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Microscope

Dark field microscopy uses a technique where the light source is blocked off, allowing light to scatter and produce high-contrast images of transparent samples. A phase contrast microscope uses an optical mechanism to make otherwise transparent objects appear bright against a dark background. A fluorescence microscope uses fluorescence instead of reflection or absorption to study organic and inorganic substances. A confocal microscope increases resolution and contrast by using point illumination and a pinhole to eliminate out-of-focus light. An atomic force microscope is a very high-resolution type of scanning probe microscopy that can image at the nanometer scale.

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566 views1 page

Microscope

Dark field microscopy uses a technique where the light source is blocked off, allowing light to scatter and produce high-contrast images of transparent samples. A phase contrast microscope uses an optical mechanism to make otherwise transparent objects appear bright against a dark background. A fluorescence microscope uses fluorescence instead of reflection or absorption to study organic and inorganic substances. A confocal microscope increases resolution and contrast by using point illumination and a pinhole to eliminate out-of-focus light. An atomic force microscope is a very high-resolution type of scanning probe microscopy that can image at the nanometer scale.

Uploaded by

angelabrojo
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd

Dark Field Microscopy

DEFINITION arranged so that the light source is blocked off, causing light to scatter as it hits the specimen a contrast-enhancing optical technique that can be utilized to produce highcontrast images of transparent specimens, such as living, microorganisms, thin tissue slices, lithographic patterns, fibers, latex dispersions, glass fragments, and subcellular particles an optical microscopy illumination technique used to enhance the contrast in unstained, transparent samples

Phase Contrast Microscope

USES ideal for making objects with refractive values similar to the background appear bright against a dark background employs an optical mechanism to translate minute variations in phase into corresponding changes in amplitude, which can be visualized as differences in image contrast

Differential Interference Contrast

Fluorescence Microscope

an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances

Confocal Microscope

Scanned Probe Microscopy

Atomic Force Microscope

optical imaging technique used to increase optical resolution and contrast of a micrograph by using point illumination and a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane branch of microscopy that forms images of surfaces using a physical probe that scans the specimen very high-resolution type of scanning probe microscopy, with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the optical diffraction limit

separating a polarized light source into two orthogonally polarized mutually coherent parts which are spatially displaced (sheared) at the sample plane, and recombined before observation uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope, or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescent image enables the reconstruction of three-dimensional structures from the obtained images

can image several interactions simultaneously consists of a cantilever with a sharp tip (probe) at its end that is used to scan the specimen surface

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