The challenge of multidrug resistance (MDR) in cancer, which hindered successful chemotherapy, was primarily due to the increased activity of drug efflux transporters in cancer cells. This study explored the potential of a nanosized... more
Biotransformation of flavonoid dimer FD18 resulted in an active metabolite FM04. It was more druggable because of its improved physicochemical properties. FM04 (EC50 = 83 nM) was 1.8-fold more potent than FD18 in reversing P-glycoprotein... more
Nguyễn Thanh Tùng, Đặng Văn Phước, Đặng Ngọc Phúc, Đặng Công Thuận, Lê Minh Tâm, Nguyễn Vũ Quốc Huy, Kim Won. 1 Viện Y Sinh, Trường Đại học Y Dược, Đại học Huế 2 Khoa Cơ bản, Trường Đại học Y Dược, Đại học Huế 3 Khoa Y, Trường Đại học... more
A major class of membrane proteins is ABC transporters, which are employed for various activ ities l ike the transportation of substances f rom the exterior to the intracellular environment and vice versa. These proteins require energy in... more
Amyloidosis mouse models of Alzheimer's disease are generally established by transgenic approaches leading to an overexpression of mutated human genes that are known to be involved in the generation of amyloid-β in Alzheimer's... more
Biotransformation of flavonoid dimer FD18 resulted in an active metabolite FM04. It was more druggable because of its improved physicochemical properties. FM04 (EC50 = 83 nM) was 1.8-fold more potent than FD18 in reversing P-glycoprotein... more
This paper is dedicated to the memory of our friend and colleague Andreas Schmidlin P-glycoprotein (P-gp) is an ABC (ATP-binding cassette) transporter, which hydrolyses ATP and extrudes cytotoxic drugs from mammalian cells. P-gp consists... more
To achieve the objectives of the New Education Policy (NEP) 2020, a high-level committee comprising representatives from UGC, AICTE, NCVET, NIOS, CBSE, NCERT, Ministry of School Education and Learning, Ministry of Higher Education,... more
The family of ATP‐binding cassette (ABC) transporters is composed of several transmembrane proteins that are involved in the efflux of a large number of drugs including ivermectin, a macrocyclic lactone (ML) endectocide, widely used in... more
The rate, polarity, and symmetry of the flow of the plant hormone auxin are determined by the polar cellular localization of PIN-FORMED (PIN) auxin efflux carriers. Flavonoids, a class of secondary plant metabolites, have been suspected... more
Biotransformation of flavonoid dimer FD18 resulted in an active metabolite FM04. It was more druggable because of its improved physicochemical properties. FM04 (EC50 = 83 nM) was 1.8-fold more potent than FD18 in reversing P-glycoprotein... more
Gold nanoparticles have numerous applications, many of which are notable in industries. The biosynthesis of gold nanoparticles offers an easy, effective, green, and eco-friendly approach. In organisms capable of synthesizing... more
![Underlined sequences indicate the introduced restriction sites for NdeI in primers 1 and 5 and EcoRI in primers 2 and 6, respectively. TM: Melting temperature in degrees Celsius In this study, chromosomal DNA was isolated and trans- formed from T. scotoductus SA-01 (ATCC 700910) strain using an adapted SDS-proteinase procedure. E. coli TOP10 (Invitrogen) was used as a host for cloning of the protein while T. thermophilus HB27 (ATCC BAA-163) and E. coli BL21 (DE3) were used as hosts for the expression of the ABC transporter peptide-binding protein. T. scotoductus SA-01 (American Type Culture Collection) grown in glyc- erol stock (maintained at -80 °C), was cultured in Tryptone, Yeast Extract, Glucose (TYG) broth [18 g L bacteriologi- cal agar (Biolab), 5 g L~! tryptone (Biolab), 3 g L“! yeast extract (Biolab), and 1 g = glucose (Saarchem), pH 7.0], and incubated, under aerobic conditions for 24 h at 65 °C to reach the mid-exponential growth phase. E. coli TOP10 and E. coli BL21 (mesophilic host) cells were grown aerobically in Luria-Bertani (LB) medium with a final pH of 7.0 at 37 °C. The LB medium was supplemented with 100 ug mL™! ampicillin or 30 ug mL~! kanamycin as required (Sambrook et al. 1989). Thermus thermophilus HB27 (ATCC BAA-163), on the other hand, was used as a thermophilic host for the expression of the protein at 60-70 °C. The host, T: thermophi- lus was cultured from a 20% glycerol stock (maintained at -80 °C) as described (Erasmus et al. 2014). Thermus broth [8 g L7! tryptone (Biolab), 4 g L~! yeast extract (Biolab), 3 g L~! NaCl (Saarchem), and 2 M NaOH, pH 7.0] (Ramirez-Arcos et al. 1998), was used as the growth medium. The thermus broth medium was supplemented with 3 ug mL~! bleomycin or 30 ug mL“! kanamycin as required. Amplification of ABC transporter peptide-binding gene was carried out using the primers mentioned in Table 1. The PCR was performed in](https://figures.academia-assets.com/119793961/table_001.jpg)
