Based on Naegleria/Acanthamoeba E.
histolytica
Nucleolus Large and distinct Small and indistinct
Contractile vacuoles present Absent
Nucleus in cyst single 4 in mature cyst
Glycogen and chromatoid bodies absent present
(in cyst)
Mitochondria present Absent
Cyst wal May have pores or ostrioles No pores
Morphological differences of Nagleria and Acanthamoeba
Naegleria Acanthamoeba
Trophozoite with broad pseudopods With filamentous pseudopods
Actively motile Sluggishly motile
Forms flagellate stage Does not form flagellate stage
Single walled cyst Double walled
Does not encyst in tissue May encyst in tissue