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HPLC Analysis of Caffeine in Soft Drinks

This document provides an introduction to HPLC (High Performance Liquid Chromatography) and its use in determining the concentration of caffeine in soft drinks. It describes the main components of an HPLC system including the mobile phase reservoir, pump, injector, separation column, and detector. It also gives equations for dilution calculations and explains that peak area is proportional to concentration and retention times can be used to identify substances.

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najib
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43 views5 pages

HPLC Analysis of Caffeine in Soft Drinks

This document provides an introduction to HPLC (High Performance Liquid Chromatography) and its use in determining the concentration of caffeine in soft drinks. It describes the main components of an HPLC system including the mobile phase reservoir, pump, injector, separation column, and detector. It also gives equations for dilution calculations and explains that peak area is proportional to concentration and retention times can be used to identify substances.

Uploaded by

najib
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd

HPLC : Determination of caffeine in

soft drink.
Preared by : Mohamad Najib Bin Misri

INTRODUCTION

HPLC is a tool for quantifying and analyzing


the mixture of chemical compounds.
It consist of few different part that carry out
their own different functions
A

reservoir of mobile phase


A pump
An injector
A separation column
A detector

Figure : The Schematic Diagram of HPLC


Source: [Link]

THEORY

To perform dilution from prepared 1000 ppm caffeine standard


solution we need to use this equation:
M1V1 = M2V2

The area of the peak is proportional to the concentration of that


particular species in sample.

The standard identity can be used as a comparison with the


experimental one. Identical substances will have its own peak as
well as retention times.

Figure 2: The Graph of Species Present at Sample in Terms of Absorbance


Source: [Link]

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