractions of Q. dilatata (n-hexane, ethyl acetate, n-butanol and aqueous) were prepared among which
ethyl acetate fraction showed highest activity (IC50 38.02 g/ml). Furthermore, the screening of rutin, quercetin and
gallic acid in the partitioned fractions was done by HPLC-DAD which showed that the most active fraction i.e. ethyl
acetate fraction contained all of them while aqueous fraction showed the presence of two i.e., rutin and gallic acid.
Butanol fraction showed only rutin content, while n-hexane fraction did not show the presence of any of the above
mentioned compounds. Thus it can be concluded that good antioxidant potentials may be due to the presence of these
well known antioxidant compounds in Q. dilatata in association with other unidentified compounds.

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58

OPBB 18

FACTORS AFFECTING AGROBACTERIUM TUMEFACIENS MEDIATED GENETIC

TRANSFORMATION OF SOYBEAN CULTIVAR NARC-7.
NEELAM TAJ1, MUHAMMAD ZIA3, YAMIN BIBI1*, NAVEED IQBAL1, M FAYYAZ CHAUDHARY2
1

Department of Botany, PMAS Arid Agriculture University Rawalpindi Pakistan

2
Department of Microbiology, Quaid-i-Azam University Islamabad Pakistan
3
Department of Biotechnology, Quaid-i-Azam University Islamabad Pakistan
*
Corresponding authors email: [email protected]

The aim of study was to optimize some transformation conditions of the soybean cultivar NARC-7 with
Agrobacterium tumefacians strain LBA4404 containing plasmid pLBRRolABCnptII. It was observed that
Agrobacterium tumfaciens concentration at OD 1.0 at pH 5.4 at time of infection resulted maximum transformation
efficiency. Shaking of culture and explants during infection also increased transformation efficiency many folds.
Morphologically rolABC soybean transformants were dwarf with short internodal distance and wrinkled leaves.
However, the transformed plants had long and profused roots as compared to control non-transformed plants.
OPBB 19

BAC DERIVED NEW SSRS FOR USE IN COTTON (GOSSYPIUM SPP) IMPROVEMENT.
NABILA TABBASAM1, YUSUF ZAFAR1, MEHBOOB-UR-RAHMAN1* AND ANDREW H PATERSON2
1

Plant Genomics & Molecular Breeding Lab, National Institute for Biotechnology & Genetic Engineering (NIBGE),
Jhang Road Faisalabad Pakistan
2
Plant Genome Mapping Laboratory, University of Georgia, Athens, Georgia 30602, USA.
*
Corresponding author: [email protected]

A new set of SSRs with di-, tri-, tetra-, penta- and hexa-nucleotide repeats were fished out from bacterial artificial
chromosome (BAC) ends and BAC clone sequences of Gossypium raimondii. A total of 1303 SSRs were designed: 766
from BAC end sequences and 537 from BAC clone sequences. These primer pairs were named as PR-GR-BESS and PRGR-BS (PR for the last name of the both principal investigators, GR for Gossypium raimondii, BES for BAC end
sequences, B for BAC clone and S for simple sequence repeat). Now onward these will be called as BAC-gSSRs in this
manuscript. This new set of G. raimondii derived BAC-SSRs were tested for their transferability to other important
cotton genomes including cultivated tetraploids (G. hirsutum and G. barbadense) and diploid ancestral species (G.
raimondii and G. arboreum). In the present study, G. raimondii was used as a positive control for PCR amplification.
BAC-gSSRs contained diverse types of repeat motifs. We identified 1303 BAC-gSSRs, out of these 731 were
dinucleotide (56.10%), followed by tri (397, 30.46%), tera (118, 9.05%), hexa (40, 3.06%) and penta (17, 1.30%). Hexa
nucleotide repeats had shown the highest level of polymorphism (42.4%) followed by penta (35.29%), tetra (30.50%), tri
(23.25%) and di (19.0%). More than 30 % of the SSRs amplified two fragments, separated in high resolution agarose
metaphor gel electrophoresis. A total of 30% of the total primers were unable to amplify clear fragments in G. arboreum
species. These primers produced some private alleles in G. raimondii and AD genome species indicating the specificity
of these SSRs for D genome. For genetic diversity assessment, PIC values were calculated. Average PIC value was 0.39
with a range of 0.12 to 0.85. G. arboreum was found more close to G. barbadense (0.63) as compared to G. hirsutum 0.57
while G. raimondii showed equal genetic similarity with both the G. hirsutum (0.59) and G. barbadense (0.59). This
study will be instrumental in developing dense genetic maps, and also initiating initiate marker-assisted selection (MAS)
in cotton.
OPBB 20

