Cloning vector pUC19, complete sequence
GenBank: M77789.2
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LOCUS
AUG-2008
DEFINITION
ACCESSION
VERSION
KEYWORDS
SOURCE
ORGANISM
REFERENCE
AUTHORS
TITLE
JOURNAL
PUBMED
REFERENCE
AUTHORS
TITLE
nucleotide
JOURNAL
PUBMED
REMARK
REFERENCE
AUTHORS
TITLE
JOURNAL
Univ., 190
REFERENCE
AUTHORS
TITLE
JOURNAL
Univ., 190
SYNPUC19V
2686 bp
DNA
circular SYN 07-
Cloning vector pUC19, complete sequence.
M77789 M11662
M77789.2 GI:6691170
.
Cloning vector pUC19
Cloning vector pUC19
other sequences; artificial sequences; vectors.
1 (bases 1 to 2686)
Norrander,J., Kempe,T. and Messing,J.
Construction of improved M13 vectors using
oligodeoxynucleotide-directed mutagenesis
Gene 26 (1), 101-106 (1983)
6323249
2 (bases 1 to 2686)
Yanisch-Perron,C., Vieira,J. and Messing,J.
Improved M13 phage cloning vectors and host strains:
sequences of the M13mp18 and pUC19 vectors
Gene 33 (1), 103-119 (1985)
2985470
Erratum:[Gene. 1992 May 1;114(1):81-3. PMID: 1375182]
3 (bases 1 to 2686)
Messing,J.
Direct Submission
Submitted (27-SEP-1991) Waksman Institute, Rutgers State
Frelinghuysen Road, Piscataway, NJ 08854, USA
4 (bases 1 to 2686)
Messing,J.
Direct Submission
Submitted (12-JAN-2000) Waksman Institute, Rutgers State
Frelinghuysen Road, Piscataway, NJ 08854, USA
REMARK
Sequence update by submitter
COMMENT
On Jan 12, 2000 this sequence version replaced gi:209214.
FEATURES
Location/Qualifiers
source
1..2686
/organism="Cloning vector pUC19"
/mol_type="other DNA"
/strain="ATCC 37254"
/culture_collection="ATCC:37254"
/db_xref="taxon:31851"
misc_feature
1..447
/note="M13mp19"
misc_feature
1..230
/note="Lac-operon"
misc_feature
233..289
/note="polylinker of M13mp19;
HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacIEcoRI"
misc_feature
misc_feature
misc_feature
misc_feature
290..447
/note="Lac-Operon"
complement(448..547)
/note="pBR322"
complement(548..684)
/note="pBR322"
complement(685..2686)
/note="pBR322"
ORIGIN
1 gcgcccaata
gcagctggca
61 cgacaggttt
tgagttagct
121 cactcattag
tgtgtggaat
181 tgtgagcgga
ccaagcttgc
241 atgcctgcag
ctggccgtcg
301 ttttacaacg
cttgcagcac
361 atcccccttt
ccttcccaac
421 agttgcgcag
acgcatctgt
481 gcggtatttc
gccgcatagt
541 taagccagcc
tgtctgctcc
601 cggcatccgc
cagaggtttt
661 caccgtcatc
tttttatagg
721 ttaatgtcat
ggaaatgtgc
781 gcggaacccc
ctcatgagac
841 aataaccctg
attcaacatt
901 tccgtgtcgc
gctcacccag
961 aaacgctggt
ggttacatcg
1021 aactggatct
cgttttccaa
1081 tgatgagcac
gacgccgggc
1141 aagagcaact
tactcaccag
1201 tcacagaaaa
gctgccataa
1261 ccatgagtga
ccgaaggagc
1321 taaccgcttt
tgggaaccgg
cgcaaaccgc ctctccccgc gcgttggccg attcattaat
cccgactgga aagcgggcag tgagcgcaac gcaattaatg
gcaccccagg ctttacactt tatgcttccg gctcgtatgt
taacaatttc acacaggaaa cagctatgac catgattacg
gtcgactcta gaggatcccc gggtaccgag ctcgaattca
tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
acaccgcata tggtgcactc tcagtacaat ctgctctgat
ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
accgaaacgc gcgagacgaa agggcctcgt gatacgccta
gataataatg gtttcttaga cgtcaggtgg cacttttcgg
tatttgttta tttttctaaa tacattcaaa tatgtatccg
ataaatgctt caataatatt gaaaaaggaa gagtatgagt
ccttattccc ttttttgcgg cattttgcct tcctgttttt
gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
caacagcggt aagatccttg agagttttcg ccccgaagaa
ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
cggtcgccgc atacactatt ctcagaatga cttggttgag
gcatcttacg gatggcatga cagtaagaga attatgcagt
taacactgcg gccaacttac ttctgacaac gatcggagga
tttgcacaac atgggggatc atgtaactcg ccttgatcgt
1381 agctgaatga
gcaatggcaa
1441 caacgttgcg
caacaattaa
