NUTRIENT AGAR 1.
5% (7286)
Intended Use Nutrient Broth 1.5% is used for the cultivation of a wide variety of microorganisms. Product Summary and Explanation In the early 1900s, the American Public Health Association (APHA) suggested the formula of Nutrie nt Agar 1 as a standard culture medium used in water testing. Nutrient Agar is specified in many standard method 2-6 procedures. Nutrient Agar 1.5% is a modification of Nutrient Agar. Nutrient Agar 1.5% is a general purpose medium, with a slightly alkaline pH. This medium contains 0.8% sodium chloride and can be used as a base for enrichment with blood, ascitic fluid, or other supplements for cultivating fastidious microorganisms. Principles of the Procedure The nitrogen, carbon, vitamins, and amino acids are provided by Enzymatic Digest of Gelatin and Beef Extract. Sodium Chloride maintains the osmotic balance of the medium so red blood cells will not rupture 2 when the medium is supplemented with blood. Agar is the solidifying agent. Formula / Liter Beef Extract .............................................................................. 3 g Enzymatic Digest of Gelatin ...................................................... 5 g Sodium Chloride ....................................................................... 8 g Agar ........................................................................................ 15 g Final pH: 7.3 0.2 at 25C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Precautions 1. For Laboratory Use. 2. IRRITANT. Irritating to eyes, respiratory system, and skin. Directions 1. Suspend 31 g of the medium in one liter of purified water. 2. Heat with frequent agitation and boil for one minute to completely dissolve the medium. 3. Autoclave at 121C for 15 minutes. Quality Control Specifications Dehydrated Appearance: Powder is homogeneous, free flowing, and beige. Prepared Appearance: Prepared medium is trace to slightly hazy and beige. Expected Cultural Response: Cultural response on Nutrient Agar 1.5% incubated aerobically at 35 2C and examined for growth after 18 - 24 hours. Microorganism Bacillus subtilis ATCC 9372 Escherichia coli ATCC 25922 Salmonella typhimurium ATCC 14028 Staphylococcus aureus ATCC 25923 Streptococcus pneumoniae ATCC 6305 Streptococcus pyogenes ATCC 19615 Approx. Inoculum (CFU) 10 - 300 10 - 300 10 - 300 10 - 300 10 - 300 10 - 300 Expected Results Growth Growth Growth Growth Growth Growth
The organisms listed are the minimum that should be used for quality control testing.
PI 7286, Rev 2, April 2011
Test Procedure For a complete discussion on the isolation and identification of aerobic and anaerobic microorganisms, refer to appropriate references. Results Refer to appropriate references and procedures for results. Storage Store sealed bottle containing the dehydrated medium at 2 - 30C. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed. Expiration Refer to expiration date stamped on the container. The dehydrated medium should be discarded if not free flowing, or if appearance has changed from the original color. Expiry applies to medium in its intact container when stored as directed. Limitation of the Procedure Due to nutritional variation, some strains may be encountered that grow poorly or fail to grow on this medium. Packaging Nutrient Agar 1.5%
Code No.
7286A 7286B 7286C
500 g 2 kg 10 kg
References
1. 2. 3. 4. 5. 6.
American Public Health Association. 1917. Standard methods of water analysis, 3rd ed. American Public Health Association, Washington, D.C. Vanderzant, C., and D. F. Splittstoesser (eds.). 1992. Compendium of methods for the microbiological examination of foods, 3 rd ed. American Public Health Association, Washington, D.C. Eaton, A. D., L. S. Clesceri, and A. E. Greenberg (eds.). 1995. Standard methods for the examination of water and wastewater, 19th ed. American Public Health Association, Washington, D.C. Marshall, R. T. (ed.). 1993. Standard methods for the microbiological examination of dairy products, 16th ed. American Public Health Association, Washington, D.C. Association of Official Analytical Chemists. 1995. Official methods of analysis of AOAC International, 16th ed. AOAC International, Arlington, VA. www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalmanualBAM/default.htm.
Technical Information
Contact Acumedia Manufacturers, Inc. for Technical Service or questions involving dehydrated culture media preparation or performance at (517)372-9200 or fax us at (517)372-2006.
PI 7286, Rev 2, April 2011