GENETIC DIVERGENCE AMONG PAKISTANI BREAD WHEAT VARIETIES AND ADVANCED

LINES FOR RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS
RAHEELA REHMAN1, KAUSAR NAWAZ SHAH2, M. SHAHID MASOOD1, MUHAMMAD ARSHAD1, M.
FAHIM ABBAS1 AND ABDUL GHAFOOR1
1

National Agricultural Research Centre (NARC), Park Road, Islamabad, PAKISTAN,

Department of Plant Breeding and Genetic PMAS-Arid Agricultural University, Rawalpindi, PAKISTAN
*
Corresponding authors email: [email protected]

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59

A large number of wheat varieties have been bred over the years but very few have been investigated for genetic
divergence at molecular level. The present paper explains the genetic diversity for Randomly Amplified Polymorphic DNA
(RAPD) markers among wheat varieties as well as advanced lines. 20 RAPD decamer primers were used to determine the
extent of genetic differences among 48 genotypes and half of the primers were monomorphic and other 10 generated 71
DNA fragments with an average of about 7.1 bands per primer. The primer OPE-01, OPB-13 and OPB-09 have 17%, 14%
and 5.6% shared to the total polymorphism among total variation. Maximum 47 genotypes were amplified with the primer
OPA-09 and minimum 11 with OPA-16. 6 genotypes NR-346, NR-373, NR-389, NR-383, WSP-148 and WSP-196 were
the most diverse from rest of the genotypes for RAPD analysis. The information about genetic similarity and differences
will be helpful to avoid any possibility of elite germplasm becoming genetically uniform.
OPBB 21

DEVELOPMENT AND CHARACTERIZATION OF BARLEY CORE COLLECTION: A

STRATEGY FOR GERMPLASM MANAGEMENT
REHAN NAEEM1,2 AND BUSHRA MIRZA1*
1

Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology (KUST), Pakistan.
*
Corresponding authors email: [email protected]

Redundant materials in germplasm collections cause hurdles in gene bank management. Present study was
conducted to analyze redundancies using Simple Sequence Repeats (SSRs) and to evaluate genetic diversity of barley
germplasm comprising of 404 Hordeum vulgare landraces belonging to 8 different regions (Pakistan. India, Iran, Nepal,
Iraq, Turkmenistan, Uzbekistan Kazakhstan). In total 50 alleles were detected. Number of alleles observed was highest
for Bmag0023 (11) followed by HVLOX (9), HVM54 (8), Bmag0382 (7), Bmag0500 (5), Bmag0490 (5) and HVID (5)
locus. Allele size range was largest for HVLOX while it was lowest for Bmag0500. Allele frequencies were found
highest for allele HVM54-120 (0.39) followed by HVID-80 (0.38) and Bmag0382-66 (0.35).
Core collection for the barley germplasm comprised of 42 patterns including 14 unique and 28 frequent patterns.
The number of patterns observed in landraces of different regions was in the order of Pakistan > India > Nepal >
Turkmenistan > Iraq > Iran > Uzbekistan > Kazakhstan. These patterns were clearly clustered into two groups using
Jaccards cluster analysis (group A and group B). Principal Component Analysis (PCA) also distributes these patterns
into three groups. The overall results revealed that differentiation of patterns using Jaccards cluster analysis was in
accordance with PCA which revealed that these patterns were distributed with respect to their geographic regions.
Furthermore, it was concluded that development of core collection is the one strategy besides other strategies to reduce
redundancies in gene bank management and make the handling of germplasm easier.
OPBB 22

EVALUATION OF CARALLUMA TUBERCULATA PLANT AND CALLUS

EXTRACTS AS FREE RADICAL SCAVENGER
RIAZ-UR-REHMAN1, GAUNG LU2, ABDUL MANNAN3, MUHAMMAD FAYYAZ CHAUDHARY4
AND MUHAMMAD ZIA5*
1

Department of Horticulture and Floriculture, Government of Punjab, Rawalpindi, Pakistan

