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Microbiology E-test Guide

This document provides instructions for performing E-tests to determine antibiotic minimum inhibitory concentrations (MICs) for various bacteria. It lists the appropriate incubation conditions, media, and incubation times for different bacteria. It also provides guidance on when to perform E-tests for specific antibiotics and bacterial groups, such as routinely testing gonorrhea isolates or testing Enterobacteriaceae and Pseudomonas for resistance to meropenem. Instructions are given on reading and interpreting the E-test results.

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suryabratabanerjee
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125 views2 pages

Microbiology E-test Guide

This document provides instructions for performing E-tests to determine antibiotic minimum inhibitory concentrations (MICs) for various bacteria. It lists the appropriate incubation conditions, media, and incubation times for different bacteria. It also provides guidance on when to perform E-tests for specific antibiotics and bacterial groups, such as routinely testing gonorrhea isolates or testing Enterobacteriaceae and Pseudomonas for resistance to meropenem. Instructions are given on reading and interpreting the E-test results.

Uploaded by

suryabratabanerjee
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.

Microregistrar.

com E-test (FRCPath revision)


Make 0.5 McF solution in sterile saline (for mucoid colonies make approx 1 McF). Lawn the plate. Let it dry for 5-10 min. Apply the strip within 15 min of lawning. Reading Read when semi-confluent. If no zone intersecting the strip ie very sensitive - report as less than the lowes conc in the strip. If no inhibition - report as more than the highest conc in e-test strip. If MIC falls between markings report the higher value. For haemolytic organism disregard haemolysis, measure the inhibition. Incubating condition

Acinetobacter Anaerobes Campylobacter

MHA

35/air/20h 35/ano2/24h 35/microaerophilic /20h 35/CO2/20h

N gonorrhoeae N meningitidis P multocida

35/co2/20h 35/co2/20h 35/co2/20h

Coryneform Enterobacterice ae Enterococci H influenzae Listeria M catarrhalis MHA

Pseudomonas sp S maltophilia

MHA MHA

35/air/20h 30/air/20h

35/air/20h

35/air/24h 35/co2/20h 35/co2/20h 35/air/20h

S pneumoniae Staphylococci Alpha haemolytic strep Beta haemolytic strep

35/co2/20h 35/air/24h 35/co2/20h 35/air/20h

When to do E test

Cipro, pen Gent Cotri mero Chloramphenicol Teic Gent, ampi, cotri Ampi, Gent - high and low

N gonorrhoeae Moraxella, Haemophilus Stenotrophomonas Enterobactericeae, Pseudomonas BHS, str pneumo CoNS Listeria Alpha HS

Routine, all isolates When topical agents needed Routine, all isolates Resistance to mero on disc When topical agents needed

Routine, all isolate Endocarditis

Microregistrar.com E-test (FRCPath revision)


Mupirocin Staph When high level R suspected

Do not report - irrespective of in-vitro result.

Salmonella and Shigella

1st/2nd gen cephalosporin and aminoglycoside

Enterococci

Cephalosporin, Cotrimoxazole, Clindamycin, Aminoglycoside (Except high level synergy testing result) Cephalosporin Beta - lactams Cephalosporin, Penicillin, Aztreonam

Listeria MRSA ESBL E coli/Klebsiella

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