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The document describes an experiment to prepare and evaluate topical suspensions with different humectants including glycerin, propylene glycol, and sorbitol. Precipitated sulfur was used as the active ingredient. The suspensions were characterized based on appearance, pH, sedimentation parameters, viscosity, particle size distribution, spreadability, and occlusivity testing to measure transdermal water loss. Glycerin at 30% concentration provided the best occlusivity with lowest water loss through skin. The humectants help maintain skin moisture and prevent diseases by forming an occlusive layer and absorbing water from the surroundings.

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85 views9 pages

Date Page No

The document describes an experiment to prepare and evaluate topical suspensions with different humectants including glycerin, propylene glycol, and sorbitol. Precipitated sulfur was used as the active ingredient. The suspensions were characterized based on appearance, pH, sedimentation parameters, viscosity, particle size distribution, spreadability, and occlusivity testing to measure transdermal water loss. Glycerin at 30% concentration provided the best occlusivity with lowest water loss through skin. The humectants help maintain skin moisture and prevent diseases by forming an occlusive layer and absorbing water from the surroundings.

Uploaded by

kjghlkdfjg
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Date

Page no.

PRACTICAL-13
AIM- To Prepare and Evaluate Stable topical Suspension Of given
API & study effect of different humectants.
FormulaEach 5 ml of topical suspension contains API Precipitated sulphur
Excipients - q.s.,

Batch size- 40 ml
Packaging- Narrow mouth glass bottle with metal screw cap.
FormulationFORMULA:
Ingredients

Precipitated
sulphur
Humectant

Quantity
for 30 ml
1.5 gm
1.5 ml

Sodium lauryl 0.075 gm


Sulphate
Alcohol (95%) 4.5 ml
Rose Water
30 ml

Quantity for 40 ml
Glycerine
4.5 gm

PG
4.5 gm

Sorbitol
4.5 gm

15%(4.5
ml)
0.075

10%(3
ml)
0.075

12%(3.6
ml)
0.075

humectant

4.5
26.42 ml

4.5
27.92 ml

4.5
27.32 ml

co solvent
Vehicle

Ideal Humectant Concentration:


Glycerin-30%
Propylene Glycol-15%
Sorbitol-3-15%

Role of ingredients

API

Suspending agent

Date

Page no.

Observations:
1. Appearance2. pH value:
Humectant
pH

Glycerine

PG

Sorbitol

3. Sedimentation Parameters:
a.) Sedimentation volume ratio
Humectant Time(min)
Initial
height(ml)
Glycerine
10
20
30
40
50
60
PG

10
20
30
40
50
60

Sorbitol

10
20
30
40
50
60

Final
height(ml)

Ratio

Date

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METHOD OF PREPARATION-

Ingredients such as zinc stearate, sodium lauryl sulphate were weighed and
transferred into mortar.
Humectant and alcohol were added as per quantity into mortar.
They all were triturated with continuous stirring with pastel.
Rose Water was added as quantity sufficient and mixed uniformly.
Prepared suspension was transferred to glass bottles.
Labelling was done.

Method of characterization1. Appearance


2. pH value
3. Sedimentation parameters
a.)Sedimentation volume ratio
b.)Sedimentation rate
c.)Redispersibility
4. Viscosity
5. Particle size distribution
6. Spreadbility
7. Occlusivity Test for water transdermal loss:

Specified volume of water was taken in preweighed bottle.


Formulation was applied on whattmann filter paper which permeable to
water.
Bottle was covered with cap.
At regular interval bottle was weighed & loss of water was determined.

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b.)Sedimentation rate:
Humectants
Glycerin
PG
Sorbitol

Sedimentation rate

4. Redispersibility:
Humectants

[Link] strokes

Glycerine
PG
Sorbitol

5. viscosity: [Brooke field viscometer]


Humectants

Rpm

Centipoises

Glycerin
PG
Sorbitol

6. Particle size distribution:


Factor calculation:

Humectant

Size range

Mean size

[Link]
particles(f)

fd

Date

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COMMENT:
Humectant plays an important role in maintaining skin moisture. It prevents loss of
moisture from skin by forming occlusive layer on skin.
Skin moisture is essential in preventing skin dryness & prevent occurrence of
diseases like psoriasis, dermatitis, etc. The main objective of humectant is to
absorb water from high humidity surrounding atmosphere.
Various humectants like sorbitol, propylene glycol, glycerine etc. are used as
humectant in topical suspension formulations which maintains Occlusivity of skin
by preventing transdermal water loss.
In present study, Occlusivity test was done by using these humectants & results
were affected by various humectants with different concentration.

Date

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Humectant

Size range

Mean size

[Link]
particles(f)

Fd

Humectant

Size range

Mean size

[Link]
particles(f)

Fd

Humectant

Size range

Mean size

[Link]
particles(f)

Fd

Date

Page no.

REFERENCEPharmaceutical Dosage Forms: Disperse System Vol-2; Second Edition,


[Link], Leon Lachman and Joseph [Link]; Pg no.205,423

Date

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7. Spreadibility
Humectant

Spreadbility

Glycerine
PG
Sorbitol

[Link]
Humectant
Glycerin
PG
Sorbitol

Humectant

Glycerin
PG
Sorbitol

Weight of bottle

After 3 days

After 5 days

% Transdermal water loss

Date

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