The effects of zinc supplement on fertility in male mice

Pakistan Journal of Medical and Health Sciences, 2022

Aim: To determine the effect of zinc on spermatogenesis Methodology: the study was done at Shahida Islam Medical Complex, Lodhran. 60 albino rats were selected and divided into 4 groups each consisting of 15 albino rats each. The first control group-1 was feed with normal diet, while the group-2 and group-3 were treated with indomethacin and zinc respectively. Whereas the group-4 was treated with both zinc and indomethacin. This intervention was carried out for 12 weeks. Once the study was concluded, the rats were euthanized and histopathological analysis of the testes was carried out to determine the effect on spermatogenesis. Results: group 4 showed favorable results in which normal spermatogenesis could be observed, in group 2 however, focal loss of germ cells series was seen as well as focal basement membrane detachment. Conclusion: the study can safely say that zinc metal exhibits a protective role on the testes of albino rats Keywords: Spermatogenesis, Albino rats, Zinc, Indom...

Bangladesh Journal of Veterinary Medicine, 2020

Background: The effect of zinc supplementation on birth weight, litter size, total erythrocyte count (TEC), hemoglobin estimation, total leukocyte count (TLC), blood glucose level, abnormal sperm count and histopathology of testis in mice was evaluated. Methods: 45 albino mice were randomly divided into 3 equal groups viz. control group, A; 10 mg zinc/kg feed treated group, B; 20 mg zinc/kg feed treated group, C. Each group was comprised of 10 male mice and 5 female mice. Then they are allowed to breed for about 25 days. After breeding males were withdrawn from each group and treated with zinc for 30 days. Then male mice were transferred back to female cage and were allowed to breed for 25 days. Results: Mice of group B and C showed a significant increase (p<0.01) in total erythrocyte count (TEC), total leukocyte count (TLC), hemoglobin content, litter size and decreased abnormal sperm count. No significant increase in body weight, even a decrease in glucose content was recorded ...

Indian journal of urology : IJU : journal of the Urological Society of India

The objective of this study is to investigate the molecular mechanisms underlying the effects of zinc deficiency on spermatogenesis in the Sprague-Dawley (SD) rat. Three groups of eight adult male SD rats were maintained for 4 weeks on a normal diet as control, zinc deficient diet and zinc deficient diet with zinc supplementation of 28 mg zinc/kg body weight respectively. Using standard techniques, the following parameters were compared between the three groups of experimental animals at the end of 4 weeks: (a) Serum zinc, magnesium (Mg), copper (Cu), selenium (Se) and cadmium (Cd), (b) serum sex hormones, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPX), (c) interleukin-4 (IL-4), tumor necrosis factor-alpha (TNF-α), Bcl-2, Bax and caspase-3 expression in the testes, (d) assessment of apoptosis of testicular cells using electron microscopy and (e) testicular volume and histology using the orchidometer and Johnsen score, respectively. The zinc defici...

Innovative publication, 2016

Infertility is a worldwide problem with male factor contributing equally to infertility as females. Zinc is one of essential trace elements required for normal physiology of male reproduction and plays important role in spermatogenesis. The process of spermatogenesis is well studied and understood, but studies related to role of essential nutritional elements like zinc; necessary for normal spermatogenesis are not yet covered thoroughly in detail. The present review throws light on role of zinc in human spermatogenesis as well as its effect on male infertility. Method: The literature regarding male infertility and role of zinc in male reproduction was searched from various journals and published peer-reviewed articles on Pubmed, MEDLINE, Embase and Google Scholar till 2015.

Zinc is currently the trace minerals of greatest concern when considering the nutritional value of vegetarian. It plays several important roles in a biological system and considered as the closest thing to a nutritional aphrodisiac. In the present review various aspects related with zinc and male fertility were discussed. Zinc plays a key role in spermatogenesis from several perspectives and located primarily in the Lyding cells. It takes part in formation of sperm motility, influences directly on sperm morphology and plays an important part in capacitation. Various biological factors, heavy or regular use of alcohol, smoking and endocrine abnormalities are the major factors associated with de crea se level of seminal plas ma zinc leve l. Its bioavailability is highly influenced by phytate content of food material that makes non-vegetarian diet more preferable then vegetarian diet. Many medicinal plants as well as their products are now being use and marketed for correcting the Zinc deficiency. In present review it was realized that synergistic studies involving Zinc and phytate concentration and their impacts on sperm dynamics need to taken up.

