Vitrificación De Embriones Bovinos Producidos in Vitro

International Journal of Morphology, 2017

RESUMEN: El objetivo de este trabajo fue evaluar la fertilidad in vitro del semen bovino sexado (SX) vs. no sexado (NS) congelado-descongelado de dos toros Holstein, cada uno de la misma partida. Determinar el sexo de las crías significa un avance importante para la producción. El citómetro de flujo separa los espermatozoides X e Y por diferencia de ADN (4 % mayor en X), con 90 % de efectividad. Los complejos ovocito-cúmulus (COC) se obtuvieron de folículos de 2 a 8 mm de ovarios de frigorífico, se cultivaron para maduración 22 h en TCM-199 + 5 % de SFB + 10 % licor folicular bovino (LFB), en gotas de 100 µl, cubiertos con aceite mineral, en incubadora (38,5 ºC, 5 % CO 2 y 95 % de humedad). Posmaduración, se formaron al azar 4 grupos de COC los cuales fueron inseminados con NS y SX de los toros 1 y 2. Los COC se incluyeron en gotas de 100 µl a razón de 10 COC por gota de semen capacitado a una concentración de 2x10 6 espermatozoides/ml en todos los grupos, incubados durante 6 h. Posteriormente se cultivaron en CR1aa + 5 % SFB, en incubadora. A las 48 h se evaluó el clivaje y al día 7 el desarrollo embrionario. Los resultados fueron analizados con el Test de χ 2. Se encontró diferencias significativas en el toro 1 en el desarrollo embrionario a favor del NS (p<0,05). En el toro 2 no se encontró diferencias significativas en el clivaje ni en el desarrollo (p<0,05).

SPERMOVA, 2019

Vitrification is a new technique that has many potential advantages in mammalian oocytes and embryos. In the present work, the objective was to compare of post thawing survival rate by of blastocysts vitrified in vitro in two devices. Standardized procedure at the Laboratory of Reproductive Biotechnology, UNA La Molina was used in the experimental part, to produce in vitro bovine embryos from oocytes recovered from slaughterhouse ovaries. The viable oocytes were incubated at 38.5 ° C and 5% CO2 in 10 oocytes/group microdroplet (70 µl) covered with mineral oil, for 22 to 24 hours in maturation medium, 18 hours in fertilization medium and 7 days in culture medium. A total of 100 expanded blastocysts produced bovine day 7 of culture were used. Blastocysts were vitrified in Vitritip (n = 50) and Vitri-top (n= 50) devices, using standard media (Vitrogen®, Brazil) on vitrification, thawing and culture in vitro. Once thawed blastocysts from both groups were cultured in vitro for 3 hours at 38.5 ° C, 5% CO2 and 90% Hd. The comparison of both groups was performed using the statistical test Chi-square and using a significance level of 5%. No statistically significant differences (P>0.05) in the re-expansion rates in vitro post thawing between both devices during vitrification were observed post thawing survival rate, 52% (24/46) Vitri-tip and 48% (21/44) Vitri-top devices. Devices using vitrification closed in bovine embryos, can achieve low loss rate in low volume management, although the embryonic survival was not very impressive.

2015

The freezing of bovine embryos is a biotechnology applied to animal reproduction of this species, whose benefits is already a reality. The aim of this paper is to show aspects of great importance for freezing bovine embryos. Definition of freezing, freeze benefits of bovine embryos, freezing disadvantages of bovine embryos, variables to be taken into account for the success of the freezing of bovine embryos, cryoprotectants, a cryoprotectant functions: where the following described , conservation methods bovine embryos, conventional methods in the slow freezing of embryos, labeling straws in the freezing of bovine embryos, embryo thawing techniques, and crioinjuria One-step method.

2018

Revista de Investigación Agraria y Ambiental, 2013

Generalidades de la producción de embriones bovinos in vitro Overview of the production of bovine embryos in vitro Panorâmica da produção de embriões bovinos in vitro