This study was carried out to investigate the activity of ethylacetate extract of Zanthoxylum acanthopodium DC., fruit (EEZ) against doxorubicin-resistanced T47D cells.EEZ was prepared by maceration and T47D cells were treated by... more
Dasatinib (DAS) is a tyrosine kinase inhibitor (TKI) used in the treatment of chronic myeloid leukemia and in the management of ulcerative colitis (UC). Since some nutraceuticals (e.g. curcumin, olive oil, and cocoa extract) could alter... more
Chemoresistance is an important factor in the treatment failure of childhood acute lymphoblastic leukemia (ALL). One underlying mechanism of chemoresistance involves (over)expression of ATP-dependent drug efflux transporters such as... more
Bilirubin, the end-product of heme catabolism, circulates in non-pathological plasma mostly as a protein-bound species. When bilirubin concentration builds up, the free fraction of the molecule increases. Unbound bilirubin then diffuses... more
Secretion of cholesterol into bile is important for the elimination of cholesterol from the body. Thyroid hormone (TH) increases biliary cholesterol secretion and hepatic gene expression of adenosine triphosphate (ATP)-binding cassette,... more
We investigated the influence of nano-TiO 2 on the response to cadmium in the gills of the marine mussel Mytilus galloprovincialis in terms of accumulation and toxicity. Mussels were in vivo exposed to nano-TiO 2 , CdCl 2 , alone and in... more
Plant pathogens cause huge yield losses. Plant defense often depends on toxic secondary metabolites that inhibit pathogen growth. Because most secondary metabolites are also toxic to the plant, specific transporters are needed to deliver... more
(؊)Pantoprazole [(؊)PAN] accumulated in rat milk stereoselectively, and this accumulation was attributed to rat Abcg2 (rAbcg2). In contrast, flux experiments at 25 M showed that (؉)pantoprazole [(؉)PAN] was preferentially transported by... more
Pancreatic adenocarcinoma (PDAC) is one of the most deadly cancers in the Western world because of a lack of early diagnostic markers and efficient therapeutics. At the time of diagnosis, more than 80% of patients have metastasis or... more
The World Health Organisation recommends exclusive breastfeeding for the first six months of an infant's life and in combination with solid food thereafter. This recommendation was introduced based on research showing numerous health... more
Effect of CFTR correctors on the traffic and the function of intracellularly retained ABCB4 variants
Background & aimABCB4 is expressed at the canalicular membrane of hepatocytes. This ATP‐binding cassette (ABC) transporter is responsible for the secretion of phosphatidylcholine into bile canaliculi. Missense genetic variations of ABCB4... more
Lactobacillus plantarum is widely used in many fields, especially in medicine. In this study, besides the strain’s morphology (i.e, the colony and the cell under microscrope and scan electronic microscope), antibacterial activities... more
Conjugated linoleic acid (CLA) have been shown to exert numerous health benefits, including anti-carcinogenic, anti-atherogenic, anti-diabetic, antiobesity, cholesterol reducing, antioxidant, anti-microbial, immune system modulator and... more
X-linked Adrenoleukodystrophy (X-ALD) is caused by mutations in the ABCD1 gene resulting in the accumulation of very long chain fatty acids (VLCFA). X-ALD is the most common peroxisomal disorder with adult patients (male and female)... more
As the Transmission Line (TL) is an important part in the power system; this paper presents the design and practical implementation of overcurrent protection for threephase TL system using Programmable Logic Control (PLC). The PLC then... more
Trong nghiên cứu trước đây, chúng tôi nhận thấy vi khuẩn Vibrio vulnificus khá phổ biến trên cá nuôi bị xuất huyết ở tỉnh Thừa Thiên Huế. Vi khuẩn này là một mầm bệnh cơ hội trên người, có thể gây nhiễm trùng máu nguyên phát, nhiễm trùng... more