2
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou China
3
Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan
4
Department of Nano Technology, Preston University Islamabad, Pakistan
5
Department of Biotechnology, Quaid-I-Azam University, Islamabad 45320, Pakistan
*Corresponding author email: [email protected]; Phone 92-51-9064-4126
Caralluma tuberculata (Asclepediaceae) is a potent medicinal pant, traditionally used against sundry diseases
including diabetes. In current study, Caralluma tuberculata fresh plant and callus were extracted with solvents of
different polarities and extracts were evaluated for the presence of flavonoids, phenolics, DPPH scavenging activity and
reducing power. As per results, flavonoids percentage in plant and callus extract was 0.092% and 0.039%, respectively.
In case of fractions, highest yield flavonoids were recovered in water fractions of both plant and callus extracts i.e. 0.049
% and 0.021%, respectively. The phenolics compounds percentage in plant and callus extract was calculated 0.16% and
0.057%, respectively. In further fractions, maximum phenolics compounds were harvested from water fraction of plant
and callus. Extract of water also demonstrated the highest DPPH scavenging activity with EC50 of 4.95 mg/ml after 30
min followed by the n-butanol fraction which exhibit EC50 value 8.30 mg/ml after 30 min. The reducing power assay
comprised doze dependent response and water fraction demonstrated the highest reducing power activity in both plant

BIOTECHNOLGY, BIOCHEMISTRY AND BIOINFORMATICS

60

and callus water fraction (optical density 0.729 and 1.232 at 10 and 20 mg/ml, respectively in plant and 0.595 and 0.619,
respectively in callus). The greater amount of flavonoids, phenolic compounds and reducing power leads to more potent
radical scavenging effect as shown by Caralluma tuberculata plant and callus extract. Current study supports the use of
both plant and callus bodies of Caralluma tuberculata as sources of natural antioxidant compounds.
OPBB 23

MOLECULAR IDENTIFICATION AND COMPARATIVE ANALYSIS OF NOVEL 18S

RIBOSOMAL RNA GENOMIC SEQUENCES OF WIDE RANGE OF WILD MEDICINAL PLANTS
SADIA BANARAS1, SITWAT AMAN2, MARYAM ZAFAR2, MUDASSAR KHAN2,
SAFDAR ABBAS2, ZABTA KHAN SHINWARI1 AND SAMINA N SHAKEEL*2
1

Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University 45320, Islamabad, Pakistan
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University 45320, Islamabad, Pakistan

Ribosomal RNAs (rRNAs) are universally distributed and known for their functional equivalence among all the known
organisms. Analysis of small-subunit rRNAs (16-18S rRNAs) can permit the accurate statistical measurement of a broad
range of phylogenetic relationships due to highly conserved sequences. Therefore, we identified and partially sequenced
novel isoforms of 18S rRNA gene from ten diverse wild medicinal plants including Ferocactus glaucescens, Solanum
Lycopersicum, Capparis deciduas, Calatropis procera, Cyamopsis tetragonoloba, Eruca sativa, Maytenus royleana,
Prosopis Juliflora, Ficus carica and Mentha spicata. Sequences were submitted to genebank followed by sequence analysis
and confirmation by using bioinformatics tools. We used ClustalW for pairwise alignment of these novel sequences with
other known 18S rRNA sequences to find out their phylogenetic relationships. Our results have shown highly conserved
nature of 18S rRNA with variable regions might be indications of some historical signals. Secondary structure constrains of
rRNA can affect their phylogenetic interpretations rarely. These novel 18S rRNA sequences can also be used as internal
controls for several types of molecular analysis after accurate validations of their consistent expression in the given plant
species in future studies, as less is known about these housekeeping genes of wild plants.
OPBB 24

PROTEOMIC AND TRANSCRIPTOMIC APPROACH TO EXPLORE THE MOLECULAR

BASIS OF ADAPTATION OF AGAVE AMERICANA TO HEAT STRESS
SAMINA N SHAKEEL1, 2*, SITWAT AMAN1, NOOR UL HAQ1, SCOTT A. HECKATHORN3
AND DAWN LUTHE4
1

Department of Biochemistry, Faculty of Biological Sciences,Quaid-i-Azam University,Islamabad,Pakistan