2020

This study was carried out to assess the effect of dietary levels of zinc on semen indices, growth parameters and testicular sperm reserve of heterogeneous stock of male rabbits. Twenty male rabbits aged 6 mo old were randomly allotted to four groups of five males each and fed diets containing 0, 50, 100 and 150 mg of zinc gluconate per kg diet, respectively, for eight weeks. Semen was collected from the males weekly using an artificial vagina and semen volume (mL), spermatozoa motility (%), sperm concentration (×10 8 /mL) and live sperm cells (%) were assessed. Seminal plasma was separated from the semen by centrifugation, and its zinc concentration was determined using atomic absorption spectrometry. The weights of the males were taken weekly, and the daily feed intake was recorded. At the end of the feeding trial, the rabbits were stunned and slaughtered; the testes and epididymis were carefully removed and homogenised to determine sperm reserves using standard procedure. The res...

Journal of Obstetrics, Gynecology and Cancer Research, 2016

Background: Different studies found that zinc is necessary for sexual maturity, growth and fertility. But there are no distinct studies that clarify the role of zinc supplements on semen parameters. Objectives: The current study aimed to evaluate the zinc supplement therapeutic effects on semen samples of infertile males. Patients and Methods: The study comprised one-hundred-twenty sub fertile males. The study was a double-blinded placebocontrolled clinical trial. The subjects were randomly allocated to treatment with zinc supplement (n = 60) or placebo (n = 60) groups. Subjects in both groups were given 10 mL, three times daily. In order to determine the sperm concentration, Motility and morphology, standardized semen and blood samples were obtained before and after treatment, according to the World Health Organization (WHO) guidelines; semen morphology according to strict criteria, and blood and semen zinc concentration also were measured. Effects of the two interventions were evaluated in sub fertile males. Results: Sub fertile males demonstrated a significant increase (8.8 ± 7.4 × 10 6 cells/mL to 17.2 ± 13.5 × 10 6 cells/mL) in concentration and normal sperm in zinc group versus the placebo group. Blood serum zinc concentration increased in the interventional group significantly (P = 0.000), and also semen plasma zinc concentration increased significantly (P = 0.000). Conclusions: Normal sperm percentage and total sperm concentration increased after zinc sulfate treatment. The beneficial effect of zinc and all results of the current study opened new way to medical purposes and public health researches.

International Journal of Molecular Sciences

Several studies proposed the importance of zinc ion in male fertility. Here, we describe the properties, roles and cellular mechanisms of action of Zn2+ in spermatozoa, focusing on its involvement in sperm motility, capacitation and acrosomal exocytosis, three functions that are crucial for successful fertilization. The impact of zinc supplementation on assisted fertilization techniques is also described. The impact of zinc on sperm motility has been investigated in many vertebrate and invertebrate species. It has been reported that Zn2+ in human seminal plasma decreases sperm motility and that Zn2+ removal enhances motility. Reduction in the intracellular concentration of Zn2+ during epididymal transit allows the development of progressive motility and the subsequent hyper activated motility during sperm capacitation. Extracellular Zn2+ affects intracellular signaling pathways through its interaction with the Zn2+ sensing receptor (ZnR), also named GPR39. This receptor was found in...

Tikrit medical journal , 2011

Abstract The infertility rates vary between countries and from region to regions. It is documentedthat around 15% of married couples are infertile and that approximately 50% of infertility is due tomale factor. Systems that influenced by zinc include the reproductive, neurological, immune, skin,and gastro-intestinal systems. The study aimed to highlight the role of Zinc in management of menwith infertility.A longitudinal study in fifteen infertile men were treated with zinc sulphate capsule 200mg(equivalent to 50mg of zinc) once daily. History and clinical examination with semen analysis, aswell as serum and seminal plasma study for zinc, magnesium, Malondialdehyde (MAD), andglutathione. FSH, LH, and Testosterone also done. All these studies done both before and aftertreatment. Statistical analysis done.Serum zinc concentrations were not significantly increase of infertile men after treatmentzinc comparing with before treatment, while semen zinc and Mg, and semen Mg concentrationswere significantly increased after treatment. There is significant reduction in both serum andsemen MDA of infertile men after treatment. Significant increase in both serum and semenglutathione, and significant increase in the level of FSH, LH, and testosterone after treatment.These results supported by many study published that explained and conclude the effect of zinc onsemen quality.Zinc can be used as a single agent in treatment of male infertility

The Journal of Microbiology, Biotechnology and Food Sciences, 2018