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AFM Calibration Specimen 607-AFM

This document provides instructions for calibrating an atomic force microscope using a calibration specimen. The specimen is a cellulose acetate replica grating with a line spacing of 0.463μm. To calibrate the microscope's magnification, measure the distance and number of spaces using a scan size under 10μm and use the provided calculation. Handle the specimen carefully by the metal disk and store facedown in the original box to avoid damaging the surface.

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Gaurav Acharya
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166 views1 page

AFM Calibration Specimen 607-AFM

This document provides instructions for calibrating an atomic force microscope using a calibration specimen. The specimen is a cellulose acetate replica grating with a line spacing of 0.463μm. To calibrate the microscope's magnification, measure the distance and number of spaces using a scan size under 10μm and use the provided calculation. Handle the specimen carefully by the metal disk and store facedown in the original box to avoid damaging the surface.

Uploaded by

Gaurav Acharya
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

CALIBRATION SPECIMEN for ATOMIC FORCE MICROSCOPE PRODUCT NO.

607-AFM
This specimen is a cellulose acetate replica of a 2,160 lines/mm waffle pattern diffraction grating. When imaging the specimen, it should be kept in mind that the line spacing is 0.463m and the pattern will not be visible until the imaging system is set to resolve that level of detail.

The height of the bars of the grid pattern is approximately 31nm. To calibrate instrument magnification, use the following calculation where A represents the distance in mm between limiting lines of the spaces measured and B represents the number of spaces measured: Magnification = A x 2,160 / B (Use a scan size of under 10m for best results.) CARE OF THE SPECIMEN Open the storage box by cutting the white label where the lid and base meet. At all times, the surface of the replica must be protected from damage. The replica is mounted on a metal disk, which is laying facedown in the storage box. The disk can be removed by carefully inserting forceps under the rim, using the groove provided in the box base. Do not grip more than 1mm into the center of the disk. Once the disk is removed from the storage box, always lay it down with the specimen side facing up. Store the specimen in the original box or a similar container, which will protect the surface. Do not attempt to clean the specimen or remove it from the metal disk. Never allow the specimen to be exposed to water or solvents. Note: During the mounting process, an area around the extreme outer edges of the specimen may have been damaged. Avoid these areas when using the specimen for calibration purposes. 607-AFM TN 3/02

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