PHYTOCHEMICAL ANALYSIS OF THE WHOLE PLANT OF Mimosa pudica (Linn.)
Prosanta Pal
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Abstract
Mimosa pudica (Linn.) is a creeping annual or perennial herb. It has been identified as lajjalu in Ayurveda and has been found to have antiasthmatic, aphrodisiac, analgesic, and antidepressant properties. M. pudica is known to possess sedative, emetic, and tonic properties, and has been used traditionally in the treatment of various ailments including alopecia, diarrhea, dysentery, insomnia, tumor, and various urogenital infections. As the whole plant being used to cure various diseases so we have extracted all the plant constituents from whole plant powder of Mimosa pudica (Linn.) using 50% ethanol following cold maceration technique. Preliminary phytochemical evaluation showed the presence ofcarbohydrates, alkaloids, proteins, amino acids, tannins, phenolics, flavonoids, steroids, fixed oil, mucilage and saponins. Among these compounds alkaloid was found to exhibit different pharmacological properties. So later we have separated and identified the alkaloids present in the 50% ethanolic whole plant extract and its fractions.
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IN VIVO PHARMACOLOGICAL INVESTIGATION OF MIMOSA PUDICA L.
Objective: To evaluate the anti-nociceptive, acute toxicity, gastro intestinal motility, anti-pyretic investigations of leaf extract of Mimosa pudica L. leaves in Swiss albino mice following oral administration. Methods: In-vivo anti-nociceptive activity test was evaluated by tail immersion test. In-vivo acute toxicity test was conducted using acute toxic class method. In-vivo gastrointestinal motility was determined by charcoal feces defecation time. In-vivo antipyretic activity test was evaluated by brewer’s yeast induced pyrexia. Results: In-vivo anti-nociceptive activity test shows that methanol & ethanol extracts (250 & 500 mg/kg b.w.) performed significant activity (p<0.05) in mice comparing to the standard drug diclofenac Na. In-vivo acute toxicity test was done on mice with methanol, ethanol and chloroform extracts (2000, 1000, 500 mg/kg b.w.) of Mimosa pudica leaf and no reaction or death occurred in mice during two weeks of observation. In-vivo gastrointestinal motility test indicates significant (p<0.01) increase in gastrointestinal motility by ethanol extracts of (250 & 500 mg/kg b.w.) comparing to the standard drug loperamide. In-vivo antipyretic activity test shows that methanol (250 & 500 mg/kg b.w.), ethanol (250 & 500 mg/kg b.w.) and chloroform (250mg/kg b.w.) extracts showed significant (p<0.05) reduction in temperature of mice comparing to the standard drug paracetamol. Conclusion: The result of the study indicates analgesic, antipyretic properties along with gastrointestinal motility stimulating effects. According to the acute toxicity study, the leaf extracts are safe up to 2000 mg/kg in-vivo concentration.
In-vivo Assessment of Neuropharmacological Activity of Methanol Bark Extract of Mimosa pudica in Mice
Advances in Pharmacology and Pharmacy, 2019
Background: Mimosa pudica, a common plant of Mimosoideae family has been used as Ayurvedic herbal medicine by Bangladeshi and Chinese to treat several diseases such as hemorrhoids, hair loss, arthritis, dysentery, leprosy, jaundice, leukoderma, asthma, uterine problems. The root and bark of the plant is said to display antimicrobial activity. Hence, gargling with a decoction of the root and bark of Mimosa pudica diluted in water may help to treat toothaches. Aim of the study: The present study was designed to evaluate the presence of phytochemical groups and to investigate the neuropharmacological activity of methanol extract of Mimosa pudica (MEMP). Methodology: The neuropharmacological activity was determined by hole cross and open field test using Swiss Albino mice as experimental animal. Results: Phytochemical analysis of Mimosa pudica extract indicated the presence of tannins, alkaloids, terpenoids and flavonoids. The sample showed dose-dependent sedative activity at the doses of 200 and 400 mg/kg body weight compared to standard drug Diazepam (1 mg/kg body weight). Conclusion: MEMP showed significant sedative activity demonstrating that the extract may be useful in the development of a new sedative and anti-anxiety drug.
Preliminary Pharmacognostic and Phytochemical Evaluation of Stems of Mimosa hamata (Willd)
International Journal of Scientific Research in Science and Technology, 2019
"Objective The aim of the present study is to investigate the pharmacognostic and phytochemical investigation of the Mimosa hamata (Willd.) is a flowering shrub of Mimosaceae family which is used in various traditional medicines to cure various diseases. Mimosa hamata (Willd.) and Mimosa pudica are also known as Touch-me-not plant. A wide range of chemical compounds including 4-ethyl-gallic acid; triterpinicsaponin A, B; ethylgallate; mimonoside A, B, C; etc have been isolated from this plant. Methods Morphological and microscopic characters, powder analysis, and extractive values of ethanolic extract of stem of Mimosa hamata and qualitative estimation of phytochemicals were determined. The pharmacognostical parameters such as total ash value, acid insoluble ash value and water soluble ash value, alcohol soluble extractive and water soluble extractive were also determined. Results The results of pharmacognostic analysis of stem of Mimosa hamata (Willd) have revealed the total ash 8.5 % , water soluble ash 0.5 %, water insoluble ash 1.5%, Moisture content 2.5 %, alcohol soluble extractive value 14.29 % and water soluble extractive value 9.75%. The preliminary phytochemical analysis of stem of showed the presence of flavonoids, carbohydrates, tannins etc. Conclusions It signifies that results revealed the presence of various bioactive constituents which could be exploited for their biopotential for medicinal purposes. "
Evaluation of antioxidant and cytotoxic activity of methanolic extract of Mimosa pudica leaves
The Pharma Innovation Journal, 2014
1. Department of Pharmacy, Primeasia University Dhaka, Bangladesh, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh. 2. Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh. 3. Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh. 4. Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh. 5. Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh.
IN VITRO ANTIOXIDANT ACTIVITIES AND IN VIVO ANTI-NOCICEPTIVE AND NEUROPHARMACOLOGICAL ACTIVITIES OF MIMOSA PUDICA
Mimosa pudica is the herb that shows sensation on touch. It has been identified as Lajjalu in Ayurveda and has been found to have anti-asthmatic, aphrodisiac, analgesic, anti-inflammatory and antidepressant activities. The aim of this study was to evaluate in-vitro antioxidant activity and in vivo biological activity considering anti-nociceptive assessment & neuropharmacological study include open field and swimming test. There is no scientific report on analgesic activity and neuropharmacological activity of Mimosa pudica, therefore the present study was undertaken to examine the possible in-vitro antioxidant activity of the extracts of Mimosa pudica was performed using DPPH free radical scavenging, cupric reducing antioxidant capacity, total antioxidant capacity, total phenol and total flavonoid content determination assays. The result of tests for in-vitro antioxidant activity indicates that petroleum ether extract shows maximum antioxidant activity. This plants leaf extracts also exhibit significant anti-nociceptive activity and neuropharmacological activity.
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