2
Department of Biochemistry and Molecular Biology, Mississippi State University, MS, USA
3
Department of Environmental Sciences, University of Toledo, Toledo, OH, USA
4
Department of Crop and Soil Sciences, College of Agricultural Sciences, Pennsylvania State University, USA
Crassulacean Acid Metabolism (CAM) plants have to cope with different environmental stresses including heat,
water and/or CO2-limited environments throughout their lifecycles. Heat stress affects the rate of the photosynthesis and
related thermotolerance in many plants. To elucidate heat tolerance mechanism and role of stromal proteome in a heat
tolerant plant Agave americana, we used a novel proteomics approach; Multidimensional Protein Identification
Technology (MudPIT) followed by mass spectrometry (MS). Stromal proteins were extracted from heat stressed and
control plants. Several differentially expressed stromal proteins were identified under heat stress followed by their in
silico subcellular localization, biological and molecular functions. We identify 58 stromal proteins that that can play
important role in photosynthesis, defense, plastid metabolic functions, hormonal biosynthesis, stress signal perception
and transduction. Both nuclear and chloroplast encoded proteins decreased under heat stress suggesting disruption of
physiological and metabolic pathways at high temperature stress. Similarly relative transcript levels of 16 selected
stromal genes were analyzed. Both, MudPIT and real-time PCR analyses have shown up or down regulation of several
proteins under heat stress. Absence of correlation between some transcriptomic and proteomics data revealed significant
role of post-translational modifications in Agave thermotolerance. Taken together, our results provide the first extensive
picture of proteomic and transcriptomic comparisons of stroma (chloroplast) of CAM plant under heat stress.

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61

OPBB 25

INTEGRATION OF NOVEL CHLOROPHYLL GENES FROM BLACK PINE

INTO THE CHLOROPLAST GENOME OF TOBACCO
SHAHID NAZIR1 AND MUHAMMAD SARWAR KHAN2*
2

1,2
National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road Faisalabad, Pakistan,
Center of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad Pakistan
*
Corresponding authors email: [email protected]

Gymnosperms owing the presence of dark-operative protochlorophyllide oxidoredcuatse pathway are able to reduce
protochlorophyllide to chlorophyllide leading to the formation of chlorophyll in dark, whereas angiosperms are unable to
do this and are less photosynthetically efficient. Plastid encoded genes chlL and chlN are reported to be involved in the
functioning of the enzyme Dark-operative Protochlorophyllide Oxidoreductase (DPOR). The genes were isolated from
black pine and cloned into the species-specific chloroplast targeting vector; harboring FLARE-S, a fluorescent selection
marker. The final transformation vector was used to introduce genes into the tobacco chloroplast genome using biolistic
approach. Here, we report stable integration of both genes along with translationally fused marker genes (gfp and aadA)
into the inverted repeat region of plastome which was confirmed by Polymerase Chain Reaction and Southern blot
analysis. Morphological and physiological analyses of the transgenic plants compared with non-transformed wild type
tobacco plants revealed that the activation of dark-operative pathway requires additional factors/genes to chlL and chlN
genes to develop chlorophyll, and consequently photosynthetically competent chloroplasts.
OPBB 26

ALTERNATE RESISTANCE GENE AGAINST YELLOW RUST IN WHEAT

SHAHZADI FAIZA SAFDAR AND HAMID RASHID*
Department of Bioinformatics, Mohammad Ali Jinnah University, Islamabad, Pakistan
*
Corresponding authors email: [email protected]
Yellow rust, also known as stripe rust, is one of the three wheat rust diseases principally found in wheat grown in
cooler environments. Yellow rust, caused by Puccinia striiformis Westend. f.sp. tritici, is an important foliar disease of
wheat, and development resistant cultivars are the most economical method of control. Of the three rust diseases of
wheat, stripe (yellow) rust (YR) is the most damaging to grain yields in cool, moist environments. The minimum,
optimum, and maximum temperatures for spore germination are 0oC, 9-12oC, and 20-26oC respectively. This disease
was first discovered by Gadd in 1977, but proper identification as another rust-type disease was done by Eriksson and
Henning in 1986.Chemical control of rusts is expensive and hazardous to the environment. Yr5 and Yr10 are resistance
genes among many other resistance genes while PsMAPK1 is fungus protein causing yellow rust in wheat. The protein
sequences of Yr5 and Yr10 was retrieved from NCBI and PsMAPK1 protein sequence was retrieved from UNIPROT.
By the help of I-TASSAR 3D models of Yr5, Yr10 and PsMAPK1 were generated. The models were then validated by
the help of Ramachandaran plot assessment and then models were evaluated by energy minimization. After model
evaluation docking was performed to find the alternate resistance gene from the Yr5 and Yr10 templates.The docking
results shows the alternate gene which can be used as the alternate source to cure this disease in wheat. The 3D models
show that how these resistance genes interact with the fungus proteins.
OPBB 27

GENETIC FINGERPRINTING OF LOCAL TURMERIC GENOTYPES USING RAPDS

SIFFATULLAH KHAN, SHAMA NAZ, REEMA AND REHAN NAEEM*
Department of Biotechnology and Genetic Enginee