Sử dụng chế phẩm sinh học đối kháng tác nhân gây bệnh thay thế kháng sinh là định hướng nghiên cứu có tiềm năng cao trong ứng dụng kiểm soát vi khuẩn gây bệnh. Qua quá trình phân lập và sàng lọc ban đầu, chúng tôi thu được 17 chủng vi... more
Many non-lantibiotic bacteriocins of lactic acid bacteria are produced as precursors with N-terminal leader peptides different from those present in preproteins exported by the general sec-dependent (type II) secretion pathway. These... more
Many non-lantibiotic bacteriocins of lactic acid bacteria are produced as precursors with N-terminal leader peptides different from those present in preproteins exported by the general sec-dependent (type II) secretion pathway. These... more
Listeria monocytogenes (Lm) is a food-borne pathogen responsible for human listeriosis, an invasive infection with high mortality rates. Lm has developed efficient strategies for survival under stress conditions such as starvation and... more
The G subfamily of ABC (ATP-binding cassette) transporters of Leishmania include 6 genes (ABCG1-G6), some with relevant biological functions associated with drug resistance and phospholipid transport. Several studies have shown that... more
Aim/hypothesis. Type I diabetes is associated with altered hepatic bile formation and increased intestinal cholesterol absorption. The aim of this study was to evaluate whether altered expression of the ATP-Binding Cassette... more
Autoimmune hypothyroidism (AHT) is a widespread disease that disproportionately affects women over men. It is characterized by the presence of autoantibodies that lead to the dysfunction of the thyroid gland. The exact cause of this... more
The ability of sorbic acid and cycloheximide to inhibit yeast was investigated on nine yeast strains isolated from different sources. Sorbic acid was added to MRS agar at two concentrations 0.01 g/L (TCVN 5522:1991) and 1.4 g/L (TCVN... more
Tóm tắt: Vi khuẩn lactic (Lactic Acid Bacteria, LAB) đã được tìm thấy và sử dụng nhiều trong các sản phẩm lên men truyền thống, theo điều kiện tự nhiên. Nhiều nghiên cứu gần đây đã chứng minh vi khuẩn lactic có khả năng sinh các chất... more
Multi-drug resistance (MDR) leads to impaired treatment efficacy in all forms of malignancy. The main forms of MDR are thought to be mediated by the substrate transporting actions of certain adenosine triphosphate binding cassette (ABC)... more
Systematic unbiased high-throughput screening (HTS) of drug combinations (DCs) in well-characterized tumor cell lines is a data-driven strategy to identify novel DCs with potential to be developed into effective therapies. Four DCs from a... more
The immunophilin-like protein TWISTED DWARF1 (TWD1/FKBP42) has been shown to physically interact with the multidrug resistance/P-glycoprotein (PGP) ATP-binding cassette transporters PGP1 and PGP19 (MDR1). Overlapping phenotypes of... more
The ability of sorbic acid and cycloheximide to inhibit yeast was investigated on nine yeast strains isolated from different sources. Sorbic acid was added to MRS agar at two concentrations 0.01 g/L (TCVN 5522:1991) and 1.4 g/L (TCVN... more
TÓM TẮT Long An là một tỉnh có nền nông nghiệp chăn nuôi phát triển. Tuy nhiên, việc xử lí nước thải chăn nuôi không đúng cách đang làm ô nhiễm nguồn nước mặt ở khu vực này. Một trong những chất gây ô nhiễm đáng chú ý là tồn dư kháng sinh... more
Multidrug resistance (MDR) is the major complex mechanism that causes the failure of chemotherapy, especially with drugs of natural origin such as doxorubicin (DOX). Intracellular drug accumulation and detoxification are also involved in... more
α-Hexylcinnamaldehyde (HCA), a compound derived from cinnamaldehyde, was evaluated for its potential chemosensitizing properties. The cytotoxicity of HCA was tested against Caco-2, CCRF/CEM and CEM/ADR5000 human cancer cells. Furthermore,... more
The human ATP-binding cassette transporter ABCG2 is a key to anticancer resistance and physiological detoxification. However, the molecular mechanism of substrate transport remains enigmatic. A hydrophobic di-leucine motif in the ABCG2... more
SUMMARYMembers of the ATP-binding cassette (ABC) transporter family (P-glycoproteins, Half-transporters and Multidrug Resistant Proteins) potentially play a role in the development of anthelmintic resistance. The aim of this study was to... more
This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will... more