1501 tagactggat
cttccggctg
1561 gctggtttat
atcattgcag
1621 cactggggcc
gggagtcagg
1681 caactatgga
attaagcatt
1741 ggtaactgtc
cttcattttt
1801 aatttaaaag
atcccttaac
1861 gtgagttttc
tcttcttgag
1921 atcctttttt
ctaccagcgg
1981 tggtttgttt
ggcttcagca
2041 gagcgcagat
cacttcaaga
2101 actctgtagc
gctgctgcca
2161 gtggcgataa
gataaggcgc
2221 agcggtcggg
acgacctaca
2281 ccgaactgag
gaagggagaa
2341 aggcggacag
agggagcttc
2401 cagggggaaa
tgacttgagc
2461 gtcgattttt
agcaacgcgg
2521 cctttttacg
cctgcgttat
2581 cccctgattc
gctcgccgca
2641 gccgaacgac
//
agccatacca aacgacgagc gtgacaccac gatgcctgta
caaactatta actggcgaac tacttactct agcttcccgg
ggaggcggat aaagttgcag gaccacttct gcgctcggcc
tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
agatggtaag ccctcccgta tcgtagttat ctacacgacg
tgaacgaaat agacagatcg ctgagatagg tgcctcactg
agaccaagtt tactcatata tactttagat tgatttaaaa
gatctaggtg aagatccttt ttgataatct catgaccaaa
gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
gccggatcaa gagctaccaa ctctttttcc gaaggtaact
accaaatact gttcttctag tgtagccgta gttaggccac
accgcctaca tacctcgctc tgctaatcct gttaccagtg
gtcgtgtctt accgggttgg actcaagacg atagttaccg
ctgaacgggg ggttcgtgca cacagcccag cttggagcga
atacctacag cgtgagctat gagaaagcgc cacgcttccc
gtatccggta agcggcaggg tcggaacagg agagcgcacg
cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
gttcctggcc ttttgctggc cttttgctca catgttcttt
tgtggataac cgtattaccg cctttgagtg agctgatacc
cgagcgcagc gagtcagtga gcgaggaagc ggaaga
Cloning vector pUC19, complete sequence
GenBank: M77789.2
GenBank Graphics
>gi|6691170|gb|M77789.2|SYNPUC19V Cloning vector pUC19, complete sequence
GCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTT
CCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGG
CTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAA
CAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAG
CTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGC
CTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAAC
AGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTC
ACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCG
CCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCG
TCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGT
GATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGAC
AATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC
CCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTG
AGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATT
ATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAA
CCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTT
TTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAAC
TACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCT
GCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGG
CAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTC
AGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTG
AAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACC
CCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAA
AAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACT
GGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA
ACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAA
GTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGG
GGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTAT
GAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGG
AGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTC
TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG
CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTC
TGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGC
GAGTCAGTGAGCGAGGAAGCGGAAGA
