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Understanding DNA Microarrays Explained

The ability to label nucleic acid molecules and then use them to interrogate other nucleic acids attached to a solid support was first described over 25 years ago. This technique, eponymously named Southern blotting, uses a DNAprobe labelled with either a radioisotope or a fluorescent tag. The probe is applied to the fragment of DNAor RNAto be studied and by the rules of base pairing (Ato T, C to G) will ''stick''

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37 views1 page

Understanding DNA Microarrays Explained

The ability to label nucleic acid molecules and then use them to interrogate other nucleic acids attached to a solid support was first described over 25 years ago. This technique, eponymously named Southern blotting, uses a DNAprobe labelled with either a radioisotope or a fluorescent tag. The probe is applied to the fragment of DNAor RNAto be studied and by the rules of base pairing (Ato T, C to G) will ''stick''

Uploaded by

DrKrishna Das
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

What is a DNA microarray?

The ability to label nucleic acid molecules and then use them to interrogate other nucleic acids attached to a solid support was first described over 25 years ago. This technique, eponymously named Southern blotting, uses a DNAprobe labelled with either a radioisotope or a fluorescent tag. The probe is applied to the fragment of DNAor RNAto be studied and by the rules of base pairing (Ato T, C to G) will stick to its complementary sequence. Indeed it has been described as the first type of array.5 Advances in technology have made it possible to miniaturise methods of probe detection for DNAand allow detection of several thousand DNAor RNAsequences in one experiment. The process is the reverse of Southern blotting, the probe being placed on an immobile surface and then exposed to the free nucleic acid to be analysed, known as the target . The application of the chemical process is not new; it is the scale on which it can be used that has made it such an astounding success.

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